CN104045461B - A kind of Cordyccps-militaris-(L.)-link. Sporophore substratum adding Flower of Arabian Jasmine slag - Google Patents
A kind of Cordyccps-militaris-(L.)-link. Sporophore substratum adding Flower of Arabian Jasmine slag Download PDFInfo
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Abstract
The invention discloses a kind of Cordyccps-militaris-(L.)-link. Sporophore substratum adding Flower of Arabian Jasmine slag, be made up of the raw material of following weight part: nutritive medium 40 ~ 50 parts, major ingredient 35 ~ 45 parts, dried silkworm chrysalis meal 4 ~ 11 parts, Flower of Arabian Jasmine slag 8 ~ 15 parts.Use the Cordyccps-militaris-(L.)-link. Sporophore substratum of interpolation Flower of Arabian Jasmine slag of the present invention, effectively can suppress varied bacteria growing in Cordyccps-militaris-(L.)-link. Sporophore process of growth, improve the seed output and quality of Cordyceps militaris (L.) Link., the anti-oxidation efficacy of Cordyceps militaris (L.) Link. can be strengthened simultaneously.In addition, the Flower of Arabian Jasmine slag added as waste disposal in Cordyccps-militaris-(L.)-link. Sporophore substratum of the present invention is cheap, realizes resource and makes full use of, and reduces substratum more than 18% cost.
Description
Technical field
The present invention relates to culture medium of edible fungus field, particularly a kind of Cordyccps-militaris-(L.)-link. Sporophore substratum adding Flower of Arabian Jasmine slag.
Background technology
Cordyceps militaris (L.) Link., also known as Cordyceps militaris or Cordyceps militaris (L.) Link., belongs to Mycophyta, Ascomycotina, Hypocreales, Clavicipitaceae, Cordyceps, parasitizes on the pupal cells such as Noctuidae, is distributed in all over the world, is a kind of not only edible but also pharmaceutically useful fungi of generally acknowledging both at home and abroad.Cordyceps militaris (L.) Link. and Cordyceps sinensis belong to xenogenesis together, are the widest two kinds of Cordyceps with pharmaceutical use of China's distribution.Research shows that Cordyceps militaris (L.) Link. and Cordyceps sinensis have same alike result, and similar pharmacological function and detailed clinical effectiveness, alternative Cordyceps sinensis is used as medicine.Containing compositions such as cordycepin, Cordyceps polysaccharide, cordycepic acid, adenosines in Cordyceps militaris (L.) Link.; have and strengthen immune, anti-oxidant, antiviral, antibacterial, obvious Tumor suppression growth, prophylactic treatment cerebral thrombosis, Intracerebral hemorrhage, renal failure; diuresis, suppression Platelet congregation prevent thrombosis; elimination face spot; anti-ageing wrinkle resistant, cardioprotection, liver, the several functions such as anti-convulsion.
Wild Chinese caterpillar fungus resource-constrained, therefore people start then study the artificial culture with Chinese caterpillar fungus.Cordyceps militaris (L.) Link. is relatively low for the requirement of growing environment; artificial culture is easier to by contrast; main with liquid fermenting formation mycelium at present, obtain sporophore by artificial extensive solid culture, carry out the production of mass-producing Cordyceps militaris (L.) Link. additionally by live body cultivation and become a reality.In culturing process, if sterilization is bad or sterilization is not tight, easily there is the pollutions such as bacterium, yeast and mould in Cordyceps militaris (L.) Link..If there is bacterium, yeast and mould contamination, will be further purified master clock, serious will be eliminated.Bacterium, yeast and mould are to the pollution of Cordyceps militaris (L.) Link. as can be seen here, will cause and cultivate financial resources, material resources loss, and increase unnecessary trouble.Find a kind of new demand having the substratum of bacteria resistance function to become Chinese caterpillar fungus culture medium field.
Flower of Arabian Jasmine is a kind of natural fine perfumery plant.Flower of Arabian Jasmine slag is the dry flower after jasmine fresh flower scenting tea, or extracts the discarded bud after essential oil, usually used as waste disposal.In scented tea baking process, tealeaves only absorbs a part for the aromatoising substance in Flower of Arabian Jasmine.Still there is a large amount of valuable aromatoising substance and trace element in flower slag, still have very large utility value.The Lin Jinfang nutritive ingredient that have studied Flower of Arabian Jasmine slag comparatively early, thinks the similar summary of the nutritive value of Flower of Arabian Jasmine slag or be better than wheat bran, rice bran class.The research discovery 10% Flower of Arabian Jasmine slags such as leaf shines can replace the wheat bran of 2% soya-bean cake and 85%, illustrate that Flower of Arabian Jasmine slag still has higher nutritive value.King sends out the left main inclusion composition that comparatively system have studied in Flower of Arabian Jasmine slag, and result shows moisture content 5.97% in Flower of Arabian Jasmine slag, crude protein 20.8%, crude fat 2.4%, robust fibre 12.4%, water-soluble carbohydrate 8.04%, total free aminoacids 3.41%, vitamins C 0.21%.Total free aminoacids detects in 15, wherein containing in essential amino acid 6; Detect protein hydrolytic amino acid 16 kinds, wherein in essential amino acid 7.Except containing above-mentioned natural product composition during king sends out research Flower of Arabian Jasmine slag left, also containing abundant Flavonoid substances, reach more than 3%.Flavonoid compound has sterilization, anti-oxidant, anti-ageing, cancer-resisting, the multiple physiologically active such as hypoglycemic.Flower of Arabian Jasmine has larger cultivated area in China, aboundresources, and scenting tea and extraction essential oil, medicinal extract produce a large amount of Flower of Arabian Jasmine slags, as waste disposal, and waste resource.Flower of Arabian Jasmine slag is applied in Cordyccps-militaris-(L.)-link. Sporophore substratum there is wide market outlook and economic worth.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of suppression varied bacteria growing, strengthens the Cordyccps-militaris-(L.)-link. Sporophore substratum of the interpolation Flower of Arabian Jasmine slag of Cordyceps militaris (L.) Link. antioxygenation.
For achieving the above object, the present invention adopts following steps:
Add a Cordyccps-militaris-(L.)-link. Sporophore substratum for Flower of Arabian Jasmine slag, be made up of the raw material of following weight part: nutritive medium 40 ~ 50 parts, major ingredient 35 ~ 45 parts, dried silkworm chrysalis meal 4 ~ 11 parts, Flower of Arabian Jasmine slag 8 ~ 15 parts.
The Cordyccps-militaris-(L.)-link. Sporophore substratum of interpolation Flower of Arabian Jasmine slag of the present invention, is preferably made up of the raw material of following weight part: nutritive medium 44 ~ 47 parts, major ingredient 38 ~ 42 parts, dried silkworm chrysalis meal 7 ~ 9 parts, Flower of Arabian Jasmine slag 10 ~ 13 parts.
Major ingredient of the present invention is one or more in rice, wheat, corn, soya bean, when multiple mixing, can be arbitrary proportion.Suitably add carbon source in the medium, as rice, wheat, corn, soya bean, be conducive to Cordyceps militaris (L.) Link. synthetic carbohydrate and amino acid.
Nutritive medium of the present invention is made up of the raw material of following weight part: sugar 0.7 ~ 1.5 part, yeast extract paste 0.7 ~ 1.2 part, potassium primary phosphate 0.01 ~ 0.03 part, 0.01 ~ 0.03 part, magnesium sulfate, ammonium citrate 0.01 ~ 0.03 part, vitaminB10 .001 ~ 0.003 part, 94 ~ 98 parts, water.Generally meet the demand of Cordyceps militaris (L.) Link. to mineral element by adding inorganic salts, preferably sulfuric acid magnesium of the present invention, potassium primary phosphate, wherein potassium primary phosphate also has the pH value effect regulating substratum, substratum is adjusted to slant acidity, meets Cordyceps militaris (L.) Link. slant acidity demand.Because Cordyceps militaris (L.) Link. can not synthesize essential vitamin, suitably add vitamins B
1be conducive to growing of Cordyceps militaris (L.) Link..Suitably add nitrogen element inorganic salt in the medium, as ammonium citrate, promote the inorganic nitrogen such as organonitrogen and ammonium salt such as protein, nucleic acid of Cordyceps militaris (L.) Link. self synthesis.
Sugar of the present invention is glucose or sucrose.Dextrose plus saccharoses etc. are small molecules carbohydrate, also as Cordyceps militaris (L.) Link. carbon source, promote Cordyceps militaris (L.) Link. synthetic carbohydrate and amino acid whose effect best.
For suppressing varied bacteria growing, strengthen Cordyceps militaris (L.) Link. antioxygenation, the present invention adds Flower of Arabian Jasmine slag in the medium, and Flower of Arabian Jasmine slag of the present invention is the dry flower after jasmine fresh flower scenting tea, or extracts the dry flower after essential oil, medicinal extract.
The present invention adds the preparation of the Cordyccps-militaris-(L.)-link. Sporophore substratum of Flower of Arabian Jasmine slag, and step is as follows:
(1) raw material pre-treatment: by Flower of Arabian Jasmine slag after being ground into powder, crosses 20 ~ 50 mesh sieves, then major ingredient is ground into fine powder, crosses 50 ~ 100 mesh sieves; Take sugar, yeast extract paste, dipotassium hydrogen phosphate, magnesium sulfate, ammonium citrate, add water, be heated to 90 DEG C ~ 100 DEG C while stirring, continue stirring until raw material and all dissolve, be cooled to envrionment temperature, add VITMAIN B1, obtain nutritive medium;
(2) raw material mixing: major ingredient fine powder, Flower of Arabian Jasmine ground-slag end, dried silkworm chrysalis meal are mixed, mixture is added in nutritive medium while stirring, stirs, obtain mixed solution;
(3) bottle: divided by mixed solution and be filled in culture vessel, Intake Quantity is 2/3 of culture vessel actual capacity;
(4) sterilization: mixed solution container sterilization 30 ~ 45min under 119 DEG C ~ 124 DEG C high temperature will be housed, be cooled to 25 DEG C ~ 27 DEG C, obtains the Cordyccps-militaris-(L.)-link. Sporophore substratum adding Flower of Arabian Jasmine slag.
Compared with prior art, beneficial effect of the present invention is:
1, abundant flavones and flavones ingredient is contained in Flower of Arabian Jasmine slag, flavones in Flower of Arabian Jasmine slag and flavones ingredient are mould to proteus vulgaris, staphylococcus, subtilis, intestinal bacteria, wood, head mold has certain restraining effect, are added in Cordyccps-militaris-(L.)-link. Sporophore substratum by Flower of Arabian Jasmine slag and reach antibacterial object.
2, the composition Wheat Protein in Flower of Arabian Jasmine slag, the present invention, by adding Flower of Arabian Jasmine slag in Cordyccps-militaris-(L.)-link. Sporophore substratum, can strengthen Cordyceps militaris (L.) Link. anti-oxidation efficacy.
3, the Heng County, the southeast in Guangxi abounds with Flower of Arabian Jasmine, seed output and quality ranks first in the whole country, scenting tea and extraction essential oil, medicinal extract produce a large amount of Flower of Arabian Jasmine slags, as waste disposal, and waste resource, the present invention adds Flower of Arabian Jasmine slag in Cordyccps-militaris-(L.)-link. Sporophore substratum, Flower of Arabian Jasmine slag can be made full use of, turn waste into wealth, realize the recycle of resource, promote the value of Flower of Arabian Jasmine slag, reduce Cordyccps-militaris-(L.)-link. Sporophore substratum more than 18% cost.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but the present invention is not limited to these embodiments.
Embodiment 1
Add a Cordyccps-militaris-(L.)-link. Sporophore substratum for Flower of Arabian Jasmine slag, be made up of the raw material of following weight part: nutritive medium 40 parts, 35 parts, rice, dried silkworm chrysalis meal 4 parts, Flower of Arabian Jasmine slag 8 parts.
Nutritive medium, is made up of the raw material of following weight part: glucose 0.7 part, yeast extract paste 0.7 part, potassium primary phosphate 0.01 part, 0.01 part, magnesium sulfate, ammonium citrate 0.01 part, vitaminB10 .001 part, 94 parts, water.
The preparation of nutritive medium: take glucose, yeast extract paste, dipotassium hydrogen phosphate, magnesium sulfate, ammonium citrate, add water, is heated to 90 DEG C while stirring, continues stirring until raw material and all dissolves, be cooled to envrionment temperature, add VITMAIN B1, obtain nutritive medium.
Add the preparation of the Cordyccps-militaris-(L.)-link. Sporophore substratum of Flower of Arabian Jasmine slag, step is as follows:
(1) raw material pre-treatment: by Flower of Arabian Jasmine slag after being ground into powder, crosses 20 mesh sieves, then rice meal is broken into fine powder, crosses 50 mesh sieves;
(2) raw material mixing: rice fine powder, Flower of Arabian Jasmine ground-slag end, dried silkworm chrysalis meal are mixed, mixture is added in nutritive medium while stirring, stirs, obtain mixed solution;
(3) bottle: divided by mixed solution and be filled in culture vessel, Intake Quantity is 2/3 of culture vessel actual capacity;
(4) sterilization: mixed solution container sterilization 45min under 119 DEG C of high temperature will be housed, be cooled to 25 DEG C, obtains the Cordyccps-militaris-(L.)-link. Sporophore substratum adding Flower of Arabian Jasmine slag.
Embodiment 2
Add a Cordyccps-militaris-(L.)-link. Sporophore substratum for Flower of Arabian Jasmine slag, be made up of the raw material of following weight part: nutritive medium 44 parts, 25 parts, rice, soya bean 13 parts, dried silkworm chrysalis meal 7 parts, Flower of Arabian Jasmine slag 10 parts.
Nutritive medium, is made up of the raw material of following weight part: sucrose 0.9 part, yeast extract paste 0.9 part, potassium primary phosphate 0.02 part, 0.02 part, magnesium sulfate, ammonium citrate 0.02 part, vitaminB10 .002 part, 96 parts, water.
The preparation of nutritive medium: take sucrose, yeast extract paste, dipotassium hydrogen phosphate, magnesium sulfate, ammonium citrate, add water, is heated to 95 DEG C while stirring, continues stirring until raw material and all dissolves, be cooled to envrionment temperature, add VITMAIN B1, obtain nutritive medium.
Add the preparation of the Cordyccps-militaris-(L.)-link. Sporophore substratum of Flower of Arabian Jasmine slag, step is as follows:
(1) raw material pre-treatment: by Flower of Arabian Jasmine slag after being ground into powder, crosses 30 mesh sieves, then rice, analysis for soybean powder is broken into fine powder, crosses 60 mesh sieves;
(2) raw material mixing: rice fine powder, soya bean fine powder, Flower of Arabian Jasmine ground-slag end, dried silkworm chrysalis meal are mixed, mixture is added in nutritive medium while stirring, stirs, obtain mixed solution;
(3) bottle: divided by mixed solution and be filled in culture vessel, Intake Quantity is 2/3 of culture vessel actual capacity;
(4) sterilization: mixed solution container sterilization 40min under 121 DEG C of high temperature will be housed, be cooled to 26 DEG C, obtains the Cordyccps-militaris-(L.)-link. Sporophore substratum adding Flower of Arabian Jasmine slag.
Embodiment 3
Add a Cordyccps-militaris-(L.)-link. Sporophore substratum for Flower of Arabian Jasmine slag, be made up of the raw material of following weight part: nutritive medium 47 parts, corn 12 parts, wheat 20 parts, soya bean 10 parts, dried silkworm chrysalis meal 9 parts, Flower of Arabian Jasmine slag 13 parts.
Nutritive medium, is made up of the raw material of following weight part: sucrose 1.2 parts, yeast extract paste 1.0 parts, potassium primary phosphate 0.03 part, 0.03 part, magnesium sulfate, ammonium citrate 0.03 part, vitaminB10 .003 part, 97 parts, water.
The preparation of nutritive medium: take sucrose, yeast extract paste, dipotassium hydrogen phosphate, magnesium sulfate, ammonium citrate, add water, is heated to 95 DEG C while stirring, continues stirring until raw material and all dissolves, be cooled to envrionment temperature, add VITMAIN B1, obtain nutritive medium.
Add the preparation of the Cordyccps-militaris-(L.)-link. Sporophore substratum of Flower of Arabian Jasmine slag, step is as follows:
(1) raw material pre-treatment: by Flower of Arabian Jasmine slag after being ground into powder, crosses 40 mesh sieves, then corn, wheat, analysis for soybean powder is broken into fine powder, crosses 80 mesh sieves;
(2) raw material mixing: corn fine powder, wheat fine powder, soya bean fine powder, Flower of Arabian Jasmine ground-slag end, dried silkworm chrysalis meal are mixed, mixture is added in nutritive medium while stirring, stirs, obtain mixed solution;
(3) bottle: divided by mixed solution and be filled in culture vessel, Intake Quantity is 2/3 of culture vessel actual capacity;
(4) sterilization: mixed solution container sterilization 35min under 122 DEG C of high temperature will be housed, be cooled to 27 DEG C, obtains the Cordyccps-militaris-(L.)-link. Sporophore substratum adding Flower of Arabian Jasmine slag.
Embodiment 4
Add a Cordyccps-militaris-(L.)-link. Sporophore substratum for Flower of Arabian Jasmine slag, be made up of the raw material of following weight part: nutritive medium 50 parts, 25 parts, rice, corn 20 parts, dried silkworm chrysalis meal 11 parts, Flower of Arabian Jasmine slag 15 parts.
Nutritive medium is made up of the raw material of following weight part: glucose 1.5 parts, yeast extract paste 1.2 parts, potassium primary phosphate 0.02 part, 0.02 part, magnesium sulfate, ammonium citrate 0.02 part, vitaminB10 .002 part, 98 parts, water.
The preparation of nutritive medium: take glucose, yeast extract paste, dipotassium hydrogen phosphate, magnesium sulfate, ammonium citrate, add water, is heated to 100 DEG C while stirring, continues stirring until raw material and all dissolves, be cooled to envrionment temperature, add VITMAIN B1, obtain nutritive medium.
Add the preparation of the Cordyccps-militaris-(L.)-link. Sporophore substratum of Flower of Arabian Jasmine slag, step is as follows:
(1) raw material pre-treatment: by Flower of Arabian Jasmine slag after being ground into powder, crosses 50 mesh sieves, then rice, Semen Maydis powder is broken into fine powder, crosses 100 mesh sieves;
(2) raw material mixing: rice fine powder, corn fine powder, Flower of Arabian Jasmine ground-slag end, dried silkworm chrysalis meal are mixed, mixture is added in nutritive medium while stirring, stirs, obtain mixed solution;
(3) bottle: divided by mixed solution and be filled in culture vessel, Intake Quantity is 2/3 of culture vessel actual capacity;
(4) sterilization: mixed solution container sterilization 30min under 124 DEG C of high temperature will be housed, be cooled to 25 DEG C, obtains the Cordyccps-militaris-(L.)-link. Sporophore substratum adding Flower of Arabian Jasmine slag.
Comparative example
A kind of Cordyccps-militaris-(L.)-link. Sporophore substratum, is made up of the raw material of following weight part: nutritive medium 44 parts, 30 parts, rice, soya bean 15 parts, dried silkworm chrysalis meal 9 parts.
Nutritive medium, is made up of the raw material of following weight part: sucrose 0.9 part, yeast extract paste 0.9 part, potassium primary phosphate 0.02 part, 0.02 part, magnesium sulfate, ammonium citrate 0.02 part, vitaminB10 .002 part, 96 parts, water.
The preparation of nutritive medium: take sucrose, yeast extract paste, dipotassium hydrogen phosphate, magnesium sulfate, ammonium citrate, add water, is heated to 95 DEG C while stirring, continues stirring until raw material and all dissolves, be cooled to envrionment temperature, add VITMAIN B1, obtain nutritive medium.
The preparation of Cordyccps-militaris-(L.)-link. Sporophore substratum, step is as follows:
(1) raw material pre-treatment: rice, analysis for soybean powder are broken into fine powder, crosses 60 mesh sieves;
(2) raw material mixing: rice fine powder, soya bean fine powder, dried silkworm chrysalis meal are mixed, mixture is added in nutritive medium while stirring, stirs, obtain mixed solution;
(3) bottle: divided by mixed solution and be filled in culture vessel, Intake Quantity is 2/3 of culture vessel actual capacity;
(4) sterilization: mixed solution container sterilization 40min under 121 DEG C of high temperature will be housed, be cooled to 26 DEG C, obtain Cordyccps-militaris-(L.)-link. Sporophore substratum.
Test example 1: the present invention adds the Cordyccps-militaris-(L.)-link. Sporophore substratum of Flower of Arabian Jasmine slag to the restraining effect of miscellaneous bacteria
1, medium liquid preparation: the embodiment of the present invention 1, embodiment 2, embodiment 3, embodiment 4, comparative example are extracted three times, united extraction liquid, be concentrated into certain volume, add the alcohol settling of 3 times 95%, get supernatant concentration, drying, obtain embodiment 1, embodiment 2, embodiment 3, embodiment 4, comparative example substratum crude extract are for subsequent use.
2, cultivate and qualification for examination bacterial classification and purifying thereof:
Bacillus proteus, staphylococcus, subtilis, intestinal bacteria, provided by Guangxi University's Animal Science And Technology.
Bacterial classification adopts continuous method of scoring to carry out purifying, and bacterium colony after cultivation adopts gram staining method to differentiate, the strain inclined plane after discriminating is cultivated, for subsequent use.
Gram staining method: bacterium first through basic dyestuff violet staining, and after the mordant dyeing of iodine liquid, is decoloured with alcohol, the bacterium purple had under certain condition is not divested, and what have is divested, and therefore bacterium can be divided into two large classes, the former is called gram-positive microorganism, and the latter is Gram-negative bacteria.For observing conveniently, redye by a kind of orchil red, dilution azaleine as luxuriant in alkalescence etc. again after decolouring.Positive bacteria is still with purple, and negative bacterium is then by red-dyed.Have the bacillus of brood cell and most coccuses, and all actinomycetes and fungi are all gram positive reaction; Vibrios, spirochete and most of pathogenic bactacin all present negative reaction.
3, the preparation of bacteria culture medium:
Adopt beef-protein medium, in order to increase the speed of growth of bacterium, add 10g glucose in every 1kg water to improve substratum, 0.1Mpa sterilizing 20min, makes flat board.
4, for the preparation of examination bacterium liquid:
The purified bacterium colony with identifying of picking, is inoculated in broth medium, and 36 DEG C of constant-temperature shaking culture are to muddy.Then draw a certain amount of bacterium liquid to be inoculated in several Boiling tubes, repeat above-mentioned CMC model.Bacterium liquid 10 times dilution, gets 0.1ml respectively and is applied to flat board, and 36 DEG C of constant temperature culture, bacterium colony forms rear counting, makes 5 × 10
8cFU/ml bacterium liquid.
4, punch tool method measures the present invention and adds the Cordyccps-militaris-(L.)-link. Sporophore substratum of Flower of Arabian Jasmine slag to the restraining effect of miscellaneous bacteria:
Punch on flat board with the punch tool of R=0.6cm, coating is own through ready bacterium liquid, and in hole, then add the solution that concentration is the different culture media crude extract for subsequent use of 100g/L, 37 DEG C of constant temperature culture were observed after 2 ~ 3 days, surveyed the size of its inhibition zone.
6, test-results:
Table 1 adds the size statistical unit of different Cordyccps-militaris-(L.)-link. Sporophore culture medium solution inhibition zone radius: cm
As can be seen from Table 1, the Cordyccps-militaris-(L.)-link. Sporophore substratum of the interpolation Flower of Arabian Jasmine slag of the embodiment of the present invention 1, embodiment 2, embodiment 3, embodiment 4 has stronger restraining effect to Bacillus proteus, staphylococcus, subtilis, intestinal bacteria, and the Cordyccps-militaris-(L.)-link. Sporophore substratum not adding Flower of Arabian Jasmine slag of comparative example is extremely faint to miscellaneous bacteria restraining effect.
Test example 2: the Cordyceps militaris (L.) Link. antioxygenation that the present invention cultivates
1, material: the Cordyceps militaris (L.) Link. that the embodiment of the present invention 3, embodiment 4, comparative example are cultivated.
2, test method:
(1) Cordyccps-militaris-(L.)-link. Sporophore runic thing prepares extracting method: pulverized respectively by the Cordyceps militaris (L.) Link. that the embodiment of the present invention 3, embodiment 4, comparative example are cultivated, by solid-liquid ratio 1:25 extracting in water, extract three times, united extraction liquid, be concentrated into certain volume, add the alcohol settling of 3 times 95%, get supernatant concentration, drying, obtain Cordyceps militaris (L.) Link. crude extract for subsequent use.
(2) mensuration of reducing power: sample is by the Tripotassium iron hexacyanide (K
3fe (CN)
6) be reduced into yellow prussiate of potash (K
4fe (CN)
6), yellow prussiate of potash again with Fe
3+effect, generate ferriferro cyanide (Prussian blue), to detect the size that Prussian blue absorbancy represents reducing power at 700nm wavelength place, absorbancy is higher, and the reducing power of sample is stronger, and oxidation-resistance is stronger.At 700nm wavelength place, measure the light absorption value of the Cordyceps militaris (L.) Link. crude extract that concentration is the embodiment of the present invention 3 of 5mg/ml, embodiment 4, comparative example are cultivated respectively.
(3) superoxide anion experiment is removed: at generation ultra-oxygen anion free radical (O
2-) reaction system in, add the embodiment of the present invention 3 that concentration is 5mg/ml respectively, Cordyceps militaris (L.) Link. crude extract that embodiment 4, comparative example are cultivated, measure their clearance rates.
(4) experiment of hydroxyl radical free radical is removed: FeSO
4with H
2o
2reaction produces OH, and in system, add Whitfield's ointment, can catch hydroxyl radical free radical and produce coloring matter, this material has maximum absorption at 510nm place, and when there is OH free-radical scavengers in reaction system, this oxidising process is suppressed, and absorbance then reduces.At FeSO
4with H
2o
2in reaction system, add the embodiment of the present invention 3 that concentration is 30mg/ml respectively, Cordyceps militaris (L.) Link. crude extract that embodiment 4, comparative example are cultivated, measure clearance rate.
(5) NO under simulated gastric fluid pH condition
2-cleaning reaction effect: cleaning nitrite is one of approach effectively preventing N-nitroso compound carcinogenic.Add in simulated system and add the embodiment of the present invention 3 that concentration is 14mg/ml respectively, Cordyceps militaris (L.) Link. crude extract that embodiment 4, comparative example are cultivated, measure clearance rate.
(6) DPPH method measures Cordyceps militaris (L.) Link. anti-oxidant activity: DPPH is a kind of stable free radical in organic solvent, is widely used in anti-oxidant appraisement system.In system, add the embodiment of the present invention 3 that concentration is 30mg/ml respectively, Cordyceps militaris (L.) Link. crude extract that embodiment 4, comparative example are cultivated, measure clearance rate.
3, test-results:
The reducing power of the Cordyceps militaris (L.) Link. crude extract that table 1 different culture media is cultivated
| Embodiment 3 | Embodiment 4 | Comparative example | |
| Light absorption value | 1.337 | 1.323 | 0.825 |
Table 1 result shows, the Cordyceps militaris (L.) Link. crude extract that embodiment 3, embodiment 4, comparative example are cultivated has oxidation-resistance, and embodiment 3, embodiment 4 oxidation-resistance are better than comparative example.
The Cordyceps militaris (L.) Link. crude extract that table 2 different culture media is cultivated removes the ability of superoxide anion
| Embodiment 3 | Embodiment 4 | Comparative example | |
| Clearance rate | 34％ | 29％ | 16％ |
Table 2 shows, the Cordyceps militaris (L.) Link. crude extract that embodiment 3, embodiment 4, comparative example are cultivated all has the ability removing superoxide anion, and embodiment 3, embodiment 4 Scavenging activity are better than comparative example.
The Cordyceps militaris (L.) Link. crude extract that table 3 different culture media is cultivated removes the ability of hydroxyl radical free radical
| Embodiment 3 | Embodiment 4 | Comparative example | |
| Clearance rate | 77％ | 73％ | 52％ |
Table 3 shows, the Cordyceps militaris (L.) Link. crude extract that embodiment 3, embodiment 4, comparative example are cultivated all has the ability removing hydroxyl radical free radical, and embodiment 3, embodiment 4 Scavenging activity are better than comparative example.
The Cordyceps militaris (L.) Link. crude extract that table 4 different culture media is cultivated is to NO
2-scavenging(action)
| Embodiment 3 | Embodiment 4 | Comparative example | |
| Clearance rate | 55％ | 50％ | 35％ |
Table 4 shows, the Cordyceps militaris (L.) Link. crude extract that embodiment 3, embodiment 4, comparative example are cultivated is to NO
2-have scavenging(action), embodiment 3, embodiment 4 Scavenging activity are better than comparative example.
The Cordyceps militaris (L.) Link. crude extract that table 5 different culture media is cultivated is to DPPH elimination effect
| Embodiment 3 | Embodiment 4 | Comparative example | |
| Clearance rate | 66％ | 63％ | 26％ |
Table 5 shows, the Cordyceps militaris (L.) Link. crude extract that embodiment 3, embodiment 4, comparative example are cultivated has elimination effect to DPPH, and embodiment 3, embodiment 4 elimination effect are better than comparative example.
Comprehensive above-mentioned oxidizing reaction system test-results, the Cordyceps militaris (L.) Link. that known embodiment 3, embodiment 4, comparative example are cultivated has oxidation-resistance, and embodiment 3, embodiment 4 are better than comparative example, illustrate that the Cordyccps-militaris-(L.)-link. Sporophore substratum adding Flower of Arabian Jasmine slag can strengthen the anti-oxidation efficacy of Cordyceps militaris (L.) Link..
Claims (6)
1. add a Cordyccps-militaris-(L.)-link. Sporophore substratum for Flower of Arabian Jasmine slag, it is characterized in that, be made up of the raw material of following weight part: nutritive medium 40 ~ 50 parts, major ingredient 35 ~ 45 parts, dried silkworm chrysalis meal 4 ~ 11 parts, Flower of Arabian Jasmine slag 8 ~ 15 parts.
2. the Cordyccps-militaris-(L.)-link. Sporophore substratum of interpolation Flower of Arabian Jasmine slag according to claim 1, is characterized in that, be made up of the raw material of following weight part: nutritive medium 44 ~ 47 parts, major ingredient 38 ~ 42 parts, dried silkworm chrysalis meal 7 ~ 9 parts, Flower of Arabian Jasmine slag 10 ~ 13 parts.
3. the Cordyccps-militaris-(L.)-link. Sporophore substratum of interpolation Flower of Arabian Jasmine slag according to claim 1 and 2, is characterized in that: described major ingredient is one or more in rice, wheat, corn, soya bean.
4. the Cordyccps-militaris-(L.)-link. Sporophore substratum of interpolation Flower of Arabian Jasmine slag according to claim 1 and 2, it is characterized in that, described nutritive medium is made up of the raw material of following weight part: sugar 0.7 ~ 1.5 part, yeast extract paste 0.7 ~ 1.2 part, potassium primary phosphate 0.01 ~ 0.03 part, 0.01 ~ 0.03 part, magnesium sulfate, ammonium citrate 0.01 ~ 0.03 part, vitaminB10 .001 ~ 0.003 part, 94 ~ 98 parts, water.
5. the Cordyccps-militaris-(L.)-link. Sporophore substratum of interpolation Flower of Arabian Jasmine slag according to claim 4, is characterized in that: described sugar is glucose or sucrose.
6. the Cordyccps-militaris-(L.)-link. Sporophore substratum of interpolation Flower of Arabian Jasmine slag according to claim 1 and 2, is characterized in that: described Flower of Arabian Jasmine slag is the dry flower after jasmine fresh flower scenting tea, or extracts the dry flower after essential oil, medicinal extract.
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