CN106495806A - A kind of implantation methods of Medium for Ganoderma lucidum, preparation method and glossy ganoderma - Google Patents
A kind of implantation methods of Medium for Ganoderma lucidum, preparation method and glossy ganoderma Download PDFInfo
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- CN106495806A CN106495806A CN201610968831.7A CN201610968831A CN106495806A CN 106495806 A CN106495806 A CN 106495806A CN 201610968831 A CN201610968831 A CN 201610968831A CN 106495806 A CN106495806 A CN 106495806A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses the implantation methods of a kind of Medium for Ganoderma lucidum and preparation method thereof and glossy ganoderma, the culture medium is made up of the following raw material according to mass percent meter：Corn ear 50%～60%, weed tree sawdust 30%～40%, and the mixture 10%～15% of wheat skin, corn flour, land plaster and brown sugar, the content of wherein wheat skin, corn flour, land plaster and brown sugar is all higher than 0, proportioning mixing is carried out according to above ratio, and mixed material is loaded in the polypropylene or polythene film bag of 17 centimetres of * 33 centimetres of * 0.05 centimetre of specifications, load the mixed material of 0.5～1.5kg in each bag film, according to the plantation that the cultured culture medium carries out glossy ganoderma.The ganoderma lucidum content that the inventive method production is obtained is high, and production capacity is larger.
Description
Technical field
Fungus growing technique field of the present invention, especially the plantation side of a kind of Medium for Ganoderma lucidum, preparation method and glossy ganoderma
Method.
Background technology
Glossy ganoderma is the famous and precious kind in medicinal and edible health fungi, is sealed as celestial grass since ancient times.Modern medicine confirmation,
Take glossy ganoderma and can improve immunity of organisms, reach the effect that prevents and cures diseases.For meeting wilderness demand of the people to glossy ganoderma, modern big
Amount culture glossy ganoderma by the way of artificial.The compost of prior art culture glossy ganoderma mainly has linden, wood chip, cotton seed hulls to be equipped with wheat
Bran, analysis for soybean powder, land plaster etc..Use linden.Wood chip culture glossy ganoderma will consume the forest reserves in a large number；Although with cotton seed hulls culture glossy ganoderma
It is the recycling of agricultural byproducts, but cotton seed hulls there are residues of pesticides, which utilizes and is subject to certain restrictions.Additionally, Medium for Ganoderma lucidum
Build environment require higher, need one to pollute the culture that extremely low Environment space can just smoothly complete culture medium.
Content of the invention
The technical problem to be solved in the present invention is to provide the implantation methods of a kind of Medium for Ganoderma lucidum, preparation method and glossy ganoderma,
And the glossy ganoderma implantation methods based on the culture medium, plant that the ganoderma lucidum content that obtains is higher, yield is bigger.
In order to solve above-mentioned technical problem, the present invention takes technical scheme below：
A kind of Medium for Ganoderma lucidum, the culture medium are made up of the following raw material according to mass percent meter：Corn ear 50%
～60%, weed tree sawdust 30%～40%, and the mixture 10%～15% of wheat skin, corn flour, land plaster and brown sugar.
The content of the wheat skin, corn flour, land plaster and brown sugar is all higher than 0.
The culture medium is made up of the following raw material according to mass percent meter：Corn ear 50%, weed tree sawdust 40%, and
The mixture 10% of wheat skin, corn flour, land plaster and brown sugar.
A kind of preparation method of Medium for Ganoderma lucidum, comprises the following steps：
The mixture of corn ear, weed tree sawdust and wheat skin, corn flour, land plaster and brown sugar is mixed according to ratio
Close uniformly, then carry out crushing and form powder and be uniformly mixing to obtain mixed dry material；
Pack, mixed dry material is loaded in film poly-bag, and the sack of the film poly-bag is tightened, and in the bag fills in cotton
Flower；
Carry out disinfection sterilization treatment to the film poly-bag for installing mixed dry material.
The polypropylene or polythene film bag of the poly-bag preferably 17 centimetres of * 33 centimetres of * 0.05 centimetre of specifications, wherein 17 lis
Width of the rice for the poly-bag, 33 centimetres of height for the poly-bag, 0.05 centimetre of thickness for the poly-bag.
The quality of the mixed dry material loaded in the poly-bag is 0.5～1.5kg.
The sack of the poly-bag is tightened using rope or lasso banding.
In the sterilization processing procedure, the poly-bag for installing mixed dry material is put in atmospheric sterilizing stove, 100 DEG C with
On temperature under kept for 20～25 hours, then vexed stove 4～6 hours naturally, complete the preparation of culture medium.
A kind of implantation methods of base glossy ganoderma, comprise the following steps：
Inoculation, culture medium is put to aseptic inoculation room, Ganoderma lucidum mycelium is seeded on culture medium；
Bacterium is sent out, postvaccinal culture medium is emitted on the indoor bedstead of culture, culture medium is completely covered in Ganoderma lucidum mycelium
In stage before charge level, indoor temperature control is cultivated at 22～25 DEG C；When Ganoderma lucidum mycelium be completely covered seal culture charge level it
Afterwards, by temperature control indoor for culture at 25～28 DEG C, humid control is 60%～70%；
When there is overshooting shape former base and swell 1.5～1.6 centimetres to poly-bag sack, and the Ganoderma Lucidum of culture medium charge level
When the Ganoderma lucidum mycelium portions turn color and fibrillatable of the bag wall of silk and poly-bag, poly-bag bag opening part is cut off, retain diameter
For 2～3 centimetres of passage, now glossy ganoderma sends out bacterium and completes, and the Medium for Ganoderma lucidum for sending out well bacterium is moved into cultivation in booth；
During the Medium for Ganoderma lucidum that bacterium is sent out in cultivation good in the booth, trench digging is carried out to cultivation area first and does furrow and sterilization, so
Afterwards Medium for Ganoderma lucidum is put in ditch and covers soil；
When growing in the booth, the light brown of the white when the cap of glossy ganoderma is by initial growth to growth phase and growth
During the brown for completing, the air humidity in booth is kept 85～95%；
Go out the sesame stage in glossy ganoderma, keep the temperature in booth at 25～28 DEG C, humidity 90～95% often uses day ultrasonic fog
Change humidifier humidifies 4～6 times, each humidification periods are 30 minutes, complete the plantation of glossy ganoderma.
During described bacterium, when the temperature of culturing room is higher than 22 DEG C, daily to cultivating chamber ventilated twice, divulge information every time
Time is 10～12 hours；When the temperature of culturing room is less than 22 DEG C, every other day to cultivating chamber ventilated once, ventilation time is
2～3 hours.
The glossy ganoderma that the inventive method is obtained, nutrient content are higher, and yield is bigger.
Specific embodiment
For the ease of the understanding of those skilled in the art, the present invention is further retouched with reference to specific embodiment
State.
Embodiment one
Step 1, according to the raw material that mass percent selects following ratio：Corn ear 50%, weed tree sawdust 40%, Yi Jimai
The mixture 10% of skin, corn flour, land plaster and brown sugar.The content of wheat skin, corn flour, land plaster and brown sugar is all higher than 0, and
Ensure that the content of wheat skin, corn flour, land plaster and brown sugar flexibly arbitrarily can be selected in the case where summation is 10%.Will
The raw material of said ratio is well mixed, and then carries out crushing and forms powder and be uniformly mixing to obtain mixed dry material.
Step 2, pack load mixed dry material in film poly-bag, and the sack of the film poly-bag is tightened, and in the bag
Fill in cotton.The polypropylene or polythene film bag of poly-bag preferably 17 centimetres of * 33 centimetres of * 0.05 centimetre of specifications, wherein 17 centimetres
For the width of the poly-bag, 33 centimetres of height for the poly-bag, 0.05 centimetre of thickness for the poly-bag.The mixing loaded in poly-bag is done
The quality of material is 0.5kg.Wherein, the sack of poly-bag is tightened using rope or lasso banding, convenient operation.
Step 3, carry out disinfection sterilization treatment to the poly-bag for installing mixed dry material.The poly-bag for installing mixed dry material is put into often
In pressure sterilizing kitchen, 23 hours are kept at a temperature of 100 DEG C, then vexed stove 6 hours naturally, complete the preparation of culture medium, obtain
Medium for Ganoderma lucidum.
The Medium for Ganoderma lucidum obtained through above-mentioned culture is carried out Ganoderma Lucidum as matrix by a kind of implantation methods of base glossy ganoderma
The growth that plants, specifically includes following steps：
S1, inoculation, culture medium is put to aseptic inoculation room, Ganoderma lucidum mycelium is seeded on culture medium.Generally will culture
Base maintains less than 30 DEG C of temperature and carries out inoculation operation.
S2, sends out bacterium, postvaccinal culture medium is emitted on the indoor bedstead of culture, culture is completely covered in Ganoderma lucidum mycelium
In stage before base-material face, indoor temperature control is cultivated at 22 DEG C, it is to avoid Ganoderma lucidum mycelium is dirty by miscellaneous bacteria at a higher temperature
Dye；After Ganoderma lucidum mycelium is completely covered and seals culture charge level, the indoor temperature of culture is promoted to 25 DEG C from 22 DEG C before,
Humid control is 60%.When the temperature of culturing room is higher than 22 DEG C, daily to cultivating chamber ventilated twice, each ventilation time is 10
Hour；When the temperature of culturing room is less than 22 DEG C, every other day to cultivating chamber ventilated once, ventilation time is 2 hours.In ventilation
During, in addition it is also necessary to the big ups and downs of temperature are avoided, the low photoenvironment to culturing room is kept.For putting for culture medium close
Degree needs to leave suitable space, it is ensured that the circulation and the growth of mycelia of air.After being generally inoculated with 10 days, Ganoderma lucidum mycelium can be walked
The charge level of full Medium for Ganoderma lucidum, and deeply grow in the material of the Medium for Ganoderma lucidum, fruit body primordium can be formed within 30～35 days.
S3, after Ganoderma lucidum mycelium walks full culture medium charge level, it may appear that white projection, when the white overshooting shape former base and
1.52 centimetres are swelled to poly-bag sack, and the Ganoderma lucidum mycelium portions turn of the bag wall of the Ganoderma lucidum mycelium and poly-bag of culture medium charge level
When color and fibrillatable, poly-bag bag opening part is cut off, retains a diameter of 2 centimetres of passage, now glossy ganoderma sends out bacterium and completes,
The Medium for Ganoderma lucidum for sending out bacterium good is moved in booth and is cultivated.
S4, during the Medium for Ganoderma lucidum that bacterium is sent out in cultivation good in the booth, first carries out trench digging to cultivation area and does furrow and sterilization,
Then Medium for Ganoderma lucidum is put in ditch and covers soil.During sterilization, can be carried out disinfection with water dissolves using potassium permanganate, or
After person other disinfectant water dissolves, sterilization is realized in sprinkling.
For booth place place, need to ensure that place surrounding environment is substantially free of contaminants.Water quality reaches requirement, especially
In water, heavy metal or agricultural chemicals can not all play standard.
S5, when growing in the booth, the light brown of the white when the cap of glossy ganoderma is by initial growth to growth phase and
During the brown that completes of growth, air humidity in booth is kept 85%.Medium for Ganoderma lucidum is buried after soil, needs to carry out
Moisturizing and ventilation, and avoid sunlight from shining directly on culture medium.According to sesame density is gone out, can suitably remove some connecting handles
Sesame, mutually extrudes after being grown up with bacteria-proof cover, affects quality.Generally stem grows cap after the growth of 13 days successively, the bacterium
The color of lid progressively switchs to light brown by white, and then deepens into brown.Temperature control in canopy is at 25 DEG C.
S6, goes out the sesame stage in glossy ganoderma, keeps the temperature in booth at 25 DEG C, and humidity 90% is often added with day ultrasonic atomization
Wet device is humidified 4 times, and each humidification periods are 30 minutes, complete the plantation of glossy ganoderma.
Then ganoderma lucidum fruitbody Later growth will enter the spores release stage, and in good time carries out bagging to cap.The set
During the bag time should select the white edge of ganoderma lucidum fruitbody to be wholly absent afterwards, bag made of paper is generally used, prevents conidia powder outside
Disperse.Now indoor temperature is normally controlled in 24 DEG C, air humidity 85%, and in good time ventilation, keeps the new of room air
Fresh, prevent gas concentration lwevel from increasing.
Embodiment two
Step 1, according to the raw material that mass percent selects following ratio：Corn ear 55%, weed tree sawdust 35%, Yi Jimai
The mixture 10% of skin, corn flour, land plaster and brown sugar.The content of wheat skin, corn flour, land plaster and brown sugar is all higher than 0, and
Ensure that the content of wheat skin, corn flour, land plaster and brown sugar flexibly arbitrarily can be selected in the case where summation is 10%.Will
The raw material of said ratio is well mixed, and then carries out crushing and forms powder and be uniformly mixing to obtain mixed dry material.
Step 2, pack load mixed dry material in film poly-bag, and the sack of the film poly-bag is tightened, and in the bag
Fill in cotton.The polypropylene or polythene film bag of poly-bag preferably 17 centimetres of * 33 centimetres of * 0.05 centimetre of specifications, wherein 17 centimetres
For the width of the poly-bag, 33 centimetres of height for the poly-bag, 0.05 centimetre of thickness for the poly-bag.The mixing loaded in poly-bag is done
The quality of material is 1kg.Wherein, the sack of poly-bag is tightened using rope or lasso banding, convenient operation.
Step 3, carry out disinfection sterilization treatment to the poly-bag for installing mixed dry material.The poly-bag for installing mixed dry material is put into often
In pressure sterilizing kitchen, 22 hours are kept at a temperature of 110 DEG C, then vexed stove 5 hours naturally, complete the preparation of culture medium, obtain
Medium for Ganoderma lucidum.
The Medium for Ganoderma lucidum obtained through above-mentioned culture is carried out Ganoderma Lucidum as matrix by a kind of implantation methods of base glossy ganoderma
The growth that plants, specifically includes following steps：
S1, inoculation, culture medium is put to aseptic inoculation room, Ganoderma lucidum mycelium is seeded on culture medium.Generally will culture
Base maintains less than 30 DEG C of temperature and carries out inoculation operation.
S2, sends out bacterium, postvaccinal culture medium is emitted on the indoor bedstead of culture, culture is completely covered in Ganoderma lucidum mycelium
In stage before base-material face, indoor temperature control is cultivated at 24 DEG C, it is to avoid Ganoderma lucidum mycelium is dirty by miscellaneous bacteria at a higher temperature
Dye；After Ganoderma lucidum mycelium is completely covered and seals culture charge level, the indoor temperature of culture is promoted to 27 DEG C from 24 DEG C before,
Humid control is 65%.When the temperature of culturing room is higher than 22 DEG C, daily to cultivating chamber ventilated twice, each ventilation time is 11
Hour；When the temperature of culturing room is less than 22 DEG C, every other day to cultivating chamber ventilated once, ventilation time is 2 hours.In ventilation
During, in addition it is also necessary to the big ups and downs of temperature are avoided, the low photoenvironment to culturing room is kept.For putting for culture medium close
Degree needs to leave suitable space, it is ensured that the circulation and the growth of mycelia of air.After being generally inoculated with 10 days, Ganoderma lucidum mycelium can be walked
The charge level of full Medium for Ganoderma lucidum, and deeply grow in the material of the Medium for Ganoderma lucidum, fruit body primordium can be formed within 30～35 days.
S3, after Ganoderma lucidum mycelium walks full culture medium charge level, it may appear that white projection, when the white overshooting shape former base and
1.55 centimetres are swelled to poly-bag sack, and the Ganoderma lucidum mycelium portions turn of the bag wall of the Ganoderma lucidum mycelium and poly-bag of culture medium charge level
When color and fibrillatable, poly-bag bag opening part is cut off, retains a diameter of 3 centimetres of passage, now glossy ganoderma sends out bacterium and completes,
The Medium for Ganoderma lucidum for sending out bacterium good is moved in booth and is cultivated.
S4, during the Medium for Ganoderma lucidum that bacterium is sent out in cultivation good in the booth, first carries out trench digging to cultivation area and does furrow and sterilization,
Then Medium for Ganoderma lucidum is put in ditch and covers soil.During sterilization, can be carried out disinfection with water dissolves using potassium permanganate, or
After person other disinfectant water dissolves, sterilization is realized in sprinkling.
For booth place place, need to ensure that place surrounding environment is substantially free of contaminants.Water quality reaches requirement, especially
In water, heavy metal or agricultural chemicals can not all play standard.
S5, when growing in the booth, the light brown of the white when the cap of glossy ganoderma is by initial growth to growth phase and
During the brown that completes of growth, air humidity in booth is kept 90%.Medium for Ganoderma lucidum is buried after soil, needs to carry out
Moisturizing and ventilation, and avoid sunlight from shining directly on culture medium.According to sesame density is gone out, can suitably remove some connecting handles
Sesame, mutually extrudes after being grown up with bacteria-proof cover, affects quality.Generally stem grows cap after the growth of 13 days successively, the bacterium
The color of lid progressively switchs to light brown by white, and then deepens into brown.Temperature control in canopy is at 28 DEG C.
S6, goes out the sesame stage in glossy ganoderma, keeps the temperature in booth at 27 DEG C, and humidity 93% is often added with day ultrasonic atomization
Wet device is humidified 5 times, and each humidification periods are 30 minutes, complete the plantation of glossy ganoderma.
Then ganoderma lucidum fruitbody Later growth will enter the spores release stage, and in good time carries out bagging to cap.The set
During the bag time should select the white edge of ganoderma lucidum fruitbody to be wholly absent afterwards, bag made of paper is generally used, prevents conidia powder outside
Disperse.Now indoor temperature is normally controlled in 24 DEG C, air humidity 85%, and in good time ventilation, keeps the new of room air
Fresh, prevent gas concentration lwevel from increasing.
After glossy ganoderma full maturity, it is possible to carry out harvesting work.Generally forming harvesting from cap about needs 20 days, lid bacterium
The color at lid edge switchs to brown by white, when the conidia powder of coffee color is indistinctly seen at the cap back side by harvested.First
Criticize after harvesting is finished and sterilized once, be separated by 7 days and spray water afterwards again, make Ganoderma lucidum mycelium restoration ecosystem, promote new Ganoderma Lucidum
Flower bud is formed, and is generally separated by the harvesting that can achieve second batch glossy ganoderma for 2 months.
Embodiment three
Step 1, according to the raw material that mass percent selects following ratio：Corn ear 60%, weed tree sawdust 30%, Yi Jimai
The mixture 10% of skin, corn flour, land plaster and brown sugar.The content of wheat skin, corn flour, land plaster and brown sugar is all higher than 0, and
Ensure that the content of wheat skin, corn flour, land plaster and brown sugar flexibly arbitrarily can be selected in the case where summation is 10%.Will
The raw material of said ratio is well mixed, and then carries out crushing and forms powder and be uniformly mixing to obtain mixed dry material.
Step 2, pack load mixed dry material in film poly-bag, and the sack of the film poly-bag is tightened, and in the bag
Fill in cotton.The polypropylene or polythene film bag of poly-bag preferably 17 centimetres of * 33 centimetres of * 0.05 centimetre of specifications, wherein 17 centimetres
For the width of the poly-bag, 33 centimetres of height for the poly-bag, 0.05 centimetre of thickness for the poly-bag.The mixing loaded in poly-bag is done
The quality of material is 1.5kg.Wherein, the sack of poly-bag is tightened using rope or lasso banding, convenient operation.
Step 3, carry out disinfection sterilization treatment to the poly-bag for installing mixed dry material.The poly-bag for installing mixed dry material is put into often
In pressure sterilizing kitchen, 20 hours are kept at a temperature of 120 DEG C, then vexed stove 4 hours naturally, complete the preparation of culture medium, obtain
Medium for Ganoderma lucidum.
The Medium for Ganoderma lucidum obtained through above-mentioned culture is carried out Ganoderma Lucidum as matrix by a kind of implantation methods of base glossy ganoderma
The growth that plants, specifically includes following steps：
S1, inoculation, culture medium is put to aseptic inoculation room, Ganoderma lucidum mycelium is seeded on culture medium.Generally will culture
Base maintains less than 30 DEG C of temperature and carries out inoculation operation.
S2, sends out bacterium, postvaccinal culture medium is emitted on the indoor bedstead of culture, culture is completely covered in Ganoderma lucidum mycelium
In stage before base-material face, indoor temperature control is cultivated at 25 DEG C, it is to avoid Ganoderma lucidum mycelium is dirty by miscellaneous bacteria at a higher temperature
Dye；After Ganoderma lucidum mycelium is completely covered and seals culture charge level, the indoor temperature of culture is promoted to 28 DEG C from 25 DEG C before,
Humid control is 70%.When the temperature of culturing room is higher than 22 DEG C, daily to cultivating chamber ventilated twice, each ventilation time is 12
Hour；When the temperature of culturing room is less than 22 DEG C, every other day to cultivating chamber ventilated once, ventilation time is 2 hours.In ventilation
During, in addition it is also necessary to the big ups and downs of temperature are avoided, the low photoenvironment to culturing room is kept.For putting for culture medium close
Degree needs to leave suitable space, it is ensured that the circulation and the growth of mycelia of air.After being generally inoculated with 10 days, Ganoderma lucidum mycelium can be walked
The charge level of full Medium for Ganoderma lucidum, and deeply grow in the material of the Medium for Ganoderma lucidum, fruit body primordium can be formed within 30～35 days.
S3, after Ganoderma lucidum mycelium walks full culture medium charge level, it may appear that white projection, when the white overshooting shape former base and
1.6 centimetres are swelled to poly-bag sack, and the Ganoderma lucidum mycelium portions turn of the bag wall of the Ganoderma lucidum mycelium and poly-bag of culture medium charge level
When color and fibrillatable, poly-bag bag opening part is cut off, retains a diameter of 2 centimetres of passage, now glossy ganoderma sends out bacterium and completes,
The Medium for Ganoderma lucidum for sending out bacterium good is moved in booth and is cultivated.
S4, during the Medium for Ganoderma lucidum that bacterium is sent out in cultivation good in the booth, first carries out trench digging to cultivation area and does furrow and sterilization,
Then Medium for Ganoderma lucidum is put in ditch and covers soil.During sterilization, can be carried out disinfection with water dissolves using potassium permanganate, or
After person other disinfectant water dissolves, sterilization is realized in sprinkling.
For booth place place, need to ensure that place surrounding environment is substantially free of contaminants.Water quality reaches requirement, especially
In water, heavy metal or agricultural chemicals can not all play standard.
S5, when growing in the booth, the light brown of the white when the cap of glossy ganoderma is by initial growth to growth phase and
During the brown that completes of growth, air humidity in booth is kept 85%.Medium for Ganoderma lucidum is buried after soil, needs to carry out
Moisturizing and ventilation, and avoid sunlight from shining directly on culture medium.According to sesame density is gone out, can suitably remove some connecting handles
Sesame, mutually extrudes after being grown up with bacteria-proof cover, affects quality.Generally stem grows cap after the growth of 13 days successively, the bacterium
The color of lid progressively switchs to light brown by white, and then deepens into brown.Temperature control in canopy is at 25 DEG C.
S6, goes out the sesame stage in glossy ganoderma, keeps the temperature in booth at 28 DEG C, and humidity 95% is often added with day ultrasonic atomization
Wet device is humidified 6 times, and each humidification periods are 30 minutes, complete the plantation of glossy ganoderma.
Then ganoderma lucidum fruitbody Later growth will enter the spores release stage, and in good time carries out bagging to cap.The set
During the bag time should select the white edge of ganoderma lucidum fruitbody to be wholly absent afterwards, bag made of paper is generally used, prevents conidia powder outside
Disperse.Now indoor temperature is normally controlled in 24 DEG C, air humidity 85%, and in good time ventilation, keeps the new of room air
Fresh, prevent gas concentration lwevel from increasing.
It should be noted that the above is not limited to the present invention, in the creation design without departing from the present invention
Under the premise of, any obvious replacement is within protection scope of the present invention.
Claims (10)
1. a kind of Medium for Ganoderma lucidum, it is characterised in that the culture medium is made up of the following raw material according to mass percent meter：Beautiful
Rice rod 50%～60%, the mixture 10% of weed tree sawdust 30%～40% and wheat skin, corn flour, land plaster and brown sugar～
15%.
2. Medium for Ganoderma lucidum according to claim 1, it is characterised in that the wheat skin, corn flour, land plaster and brown sugar
Content is all higher than 0.
3. Medium for Ganoderma lucidum according to claim 2, it is characterised in that the culture medium is by according to mass percent meter
Following raw material is constituted：The mixture 10% of corn ear 50%, weed tree sawdust 40%, and wheat skin, corn flour, land plaster and brown sugar.
4. a kind of preparation method of the Medium for Ganoderma lucidum according to claim 1 or 2 or 3, it is characterised in that including following step
Suddenly：
The mixture of corn ear, weed tree sawdust and wheat skin, corn flour, land plaster and brown sugar is carried out mixing according to ratio
Even, then carry out crushing and form powder and be uniformly mixing to obtain mixed dry material；
Pack, mixed dry material is loaded in film poly-bag, and the sack of the film poly-bag is tightened, and in the bag fills in cotton；
Carry out disinfection sterilization treatment to the film poly-bag for installing mixed dry material.
5. the preparation method of Medium for Ganoderma lucidum according to claim 4, it is characterised in that preferably 17 centimetres * of the poly-bag
The polypropylene or polythene film bag of 33 centimetres of * 0.05 centimetre of specifications, the wherein 17 centimetres width for the poly-bag, 33 centimetres is to be somebody's turn to do
The height of poly-bag, 0.05 centimetre of thickness for the poly-bag.
6. the preparation method of Medium for Ganoderma lucidum according to claim 5, it is characterised in that the mixing loaded in the poly-bag
The quality of siccative is 0.5～1.5kg.
7. the preparation method of Medium for Ganoderma lucidum according to claim 6, it is characterised in that the sack of the poly-bag is using rope
Son is tightened or lasso banding.
8. the preparation method of Medium for Ganoderma lucidum according to claim 7, it is characterised in that the sterilization processing procedure
In, the poly-bag for installing mixed dry material is put in atmospheric sterilizing stove, holding 20～25 hours at the temperature more than 100 DEG C, so
Naturally vexed stove 4～6 hours, complete the preparation of culture medium afterwards.
9. a kind of implantation methods of the glossy ganoderma of the Medium for Ganoderma lucidum based on according to any one of claim 1～8, including with
Lower step：
Inoculation, culture medium is put to aseptic inoculation room, Ganoderma lucidum mycelium is seeded on culture medium；
Bacterium is sent out, postvaccinal culture medium is emitted on the indoor bedstead of culture, culture medium charge level is completely covered in Ganoderma lucidum mycelium
In the stage before, indoor temperature control is cultivated at 22～25 DEG C；After Ganoderma lucidum mycelium is completely covered and seals culture charge level, will
At 25～28 DEG C, humid control is 60%～70% for the indoor temperature control of culture；
When occur overshooting shape former base and to poly-bag sack swell 1.5～1.6 centimetres, and the Ganoderma lucidum mycelium of culture medium charge level and
When the Ganoderma lucidum mycelium portions turn color and fibrillatable of the bag wall of poly-bag, poly-bag bag opening part is cut off, retain a diameter of 2～
3 centimetres of passage, now glossy ganoderma send out bacterium and complete, the Medium for Ganoderma lucidum for sending out well bacterium is moved into cultivation in booth；
During the Medium for Ganoderma lucidum that bacterium is sent out well in cultivation in the booth, trench digging is carried out to cultivation area first and does furrow and sterilization, then general
Medium for Ganoderma lucidum is put in ditch and covers soil；
When growing in the booth, the light brown of the white when the cap of glossy ganoderma is by initial growth to growth phase and growing is completed
Brown during, keep the air humidity in booth 85～95%；
Go out the sesame stage in glossy ganoderma, keep the temperature in booth at 25～28 DEG C, humidity 90～95% is often added with day ultrasonic atomization
Wet device is humidified 4～6 times, and each humidification periods are 30 minutes, complete the plantation of glossy ganoderma.
10. implantation methods of glossy ganoderma according to claim 9, it is characterised in that during described bacterium, when culturing room
When temperature is higher than 22 DEG C, daily to cultivating chamber ventilated twice, each ventilation time is 10～12 hours；When the temperature of culturing room low
When 22 DEG C, every other day to cultivating chamber ventilated once, ventilation time is 2～3 hours.
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| CN109275491A (en) * | 2018-05-19 | 2019-01-29 | 宝鸡胜利现代农业开发有限公司 | A kind of ganoderma lucidum production technology |
| CN112493054A (en) * | 2020-11-23 | 2021-03-16 | 安徽农业大学 | Ganoderma lucidum culture material based on moso bamboo sawdust and preparation method and application thereof |
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