200948371 九、發明說明: 【發明所屬之技術領域】 本發明係有關一種冬蟲夏草菌絲體及子實體的繁殖培養,能使 成品效能提昇的方法與液態培養基,尤指一種兼具中草藥成份轉 化調理質性的冬蟲夏草菌絲體及子實體之培養方法與液態培養 基。 【先前技術】 ❹ 冬蟲夏草分為Cordyceps sinensis (中華蟲草)、c〇rdyceps militaris (CM、北蟲草、蛹蟲草、)和其它種別,為同屬真菌的 模式種’是寄生於_鮮術名為鱗翅目柄㈣蟲蟲草编竭蛾 (H印ialus arm〇ricanus 0berthur)幼蟲上的子座,或其它種別 的編蝎科昆蟲幼蟲上的複合體;冬蟲夏草屬於g_生物,是珍 貴的傳統醫藥材料之…其主要營養及藥物成份如蟲草素、蟲草 多糖、蛋白質、氨基酸、維生素、礦物質等。蟲草在中國社會傳 峨念使肖它能增紐魏,通常作為關食物或献進被削弱 的性作用’具紐復性雜之醫藥轉代表。無論野生冬蟲夏草、 人工菌絲體、人工子實體及其活性發酵產物均已成為功能營養補 給品的重要資源。其化學成分組成包括有&草素(3,_去氧料 :)和它_生物、麥相醇、錢糖、包含錢基異丁酸的釀 2和肽;它們有抗腫瘤,反轉移,免疫調節,抗氧化,抗發炎 調〜:於心血管系統、呼吸系統和腎臟等疾病的 ^和雜。中華蟲草菌絲體麵性可以提高性欲以及生育能 力的相關研究,以及它的_機解影響,最年經過國内外 200948371 =各界之研究已_證㈣表絲。料 功效,如能益肝腎、防癌、抗癌、止血 j多種樂用 生產繁殖蟲草菌絲體或子實體的方法,係典^大f 接入嘛上输軸树== Γ異f2 子實體’細上蝴單方献,為使其效能 ❹ ^街上t如職方成份的溫和平衡_質性發揮,確有在方 Γ發明發的必要’雜料無果得佳肯定。 本發明係㈣長械製、研究過財所㈣的—種突 果,並已能從一項實際樣品試驗研究報告( = 到良好的成效證實。 一)中件 土 ^發明之主要目的係在提供一種冬蟲夏草菌絲體之培養方 法’係取人參、刺五加、蛇床子、紅景天、丁香、肉堇謹 萃r並分離其成份成為中獅縮 ' 培養液射草藥濃縮液齡調製成為巾草藥營養 液;由培養H皿錄R〇水添加人中草藥營養液,且經過渥熱滅 菌後冷卻處理成驗態培養基,再將蟲草g種活化接 養基上’透過震祕養方式,培養出蟲草菌絲體;藉由菌絲^ 長過程轉中草樂成份的轉化作用,俾菌絲體經洪乾研磨處理後 之粉劑成品’能兼具中草藥·_理質性,獲得品質及效能 提昇。 本發明之次-目的係在提供一種冬蟲夏草子實體之培養方 法’係取人參、刺五加、蛇床子、紅景天、T香、岐蔻、洋蔥 等中草藥植物精濃縮萃取,並分離其成份成為中草藥濃縮 200948371 液,先將P CA培養液與中草藥濃縮液混合調製成為中草藥營養 液,由培養器皿盛裝RO水添加入中草藥營養液,且經過溼熱滅 菌後冷卻處理成為液態培養基,並在培養器凰内置入穀物後,再 將蟲草菌種活化接種至液態培養基及穀物上,透過靜置培養方 > 式’培養出蟲草子實體;藉由子實體生長過程吸收中草藥成份的 „ 轉化作用,俾摘取子實體經烘乾研磨處理後之粉劑成品,能兼具 中草藥成份的調理質性,獲得品質及效能的提昇。 © 本發明之再-目的係在提供—種冬蟲夏草祕體及子實體之 液態培養基’係依重量比例’取人參5%、刺五加廳、蛇床子3〇%、 '红景天30%、丁香10%、肉蓋蔻跳、洋蔥热等中草藥植物,用酒 精20倍濃鮮取’並用低溫旋轉賴濃縮分離機,分離出主成份 成為中草藥濃縮液;且以1:1的混合比例,將p c A培養液與中 草藥漢縮液混合調製為中草藥營養液;由大型培養器皿容器盛裝 ⑽水’再將比例佔0.5%的中草藥營養液加入R〇水中,並經過 減菌爸以120 0125 C溫度溼熱滅菌丄5分鐘後,冷卻處理成為液200948371 IX. Description of the invention: [Technical field of the invention] The present invention relates to a method for breeding and cultivating the mycelium and fruiting bodies of Cordyceps sinensis, which can improve the performance of the finished product and the liquid medium, especially a combination of Chinese herbal medicine ingredients. Method for culturing the mycelium and fruiting bodies of Cordyceps sinensis and liquid medium. [Prior Art] 冬 Cordyceps sinensis is divided into Cordyceps sinensis (Chonghua Cordyceps), c〇rdyceps militaris (CM, Cordyceps militaris, Cordyceps militaris, and other species), which is a model species of the same genus. a complex of larvae on the larvae of the larvae of the genus (H), or other species of larvae of the genus Codidae; the worm is a g_bio, a precious traditional medical material. ...the main nutrients and pharmaceutical ingredients such as cordycepin, cordyceps polysaccharides, proteins, amino acids, vitamins, minerals, etc. Cordyceps sin in Chinese society, so that it can increase the Wei Wei, usually as a food or contribution to the weakened sexual role. Both wild Cordyceps sinensis, artificial mycelium, artificial fruit bodies and their active fermentation products have become important resources for functional nutritional supplements. Its chemical composition includes & oxalate (3, _ deoxidizer:) and its _ biological, wheat phase alcohol, money sugar, brewed 2 and peptide containing keto isobutyric acid; they have anti-tumor, anti-metastasis , immune regulation, anti-oxidation, anti-inflammatory regulation ~: in the cardiovascular system, respiratory system and kidney diseases and miscellaneous. The decent body of Chinese Cordyceps mycelium can improve the related research on sexual desire and fertility, as well as its influence on the machine. The most recent year at home and abroad 200948371 = the research of all circles has been _ certificate (four) silk. The efficacy of the material, such as the benefits of liver and kidney, anti-cancer, anti-cancer, hemostasis, a variety of methods for the production of Cordyceps mycelium or fruiting body, the system of the code ^ big f access to the axis tree == strange f2 fruiting body 'Small on the single side of the butterfly, in order to make it effective ❹ 街上 街上 街上 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 温 如 温The invention is based on (4) the long-term system and the research of the financial institution (4), and can be confirmed from an actual sample test report (= to good results. Providing a method for culturing a mycelium of Cordyceps sinensis, taking ginseng, acanthopanax, Cnidium, Rhodiola, clove, meat chop and extracting the ingredients into a medium lion's culture medium Towel herbal nutrient solution; adding human Chinese herbal nutrient solution by cultivating H dish, R-water, and then cooling it into a test medium after sterilizing by heat, and then activating the culture medium of Cordyceps sinensis The mycelium of the cordyceps; the transformation of the components of the grass by the process of the hyphae and the long process, the finished powder of the mycelium after the grinding and drying process can be combined with the Chinese herbal medicine _ physique, quality and efficacy Upgrade. The second objective of the present invention is to provide a method for cultivating a fruit body of Cordyceps sinensis, which is obtained by extracting and extracting Chinese herbal medicines such as ginseng, acanthopanax, Cnidium, Rhodiola, T, sputum, onion, etc., and separating the components thereof. Become a Chinese herbal medicine concentrate 200948371 solution, first mix the P CA culture solution with the Chinese herbal medicine concentrate to prepare a Chinese herbal nutrient solution, add the RO water into the Chinese herbal nutrient solution from the culture vessel, and after cooling and sterilizing, it is cooled to become a liquid medium, and in the incubator After the phoenix is built into the grain, the Cordyceps species are inactivated and inoculated into the liquid medium and the grain, and the Cordyceps sinensis is cultured through the static culture side; the Phytophthora component is absorbed by the growth process of the fruit body, and the ginseng is extracted. The powdered product obtained by drying and grinding the fruiting body can combine the conditioning property of the Chinese herbal medicine component, and obtain the improvement of quality and efficiency. © The present invention is to provide a liquid state of the Cordyceps sinensis secretarial body and the fruit body. The medium 'according to the weight ratio' takes ginseng 5%, acanthopanax table, Cnidium 3%, 'red sedum 30%, clove 1 0%, fleshy caps, onion heat and other Chinese herbal medicines, using alcohol 20 times thick and fresh' and using a low temperature rotary concentrating separator to separate the main components into a Chinese herbal medicine concentrate; and in a 1:1 mixing ratio, pc The A culture solution is mixed with the Chinese herbal medicine Han liquid to prepare a Chinese herbal nutrient solution; the large-scale culture vessel container (10) water' is added to the 0.5% Chinese herbal nutrient solution into the R water, and the heat-reducing dad is heated at 120 0125 C. After sterilizing for 5 minutes, the cooling treatment becomes liquid.
態培養基;據以蟲_種活化接魅液態培養基上,或在液態培 $基上再置人有穀物時’可培養出含中草誠份質性的蟲草菌絲 體或子實體。 【實施方式】 種冬蟲夏草菌絲體之培養方法,如圖1,餘人參、刺五 加、蛇床子、紅暑车 τι 用#香、肉莖蔻、洋蔥等中草藥植物10, ' I 取,並分離其成份成為中草藥濃縮液11 ;先將Ρ 200948371 ίί培與中草藥濃縮液11混合調製成為中草藥營養液 1 3,由培養器皿雜R〇^ 4添加人中草藥營養液丨3,且 後冷卻處理成為雜培養基15,再將蟲草菌種2 / 至液態培養基i 5上,透過震動培養方 培養__體16,·藉由菌絲體16生長過程吸收 卓樂成伤的轉化作用,俾菌絲體1 6經40t〜501溫度烘乾及i ❹ ❹ 二:,後之粉劑成品17,能兼具中草藥成份的調理質 性’獲得品質及效能的提昇。 =據上述第-實施例,本發明可由以下第二實施例培養出蟲 卓^其是-種冬蟲夏草子賴之轉方法,域2,係取 人參、刺五加、蛇床子、紅景天、丁香、肉蓋謹、洋葱等中草藥The culture medium of the Cordyceps sinensis or the fruiting body can be cultured on the basis of the insect-activated liquid medium or when the grain is re-set on the liquid. [Embodiment] The cultivation method of the Cordyceps sinensis mycelium, as shown in Fig. 1, the ginseng, the acanthopanax, the snake bed, the red summer car τι with the #香, meat stem 蔻, onion and other Chinese herbal plants 10, 'I take, and The ingredients are separated into a Chinese herbal medicine concentrate 11; firstly, Ρ200948371 ίί培 is mixed with the Chinese herbal medicine concentrate 11 to prepare a Chinese herbal nutrient solution 1 3, and the human herbal nutrient solution 丨3 is added from the culture vessel R〇^4, and then cooled to become The miscellaneous medium 15 is further applied to the liquid medium i 5 on the liquid medium i 5 , and the culture medium __ body 16 is shaken by shaking culture. The growth of the mycelium 16 is absorbed by the growth process of the mycelium 16 1 6 After 40t~501 temperature drying and i ❹ ❹ 2: After the powder finished product 17, can combine the conditioning quality of Chinese herbal ingredients to improve the quality and efficiency. According to the above-mentioned first embodiment, the present invention can be cultivated by the following second embodiment, which is a method for transferring the genus Cordyceps sinensis, domain 2, and taking ginseng, acanthopanax, Cnidium, Rhodiola, Chinese herbal medicines such as cloves, meat cover, and onions
植物1 〇 ’義精濃縮萃取,並分離其成份成為中草藥濃縮液i 1,先將PCA培養液12與中草藥濃驗U 草藥營養液13;由培養器皿盛裝扣水14添加人中草^= 液13 ’且經過渥熱滅菌後冷卻處理成為液態培養基工5,並在 培養器皿内置入穀物i 5A後,再將蟲草菌種2 〇活化接種至液 悲培養基1 5及榖物(如食用米)! 5 A上,透過靜置培養方式, 在2〇°C〜25°C環境下培養出蟲草子實體i 6 A ;藉由子實體丄6 a 生絲程吸收中草藥成份的轉化作用,俾摘取子實體丄6缝5〇 °C〜80°C溫度烘乾及! 2 〇目研磨處理後之粉劑成品i 7 a,能 具中草藥成份的調理,獲得品質及效能的提昇。 根據上述第-及第二實施例,本發明可由以下第三實施例得到 理想的液態培養基;其是-種冬蟲夏草菌絲體及子實體之液態培 200948371 養基’如圖3 ’係依重量比例,取人參5%、刺五加ι〇%、蛇床子 30%、紅景天30%、丁香10%、肉莖蔻10%、洋蔥5%等中草藥植物 1 0,用酒精20倍濃缩萃取,並用低溫旋轉減壓濃縮分離機分 離出主份成為中草藥濃縮液1 1 ;且以1:1的混合比例,將p c A培養液12與中草藥濃縮液11混合調製為中草藥營養液工 3 ;由大型培養器孤容器盛裝尺〇水14 (逆滲透乾淨水),再將 〇 比例佔0.5%的中草藥營養液1 3加入RO水1 4中,並經過滅菌 釜以120 〇125。(:溫度溼熱滅菌1 5分鐘後,冷卻處理成為液態培 養基15 ;如圖1、2,據以蟲草菌種2 〇活化接種至液態培養 . 基15上,或在液態培養基15上再置入有穀物i 5A時,可培 養出含中草藥成份質性的蟲草菌絲體16或子實體16A。 根據上述第一及第二實施例之菌種來源與取得方式,因屬習 知’在此僅作概要實施陳述;其中’如圖工、2 ’接種至液態培 ❹養基1 5上的活化菌種,可取自踊蟲草活之菌體,在肌〜饥溫 至中’採暗培養的方式,以培養孤作複製性菌種繁殖3〜5天, |達丨正常菌量後移至冰箱作8種保存;該g種保存期限最長一 個月,必須取出做菌種2 〇活化,且菌種2〇活化為丄〇天内接 菌至液態培養基1 5上使用·。 根據上述第一及第二實施例,其中’如圖1 0活化接種卿術W嶋培養基15 的重量比例約為1:10可得到良好的培養成效。 ❹Plant 1 〇 'Yi Jing concentrated extraction, and separate its ingredients into a Chinese herbal juice concentrate i 1, first PCA culture solution 12 and Chinese herbal medicine concentrated U herbal nutrient solution 13; from the culture vessel containing dehydration 14 added human grass ^ = liquid 13 'and after heat sterilization, it is cooled to become liquid culture medium 5, and after the grain i 5A is built into the culture vessel, the Cordyceps species 2 〇 is activated and inoculated to the liquid sorrow medium 1 5 and the sputum (such as edible rice). ! On 5 A, the Cordyceps fruiting body i 6 A was cultured in a static culture mode at 2 ° ° C ~ 25 ° C; the fruiting body was absorbed by the fruit body 丄 6 a silk process, and the fruit body was extracted.丄6 slit 5〇 °C ~ 80 °C temperature drying and! 2 After the grinding process, the powder finished product i 7 a can be adjusted with Chinese herbal ingredients to improve the quality and efficiency. According to the above-mentioned first and second embodiments, the present invention can obtain a desired liquid medium from the following third embodiment; it is a liquid culture of Cordyceps sinensis mycelium and fruiting bodies 200948371 Nutrient group as shown in Figure 3 Take ginseng 5%, acanthopanax ι〇%, Cnidium 30%, Rhodiola 30%, clove 10%, meat stem 蔻 10%, onion 5% and other Chinese herbal plants 10, concentrated with alcohol 20 times And using a low-temperature rotary vacuum concentration separator to separate the main portion into a Chinese herbal medicine concentrate 1 1; and mixing the pc A culture solution 12 with the Chinese herbal medicine concentrate 11 in a mixing ratio of 1:1 to prepare a Chinese herbal nutrient liquid 3; The large-scale incubator is filled with a squid water 14 (reverse osmosis clean water), and then the Chinese herbal nutrient solution 1 of 0.5% of the sputum is added to the RO water 14 and passed through a sterilizer at 120 〇 125. (: After the temperature is moist heat-sterilized for 15 minutes, it is cooled to become the liquid medium 15; as shown in Fig. 1, 2, the Cordyceps species 2 is activated and inoculated to the liquid culture. The base 15 or the liquid medium 15 is placed again. In the case of grain i 5A, Cordyceps mycelium 16 or fruit body 16A containing the constituent ingredients of Chinese herbal medicine can be cultured. According to the above-mentioned first and second embodiments, the source of the strain and the manner of obtaining it are known to be only known here. Summary implementation statement; in which the 'activated strains inoculated to the liquid culture medium on the 1st, 2' can be taken from the live cells of Cordyceps militaris, in the way of muscle ~ hunger to medium For the reproduction of the replicative strains for 3 to 5 days, the normal bacterial amount of the scorpion is transferred to the refrigerator for 8 kinds of preservation; the storage period of the g species is up to one month, and the strain must be removed and activated. The growth of the cockroach was carried out on a liquid medium for 15 days. In accordance with the first and second embodiments described above, the weight ratio of the activated sputum W嶋 medium 15 as shown in Fig. 10 was about 1: 10 can get good training results.
【主要元件符號說明】 中草藥植物1〇 P CA培養液12 RO 水 14 穀物1 5 A 子實體16A 菌種2 0 【附件說明】 200948371 根據上述各實施例所得到的蟲草菌絲體及子實體,本發明經 過一項「補充北蟲草增加SD大鼠之精液產量、活性、及激素調 控之研究」,如報告-、二,實驗結果證實,與未仙北蟲草菌絲 體與子實體混合物比較,補充食用北蟲草,能增加精液的質與量 之功效,如此,更加證明本發明方法與液態培養基,確能使培養 出來的冬蟲夏草菌絲體及子實體,具有良好品質及成效。 以上說明’謹屬本發明之較佳實施例,舉凡就上述實施例所作 之等效改變仍應屬本發明之方法範疇。 【圖式簡單說明】 圖1係本發明冬蟲夏草菌絲體培養方法流程圖 圖2係本發明冬蟲夏草子實體培養方法流程圖 •圖3係本發明冬蟲夏草液態培養基製作流程示意圖 中草藥濃縮液11 中草藥營養液13 液態培養基15 菌絲體1 6[Explanation of main component symbols] Chinese herbal medicine plant 1〇P CA culture liquid 12 RO water 14 grain 1 5 A fruiting body 16A strain 2 0 [Attachment description] 200948371 Cordyceps mycelium and fruit body obtained according to the above respective examples, The present invention has been subjected to a "study on the increase of semen production, activity, and hormonal regulation of SD rats by adding Cordyceps militaris," as reported in the report - and second, the experimental results confirmed that it is supplemented with the mixture of mycelium and fruiting bodies of Cordyceps militaris. The consumption of Cordyceps militaris can increase the quality and quantity of semen. Thus, it proves that the method and liquid medium of the present invention can ensure the quality and effectiveness of the cultured Cordyceps sinensis mycelium and fruit body. The above description is intended to be a preferred embodiment of the invention, and equivalent changes to the above-described embodiments are still within the scope of the method of the invention. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart of a method for culturing a Cordyceps sinensis mycelium according to the present invention. FIG. 2 is a flow chart of a method for cultivating a Cordyceps sinensis fruit body according to the present invention. FIG. 3 is a schematic view showing a process for preparing a liquid medium of Cordyceps sinensis according to the present invention. Chinese herbal medicine concentrate 11 Chinese herbal nutrient solution 13 Liquid medium 15 Mycelium 1 6
粉劑成品17、17A 附件一:本發明研究實驗英文報告 附件二:報告一之中譯本Powder finished products 17, 17A Annex I: English report of the research experiment of the invention Annex II: Report one Chinese translation