CN108587924B - Ganoderma lucidum mycelium pellet product - Google Patents
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Abstract
A preparation method of a ganoderma lucidum mycelium pellet product comprises the following steps: (1) preparing a culture solution; (2) treating the ganoderma lucidum seed liquid; (3) culturing: taking a culture solution, adding 2-4mL of hypha suspension into every 1L of the culture solution, transferring the hypha suspension into the culture solution, carrying out vortex oscillation for 20-30 min, then carrying out culture for 1-2 days at 26-28 ℃ and 160-200 r/min, standing for 2-4 h at the same temperature, and then carrying out culture for 2-3 days at 26-28 ℃ and 60-100 r/min to obtain the ganoderma lucidum mycelium pellets. The invention develops the colorful ganoderma lucidum mycelium pellet product which is rich in ornamental value, pure natural, recyclable and environment-friendly to replace the existing synthetic expansible crystal pellet product, widens the application value and the commercial value of the traditional Chinese medicine ganoderma lucidum and ganoderma lucidum liquid mycelium pellets, and is a beneficial attempt of the traditional Chinese medicine culture application industry.
Description
Technical Field
The invention relates to a ganoderma lucidum mycelium pellet product.
Background
At present, a large number of water-swelling crystal ball products are sold in the market, and the spherical crystal babies absorb water and swell, are popular with children and women, and have wide sale markets, but the products have great defects. The Shanghai organic chemistry research institute of Chinese academy detects that the main components of the product are acrylonitrile, polyacrylate and the like, and the acrylonitrile, the polyacrylate and the like have certain toxicity, so that children take the product by mistake can bring great potential safety hazard. In addition, such products do not degrade naturally and the resulting waste materials have a very negative environmental impact. On the basis of previous full investigation, the existing crystal ball handicraft has the outstanding problems of high toxicity, no degradation, high potential safety hazard and the like, and is not beneficial to the health of children.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defects of the prior art and provide a ganoderma lucidum mycelium pellet product which is nontoxic to human bodies and can be naturally degraded.
The technical scheme adopted by the invention for solving the technical problems is that the preparation method of the ganoderma lucidum mycelium pellet product comprises the following steps:
(1) preparing a culture solution, wherein the formula of the culture solution is as follows:
1.1 formula (1L calculation) for culturing yellow Ganoderma mycelium pellet: 200-220g of peeled potato, 8-10g of corn kernels and 20-22g of glucose, and adding water to 1L.
1.2 formula (1L calculation) for culturing white Ganoderma mycelium pellet: 8-10g of peptone, 8-10g of poria cocos extract, 38-40g of glucose, and adding water to 1L.
1.3 formula for culturing mycelium pellet of Ganoderma lucidum (1L calculation): 8-10g of peptone, 0.01-0.02g of beet red, 38-40g of glucose, and adding water to 1L.
1.4 Green Ganoderma mycelium pellet culture formula (1L calculation): 8-10g of peptone, 0.02-0.03g of gardenia green pigment and 38-40g of glucose, and adding water to 1L.
1.5 blue Ganoderma mycelium pellet culture formula (1L calculation): 8-10g of peptone, 0.01-0.02g of gardenia blue pigment and 38-40g of glucose, and adding water to 1L.
1.6 purple Ganoderma mycelium pellet culture formula (1L calculation): 8-10g of peptone, 0.01-0.02g of purple yam pigment, 38-40g of glucose, and adding water to 1L.
1.7 Black Ganoderma mycelium pellet culture formula (1L calculation): 8-10g of peptone, 0.01-0.02g of plant charcoal melanin and 38-40g of glucose, and adding water to 1L.
In each liquid culture formula, 0.1-0.2mol/L sodium hydroxide solution is used for regulating the pH value to 6.8-7.2;
(2) treating the ganoderma lucidum seed liquid (in order to ensure that ganoderma lucidum mycelium pellets can be directionally cultured into fixed shapes with uniform sizes): grinding Ganoderma seed solution into slurry under aseptic condition with homogenizer, transferring into glass syringe, sleeving with replaceable membrane chromatographic filter, and filtering with filter paper instead of membrane to obtain mycelium suspension.
(3) Culturing: and (3) taking the culture solution obtained in the step (1), adding 2-4mL of hypha suspension into every 1L of culture solution, transferring the hypha suspension obtained in the step (2), transferring the hypha suspension into the culture solution, carrying out vortex oscillation for 20-30 min, then, culturing for 1-2 days at 26-28 ℃ and 160-200 r/min, standing for 2-4 h at the same temperature, and then, culturing for 2-3 days at 26-28 ℃ and 60-100 r/min to obtain the ganoderma mycelium pellets.
This step is to further ensure that the final ganoderma lucidum mycelia can be directionally cultured into a spherical shape, rather than a filamentous, elliptical or other irregular shape.
In order to increase the interest of the product, the mycelium pellet can be directionally cultured into a hedgehog ball shape, and the following treatment can be carried out: preparing a chitosan solution with the concentration of 0.2-0.3g/mL by using a glacial acetic acid aqueous solution with the volume concentration of 0.5-1.5% as a solvent, and adding the chitosan solution into the culture solution in the step (1) according to the amount of adding 1-2mL of chitosan solution into every 1000mL of culture solution; the other processing method is the same as the preparation method of the ganoderma lucidum mycelium pellet product.
Ganoderma ganodermatum is a fungus of Ganoderma genus of Polyporaceae of Basidiomycetes, belongs to one of famous and precious medicinal fungi in China, and has a long clinical medication history. Research shows that the ganoderma lucidum mycelium pellet is from mycelium polymers obtained after liquid culture or fermentation of ganoderma lucidum strains, and the size of the pellet or other shapes can be close to that of common crystal pellets. Under certain culture conditions, the ganoderma lucidum mycelia can be cultured into specific spherical, burr-shaped or other various shapes by adopting a liquid fermentation technology, and different colors can be given to the ganoderma lucidum mycelia on the basis, so that the ornamental value is kept to the maximum extent. The pure natural characteristics of the ganoderma mycelium pellets not only solve the health hidden trouble caused by the mistaken taking of children, but also are extremely easy to degrade, cannot pollute the environment, and even can be used as flower fertilizer and fish feed to realize effective utilization once more while realizing the crystal pellet function.
The invention develops a colorful ganoderma lucidum mycelium pellet product which is rich in ornamental value, pure natural, recyclable and environment-friendly to replace the existing synthetic expansible crystal pellet product, widens the application value and the commercial value of traditional Chinese medicine ganoderma lucidum and ganoderma lucidum liquid mycelium pellets, and is a beneficial attempt of the traditional Chinese medicine culture application industry.
Drawings
FIG. 1 is a picture of a Ganoderma mycelium pellet product obtained in example 1 of the present invention;
fig. 2 is a picture of the ganoderma lucidum hedgehog ball product obtained in example 2 of the present invention.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
Example 1
The preparation method of the ganoderma lucidum mycelium pellet product comprises the following steps:
(1) preparing a culture solution, wherein the formula of the culture solution is as follows:
1.1 formula (1L calculation) for culturing yellow Ganoderma mycelium pellet: peeled potato 200g, corn kernel 10g, glucose 20g, adding water to 1L.
1.2 formula (1L calculation) for culturing white Ganoderma mycelium pellet: 10g of peptone, 10g of tuckahoe extract and 40g of glucose, and adding water to 1L.
1.3 formula for culturing mycelium pellet of Ganoderma lucidum (1L calculation): 10g of peptone, 0.01g of beet red and 40g of glucose, and adding water to 1L.
1.4 Green Ganoderma mycelium pellet culture formula (1L calculation): 10g of peptone, 0.02g of gardenia green pigment and 40g of glucose, and adding water to 1L.
1.5 blue Ganoderma mycelium pellet culture formula (1L calculation): 10g of peptone, 0.01g of gardenia blue pigment and 40g of glucose, and adding water to 1L.
1.6 purple Ganoderma mycelium pellet culture formula (1L calculation): 10g of peptone, 0.01g of purple yam pigment and 40g of glucose, and adding water to 1L.
1.7 Black Ganoderma mycelium pellet culture formula (1L calculation): 10g of peptone, 0.01g of plant charcoal melanin and 40g of glucose, and adding water to 1L.
In each liquid culture formula, 0.1mol/L sodium hydroxide solution is used for regulating the pH value to 7.0;
(2) treating the ganoderma lucidum seed liquid (in order to ensure that ganoderma lucidum mycelium pellets can be directionally cultured into fixed shapes with uniform sizes): grinding Ganoderma seed solution into slurry under aseptic condition with homogenizer, transferring into glass syringe, sleeving with replaceable membrane chromatographic filter, and filtering with a layer of filter paper instead of membrane to obtain mycelium suspension with uniform size.
(3) Culturing: and (3) taking the culture solution in the step (1), adding 2mL of hypha suspension into every 1L of culture solution, transferring the hypha suspension obtained in the step (2), transferring the hypha suspension into the culture solution, carrying out vortex oscillation for 30min, culturing for 1 day at the temperature of 28 ℃ and at the speed of 200r/min, standing for 4h at the same temperature, and culturing for 2 days at the temperature of 28 ℃ and at the speed of 100r/min to obtain the ganoderma lucidum mycelium pellets with different colors.
Example 2
Directionally culturing mycelium pellets into a hedgehog ball shape, and processing the mycelium pellets as follows: preparing a chitosan solution with the concentration of 0.2g/mL by using a glacial acetic acid aqueous solution with the volume concentration of 1% as a solvent, and adding the chitosan solution into the culture solution in the step (1) according to the amount of adding 2mL of chitosan solution into every 1000mL of culture solution; the other processing method is the same as the preparation method of the ganoderma lucidum mycelium pellet product. Thus obtaining the ganoderma lucidum hedgehog ball.
Product evaluation
1. Consumer preference evaluation
20 children (age range 8-12 years) and adolescent consumers (8 children and 12 adolescents) who purchased the hyacinths were recruited as beauty evaluators to participate in the sensory test, and the evaluators to participate in the quantitative descriptive analysis test. The consumer preference test uses nine-point hedonic scale method, 1-9 (1 represents extreme dislike, 9 represents extreme preference), and scores the color, shape and overall preference of the existing commercial crystal ball, the colorful ganoderma lucidum mycelium ball of example 1 and the colorful ganoderma lucidum mycelium hedgehog ball of example 2, respectively, and the results are shown in table 1.
As can be seen from Table 1, the color, shape and overall preference scores of the crystal ball and the colorful glossy ganoderma mycelium ball by the consumer are all above 7.2, and the scores basically have no big difference, which indicates that the colorful glossy ganoderma mycelium ball is popular with the consumer like the crystal ball; the shape and the integral favor score of the colorful glossy ganoderma hypha hedgehog ball are both higher than those of the former two, the shape score is 8.8, and the integral favor score is 8.1, which shows that the appearance of the colorful glossy ganoderma hypha hedgehog ball is more popular with consumers than crystal balls and colorful glossy ganoderma hypha balls, and has larger market application potential.
2. Evaluation of safety
The raw materials used in the production process of the colorful ganoderma lucidum mycelium pellet and the hedgehog pellet are safe, natural and free of toxicity, include various natural edible pigments, peptone, glucose, potato, chitosan and the like, are free of any synthetic raw materials, are high in safety, and cannot influence human bodies or environment after being smashed or eaten by mistake.
3. Evaluation of degradation Properties
The raw materials used in the production process of the colorful ganoderma lucidum mycelium pellet are safe, natural and easy to degrade automatically, no synthetic raw materials are introduced, and the colorful ganoderma lucidum mycelium pellet, namely the fungal microorganism, is not only easy to degrade by other microorganisms in the environment, but also can help to absorb and degrade other organic pollutants in the environment, such as plastics, rubber, organic reagents and the like, so that the environment is protected.
4. Evaluation of recyclability
In the past, ganoderma lucidum mycelia are only used as medicinal raw materials, health-care food or functional food raw materials and are mainly used in the field of large health, the ganoderma lucidum mycelia are applied to the field of children science popularization products and the field of art ornaments, the biological industrial chain of ganoderma lucidum liquid fermentation is prolonged, and the ganoderma lucidum liquid fermentation product can be effectively utilized again as flower fertilizer and fish feed after being discarded, so that the new economic development form of sustainable development and recycling is met.
Claims (2)
1. The application of the colorful ganoderma lucidum mycelium pellet product in preparing the water-swelling crystal pellet is characterized in that the preparation method of the colorful ganoderma lucidum mycelium pellet product comprises the following steps:
(1) preparing a culture solution, wherein the formula of the culture solution is as follows:
1.1 formula of culture solution of yellow ganoderma mycelium pellet: peeling potato 200-220g, corn 8-10g and glucose 20-22g, adding water to 1L;
1.2 formula of white ganoderma mycelium pellet culture solution: 8-10g of peptone, 8-10g of poria cocos extract and 38-40g of glucose, and adding water to 1L;
1.3 formula of red ganoderma mycelium pellet culture solution: 8-10g of peptone, 0.01-0.02g of beet red and 38-40g of glucose, and adding water to 1L;
1.4 formula of green ganoderma mycelium pellet culture solution: 8-10g of peptone, 0.02-0.03g of gardenia green pigment and 38-40g of glucose, and adding water to 1L;
1.5 formula of blue ganoderma mycelium pellet culture solution: 8-10g of peptone, 0.01-0.02g of gardenia blue pigment and 38-40g of glucose, and adding water to 1L;
1.6 formula of purple ganoderma mycelium pellet culture solution: 8-10g of peptone, 0.01-0.02g of purple yam pigment and 38-40g of glucose, and adding water to 1L;
1.7 formula of black ganoderma mycelium pellet culture solution: 8-10g of peptone, 0.01-0.02g of plant charcoal melanin and 38-40g of glucose, and adding water to 1L;
the pH value of each culture solution formula is regulated to 6.8-7.2 by 0.1-0.2mol/L sodium hydroxide solution;
(2) treating the ganoderma lucidum seed liquid: grinding Ganoderma seed solution into slurry under aseptic condition with homogenizer, transferring into glass injector and sleeving with replaceable membrane chromatographic filter, and filtering with filter paper instead of membrane to obtain mycelium suspension;
(3) culturing: and (3) taking the culture solution obtained in the step (1), adding 2-4mL of hypha suspension into every 1L of culture solution, transferring the hypha suspension obtained in the step (2), transferring the hypha suspension into the culture solution, carrying out vortex oscillation for 20-30 min, then, culturing for 1-2 days at 26-28 ℃ and 160-200 r/min, standing for 2-4 h at the same temperature, and then, culturing for 2-3 days at 26-28 ℃ and 60-100 r/min to obtain the seven-color ganoderma mycelium pellet product.
2. The use of claim 1, wherein the mycelium pellets are cultured directionally to form a hedgehog ball, as follows: preparing a chitosan solution with the concentration of 0.2-0.3g/mL by using a glacial acetic acid aqueous solution with the volume concentration of 0.5-1.5% as a solvent, and adding the chitosan solution into the culture solution in the step (1) according to the amount of adding 1-2mL of chitosan solution into every 1000mL of culture solution.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103125274A (en) * | 2013-03-15 | 2013-06-05 | 福建农林大学 | Grafting method between different types of ganoderma lucidum and potted landscape process |
| CN103460999A (en) * | 2013-09-13 | 2013-12-25 | 湖南省致远农业科技发展有限公司 | Method for cultivating ornamental lucid ganoderma by using waste fungus for producing pleurotus eryngii in industrialized manner |
| CN107384805A (en) * | 2017-08-25 | 2017-11-24 | 山西农业大学 | A kind of production method of edible mushroom rejuvenation type bacterial strain |
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| US6558943B1 (en) * | 2000-09-05 | 2003-05-06 | Sun Ten Pharmaceutical Co., Ltd. | Method for propagating fungi using solid state fermentation |
| CN102071146B (en) * | 2010-10-27 | 2013-07-31 | 广州大学 | Culture method and applications of Ganoderma weberianum mycelial pellets |
| CN102550285A (en) * | 2012-01-09 | 2012-07-11 | 元拓(福州)生物技术有限公司 | Production method for mycelium pellets of edible and medicinal fungi and products of mycelium pellets of edible and medicinal fungi |
| CN106244462A (en) * | 2016-07-29 | 2016-12-21 | 湖南农业大学 | A kind of ganoderma lucidum mycelium liquid fermentation culture method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103125274A (en) * | 2013-03-15 | 2013-06-05 | 福建农林大学 | Grafting method between different types of ganoderma lucidum and potted landscape process |
| CN103460999A (en) * | 2013-09-13 | 2013-12-25 | 湖南省致远农业科技发展有限公司 | Method for cultivating ornamental lucid ganoderma by using waste fungus for producing pleurotus eryngii in industrialized manner |
| CN107384805A (en) * | 2017-08-25 | 2017-11-24 | 山西农业大学 | A kind of production method of edible mushroom rejuvenation type bacterial strain |
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| Comparison crushing methods of Ganoderma lucidum mycelium;Ke-xin Qin等;《Science and Technology of Food Industry》;20161231(第9期);第146-149页 * |
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