KR20200077650A - Spore suspension comprising insect extract and preparation method of allomyrina dichotoma Cordyceps with increased cordycepin content using this - Google Patents

Abstract

The present invention relates to a spore suspension comprising an insect extract as an effective ingredient and a method for preparing Allomyrina dichotoma cordyceps with an enhanced large amount of cordycepin by using the same. According to the present invention, the spore suspension may contain the insect extract as an effective ingredient so as to increase the content of cordycepin of Allomyrina dichotoma cordyceps. In addition, according to the present invention, a method for preparing Allomyrina dichotoma cordyceps may use Allomyrina dichotoma so that fungi grow similar to a natural state to kill insects and generate cordyceps, and also may use the spore suspension to increase useful ingredients of cordyceps and establish optimal culture conditions, thereby preparing Allomyrina dichotoma cordyceps containing a high content of cordycepin.

Description

Spore suspension comprising insect extract and preparation method of allomyrina dichotoma Cordyceps with increased cordycepin content using this}

The present invention relates to a spore suspension containing an insect extract as an active ingredient and a method for preparing longevity scarab cordyceps in which codycepin content is enhanced in a large amount using the same.

Longevity scarab ( Allomyrina dichotoma ) belongs to the insect family (Insecta) Coleoptera (Dynastidae) by taxonomy, and is a one-flowered insect that completes its life cycle once a year. Fable. After the imago, they mate and spawn from the end of August to September, and hatch into a 1-star larva after about 2 weeks. It is the largest and well-known insect among scarab species in Korea. The male's head has long horns, and the back of the chest has horns, which is the biggest characteristic of the appearance of the beetle. It is mainly found in oak trees in the forest and sucks gin flowing from old trees. The shape of the whole body is slightly elongated oval, and the color is black or brown and glossy. Only males have horns, females do not have horns, and they are smaller in size than males and have hairs throughout the back. They have sharp claws on their feet, so they climb trees well. Antennae short and blunt. Jangsu beetle having these characteristics is known to be effective in liver diseases such as cirrhosis and liver cancer, and is known to be effective in curing a weak body and curing various adult diseases.

On the other hand, Cordyceps sinensis is a medicinal mushroom, winter insects, insect insects, summer insects, grass grasses, and in winter, lurks in the insect's body to absorb nutrients and kill insects, When the environment is right in the summer, it is called Dongchunghacho because it makes mushrooms like grass. About 750 species of Cordyceps sinensis have been published in the academic world and are known to have been used since 3,000 years ago. It has been said that it has been said to have been used as a potion for the longevity, and has been taken by Qin Shi Huang, Poppy and Deng Xiaoping for longevity. The representative efficacy of Cordyceps sinensis has been revealed through research papers with many effects such as anti-cancer action, immune enhancement, liver function improvement, hypertension, anti-aging, diabetes improvement, heart disease, and nutritious tonic. Is becoming. Recently, as consumption of healthy foods has rapidly increased, interest in Cordyceps has been reexamined, and health supplements using Cordyceps have been developed.

However, since the number of cordyceps collected in the natural state is limited and the number is reduced due to environmental pollution and climate change, we are having a lot of difficulties in developing products using cordyceps and analyzing useful pharmacological components and developing them as medicinal materials. . In this situation, the collection of Cordyceps sinensis resources, development of collected strains and superior strains, and the development of new species of Cordyceps sinensis using them and the development of stable mass artificial production technology are in desperate need.

Accordingly, the present inventors have researched to manufacture cordyceps sinensis with enhanced content of codysepin as a useful ingredient, and when cultivated by injecting a spore suspension containing a specific substance into a live longevity scarab pupa, the codysepin content is significantly improved. Also, the present invention was completed by establishing optimal culture conditions for the production of such longevity beetles.

Korean Registered Patent No. 10-1835383 Korean Registered Patent No. 10-1810854

Accordingly, an object of the present invention is to provide a spore suspension capable of enhancing useful components of Cordyceps sinensis by containing a specific substance.

Another object of the present invention is to provide a method for preparing cordyceps sinensis beetle with enhanced codysepin content using the spore suspension.

Another object of the present invention is to provide a cordyceps sinensis beetle enhanced codycepin content produced by the above method.

In order to achieve the object of the present invention as described above,

The present invention provides a spore suspension containing the spore and insect extract of Cordyceps sinensis as an active ingredient.

In one embodiment of the present invention, the cordyceps strain may be Cordyceps militaris .

In one embodiment of the present invention, the insect extract may be a hot water extract of a white-spotted flower larvae or a hot water extract of rice locusts.

Of the present invention In one embodiment, the spores of the Cordyceps sinensis can be included in the spore suspension at 10 ⁶ to 10 ⁷ CFU/mL.

In addition, the present invention comprises the steps of (a) injecting and inoculating the spore suspension into a live longevity scarab; And (b) incubating the injection-inoculated longevity scarab for 25 to 45 days under conditions of 60 to 70% humidity and 26 to 30°C, providing a method for preparing cordyceps sinensis with enhanced codysepin content. do.

In one embodiment of the present invention, after the step (b) (c) further comprising the step of culturing mycelia for 15 to 45 days at 85 ~ 95% humidity and 20 ℃ ~ 30 ℃ temperature mycelium cultured longevity beetle can do.

In addition, the present invention provides a longevity scarab cordyceps with enhanced codycepin content prepared by a production method comprising the steps (a) to (c).

In one embodiment of the present invention, the longevity beetle Cordyceps may contain codicepin in an amount of 10 to 30 mg/g.

The spore suspension according to the present invention can increase the codycepin content of longevity scarab beetles by including the insect extract as an active ingredient. In addition, the method of manufacturing longevity scarab cordyceps according to the present invention can induce that fungi grow and kill insects and generate cordyceps similar to the natural state by using live longevity scarab, and useful components of cordyceps by using the spore suspension. In addition to being able to increase, by establishing the optimum culture conditions can be prepared longevity scarab cordyceps containing a high content of codysepin.

Figure 1a shows the results of inducing fruiting bodies 20 days after inoculation of spore suspension into live longevity beetle larvae.
Figure 1b shows the results of inducing fruiting bodies 20 days after inoculation with a spore suspension in a live brown larva.
Figure 1c shows the results of inducing fruiting bodies 20 days after inoculation with a spore suspension in a live white-spotted flower larvae.
Figure 1d shows the results of inducing fruiting bodies 50 days after inoculation of spore suspension into live paired crickets.
Figure 2a is a photograph showing the process of inoculating spore suspension in a live longevity scarab pupa.
Figure 2b is a photograph showing the 5th day after the spore suspension injection inoculation on the live longevity scarab pupa.
Figure 2c is a photograph showing the 20th day after the spore suspension injection inoculation on a live longevity scarab pupa.
Figure 2d is a photograph showing the 40 days after the spore suspension injection inoculation on the live longevity scarab pupa.
Figure 2e is a photograph showing the 45th day after inoculation of the spore suspension injection into the live longevity scarab pupa.
Figure 2f is a photograph showing the 55th day after inoculation of spore suspension injection into a live beetle pupae.
Figure 2g is a photograph showing the 60 days after inoculation of spore suspension injection into a live beetle pupae.
Figure 2h is a photograph showing the 80th day after the spore suspension injection inoculation on the live longevity scarab pupa.
Figure 2i is a photograph showing the 90th day after inoculation of the spore suspension injection into a live beetle pupae.
Figure 3 is a result of measuring the codycepin content by culture day by temperature conditions in the mycelial culture stage of the longevity scarab beetle (2a: 20 ℃, 2b: 25 ℃, 2c: 30 ℃).
Figure 4 is a result of measuring the codycepin content by the number of culture days by temperature conditions at the stage of longevity beetle cordyceps fruiting stage (3a: 20 ℃, 3b: 25 ℃, 3c: 30 ℃).
Figure 5 is a result of measuring the codycepin content of the longevity scarab cordyceps grown by type of spore suspension inoculated.

The present invention is characterized by providing a spore suspension containing the spore and insect extract of Cordyceps sinensis as an active ingredient.

'Dongchunghacho' referred to in this specification is a medicinal mushroom, winter dong (벌레), insect worm (蟲), summer summer (Summer), grass grass (草), in the insect body in winter, absorbs nutrients and absorbs insects It is called Dongchunghacho because it makes mushrooms like grass when the environment is right in the summer, and it has long been known as a great medicine with ginseng and antler.

Cordyceps is known to contribute to the physiological activity of cordyceps because it contains various low molecular substances such as polysaccharides, cordycepin, adenosine, lovastatin, ergosterol, and various unsaturated fatty acids. Among them, nucleic acid derivatives such as codycepin and adenosine are contained in high concentrations, and they are also used as main indicators of Cordyceps sinensis. In particular, codycepin is known as an active ingredient exhibiting physiological activities such as anti-cancer, antioxidant and anti-inflammatory, and has been reported as a major pharmacological component of Cordyceps sinensis in Korea.

The fungus forming cordyceps has host specificity depending on its type. For example, Cordyceps cicadae hosts cicada and Ophiocordyceps sinensis or Paecilomyces hepialid ), Lecanicillium , etc., host bat moth larvae. On the other hand, Cordyceps militaris ) has a wide range of host specificities and can host various insects.

The term “insect extract” referred to in the present specification may be an extract of an insect such as a white spotted flower larvae or a rice locust, but the type of insect is not limited thereto.

In one embodiment of the present invention, the insect extract is added to 1 g of distilled water to 100 g of white spotted flower larvae or rice locusts, extracted with hot water at a temperature of 100 to 120° C. for 6 to 24 hours, filtered to remove impurities, and Insect extracts can be prepared by centrifugation at a rate to recover the supernatant.

In the present invention, in the technique of artificially cultivating Cordyceps sinensis by directly inoculating spore suspension into Cordyceps sinensis based on living insects, when the insect extract as described above is included as a component of Spore suspension, the codysepin content of Cordyceps remarkably It was confirmed that it can be increased.

Accordingly, the present invention provides a spore suspension containing the spore and insect extract of Cordyceps sinensis as an active ingredient.

In one embodiment of the present invention, the Cordyceps sinensis may be Cordyceps militaris , and spores may be included in the spore suspension at 10 ⁶ to 10 ⁷ CFU/mL.

In addition, the present invention provides a method of manufacturing cordyceps sinensis beetle enhanced codysepin content.

Longevity scarab ( Allomyrina dichotoma ) belongs to the insect family (Insecta) Coleoptera (Dynastidae) in taxonomy and is a one-pest insect that completes its life cycle once a year.

In the present invention, the longevity scarab beetle can be prepared by inoculating and incubating the spore suspension of the present invention mentioned above with the live longevity scarab pupa as the host.

The method of manufacturing the longevity scarab cordyceps of the present invention comprises: (a) inoculating a live longevity scarab with a spore suspension; And (b) incubating the inoculated longevity scarab for 25 to 45 days under conditions of 60 to 70% humidity and 26 to 30°C.

The step (a) of the present invention is a step of injecting and inoculating a spore suspension into live longevity scarab, and in detail, directly inoculating a spore suspension containing spore and insect extract of Cordyceps sinensis as an active ingredient into live longevity scarab. to be.

The step (a) of the present invention is first, it is different from the prior art in that it uses a spore suspension containing spores and insect extracts of Cordyceps sinensis as an active ingredient, and secondly, Cordyceps sinensis is used for live insects (longevity scarab). By inoculating and cultivating, it is not only different from the prior art in that it is manufactured in the same way as the natural cordyceps, but third, it is different from the prior art in that it uses pupae, not larvae of the beetle, as the host.

For reference, when the spore suspension is injected with the longevity scarab larva as the host, it contains a lot of internal moisture, and thus, it was not suitable for the growth of Cordyceps sinensis inside the larva after inoculation. In addition, it was confirmed that the seed larvae are large in size, but have very high activity and immunity, so that the inoculum was not re-exposed or inoculated even after inoculation. Thus, the present inventors tried to inoculate longevity scarab larvae, but the mycelium was not properly cultured, and the contamination rate was very high, so it was unsuccessful. On the other hand, the longevity scarab was suitable for growing Cordyceps mycelium because it was the time when the inside solidified.

In addition, the present inventors experimented with long-lived scarab larvae, white spotted flower larvae, brown larva larvae, paired crickets, and silkworm pupae as living host insects that inject spore suspensions, and as a result, fruit body shedding was observed in all specimens except silkworm pupae. It was confirmed that it did not occur (see FIGS. 1A to 1D ).

Therefore, it was confirmed that longevity scarab larvae, white spotted flower larvae larvae, brown larvae larvae, and paired crickets are undesirable in inoculating and cultivating Cordyceps sinensis by injecting spore suspension into living insects, and that longevity scarab pupae and silkworm pupae are possible. However, since the silkworm pupae is inherently small in size, the fruiting body is small compared to the longevity scarab pupae and has a small codysepine content (in the case of the longevity pupae, the codysepine content of about 3 times higher than that of the silkworm pupae is shown), the present invention Essence used longevity scarab pupa as a live host insect suitable for the culture of Cordyceps sinensis.

The step (b) of the present invention is a step of mycelial cultivation of the longevity scarab inoculated with Cordyceps sinensis, and in detail, the inoculated longevity scarab obtained through the step (a) is 60-70% humidity and 26-30 It is a step of incubating mycelium for 25 to 45 days at a temperature condition of ℃.

The method for preparing longevity scarab beetle of the present invention further comprises (c) incubating mycelial cultured longevity scarab for 15 to 45 days under conditions of 85 to 95% humidity and 20 to 30° C. It can contain. Step (c) of the present invention is a step of culturing the mycelium cultured longevity beetle fruiting body.

In the present invention, the best culture conditions for strain culture and optimum culture conditions for fruiting the fruiting body to produce longevity scarab beetle containing a high content of codysepin by directly inoculating the Cordyceps sinensis with live longevity scarab pupae as the host Was established, and these culture conditions were first identified in the present invention.

Therefore, the present invention is differentiated in that the optimal conditions for each of the strains and fruiting bodies have been identified for the production of longevity scarab beetle, which has a higher codycepin content than the prior art.

In addition, the present invention provides a cordyceps longevity beetle enhanced codycepin content produced by the above method.

Longevity scarab cordyceps prepared by the above method of the present invention may include codicepin in an amount of 10 to 30 mg/g.

Hereinafter, the present invention will be described in more detail through examples. These examples are intended to illustrate the present invention more specifically, but the scope of the present invention is not limited to these examples.

< Example 1>

<1-1> Host Preparations

In the present invention, live longevity scarab was used as a host.

In detail, longevity scarab pupae (without skins such as wings, before the fable begins) were used as the host. Longevity scarab larvae and pupae were purchased from “Osangkin Sector Co., Ltd.” and “Dongui Bio Co., Ltd.”.

<1-2> Preparation of Cordyceps sinensis

In the present invention, Cordyceps was collected from nature and separated, and Cordyceps was obtained through morphological and molecular biological methods. militaris ). Molecular biological identification was commissioned by Macrogen Co., Ltd. and sequence analysis of the 18S rRNA region was performed, and the isolates identified as “Sejong 006” were assigned their own strain numbers to conduct experiments.

< Example 2>

Preparation of spore suspension

<2-1> Sterilized water Preparation of used spore suspension

Pupa Cordyceps militaris ) strain was passaged on a PDA (Potato Dextrose Agar, Difco) solid medium and cultured for 21 days in a 25°C incubator. 3 ml of sterile water was dispensed into the cultured plate and scraped using a sterilized spreader. The scraped spore solution was filtered using a 10 μm-sized filter to filter out the mycelium and harvest only the spores. The harvested spores were counted using a hemocytometer, and the spore suspension used for inoculation was used by diluting sterile water to a concentration of 10 ⁷ CFU/mL.

<2-2> Mineral water Preparation of used spore suspension

Pupa Cordyceps militaris ) strain was passaged on a PDA (Potato Dextrose Agar, Difco) solid medium and cultured for 21 days in a 25°C incubator. 3 ml of sterilized mineral water was dispensed into the cultured plate and scraped using a sterilized spreader. The scraped spore solution was filtered using a 10 μm-sized filter to filter out the mycelium and harvest only the spores. The harvested spores were counted using a hemocytometer, and the spore suspension used for inoculation was used by diluting sterilized mineral water to a concentration of 10 ⁷ CFU/mL.

<2-3> White spotted Flower larva Hot water extract Preparation of used spore suspension

First, a white spotted flower larva larvae hot water extract was prepared. In detail, the white spotted flower larvae obtained from Osangkensec Co., Ltd. were fasted for 3 days and then washed cleanly. 1 L of distilled water was added to 100 g of white spotted flower larvae, which had been washed, and hot water was extracted at 100° C. for 12 hours. Thereafter, impurities were removed by filtration, and centrifugation was performed at a rate of 1000 rpm to recover the supernatant, thereby producing a white-spotted flower larvae hot water extract.

Subsequently, a spore suspension was prepared using the white spotted flower larva larvae hot water extract. The spore suspension was prepared in the same process as in Example <2-2>, except that instead of'mineral water','white spotted flower radish larvae hot water extract' was used.

<2-4> Rice Grasshopper Hot water extract Preparation of used spore suspension

First, a rice locust hot water extract was prepared. In detail, 1 L of distilled water was added to 100 g of dried rice locust purchased from Dongui Bio Co., Ltd., to extract hot water at 100° C. for 12 hours. Thereafter, impurities were removed by filtration, and centrifugation was performed at a rate of 1000 rpm to recover the supernatant, thereby preparing a rice locust hot water extract.

Thereafter, a spore suspension was prepared using the prepared rice locust hot water extract. The spore suspension was prepared in the same manner as in Example <2-2>, except that instead of'mineral water','rice locust hot water extract' was used.

<2-5> pine needles Hot water extract Preparation of used spore suspension

First, a pine needle hot water extract was prepared. In detail, pine needles were collected, washed in running water, air-dried, and finely ground to prepare a pine needle powder. After adding 1 L of distilled water to 20 g of pine needle powder, hot water was extracted for 12 hours, and the extract was filtered to prepare a pine needle extract.

Thereafter, a spore suspension was prepared using the prepared pine needle hot water extract. The spore suspension was prepared in the same manner as in Example <2-2> except that'pine needle extract' was used instead of'mineral water'.

< Example 3>

Changes in Codysepin Content by Culture Conditions of Longevity Scarab Pupa Cordyceps

In the present invention, in order to establish optimum conditions for mycelial culture and fruiting body culture after injection inoculation by spore suspension type in a host, a change in the codycepin content according to temperature conditions and the number of days of culture was examined.

Briefly, the spore suspension prepared in Example <2-1> above was injected into 200 μl of a live beetle pupae (back part). At this time, the inoculation concentration was 10 ⁷ CFU/mL, and 60 animals were inoculated for each experimental group.

<3-1> Mycelial culture conditions

After the spore suspension was injected into the host, the mycelial culture was performed. The mycelial culture was unified with dark conditions and humidity of 60 to 70%, and the temperature condition was measured for codycepin content by culture day when cultured at 20°C, 25°C, 30°C, and 35°C, respectively.

In order to measure the codyspin content, the sample was dried in an oven at 50-65° C. until the moisture content reached 6-8%. The dried fruiting bodies were pulverized to a size of 0.5 to 1 mm with a grinder (Retsch, Haam, Germany). 5 g of dry powder was extracted with 250 ml of methanol for 22 hours, and the extract was centrifuged for 5 minutes at 1500 rpm and filtered through a 0.45 μm filter. The thus obtained extract was separated by using column adsorption chromatography (adsorption resin Diaion HP20), and codycepin was separated, and the content was measured using HPLC (Waters 600 Q-pump, 996 photodiode array detector and Water Empower software).

As a result, as shown in Table 1 and FIGS. 3A to 3C, it was confirmed that the codyspin content in the hyphae culture step was significantly increased at a temperature condition of 30° C., and particularly showed the highest codysepin content on the 45th day. . On the other hand, when cultured at a temperature of 35°C, the mycelial culture was not performed, and thus the codicepine content could not be measured.

Mycelial culture temperature and codyspin content by culture days (μg Cor/g) 20℃ 25℃ 30℃ Day 5 385 Day 5 1303 Day 5 3036 Day 10 526 Day 10 1883 Day 10 2415 Day 15 1062 Day 15 2532 Day 15 5012 Day 20 1285 Day 20 2424 Day 20 5035 Day 25 635 Day 25 1834 Day 25 8765 Day 30 527 Day 30 1692 Day 30 7384 Day 35 435 Day 35 1902 Day 35 7913 Day 40 735 Day 40 2640 Day 40 7349 Day 45 1480 Day 45 3035 Day 45 10053 Day 50 1106 Day 50 2750 Day 50 8065

<3-2> Fruiting Conditions

After the spore suspension was inoculated through Example <3-1>, the highest codycepin content was confirmed on day 45 of mycelial culture, and in this experiment, it was moved to the growth room for fruiting induction from day 45 of mycelial culture.

Fruiting conditions were unified with light conditions and humidity of 85 to 90% or higher, and temperature conditions were measured for codycepin content by culture day when cultured at 20°C, 25°C, and 30°C, respectively. Codycepin content was measured in the same manner as in <Example 3-1>.

As a result, as shown in Table 2 and FIGS. 4A to 4C, it was confirmed that the codycepin content in the fruiting body culture step was most increased at a temperature of 25° C., and particularly high codycepin from day 15 to day 45 The content was shown.

Fruiting body growth temperature and codycepin content by culture days (μg Cor/g) 20℃ 25℃ 30℃ Day 0 10433 Day 0 10433 Day 0 10433 Day 5 10877 Day 5 12554 Day 5 10546 Day 10 8066 Day 10 12007 Day 10 11572 Day 15 7305 Day 15 17031 Day 15 14453 Day 20 9984 Day 20 17421 Day 20 13844 Day 25 11056 Day 25 17240 Day 25 14583 Day 30 12568 Day 30 16504 Day 30 15384 Day 35 10861 Day 35 18084 Day 35 16084 Day 40 10861 Day 40 18148 Day 40 17008 Day 45 11861 Day 45 18883 Day 45 17021 Day 50 8510 Day 50 12205 Day 50 13520

< Example 3>

In the host Inoculation of spore suspension injection and codycepin content according to spore suspension type

In the present invention, each of the spore suspensions (mineral water, white spotted flower larvae hot water extract, rice locust hot water extract, pine needle hot water extract) prepared in <Example 2> was injected into 200 μl in a live longevity scarab pupae. At this time, the inoculation concentration was 10 ⁷ CFU/mL, and 60 animals were inoculated for each experimental group.

After inoculation by spore suspension type by host, the cells were incubated for 45 days under conditions of dark conditions, humidity of 60-70% and 30℃, and then fruiting bodies were cultured for 45 days under conditions of light and humidity of 85-90% and 25℃. The codycepin content of the fruiting body produced by the above process was measured.

As a result, as shown in Table 3 and FIG. 5, the treatment group 2 (white spotted flower larvae larvae hot water extract) showed significantly better codicepine content compared to other experimental groups.

Codycepin content of cordyceps grown by type of spore suspension injected into host Spore suspension type Codysepin content (μg Cor/g) Experiment group 1
(Mineral water) 18581 Experiment group 2
(White spotted flower larva larvae hot water extract) 28338 Experiment group 3
(Rice locust hot water extract) 20445 Experiment group 4
(Pine needle hot water extract) 17311 Control
(Sterile water) 18883

So far, the present invention has been focused on the preferred embodiments. Those skilled in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered in terms of explanation, not limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent range should be interpreted as being included in the present invention.

Claims (8)

A spore suspension containing the spore and insect extract of Cordyceps sinensis as an active ingredient. According to claim 1,
The cordyceps strain is Cordyceps Spore suspension, characterized in that militaris ). According to claim 1,
The insect extract is a spore suspension, characterized in that the hot water extract or rice locust hot water extract of white spotted flower larvae. According to claim 1,
The spore suspension of the Cordyceps sinensis is characterized in that the spore suspension is contained in 10 ⁶ to 10 ⁷ CFU / mL. (a) inoculating a living beetle with the spore suspension of claim 1; And
(b) comprising the step of incubating the injection-inoculated longevity scarab for 25 to 45 days at 60 to 70% humidity and 26 to 30°C temperature conditions, a method for preparing cordyceps cordyceps with enhanced codysepine content. The method of claim 5,
After the step (b) (c) further comprising the step of culturing the mycelium cultured longevity beetle at 85-95% humidity and 20 ℃ ~ 30 ℃ temperature for 15 to 45 days fruiting body, longevity enhanced codyspin content How to make scarab cordyceps. The longevity scarab cordyceps that has been enhanced by the codycepin content produced by the method of claim 6. The method of claim 7,
The longevity scarab cordyceps is longevity scarab cordyceps which contains codycepin in an amount of 10 to 30 mg/g.

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