CN104232441A - Health caring and preserving cordyceps wine and preparation method thereof - Google Patents
Health caring and preserving cordyceps wine and preparation method thereof Download PDFInfo
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Abstract
The invention provides health caring and preserving cordyceps wine containing more metabolic substance active ingredients. The invention further provides a preparation method of the health caring and preserving cordyceps wine. According to the preparation method, the biotransformation rate is greatly improved by optimizing cultivation conditions, the content of the metabolic substance active ingredients is greatly increased, the inherent value of cordyceps is fully played, the quick rancidification of fermentation liquid in the fermentation cultivation process can be prevented, and more specific-direction metabolic substances can be more effectively obtained.
Description
Technical field
The present invention relates to a kind of wine with nourishing function, particularly a kind of health care health cordyceps militaris liquor and preparation method thereof.
Background technology
At present, with Cordyceps sinensis etc., for basic raw material, to prepare similar healthcare products kind a lot, but generally all select the sporophore of natural cs and mushroom, glossy ganoderma, mix after pulverizing; Or carry out solid fermentation, dry together with substratum, pulverize, choose, granulation, packaging; Or carry out liquid submerged fermentation, thalline is filtered out, reconcentration fermented liquid.The first limit due to natural resource, and cause and yield poorly, cost is high, is difficult to apply, meets the need of market.The more original backwardness of the second solid fermentation, biological transformation ratio is low, though can reduce certain cost, because of can not scale production, easily pollutes and causes loss, being also eliminated gradually.The third deep fermentation, owing to being filtered out by thalline, affects some active ingredient that natural matter has.
Existing application number is the Cordyceps mycelium fermenation raw liquid of the patent employing of 201210257839.4 is employing specifically fermentation technology, obtain the whole beer that fermentation thalli stoste is lumped together, carry out cytoclasis again, concentrate and be equipped with several Chinese medicine and modulate, both natural cordyceps effective ingredient can be kept, play special medical health-care effect, but its metabolic substd activeconstituents is limited, effectively can not obtains more single-minded direction metabolic substd; And the natural pH value of substratum is 6.2, culture cycle is 48 hours, and terminal pH value is 3.6-3.8, and the top condition of uncontrollable whole fermentation, also largely have impact on the quality of product.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of health care health cordyceps militaris liquor containing more metabolic substd activeconstituents, present invention also offers the preparation method of described health care health cordyceps militaris liquor, this preparation method makes biological transformation ratio greatly improve by optimum culture condition, larger improves metabolic substd active component content, fully play the value that Chinese caterpillar fungus originally has, and can prevent fermentation culture process fermented liquid from becoming sour rapidly, more effectively obtain more single-minded direction metabolic substd.
The invention provides a kind of preparation method of health care health cordyceps militaris liquor, comprise the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 5 ~ 7 days at 23 ~ 25 DEG C;
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium and weight as follows: 180 ~ 220 grams, potato, glucose 18 ~ 22 grams, peptone 2 ~ 4 grams, yeast extract paste 0.5 ~ 1.5 gram, 0.4 ~ 0.6 gram, magnesium sulfate, potassium primary phosphate 0.5 ~ 1.5 gram, natural pH value, incubation time 36 ~ 42 hours at 23 ~ 25 DEG C, wherein said liquid nutrient medium is in 1000ml;
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating;
Wherein corresponding one-level, secondary, three grades of submerged fermentation culture mediums are all identical, its composition and weight are: millet 30-35g, glucose 20-25g, white sugar 1-2g, potassium dihydrogen sulfate 0.3-1.2g, magnesium sulfate 0.1-0.15g, peptone 3-5g, yeast extract 1-2g, amino acid 0.1-0.2g, vitamin V B10.005-0.015g, adds water to 1000ml;
Its process is as follows: with 8-10% inoculum size access liquid spawn, at 22-23 DEG C, under the condition that ventilating ratio 1:0.5 ~ 0.7 and the interior pressure of cultivation are 0.03-0.05MPa, continuously ferment 36-38 hour, now PH is 4.5 ~ 5.0, mycelium natural subsidence 100%, in aforementioned process, the condition of one-level, secondary, three grades of submerged fermentations is all identical;
D. active bacteria somatocyte broken wall
By live body bacterium liquid through the grinding of 3T/H colloidal mill, then use 3T/H, processing pressure is the high pressure homogenizer fracturing cell walls of 40MPa, form suspendible glue, at 32 ~ 42 DEG C, be incubated placement 1 ~ 2 hour, intracellular matter is fully oozed out, obtain Cordyceps mycelium fermenation raw liquid;
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 80-85g, radix polygonati officinalis 75-85g, Fructus Corni 80-90g, Herba Cistanches 100-110g, Herba Epimedii 15-30g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted;
Step 3: obtain finished product after blending 58 ~ 62 degree of former base white wine of pure grain brew
Be the filling acquisition finished product of homogeneous after 50:1:49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 58 ~ 62 degree of pure grain brew former base white wine according to mass ratio.
Invention further provides a kind of preparation method of health care health cordyceps militaris liquor, comprise the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 6 days at 25 DEG C;
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium and weight as follows: 200 grams, potato, glucose 20 grams, peptone 3 grams, yeast extract paste 1 gram, 0.5 gram, magnesium sulfate, potassium primary phosphate 1 gram, nature pH value, incubation time 38 hours at 24 DEG C, wherein said liquid nutrient medium is in 1000ml;
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating;
Wherein corresponding one-level, secondary, three grades of submerged fermentation culture mediums are all identical, and its composition and weight are: millet 30g, glucose 20g, white sugar 2g, potassium dihydrogen sulfate 0.3g, magnesium sulfate 0.12g, peptone 4g, yeast extract 1g, amino acid 0.15g, vitamin V B10.01g, adds water to 1000ml;
Its process is as follows: with 10% inoculum size access liquid spawn, at 22 DEG C, under the condition that ventilating ratio 1:0.5 and the interior pressure of cultivation are 0.05MPa, continuously ferment 38 hours, now PH is 4.5, mycelium natural subsidence 100%, in aforementioned process, the condition of one-level, secondary, three grades of submerged fermentations is all identical;
D. active bacteria somatocyte broken wall
By live body bacterium liquid through the grinding of 3T/H colloidal mill, then use 3T/H, processing pressure is the high pressure homogenizer fracturing cell walls of 40MPa, forms suspendible glue, is incubated placement 2 hours, intracellular matter is fully oozed out, obtain Cordyceps mycelium fermenation raw liquid at 37 DEG C;
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 80g, radix polygonati officinalis 75g, Fructus Corni 80g, Herba Cistanches 110g, Herba Epimedii 15g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted;
Step 3: obtain finished product after blending 60 degree of former base white wine of pure grain brew
Be the filling acquisition finished product of homogeneous after 50:1:49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine according to mass ratio.
Invention further provides a kind of preparation method of health care health cordyceps militaris liquor, comprise the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 7 days at 25 DEG C;
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium and weight as follows: 220 grams, potato, glucose 18 grams, peptone 2 grams, yeast extract paste 1.5 grams, 0.6 gram, magnesium sulfate, potassium primary phosphate 0.5 gram, nature pH value, incubation time 42 hours at 25 DEG C, wherein said liquid nutrient medium is in 1000ml;
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating;
Wherein corresponding one-level, secondary, three grades of submerged fermentation culture mediums are all identical, and its composition and weight are: millet 35g, glucose 22g, white sugar 1g, potassium dihydrogen sulfate 1.2g, magnesium sulfate 0.1g, peptone 3g, yeast extract 2g, amino acid 0.1g, vitamin V B10.01g, adds water to 1000ml;
Its process is as follows: with 8% inoculum size access liquid spawn, at 23 DEG C, under the condition that ventilating ratio 1:0.6 and the interior pressure of cultivation are 0.04MPa, continuously ferment 36 hours, now PH is 4.8, mycelium natural subsidence 100%, and in aforementioned process, the condition of one-level, secondary, three grades of submerged fermentations is all identical;
D. active bacteria somatocyte broken wall
By live body bacterium liquid through the grinding of 3T/H colloidal mill, then use 3T/H, processing pressure is the high pressure homogenizer fracturing cell walls of 40MPa, form suspendible glue, at 35 DEG C, be incubated placement 1.5 hours, intracellular matter is fully oozed out, obtain Cordyceps mycelium fermenation raw liquid;
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 83g, radix polygonati officinalis 85g, Fructus Corni 90g, Herba Cistanches 100g, Herba Epimedii 20g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted;
Step 3: obtain finished product after blending 60 degree of former base white wine of pure grain brew
Be the canned acquisition finished product of homogeneous after 50:1:49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine according to mass ratio.
Invention further provides a kind of preparation method of health care health cordyceps militaris liquor, comprise the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 6 days at 23 DEG C;
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium and weight as follows: 180 grams, potato, glucose 18 grams, peptone 3 grams, yeast extract paste 1 gram, 0.5 gram, magnesium sulfate, potassium primary phosphate 1.5 grams, nature pH value, incubation time 36 hours at 24 DEG C, wherein said liquid nutrient medium is in 1000ml;
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating;
Wherein corresponding one-level, secondary, three grades of submerged fermentation culture mediums are all identical, and its composition and weight are: millet 32g, glucose 25g, white sugar 1.5g, potassium dihydrogen sulfate 0.8g, magnesium sulfate 0.15g, peptone 5g, yeast extract 1.5g, amino acid 0.2g, vitamin V B10.01g, adds water to 1000ml;
Its process is as follows: with 9% inoculum size access liquid spawn, at 22 DEG C, under the condition that ventilating ratio 1:0.7 and the interior pressure of cultivation are 0.03MPa, continuously ferment 37 hours, now PH is 5.0, mycelium natural subsidence 100%, and in aforementioned process, the condition of one-level, secondary, three grades of submerged fermentations is all identical;
D. active bacteria somatocyte broken wall
By live body bacterium liquid through the grinding of 3T/H colloidal mill, then use 3T/H, processing pressure is the high pressure homogenizer fracturing cell walls of 40MPa, form suspendible glue, at 40 DEG C, be incubated placement 1.5 hours, intracellular matter is fully oozed out, obtain Cordyceps mycelium fermenation raw liquid;
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 85g, radix polygonati officinalis 80g, Fructus Corni 85g, Herba Cistanches 105g, Herba Epimedii 30g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted;
Step 3: obtain finished product after blending 60 degree of former base white wine of pure grain brew
Be the canned acquisition finished product of homogeneous after 50:1:49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine according to mass ratio.
Invention further provides a kind of preparation method of health care health cordyceps militaris liquor, comprise the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 7 days at 25 DEG C;
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium and weight as follows: 200 grams, potato, glucose 22 grams, peptone 4 grams, yeast extract paste 0.5 gram, 0.6 gram, magnesium sulfate, potassium primary phosphate 1.2 grams, nature pH value, incubation time 40 hours at 23 DEG C, wherein said liquid nutrient medium is in 1000ml;
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating;
Wherein corresponding one-level, secondary, three grades of submerged fermentation culture mediums are all identical, and its composition and weight are: millet 34g, glucose 24g, white sugar 1g, potassium dihydrogen sulfate 1g, magnesium sulfate 0.1g, peptone 3.5g, yeast extract 1.8g, amino acid 0.12g, vitamin V B10.01g, adds water to 1000ml;
Its process is as follows: with 10% inoculum size access liquid spawn, at 23 DEG C, under the condition that ventilating ratio 1:0.6 and the interior pressure of cultivation are 0.05MPa, continuously ferment 37 hours, now PH is 4.6, mycelium natural subsidence 100%, in aforementioned process, the condition of one-level, secondary, three grades of submerged fermentations is all identical;
D. active bacteria somatocyte broken wall
By live body bacterium liquid through the grinding of 3T/H colloidal mill, then use 3T/H, processing pressure is the high pressure homogenizer fracturing cell walls of 40MPa, form suspendible glue, at 39 DEG C, be incubated placement 1.5 hours, intracellular matter is fully oozed out, obtain Cordyceps mycelium fermenation raw liquid;
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 82g, radix polygonati officinalis 82g, Fructus Corni 80g, Herba Cistanches 108g, Herba Epimedii 18g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted;
Step 3: obtain finished product after blending 60 degree of former base white wine of pure grain brew
Be the filling acquisition finished product of homogeneous after 50:1:49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine according to mass ratio.
In the extracts of Chinese herbal medicine that health care health cordyceps militaris liquor of the present invention uses, raw material used is mainly used in nourishing Yin and promoting production of body fluid the tuber of dwarf lilyturf, effect that moistening lung clears away heart-fire, cures mainly dryness of the lung dry cough, consumptive disease cough, thirsty, the vexed insomnia of Tianjin wound, interior heat is quenched one's thirst, the dry constipation of intestines and pharyngeal diphtheria; And radix polygonati officinalis has moistening lung enriching yin, effect of nourishing the stomach to promote the production of body fluid, cures mainly cough caused by dryness-heat, and consumptive disease is coughed for a long time; Consumption of YIN caused by febrile disease is thirsty, and interior heat is quenched one's thirst; Deficiency of Yin diseases caused by external factors, fever and chills are had a stuffy nose; The head is giddy, muscle arteries and veins contraction pain; Fructus Corni can tonify the liver and kidney, it is astringent or styptic treatment for spontaneous sweating to restrain, controlling nocturnal emission with astringent drugs contracting urine, only band only collapse, hidroschesis, promote the production of body fluid to quench thirst, and can be used for menoxenia under soreness of waist and knee joint, dizziness and tinnitus, forgetful, emission and spermatorrhea, enuresis frequent micturition, uterine bleeding band, profuse sweating collapse and interior heat and quenches one's thirst; Herba Cistanches can kidney-replenishing, and benefiting essence-blood, relaxes bowel, for impotence, infertile, soreness of the waist and knees, and muscles and bones is unable, the dry constipation of intestines.Kidney-replenishing, strengthening the bones and muscles, the diseases such as wind-damp dispelling; Herba Epimedii is used for that impotence and seminal emission, muscles and bones impotence are soft, rheumatic arthralgia, numbness contracture and climacteric hypertension.Adopt and use Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white spirit mixing finished products, wherein Cordyceps mycelium fermenation raw liquid is employing specifically fermentation technology, obtain the whole beer that fermentation thalli stoste is lumped together, carry out cytoclasis again, concentrate and be equipped with several Chinese medicine and modulate, both natural cordyceps effective ingredient can be kept, play special medical health-care effect, again can production control, improve output, reduce costs, meet industrial development direction, be applicable to promoting.
The present invention is separated from natural cordyceps and obtains meeting industrialization; the excellent cordyceps species of large-scale production; adopt modern deep layer cells circulation fermentation technique; obtain the ecosystem fermentation magma that fermented cordyceps hypha body magma is lumped together; carry out cytoclasis again; concentrate and be equipped with several Chinese medicine and modulate; both natural cordyceps effective active components can be kept; play special medical health-care effect; finally with the former base white spirit mixing of pure grain brew; " efficacy enhanced by wine, wine helps the efficacy of a drug " wine and medicine have synergy, improve drug effect.Wine is exactly a kind of good organic solvents, and its main component ethanol has good penetrance.Be easy to enter histocyte, medicine is played a role rapidly.Wine good stability simultaneously, and have the effect of corrosion-proof and sterilization, low alcohol is suitable for the preservation of the activeconstituents of Cordyceps mycelium, there are smooth blood vessels, effect of promoting blood circulation to remove blood stasis, the metabolism syndrome of Chinese caterpillar fungus original plasm wine to blood sugar, blood fat, blood uric acid finally obtained has auxiliary adjustment effect, can improve the complication of diabetes, as somnopathy, enuresis nocturna frequency, fatigue, skin pruritus etc. take effect, effective to vitiligo.Can produce by standardization control again, improve output, solve Cordyceps Resources problem, meet industrial development direction.
The present invention innovates at culture process formula, and the past is the biological medium of preparation, and only consider the vegetative propagation of microorganism, do not consider the functional effect of standardized product, in the compatibility of product, medium-height grass Extraction parts, only considers follow-up preparation.Invention has been adjustment, when making biological medium, Chinese herbal medicine extracting part, also as substratum, facts have proved, greatly remarkable in product curative effect, sufficient proof dissociant is quite abundant, is more conducive to the absorption of human body.
When the fermention medium that the present invention is unique in submerged fermentation enlarged culturing starts to cultivate, nature pH value is 6.8, culture cycle 36-38 hour, terminal pH value is 4.5-5.0, compare nature pH value be 6.2 substratum, culture cycle be 36-38 hour and terminal pH value is 3.6-3.8 time, each effective active component content can improve 10%, and biological transformation ratio improves 20%, solid content also improves 10%, more can give full play to the pharmaceutical use of Chinese caterpillar fungus.
Embodiment
The invention provides a kind of health care health cordyceps militaris liquor, is adopt Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 58 ~ 62 degree of pure grain brew former base white wine formulated; Described Cordyceps mycelium fermenation raw liquid carries out vegetative deep fermentation technology to Cordyceps strain to make; Described extracts of Chinese herbal medicine is extracted by the tuber of dwarf lilyturf, radix polygonati officinalis, Fructus Corni, Herba Cistanches and Herba Epimedii to make; The weight ratio of described Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 58 ~ 62 degree of former base white wine of pure grain brew is: 50:1:49.
The preparation method of described health care health cordyceps militaris liquor, comprises the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 5 ~ 7 days at 23 ~ 25 DEG C.
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium (in 1000ml) and weight as follows: 180 ~ 220 grams, potato, glucose 18 ~ 22 grams, peptone 2 ~ 4 grams, yeast extract paste 0.5 ~ 1.5 gram, 0.4 ~ 0.6 gram, magnesium sulfate, potassium primary phosphate 0.5 ~ 1.5 gram, natural pH value, incubation time 36 ~ 42 hours at 23 ~ 25 DEG C.
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating.
Wherein fermention medium (substratum of one-level, secondary, three grades of submerged fermentations is all identical) composition and weight are: millet 30-35g, glucose 20-25g, white sugar 1-2g, potassium dihydrogen sulfate 0.3-1.2g, magnesium sulfate 0.1-0.15g, peptone 3-5g, yeast extract 1-2g, amino acid 0.1-0.2g, vitamin V B10.005-0.015g, adds water to 1000ml.
Its process following (condition of one-level, secondary, three grades of submerged fermentations is all identical): with 8-10% inoculum size access liquid spawn, at 22-23 DEG C, under the condition that ventilating ratio 1:0.5 ~ 0.7 and the interior pressure of cultivation are 0.03-0.05MPa, continuously ferment 36-38 hour, now PH is 4.5 ~ 5.0, mycelium natural subsidence 100%.
D. active bacteria somatocyte broken wall
By live body bacterium liquid through colloidal mill grinding (3T/H), use high pressure homogenizer (3T/H again, processing pressure 40MPa) fracturing cell walls, form suspendible glue, placement is incubated 1 ~ 2 hour at 32 ~ 42 DEG C, intracellular matter is fully oozed out, obtains Cordyceps mycelium fermenation raw liquid.
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 80-85g, radix polygonati officinalis 75-85g, Fructus Corni 80-90g, Herba Cistanches 100-110g, Herba Epimedii 15-30g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted.
Step 3: obtain finished product after blending 58 ~ 62 degree of former base white wine of pure grain brew
Be the filling acquisition finished product of homogeneous after 50-52:1-2:47-49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 58 ~ 62 degree of pure grain brew former base white wine according to mass ratio.
Instructions of taking: morning, ante cibum, half an hour drank, be conducive to the absorption completely of medicine, during 5-7 in morning, kidney Liver Channel is the most active.First half hour of liking to sleep most evening is drunk, and can promote sleep like this, can also improve volume of blood flow and flow velocity at night, ensures blood oxygen supply heart and brain.Drink into ethanol 90% at liver internal oxidition, all the other are in lung and renal excretion, normal people's energy metabolism per hour 7-10 grams of ethanol, and ethanol dehydrogenase is converted into rapidly acetaldehyde → ethanol → tricarboxylic acid cycle (TCA) → CO2H2O.Data according to " Chinese food component list 2002 " calculate note, health care health cordyceps militaris liquor only 28 degree, every day 2 liang (100ml), drink at twice, once about containing ethanol 14 grams, every gram of ethanol generate energy 7 kilocalories, every day, production capacity about 200 kilocalories, will subtract staple food grain one or two (about 25 grams) every day.The elderly's individual difference is very large, as age, the course of disease.Basic health, complication and complication, the amount of drinking takes the circumstances into consideration increase and decrease, holds a little liquor and repeatedly can divide drink on a small quantity.
Now the present invention is further described in conjunction with the embodiments, but the invention is not restricted to following examples.
Embodiment 1
Present embodiments provide a kind of preparation method of health care health cordyceps militaris liquor, comprise the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 6 days at 25 DEG C.
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium (in 1000ml) and weight as follows: 200 grams, potato, glucose 20 grams, peptone 3 grams, yeast extract paste 1 gram, 0.5 gram, magnesium sulfate, potassium primary phosphate 1 gram, natural pH value, incubation time 38 hours at 24 DEG C.
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating.
Wherein fermention medium (substratum of one-level, secondary, three grades of submerged fermentations is all identical) composition and weight are: millet 30g, glucose 20g, white sugar 2g, potassium dihydrogen sulfate 0.3g, magnesium sulfate 0.12g, peptone 4g, yeast extract 1g, amino acid 0.15g, vitamin V B10.01g, adds water to 1000ml.
Its process following (condition of one-level, secondary, three grades of submerged fermentations is all identical): with 10% inoculum size access liquid spawn, at 22 DEG C, under the condition that ventilating ratio 1:0.5 and the interior pressure of cultivation are 0.05MPa, continuously ferment 38 hours, now PH is 4.5, mycelium natural subsidence 100%.
D. active bacteria somatocyte broken wall
By live body bacterium liquid through colloidal mill grinding (3T/H), then use high pressure homogenizer (3T/H, processing pressure 40MPa) fracturing cell walls, form suspendible glue, at 37 DEG C, be incubated placement 2 hours, intracellular matter is fully oozed out, obtain Cordyceps mycelium fermenation raw liquid.
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 80g, radix polygonati officinalis 75g, Fructus Corni 80g, Herba Cistanches 110g, Herba Epimedii 15g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted.
Step 3: obtain finished product after blending 60 degree of former base white wine of pure grain brew
Be the filling acquisition finished product of homogeneous after 50:1:49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine according to mass ratio.
Embodiment 2
Present embodiments providing a kind of health care health cordyceps militaris liquor, is adopt Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine formulated.
The preparation method of described health care health cordyceps militaris liquor, comprises the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 7 days at 25 DEG C.
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium (in 1000ml) and weight as follows: 220 grams, potato, glucose 18 grams, peptone 2 grams, yeast extract paste 1.5 grams, 0.6 gram, magnesium sulfate, potassium primary phosphate 0.5 gram, natural pH value, incubation time 42 hours at 25 DEG C.
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating.
Wherein fermention medium (substratum of one-level, secondary, three grades of submerged fermentations is all identical) composition and weight are: millet 35g, glucose 22g, white sugar 1g, potassium dihydrogen sulfate 1.2g, magnesium sulfate 0.1g, peptone 3g, yeast extract 2g, amino acid 0.1g, vitamin V B10.01g, adds water to 1000ml.
Its process following (condition of one-level, secondary, three grades of submerged fermentations is all identical): with 8% inoculum size access liquid spawn, at 23 DEG C, under the condition that ventilating ratio 1:0.6 and the interior pressure of cultivation are 0.04MPa, continuously ferment 36 hours, now PH is 4.8, mycelium natural subsidence 100%.
D. active bacteria somatocyte broken wall
By live body bacterium liquid through colloidal mill grinding (3T/H), then use high pressure homogenizer (3T/H, processing pressure 40MPa) fracturing cell walls, form suspendible glue, at 35 DEG C, be incubated placement 1.5 hours, intracellular matter is fully oozed out, obtain Cordyceps mycelium fermenation raw liquid.
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 83g, radix polygonati officinalis 85g, Fructus Corni 90g, Herba Cistanches 100g, Herba Epimedii 20g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted.
Step 3: obtain finished product after blending 60 degree of former base white wine of pure grain brew
Be the canned acquisition finished product of homogeneous after 50:1:49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine according to mass ratio.
Embodiment 3
Present embodiments providing a kind of health care health cordyceps militaris liquor, is adopt Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine formulated.
The preparation method of described health care health cordyceps militaris liquor, comprises the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 6 days at 23 DEG C.
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium (in 1000ml) and weight as follows: 180 grams, potato, glucose 18 grams, peptone 3 grams, yeast extract paste 1 gram, 0.5 gram, magnesium sulfate, potassium primary phosphate 1.5 grams, natural pH value, incubation time 36 hours at 24 DEG C.
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating.
Wherein fermention medium (substratum of one-level, secondary, three grades of submerged fermentations is all identical) composition and weight are: millet 32g, glucose 25g, white sugar 1.5g, potassium dihydrogen sulfate 0.8g, magnesium sulfate 0.15g, peptone 5g, yeast extract 1.5g, amino acid 0.2g, vitamin V B10.01g, adds water to 1000ml.
Its process following (condition of one-level, secondary, three grades of submerged fermentations is all identical): with 9% inoculum size access liquid spawn, at 22 DEG C, under the condition that ventilating ratio 1:0.7 and the interior pressure of cultivation are 0.03MPa, continuously ferment 37 hours, now PH is 5.0, mycelium natural subsidence 100%.
D. active bacteria somatocyte broken wall
By live body bacterium liquid through colloidal mill grinding (3T/H), then use high pressure homogenizer (3T/H, processing pressure 40MPa) fracturing cell walls, form suspendible glue, at 40 DEG C, be incubated placement 1.5 hours, intracellular matter is fully oozed out, obtain Cordyceps mycelium fermenation raw liquid.
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 85g, radix polygonati officinalis 80g, Fructus Corni 85g, Herba Cistanches 105g, Herba Epimedii 30g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted.
Step 3: obtain finished product after blending 60 degree of former base white wine of pure grain brew
Be the canned acquisition finished product of homogeneous after 50:1:49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine according to mass ratio.
Embodiment 4
The invention provides a kind of health care health cordyceps militaris liquor, is adopt Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine formulated.
The preparation method of described health care health cordyceps militaris liquor, comprises the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 7 days at 25 DEG C.
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium (in 1000ml) and weight as follows: 200 grams, potato, glucose 22 grams, peptone 4 grams, yeast extract paste 0.5 gram, 0.6 gram, magnesium sulfate, potassium primary phosphate 1.2 grams, natural pH value, incubation time 40 hours at 23 DEG C.
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating.
Wherein fermention medium (substratum of one-level, secondary, three grades of submerged fermentations is all identical) composition and weight are: millet 34g, glucose 24g, white sugar 1g, potassium dihydrogen sulfate 1g, magnesium sulfate 0.1g, peptone 3.5g, yeast extract 1.8g, amino acid 0.12g, vitamin V B10.01g, adds water to 1000ml.
Its process following (condition of one-level, secondary, three grades of submerged fermentations is all identical): with 10% inoculum size access liquid spawn, at 23 DEG C, under the condition that ventilating ratio 1:0.6 and the interior pressure of cultivation are 0.05MPa, continuously ferment 37 hours, now PH is 4.6, mycelium natural subsidence 100%.
D. active bacteria somatocyte broken wall
By live body bacterium liquid through colloidal mill grinding (3T/H), then use high pressure homogenizer (3T/H, processing pressure 40MPa) fracturing cell walls, form suspendible glue, at 39 DEG C, be incubated placement 1.5 hours, intracellular matter is fully oozed out, obtain Cordyceps mycelium fermenation raw liquid.
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 82g, radix polygonati officinalis 82g, Fructus Corni 80g, Herba Cistanches 108g, Herba Epimedii 18g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted.
Step 3: obtain finished product after blending 60 degree of former base white wine of pure grain brew
Be the filling acquisition finished product of homogeneous after 50:1:49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine according to mass ratio.
Health care health cordyceps militaris liquor of the present invention, through Institute of Microorganism, Academia Sinica, authoritative institution's composition detection such as microbiological analysis inspection center of Guangdong Province confirm that it contains ucleosides (adenosine), N.F,USP MANNITOL (cordycepic acid), cordycepin, Cordyceps polysaccharide and 20 multiple amino acids, wherein detect 7 kinds and (must refer to that human body self can not synthesize by amino acid, must from the amino acid of ingestion of food), be hydrolyzed or total free aminoacids qualification all in accreditation scope, also has superoxide dismutase, the bioactive ingredients such as trace element are all up to standard, do not measure hazardous and noxious substances.The Pharmacopoeia of the People's Republic of China (version in 2005) is decided to be adenosine the assay project of Cordyceps sinensis, regulation Chinese caterpillar fungus adenosine must not be less than 0.010%, and in health care health cordyceps militaris liquor of the present invention after testing adenosine be 0.615mg/500ml (for embodiment 3), it is 60 times of natural cordyceps, biological polyoses and cordycepin are 30 times of natural cordyceps, detect in addition human body healthy trace elements with household.
Detection method:
Participate in experience number 27 people, wherein the male sex 14 example, women 13 example; Max age 83 years old, minimum 30 years old.The T2DM disease that is diagnosed as wherein meeting WHO diabetes diagnostic criterion in 1999 has 21 people, and the course of disease is the longest 23 years, the shortest one week, also has 6 routine sub-health populations, the wherein male sex 5 people, women 1 people.
Experience patient to participate in application voluntarily, the principle of the two-way choice of expert's approval.Use hypoglycemic oral medicine or insulin injection person, all maintained original scheme in the past.Give health care health cordyceps militaris liquor (28 degree of Chinese caterpillar fungus original plasm wines) every day 2 times prepared by the embodiment of the present invention 1 on this basis.Each 50ml (about 1 liang) requirement, sooner or later respectively once.The people held a little liquor can repeatedly drink in right amount, within 21 days, experiences and terminates.
Arrive first free Grade A hospital before drinking to draw blood on an empty stomach detection; Glycated serum protein (GSD), total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL) (HDL), low-density lipoprotein (LDL), blood uric acid (UA), check oneself the projects such as fasting plasma glucose (FPG), after the meal 2 hours blood glucoses (P2HBG), blood pressure and subjective symptoms.Within 21 days, experience after terminating.Namely arrive the project that former Grade A hospital duplicate detection is same, with drink before contrast and carry out self-contrast.And fill in from tabling look-up acceptance.
Detected result
Detected result is as shown in table 1, table 2 and table 3.
Table 1
As can be seen from Table 1, blood sugar test person is all the diabetes B patients made a definite diagnosis, and total effective rate is 44%.Wherein 4 people urinate hyperglycemic patients and decline by a big margin, and other is slightly stable declines or raises.
Table 2
As can be seen from Table 2, four items of blood lipid tests heave amplitude is remarkable, and high-density lipoprotein (HDL) rises 84.6%, and low-density lipoprotein declines 57.7%, and total effective rate is 59.4%.
Table 3
As can be seen from Table 3, it is effectively 62.3% that subjective symptoms is always improved, and subjective symptoms mostly is the complication of diabetes, and sleep quality improves enuresis nocturna and reduces, and energy muscle power is improved.
Claims (5)
1. a preparation method for health care health cordyceps militaris liquor, is characterized in that, comprises the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 5 ~ 7 days at 23 ~ 25 DEG C;
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium and weight as follows: 180 ~ 220 grams, potato, glucose 18 ~ 22 grams, peptone 2 ~ 4 grams, yeast extract paste 0.5 ~ 1.5 gram, 0.4 ~ 0.6 gram, magnesium sulfate, potassium primary phosphate 0.5 ~ 1.5 gram, natural pH value, incubation time 36 ~ 42 hours at 23 ~ 25 DEG C, wherein said liquid nutrient medium is in 1000ml;
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating;
Wherein corresponding one-level, secondary, three grades of submerged fermentation culture mediums are all identical, its composition and weight are: millet 30-35g, glucose 20-25g, white sugar 1-2g, potassium dihydrogen sulfate 0.3-1.2g, magnesium sulfate 0.1-0.15g, peptone 3-5g, yeast extract 1-2g, amino acid 0.1-0.2g, vitamin V B10.005-0.015g, adds water to 1000ml;
Its process is as follows: with 8-10% inoculum size access liquid spawn, at 22-23 DEG C, under the condition that ventilating ratio 1:0.5 ~ 0.7 and the interior pressure of cultivation are 0.03-0.05MPa, continuously ferment 36-38 hour, now PH is 4.5 ~ 5.0, mycelium natural subsidence 100%, in aforementioned process, the condition of one-level, secondary, three grades of submerged fermentations is all identical;
D. active bacteria somatocyte broken wall
By live body bacterium liquid through the grinding of 3T/H colloidal mill, then use 3T/H, processing pressure is the high pressure homogenizer fracturing cell walls of 40MPa, form suspendible glue, at 32 ~ 42 DEG C, be incubated placement 1 ~ 2 hour, intracellular matter is fully oozed out, obtain Cordyceps mycelium fermenation raw liquid;
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 80-85g, radix polygonati officinalis 75-85g, Fructus Corni 80-90g, Herba Cistanches 100-110g, Herba Epimedii 15-30g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted;
Step 3: obtain finished product after blending 58 ~ 62 degree of former base white wine of pure grain brew
Be the filling acquisition finished product of homogeneous after 50:1:49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 58 ~ 62 degree of pure grain brew former base white wine according to mass ratio.
2. the preparation method of health care health cordyceps militaris liquor according to claim 1, is characterized in that, comprise the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 6 days at 25 DEG C;
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium and weight as follows: 200 grams, potato, glucose 20 grams, peptone 3 grams, yeast extract paste 1 gram, 0.5 gram, magnesium sulfate, potassium primary phosphate 1 gram, nature pH value, incubation time 38 hours at 24 DEG C, wherein said liquid nutrient medium is in 1000ml;
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating;
Wherein corresponding one-level, secondary, three grades of submerged fermentation culture mediums are all identical, and its composition and weight are: millet 30g, glucose 20g, white sugar 2g, potassium dihydrogen sulfate 0.3g, magnesium sulfate 0.12g, peptone 4g, yeast extract 1g, amino acid 0.15g, vitamin V B10.01g, adds water to 1000ml;
Its process is as follows: with 10% inoculum size access liquid spawn, at 22 DEG C, under the condition that ventilating ratio 1:0.5 and the interior pressure of cultivation are 0.05MPa, continuously ferment 38 hours, now PH is 4.5, mycelium natural subsidence 100%, in aforementioned process, the condition of one-level, secondary, three grades of submerged fermentations is all identical;
D. active bacteria somatocyte broken wall
By live body bacterium liquid through the grinding of 3T/H colloidal mill, then use 3T/H, processing pressure is the high pressure homogenizer fracturing cell walls of 40MPa, forms suspendible glue, is incubated placement 2 hours, intracellular matter is fully oozed out, obtain Cordyceps mycelium fermenation raw liquid at 37 DEG C;
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 80g, radix polygonati officinalis 75g, Fructus Corni 80g, Herba Cistanches 110g, Herba Epimedii 15g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted;
Step 3: obtain finished product after blending 60 degree of former base white wine of pure grain brew
Be the filling acquisition finished product of homogeneous after 50:1:49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine according to mass ratio.
3. the preparation method of health care health cordyceps militaris liquor according to claim 1, is characterized in that, comprise the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 7 days at 25 DEG C;
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium and weight as follows: 220 grams, potato, glucose 18 grams, peptone 2 grams, yeast extract paste 1.5 grams, 0.6 gram, magnesium sulfate, potassium primary phosphate 0.5 gram, nature pH value, incubation time 42 hours at 25 DEG C, wherein said liquid nutrient medium is in 1000ml;
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating;
Wherein corresponding one-level, secondary, three grades of submerged fermentation culture mediums are all identical, and its composition and weight are: millet 35g, glucose 22g, white sugar 1g, potassium dihydrogen sulfate 1.2g, magnesium sulfate 0.1g, peptone 3g, yeast extract 2g, amino acid 0.1g, vitamin V B10.01g, adds water to 1000ml;
Its process is as follows: with 8% inoculum size access liquid spawn, at 23 DEG C, under the condition that ventilating ratio 1:0.6 and the interior pressure of cultivation are 0.04MPa, continuously ferment 36 hours, now PH is 4.8, mycelium natural subsidence 100%, and in aforementioned process, the condition of one-level, secondary, three grades of submerged fermentations is all identical;
D. active bacteria somatocyte broken wall
By live body bacterium liquid through the grinding of 3T/H colloidal mill, then use 3T/H, processing pressure is the high pressure homogenizer fracturing cell walls of 40MPa, form suspendible glue, at 35 DEG C, be incubated placement 1.5 hours, intracellular matter is fully oozed out, obtain Cordyceps mycelium fermenation raw liquid;
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 83g, radix polygonati officinalis 85g, Fructus Corni 90g, Herba Cistanches 100g, Herba Epimedii 20g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted;
Step 3: obtain finished product after blending 60 degree of former base white wine of pure grain brew
Be the canned acquisition finished product of homogeneous after 50:1:49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine according to mass ratio.
4. the preparation method of health care health cordyceps militaris liquor according to claim 1, is characterized in that, comprise the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 6 days at 23 DEG C;
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium and weight as follows: 180 grams, potato, glucose 18 grams, peptone 3 grams, yeast extract paste 1 gram, 0.5 gram, magnesium sulfate, potassium primary phosphate 1.5 grams, nature pH value, incubation time 36 hours at 24 DEG C, wherein said liquid nutrient medium is in 1000ml;
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating;
Wherein corresponding one-level, secondary, three grades of submerged fermentation culture mediums are all identical, and its composition and weight are: millet 32g, glucose 25g, white sugar 1.5g, potassium dihydrogen sulfate 0.8g, magnesium sulfate 0.15g, peptone 5g, yeast extract 1.5g, amino acid 0.2g, vitamin V B10.01g, adds water to 1000ml;
Its process is as follows: with 9% inoculum size access liquid spawn, at 22 DEG C, under the condition that ventilating ratio 1:0.7 and the interior pressure of cultivation are 0.03MPa, continuously ferment 37 hours, now PH is 5.0, mycelium natural subsidence 100%, and in aforementioned process, the condition of one-level, secondary, three grades of submerged fermentations is all identical;
D. active bacteria somatocyte broken wall
By live body bacterium liquid through the grinding of 3T/H colloidal mill, then use 3T/H, processing pressure is the high pressure homogenizer fracturing cell walls of 40MPa, form suspendible glue, at 40 DEG C, be incubated placement 1.5 hours, intracellular matter is fully oozed out, obtain Cordyceps mycelium fermenation raw liquid;
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 85g, radix polygonati officinalis 80g, Fructus Corni 85g, Herba Cistanches 105g, Herba Epimedii 30g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted;
Step 3: obtain finished product after blending 60 degree of former base white wine of pure grain brew
Be the canned acquisition finished product of homogeneous after 50:1:49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine according to mass ratio.
5. the preparation method of health care health cordyceps militaris liquor according to claim 1, is characterized in that, comprise the steps:
Step one. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, slant medium is cultivated, obtained slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 7 days at 25 DEG C;
B. the preparation of liquid spawn
Cultivate in the inclined-plane solid spawn liquid medium within getting above-mentioned cultivation, obtained liquid spawn; Wherein the composition of liquid nutrient medium and weight as follows: 200 grams, potato, glucose 22 grams, peptone 4 grams, yeast extract paste 0.5 gram, 0.6 gram, magnesium sulfate, potassium primary phosphate 1.2 grams, nature pH value, incubation time 40 hours at 23 DEG C, wherein said liquid nutrient medium is in 1000ml;
C. submerged fermentation enlarged culturing
Liquid spawn aseptically being accessed successively in the one-level of the nutrient solution filled through sterilising treatment, secondary, three grades of Submerged fermentation devices, obtaining live body bacterium liquid through cultivating;
Wherein corresponding one-level, secondary, three grades of submerged fermentation culture mediums are all identical, and its composition and weight are: millet 34g, glucose 24g, white sugar 1g, potassium dihydrogen sulfate 1g, magnesium sulfate 0.1g, peptone 3.5g, yeast extract 1.8g, amino acid 0.12g, vitamin V B10.01g, adds water to 1000ml;
Its process is as follows: with 10% inoculum size access liquid spawn, at 23 DEG C, under the condition that ventilating ratio 1:0.6 and the interior pressure of cultivation are 0.05MPa, continuously ferment 37 hours, now PH is 4.6, mycelium natural subsidence 100%, in aforementioned process, the condition of one-level, secondary, three grades of submerged fermentations is all identical;
D. active bacteria somatocyte broken wall
By live body bacterium liquid through the grinding of 3T/H colloidal mill, then use 3T/H, processing pressure is the high pressure homogenizer fracturing cell walls of 40MPa, form suspendible glue, at 39 DEG C, be incubated placement 1.5 hours, intracellular matter is fully oozed out, obtain Cordyceps mycelium fermenation raw liquid;
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 82g, radix polygonati officinalis 82g, Fructus Corni 80g, Herba Cistanches 108g, Herba Epimedii 18g, by above-mentioned Chinese medicine material with the water extraction secondary of 10 times amount, filters to obtain extracts of Chinese herbal medicine, it is extract 120 minutes at temperature 100 DEG C that first time extracts, and it is extract 60 minutes at 100 DEG C that second time is extracted;
Step 3: obtain finished product after blending 60 degree of former base white wine of pure grain brew
Be the filling acquisition finished product of homogeneous after 50:1:49 merges by above-mentioned obtained Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and 60 degree of pure grain brew former base white wine according to mass ratio.
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| CN105219598A (en) * | 2015-08-11 | 2016-01-06 | 深圳市藏虫草生物科技有限公司 | A kind of health care Cordyceps fungus wine and preparation method thereof |
| CN106635606A (en) * | 2015-11-03 | 2017-05-10 | 宿迁楚霸菌业有限公司 | Production process of cordyceps militaris health-preserving liquor |
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| CN110591929A (en) * | 2019-09-26 | 2019-12-20 | 大时代生物工程(深圳)有限公司 | Formula and production process of novel cordyceps sinensis health-care and health-preserving wine |
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| CN104547546A (en) * | 2014-12-26 | 2015-04-29 | 扬子江药业集团四川海蓉药业有限公司 | Traditional Chinese medicinal composition for reinforcing kidney and moistening lung and preparation method of traditional Chinese medicinal composition in different dosage forms |
| CN104547546B (en) * | 2014-12-26 | 2018-03-02 | 扬子江药业集团四川海蓉药业有限公司 | A kind of Chinese medicine composition of kidney tonifying moistening lung and its preparation method of different dosage forms |
| CN105219598A (en) * | 2015-08-11 | 2016-01-06 | 深圳市藏虫草生物科技有限公司 | A kind of health care Cordyceps fungus wine and preparation method thereof |
| CN106635606A (en) * | 2015-11-03 | 2017-05-10 | 宿迁楚霸菌业有限公司 | Production process of cordyceps militaris health-preserving liquor |
| CN107354071A (en) * | 2017-09-02 | 2017-11-17 | 深圳市善衍生物工程有限公司 | A kind of health care's Antrodia camphorata magma medicinal liquor and preparation method thereof |
| CN107435014A (en) * | 2017-09-02 | 2017-12-05 | 深圳市善衍生物工程有限公司 | A kind of health care's Antrodia camphorata magma oral liquid and preparation method thereof |
| CN110129168A (en) * | 2019-04-30 | 2019-08-16 | 李超飞 | A kind of Cordceps militaris prepares the culture medium prescription and preparation method of living Chinese caterpillar wine |
| CN110353140A (en) * | 2019-08-28 | 2019-10-22 | 大时代生物工程(深圳)有限公司 | A kind of health care's functionality cordyceps sinensis primary pulp drink and preparation method thereof |
| CN110591929A (en) * | 2019-09-26 | 2019-12-20 | 大时代生物工程(深圳)有限公司 | Formula and production process of novel cordyceps sinensis health-care and health-preserving wine |
| CN113214944A (en) * | 2021-05-07 | 2021-08-06 | 大时代生物工程(深圳)有限公司 | Novel cordyceps sinensis health-care health-preserving wine formula |
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Application publication date: 20141224 |