CN107548885A - Cordyceps militaris lighting culture - Google Patents
Cordyceps militaris lighting culture Download PDFInfo
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- CN107548885A CN107548885A CN201710764309.1A CN201710764309A CN107548885A CN 107548885 A CN107548885 A CN 107548885A CN 201710764309 A CN201710764309 A CN 201710764309A CN 107548885 A CN107548885 A CN 107548885A
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- fructification
- lampshade
- cordyceps militaris
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Abstract
The present invention relates to technical field of cultivation, discloses a kind of Cordyceps militaris lighting culture, comprises the following steps：Prepare culture medium：Cicada pupa powder, walnut shell powder, glucose, magnesium sulfate, vitamin B1 and water are stirred into pasty state together, the culture medium of 5cm × 5cm × 5cm brick-shaped is then made；Sterilization：Culture medium is sterilized；Seed covering：By in the pupal cell embedment culture medium after inoculation；Culture：Using cordyceps sinensis incubator culture 80 100 days, condition of culture is controlled by incubator, condition of culture is：Vegetative stage, moisture content in medium 60% -65% is made by filler pipe plus water, temperature is 18 DEG C -22 DEG C, and relative humidity is 60% -70%, closes LED；After fructification generative growth phase is reached, opening LED makes moisture content in medium 65% -70%, 20 DEG C -23 DEG C of temperature, relative air humidity 80% -90%；Harvest fructification：The fructification grown is produced with tweezers.The invention is intended to provide a kind of Cordyceps militaris lighting culture, to cultivate the Cordyceps militaris with linear pattern fructification.
Description
Technical field
The present invention relates to technical field of cultivation.
Background technology
Cordyceps militaris is Ascomycotina, ergot Zoopagales, Clavicipitaceae, the type sepecies of Cordyceps；Also known as northern Chinese caterpillar Fungus, north
Cordyceps militaris, cordyceps sinensis etc.；Cordyceps militaris is a kind of substitute of the cordyceps sinensis of artificial culture, and not only relative low price, contained
The cordycepin of being rich in nutrition value is also 16 times or so in wild cordyceps, has proven to have lymph cancer and part leukaemia
Positive therapeutic action, application prospect are very extensive.
The artificial pilot scale culture of Cordyceps militaris needs pupal cell kind by cordyceps are vaccinated with culture medium, cordyceps
Sprouted on pupal cell and form mycelium, mycelia again decomposes the various tissues and organ in pupal cell, is grown to serve as fructification.
Traditional Cordyceps militaris pilot scale culture device is in frame the vial or plastics box side for being inoculated with cordyceps
Cultivated on son, in Cordyceps militaris incubation, need to keep dark in the hyphostoma development stage, it is not necessary to illumination, and sub real
Body generative growth phase needs to keep certain illumination, ensures suitable indoor temperature and humidity.And Cordyceps militaris has phototaxis, lead
Fructification bending growth is caused, influences the harvesting, processing and appearance of fruiting bodies of cordyceps militaris.
The content of the invention
The invention is intended to provide a kind of Cordyceps militaris lighting culture, to cultivate the Cordyceps militaris with linear pattern fructification.
Cordyceps militaris lighting culture in this programme, comprises the following steps：
Step 1：Prepare culture medium：By weight by 50-60 part cicada pupas powder, 50-60 parts walnut shell powder, 5-10 parts glucose, 2-3
Part magnesium sulfate, 0.2-0.5 parts vitamin B1 and 15-30 part water stir into pasty state together, and 5cm × 5cm × 5cm brick is then made
Block culture medium；
Step 2：Sterilization：Room temperature is naturally cooled to after sterilizing more than 30min under the conditions of culture medium is put into 110 DEG C；
Step 3：Seed covering：By in the pupal cell embedment culture medium after inoculation；
Step 4：Culture：Using cordyceps sinensis incubator culture 80-100 days, cordyceps sinensis incubator include closing medium cassette and can be every
The lampshade of exhausted light, lampshade are fastened at the top of medium cassette, are provided with some through holes at the top of medium cassette, are connected with through hole
The culture tube of linear, it is LED that 100-240 is strangled that every culture tube is corresponded on lampshade inwall provided with one group of illuminance, culture
Base box is provided with filler pipe, filler pipe connection medium cassette, and filler pipe is extended to outside lampshade through lampshade；Culturing rack is provided with gas
Pipe, tracheae connect with the lampshade of each culture unit；Also include air wetting machine and air-conditioning, the air-out of air wetting machine and air-conditioning
Mouth is connected with tracheae；Condition of culture is：Vegetative stage, moisture content in medium 60% -65% is made by filler pipe plus water,
Temperature is 18 DEG C -22 DEG C, and relative humidity is 60% -70%, closes LED；After fructification generative growth phase is reached, with disappearing
Tweezers after poison fiddle with the sub- 1-3 entity of early growth period in every culture tube, open LED, pass through filler pipe plus water
Make moisture content in medium 65% -70%, 20 DEG C -23 DEG C of temperature, relative air humidity 80% -90%；
Step 5：Harvest fructification：The fructification grown is produced with tweezers, removes the culture medium of fructification connection.
The technical principle of this programme and have the beneficial effect that：Cicada pupa powder provides nitrogen as organic nitrogen source for the growth of Cordyceps militaris
Element.Containing abundant calcium, phosphorus, potassium element in walnut shell, be advantageous to gradually to be sustained out during Cordyceps militaris grows calcium,
Phosphorus, potassium element are beneficial to culture medium bricked is made for Cordyceps militaris absorption.Magnesium sulfate provides magnesium elements, vitamin B1 profit
In growing for mycelia.Inoculated after culture medium is sterilized, be advantageous to lift mycelial survival rate.
And culture tube is removed in cultivation stage, opening lampshade, after medium cassette and culture base tube sterilization, by culture medium
Box and pupal cell infected with cordyceps are put into medium cassette.The stage is sprouted in initial mycelium, keeps lighting source
In off-position, now keep dark in lampshade and medium cassette, by air wetting machine and air-conditioning adjusting air humidity and
Temperature, in vegetative stage temperature control at 18 DEG C -22 DEG C DEG C, relative humidity is maintained at 60% -70%, will by filler pipe
Moisture content in medium is controlled 60% -65%, in favor of mycelial growth.After the sporophore growth stage is reached, after sterilization
Tweezers the fructification of early growth period is fiddled with through hole, then every culture tube is arranged separately in each through hole again,
And allow in every culture tube and have 1-3 fructification, LED electrified light emitting is then given, and by temperature control at 20 DEG C -23 DEG C, it is empty
Gas relative humidity is maintained at 80% -90%, by moisture content in medium control 65% -70%, and appropriate Additional nutrient solution.Fructification
Under phototaxis and the guidance quality double action of culture tube, towards lighting source simple interest, finally grow into can harvest it is medicinal
Cordyceps militaris.
Further, the lampshade and the top surface of the medium cassette are in circular arc, and lampshade and the top surface of medium cassette
It is parallel.The medium cassette top surface of circular arc is bigger relative to the top surface surface area of planar shaped, more through holes can be set, beneficial to training
Support more fructifications；And the lampshade equal length for ensureing culture tube parallel with the top surface of medium cassette so that culture tube
Specification is identical, reduces production cost.
Further, the color temperature value of the LED is 5500K.Closer to the growth of natural light, more conducively fructification.
Further, the water filled in step 4 by filler pipe is distilled water.Avoid bringing other bacteriums into and influenceing fructification
Growth.
Further, the incubator in step 4 carries out cleaning and sterilizing before culture medium is put into the potassium permanganate liquid of 8% concentration.
Avoid the culture for having bacteria effect Cordyceps militaris in incubator.
Brief description of the drawings
Fig. 1 is the structural representation of cordyceps sinensis incubator.
Embodiment
Below by embodiment, the present invention is further detailed explanation：
Reference in Figure of description includes：Culturing rack 1, medium cassette 2, lampshade 3, tracheae 4, filler pipe 5, culture tube 6,
LED 7.
Embodiment one
Cordyceps militaris lighting culture, comprises the following steps：
Step 1：Prepare culture medium：By weight by 50 parts of cicada pupa powder, 50 parts of walnut shell powders, 5 parts of glucose, 2 parts of magnesium sulfate,
0.2 part of vitamin B1 and 15 parts of water stir into pasty state together, and the culture medium of 5cm × 5cm × 5cm brick-shaped is then made；
Step 2：Sterilization：Room temperature is naturally cooled to after sterilizing more than 30min under the conditions of culture medium is put into 110 DEG C, by incubator
Dried after carrying out cleaning and sterilizing with the potassium permanganate liquid of 8% concentration；
Step 3：Seed covering：By in the pupal cell embedment culture medium after inoculation；
Step 4：Culture：Using cordyceps sinensis incubator culture 80-100 days, as shown in Figure 1, it included closing to cordyceps sinensis incubator
Medium cassette 2 and the lampshade 3 that can completely cut off light, lampshade 3 are fastened on the top of medium cassette 2, and the top of medium cassette 2 is provided with some logical
Hole, is connected with the culture tube 6 of linear in through hole, and the top surface of lampshade 3 and medium cassette 2 is in circular arc, and lampshade 3 with
The top surface of medium cassette 2 is parallel, corresponds to that every culture tube 6 is 5500K provided with one group of color temperature value and illuminance is on the inwall of lampshade 3
The LED 7 of 100 Le, medium cassette 2 are provided with filler pipe 5, and filler pipe 5 connects medium cassette 2, and filler pipe 5 prolongs through lampshade 3
Extend outside lampshade 3；Culturing rack 1 is provided with tracheae 4, and tracheae 4 connects with the lampshade 3 of each culture unit；Also include air wetting
The air outlet of machine and air-conditioning, air wetting machine and air-conditioning is connected with tracheae 4；Condition of culture is：Vegetative stage, by adding
Water pipe 5 plus distilled water water make moisture content in medium 60%%, and temperature is 18 DEG C, relative humidity 70%, closes LED 7；Work as arrival
After fructification generative growth phase, 1 entity of son of early growth period is fiddled with every culture tube 6 with the tweezers after sterilization,
LED 7 is opened, moisture content in medium 65%, 20 DEG C of temperature, relative air humidity 80% are made by filler pipe 5 plus distilled water water；
Step 5：Harvest fructification：The fructification grown is produced with tweezers, removes the culture medium of fructification connection.
Embodiment two
With differing only in for embodiment one：
Step 1：Prepare culture medium：By weight by 60 parts of cicada pupa powder, 60 parts of walnut shell powders, 10 parts of glucose, 3 parts of magnesium sulfate,
0.5 part of vitamin B1 and 30 parts of water stir into pasty state together, and the culture medium of 5cm × 5cm × 5cm brick-shaped is then made；
Step 4：The illuminance of LED 7 is 240 Le, vegetative stage, contains culture medium by filler pipe 5 plus distilled water water
Water 65%, temperature are 22 DEG C, relative humidity 70%, close LED 7；After fructification generative growth phase is reached, with sterilization
Tweezers afterwards fiddle with 3 entities of son of early growth period in every culture tube 6, open LED 7, pass through filler pipe 5 plus distillation
Water water makes moisture content in medium 70%, 23 DEG C of temperature, relative air humidity 90%.
The fructification measurement curvature that Example one and embodiment two harvest, in below 0.05cm, have straight well
Linearly.
Claims (5)
1. Cordyceps militaris lighting culture, it is characterised in that：Comprise the following steps：
Step 1：Prepare culture medium：By weight by 50-60 part cicada pupas powder, 50-60 parts walnut shell powder, 5-10 parts glucose, 2-3
Part magnesium sulfate, 0.2-0.5 parts vitamin B1 and 15-30 part water stir into pasty state together, and 5cm × 5cm × 5cm brick is then made
Block culture medium；
Step 2：Sterilization：Room temperature is naturally cooled to after sterilizing more than 30min under the conditions of culture medium is put into 110 DEG C；
Step 3：Seed covering：By in the pupal cell embedment culture medium after inoculation；
Step 4：Culture：Using cordyceps sinensis incubator culture 80-100 days, cordyceps sinensis incubator include closing medium cassette and can be every
The lampshade of exhausted light, lampshade are fastened at the top of medium cassette, are provided with some through holes at the top of medium cassette, are connected with through hole
The culture tube of linear, it is LED that 100-240 is strangled that every culture tube is corresponded on lampshade inwall provided with one group of illuminance, culture
Base box is provided with filler pipe, filler pipe connection medium cassette, and filler pipe is extended to outside lampshade through lampshade；Culturing rack is provided with gas
Pipe, tracheae connect with the lampshade of each culture unit；Also include air wetting machine and air-conditioning, the air-out of air wetting machine and air-conditioning
Mouth is connected with tracheae；Condition of culture is：Vegetative stage, moisture content in medium 60% -65% is made by filler pipe plus water,
Temperature is 18 DEG C -22 DEG C, and relative humidity is 60% -70%, closes LED；After fructification generative growth phase is reached, with disappearing
Tweezers after poison fiddle with the sub- 1-3 entity of early growth period in every culture tube, open LED, pass through filler pipe plus water
Make moisture content in medium 65% -70%, 20 DEG C -23 DEG C of temperature, relative air humidity 80% -90%；
Step 5：Harvest fructification：The fructification grown is produced with tweezers, removes the culture medium of fructification connection.
2. Cordyceps militaris lighting culture according to claim 1, it is characterised in that：The lampshade and the medium cassette
Top surface is in circular arc, and lampshade is parallel with the top surface of medium cassette.
3. Cordyceps militaris lighting culture according to claim 1, it is characterised in that：The color temperature value of the LED is
5500K。
4. Cordyceps militaris lighting culture according to claim 1, it is characterised in that：Filled in step 4 by filler pipe
Water is distilled water.
5. Cordyceps militaris lighting culture according to claim 1, it is characterised in that：Incubator in step 4 is being put into training
Before foster base cleaning and sterilizing is carried out with the potassium permanganate liquid of 8% concentration.
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| CN201710764309.1A CN107548885A (en) | 2017-08-30 | 2017-08-30 | Cordyceps militaris lighting culture |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114600706A (en) * | 2022-03-14 | 2022-06-10 | 连云港市农业科学院 | Artificial culture method of cordyceps militaris |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010016736A (en) * | 1999-08-03 | 2001-03-05 | 정홍열 | Method for cultivating a paecilomyces japonica |
| CN102017865A (en) * | 2009-09-23 | 2011-04-20 | 北京农业生物技术研究中心 | Two-stage large-scale cordyceps militaris culture method |
| CN102301912A (en) * | 2011-07-01 | 2012-01-04 | 西藏月王生物技术有限公司 | Method for culturing Cordyceps militaris by using highland barley as main raw material |
| CN203735117U (en) * | 2014-01-10 | 2014-07-30 | 南京本派生物科技有限公司 | Improved cordyceps militaris culturing device |
| CN104160873A (en) * | 2014-08-06 | 2014-11-26 | 赵胜华 | Cultivation and management method for cordyceps militaris |
| CN204888087U (en) * | 2015-08-28 | 2015-12-23 | 延安大学 | Frame is cultivateed to chinese caterpillar fungus in north |
-
2017
- 2017-08-30 CN CN201710764309.1A patent/CN107548885A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010016736A (en) * | 1999-08-03 | 2001-03-05 | 정홍열 | Method for cultivating a paecilomyces japonica |
| CN102017865A (en) * | 2009-09-23 | 2011-04-20 | 北京农业生物技术研究中心 | Two-stage large-scale cordyceps militaris culture method |
| CN102301912A (en) * | 2011-07-01 | 2012-01-04 | 西藏月王生物技术有限公司 | Method for culturing Cordyceps militaris by using highland barley as main raw material |
| CN203735117U (en) * | 2014-01-10 | 2014-07-30 | 南京本派生物科技有限公司 | Improved cordyceps militaris culturing device |
| CN104160873A (en) * | 2014-08-06 | 2014-11-26 | 赵胜华 | Cultivation and management method for cordyceps militaris |
| CN204888087U (en) * | 2015-08-28 | 2015-12-23 | 延安大学 | Frame is cultivateed to chinese caterpillar fungus in north |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114600706A (en) * | 2022-03-14 | 2022-06-10 | 连云港市农业科学院 | Artificial culture method of cordyceps militaris |
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Application publication date: 20180109 |