CN1887049A - Aweto producing process - Google Patents
Aweto producing process Download PDFInfo
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- CN1887049A CN1887049A CN 200510046800 CN200510046800A CN1887049A CN 1887049 A CN1887049 A CN 1887049A CN 200510046800 CN200510046800 CN 200510046800 CN 200510046800 A CN200510046800 A CN 200510046800A CN 1887049 A CN1887049 A CN 1887049A
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Abstract
The aweto producing process is one liquid culturing process with northeast China rice and/or corn meal as culture medium. The culturing process includes bottling the culture medium with 1.2-1.7 times water, heating to sterilize and cure, and cooling; inoculating aweto seed to the surface of the culture medium and sealing the bottle; growing in nature lighting at 16-20 deg.c and opening the bottle mouth to supply oxygen and lighting for 9-10 hr every day after the hypha becomes orange and needle shaped sporophore primordum appears; controlling the temperature in 17-19 deg.c and relative air humidity in 65-75 % during sporophore growth period; picking up after the sporophore become yellowish; and stoving in the initial temperature not higher than 25-30 deg.c and highest temperature not higher than 50 deg.c. The aweto product has cordycepin content up to 1.1 %, aweto polysaccharide content of 15.4 %, SOD enzyme of 386.36 ppm, total amino acid content of 30.51459 % and protein of 37.42 %.
Description
Technical field: aweto producing process is that Cordyceps militaris (or claiming northern Chinese caterpillar Fungus) carries out the production technology that cultivation is produced in batch production.
Background technology: " Cordyceps sinensis " is that traditional Chinese medicine is protected one of three big treasures in the storehouse, but wild resource is extremely scarce.Scientific research proves: " Cordyceps militaris " has the effective component similar to " Cordyceps sinensis ", can replace " Cordyceps sinensis " to be used as medicine.China's artificial cultivation research of " Cordyceps militaris " after this century the eighties obtains breakthrough development.But the artificial cultivation of existing " Cordyceps militaris " all is to adopt existing edible fungus culturing production method, though preferred northern Chinese caterpillar Fungus bacterial strain carries out strain cultivation.Liquid-spawn inoculation is cultivated on the medium of cultivation, because the arbitrariness of the material selection of culture medium for cultivating, mycelium formation phase adopt lucifuge, cultivation temperature to select 22-28 ℃ for use, make and produce instability, success rate is low, brings very big difficulty to the large-scale production cultivation.Strain cultivation is also very unstable.
Summary of the invention:
The present invention is exactly growth instability, the product active ingredient of producing and the lower technical problem of biologically active that solves in the existing aweto producing process, and the technology of a kind of artificial extensive anniversary producing northern Chinese caterpillar Fungus is provided.
In order to address the above problem, aweto producing process of the present invention, with preferred northern Chinese caterpillar Fungus bacterial strain, carry out strain cultivation, it is characterized in that: the medium of A. cultivation selects for use the good northeast rice of meter matter or corn short, bristly hair or beard or northeast rice and corn short, bristly hair or beard to mix, and packs in the bottle by the ratio of material-water ratio 1: 1.2-1.7, cools off after the slaking of sterilizing through heating, after liquid spawn is spilled into media surface under aseptic condition, with bottle closure; The bottle that B. will have medium and bacterial classification is put into the natural daylight cultivation down of not direct projection, temperature 16-20 ℃, the mycelia growth that begins to sprout is after the mycelia color becomes orange colour and has the needle-like fruit body primordium to occur, the oxygen therapy of abroaching has to reach 9-10 hour between the light time every day; Be controlled at 17-19 ℃ in whole sporophore growth phase temperature, relative air humidity 65-75%; C. Sheng Chang fruit-body color has reached the ripe phase by the deep orange look thin out is gradually when yellow, gathers, can not baking with 25-30 ℃ of maximum temperature of initial temperature above 50 ℃, and moisture to being lower than 5%.The medium of selecting for use is particularly suitable for northern Chinese caterpillar Fungus (Cordyceps militaris) growth.Whole incubation for " have light cultivate " with generally all to advocate " dark " cultivation in earlier stage different, since the stimulation of light make Chinese caterpillar fungus hypha early annesl enter the original hase idiophase, therefore go out grass early, shortened the cultivation cycle, main is favourable at process of growth formation higher effective composition.The drying of product is handled, and adopts the normal temperature baking, and temperature is no more than 50 ℃, has all kept original composition and biologically active basically.For the production that guarantees that northern Chinese caterpillar Fungus of the present invention is stable, in the aweto producing process of the present invention, strain cultivation preferably adopts liquid shaking table shaken cultivation liquid spawn, and particularly shaking frequency is 4660-4800 time/hour.Such strain cultivation has breeds fast, growth cycle weak point, and early provides prerequisite for going out grass (fruit body).The mycelial growth of preferably liquid spawn culture is thread finely dispersed bacterium liquid and is directly used in and produces cultivation, or place preserve under the 2-4 ℃ of condition standby.The medium of liquid spawn is selected the mixing filtrate of rice and carrot for use, and the liquid spawn of turning out is more suitable for inoculating the growth of cultivation.
The present invention compared with the prior art, it is as follows to have advantage: to the excellent species seed selection of " Cordyceps militaris ", a series of researchs such as the industrial and annual culture technique of the ecological condition of Artificial Control temperature, humidity, illumination, air and product low-temperature bake method, not only solved the technology of " Cordyceps militaris " a large amount of rapid artificial annually cultivatings, and output height, success rate height (going out carelessness reaches more than 90%), good product quality has kept tens kinds of active ingredients and bioactive stability in the cordyceps militaris sporocarp substantially.Wherein the main component content of cordycepin is up to 11mg/g, cordycepic acid 13.47%, Cordyceps sinensis polysaccharide 15.4%, SOD enzyme 386.36u/g total amino acid content 30514.59mg/100g, protein 37.42%, all be higher than wild cordyceps 3-5 doubly more than.
Specific embodiment:
Embodiment 1, a kind of northern Chinese caterpillar Fungus of the present invention are produced the planting technique flow process, earlier with preferred northern Chinese caterpillar Fungus bacterial strain, and carry out strain cultivation.Be used to produce the liquid spawn employing liquid shaking table shaken cultivation of cultivation, the concussion frequency is 4670 times/hour.
Medium: select the good northeast rice of meter matter for use, in 1: 1.3 ratio of material-water ratio, in the 770 gram vials of packing into, every bottled rice 50 grams.(water transfers to PH7.5-8) heating is cooled off standby 122 ± 1 ℃ of autoclavings 2 hours.
Liquid cultivation seed: under aseptic condition, liquid spawn evenly is spilled into media surface rapidly, inoculum concentration 5mL/ bottle (also available rice of medium and corn short, bristly hair or beard mixed culture medium can be received effect same).
Cultivating chamber is cultivated management: artificially controlling temperature, wet, light and conditions of air are created best ecotope.
1. adopt the posture stereo cultivation method.
2. send out bacterium initial stage natural daylight and cultivate, but can not be by the sunlight direct projection.Temperature is at 16-18 ℃, the growth that begins to sprout of 24 hours mycelia, the mycelia color is by the leucismus orange colour when being covered with media surface 1/2-2/3 in 3-4 days, and the needle-like fruit body primordium occurred in 8-13 days, begins to increase illumination, the oxygen therapy of abroaching, attention temperature, humidity, ventilating management simultaneously.Have every day must reach between the light time more than 9 hours, (illumination 200-300Lux) also can assist by fluorescent lamp.Whole sporophore growth phase temperature be controlled at 19 ± 3 ℃ the most desirable, relative air humidity 65-75%, day and night temperature difference 2-3 ℃, ventilation every day 0.5-1 hour.
3. from being inoculated into the 69 days whole production cycles of gathering,, must in time gathering, otherwise influence output and quality when stroma (careless strain) color has reached the ripe phase by the deep orange look thin out is gradually when yellow.Every bottle of average bright product 18-23 gram, dry product 2 gram-2.5. gram, biological transformation ratio average 50% of producing.
4. can continue after receiving to carry out second phase cultivation into new medium, so circulation can be produced 3-4 cycle in 1 year.
The product oven dry is preserved: for keeping active ingredient and the biologically active in the cordyceps militaris sporocarp, adopt the air drying technology.Can adopt electricity or fire bed formula baking box, fresh Chinese caterpillar fungus be placed on the screen cloth by thickness grading toast, 30 ℃ of final temperatures of initial temperature can not the baking above 50 ℃, and time 3-4 hour, water content was lower than 5%.Lovely luster, aromatic flavour.Packing into seals in plastic sack or the paper bag, puts low temperature drying under the 2-5 ℃ of condition, keeps in Dark Place, and the 2-3 nondiscolouring is never degenerated.
Embodiment 2, a kind of northern Chinese caterpillar Fungus of the present invention are produced cultivation technological method, carry out strain cultivation with preferred northern Chinese caterpillar Fungus bacterial strain earlier.Strain cultivation adopts the shaking table shaken cultivation, and the concussion frequency is 4780 times/hour.
Medium: select the corn short, bristly hair or beard for use, in 1: 1.7 ratio of material-water ratio, in the 770 gram vials of packing into, every bottled medium 50 grams.Water transfers to PH7.5-8, and heating was 128 ± 2 ℃ of autoclavings 2 hours.
Liquid cultivation seed: under aseptic condition, liquid spawn evenly is spilled into media surface, inoculum concentration 8mL/ bottle rapidly.(also available rice of medium and corn short, bristly hair or beard mixed culture medium can be received effect same.)
Cultivating chamber is cultivated:
1. adopt posture body cultivation, the bottle of inoculation makes full use of the space from falling down to be arranged on the upright shelf.
2. send out bacterium initial stage natural daylight and cultivate, but can not be by the sunlight direct projection.Temperature is at 18-20 ℃, the growth that begins to sprout of 24 hours mycelia, the mycelia color is by the leucismus orange colour when being covered with cultivation basal plane 1/2-2/3 in 3-4 days, pin shape entity original hase occurred in 15-17 days, begin to increase illumination, the oxygen therapy of abroaching, note temperature, humidity, ventilating management simultaneously, have every day must reach between the light time more than 9 hours, (illumination 200-300Lux) can assist by fluorescent lamp.Whole sporophore growth phase temperature be controlled at 18 ± 3 ℃ the most desirable, relative air humidity 60-75%, day and night temperature difference 2-3 ℃, ventilation every day 0.5-1 hour.
3. spray the 90 days whole production cycles of gathering that are seeded into from liquid spawn,, must in time gather, otherwise influence output and quality when stroma (careless strain) color has reached the ripe phase by the deep orange look thin out is gradually when yellow.Every bottle of average bright product 18-23 gram, dry product 2 gram-2.5. gram, biological transformation ratio average 50% of producing.
4. can continue to carry out the second phase into new medium after receiving and cultivate, so circulation can be produced 3-4 cycle in 1 year.
Bright product oven dry:, adopt the air drying technology for keeping active ingredient and the biologically active in the cordyceps militaris sporocarp.Can adopt electricity or fire bed formula baking box, fresh Chinese caterpillar fungus be placed on the screen cloth by thickness grading toast, 25 ℃ of final temperatures of initial temperature can not the baking above 50 ℃, time 3-4 hour, reaches water content and is lower than at 5% o'clock and stops baking.Lovely luster, aromatic flavour.Packing into seals in plastic sack or the paper bag, puts low humidity drying under the 2-5 ℃ of condition, keeps in Dark Place, and nondiscolouring in 3 years is never degenerated.
Embodiment 3, a kind of northern Chinese caterpillar Fungus of the present invention are produced cultivation technological method, and preferred northern Chinese caterpillar Fungus bacterial strain carries out strain cultivation.Liquid spawn is adopted the shaking table shaken cultivation, and the concussion frequency is 4790 times/hour.
Medium: select for use the northeast rice to mix at 1: 1 with the corn short, bristly hair or beard, in 1: 1.5 ratio of material-water ratio, in the 770 gram vials of packing into, every bottled medium 50 grams.Water transfers to PH7.5-8, and heating was 130 ± 5 ℃ of autoclavings 2.5 hours.
Liquid cultivation seed: under aseptic condition, liquid spawn evenly is spilled into media surface, inoculum concentration 5mL/ bottle rapidly.
Cultivating chamber is cultivated:
1. adopt the posture stereo cultivation method.
2. send out bacterium initial stage natural daylight and cultivate, but can not be by the sunlight direct projection.Temperature is at 16 ± 2 ℃, the growth that begins to sprout of 24 hours mycelia, the mycelia color is by the leucismus orange colour when being covered with cultivation basal plane 2/3 in 4 days, the needle-like fruit body primordium occurred in 8-13 days, begin to increase illumination, the oxygen therapy of abroaching, note temperature, humidity, ventilating management simultaneously, have every day must reach between the light time more than 9 hours, (illumination 200-300Lux) is auxiliary with fluorescent lamp.Whole sporophore growth phase temperature be controlled at 17 ± 2 ℃ the most desirable, relative air humidity 70-75%, day and night temperature difference 2-3 ℃, ventilation every day 0.5-1 hour.
3. from being inoculated into the 80 days whole production cycles of gathering,, must in time receiving, otherwise influence output and quality when stroma (careless strain) color has reached the ripe phase by the deep orange look thin out is gradually when yellow.Every bottle of average bright product 18-23 gram, dry product 2 gram-2.5. gram, biological transformation ratio average 50% of producing.
4. can continue into new medium after receiving, carry out the second phase and cultivate, so circulation can be produced 3 cycles in 1 year.
Bright product oven dry:, adopt the air drying technology for keeping active ingredient and the biologically active in the cordyceps militaris sporocarp.Can adopt electricity or fire bed formula baking box, fresh Chinese caterpillar fungus be placed on the screen cloth by thickness grading toast, 35 ℃ of final temperatures of initial temperature can not the baking above 50 ℃, 3.4 hours time, reaches water content and is lower than at 5% o'clock and stops baking.Lovely luster, aromatic flavour.Packing into seals in plastic sack or the paper bag, puts low temperature drying under the 2-5 ℃ of condition, keeps in Dark Place, and nondiscolouring in 2.5 years is never degenerated.
Claims (8)
1, a kind of aweto producing process, with preferred northern Chinese caterpillar Fungus bacterium, carry out strain cultivation, it is characterized in that: the A. medium is selected the good northeast rice of meter matter or corn short, bristly hair or beard or northeast rice and the mixing of corn short, bristly hair or beard for use, pack in the bottle in the ratio of material-water ratio 1: 1.2-1.7, cool off after the slaking through the sterilization of heating, under aseptic condition, liquid spawn is spilled into media surface after, with bottle closure; The bottle that B. will have medium and a bacterial classification is put into the natural daylight of not direct projection and cultivates down, and temperature 16-20 ℃, the mycelia growth that begins to sprout, after the mycelia color became orange colour and the appearance of needle-like fruit body primordium is arranged, the oxygen therapy of abroaching had to reach 9-10 hour between the light time every day; Be controlled at 17-22 ℃ in whole sporophore growth phase temperature, relative air humidity 65-75%; C. the fruit-body color in later stage of growing has reached the ripe phase by the deep orange look thin out is gradually when yellow, gathers, can not baking with 25-30 ℃ of initial temperature, maximum temperature above 50 ℃, and water content is lower than 5%.
2, as the said aweto producing process of claim 1, it is characterized in that: strain cultivation adopts liquid shaking table shaken cultivation liquid spawn, and frequency is 4660-4800 time/hour.
3, as claim 1 or 2 said aweto producing process, it is characterized in that: the mycelial growth of strain cultivation is thread finely dispersed liquid spawn and is directly used in the production cultivation.
4, as claim 1 or 2 said aweto producing process, it is characterized in that: the mycelial growth of strain cultivation is thread finely dispersed liquid spawn, place under the 2-4 ℃ of condition preserve standby.
5, as claim 1 or 2 said aweto producing process, it is characterized in that: the medium of liquid spawn is selected the mixing filtrate of rice and carrot for use, and the liquid spawn of turning out is more suitable for inoculating the growth of cultivation.
6, as the said aweto producing process of claim 3, it is characterized in that: the medium of liquid spawn is selected the mixing filtrate of rice and carrot for use, and the liquid spawn of turning out is more suitable for inoculating the growth of cultivation.
7, as the said aweto producing process of claim 4, it is characterized in that: the medium of liquid spawn is selected the mixing filtrate of rice and carrot for use, and the liquid spawn of turning out is more suitable for inoculating the growth of cultivation.
8, as claim 1 or 2 said aweto producing process, it is characterized in that: the medium water transfers to PH7.5-8.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100468008A CN100493335C (en) | 2005-06-28 | 2005-06-28 | Aweto producing process |
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| CNB2005100468008A CN100493335C (en) | 2005-06-28 | 2005-06-28 | Aweto producing process |
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| CN1887049A true CN1887049A (en) | 2007-01-03 |
| CN100493335C CN100493335C (en) | 2009-06-03 |
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| CNB2005100468008A Expired - Fee Related CN100493335C (en) | 2005-06-28 | 2005-06-28 | Aweto producing process |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101724562A (en) * | 2008-10-20 | 2010-06-09 | 大庆市帅亿生物质技术有限公司 | Method for producing cordceps militaris by off-tank liquid strain |
| CN101843196A (en) * | 2010-04-19 | 2010-09-29 | 杨毅 | Method for culturing mycelia and sporocarps of aweto |
| CN101897275A (en) * | 2010-08-13 | 2010-12-01 | 义乌市丹溪药用生物开发研究所 | Selenium-rich cordyceps culturing method |
| CN102017865B (en) * | 2009-09-23 | 2012-05-16 | 北京农业生物技术研究中心 | Two-stage large-scale cordyceps militaris culture method |
| CN102523927A (en) * | 2012-01-17 | 2012-07-04 | 辽宁仙榆湾北冬虫夏草（集团）有限公司 | Method for culturing cordyceps militaris in natural environment |
| CN102523908A (en) * | 2010-12-08 | 2012-07-04 | 解竣文 | Cordyceps militaris strain liquid amplification technology |
| CN102550290A (en) * | 2012-01-17 | 2012-07-11 | 辽宁仙榆湾北冬虫夏草（集团）有限公司 | Method for cultivating cordyceps militaris strain |
| CN102893809A (en) * | 2012-10-31 | 2013-01-30 | 长阳金梅生物科技有限公司 | Method for preparing cordyceps militaris liquid strain |
| CN103283480A (en) * | 2012-03-05 | 2013-09-11 | 何寒 | Method for growing cordyceps sinensis fruiting body directly in liquid medium |
| CN105638231A (en) * | 2014-11-07 | 2016-06-08 | 江苏学府生物工程有限公司 | Cordyceps militaris high-yield method |
| CN106718031A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris hot weather cultivating technique |
| CN106718036A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris moves cultivating technique |
| CN108464193A (en) * | 2018-02-09 | 2018-08-31 | 庄乙潇 | A kind of Cordceps militaris high yield cultivates lighting environment system and its control method |
-
2005
- 2005-06-28 CN CNB2005100468008A patent/CN100493335C/en not_active Expired - Fee Related
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101724562A (en) * | 2008-10-20 | 2010-06-09 | 大庆市帅亿生物质技术有限公司 | Method for producing cordceps militaris by off-tank liquid strain |
| CN102017865B (en) * | 2009-09-23 | 2012-05-16 | 北京农业生物技术研究中心 | Two-stage large-scale cordyceps militaris culture method |
| CN101843196A (en) * | 2010-04-19 | 2010-09-29 | 杨毅 | Method for culturing mycelia and sporocarps of aweto |
| CN101843196B (en) * | 2010-04-19 | 2012-07-04 | 杨毅 | Method for culturing mycelia and sporocarps of aweto |
| CN101897275A (en) * | 2010-08-13 | 2010-12-01 | 义乌市丹溪药用生物开发研究所 | Selenium-rich cordyceps culturing method |
| CN101897275B (en) * | 2010-08-13 | 2011-08-10 | 义乌市丹溪药用生物开发研究所 | Selenium-rich cordyceps culturing method |
| CN102523908A (en) * | 2010-12-08 | 2012-07-04 | 解竣文 | Cordyceps militaris strain liquid amplification technology |
| CN102550290A (en) * | 2012-01-17 | 2012-07-11 | 辽宁仙榆湾北冬虫夏草（集团）有限公司 | Method for cultivating cordyceps militaris strain |
| CN102523927A (en) * | 2012-01-17 | 2012-07-04 | 辽宁仙榆湾北冬虫夏草（集团）有限公司 | Method for culturing cordyceps militaris in natural environment |
| CN102550290B (en) * | 2012-01-17 | 2015-06-10 | 辽宁华夏三宝生物资源科技开发有限责任公司 | Method for cultivating cordyceps militaris strain |
| CN103283480A (en) * | 2012-03-05 | 2013-09-11 | 何寒 | Method for growing cordyceps sinensis fruiting body directly in liquid medium |
| CN103283480B (en) * | 2012-03-05 | 2014-11-05 | 何寒 | Method for growing cordyceps sinensis fruiting body directly in liquid medium |
| CN102893809A (en) * | 2012-10-31 | 2013-01-30 | 长阳金梅生物科技有限公司 | Method for preparing cordyceps militaris liquid strain |
| CN102893809B (en) * | 2012-10-31 | 2014-11-12 | 湖北仁仁生物科技有限公司 | Method for preparing cordyceps militaris liquid strain |
| CN105638231A (en) * | 2014-11-07 | 2016-06-08 | 江苏学府生物工程有限公司 | Cordyceps militaris high-yield method |
| CN106718031A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris hot weather cultivating technique |
| CN106718036A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris moves cultivating technique |
| CN108464193A (en) * | 2018-02-09 | 2018-08-31 | 庄乙潇 | A kind of Cordceps militaris high yield cultivates lighting environment system and its control method |
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|---|---|
| CN100493335C (en) | 2009-06-03 |
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