CN106962025A - A kind of method that utilization LED lamplight cultivates Cordyceps militaris - Google Patents
A kind of method that utilization LED lamplight cultivates Cordyceps militaris Download PDFInfo
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- CN106962025A CN106962025A CN201710360092.8A CN201710360092A CN106962025A CN 106962025 A CN106962025 A CN 106962025A CN 201710360092 A CN201710360092 A CN 201710360092A CN 106962025 A CN106962025 A CN 106962025A
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- cordyceps militaris
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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- Biodiversity & Conservation Biology (AREA)
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- Forests & Forestry (AREA)
- Mushroom Cultivation (AREA)
Abstract
The present invention relates to a kind of method that utilization LED lamplight cultivates Cordyceps militaris.This method includes：The irradiation of 50 250 lux intensity is carried out with LED in Cordyceps militaris former base differential period, and/or the irradiation of 800 1200 lux intensity is carried out with LED in fruiting bodies of cordyceps militaris growth phase.LED lamplight of the present invention by certain strength and the selection on illumination opportunity, the complex optimum breeding method of Cordyceps militaris, make the increasing number of former base formation, with being tightly combined for culture medium, it is difficult to contaminate miscellaneous bacteria, sporophore growth is healthy and strong, yield is higher, bright-colored, there is the distinctive fragrance of stronger cordyceps sinensis.
Description
Technical field
The present invention relates to the technical field of Applied Biotechnology principle artificial culture fungi, and in particular to the training of Cordyceps militaris
Educate, relate more specifically to a kind of method that utilization LED lamplight cultivates Cordyceps militaris.
Background technology
Light can influence the growing of fungi, physiological period, metamorphosis and secondary as a kind of important envirment factor
The generation of metabolite.For Cordyceps militaris, illumination is the necessary condition of its sporophore growth development.At present, illumination for
The research of fruiting bodies of cordyceps militaris growth effect is continuing always.This research optimizes for the cultivation condition of fruiting bodies of cordyceps militaris, carries
High fruiting bodies of cordyceps militaris quality provides foundation.
Cordyceps militaris is a kind of rare medicinal fungi of China, has similar medicinal health care function to natural cordyceps.By
Increasingly exhausted in limited resource, natural prodcuts can not meet the market demand, and the fruiting bodies of cordyceps militaris manually cultivated is become as day
The best substitute of right cordyceps sinensis.Illumination plays an important role in Tissue Culture of Cordyceps militaris fructification, and light quality and light intensity are to pupa
Cordyceps militaris sporocarp formation and growth have important influence.
At present, LED is used for the example that Cordyceps militaris is cultivated, such as CN105532263A disclose a kind of LED it is blue,
The alternate LED of blue, white light lamp bead is pressed 1 meter of line-spacing, spacing 1 by the cultural method of the alternate illumination Cordyceps militaris of white light, this method
Between Chinese caterpillar fungus culture medium of the meter ampere in greenhouse, using as light source, beneficial to the growth of Cordyceps militaris；CN 205320652U are carried
Supply in a kind of LED indigo plants, the cultivating chamber of the alternate illumination Cordyceps militaris of white light, described cultivating chamber to be provided with Chinese caterpillar fungus culture medium, pupa worm
LED, its occupation mode and the same CN105532263A of effect are provided between careless culture medium.However, as previously described, although LED
Lamp has been used in the culture of Cordyceps militaris, but is used in the difference of Cordyceps militaris different growth phases, and LED light color is to pupa worm
Grass growth, particularly the role of growth of moment is not disclosed but, also not studied.
The content of the invention
In view of this, the present invention provides a kind of method that utilization LED lamplight cultivates Cordyceps militaris, including：In Cordyceps militaris former base
Differential period carries out the irradiation of 50-250 luxs intensity with LED, and/or in fruiting bodies of cordyceps militaris growth phase LED
Carry out the irradiation of 800-1200 luxs intensity.
LED lamplight of the present invention by certain strength and the selection on illumination opportunity, the complex optimum cultivation side of Cordyceps militaris
Method, makes the increasing number of former base formation, and being tightly combined for culture medium is difficult to contaminate miscellaneous bacteria, sporophore growth is healthy and strong, yield compared with
Height, it is bright-colored, there is the distinctive fragrance of stronger cordyceps sinensis.
Embodiment
The method that the present invention cultivates Cordyceps militaris using LED lamplight, including：Entered in Cordyceps militaris former base differential period with LED
The irradiation of row 50-250 luxs intensity, and/or with LED carry out 800-1200 luxs in fruiting bodies of cordyceps militaris growth phase
The irradiation of intensity.
In a preferred embodiment, the light that the LED is sent is feux rouges, gold-tinted or orange light.
In a preferred embodiment, in Cordyceps militaris former base differential period red or yellow LED lamp light irradiation,
The sporophore growth stage uses orange led lamp light irradiation.
In a preferred embodiment, red LED lamp light irradiation is used in Cordyceps militaris former base differential period, in fructification
Growth phase orange led lamp light irradiation.
In a preferred embodiment, daily light application time is 8-12 hours.
In a preferred embodiment, with LED to carry out 150-200 luxs in Cordyceps militaris former base differential period strong
The irradiation of degree.
In a preferred embodiment, in the cultivating process of Cordyceps militaris, nurturing an environment is closed, white light and day is excluded
The interference of light.
In a preferred embodiment, pure wheat is used for culture medium, and the pH value of culture medium is 5.5-7.2.
In a preferred embodiment, in Cordyceps militaris former base differential period, moisture content in medium is maintained at 60%-
65%, cultivation temperature is maintained at 16-25 DEG C, and relative air humidity is maintained at 60%-70%.
In a preferred embodiment, in Cordyceps militaris former base differential period, day and night temperature stimulation is carried out, i.e., is not being taken off
In the range of exerate pupa cordyceps sinensis growth temperature, make the temperature on daytime higher than the temperature in evening 5-10 DEG C.
In a preferred embodiment, in fruiting bodies of cordyceps militaris growth phase, moisture content in medium is maintained at 65%-
70%, cultivation temperature is maintained at 20-23 DEG C, and relative air humidity is maintained at 80%-90%.
In a preferred embodiment, suitably to be divulged information in the fructification generation phase, daily ventilation 2 times, each 20-
30min。
In a specific embodiment, feux rouges, gold-tinted or the orange light of the LED are provided by LED.
The present invention is further detailed explanation by the following examples, but these embodiments are not limited in any form
The present invention.Wherein, used material is existing commercially available prod, and the LED used is existing commercially available prod, used
Test method is normal experiment method unless otherwise specified.
In the examples below, pure wheat is used for culture medium, and according to routine operation inoculation and bacterium germination.Bacterium germination terminates
Afterwards, with 20 bottles for one group, it is placed under LED lamplight feux rouges, gold-tinted, orange light and cultivates respectively.Get hold of the pH value of culture medium.Sternly
Lattice control fruiting bodies of cordyceps militaris in early growth period, veraison, the temperature of the middle and late growth stage, humidity, intensity of illumination, ventilation
Between.
Embodiment 1
Pure wheat is used for culture medium, water is added according to conventional ratio, regulation culture medium pH value is 6.5.Culture medium is put into 120
The sterilizing of DEG C sterilizing cabinet, is down to after room temperature and is inoculated with, culturing room's bacterium germination is moved into after sealing.After bacterium germination terminates, with 20 bottles for one group, by it
It is placed under feux rouges and cultivates.
In vegetative stage, moisture content in medium is maintained at 65% or so, cultivation temperature is maintained at 20 DEG C -23 DEG C, sky
Gas relative humidity is maintained at 65% or so.In the sporophore growth stage, moisture content in medium is maintained at 65% or so, cultivation temperature
20 DEG C -23 DEG C are maintained at, relative air humidity is maintained at 85% or so.
Bacteria does not need illumination initial stage, with the bright LED feux rouges light stimulations of 200 luxs during former base formation, normally
Go out grass.The LED red light irradiations of 1000 luxs of sporophore growth.
Daily light application time is 10 hours.
Ventilation 2 times, each 25min daily.
Cordyceps militaris growth cycle is 50 days or so.Harvested, dried after fruiting bodies of cordyceps militaris is ripe, lucifuge storage.
Embodiment 2
Pure wheat is used for culture medium, water is added according to conventional ratio, regulation culture medium pH value is 6.5.Culture medium is put into 120
The sterilizing of DEG C sterilizing cabinet, is down to after room temperature and is inoculated with, culturing room's bacterium germination is moved into after sealing.After bacterium germination terminates, with 20 bottles for one group, it is placed in
Cultivated under gold-tinted.
In vegetative stage, moisture content in medium is maintained at 65% or so, cultivation temperature is maintained at 20 DEG C -23 DEG C, sky
Gas relative humidity is maintained at 65% or so.In the sporophore growth stage, moisture content in medium is maintained at 65% or so, cultivation temperature
20 DEG C -23 DEG C are maintained at, relative air humidity is maintained at 85% or so.
Bacterium germination does not need illumination initial stage, and with the LED lamplight yellow light of 85 luxs when former base is broken up, sporophore growth is used
The LED lamplight yellow light of 1000 luxs.
Daily light application time is 10 hours.
Ventilation 2 times, each 25min daily.
Cordyceps militaris growth cycle is 50 days or so.Harvested, dried after fruiting bodies of cordyceps militaris is ripe, lucifuge storage.
Embodiment 3
Pure wheat is used for culture medium, water is added according to conventional ratio, regulation culture medium pH value is 6.5.Culture medium is put into 120
The sterilizing of DEG C sterilizing cabinet, is down to after room temperature and is inoculated with, culturing room's bacterium germination is moved into after sealing.After bacterium germination terminates, with 20 bottles for one group, it is placed in
Cultivated under orange light.
In vegetative stage, moisture content in medium is maintained at 65% or so, cultivation temperature is maintained at 20 DEG C -23 DEG C, sky
Gas relative humidity is maintained at 65% or so.In the sporophore growth stage, moisture content in medium is maintained at 65% or so, cultivation temperature
20 DEG C -23 DEG C are maintained at, relative air humidity is maintained at 85% or so.
Bacterium germination does not need illumination initial stage, is irradiated when former base is broken up with the LED lamplight orange light of 85 luxs, sporophore growth is used
The LED lamplight orange light irradiation of 1000 luxs.
Daily light application time is 10 hours.
Ventilation 2 times, each 25min daily.
Cordyceps militaris growth cycle is 50 days or so.Harvested, dried after fruiting bodies of cordyceps militaris is ripe, lucifuge storage.
3 embodiments show that LED feux rouges can promote former base to break up more than, the former base differentiation that LED gold-tinteds, orange light promote
Quantity is relatively fewer.
1）Former base under feux rouges is yellow-white, and the quantity of former base formation is more, but can form the former base quantity of fructification
It is less.Very strong phototropism is shown in cordyceps sinensis growth course, cordyceps sinensis is tenderer, and combined closely with culture medium, be difficult to contaminate miscellaneous bacteria.
2）Former base color under gold-tinted is shallower, and negligible amounts, is light golden rod yellow, and cordyceps sinensis growing way is uniform.
3）The fruit-body formation cultivated under the conditions of orange light is good, and robust growth, yield is higher, bright-colored, there is stronger
The distinctive fragrance of cordyceps sinensis.
Conclusion：During Tissue Culture of Cordyceps militaris, fruit-body formation and growth are affected by many factors.Wherein illumination is
One of important factor of influence.Embodiment result shows that dim light, particularly feux rouges, gold-tinted or orange light have stimulation to former base differentiation
Effect, during Tissue Culture of Cordyceps militaris, using the processing of 50-250 luxs, preferably 150-200 luxs after mycelia sees that light turns yellow,
Former base can be promoted to break up, daily lighting delay number 8-12 hours, preferably 10 hours.Fruiting bodies of cordyceps militaris has very strong phototaxis,
Sporophore growth is good under certain light intensity.This test result indicates that, light intensity should be controlled in 1000 Le during Tissue Culture of Cordyceps militaris
Gram this left and right, daily lighting delay number be 8-12 hours.
Claims (10)
1. a kind of method that utilization LED lamplight cultivates Cordyceps militaris, it is characterised in that including：Used in Cordyceps militaris former base differential period
LED carries out the irradiation of 50-250 luxs intensity, and/or with LED carries out 800- in fruiting bodies of cordyceps militaris growth phase
The irradiation of 1200 lux intensity.
2. the method that utilization LED lamplight according to claim 1 cultivates Cordyceps militaris, it is characterised in that the LED is sent
Light be feux rouges, gold-tinted or orange light.
3. the method that utilization LED lamplight according to claim 2 cultivates Cordyceps militaris, it is characterised in that in Cordyceps militaris former base
Differential period red or yellow LED lamp light irradiation, orange led lamp light irradiation is used in the sporophore growth stage.
4. the method that utilization LED lamplight according to claim 3 cultivates Cordyceps militaris, it is characterised in that in Cordyceps militaris former base
Differential period uses red LED lamp light irradiation, and orange led lamp light irradiation is used in the sporophore growth stage.
5. the method that utilization LED lamplight according to claim 1 cultivates Cordyceps militaris, it is characterised in that daily light application time
For 8-12 hours；The irradiation of 150-200 luxs intensity is carried out with LED in Cordyceps militaris former base differential period.
6. the method that the utilization LED lamplight according to claim any one of 1-5 cultivates Cordyceps militaris, it is characterised in that in pupa
In the cultivating process of cordyceps sinensis, nurturing an environment is closed, the interference of white light and daylight is excluded.
7. the method that the utilization LED lamplight according to claim any one of 1-5 cultivates Cordyceps militaris, it is characterised in that use
Pure wheat is culture medium, and the pH value of culture medium is 5.5-7.2.
8. the method that the utilization LED lamplight according to claim any one of 1-5 cultivates Cordyceps militaris, it is characterised in that in pupa
Cordyceps sinensis former base differential period, moisture content in medium is maintained at 60%-65%, and cultivation temperature is maintained at 16-25 DEG C, relative air humidity
It is maintained at 60%-70%；In fruiting bodies of cordyceps militaris growth phase, moisture content in medium is maintained at 65%-70%, and cultivation temperature is maintained at
20-23 DEG C, relative air humidity is maintained at 80%-90%.
9. the method that the utilization LED lamplight according to claim any one of 1-5 cultivates Cordyceps militaris, it is characterised in that in pupa
Cordyceps sinensis former base differential period, day and night temperature stimulation is carried out, i.e., in the range of Cordyceps militaris growth temperature is not departed from, makes the temperature on daytime
Degree is higher than the temperature in evening 5-10 DEG C.
10. the method that the utilization LED lamplight according to claim any one of 1-5 cultivates Cordyceps militaris, it is characterised in that in pupa
Cordyceps sinensis former base is broken up and the sporophore growth stage, daily ventilation 2 times, each 20-30min.
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| CN201710360092.8A CN106962025A (en) | 2017-05-20 | 2017-05-20 | A kind of method that utilization LED lamplight cultivates Cordyceps militaris |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109983984A (en) * | 2018-01-03 | 2019-07-09 | 博尚生技实业（湛江）有限公司 | Far infrared Antrodia camphorata breeding apparatus and method |
| CN113303165A (en) * | 2021-04-15 | 2021-08-27 | 四川菌绿生态农业科技有限公司 | Seafood mushroom planting method |
| CN114600706A (en) * | 2022-03-14 | 2022-06-10 | 连云港市农业科学院 | Artificial culture method of cordyceps militaris |
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| CN101707966A (en) * | 2009-04-14 | 2010-05-19 | 徐正华 | Living Chinese caterpillar fungi artificially cultivated by using larvae as hosts and method for cultivating same |
| CN102433259A (en) * | 2011-12-16 | 2012-05-02 | 湖北民族学院 | Full-wavelength light source controllable biological culture device |
| CN103843583A (en) * | 2014-03-06 | 2014-06-11 | 刘彦君 | Industrialized production method of green cordyceps militaris |
| CN105493887A (en) * | 2015-12-05 | 2016-04-20 | 正源堂（天津）生物科技有限公司 | Rejuvenation culture method of cordyceps militaris |
| CN105532263A (en) * | 2015-12-23 | 2016-05-04 | 遵义鸿霖生物技术有限公司 | Cultivation method for Cordyceps militaris through alternate illumination with blue and white lights of LED |
| CN205320652U (en) * | 2015-12-23 | 2016-06-22 | 遵义鸿霖生物技术有限公司 | LED is blue, cultivation room of alternate illumination pupa chinese caterpillar fungus of white light |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101707966A (en) * | 2009-04-14 | 2010-05-19 | 徐正华 | Living Chinese caterpillar fungi artificially cultivated by using larvae as hosts and method for cultivating same |
| CN102433259A (en) * | 2011-12-16 | 2012-05-02 | 湖北民族学院 | Full-wavelength light source controllable biological culture device |
| CN103843583A (en) * | 2014-03-06 | 2014-06-11 | 刘彦君 | Industrialized production method of green cordyceps militaris |
| CN105493887A (en) * | 2015-12-05 | 2016-04-20 | 正源堂（天津）生物科技有限公司 | Rejuvenation culture method of cordyceps militaris |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109983984A (en) * | 2018-01-03 | 2019-07-09 | 博尚生技实业（湛江）有限公司 | Far infrared Antrodia camphorata breeding apparatus and method |
| CN113303165A (en) * | 2021-04-15 | 2021-08-27 | 四川菌绿生态农业科技有限公司 | Seafood mushroom planting method |
| CN114600706A (en) * | 2022-03-14 | 2022-06-10 | 连云港市农业科学院 | Artificial culture method of cordyceps militaris |
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Application publication date: 20170721 |