CN102523908A - Cordyceps militaris strain liquid amplification technology - Google Patents
Cordyceps militaris strain liquid amplification technology Download PDFInfo
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- CN102523908A CN102523908A CN2010105783758A CN201010578375A CN102523908A CN 102523908 A CN102523908 A CN 102523908A CN 2010105783758 A CN2010105783758 A CN 2010105783758A CN 201010578375 A CN201010578375 A CN 201010578375A CN 102523908 A CN102523908 A CN 102523908A
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- cordyceps militaris
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Abstract
The invention relates to a cordyceps militaris strain liquid amplification technology, belongs to the technical field of biology, solves the problems that the traditional production technology of the cordyceps militaris strain has long cycle and is easy to generate cross contamination, and invents the detection technology of the cordyceps militaris liquid strain to guarantee the quality of the amplified strain. The cordyceps militaris strain liquid amplification technology comprises the following steps of: (1). inoculating solid cordyceps militaris strains in a special liquid strain medium and cultivating the solid cordyceps militaris strains under certain conditions; (2). carrying out quality inspection on the strain liquid obtained by cultivating in the step (1); (3). carrying out amplification cultivation on excellent strains; (4). carrying out quality inspection on the amplified strains; and (5). adding sterile water in the excellent strains with the ratio of the excellent strains to the sterile water being 1:100, so as to obtain the cordyceps militaris liquid strain. And the cordyceps militaris strain liquid amplification technology is used for inoculating and cultivating cordyceps militaris fruit bodies.
Description
Technical field: biotechnology
Background technology: it is reported that the present stage cultivation of northern Chinese caterpillar Fungus all is through the amplification of solid mycelia, utilizes solid spawn to cultivate again.As: (the application (patent) number: 01128033.6) of northern Chinese caterpillar Fungus high-yield culturing and Chinese caterpillar fungus Processing Technique of New Products; (application (patent) number: the cultivation method of carrying all is the solid spawn cultivation to the new technology of artificially culturing north Chinese caterpillar fungus 92109225.3); (the application (patent) number: 200910103625.X) reported the liquid strain of cordyceps militaris production method of a kind of production method of liquid strain of cordyceps militaris; But its limitation is that experimental arrangement is various is easy to cross-infection; And it lacks checking procedure for the strain quality of producing, and can't guarantee strain quality.Present technique has been invented Coragyceps militaris bacterium liquid amplification technique; Accomplish the bacterial classification recycle, not only effectively reduce the cross-infection that traditional spawn culture method is taken place, and increased strain quality detection step; Guaranteed the strain quality after the amplification, for the cultivation of fruit body is laid a solid foundation.
Summary of the invention: 1, the object of the invention statement: invention Coragyceps militaris bacterium liquid amplification technique, reduce northern Chinese caterpillar Fungus cultivation pollution rate, shorten the northern Chinese caterpillar Fungus cultivation cycle.
2, detailed description of the present invention:
The first step: configuration bacterial classification liquid nutrient medium
Culture medium prescription: potassium dihydrogen phosphate 2.5g, epsom salt 3g, peptone 10g, glucose 300g, soluble starch 30g (fine flour, rice or potato) vitamin B1 (VB
1) 10mg, distilled water 1050ml.
The medium collocation method:
1) gets 1050ml water, boil;
2) treat that boiled water temperature drops to below 30 ℃ after, respectively soluble agents is added the people in the boiling water of boiling;
3) soluble starch with the furnishing pasty state adds the people slowly in aforesaid liquid;
4) with 5% hydrochloric acid or sodium hydroxide solution adjust pH to 6;
5) perhaps test in bottle at vial the above-mentioned liquid subpackage that configures;
6) liquid that branch is installed is sterilized, and sterilising conditions is 121 ℃, following 15 minutes of the pressure of 1.5P.
Second step: inoculation
1) sprays superclean bench with 75% the alcohol that configures, open the superclean bench uviol lamp then and sterilize.
2) after etc. the uviol lamp in the superclean bench is opened 15 minutes, the sterilization stove in the superclean bench is opened;
3) liquid nutrient medium that configures and northern Chinese caterpillar Fungus solid spawn and used tool are placed on the superclean bench table top of the bacterium of going out the smart spray disinfectant of serving a round of liquor to the guests of going forward side by side.
4) with tweezers the solid spawn mycelia is chosen the liquid culture primary surface, build bottle cap and get final product.
The 3rd step: culture environment
1) will inoculate good medium and be placed on the shaking table, it is 120-140r/s that shaking speed is set.
2) regulate environmental temperature to 20-25 ℃, humidity is to 80-90%
3) secretly cultivate 5-6 days, when seeing that medium is cotton-shaped, can stop cultivating.
The 4th step: bacterial classification detects
Optionally choose several bottles in the scattered light held.If 3 one 7d aerial hyphaes transfer golden yellow to, this shows that this bacterial classification kind property is good, solid very capable, can relievedly use, otherwise Ying Shenyong.
The 5th step: amplification
1) excellent species was inoculated into according to 1: 100 once more carries out amplification cultivation in the liquid nutrient medium.
2) repeat accurate the 4th of this step, the excellent species of picking out solid culture medium on was carried out entity cultivation with sterile water to converging, can inoculating by 1: 50.
3, the useful effect of the present invention: the present invention has not only shortened the cultivation cycle of northern Chinese caterpillar Fungus 1/3rd; And effectively avoided the cross-infection that taken place in the northern Chinese caterpillar Fungus cultivation process, help applying of technology when improving the cultivation efficient of northern Chinese caterpillar Fungus.
Embodiment: 1, select good solid spawn.
2, utilize the northern Chinese caterpillar Fungus solid spawn to carry out the liquid amplification.
3, the general selects good liquid spawn.
4, good liquid spawn is increased.
5, the bacterial classification after the amplification is carried out general's choosing.
6, with good liquid spawn and 1: 50 pair of remittance of sterile water, can the inoculated and cultured fruit body.
Claims (4)
- A kind of Coragyceps militaris bacterium liquid amplification technique; It is characterized by and invented Coragyceps militaris bacterium liquid amplification technique; Accomplish the bacterial classification recycle, not only effectively reduce the cross-infection that traditional spawn culture method is taken place, and increased strain quality detection step; Guaranteed the strain quality after the amplification, for the cultivation of fruit body is laid a solid foundation.Ask for protection scope:1. Coragyceps militaris bacterium liquid culture based formulas.
- 2. Coragyceps militaris bacterium liquid nutrient medium collocation method.
- 3. Coragyceps militaris bacterium liquid amplification technique culture environment.
- 4. liquid strain of cordyceps militaris detection technique.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105783758A CN102523908A (en) | 2010-12-08 | 2010-12-08 | Cordyceps militaris strain liquid amplification technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105783758A CN102523908A (en) | 2010-12-08 | 2010-12-08 | Cordyceps militaris strain liquid amplification technology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102523908A true CN102523908A (en) | 2012-07-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105783758A Pending CN102523908A (en) | 2010-12-08 | 2010-12-08 | Cordyceps militaris strain liquid amplification technology |
Country Status (1)
| Country | Link |
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| CN (1) | CN102523908A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1445360A (en) * | 2002-03-20 | 2003-10-01 | 中国科学院沈阳应用生态研究所 | Method of artificial planting northerly Chinese caterpillar fungus for increasing content of physiologically active substance |
| CN1807579A (en) * | 2006-01-23 | 2006-07-26 | 孟震 | Complex function type northern Chinese caterpillar fungus and its production method |
| CN1887049A (en) * | 2005-06-28 | 2007-01-03 | 兰著碧 | Aweto producing process |
-
2010
- 2010-12-08 CN CN2010105783758A patent/CN102523908A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1445360A (en) * | 2002-03-20 | 2003-10-01 | 中国科学院沈阳应用生态研究所 | Method of artificial planting northerly Chinese caterpillar fungus for increasing content of physiologically active substance |
| CN1887049A (en) * | 2005-06-28 | 2007-01-03 | 兰著碧 | Aweto producing process |
| CN1807579A (en) * | 2006-01-23 | 2006-07-26 | 孟震 | Complex function type northern Chinese caterpillar fungus and its production method |
Non-Patent Citations (2)
| Title |
|---|
| 崔颂英等: "北冬虫夏草高产栽培的几项有效措施", 《安徽农业》, no. 5, 31 December 2003 (2003-12-31) * |
| 杨淑全等: "冬虫夏草液体培养初报", 《中国食用菌》, no. 5, 31 December 1999 (1999-12-31) * |
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Addressee: Jie Junwen Document name: Notification that Application Deemed to be Withdrawn |
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Application publication date: 20120704 |
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