Background technology:
Edible medicinal fungi and human health have substantial connection, such as: tens kinds of fungies such as glossy ganoderma, white fungus, hedgehog hydnum, mushroom and Chinese caterpillar fungus. In October, 2014 has been retrieved, and result illustrates: Patent Office of the People's Republic of China receives the application for a patent for invention about Chinese caterpillar fungus just more than 1,000 items. Chinese caterpillar fungus is a class entomogenous fungi, parasitic in larva, on the earth about 400 kinds, and various Chinese caterpillar fungus selects different parasitized larvae. Cordyceps sinensis as medicinal material is parasitic in bat moth larvae the earliest, and the place of production is limited to High aititude, severe cold areas, i.e. the Qinghai of China, Tibet, Gansu and Sichuan. The fifties in last century, add Cuningham through further investigation, first find that Cordyceps militaris has higher pharmaceutical value. Cordyceps militaris is also known as Cordyceps militaris (L.) Link., northeast Cordyceps sinensis, Cordyceps militaris (L.) Link., pupa grass, parasitic in pupa body. From the nineties in 20th century, Cordyceps militaris (Cordyceps militaris (L.) Link.) artificial cultivation method obtains the biological transformation ratio of about 100% in production. Compare through document: see: 1, the cultivation raw material of the present invention and step are not identical. 2, the biological transformation ratio of the present invention is different from method in the past, reaches more than 200%, so, have different completely technically.
The invention has the beneficial effects as follows the protein content height of raw material, it is to increase edible fungus living being transformation efficiency; Particularly the ratio of moisture is different from front. Through checking document, it does not have with the method for overlap of the present invention. So, it is proposed to inventive method new below.
Summary of the invention:
The object of the present invention: the high-yield method cultivating Cordyceps militaris (Cordyceps militaris (L.) Link.) is provided.
Describe method and step below:
1. the method for a Cordyceps militaris high yield, the feature of the present invention is: taking wheat, rice as the substratum of main component, take high protein as additive, by sterilization, inoculation liquid Cordyceps militaris bacterial classification, add suitable moisture, do not need mixing, after loading container, carry out the method managed according to known cultivation technique, obtaining the biological transformation ratio of more than 200%, present method can industrialization, automatic production.
2. the feature of the present invention is: taking rice, wheat, corn, millet, glutinous millet, highland barley, supporting wheat, barley, sorghum rice grain constituents as substratum, the composition summation of grain class is at 90-95%.
3. the feature of the present invention is: taking protein, VITAMIN and inorganic salt as additive, and ratio is 5-10%, and the protein ratio wherein added is at 4.9-9.9%. According to how much protein can use soya-bean cake, wheat bran, peanut soya-bean cake, rapeseed cake, Semen Helianthi cake and milk powder, soya-bean milk, egg ingredients; Or the mixture of silkworm, silkworm chrysalis and tussah, cocoon chrysalis, it is important to employ high protein composition.
4. the feature of the present invention is: inoculation liquid Cordyceps militaris (Cordyceps militaris (L.) Link.) bacterial classification.
5. the part by weight of solid culture medium and liquid is 100: 220. Simultaneously, it is not necessary to mix, processing sequence is reduced. Usual way uses part by weight to be 100: 150-160. Method has basic difference.
6. the feature of the present invention is: involved Cordyceps militaris, with the kind (at the bacterial classification of bacterial classification center preservation before patent application day) of country's bacterial classification center preservation: Cordyceps militaris (Cordyceps militaris (L.) Link.), manufacture, with above bacterial classification, new technology, the novel method that liquid-spawn inoculation completes in grain class culture material.
The novel method that the technology of the present invention is produced has the following advantages: 1 and the biological cost compare of usual way, greatly reduces production cost; 2, biological transformation ratio being doubled, same raw material doubles above in output; 3, after substratum loads container, it is not necessary to mixing; 4, can cultivate on streamline, for reducing costs, carry out automatic production, open wide prospect; 5, realize Chinese caterpillar fungus and the whole-course quality control of effective constituent processing, establish reliable basis for Chinese materia medica product moves towards world market.
Embodiment:
Taking wheat, rice as the substratum of main component, taking 8% protein as additive, by sterilization, inoculation liquid Cordyceps militaris bacterial classification, adds suitable moisture, it is not necessary to mixing, every box 30 grams of solid culture based raw materials, after loading container, carry out the method managed according to known cultivation technique, on average obtain the biological transformation ratio of more than 200%.
Reference:
1, Wang Guodong chief editor: Cordyceps sinensis class ecology cultivates application, science and technology Literature publication society first version in August nineteen ninety-five 158,172
2, Li Hao: Chinese caterpillar fungus artificial cultivation technique Golden Shield press in November, 2000 first version 29.