KR0179455B1 - The method for cultivating mushroom - Google Patents

The method for cultivating mushroom Download PDF

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KR0179455B1
KR0179455B1 KR1019960032339A KR19960032339A KR0179455B1 KR 0179455 B1 KR0179455 B1 KR 0179455B1 KR 1019960032339 A KR1019960032339 A KR 1019960032339A KR 19960032339 A KR19960032339 A KR 19960032339A KR 0179455 B1 KR0179455 B1 KR 0179455B1
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cordyceps
insects
spawn
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fungus
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이재일
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D417/00Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/02Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/12Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/395Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/41Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
    • A61K31/425Thiazoles
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/395Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44Non condensed pyridines; Hydrogenated derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D277/00Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/60Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings condensed with carbocyclic rings or ring systems
    • C07D277/62Benzothiazoles
    • C07D277/68Benzothiazoles with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached in position 2
    • C07D277/82Nitrogen atoms

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  • Medicinal Chemistry (AREA)
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Abstract

본 발명은 건조된 다양한 곤충을 물에 넣고 끓인 다음 수분함량을 60-65%가 되도록 조절한 뒤 종균병에 2/3 정도 넣고 고압살균하여 냉각시킨 후 무균실에서 중간 배양된 동충하초버섯 종균을 투입하여 곤충과 동충하초버섯 종균이 잘 혼합되도록 교반하여 배양하는 것을 특징으로 하는 곤충만을 이용한 동충하초버섯의 대량 재배방법에 관한 것으로, 본 발명에 의하면 손쉽게 구할 수 있는 곤충을 이용하여 약효가 탈월한 어떠한 동충하초버섯 종균이라도 인공적으로 손쉽게 대량 재배할 수 있는 뛰어난 효과가 있다.In the present invention, various dried insects are put in water, boiled, and then adjusted to a water content of 60-65%, and then placed in a spawn bottle about 2/3 of the fungus, and then cooled by autoclaving, and then inoculated with a fungus incubated medium in a sterile chamber. The present invention relates to a method for cultivating cordyceps mushrooms using only insects, characterized by culturing by stirring and mixing the insects and cordyceps fungi spawn well, and according to the present invention, any insecticidal fungus that departs from the medicinal effect using easily available insects Even artificially has an excellent effect that can be easily grown in large quantities.

Description

곤충만을 이용한 동충하초버섯의 대량 재배방법Mass cultivation method of cordyceps mushroom using only insects

본 발명은 곤충만을 이용한 동충하초버섯의 대량재배방법에 관한 것으로, 더욱 상세하게는 번데기, 누에, 지네, 벌, 매미, 메뚜기 등의 각종 곤충만을 배지로 하여 동충하초버섯을 대량재배하는 방법에 관한 것이다.The present invention relates to a method for cultivating cordyceps mushrooms using only insects, and more particularly, to a method for mass-cultivating cordyceps mushrooms using only various insects such as pupa, silkworm, centipede, bee, cicada and grasshopper as a medium.

동충하초(Cordyceps militaris)는 곤충에 기생하는 버섯의 일종으로, 동충하초란 말은 겨울에는 곤충의 몸에 살면서 양분을 흡수하여 곤충을 죽게한 후, 여름이 되면 죽은 곤충의 몸에서 버섯을 만든다는 뜻에서 고대 중국에서 유례된 것이다. 이러한 동충하초는 거의 모든 종류의 곤청에서 볼 수 있고, 유충, 번데기 등 모든 성장단계에 걸쳐 곤충의 몸 속에 침입하며, 일단 곤충의 체내로 침입하면, 곤충의 양분을 흡수하여 균사가 만들어지고, 결국 곤충의 몸 전체가 전체가 백색의 균사로 가득 메워진다. 이때 곤충의 몸은 전혀 부패하지 않고, 동충하초 또한 원형 그대로 보존된다.Cordyceps militaris is a kind of mushrooms parasitic to insects. In the winter, cordyceps militaris live in insects, absorbing nutrients to kill insects, and in summer they make mushrooms from dead insects. It is analogous to China. These cordyceps can be found in almost all kinds of stalks, invade the insect body through all stages of growth such as larva and pupa, and once invaded by insects, they absorb the nutrients of insects and create mycelia, and eventually insects The whole body is filled with white mycelium. At this time, the body of the insect does not rot at all, Cordyceps sinensis is also preserved intact.

동충하초의 종류로서 대표적인 것은 번데기 동충하초(Cordyceps militaris(Vuill_ Fr.) 풍뎅이 동충하초(C. scarabaceicola Y. Kobayasi), 노린재 동충하초(C. nutans Pat.), 벌 동충하초(C. sphecocephala(KI.) Sacc.), 거품벌레 동충하초(C. tricentri Yasuda), 눈꽃 동충하초(Paecilomyces fabrinosus) 등을 포함한다.Cordyceps sinensis (Cordyceps militaris (Vuill_ Fr.) beetle cordyceps (C. scarabaceicola Y. Kobayasi), C. nutans Pat., Bee cordyceps (C. sphecocephala (KI.) Sacc.) , C. tricentri Yasuda, Paecilomyces fabrinosus, and the like.

동충하초는 수분 10.84%, 지방 8.4%, 조단백 25.32%, 탄수화물 28.90%, 화분 4.1%로 구성되어 있으며, 지방 성분으로는 포화지방산 13%, 불포화지방산 82.2%가 함유되어 있고 비타민 B2도 100g 당 0.29mg 함유되어 있다(성재모, 버섯건강요법, 가림출판사, P178-192).Cordyceps sinensis is composed of 10.84% moisture, 8.4% fat, 25.32% crude protein, 28.90% carbohydrate, and 4.1% pollen.The fat component contains 13% saturated fatty acids and 82.2% unsaturated fatty acids, and 0.29mg of vitamin B2. (Sung Jae Mo, Mushroom Health Therapy, Garim Publisher, P178-192).

동충하초는 중국에서 예로부터 황제의 불로장수 비약으로 전승되어 오던 것으로, 체력을 증강시킴으로써 감기, 폐결핵, 만성기침, 천식, 발작, 빈혈, 허약, 만성의 성기능 장애, 고혈압에 탁월한 치료효과를 나타내며 피로회복에도 효과가 우수하다.Cordyceps sinensis has been handed down from ancient times as the Emperor's longevity elixir in China, and by improving stamina, it has excellent therapeutic effects on colds, pulmonary tuberculosis, chronic cough, asthma, seizures, anemia, weakness, chronic sexual dysfunction and high blood pressure. The effect is excellent.

또한 동충하초에는 매우 강력한 면역기능 증강작용이 있다는 사실이 최근에 증명되었는데, 동충하초에서 추출한 영약액은 유기체의 면역기능을 현저하게 강화시키고 체액면역과 세포면역에 대해서도 촉진효과가 있어, 종양과 바이러스 가염에 대한 유기체의 저항력을 높인다. 특히, 최근의 연구에 의하면 동충하초에 종양억제율 83%의 대단히 높은 항암성분이 있음이 발견되었다. 이와 관련하여 일본 도호쿠 대학 약학부 곤도 교수는 동충하초중에 에르고스테롤퍼옥사이드라는 암을 강하게 공격하는 약리성분이 함유되어 있다는 것을 규명했다.In addition, it has recently been proved that Cordyceps sinensis has a very powerful immune function. The medicinal solution extracted from Cordyceps sinensis significantly enhances the immune function of the organism and promotes body fluid immunity and cellular immunity. Increase the organism's resistance to In particular, a recent study found that Cordyceps sinensis has a very high anticancer component with a tumor suppression rate of 83%. In this regard, Professor Kondo of the Faculty of Pharmacy, Tohoku University, Japan, discovered that ergosterol peroxide contains a pharmacological ingredient that strongly attacks cancer during cordyceps.

이러한 성분은 부작용이 전혀 없고 저항력증강, 세균 또는 바이러스 감염에 대해 뛰어난 작용을 나타낸다.These ingredients have no side effects and show excellent action against resistance, bacterial or viral infections.

동충하초는 상술한 효능 외에도 마약중동의 해독제로서의 효과가 우수하고, 그 밖에도 곤충에 기생하는 속성 때문에 효과적인 해충방제를 제공하면서도 환경오염을 예방할 수 있는 생물농약으로서의 효용도 크다.Cordyceps sinensis is excellent as an antidote for drug-middle east in addition to the above-mentioned efficacy, and besides, it is also useful as a biopesticide that can prevent environmental pollution while providing an effective pest control agent due to its parasitic properties on insects.

동충하초는 5월에서 8월 사이에 주로 채집되며, 채취한 동충하초는 건조하여 삶아서 달여 마시거나, 기타의 방법으로 복용할 수 있다.Cordyceps sinensis is collected mainly between May and August, and the harvested cordyceps can be dried, boiled, or consumed by other means.

그러나, 이러한 경이적인 효능을 갖는 동충하초는 발견이 쉽지 않은 게 흠인데, 온도변화에 민감한 반응을 보이기 때문에 채집이 매우 까다롭고, 더욱이 성장 시기별로 침입하는 균도 달라 어려운 점이 많다. 따라서, 그 가격이 매우 높아 일반인이 이용하는데 많은 어려움이 있어 이러한 동충하초를 양산할 수 있는 방법이 요청되어 왔다.However, Cordyceps sinensis with such phenomenal efficacy is not easy to find, because it is sensitive to temperature changes, it is very difficult to collect, and even more difficult to invade by the growth period. Therefore, since the price is very high, there are many difficulties in using by the general public, and a method for mass production of Cordyceps sinensis has been requested.

종래, 동충하초버섯을 제외한 일반적인 버섯의 인공 배양방법으로는 톱밥을 배지로 사용하는 것이 공지되어 있는데, 이와 같이 톱밥을 이용하여 동충하초버섯 종균을 배양하는 경우에는 균사체까지는 형성되나 자실체는 결코 형성되지 않기 때문에 그 동안 동충하초버섯 종균의 인공배양은 불가능한 것으로 간주되어 왔다.Conventionally, it is known to use sawdust as a medium for artificial culture of mushrooms other than Cordyceps mushrooms. In this way, when cultivating Cordyceps fungi spawn using sawdust, even mycelia are formed, but fruiting bodies are never formed. In the meantime, artificial culture of Cordyceps mushroom spawn has been considered impossible.

또한, 인위적으로 자실체를 형성하기 위하여 감자한천배지와 누에번데기를 이용하거나 또는 쌀과 누에번데기를 이용하여 이를 살균한 다음 삼각플라스크나 플라스틱통에 충진한 후 종균을 접종하여 20-21℃로 배양하는 방법이 공지(성재모, 한국의 동충하초 PP228∼229, 교학사, 1996)된 바 있으나, 이는 곤충만을 배지로 사용하지 않고 탄수화물과 혼합된 혼합배지를 사용하므로서 진정한 약효를 가지지 못하는 동충하초 유사품에 불과하고 또 대량재배에 한계가 있었다.In addition, sterilize it by using potato agar medium and silkworm chrysalis or by using rice and silkworm chrysalis to artificially form fruiting bodies, and then inoculate spawn seedlings and incubate at 20-21 ℃. Although the method has been known (Seong Jae-mo, Cordyceps sinensis PP228-229, Kyohaksa, 1996), it is only similar to Cordyceps sinensis which does not have true medicinal effects by using a mixed medium mixed with carbohydrates instead of using only insects as a medium. There was a limit to cultivation.

본 발명의 목적은 상기와 같은 문제점을 감안하여 다년간 연구 끝에 안출한 것으로 각종 곤충만을 순수배지로 이용하여 신비의 약효를 갖는 동충하초버섯의 자실체를 인공적으로 대량으로 배양할 수 있는 동충하초버섯 자실체의 대량배양방법을 제공함에 있다.The object of the present invention has been devised after many years of research in view of the above problems, the mass culture of Cordyceps sinensis fruiting bodies capable of artificially cultivating the fruiting bodies of Cordyceps sinensis mushrooms having a mysterious effect using only various insects as pure medium. In providing a method.

상기 본 발명의 목적은 건조된 다양한 곤충을 물을 넣고 끓인 다음 수분함량을 60∼65%가 되도록 조절한 뒤 종균병에 넣고 고압살균하여 냉각시킨 후 무균실에서 중간 배양된 동충하초버섯 종균을 투입하여 곤충과 동충하초버섯 종균이 잘 혼합되도록 교반하여 배양하고 이와 비교하기 위하여 톱밥배지를 사용하거나 종균병에 투입량을 변경하여 실시하고 이를 평가함으로써 달성하였다.The object of the present invention is to put a variety of dried insects to boil water and then adjust the water content to 60 ~ 65% and then put in a spawn bottle and cooled by autoclaving to put the fungus incubated medium in Cordyceps fungi medium in a clean room It was achieved by stirring and incubating so as to mix well with Cordyceps fungi spawn and using a sawdust medium or changing the input amount in the spawn bottle to compare with this.

이하에서 본 발명의 구체적인 구성 및 작용을 더욱 상세히 설명한다.Hereinafter will be described in more detail the specific configuration and operation of the present invention.

본 발명에 의해 동충하초버섯 종균을 배양하는 경우에는 먼저 건조된 곤충을 물을 넣고 끓인 다음 수분함량을 60∼65%가 되도록 조절하여야 한다. 이때 건조된 곤충은 반드시 끓여 주어야만 수분함량을 상기 범위내로 조절할 수 있으며, 수분함량이 상기 범위를 벗어나면 균사가 약해지고 배양되는 버섯균의 품질이 저하되므로 상기 범위내로 조정하는 것이 중요하다.In the case of cultivating the Cordyceps mushroom spawn according to the present invention, the dried insects are first boiled with water and then adjusted to have a water content of 60 to 65%. At this time, the dried insects must be boiled to adjust the water content within the above range, and if the water content is out of the above range, it is important to adjust within the above range because the mycelia become weak and the quality of the cultured mushrooms is degraded.

이어서, 수분함량이 조절된 곤충을 종균병에 넣고 120℃ 이상의 온도로 고압 살균하여 냉각시킨 후 무균실에서 중간 배양된 동충하초버섯 종균을 투입하여 곤충과 동충하초버섯 종균이 잘 혼합되도록 교반하여 배양한다. 이때 곤충과 동충하초버섯 종균이 잘 혼합되도록 흔들어 주지 않으면 수분이 고르게 공급되지 않아 수분이 부족한 부분에서는 양질의 동충하초버섯을 수득할 수 없다. 따라서, 본 발명에서는 곤충배지에 동충하초버섯 종균을 접종한 후 흔들어주는 것이 필수적이며 이를 위해 수분함량이 조절된 곤충을 종균병에 가득 채우지 않고 2/3정도 충진하는 것이 바람직하다.Subsequently, the insects whose moisture content is controlled are placed in a spawn bottle, and sterilized by autoclaving at a temperature of 120 ° C. or higher, and then, the fungus is incubated in a sterile chamber, and then cultured by stirring so that the insect and the fungus mushroom spawn are well mixed. At this time, the insects and the Cordyceps mushroom spawn are not shaken well so that the moisture is not supplied evenly, so that the quality of the Cordyceps fungi can not be obtained in the part where the moisture is insufficient. Therefore, in the present invention, it is essential to shake after inoculating the insecticidal mushroom spawn seedling on the insect medium, and for this purpose, it is preferable to fill the insecticide with a water content of about 2/3 without filling the spawn disease.

곤충배지상에서 동충하초버섯 종균을 배양할 때의 온도는 23∼25℃가 바람직하며, 배양온도가 23℃ 미만이면 배양속도가 느려 비경제적이며, 이와 반대로 배양온도가 25℃를 초과하는 경우에는 배양속도는 빨라지나 최종적으로 수득되는 버섯균 활성이 약해지는 문제점이 발생한다. 따라서 본 발명에서 동충하초버섯 종균의 배양온도는 상기 범위내인 것이 필요하다.The temperature of cultivating Cordyceps fungi spawn on insect medium is preferably 23-25 ° C. If the culture temperature is less than 23 ° C, the culture rate is uneconomical. On the contrary, if the culture temperature exceeds 25 ° C, the culture rate The problem arises that the faster the fungus activity is finally obtained. Therefore, in the present invention, the culture temperature of Cordyceps mushroom seedlings needs to be within the above range.

본 발명에서 배지로 사용하는 곤충은 특별히 제한되지 않고 번데기, 노린재, 벌, 매미, 잠자리 등 어느 것이나 사용할 수 있으나, 가격 및 효과의 견지에서 번데기가 가장 바람직하다.Insects used as a medium in the present invention is not particularly limited and may be any one of pupa, stink bug, bee, cicada, dragonfly, etc., but pupa is most preferred in terms of price and effect.

본 발명의 작용은 정확하게 밝혀지지는 않았지만 동충하초버섯은 곤충에 기생하는 버섯의 일종이므로, 본 발명에서와 같이 곤충을 배지로 사용하면 곤충 체내에 포함되어 있는 활성대사 물질이 동충하초버섯의 생장을 돕는 것으로 추측된다.Although the action of the present invention has not been precisely identified, since the fungus is a parasitic mushroom parasitic insects, when the insect is used as a medium as in the present invention, the active metabolic material contained in the insect body to help the growth of cordyceps fungi I guess.

본 발명 방법에 의해 배양되는 동충하초버섯은 영양강장제, 항암제, 마약중독 해독제 등의 각종 의약품 원료와 해충을 퇴치하기 위한 생물농약으로 이용될 수 있다.Cordyceps mushrooms cultivated by the method of the present invention can be used as a raw material for various pharmaceuticals such as nutrient tonics, anticancer agents, drug addiction detoxification agents and biological pesticides to combat pests.

본 발명 방법에 의하면 벌, 매미, 잠자리 등의 곤충에 어떠한 동충하초 버섯균을 투입하여도 동충하초버섯을 손쉽게 배양해 낼 수 있다. 따라서, 손쉽게 구할 수 있는 곤충을 이용하면 약효가 탁월한 어떠한 동충하초버섯균이라도 인공적으로 쉽게 배양할 수 있다.According to the method of the present invention, it is possible to easily cultivate cordyceps mushrooms even if any cordyceps fungi are added to insects such as bees, cicadas, dragonflies and the like. Therefore, by using easily available insects can be easily cultured artificially any Cordyceps mushroom fungi excellent in efficacy.

또한, 본 발명 방법에 의하면 그 동안 인공적으로 배양하기 곤란한 것으로 간주되어 왔던, 탁월한 약효를 갖는 동충하초버섯을 양산할 수 있어, 일반인들도 쉽게 이용할 수 있게 되는 이점을 수득할 수 있다.In addition, according to the method of the present invention, it is possible to mass-produce cordyceps mushrooms having excellent medicinal effects, which have been considered difficult to artificially cultivate in the past, thereby obtaining an advantage of being readily available to the general public.

이하, 실시예를 들어 본 발명을 더욱 상세히 설명하나 본 발명이 하기 실시예로 제한되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited to the following Examples.

[실시예 1]Example 1

건조된 번데기 150g을 물 1,500ml에 넣고 60분간 끓인 다음 탈수기를 이용해서 수분 함량이 62%가 되도록 탈수하여 850cc 종균병에 2/3 정도 넣고 121℃로 80분간 고압살균해서 20℃까지 냉각시킨 후 무균실에서 중간 배양된 번데기 동충하초버섯(Cordyceps militaris(Vuill) Fr.) 종균을 투입하고 번데기와 투입된 번데기 동충하초버섯 종균이 혼합되도록 흔들어준 뒤 24℃에서 배양하였다. 그 결과 20일만에 번데기 동충하초버섯 자실체를 수득하였다.150 g of dried pupa in 1,500 ml of water, boiled for 60 minutes, dehydrated to 62% of water using a dehydrator, put about 2/3 in 850cc spawn bottle, and autoclaved at 121 ℃ for 80 minutes and cooled to 20 ℃. Inoculate the pupal cordyceps fungi (Cordyceps militaris (Vuill) Fr.) spawn incubated in a clean room and incubated at 24 ° C after shaking to make the pupa and the fungus cordyceps fungi added. As a result, pupa cordyceps fungi fruiting body was obtained in 20 days.

[실시예 2]Example 2

건조된 누에 150g을 물 1,500ml에 넣고 60분간 끓인 다음 탈수기를 이용해서 수분 함량이 62%가 되도록 탈수하여 850cc 종균병에 2/3 정도 넣고 121℃로 80분간 고압살균해서 20℃까지 냉각시킨 후 무균실에서 중간 배양된 노린재 동충하초버섯(C. nutans Pat.) 종균을 투입하고 누에와 투입된 노린재 동충하초버섯 종균이 혼합되도록 흔들어준 뒤 24℃에서 배양하였다. 그 결과 20일만에 노린재 동충하초버섯 자실체를 수득하였다.150 g of dried silkworms were put in 1,500 ml of water, boiled for 60 minutes, dehydrated using a dehydrator to reach a water content of 62%, placed in a 850cc spawn bottle for about 2/3, and autoclaved at 121 ℃ for 80 minutes and cooled to 20 ℃. Inoculated C. nutans Pat. Mushrooms cultured in a sterile chamber and incubated at 24 ° C. after shaking the silkworms and the introduced C. nutans fungus mushroom spawn. As a result, the stink bug Cordyceps sinensis fruiting body was obtained in 20 days.

[실시예 3]Example 3

건조된 지네 300g을 물 3,000ml에 넣고 60분간 끓인 다음 탈수기를 이용해서 수분 함량이 62%가 되도록 탈수하여 850cc 종균병에 각각 2/3 정도씩 넣고 121℃로 80분간 고압살균해서 20℃까지 냉각시킨 후 무균실에서 한 개의 종균병에는 중간 배양된 번데기 동충하초버섯(Cordyceps militaris(Vuill) Fr.) 종균을 투입하고, 다른 종균병에는 중간 배양된 노린재 동충하초버섯(C. nutans Pat.) 종균을 투입하여 잘 흔들어준 뒤 24℃에서 배양하였다. 그 결과 20일만에 두 개의 종균병 모두에서 각각의 동충하초버섯 자실체를 수득하였다.Put 300 g of dried centipede in 3,000 ml of water, boil for 60 minutes, dehydrate to reach 62% water content using a dehydrator, add 2/3 of each to a 850cc spawn bottle, and autoclave at 121 ℃ for 80 minutes and cool to 20 ℃. In the spawning room, one spawn bottle is fed with a medium cultured pupa, Cordyceps militaris (Vuill) Fr., and another spawn bottle with a medium cultured stink bug Cordyceps fungus (C. nutans Pat.) Shake well and incubated at 24 ℃. As a result, each of the fungus fruiting bodies of the Cordyceps sinensis was obtained in both seedling disease in 20 days.

[비교예 1]Comparative Example 1

종래의 방법대로 톱밥 150g에 물을 분무하여 수분함량이 62%가 되도록 조정하여 850cc 종균병에 채워 넣고 121℃로 80분간 고압살균해서 20℃까지 냉각시킨 후 무균실에서 중간 배양된 번데기 동충하초버섯(Cordyceps militaris(Vuill) Fr.) 종균을 투입하고 24℃에서 배양하였다. 그 결과 동충하초버섯 종균의 균사체는 형성되었으나 자실체는 형성되지 않았다.After spraying water to 150g of sawdust according to the conventional method, the water content is adjusted to 62%, and filled into 850cc spawn bottle, sterilized by autoclaving at 121 ℃ for 80 minutes, cooled to 20 ℃, and then cultured pupa cordyceps (Cordyceps) militaris (Vuill) Fr.) spawn was added and incubated at 24 ° C. As a result, the mycelia of the Cordyceps mushroom spawn were formed, but the fruiting bodies were not formed.

[비교예 2]Comparative Example 2

건조된 번데기 150g을 물 1,500ml에 넣고 60분간 끓인 다음 탈수기를 이용해서 수분 함량이 62%가 되도록 탈수하여 850cc 종균병에 가득 넣고 121℃로 80분간 고압살균해서 20℃까지 냉각시킨 후 무균실에서 중간 배양된 번데기 동충하초버섯(Cordyceps militaris(Vuill) Fr.) 종균을 투입하고 흔들어주지 않고 그대로 유지하여 24℃에서 배양하였다. 그 결과 동충하초버섯 종균이 전체적으로 형성되지 않고 일부분에서는 수분부족으로 자실체가 형성되지 않았다.150g dried chrysalis was put in 1,500ml of water and boiled for 60 minutes, then dehydrated to 62% water content using a dehydrator, filled in 850cc spawn bottle, autoclaved at 121 ℃ for 80 minutes, cooled to 20 ℃ and then medium in a clean room. Cultured pupa cordyceps fungi (Cordyceps militaris (Vuill) Fr.) spawn was added and maintained without shaking and incubated at 24 ℃. As a result, Cordyceps spp. Did not form as a whole, and in some parts, fruiting bodies were not formed due to lack of moisture.

이상에서 설명한 바와 같이 본 발명은 공충만을 효과적으로 이용하여 동충하초버섯을 재배하는 방법을 제공하는 효과가 있다. 또한 본 발명은 곤충만을 이용하므로 진정한 의미의 약효가 있는 동충하초버섯을 제공하는 효과가 있으므로 약용버섯 재배산업상 매우 유용한 발명인 것이다.As described above, the present invention has an effect of providing a method for cultivating Cordyceps sinensis mushrooms using only coworms effectively. In addition, the present invention is very useful invention in the medicinal mushroom cultivation industry because it has the effect of providing the Cordyceps sinensis mushroom with the true meaning of using only insects.

Claims (1)

건조된 곤충을 물에 넣고 끓인 다음 수분함량을 60∼65%가 되도록 조절한 뒤 종균병에 2/3 정도 넣고 고압살균하여 냉각시킨 후 무균실에서 중간 배양된 동충하초버섯 종균을 투입하여 곤충과 동충하초버섯 종균이 잘 혼합되도록 교반한 다음 23∼25℃에서 배양하는 것을 특징으로 하는 곤충만을 이용한 동충하초버섯의 대량 재배방법.Put dried insects in water and boil, adjust the water content to 60 ~ 65%, put it in spawn bottle about 2/3, cool it by autoclaving, and put the cauliflower fungus medium incubated in a clean room. A mass cultivation method of Cordyceps sinensis mushroom using only insects, characterized in that the mixture is stirred so that the seed is well mixed.
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Cited By (7)

* Cited by examiner, † Cited by third party
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KR19990078866A (en) * 1999-08-14 1999-11-05 김종수 Artificial method of production for paecilomyces japonica by using the dried silkworm
KR20010069295A (en) * 2001-03-02 2001-07-25 이재일 A method of cultivation Cordyceps militaris using a silkworm cocoon for culture medium
KR100349579B1 (en) * 1999-08-03 2002-08-22 정홍열 Method for cultivating a paecilomyces japonica
KR100361165B1 (en) * 1999-07-03 2002-11-22 까치마을영농조합법인 New Cordyceps sp. using Xylotrupes dichotomus larva as host and production thereof
KR100404333B1 (en) * 2001-07-25 2003-11-03 에이치엔엠바이오(주) A method of cultivating cordyceps based upon a snail or a mud snail
KR100453840B1 (en) * 2002-10-10 2004-10-20 김승보 Production method of Cordyceps scarabaeicola Kobayasi using Allomyrina dichotoma
KR20200077650A (en) * 2018-12-20 2020-07-01 씨엔지유기농 영농조합법인 Spore suspension comprising insect extract and preparation method of allomyrina dichotoma Cordyceps with increased cordycepin content using this

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KR100762819B1 (en) * 2006-06-15 2007-10-04 주식회사 그린 아그로텍 A method for effective fruiting body production of paecilomyces japonica using bombus terrestris and preparation of healthy food comprising the same

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100361165B1 (en) * 1999-07-03 2002-11-22 까치마을영농조합법인 New Cordyceps sp. using Xylotrupes dichotomus larva as host and production thereof
KR100349579B1 (en) * 1999-08-03 2002-08-22 정홍열 Method for cultivating a paecilomyces japonica
KR19990078866A (en) * 1999-08-14 1999-11-05 김종수 Artificial method of production for paecilomyces japonica by using the dried silkworm
KR20010069295A (en) * 2001-03-02 2001-07-25 이재일 A method of cultivation Cordyceps militaris using a silkworm cocoon for culture medium
KR100404333B1 (en) * 2001-07-25 2003-11-03 에이치엔엠바이오(주) A method of cultivating cordyceps based upon a snail or a mud snail
KR100453840B1 (en) * 2002-10-10 2004-10-20 김승보 Production method of Cordyceps scarabaeicola Kobayasi using Allomyrina dichotoma
KR20200077650A (en) * 2018-12-20 2020-07-01 씨엔지유기농 영농조합법인 Spore suspension comprising insect extract and preparation method of allomyrina dichotoma Cordyceps with increased cordycepin content using this

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