CN105831772A - Probiotics containing grape compound powder and preparation method thereof - Google Patents
Probiotics containing grape compound powder and preparation method thereof Download PDFInfo
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- CN105831772A CN105831772A CN201610203556.XA CN201610203556A CN105831772A CN 105831772 A CN105831772 A CN 105831772A CN 201610203556 A CN201610203556 A CN 201610203556A CN 105831772 A CN105831772 A CN 105831772A
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- powder
- vitis viniferae
- fructus vitis
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- fructus
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Abstract
The present invention discloses probiotics containing grape compound powder and a preparation method thereof. The grape powder with natural color and luster, flavor and taste are scientifically matched with functional products of lactobacillus plantarum powder, wheat malt powder, modified dietary fibers, enzyme powder, papaya powder, etc. The preparation method effectively improves the nutrition, food safety and health-care function of the grape compound powder and significantly improves the probiotic property and quality stability of the grape compound powder. Compared with the existing grape compound powder, the probiotics containing grape compound powder is simple in components, natural in color and luster, flavor and taste, high in nutritional value, strong in health-care function, high in contents of bioactive substances, high in number of viable probiotics, strong in probiotic properties, full in types and high in contents of probiotic functional metabolites, high in food safety, and long in shelf life.
Description
Technical field
The present invention relates to Fructus Vitis viniferae deep processed product, be specifically related to a kind of Fructus Vitis viniferae composite powder containing probiotic bacteria and preparation side thereof
Method.
Background technology
Solid beverage be moisture within 5%, there is definite shape, such as graininess, lamellar, bulk, powder
Deng, need to be through the dissolved beverage just can drunk.Solid beverage is removed moisture by liquid beverage and makes, and removes the mesh of moisture removal
: one is to prevent the enzyme of beverage itself or microbial deterioration or corruption, in order to storage；Two are easy for storing and transport.
Compared with liquid beverage, solid beverage has a characteristic that quality significantly alleviates, and volume significantly diminishes, easy to carry；Local flavor
Good, instant capacity is good, applied range, drinks conveniently；It is prone to keep health；Packaging is simple, convenient transportation.Solid beverage is with certain
Raw material is main, is equipped with what other adjuvant processed, by the classification of its primary raw material, solid beverage can be divided into Fruity type, egg
White type and three kinds of other type.They will add sugar or sweeting agent.Prior art often with some sweeting agent replace part or
All sucrose.However as increasing of auxotype disease, solid beverage, such as: solid milk tea, coffee creamer, bean milk powder, Semen Sesami
In paste, walnut powder etc., start to need to add composite sweetener low in calories with the problem solving energy and overnutrition.
Resveratrol is polyphenol compound, is mainly derived from the plants such as Fructus Vitis viniferae (red wine), Rhizoma Polygoni Cuspidati, Semen arachidis hypogaeae, Fructus Mori.
Resveratrol is a kind of the strongest biological natural polyphenol class material, is also called resvertrol, is the chemopreventive agent of tumor, is also
To reducing platelet aggregation, prevent and treat the chemopreventive agent of atherosclerosis, cardiovascular and cerebrovascular disease.United States Department of Agriculture (USDA)
Result of study shows, also contains considerable resveratrol in Testa arachidis hypogaeae and core.The experimentation of resveratrol it turned out
There is the beneficial effect to cardiovascular disease and cancer.Resveratrol has obvious preventive effect to hormone-dependent tumor.Also
Osteoporosis, acne and senile dementia can there be is preventive effect, there is antiviral and immunoregulation effect.To inside of human body one
Plant monomer defying age enzyme to work, and then play the latent effect preventing various age-related disease, extending life expectancy.White Herba chenopodii
Reed alcohol has health-care effect the most widely, such as Frenchman often to drink containing resveratrol in higher fatty acid thing, so French hat
Cardiopathia sickness rate is less than other western countries, just because of resveratrol is because high-fat preventive and therapeutic effect can be suppressed effectively,
Existing Countries and area develop resveratrol using resveratrol and goods thereof as dietary supplement.
At present with Fructus Vitis viniferae as raw material, prepare the less of pure grape fruit powder:
Chinese patent CN 102885265 A discloses the manufacture method of a kind of grape fruit powder.The present invention with fresh Fructus Vitis viniferae is
Raw material, after screening, heating carries out enzyme denaturing process, then carries out color fixative in zinc acetate solution and ascorbic acid solution, then
Drying, the Fructus Vitis viniferae after finally processing pulverizes and sieves, is vacuum-packed, sterilizing, obtains grape fruit powder finished product, and this product color is bright-coloured,
Nutritious, long shelf-life.The present invention has the advantages such as technique is simple and convenient to operate, production cost is low, is suitable for industry metaplasia
Produce.Patent color preservation technology tradition disclosed above, weak effect, introduce chemistry colour fixative, foodsafety is poor, uses heating enzyme denaturing
With drying, bioactive substance and heat-sensitive ingredients loss are big.
With Fructus Vitis viniferae as primary raw material, prepare the more of Fructus Vitis viniferae composite powder:
Chinese patent CN 104172005 A discloses a kind of prebiotics Fructus Vitis viniferae blue berry and is combined fruit powder and preparation method thereof, main
To be made up of following raw material: grape 50～80 parts, blueberry fresh fruit 20～50 parts, oligofructose 2～5 parts.Manufacture method is such as
Under: (1) takes grape and blueberry fresh fruit is cleaned；(2) crush and the edible sulfurous acid of addition obtains pulp in shattering process；(3)
Take the pulp of step (2), add pectase；(4) take the pulp after enzymolysis and squeeze to obtain blended fruit juice；(5) oligofructose is added；
(6) soluble solid content is concentrated into 25%；(7) filter；(8) lyophilization；(9) sieve to obtain fruit powder finished product.The present invention
Related products, rich in compositions such as anthocyanidin, organic acid, vitamin, have the highest nutritive value, quickly dissolving, can be used for drinking
The raw material of the food such as material, cake.Patent disclosed above uses traditional fruit juice preparation method nutrient component damages big, recovery rate
Low, health-care effect is undesirable.
Chinese patent CN 104970419 A discloses a kind of grape solids beverage, each including following weight/mass percentage composition
Individual component: Semen Vitis viniferae powder 20～25%, Fructus Pruni pseudocerasi dry powder 18～22%, blue berry dry powder 15～18%, Fructus psidii guajavae immaturus powder
15～17%, bacillus bifidus 3～5%, Protamax 4～6%, soy bean edible fiber powder 5～10%, vitamin 2～4%
With composite sweetener 2～5%.Grape solids beverage provided by the present invention has the advantage that the metabolism promoting body,
Strong body constitution；Cleaning intestinal rubbish, toxin expelling purify；Green natural fat reducing weight reducing etc..The composite sweetener added in beverage reduces
The disagreeable taste that Sucus Vitis viniferae solid drink itself is existing so that solid beverage sugariness is soft, is not result in dental caries, also will not draw
Play blood glucose cholesterol to raise.Patent functional materials disclosed above is Semen Vitis viniferae, and raw material availability is low, cost is high, food additive
Adding agent many, safety is low.
The the most more of fruit and vegerable composite powder is prepared for adjuvant with Fructus Vitis viniferae:
Chinese patent CN 102940214 B discloses a kind of vegetables and fruits fiber nutrition powder, described vegetables and fruits powder processing method
As follows: (1) pre-treatment: take fruit and vegetable materials, clean, remove the peel, pull an oar, it is thus achieved that fruit and vegerable serosity；(2) dried: take concentrated solution spray
Mist is dried, and obtains described fruit vegetable powder；(3) allotment: proportionally add；(4): packaging.Described fruit vegetable powder is by following weight fraction
Raw material compounding make: Plant fiber: 20 parts, plant colloid: 15 parts, probiotics factor: 10 parts, aminoacids complex 5 parts, ribose core
Acid: 0.6 part, Wheat Sprout Powder: 6 parts, ferment: 3 parts, malt extract: 10 parts, unsaturated fatty acid: 1 part, Guarana extract: 1 part, egg
White lead: 8 parts, flavoring agent: 0.03 part, compound vitamin and mineral: 0.6 part, 10 parts of vegetables and fruits powder.Vegetables and fruits powder taste of the present invention
Good, moisture is low easily stored, and high nutrition is low in calories, has good eliminating toxin and beautifying the skin effect, has lifting sleep matter simultaneously
Amount, weakens the effect of anxiety.Patent complicated components disclosed above, material treatment process is simple, and active substance loss is big,
Health-care effect is the most undesirable.
Chinese patent CN 104286756 A discloses a kind of fat-reducing fruit vegetable powder, having the natural plants of antiobesity action is
Raw material, uses low-temperature extraction and biological enzymolysis technology, remains effective ingredient therein to greatest extent, at existing fruit vegetable nutrient
On the basis of powder, science compounds dietary fiber, plant extract, Folium Camelliae sinensis extract, spice extract, Chinese herbal medicine extract, benefit
The fat-reducing effects such as raw bacteria powder, probiotics factor and bamboo charcoal powder are preferably lost weight the factor, have the most also compounded egg albumen powder, malt extract, little
Fructus Hordei Germinatus powder etc. have the nutrient substance of antiobesity action, and with the addition of the nutrients such as aminoacids complex, vitamin and mineral, mainly
From meeting the multi-angles such as satiety, regulation Biology Barrier of Gastrointestinal Tract, Traditional Chinese medicine health-preserving, liposuction row's fat, maintain and improve human body oneself
Regulate digestion and the level absorbed and ability, decrease picked-up and the secondary synthesis approach of body fat, just do not interfering with human body
Normal nutrient substance picked-up and metaboilic level, it is achieved the benign cycle of health preserving fat-reducing.Patent complicated components disclosed above, prebiotic
Bacterium uses tradition strain, and functional health effect is the most undesirable.
To sum up, with Fructus Vitis viniferae as primary raw material, a kind of mouthfeel of preparation and color and luster are natural, and nutritional labeling and bioactive substance contain
Amount height, component strong health-care effect simple, functional significantly contains the Fructus Vitis viniferae composite powder of probiotic bacteria and is still a kind of necessary.
Summary of the invention
Solved by the invention technical problem is that overcomes existing Fructus Vitis viniferae composite powder and the defect of preparation thereof, based on grape fruit powder
Want raw material, add probiotic powerful Lactobacillus plantarum powder, ferment powder, and science and compound Wheat Sprout Powder, modified dietary fiber etc.
A kind of mouthfeel of functional raw material preparation and color and luster are natural, and nutritional labeling and bioactive substance content are high, and component is simple, functional
By force, health-care effect significantly contains the Fructus Vitis viniferae composite powder of probiotic bacteria.
In order to achieve the above object, the present invention is by the following technical solutions:
A kind of Fructus Vitis viniferae composite powder containing probiotic bacteria, is mainly prepared by the raw material of following parts by weight:
Grape fruit powder 30-50 part, Wheat Sprout Powder 20-30 part, modified dietary fiber 8-15 part, papaya powder 6-12 part, plant breast
Bacillus powder 11-17 part, ferment powder 11-17 part, Fructus Citri Limoniae powder 5-10 part；
Preferably, the described Fructus Vitis viniferae composite powder containing probiotic bacteria, mainly prepared by the raw material of following parts by weight:
Grape fruit powder 35-45 part, Wheat Sprout Powder 23-27 part, modified dietary fiber 11-13 part, papaya powder 8-10 part, plant breast
Bacillus powder 13-15 part, ferment powder 13-15 part, Fructus Citri Limoniae powder 6-8 part；
It is highly preferred that the described Fructus Vitis viniferae composite powder containing probiotic bacteria, mainly prepared by the raw material of following parts by weight:
Grape fruit powder 40 parts, Wheat Sprout Powder 25 parts, modified dietary fiber 12 parts, papaya powder 9 parts, 14 parts of Lactobacillus plantarum powder,
Ferment powder 14 parts, Fructus Citri Limoniae powder 7 parts；
Further, described grape fruit powder is to protect through the instantaneous enzyme denaturing of microwave high-temperature, fixation and ultrasonic waves for cleaning, sericin peptide taken freezing
Protect, high-pressure pulse electric extraction, the extract at low temperature technology such as ultrasonic assistant microwave extraction, biological enzymolysis and prepare, to greatest extent
Remain the natural colored of Fructus Vitis viniferae, mouthfeel and bioactive ingredients thereof；
Preferably, the preparation method of described grape fruit powder, comprise the following steps, Fructus Vitis viniferae is put into and fills 0.1-0.3% carbonic acid
In the ultrasonic washing unit of hydrogen sodium solution, 200-400W, 35-45KHz clean 5-10min at room temperature, drain, each uva
Arbitrarily being cut into four parts, single flat is layered in microwave dryer in power 3-5kW, temperature 120-130 DEG C, is dried 3-5s, then
It is immersed in 8-10min in the sericin peptide taken solution that mass percent concentration is 8-12%, takes out, in-18--22 DEG C of freezing 30-
Pulverize immediately after 50min, ground product particle diameter 0.3-0.5mm, be subsequently added into the water of ground product quality 1-3 times, adjust with lactic acid
Joint pH value is 3.5-5.5, at room temperature in electric field intensity 25-35kV/cm, and burst length 300-500 μ s, pulse frequency 200-
High voltage pulse electric field processing 20-30min is carried out under the conditions of 300Hz；Then carry out micro-under the conditions of power 150-300W in room temperature
Amplitude, according to extracting 15-20min, simultaneously at power 200-300W, carries out ultrasonic assistant extraction under the conditions of frequency 30-40KHz；Add
Entering the mixed enzyme of extracting solution quality 1.5-2.5%, in 40-50 DEG C of enzymolysis 30-50min, enzymolysis solution filters, filtrate reduced in volume,
Lyophilization to moisture is that 5-8% i.e. obtains grape fruit powder；
Described mixed enzyme is that cellulase, protease, pectase, tannase 2-4:1-3:1-3:1-2 in mass ratio is uniform
Mixing.
Further, described Wheat Sprout Powder is with Semen Tritici aestivi as primary raw material, successively through ultrasonic cleaning, the instantaneous process of microwave,
The techniques such as ultrasonic wave added biological enzymolysis soaks, germinates, is dried, low-temperature grinding and prepare；
Preferably, the preparation method of described Wheat Sprout Powder, comprise the steps: to be placed on equipped with 0.01-0.03% Semen Tritici aestivi
In power 200-400W, frequency 20-30KHz room temperature ultrasonic cleaning 3-5min in the ultrasonic washing unit of sodium bicarbonate solution, take
Go out, drain, in frequency 2450MHz, power 3000W, temperature 38-42 DEG C, thickness of feed layer 2-4cm microwave drying 10-15s, then
Put temperature be 33-36 DEG C, pH value be 6-8 soak in soak 4-6h, it is 0.15-that soak contains mass percent concentration
The compound enzyme of 0.25%, ventilates once every 15-20min, ventilation pressure 0.13-0.15MPa, simultaneously in electric field intensity 10-
15kV/cm, burst length 100-300 μ s, pulse frequency 60-80Hz condition carries out high voltage pulse electric field processing, until Semen Tritici aestivi water
Dividing content is 40-45%；Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 20-24 DEG C,
The dark germination time is 20-24h, and wheat drying to the moisture after germinateing is 5-8%, and low-temperature grinding, excessively 60-80 mesh sieve are i.e.
Obtain Wheat Sprout Powder；
Described compound enzyme is that cellulase, 1,4 beta-glucanase, xylanase 2-4:2-4:1-3 in mass ratio uniformly mixes.
Further, described modified dietary fiber is that dietary fiber is processed through physics, chemical or biological method and obtained
To the soluble fiber cellulose content that there is strong retentiveness, dilatancy, thickening property, adsorptivity and network abundant high, biological
Activity is strong, have important, the cellulose of positive role, compared with full diet fiber, its biological activity to human body beneficial flora
Act on more powerful, also can be greatly prolonged the shelf-life of grape fruit powder；
Preferably, described modified fibre is by one or more in inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber
Obtain through enzyme enzymolysis；
It is highly preferred that the preparation method of described modified dietary fiber, comprise the following steps: by inulin, Semen Tritici aestivi fiber, Herba bromi japonici
Fiber 5-7:2-4:1-3 in mass ratio uniformly mixes, and adds the water of its quality 3-7 times, room temperature 100-300W, 35-40KHz condition
Supersound extraction 10-15min, then in electric field intensity 20-40kV/cm, burst length 300-500 μ s, pulse frequency 200-400Hz
Under the conditions of carry out high voltage pulse electric field processing 10-15min；It is 4.5-6.5 with breast acid for adjusting pH value, adds mixture quality 0.1-
The enzyme of 0.3%, in 45-55 DEG C of enzymolysis 20-48min；Enzymolysis solution filters, and filtrate reduced in volume, lyophilization contain to moisture
Amount i.e. obtains modified dietary fiber for 5-8%；
Described enzyme is that cellulase, xylanase, laccase, pectase 1-3:1-3:1-2:1-2 in mass ratio is uniform
Mixing.
Further, described Lactobacillus plantarum powder is with Lactobacillus plantarum CGMCC NO.11763 for starting strain routinely
Prepared by method, its viable bacteria content is: 7 × 1012-9×1012cfu/g；The cryoprotective agent that wherein freeze drying process uses
With the animal or plant containing antifreeze protein as raw material, extract through high-pressure pulse electric, ultrasonic assistant microwave extraction and compounding
Enzyme enzymolysis and prepared, Lactobacillus plantarum powder can be effectively improved at freezing dry process Viable detection；
Preferably, described protectant preparation method, comprise the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, sharkskin collagen egg
Being respectively washed in vain, drain, 8-10:3-5:2-4 in mass ratio uniformly mixes, and the pH value adding mixed material quality 0.1-1 times is
The lactic acid moistening 3-8h of 3.8-4.5, pulverizes after-18--22 DEG C of freezing 1-2h immediately, freezing thickness of feed layer 3-5cm, powder
Mince particle diameter 0.5-3mm, is subsequently added into the water of ground product quality 10-20 times, is 3.5-5.5 with breast acid for adjusting pH value, in room temperature
Under at electric field intensity 25-35kV/cm, burst length 300-500 μ s, carry out high-voltage pulse under the conditions of pulse frequency 200-300Hz
Electric field treatment 20-30min；Then under the conditions of power 150-300W, microwave irradiation and extraction 15-20min is carried out in room temperature, simultaneously
At power 200-300W, under the conditions of frequency 30-40KHz, carry out ultrasonic assistant extraction；Add the compounding of extracting solution quality 1-2%
Enzyme, in 45-55 DEG C of enzymolysis 30-50min；Enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that 0.1-0.3mm i.e. obtains guarantor
Protect agent；
Described compound enzyme is cellulase, protease, amylase, pectase, tannase 2-4:1-3:1-3 in mass ratio:
1-2:1-2 uniformly mixes；
Extract it is highly preferred that described microwave irradiation and extraction is batch (-type), i.e. microwave exposure 10s, be spaced 20s.
Further, described ferment powder preparation method is as follows: the fermentation liquid using pickle fermentation to produce discharge in latter stage is former
Material, the big grain material such as the dish leaf being filtered to remove in fermentation liquid obtains fermented liquid, and fermented liquid uses the dry sides such as lyophilization
Method prepares ferment powder.Cryodesiccated condition is :-30--40 degree pre-freeze 6 hours；Then lyophilizing is until water content is less than
5%.Pickle production method is prepared with reference to Chinese patent 201110421967.3 or 201210310308.7.
Another object of the present invention is to provide the preparation method of the above-mentioned Fructus Vitis viniferae composite powder containing probiotic bacteria, comprises the steps:
According to formula proportion, weigh each raw material, successively by ferment powder, Lactobacillus plantarum powder, grape fruit powder, Fructus Citri Limoniae powder, Wheat Sprout Powder, Fructus Chaenomelis
Powder adds V-type blending tank and uniformly mixes 25-35min, speed of agitator 20-40r/min, is subsequently adding modified dietary fiber and uniformly mixes
12-18min, speed of agitator 40-60r/min, sterile filling, seal, pack the Fructus Vitis viniferae composite powder i.e. obtained containing probiotic bacteria；
Preferably, described sterile filling uses automatic powdery filling machine fill, uses simultaneously60Co irradiation sterilization, irradiation agent
Amount 4kGy；
Preferably, described Fructus Vitis viniferae composite powder uses Aluminum-plastic composite bag fill；Ultraviolet is used before described Aluminum-plastic composite bag fill
Line or ozonization 20-40min.
The Fructus Vitis viniferae composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 6.84 × 1011-9.49×
1011cfu/g。
Lactobacillus plantarum of the present invention (Lactobacillus plantarum) XH is preserved in China on November 30th, 2015
CGMCC (is called for short) in Microbiological Culture Collection administration committee common micro-organisms center, and preserving number is CGMCC NO.11763, preservation
Address is: North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.
Lactobacillus plantarum probiotic properties is as follows:
Lactobacillus plantarum CGMCC NO.11763 provided by the present invention is found at pH be the condition of 1.50 through experiment
Lower survival, still in existing state after 1% cholate is cultivated 4 hours；Lactobacillus plantarum CGMCC NO.11763 degrades nitrous acid
Salt speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite is dense
Degree is at below 4.8mg/kg；CGMCC NO.11763, after fermentation 60h hour, can reach 64.76% to degrading rate of cholesterol.
CGMCC NO.11763 Adhering capacity measure from coagulation rate be 95.71%.
Lactobacillus plantarum CGMCC NO.11763 is to cholesterol degradation capability study and mensuration:
Take 1ml CGMCC NO.11763 mother solution and be inoculated in the MRS cholesterol fluid medium (cholesterol level of 10mL
0.1mg/ml, pH 6.2) in, it is standby, to access the MRS of 1mL sterilized water that the constant temperature standing of 37 DEG C cultivates 20h, 40h, 60h respectively
Cholesterol culture medium is comparison, takes at bacteria liquid sample and the comparison each 1ml of liquid, 9000r/min, 4 DEG C of above cultivation different time
Centrifugal 10min, obtains fermented supernatant fluid, and in o-phthalaldehyde method mensuration supernatant, cholesterol level is (particularly as follows: take each supernatant
0.1ml, in corresponding test tube, adds glacial acetic acid 0.3ml, and o-phthalaldehyde(OPA) 0.15ml of 1mg/ml is slowly added into concentrated sulphuric acid
1.0ml, mix homogeneously.Room temperature stands 10min, surveys light absorption value under 550nm).Each process 3 repetition, in kind makes
Make cholesterol standard curve, calculate cholesterol level and degradation rate in supernatant, the results are shown in Table 1.Understand, CGMCCNO.11763
Have good Degradation to cholesterol, after fermentation 60h hour, degradation rate can reach 64.76%.
The table 1 degraded situation to cholesterol.
| Degradation time (h) | 0 | 20h | 40h | 60h |
| Cholesterol level (mg/ml) | 0.2273±0.0058 | 0.1356±0.0018 | 0.1011±0.0094 | 0.801±0.0231 |
| Degrading rate of cholesterol % | 40.34% | 55.52% | 64.76% |
The bile tolerance test of Lactobacillus plantarum CGMCC NO.11763 bacterial strain:
Take CGMCCNO.11763 bacterium solution 1mL inoculation strain in containing different cholate (concentration gradients is 0.0%, 0.2%,
0.4%, 0.6%, 0.8%, 1%) 10mL MRS fluid medium (PH=6.4), be placed at 37 DEG C and cultivate 0 respectively, 2,4h,
Each process 3 repetition.Respectively take 1ml sample bacterium solution to mix in 9ml normal saline, prepare dilution factor solution, take 0.1ml dilution
Liquid is coated with in MRS, is inverted and cultivates 48 hours (each dilution factor do 3 parallel) record and calculate flat in 37 DEG C of biochemical cultivation cases
The several number of bacterium on plate.The results are shown in Table 2.The increment of bacterium is still after gallbladder salinity is 1% process 4h to understand this bacterium
Reach 0.59 ± 0.92 × 107(cfu/ml), there is good bile tolerance ability.
Table 2 bile tolerance ability detection [(± s) × 107cfu/ml]
The acid resistance test of Lactobacillus plantarum CGMCC NO.11763 bacterial strain
Take HLX37 mother solution by 1ml inoculation strain in different pH value (pH gradient is 1.5,2.0,2.5,3.0,3.5,4.0)
10mLMRS fluid medium, be placed at 37 DEG C and cultivate 0 respectively, 2,4h, each process 3 repetition.Respectively take 1ml sample bacterium solution in
9ml normal saline mixes, prepares dilute solution, take 0.1ml diluent and be coated with in MRS, fall in 37 DEG C of biochemical cultivation cases
Put the bacterium colony number cultivated on 48 hours (each dilution factor do 3 parallel) record flat board.The results are shown in Table 3.Illustrate that this bacterium has
The strongest acid-fast ability.
Table 3 acid-fast ability detection [(± s) × 107cfu/ml]
The Adhering capacity of Lactobacillus plantarum CGMCC NO.11763 measures
Cultivate CGMCC NO.11763 (MRS fluid medium), bacillus coli DH 5 alpha (LB fluid medium) 24h must ferment
Liquid, is respectively placed in 3000r/min, centrifugal 10min at 4 DEG C, collects bacterium mud, respectively with the sterile phosphate buffer of pH=7.0
(PBS) washing bacterium mud 2 times (i.e. adds PBS in bacterium colony, after concussion mix homogeneously, is placed in 3000r/min, centrifugal at 4 DEG C
10min, collects thalline).From coagulation rate (%): with aseptic PBS, bacterium mud CGMCC NO.11763 is formed at wavelength 600nm
The suspension bacteria liquid that light absorption value is 0.4 ± 0.1 (A 0) and bacteria suspension, stand and measure light absorption value A 24 after 24h, from coagulation rate
(%) formula is (A 0 A 24)/A 0.；His coagulation rate (%): by CGMCC NO.11763 and the outstanding bacterium of bacillus coli DH 5 alpha
Liquid is adjusted to the mix suspending bacterium solution that the light absorption value at wavelength 600nm is 0.6 ± 0.1 (A 0).Extinction is measured after standing 24H
Value A 24, his coagulation rate (%) formula is (A 0 A 24)/A 0.Measurement result is shown in Table 5, it is known that CGMCC NO.11763 from
Coagulation rate is 95.71%, has the strongest Adhering capacity.
Table 4 Adhering capacity table
The bacterial strain physiological property of Lactobacillus plantarum CGMCC NO.11763
It is micro-that described Lactobacillus plantarum (Lactobacillus plantarum) XH is preserved in China on November 30th, 2015
CGMCC (is called for short) in biological inoculum preservation administration committee's common micro-organisms center, and preserving number is CGMCC NO.11763, preservation ground
Location is: North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.
This bacterial strain feature is as follows: examine under a microscope, and this bacterial strain is rod-short, and Gram’s staining is positive, atrichia,
Do not produce spore；On solid medium, this bacterium bacterium colony is white, and smooth surface is fine and close, and form is circular, and edge is more neat.
Physicochemical characteristics is: catalase (-), gelatin liquefaction (-), indole experiment (+), mobility (-), fermentation gas
(-), nitrate reductase (-), fermentation gas (-), product hydrogen sulfide gas (-), pH4.0MRS culture medium grows (+).Pass through
Physiology and biochemistry is accredited as Lactobacillus plantarum (Lactobacillus plantarum), named Lactobacillus plantarum
(Lactobacillus plantarum)XH。
Bacterial strain can at 57 DEG C well-grown, glucose tolerance is 275g/L.
Lactobacillus plantarum of the present invention by gathering people Li Jianshu, isolated in Yoghourt from Xinjiang Uygur fellow-villager family,
Acquisition time on June 2nd, 2015.
Lactobacillus plantarum CGMCC NO.11763 of the present invention is found under conditions of pH is 1.50 survive through experiment,
Still in existing state after 1% cholate cultivation 4 hours；Lactobacillus plantarum CGMCC NO.11763 degrading nitrite speed is fast,
Capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at 4.8mg/
Below kg；CGMCCNO.11763, after fermentation 60h hour, can reach 64.76% to degrading rate of cholesterol.CGMCC
NO.11763 Adhering capacity measure from coagulation rate be 95.71%, bacterial strain can at 57 DEG C well-grown, glucose-tolerant energy
Power is 275g/L.
Beneficial effect:
The present invention Fructus Vitis viniferae composite powder containing probiotic bacteria will have grape fruit powder and the plant breast bar of natural colored, local flavor and mouthfeel
The functional product science such as mycopowder, Wheat Sprout Powder, modified dietary fiber, ferment powder, papaya powder compound, and are not only effectively increased Portugal
The trophism of grape composite powder, foodsafety and health care, and significantly improve the probiotic of Fructus Vitis viniferae composite powder and quality
Stability, compared with existing Fructus Vitis viniferae composite powder: its component is simple, and color and luster, local flavor and mouthfeel are natural, are of high nutritive value, keep healthy merit
Can be strong, bioactive substance content is high, and probiotics viable bacteria quantity is high, probiotic by force, and probiotic bacteria functional metabolic species is complete, contain
Amount height, foodsafety is high, long shelf-life.Concrete test effect is shown in embodiment seven-ten two, and concrete component effect is as follows:
1. the Lactobacillus plantarum CGMCC NO.11763 of the present invention is found under conditions of pH is 1.50 deposit through experiment
Live, still in existing state after 1% cholate is cultivated 4 hours；Lactobacillus plantarum CGMCC NO.11763 degrading nitrite speed
Degree is fast, and capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration exists
4.8mg/kg below；CGMCC NO.11763, after fermentation 60h hour, can reach 64.76% to degrading rate of cholesterol.CGMCC
NO.11763 Adhering capacity measure from coagulation rate be 95.71%.
2. the cryoprotective agent that the present invention uses when preparing Lactobacillus plantarum powder is by the winter higher containing antifreeze protein
Rye (Secale cereale L.), Caulis et Folium Ammopiptanthi Mongolici and sharkskin collagen protein science are compounding, through high-pressure pulse electric extraction, ultrasonic assistant microwave extraction and life
Thing enzymolysis and prepare, omnidistance extract at low temperature, antifreeze protein extraction ratio is high, and loss is few, the protective agent antifreeze peptide content obtained is high,
Kind is complete, functional by force, cryoprotective effects is good, improves the viable bacteria content in Lactobacillus plantarum powder.
3. the grape fruit powder that prepared by the present invention uses ultrasonic cleaning that Fructus Vitis viniferae carries out parasite killing to go out ovum, killing microorganisms, removal agriculture
Medicine residual and heavy metal ion etc., substantially increase the foodsafety of grape fruit powder；Use microwave high-temperature instantaneous dry, making
It is mainly enzyme denaturing, fixation while Fructus Vitis viniferae section explosion puffing drying, remains the natural colored of Fructus Vitis viniferae to greatest extent, solid through microwave
Fructus Vitis viniferae natural colored after color is highly stable in the follow-up course of processing, will not variable color, fade, color and luster is the most bright-coloured；Containing
There is in the aqueous solution of sericin peptide taken immersion rehydration, the loss of Fructus Vitis viniferae active substance that is chilled and that cause can be reduced to greatest extent,
Improve the extraction ratio of Fructus Vitis viniferae effective ingredient, also lay a good foundation at storage freezing-resistance for Fructus Vitis viniferae composite powder simultaneously；Will
The extract at low temperature technology such as high-pressure pulse electric, microwave, ultrasonic, biological enzymolysis organically combine, and can retain Fructus Vitis viniferae to greatest extent
Bioactive substance and flavor component.
4. the modified dietary fiber that prepared by the present invention is that dietary fiber is processed through physics, chemical or biological method and obtained
Arriving, soluble fiber cellulose content is high, biological activity is strong, have important, the cellulose of positive role to beneficial bacteria of intestinal tract group, with
Full diet fiber is compared, and its biological action is more powerful: 1) soluble fiber cellulose content is high, it is easier to by probiotic bacteria profit
With, probiotic bacteria can be improved in the growth of intestinal and fertility, increase kind and the quantity of probiotic bacteria flora, reduce large intestine pH
Value, improves intestine microenvironment；2) powerful absorbability, after modified, the specific surface area of cellulose increases, network
Abundant, absorption affinity strengthens, the organic molecule ability chelating, adsorbing cholesterol and bile acids is higher, suppression human body to they
Absorb；3) ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is higher；4) retentiveness, expansion
Property, thickening property is higher and acid and alkali, alkali, the impact of salt, 5) regulate and maintain the resident time of intestinal microbial population, strengthen disappearing of intestinal
Change and absorbability, improve body immunity, finally significantly improve digestion and the absorbance of Fructus Vitis viniferae composite powder effective ingredient；6) promote
Enter gastrointestinal peristalsis, slow down and eliminate the untoward reaction such as flatulence, abdominal distention；7) embedding effect is strong, can effectively prevent external environment (oxygen
Gas, temperature, illumination, humidity etc.) factor impact on product quality, stabilize the biological activity of product, extend the guarantor of product
The matter phase.
The preparation method of Wheat Sprout Powder the most of the present invention the Semen Tritici aestivi of immersion process is carried out ultrasonic cleaning, the instantaneous process of microwave and
High voltage pulse electric field processing effectively prevent soak pollution microbes, it is to avoid generation stink penetrates in Wheat Sprout Powder product,
Meanwhile, can effectively strengthen corn seed coat cell wall and the relative permeability of cell membrane, improve the water absorption rate of full cereal seed, promote kind
Son is sprouted in advance, improves generation speed and adenosine triphosphate (ATP) content of ultra-oxygen anion free radical, and stratification early stage is little
The activation of the multiple enzyme of Wheat Seeds and release, endosperm dissolve and functional nutrient composition, bioactive ingredients, antioxidant content
Synthesis, promote the respiratory metabolism of seed, accelerate nutrition and functional materials, bioactive ingredients, antioxygen chemical conversion
Point enrichment process, shorten enrichment time, improve nutrition and functional materials, bioactive ingredients, antioxidant content
Content, organically combines with biological enzymolysis, can degrade further seed coat cellulose and hemicellulose structure, increases seed coat permeability,
Activating various bioactive substance (endogenous enzymes etc.) vigor the strongest, enriching quantity is bigger, and soluble fiber cellulose content increases therewith, for
Lactobacillus plantarum in Fructus Vitis viniferae composite powder provides trophic factors, better significantly；By ultrasonic, microwave, high-pressure pulse electric
Technology and biological enzymolysis organically combine, and shorten soak time, improve germination percentage and germination uniformity；Can keep to greatest extent
Wheat Sprout Powder heat-sensitive substance content, especially antioxidant (glutathion, inositol hexaphosphate, vitamin C, polyphenol etc.),
Ensure the natural colored of Wheat Sprout Powder, taste and flavor to greatest extent, may also function as bactericidal action simultaneously；Particularly through microwave
After instantaneous appropriateness kills embryo, in the case of the biological activity not affecting Fructus Tritici aestivi seed, along with the absorption of moisture, wheat embryo
Being suppressed but do not affect every enzyme and the growth of bioactive substance and the dissolving of endosperm, blastogenesis length is the shortest, and Repiration is weak, sends out
Bud loss is low, improves yield and the quality of Wheat Sprout Powder, significantly improves functional in Wheat Sprout Powder product, trophism, life
The content of active substances, improve Wheat Sprout Powder health care (improve body immunity, remove oxygen-derived free radicals in human body,
Blood fat reducing, slow down aging), nutritive value and foodsafety.
6. the ferment powder that prepared by the present invention does not contain only acetobacter, bacillus bifidus, yeast and lactobacillus rhamnosus etc.
Purebred probiotic bacteria, and containing more, more useful participate in during pickle fermentation and the multiple wild microbial bacteria that produces
Strain, its biological activity and probiotic more comprehensively and powerful, the Lactobacillus plantarum powder science prepared with the present invention compounds, its effect
More significantly.
The preparation method technique of Fructus Vitis viniferae composite powder the most of the present invention is simple, easy to operate, low for equipment requirements, can industrialization and
Large-scale production, finally mixes modified dietary fiber, has played its powerful embedding effect so that the character of product is more steady
Calmly, the shelf-life is longer.
It should be noted that what the present invention contained the Fructus Vitis viniferae composite powder of probiotic bacteria has the technical effect that each component technical characteristic and side
The result that method feature is mutually collaborative, interact, the superposition of not simple technical characteristic (component function), each component technology is special
The combination levied and the collaborative effect produced, considerably beyond each monotechnics feature functionality and the superposition of effect, have preferably
Advance and practicality.
Detailed description of the invention
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention
It is method known in those skilled in the art.It addition, embodiment is interpreted as illustrative, and the unrestricted present invention
Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this
On the premise of invention spirit and scope, the various changes carrying out the material component in these embodiments and consumption or change are also
Belong to protection scope of the present invention.
Embodiment one:
Prepared by raw material
1, the preparation of Wheat Sprout Powder
Its preparation method comprises the steps:
Semen Tritici aestivi is placed in the ultrasonic washing unit equipped with 0.02% sodium bicarbonate solution in power 300W, frequency 25KHz
Room temperature ultrasonic cleaning 4min, takes out, drains, and does in frequency 2450MHz, power 3000W, temperature 40 DEG C, thickness of feed layer 3cm microwave
Dry 13s, then put temperature be 35 DEG C, pH value be 7 soak in soak 5h, soak contains mass percent concentration and is
The compound enzyme of 0.20%, ventilates once every 18min, ventilation pressure 0.14MPa, simultaneously in electric field intensity 13kV/cm, during pulse
Between 200 μ s, pulse frequency 70Hz condition carries out high voltage pulse electric field processing, until wheat water content content is 43%；After immersion
Semen Tritici aestivi drains, and carries out dark germination in growing floor, and dark germination temperature keeps 22 DEG C, and the dark germination time is 22h, little after germinateing
It is 6.5% that wheat is dried to moisture, and low-temperature grinding, 70 mesh sieves excessively i.e. obtain Wheat Sprout Powder；
Above-mentioned compound enzyme is that cellulase, 1,4 beta-glucanase, xylanase 3:3:2 in mass ratio uniformly mixes.
2, the preparation of modified dietary fiber
Its preparation method comprises the following steps:
Inulin, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 6:3:2 in mass ratio is uniformly mixed, adds the water of its quality 5 times, room temperature
200W, 38KHz condition supersound extraction 13min, then in electric field intensity 30kV/cm, burst length 400 μ s, pulse frequency 300Hz
Under the conditions of carry out high voltage pulse electric field processing 13min；It is 5.5 with breast acid for adjusting pH value, adds the biology of mixture quality 0.2%
Enzyme, in 50 DEG C of enzymolysis 34min；Enzymolysis solution filter, filtrate reduced in volume, lyophilization to moisture be 6.5% i.e. obtain modified
Dietary fiber；
Above-mentioned enzyme is that cellulase, xylanase, laccase, pectase 2:2:1.5:1.5 in mass ratio uniformly mixes.
3, the preparation of Lactobacillus plantarum powder
Lactobacillus plantarum powder is prepared with Lactobacillus plantarum CGMCC NO.11763 according to a conventional method for starting strain, its
Viable bacteria content is: 8 × 1012cfu/g；The cryoprotective agent that wherein freeze drying process uses with the animal containing antifreeze protein or
Plant is raw material, prepared through high-pressure pulse electric extraction, ultrasonic assistant microwave extraction and compound enzyme enzymolysis, can effectively carry
High Lactobacillus plantarum powder is at freezing dry process Viable detection；
Above-mentioned protectant preparation method comprises the steps:
Winter rye, Caulis et Folium Ammopiptanthi Mongolici, sharkskin collagen protein are respectively washed, are drained, and 9:4:3 in mass ratio uniformly mixes, and adds
Enter the lactic acid moistening 5h that pH value is 4.1 of mixed material quality 0.5 times, pulverize immediately after-20 DEG C of freezing 1.5h, freezing
Thickness of feed layer 4cm, ground product particle diameter 2mm, it is subsequently added into the water of ground product quality 15 times, is 4.5 with breast acid for adjusting pH value, in room
In electric field intensity 30kV/cm under temperature, burst length 400 μ s, carry out high voltage pulse electric field processing under the conditions of pulse frequency 250Hz
25min；Then under the conditions of power 225W, carry out microwave irradiation and extraction 18min, its microwave exposure 10s in room temperature, be spaced 20s,
Simultaneously at power 250W, under the conditions of frequency 35KHz, carry out ultrasonic assistant extraction；Add the compound enzyme of extracting solution quality 1.5%,
In 50 DEG C of enzymolysis 40min；Enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that 0.2mm i.e. obtains protective agent；
Above-mentioned compound enzyme is cellulase, protease, amylase, pectase, tannase 3:2:2:1.5:1.5 in mass ratio
Uniformly mixing；
4, the preparation of ferment powder
Its preparation method is as follows:
The big grain things such as the fermentation liquid using pickle fermentation to produce discharge in latter stage is raw material, the dish leaf being filtered to remove in fermentation liquid
Matter obtains fermented liquid, and fermented liquid uses the drying meanss such as lyophilization to prepare ferment powder.Cryodesiccated condition
It is :-35 degree pre-freezes 6 hours；Then lyophilizing is until water content is 3%.Pickle production method is with reference to Chinese patent
201110421967.3 preparation.
Wheat Sprout Powder that following example two to six are used, modified dietary fiber, Lactobacillus plantarum powder, ferment powder by
Prepared by embodiment one.
Embodiment two:
A kind of Fructus Vitis viniferae composite powder containing probiotic bacteria, is mainly prepared by the raw material of following parts by weight:
Grape fruit powder 40 parts, Wheat Sprout Powder 25 parts, modified dietary fiber 12 parts, papaya powder 9 parts, 14 parts of Lactobacillus plantarum powder,
Ferment powder 14 parts, Fructus Citri Limoniae powder 7 parts；
The wherein preparation method of grape fruit powder, comprises the following steps:
Being put into by Fructus Vitis viniferae in the ultrasonic washing unit filling 0.2% sodium bicarbonate solution, 300W, 30KHz are clear at room temperature
Washing 8min, drain, each uva is arbitrarily cut into four parts, and single flat is layered in microwave dryer in power 4kW, temperature 125
DEG C, be dried 4s, be then immersed in 9min in the sericin peptide taken solution that mass percent concentration is 10%, take out, in-20 DEG C of freezings
Pulverize immediately after 40min, ground product particle diameter 0.4mm, be subsequently added into the water of ground product quality 2 times, with breast acid for adjusting pH value
It is 4.5, at room temperature in electric field intensity 30kV/cm, burst length 400 μ s, carry out high-voltage pulse under the conditions of pulse frequency 250Hz
Electric field treatment 25min；Then under the conditions of power 225W, microwave irradiation and extraction 18min is carried out in room temperature, simultaneously at power 250W,
Ultrasonic assistant extraction is carried out under the conditions of frequency 35KHz；Add the mixed enzyme of extracting solution quality 2.0%, in 45 DEG C of enzymolysis
40min, enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are 6.5% i.e. to obtain grape fruit powder；
Above-mentioned mixed enzyme is that cellulase, protease, pectase, tannase 3:2:2:1.5 in mass ratio uniformly mixes.
The preparation method of the Fructus Vitis viniferae composite powder containing probiotic bacteria, comprises the steps:
According to formula proportion, weigh each raw material, successively by ferment powder, Lactobacillus plantarum powder, grape fruit powder, Fructus Citri Limoniae powder, Semen Tritici aestivi
Bud powder, papaya powder add V-type blending tank and uniformly mix 30min, speed of agitator 30r/min, are subsequently adding modified dietary fiber equal
Even mixed 15min, speed of agitator 50r/min, sterile filling, seal, pack the Fructus Vitis viniferae composite powder i.e. obtained containing probiotic bacteria；
Above-mentioned sterile filling uses automatic powdery filling machine fill, uses simultaneously60Co irradiation sterilization, irradiation dose 4kGy；
Above-mentioned Fructus Vitis viniferae composite powder uses Aluminum-plastic composite bag fill；Disinfection by ultraviolet light is used before described Aluminum-plastic composite bag fill
30min。
The Fructus Vitis viniferae composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 9.49 × 1011cfu/g。
Embodiment three:
A kind of Fructus Vitis viniferae composite powder containing probiotic bacteria, is mainly prepared by the raw material of following parts by weight:
Grape fruit powder 35 parts, Wheat Sprout Powder 23 parts, modified dietary fiber 11 parts, papaya powder 8 parts, 13 parts of Lactobacillus plantarum powder,
Ferment powder 13 parts, Fructus Citri Limoniae powder 6 parts；
The wherein preparation method of grape fruit powder, comprises the following steps:
Being put into by Fructus Vitis viniferae in the ultrasonic washing unit filling 0.1% sodium bicarbonate solution, 200W, 35KHz are clear at room temperature
Washing 5min, drain, each uva is arbitrarily cut into four parts, and single flat is layered in microwave dryer in power 3kW, temperature 120
DEG C, be dried 3s, be then immersed in 8min in the sericin peptide taken solution that mass percent concentration is 8%, take out, in-18 DEG C of freezings
Pulverize immediately after 30min, ground product particle diameter 0.3mm, be subsequently added into the water of ground product quality 1 times, with breast acid for adjusting pH value
It is 3.5, at room temperature in electric field intensity 25kV/cm, burst length 300 μ s, carry out high-voltage pulse under the conditions of pulse frequency 200Hz
Electric field treatment 20min；Then under the conditions of power 150W, microwave irradiation and extraction 15min is carried out in room temperature, simultaneously at power 200W,
Ultrasonic assistant extraction is carried out under the conditions of frequency 30KHz；Add the mixed enzyme of extracting solution quality 1.5%, in 40 DEG C of enzymolysis
30min, enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are 5% i.e. to obtain grape fruit powder；
Above-mentioned mixed enzyme is that cellulase, protease, pectase, tannase 2:1:1:1 in mass ratio uniformly mixes.
The preparation method of the Fructus Vitis viniferae composite powder containing probiotic bacteria, comprises the steps:
According to formula proportion, weigh each raw material, successively by ferment powder, Lactobacillus plantarum powder, grape fruit powder, Fructus Citri Limoniae powder, Semen Tritici aestivi
Bud powder, papaya powder add V-type blending tank and uniformly mix 25min, speed of agitator 20r/min, are subsequently adding modified dietary fiber equal
Even mixed 12min, speed of agitator 40r/min, sterile filling, seal, pack the Fructus Vitis viniferae composite powder i.e. obtained containing probiotic bacteria；
Above-mentioned sterile filling uses automatic powdery filling machine fill, uses simultaneously60Co irradiation sterilization, irradiation dose 4kGy；
Above-mentioned Fructus Vitis viniferae composite powder uses Aluminum-plastic composite bag fill；Disinfection by ultraviolet light is used before described Aluminum-plastic composite bag fill
20min。
The Fructus Vitis viniferae composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 8.13 × 1011cfu/g。
Embodiment four:
A kind of Fructus Vitis viniferae composite powder containing probiotic bacteria, is mainly prepared by the raw material of following parts by weight:
Grape fruit powder 45 parts, Wheat Sprout Powder 27 parts, modified dietary fiber 13 parts, papaya powder 10 parts, 15 parts of Lactobacillus plantarum powder,
Ferment powder 15 parts, Fructus Citri Limoniae powder 8 parts；
The wherein preparation method of grape fruit powder, comprises the following steps:
Being put into by Fructus Vitis viniferae in the ultrasonic washing unit filling 0.3% sodium bicarbonate solution, 400W, 45KHz are clear at room temperature
Washing 10min, drain, each uva is arbitrarily cut into four parts, and single flat is layered in microwave dryer in power 5kW, temperature 130
DEG C, be dried 5s, be then immersed in 10min in the sericin peptide taken solution that mass percent concentration is 12%, take out, in-22 DEG C of freezings
Pulverize immediately after 50min, ground product particle diameter 0.5mm, be subsequently added into the water of ground product quality 3 times, with breast acid for adjusting pH value
It is 5.5, at room temperature in electric field intensity 35kV/cm, burst length 500 μ s, carry out high-voltage pulse under the conditions of pulse frequency 300Hz
Electric field treatment 30min；Then under the conditions of power 300W, microwave irradiation and extraction 20min is carried out in room temperature, simultaneously at power 300W,
Ultrasonic assistant extraction is carried out under the conditions of frequency 40KHz；Add the mixed enzyme of extracting solution quality 2.5%, in 50 DEG C of enzymolysis
50min, enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are 8% i.e. to obtain grape fruit powder；
Above-mentioned mixed enzyme is that cellulase, protease, pectase, tannase 4:3:3:2 in mass ratio uniformly mixes.
The preparation method of the Fructus Vitis viniferae composite powder containing probiotic bacteria, comprises the steps:
According to formula proportion, weigh each raw material, successively by ferment powder, Lactobacillus plantarum powder, grape fruit powder, Fructus Citri Limoniae powder, Semen Tritici aestivi
Bud powder, papaya powder add V-type blending tank and uniformly mix 35min, speed of agitator 40r/min, are subsequently adding modified dietary fiber equal
Even mixed 18min, speed of agitator 60r/min, sterile filling, seal, pack the Fructus Vitis viniferae composite powder i.e. obtained containing probiotic bacteria；
Above-mentioned sterile filling uses automatic powdery filling machine fill, uses simultaneously60Co irradiation sterilization, irradiation dose 4kGy；
Above-mentioned Fructus Vitis viniferae composite powder uses Aluminum-plastic composite bag fill；Disinfection by ultraviolet light is used before described Aluminum-plastic composite bag fill
40min。
The Fructus Vitis viniferae composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 6.84 × 1011cfu/g。
Embodiment five:
A kind of Fructus Vitis viniferae composite powder containing probiotic bacteria, is mainly prepared by the raw material of following parts by weight:
Grape fruit powder 30 parts, Wheat Sprout Powder 20 parts, modified dietary fiber 8 parts, papaya powder 6 parts, 11 parts of Lactobacillus plantarum powder, ferment
11 parts of powder of element, Fructus Citri Limoniae powder 5 parts；
The wherein preparation method of grape fruit powder, comprises the following steps:
Being put into by Fructus Vitis viniferae in the ultrasonic washing unit filling 0.1% sodium bicarbonate solution, 400W, 35KHz are clear at room temperature
Washing 10min, drain, each uva is arbitrarily cut into four parts, and single flat is layered in microwave dryer in power 3kW, temperature 130
DEG C, be dried 3s, be then immersed in 8min in the sericin peptide taken solution that mass percent concentration is 12%, take out, in-22 DEG C of freezings
Pulverize immediately after 30min, ground product particle diameter 0.5mm, be subsequently added into the water of ground product quality 1 times, with breast acid for adjusting pH value
It is 5.5, at room temperature in electric field intensity 25kV/cm, burst length 500 μ s, carry out high-voltage pulse under the conditions of pulse frequency 200Hz
Electric field treatment 30min；Then under the conditions of power 150W, microwave irradiation and extraction 20min is carried out in room temperature, simultaneously at power 200W,
Ultrasonic assistant extraction is carried out under the conditions of frequency 40KHz；Add the mixed enzyme of extracting solution quality 1.5%, in 50 DEG C of enzymolysis
30min, enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are 8% i.e. to obtain grape fruit powder；
Above-mentioned mixed enzyme is that cellulase, protease, pectase, tannase 2:3:1:2 in mass ratio uniformly mixes.
The preparation method of the Fructus Vitis viniferae composite powder containing probiotic bacteria, comprises the steps:
According to formula proportion, weigh each raw material, successively by ferment powder, Lactobacillus plantarum powder, grape fruit powder, Fructus Citri Limoniae powder, Semen Tritici aestivi
Bud powder, papaya powder add V-type blending tank and uniformly mix 28min, speed of agitator 25r/min, are subsequently adding modified dietary fiber equal
Even mixed 13min, speed of agitator 45r/min, sterile filling, seal, pack the Fructus Vitis viniferae composite powder i.e. obtained containing probiotic bacteria；
Above-mentioned sterile filling uses automatic powdery filling machine fill, uses simultaneously60Co irradiation sterilization, irradiation dose 4kGy；
Above-mentioned Fructus Vitis viniferae composite powder uses Aluminum-plastic composite bag fill；Ozonization is used before described Aluminum-plastic composite bag fill
20min。
The Fructus Vitis viniferae composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 7.26 × 1011cfu/g。
Embodiment six:
A kind of Fructus Vitis viniferae composite powder containing probiotic bacteria, is mainly prepared by the raw material of following parts by weight:
Grape fruit powder 50 parts, Wheat Sprout Powder 30 parts, modified dietary fiber 15 parts, papaya powder 12 parts, 17 parts of Lactobacillus plantarum powder,
Ferment powder 17 parts, Fructus Citri Limoniae powder 10 parts；
The wherein preparation method of grape fruit powder, comprises the following steps:
Being put into by Fructus Vitis viniferae in the ultrasonic washing unit filling 0.3% sodium bicarbonate solution, 200W, 45KHz are clear at room temperature
Washing 5min, drain, each uva is arbitrarily cut into four parts, and single flat is layered in microwave dryer in power 5kW, temperature 120
DEG C, be dried 5s, be then immersed in 10min in the sericin peptide taken solution that mass percent concentration is 8%, take out, in-18 DEG C of freezings
Pulverize immediately after 50min, ground product particle diameter 0.3mm, be subsequently added into the water of ground product quality 3 times, with breast acid for adjusting pH value
It is 3.5, at room temperature in electric field intensity 35kV/cm, burst length 300 μ s, carry out high-voltage pulse under the conditions of pulse frequency 300Hz
Electric field treatment 20min；Then under the conditions of power 300W, microwave irradiation and extraction 15min is carried out in room temperature, simultaneously at power 300W,
Ultrasonic assistant extraction is carried out under the conditions of frequency 30KHz；Add the mixed enzyme of extracting solution quality 2.5%, in 40 DEG C of enzymolysis
50min, enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are 5% i.e. to obtain grape fruit powder；
Above-mentioned mixed enzyme is that cellulase, protease, pectase, tannase 4:1:3:1 in mass ratio uniformly mixes.
The preparation method of the Fructus Vitis viniferae composite powder containing probiotic bacteria, comprises the steps:
According to formula proportion, weigh each raw material, successively by ferment powder, Lactobacillus plantarum powder, grape fruit powder, Fructus Citri Limoniae powder, Semen Tritici aestivi
Bud powder, papaya powder add V-type blending tank and uniformly mix 33min, speed of agitator 35r/min, are subsequently adding modified dietary fiber equal
Even mixed 16min, speed of agitator 55r/min, sterile filling, seal, pack the Fructus Vitis viniferae composite powder i.e. obtained containing probiotic bacteria；
Above-mentioned sterile filling uses automatic powdery filling machine fill, uses simultaneously60Co irradiation sterilization, irradiation dose 4kGy；
Above-mentioned Fructus Vitis viniferae composite powder uses Aluminum-plastic composite bag fill；Ozonization is used before described Aluminum-plastic composite bag fill
40min。
The Fructus Vitis viniferae composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 6.99 × 1011cfu/g。
The change of T-CHOL after the edible Fructus Vitis viniferae composite powder containing probiotic bacteria of experimental example seven
Selecting the adult 48 of T-CHOL 180mg/dl-250mg/dl, men and women half and half, is randomly divided into three groups；First group
150 milliliters of mineral waters are drunk in dinner every day；Second group of dinner every day Instant Drinks common commercial glucose composite powder 150g, the 3rd group of every day
The Fructus Vitis viniferae composite powder 150g of the dinner Instant Drinks embodiment of the present invention 2, eats same food every day period, and food includes meat, egg, vegetables
Dish and fruit.Gather the blood of experimenter respectively in the previous day and the 20th, 40,60 days that experiment starts, measure in blood is total
Cholesterol level, result such as table 1:
Table 1: total cholesterol level testing result in blood
| Time | 0 day | 20 days | 40 days | 60 days |
| First group (mg/dl) | 202.3 | 203.8 | 206.2 | 209.3 |
| Second group (mg/dl) | 207.6 | 202.5 | 200.8 | 198.6 |
| 3rd group (mg/dl) | 208.8 | 200.9 | 189.5 | 167.3 |
As seen from the above table after the Fructus Vitis viniferae composite powder of the Instant Drinks embodiment of the present invention 2, containing of the T-CHOL in adult's blood
There is significant change in amount.Compared with common commercial glucose composite powder, Fructus Vitis viniferae composite powder of the present invention is to the total gallbladder in adult's blood
The content of sterin significantly reduces, and the content of the T-CHOL in mineral water composition year human blood significantly increases, commercially available Portugal
Although grape composite powder decreases, but compared with product of the present invention, effect is not notable, it follows that the present invention uses has fall
Fructus Vitis viniferae composite powder prepared by the specific bacterial strain of low cholesterol characteristic has the health-care effect well reducing cholesterol.
It should be understood that the Fructus Vitis viniferae composite powder containing probiotic bacteria prepared by embodiment of the present invention 3-6 has above-mentioned equally
Technique effect, between each embodiment, diversity is the most notable.
Embodiment eight present invention Fructus Vitis viniferae composite powder shelf-life implants living preparation of lactobacillus stable content examination containing probiotic bacteria
Test
Take the embodiment of the present invention 2 preparation Fructus Vitis viniferae composite powder store respectively under room temperature 22-25 DEG C, ventilation condition 3 months,
6 months, 12 months, 24 months, 36 months and measure Lactobacillus plantarum viable bacteria content, result such as table 2:
Table 2: shelf-life implants living preparation of lactobacillus content detection result
Result above shows: the storage stability of Fructus Vitis viniferae composite powder of the present invention is preferable, environment resistant (temperature, appropriateness, oxygen,
Illumination, moisture etc.) influence factor's ability is big, and shelf-life Lactobacillus plantarum viable bacteria content loss rate maximum (36 months) is 15%,
Higher than existing like product viable bacteria content, loss rate is low, long shelf-life, reflect that other of Fructus Vitis viniferae composite powder is biological simultaneously and live
Property composition has same storage stability.
It should be understood that the Fructus Vitis viniferae composite powder containing probiotic bacteria prepared by embodiment of the present invention 3-6 has above-mentioned reality equally
Test effect, between each embodiment and little with above-mentioned experiment effect diversity.
The sensory evaluation test of embodiment nine present invention Fructus Vitis viniferae composite powder containing probiotic bacteria
Invite Fructus Vitis viniferae composite powder and commercially available two kinds of similar identical dates of manufacture that the embodiment of the present invention 2 prepared by 24 personnel
Fructus Vitis viniferae composite powder judge, sense organ is given a mark, wherein specialty and each 12 of layman, and professional is young, the middle age, old
In each 4 of year, men and women half and half, and layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half；Marking includes outward appearance (20
Point), quality (25 points), local flavor (30 points), four aspects of mouthfeel (25 points), marking personnel independently carry out, are independent of each other, with ensure
Judge result accurate.Being added up judging result, equal score value takes approximation, retains integer, is specifically shown in Table 3:
Table 3: sensory evaluation statistical result
Note: show significant difference (P ＜ 0.05) with a line internal standard difference lowercase alphabet, the different capitalization of mark represents difference
Extremely notable (P ＜ 0.01), indicates same letter and represents that difference is not notable (P ＞ 0.05).
Result above shows, Fructus Vitis viniferae composite powder prepared by the present invention from outward appearance, quality, local flavor and mouthfeel any one all
Commercial glucose composite powder to be substantially better than, particularly outward appearance, local flavor and mouthfeel are fabulous, also are adapted for different age group, difference simultaneously
The consumer of hierarchy of consumption eats.
It should be understood that the Fructus Vitis viniferae composite powder containing probiotic bacteria prepared by embodiment of the present invention 3-6 has above-mentioned reality equally
Test effect, between each embodiment and little with above-mentioned experiment effect diversity.
The embodiment ten present invention probiotic test of Fructus Vitis viniferae composite powder containing probiotic bacteria
The Fructus Vitis viniferae composite powder embodiment of the present invention 2 prepared, being prepared as viable count with sterilized water is 2 × 1010CFU/mL's
Lactobacillus plantarum solution, saves backup in 4 DEG C；
1, the 10mL Lactobacillus plantarum solution kept of going bail for is injected in test tube 1, uses ten times of stepwise dilutions to 10-8, take
The MRS agar culture medium being cooled to 45 DEG C after sterilizing, on flat board, is poured on flat board (sterilizing), shakes rapidly by 1mL diluent
Even.The test tube 2 that will be equipped with 10mL Lactobacillus plantarum solution again is placed in 80-90 DEG C of water-bath heating 15-25min, after taking heating
Lactobacillus plantarum solution carry out ten times of stepwise dilutions to 10-8, take 1mL diluent on flat board, 45 DEG C will be cooled to after sterilizing
MRS agar culture medium pour into upper at flat board (sterilizing) and shake up rapidly.Finally by the flat board before heating and after heating all 35
Cultivate 24h under the conditions of DEG C, calculate the quantity before and after heating.
Result shows, Viable detection has reached more than 88%.
2, simulated gastric fluid and the resistance test of intestinal juice: the hydrochloric acid 16.4mL taking 100g/L adds distilled water diluting, makes pH value divide
It is not 1.5,2.5 and 3.5, takes 100mL dilute hydrochloric acid solution, be separately added into 1g pepsin so that it is fully dissolve, obtain simulation stomach
Liquid, microporous filter membrane degerming (0.22 μm) is standby.Taking potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, uses 0.1moL/L hydroxide
Sodium solution regulation pH value is to 6.8；Separately taking trypsin 10g, the 100mL that adds water makes dissolving, after two liquid mixing, is diluted with water to
1000ml, obtains simulated intestinal fluid, and microporous filter membrane degerming (0.22 μm) is standby.Take the Lactobacillus plantarum solution that 1mL keeps to join
In the simulated gastric fluid of 9mL (i.e. ten times stepwise dilutions), and the most fully mix rapidly, be subsequently placed in 30-45 DEG C of standing
Cultivate 2-4h.1h, 2h, 3h, 4h when, take out culture fluid respectively and count remaining viable count immediately, carrying out with former viable count
Relatively, result shows, Viable detection is 98%.Then each 1mL of culture fluid digesting different time it is taken in simulated gastric fluid, point
Be not inoculated in the simulated intestinal fluid that 9mL pH value is 6.8, be placed in 30-45 DEG C of quiescent culture 2-4h, and respectively 0,3,6,24h takes
Sample, measures its viable count, compares with former viable count, and result shows that Viable detection is 99%.
3, the resistance test of cholate is simulated: make the solution of 1g/L with pancreatin, and add the Fel Sus domestica of 0.8% in the solution
Salt, it is 8.0 that the NaOH with 10% adjusts pH, then with 0.45 μm micro-filtrate membrane filtration degerming.The plant breast that 0.5mL is kept
Bacillus solution inoculum is simulated in cholate to 4.5mL, obtains culture fluid, the viable count of counting remaining after cultivating 24h.Culture fluid is existed
In sterile saline, ten times of stepwise dilutions are to 10-8, and pour into MRS, it is subsequently placed in 35 DEG C of quiescent culture 24h.Result shows
Viable detection is 99%.
Above result of the test shows, Lactobacillus plantarum in Fructus Vitis viniferae composite powder of the present invention probiotic (thermostability, resistance to pH,
Bile tolerance) relatively strong, it is especially suitable for human intestines and stomach's environment, in simulated gastrointestinal environments, survival rate is big, can be effectively improved gastrointestinal function.
It should be understood that the Fructus Vitis viniferae composite powder containing probiotic bacteria prepared by embodiment of the present invention 3-6 has above-mentioned reality equally
Test effect, between each embodiment and little with above-mentioned experiment effect diversity.
Embodiment 11 present invention Fructus Vitis viniferae composite powder mouse intestinal performance test containing probiotic bacteria
The Fructus Vitis viniferae composite powder embodiment of the present invention 2 prepared, being prepared as viable count with sterilized water is 2 × 1010CFU/mL's
Lactobacillus plantarum solution, saves backup in 4 DEG C；
Choosing common Kunming white mice 60, male and female half and half, 18-20g, conventional word is supported.Therefrom random choose 40, every day
9:00 gavage lincomycin hydrochloride 0.2mL in morning (20mg)/only, other as a control group, every day same time gavage equivalent is gone out
Bacterium normal saline, continuous one week, the mouse model of preparation alteration of intestinal flora.Model group mouse diet declines, and death does not occurs
Significantly suffering from diarrhoea phenomenon, arrange soft excrement, profile normal aqueous divides more, and bedding and padding are moist.By 40 alteration of intestinal flora mices, with
Machine is divided into 2 groups, and one group 20 is only used as treatment group, the Lactobacillus plantarum solution 0.5ml (2 × 10 that every day, gavage was kept10cfu/
Ml)/only, another 20 are only used as natural recovering group, every day same time gavage equivalent sterile saline, continuous two weeks.Whole examination
Test the phase 21 days, observe growth and the defecation situation of white mice every day, in the 8th, 21 days to Fructus Vitis viniferae composite powder treatment group and the most extensive
The mice of multiple group is weighed, and calculates each group of weight average rate of increase, result such as table 4；Within every 5 days, survey each group of stool in mice large intestine bar
Bacterium quantity, calculates average, result such as table 5.Take stool in mice about 0.1g, add in aseptic operating platform 3 beades (with
0.1g excrement sample adds 0.5mL diluent), dilute and inoculate maconkey agar culture medium, calculate every gram wet just in coliform count.
Table 4: mice Gain weight
| Packet | Average starting weight (g/ is only) | Average end weight (g/ is only) | Average rate of increase (%) |
| Natural recovering group | 20.69±1.33 | 27.34±1.59 | 32.14a |
| Treatment group | 20.41±1.45 | 34.28±1.62 | 67.96b |
Table 5: the situation of coliform count in stool in mice
Fructus Vitis viniferae composite powder treatment group Mouse Weight average rate of increase (67.96%) is significantly higher than natural recovering group
(32.14%)；Feed solution rear intestinal escherichia coli quantity to be remarkably decreased, reduce by 97.07%, substantially less than natural recovering group
(24.78%), show the rapid field planting in white mice intestinal of the Lactobacillus plantarum in Fructus Vitis viniferae composite powder of the present invention, form dominant bacteria
Group, and effectively suppress the growth and breeding of the pathogen such as escherichia coli, and resident time is long, continues, effectively improves intestinal
Energy.
It should be understood that the Fructus Vitis viniferae composite powder containing probiotic bacteria prepared by embodiment of the present invention 3-6 has above-mentioned reality equally
Test effect, between each embodiment and little with above-mentioned experiment effect diversity.
The impact on immunity of organisms of embodiment 12 present invention Fructus Vitis viniferae composite powder containing probiotic bacteria
1 experiment purpose
By exercise tolerance test (mouse forced swimming), verify Fructus Vitis viniferae composite powder of the present invention raising immunity, resist tired
Work is used.
2 experiment materials and reagent
2.1 for reagent thing:
Commercial glucose composite powder (G1)；Commercial glucose composite powder (G2)；Fructus Vitis viniferae composite powder prepared by embodiment of the present invention 2-6
(G3-G7)。
2.2 reagent:
Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing；Concentrated sulphuric acid (AR), Nanjing chemical reagent has
Limit company；Normal saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..
3. laboratory animal
ICR mice, ♂, cleaning grade, body weight 18-22g, Ningxia Medical University's comparative medicine center provide, little during experiment
The free diet of Mus.
4. key instrument
Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof is public
Department；Water-bath: DK-S26 type, upper Nereid grand experimental facilities company limited；Electronic scale: BS224S type, Sartorius company；Second
Table, thermometer
5. experiment packet
5.1 dosage packets and given the test agent give the time and at random mice are divided into 8 groups, often group 10, the 1st group to the 7th group
Giving the medicine of G1～G7 respectively, the 8th group is blank group, gives isopyknic distilled water, and often the every average daily gavage of group 1 time, fills
Stomach volume is 0.2ml/10g, gives given the test agent continuously 30 days.
5.2 sample preparations the 1st group are to the 7th group: weigh 2.25g drug sample, be assigned to 150ml with distilled water；Blank
Group: distilled water 150ml.
6. experimental technique
6.1 swimmings with a load attached to the body experiment lasts are administered after 30min, put mice in swimming trunk, and the depth of water is no less than 30cm, water temperature 25
± 1 DEG C, the sheet lead of rat-tail root load 5% body weight, record mice swimming starts to the dead time, when swimming as mice
Between.
After 6.2 mice serum carbamide measure last administration 30min, not swimming with a load attached to the body 90min in the water that temperature is 30 DEG C,
Plucking eyeball blood sampling 0.5mL (being not added with anticoagulant) after rest 60min, put 4 DEG C of refrigerator 3h, after hemopexis, 2000r/min is centrifuged
15min, takes serum and send clinical laboratory of Affiliated Hospital of Ningxia Medical University to detect.
After the mensuration last of 6.3 hepatic glycogen is administered 30min, not swimming with a load attached to the body 90min in the water that temperature is 25 ± 1 DEG C,
Cervical dislocation puts to death mice, cleans with normal saline, and with after filter paper suck dry moisture, accurately weighs liver 100mg, and hepatic glycogen is examined
Test agent box detection Mouse Liver glycogen content.
The mensuration last of 6.4 blood lactase acid is taken a blood sample after being administered 30min, does not the most bear a heavy burden at the water went swimming that temperature is 30 DEG C
Stop after 10min.Lactic acid instrument assay method: respectively before swimming, each blood sampling 20 μ L add after rest 20min after swimming, after swimming
Entering in 40 μ L rupture of membranes liquid, the most fully vibration smudge cells lactic acid instrument measures.(blood lactase acid area under curve=5 × (before swimming
The blood lactase acid value of 20min after blood lactase acid value+2 × swimming of 0min after+3 × swimming of blood lactase acid value)
7. observation index walking weight load, blood lactase acid, carbamide, glycogen initial value
8. statistical method experimental data is usedRepresent, use t inspection to compare between organizing
9. experimental result
The impact on Mouse Weight of the 9.1 Fructus Vitis viniferae composite powders of the present invention
Each group mice after giving G1～G9 medicine, front, in, shown in post-weight see table respectively, at the beginning of each group mice
Initial body weight and weightening finish body weight compare equal no difference of science of statistics (P ＞ 0.05) with matched group, show that G1～G9 medicine is all without significantly
Toxicity.Experimental result refers to table 6.
The original body mass of table 6 swimming with a load attached to the body experiment mice, body weight in mid-term and end body weight
The impact on the mice burden swimming time of the 9.2 Fructus Vitis viniferae composite powders of the present invention
After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, can be obviously prolonged mice
Walking weight load, has significant difference (P ＜ 0.05), Fructus Vitis viniferae composite powder G3 of the present invention～G7 medicine and blank group ratio
Relatively, can significantly extend the mice burden swimming time, there is pole significant difference (P ＜ 0.01), and be substantially better than G1～G2 medicine
Thing.The results detailed in Table 7.
The impact on the mice burden swimming time of the table 7 Fructus Vitis viniferae composite powder
" * " p < 0.05vs blank；
" * * " p < 0.01vs blank；
9.3 Fructus Vitis viniferae composite powders of the present invention are on the impact of blood lactase acid before and after mouse movement
After per os gives the Fructus Vitis viniferae composite powder of the mice present invention, Fructus Vitis viniferae composite powder G3 of the present invention～G7 medicine are to mouse movement
Rear blood lactase acid area under curve compares with matched group significant difference (P ＜ 0.05), G1～G2 medicine group Mouse Blood lactic acid is bent
Though area compares with matched group and decreases under line, but and no difference of science of statistics (P ＞ 0.05).The results are shown in Table 8.
Table 8 Fructus Vitis viniferae of the present invention composite powder is on the impact of blood lactase acid level before and after mouse movement
" * " p < 0.05vs blank；
The impact on Mouse Liver glycogen of the 9.4 Fructus Vitis viniferae composite powders of the present invention
After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, and Mouse Liver glycogen content is equal
Have significantly rising, have significant difference (P ＜ 0.05), Fructus Vitis viniferae composite powder G3 of the present invention～G7 medicine with blank group
Relatively, Mouse Liver glycogen content all has significantly rising, has pole significant difference (P ＜ 0.01), and is substantially better than G1～G2
Medicine.The results detailed in Table 9.
The impact on Mouse Liver glycogen content of the table 9 Fructus Vitis viniferae of the present invention composite powder
" * " p < 0.05vs blank；
" * * " p < 0.01vs blank；
The impact on mice serum carbamide of the 9.5 Fructus Vitis viniferae composite powders of the present invention
After per os gives mice G1～G7 medicine, G1～G2 medicine group compares with blank group, serum after mouse movement
Urea content all has significantly reduction, have significant difference (P ＜ 0.05), Fructus Vitis viniferae composite powder G3 of the present invention～G7 medicine with
Blank group compares, and after mouse movement, serum urea content all has significantly reduction, has pole significant difference (P ＜
, and be substantially better than G1～G2 medicine 0.01).The results detailed in Table 10.
The impact on mice serum urea content of the table 10 Fructus Vitis viniferae of the present invention composite powder
" * " p < 0.05vs blank；
" * * " p < 0.01vs blank；
10. experiment conclusion
This experiment is mainly tested by mice burden swimming, and the deposit of detection Mouse Liver glycogen is observed the present invention and contained simultaneously
The Fructus Vitis viniferae composite powder of probiotic bacteria improves immunity, the effect of resisting fatigue.Preliminary Results shows as follows:
1, G3～G7 Fructus Vitis viniferae composite powder of the present invention all can extend the mice burden swimming time (P ＜ 0.01), and effect is obvious
It is better than the Fructus Vitis viniferae composite powder of other G1～G2.
2, biochemistry detection aspect shows, G3～G7 Fructus Vitis viniferae composite powder of the present invention each dosage group all can reduce Mouse Blood after motion
In clear, lactic acid content produced by glucose anerobic glycolysis, compares with matched group and has significant difference (P ＜ 0.05), and other G1
～although the Fructus Vitis viniferae composite powder of G2 also can reduce after motion lactic acid content produced by glucose anerobic glycolysis in mice serum, but
Compare with matched group, no difference of science of statistics (P ＞ 0.05)；
3, G3～G7 Fructus Vitis viniferae composite powder of the present invention each dosage group all can significantly improve deposit (the P ＜ of glycogen in mouse liver
, and effect is substantially better than the Fructus Vitis viniferae composite powder of other G1～G2 0.01)；
4, metabolic arthritis model finds, G3～G7 Fructus Vitis viniferae composite powder of the present invention can significantly reduce urea in serum after mice swimming
Content (P ＜ 0.01), and effect is substantially better than other G1～G2 Fructus Vitis viniferae composite powder；
11. conclusions
Above-mentioned experiment proves that the present invention Fructus Vitis viniferae composite powder containing probiotic bacteria can significantly improve immunity of organisms, improves mice
Muscle power and endurance, reduce urea in serum and the content of lactic acid after mouse movement, and can significantly improve glycogen in mouse liver
Deposit, contributes to alleviating the fatigue that sports load causes；The time that mice burden swimming to power exhausts can be extended.
Claims (10)
1. the Fructus Vitis viniferae composite powder containing probiotic bacteria, is mainly prepared by the raw material of following parts by weight: grape fruit powder 30-50 part, little
Fructus Hordei Germinatus powder 20-30 part, modified dietary fiber 8-15 part, papaya powder 6-12 part, Lactobacillus plantarum powder 11-17 part, ferment powder 11-17
Part, Fructus Citri Limoniae powder 5-10 part；
Described Lactobacillus plantarum powder is to prepare according to a conventional method with Lactobacillus plantarum CGMCC NO.11763 for starting strain.
2. the Fructus Vitis viniferae composite powder containing probiotic bacteria as claimed in claim 1, it is characterised in that main former by following parts by weight
Material preparation: grape fruit powder 35-45 part, Wheat Sprout Powder 23-27 part, modified dietary fiber 11-13 part, papaya powder 8-10 part, plant breast
Bacillus powder 13-15 part, ferment powder 13-15 part, Fructus Citri Limoniae powder 6-8 part.
3. the Fructus Vitis viniferae composite powder containing probiotic bacteria as claimed in claim 1, it is characterised in that main former by following parts by weight
Material preparation: grape fruit powder 40 parts, Wheat Sprout Powder 25 parts, modified dietary fiber 12 parts, papaya powder 9 parts, 14 parts of Lactobacillus plantarum powder, ferment
14 parts of powder of element, Fructus Citri Limoniae powder 7 parts.
4. the Fructus Vitis viniferae composite powder containing probiotic bacteria as described in claim 1-3 is arbitrary, it is characterised in that the preparation of described grape fruit powder
Method, comprises the following steps, and is put into by Fructus Vitis viniferae in the ultrasonic washing unit filling 0.1-0.3% sodium bicarbonate solution, in room temperature
Lower 200-400W, 35-45KHz clean 5-10min, drain, and each uva is arbitrarily cut into four parts, and single flat is layered on microwave and does
In power 3-5kW, temperature 120-130 DEG C in dry machine, it is dried 3-5s, is then immersed in the silk that mass percent concentration is 8-12%
8-10min in glue peptide solution, takes out, pulverizes immediately, ground product particle diameter 0.3-after-18--22 DEG C of freezing 30-50min
0.5mm, is subsequently added into the water of ground product quality 1-3 times, is 3.5-5.5 with breast acid for adjusting pH value, at room temperature in electric field intensity
25-35kV/cm, burst length 300-500 μ s, carry out high voltage pulse electric field processing 20-under the conditions of pulse frequency 200-300Hz
30min；Then under the conditions of power 150-300W, microwave irradiation and extraction 15-20min is carried out in room temperature, simultaneously at power 200-
300W, carries out ultrasonic assistant extraction under the conditions of frequency 30-40KHz；Add the mixed enzyme of extracting solution quality 1.5-2.5%, in
40-50 DEG C of enzymolysis 30-50min, enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are that 5-8% i.e. obtains Fructus Vitis viniferae
Powder；
Described mixed enzyme is that cellulase, protease, pectase, tannase 2-4:1-3:1-3:1-2 in mass ratio uniformly mixes.
5. the Fructus Vitis viniferae composite powder containing probiotic bacteria as described in claim 1-3 is arbitrary, it is characterised in that the system of described Wheat Sprout Powder
Preparation Method, comprises the steps: to be placed on by Semen Tritici aestivi in the ultrasonic washing unit equipped with 0.01-0.03% sodium bicarbonate solution in merit
Rate 200-400W, frequency 20-30KHz room temperature ultrasonic cleaning 3-5min, take out, drain, in frequency 2450MHz, power 3000W,
Temperature 38-42 DEG C, thickness of feed layer 2-4cm microwave drying 10-15s, then put temperature be 33-36 DEG C, pH value be the soak of 6-8
Middle immersion 4-6h, soak contains the compound enzyme that mass percent concentration is 0.15-0.25%, every 15-20min ventilation one
Secondary, ventilation pressure 0.13-0.15MPa, simultaneously in electric field intensity 10-15kV/cm, burst length 100-300 μ s, pulse frequency
60-80Hz condition carries out high voltage pulse electric field processing, until wheat water content content is 40-45%；Semen Tritici aestivi after immersion drains,
Carrying out dark germination in growing floor, dark germination temperature keeps 20-24 DEG C, and the dark germination time is 20-24h, and the Semen Tritici aestivi after germinateing is done
Dry is 5-8% to moisture, and low-temperature grinding, 60-80 mesh sieve excessively i.e. obtain Wheat Sprout Powder；
Described compound enzyme is that cellulase, 1,4 beta-glucanase, xylanase 2-4:2-4:1-3 in mass ratio uniformly mixes.
6. the Fructus Vitis viniferae composite powder containing probiotic bacteria as claimed in claim 1, it is characterised in that Lactobacillus plantarum powder viable bacteria content
For: 7 × 1012-9×1012cfu/g。
7. the Fructus Vitis viniferae composite powder containing probiotic bacteria as claimed in claim 1, it is characterised in that prepare during Lactobacillus plantarum powder freezing
Protectant preparation method, comprises the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, sharkskin collagen protein are respectively washed, are drained, presses
Mass ratio 8-10:3-5:2-4 uniformly mixes, and adds the lactic acid moistening 3-that pH value is 3.8-4.5 of mixed material quality 0.1-1 times
8h, pulverizes after-18--22 DEG C of freezing 1-2h immediately, freezing thickness of feed layer 3-5cm, ground product particle diameter 0.5-3mm, then
Add the water of ground product quality 10-20 times, be 3.5-5.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 25-35kV/
Cm, burst length 300-500 μ s, carry out high voltage pulse electric field processing 20-30min under the conditions of pulse frequency 200-300Hz；Then
Under the conditions of power 150-300W, microwave irradiation and extraction 15-20min is carried out, simultaneously in power 200-300W, frequency 30-in room temperature
Ultrasonic assistant extraction is carried out under the conditions of 40KHz；Add the compound enzyme of extracting solution quality 1-2%, in 45-55 DEG C of enzymolysis 30-
50min；Enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that 0.1-0.3mm i.e. obtains protective agent；
Described compound enzyme is cellulase, protease, amylase, pectase, tannase 2-4:1-3:1-3:1-2 in mass ratio:
1-2 uniformly mixes.
8. the Fructus Vitis viniferae composite powder containing probiotic bacteria as described in claim 1-3 is arbitrary, it is characterised in that described ferment powder preparation side
Method is as follows: the big grain things such as the fermentation liquid using pickle fermentation to produce discharge in latter stage is raw material, the dish leaf being filtered to remove in fermentation liquid
Matter obtains fermented liquid, and fermented liquid uses lyophilization to obtain ferment powder；Cryodesiccated condition is :-30--40 degree pre-freeze 6
Hour；Then lyophilizing is until water content is less than 5%.
9. as described in claim 1-8 is arbitrary, contain the preparation method of the Fructus Vitis viniferae composite powder of probiotic bacteria, it is characterised in that include as follows
Step: according to formula proportion, weighs each raw material, successively by ferment powder, Lactobacillus plantarum powder, grape fruit powder, Fructus Citri Limoniae powder, Fructus Tritici aestivi
Powder, papaya powder add V-type blending tank and uniformly mix 25-35min, speed of agitator 20-40r/min, are subsequently adding modified meals fine
Dimension uniformly mixed 12-18min, speed of agitator 40-60r/min, sterile filling, seal, pack the Fructus Vitis viniferae i.e. obtained containing probiotic bacteria and be combined
Powder.
10. contain the preparation method of the Fructus Vitis viniferae composite powder of probiotic bacteria as claimed in claim 9, it is characterised in that described modified meals
The preparation method of fiber, comprises the following steps: uniformly mixed by inulin, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 5-7:2-4:1-3 in mass ratio
Closing, add the water of its quality 3-7 times, room temperature 100-300W, 35-40KHz condition supersound extraction 10-15min, then at electric-field strength
Degree 20-40kV/cm, burst length 300-500 μ s, carry out high voltage pulse electric field processing 10-under the conditions of pulse frequency 200-400Hz
15min；It is 4.5-6.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis
20-48min；Enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are that 5-8% i.e. obtains modified dietary fiber；
Described enzyme is that cellulase, xylanase, laccase, pectase 1-3:1-3:1-2:1-2 in mass ratio uniformly mixes.
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