KR100440919B1 - Manufacture method of baidiomycetal mycelium contain germanium - Google Patents

Abstract

The present invention relates to a method for producing a mushroom mycelium containing germanium to maximize the function of the mushroom by containing germanium in the culture of the mushroom mycelium. The mushroom mycelium according to the present invention is characterized in that the mushroom mycelium has a high germanium content by culturing the mycelium in a medium containing germanium. As described above, the method of preparing the mushroom mycelium containing the germanium of the present invention is simple by manufacturing germanium in the preparation of the medium, and the mushroom mycelium containing the germanium has antitumor activity, immune enhancement, hypoglycemic activity, cardiac action, It is effective in the prevention of blood pressure lowering action and the prevention and treatment of various adult diseases and intractable adult diseases, so that the efficiency of user use can be maximized.

Description

Manufacture method of baidiomycetal mycelium contain germanium}

The present invention relates to a method for producing mushroom mycelium containing germanium. In general, about 22,000 species of mushrooms are known throughout the world, and about 10,000 are classified and identified among them, and about 2,000 are known to be available for food. Mushrooms have been widely used for food and medicinal purposes since ancient times.

The physiological activity of mushrooms is highly evaluated for its antitumor activity, immune boosting, hypoglycemic action, cardiovascular action, blood pressure lowering action and preventive effect against adult diseases such as cancer, stroke and heart disease. First of all, basidiomycete has long been taken as a food or medical treatment for a long time, and therefore, there are almost no side effects, and thus there is an advantage of not damaging other organs.

Since 1990, the production of mushrooms has increased rapidly, and as the standard of living increases, mushrooms, which have been used only for food, are increasing in the interest of modern people with the physiological activation function of mushrooms effective for various adult diseases. Reflecting the interests of modern people, the interest in germanium is also reaching its extreme.

The medical benefits of germanium were first discovered in 1930, after a report from Lourdes, a border between France and Spain, was effective in treating various diseases. From the analysis of the components, it is known that the germanium content is very high.

Since then, research on organic germanium that can act pharmacologically without remaining in the body has been actively conducted. Therefore, a relatively large amount of organic germanium is contained in medicinal herbs, such as ginseng, garlic, Ganoderma lucidum, and Mt. Research into the use of organo germanium in the treatment of refractory adult diseases such as cancer, hepatitis, rheumatoid arthritis, skin diseases, and aging continues. The role of organic germanium in vivo has been to enhance cellular oxygen supply, purify blood, stimulate the excretion of heavy metals in the body, activate NK cells and macrophage, induce inferon secretion, and induce T-lymphocyte Production control.

As such, mushrooms having excellent preventive effects against adult diseases and germanium effective for treating adult diseases are highly effective in their use. However, there is a problem in that the effects of the mushrooms and germanium for physiological activation of the human body cannot be encountered at the same time.

The present invention has been made to solve the above problems, to provide a method for producing a mushroom mycelium containing a germanium to contain the germanium at the same time in the culture of the mushroom mycelium so that the effect of the mushroom and germanium to provide have.

According to the present invention, in the method for producing a mushroom mycelium, by culturing the mycelium in a medium containing germanium by the method of producing a germanium-containing mushroom mycelium characterized in that it has a mushroom mycelium having a high germanium content do.

Here, the germanium content is preferably characterized in that more than 6000ppm.

The method of producing mushroom mycelium having a high germanium content comprises diluting germanium to 1000 ppm to form a medium suitable for bacteria to be inoculated, sterilizing the medium for at least 30 minutes at 121 ° C., and sterilizing the medium. Inoculating 10-15 ml of basidiomycete in 250-300 ml, and culturing the inoculated basidiomycetes at a predetermined temperature for 21 days or less.

In addition, in the culturing step, when the basidiomycete is Cordyceps sinensis, the culture at 20-25 ℃, and if the basidiomycetes ganoderma lucidum and pine mushroom and situation mushroom, it is preferable to culture at 27-29 ℃.

1 is a process chart for a method for producing mushroom mycelium containing germanium of the present invention.

Hereinafter, with reference to the accompanying drawings will be described in detail with respect to the manufacturing method of the mushroom mycelium containing germanium of the present invention.

The method for producing a mushroom mycelium containing germanium of the present invention comprises a step of culturing the medium after inoculation, sterilizing the basophilic bacteria.

In the medium composition step (M101) by diluting the inorganic germanium to 1000ppm to form a medium suitable for the bacteria to be inoculated. In other words, the germanium is diluted on a 1g / L basis, but due to the characteristics of germanium, it is not dissolved well, and is diluted with sufficient time. At this time, in selecting a medium suitable for the inoculation bacteria, Ganoderma lucidum and situation mushrooms using MYG medium, Matsutake mushrooms using ACM medium, Cordyceps sinensis is PDB (PDB) medium Selected and used.

The MYG medium composition table is as follows.

ingredient content Malt extract 1 g Yeast extract 0.4g Glucose (Dextrose) 0.4g Distilled water 100 ml

The ACM medium composition table is as follows.

ingredient content Yeast extract 0.6g Magnesium Sulfate 0.05g Potassium dihydrogenphosphate 0.046 g Dipotassium hydrogenphosphate 0.1g Soluble starch 2 g Bacto-soytone 0.4g Distilled water 100 ml

The PDB medium composition table is as follows.

ingredient content Potato (Photo, Infusion Solids) 0.4g Glucose (Dextrose) 2 g Distilled water 100 ml

In the sterilization step (M102), the prepared medium is sterilized at 121 ° C. for 15 minutes or more.

In the step of inoculating the basidiomycetes (M103), 10-15 ml of basidiomycetes are inoculated into 300-310 ml of sterilized medium.

In the step of culturing (M104), the inoculated basidiomycetes are cultured for less than 21 days at a predetermined temperature. At this time, the culture temperature is 27-29 ℃ if the basidiomycetes ganoderma lucidum and the situation mushroom, incubation at 27-29 ℃ if the basidiomycetes are Matsutake mushroom, at 20-25 ℃ for Cordyceps sinensis Incubate.

As such, the cultured culture solution was filtered to wash the filtered mycelium several times with distilled water, and then 0.1 g was taken. The nitric acid was added little by little while slowly increasing the temperature on the hot plate. After adjusting the 10ml with the third distilled water, ICP analysis was performed. First, within 0.3mg of the weight of the weighing saucer was first obtained, and 2g of the sample was accurately weighed on the weighing dish, and the lid of the weighing dish was opened and placed in a 105 ° C oven (Dry Oven) like a lid, followed by heating and drying for 4 hours. The balance plate was taken out of an oven (Dry Oven), placed in a desiccator, allowed to cool for 30 minutes, and then weighed on a scale. After drying again in an oven (Dry Oven) for 2 hours, it is cooled as before and weighed. Repeat until the weight is reduced and eventually becomes a dose.

Moisture (%) = (W1-W2) / (W1-W0) × 100

(W ₀ : Crucible weight (g))

(W ₁ : Weighing scale and sample (g))

(W ₂ : weight (g) until drying W ₁ to a constant weight)

Germanium content is expressed on a dry basis.

Dry basis (ppm) = 100 / (100-moisture content) x ICP analysis value (ppm)

First, Ganoderma lucidum cultured in a medium containing germanium by concentrationGanoderma lucidumTo determine the germanium content of mycelia, concentration analysis was carried out by dividing into shaking and political culture for 7 days, 14 days and 21 days. First, in shaking culture, as the concentration of germanium added to the medium increases,Ganoderma lucidumGermanium of mycelium The content was high. Also, as time goes by, each concentration shows a difference.Ganoderma lucidumThe amount of germanium in mycelium can be seen to be proportional to some extent..

Fig 1.

-◆-: 7 days culture

-■-: 14 days culture

-▲-: 21 days culture

Second, the analysis of germanium content according to the culture period of basidiomycetes is as follows.

(1) Ganoderma lucidum mycelium

After inoculation with Ganoderma lucidum, ICP analysis was carried out for static culture and shaking culture. The result is shown in Fig. 2. In general, the germanium content of the mycelium increased with time.

Fig 2.

-◆-: shake culture

(2) Paecilomyces japonica mycelium

Analysis results of Paecilomyces japonica mycelium are shown in Fig. 3. The germanium content is higher than any of the basophils analyzed in our laboratory. Overall, the contents of germanium were higher in the shake-cultured mycelium than in the political culture.

Fig 3.

-◆-: Political Culture

-▲-: shaking culture

(3) Phellinus igniarius mycelium

The analysis of mycelium (Phellinus igniarius) mycelium is shown in Fig. 4. In the case of situational mushrooms, there is a slight difference, but the shaking culture is higher than that of political culture.

Fig 4.

-◆-: Political Culture

-▲-: shaking culture

(4) Tricholoma matsutake mycelium

The results of analysis of mycelia of Tricholoma matsutake are shown in Fig. 5. In the case of pine mushroom, political culture was higher than shaking culture.

Fig 5.

-◆-: Political Culture

-▲-: shaking culture

As shown in the above table, as the concentration of germanium contained in the medium increased, the content of germanium of basidiomycete itself increased, and gradually increased over time until 21 days of incubation time. In addition, when viewing the germanium content of each strain, the germanium of Paecilomyces japonica mycelium was the highest content among the basidiomycetes used in the experiment. In view of the results so far, the optimum condition for the production of germanium-containing mushroom mycelium is cultured for 21 days in a culture medium shaken in 1000ppm germanium concentration using Paecilomyces japonica .

Cordyceps sinensis containing germanium cultured by such a manufacturing method has the effect of enhancing immune function, alleviating diseases of the heart system, lowering blood pressure, lowering cholesterol, inhibiting inflammation, and containing germanium Mushrooms protect eyes, prevent and treat diseases of the respiratory system, prevent and cure diseases of the digestive system, and have no side effects.

The production method of the mushroom mycelium containing germanium of the present invention is a simple method of manufacturing by containing germanium at the time of forming the medium, the mushroom mycelium containing germanium has antitumor activity, immune enhancement, hypoglycemic activity, cardiovascular action, blood pressure lowering action and Since it is effective in the prevention and treatment of various adult diseases and intractable adult diseases, it is a very excellent invention to maximize the efficiency of use of the user.

Claims (10)

A medium containing 6 g / L of yeast extract, 0.5 g / L of magnesium sulfate, 0.46 g / L of potassium dihydrogen phosphate, 1 g / L of dipotassium hydrogen phosphate, 20 g / L of soluble starch, 4 g / L of bacto-sodium and 1000 ppm of germanium Formulating; Sterilizing the medium at 121 ° C. for at least 15 minutes; Inoculating 10-15 ml of basidiomycetes in 300-310 ml of the sterilized medium; Germanium-containing mushroom mycelium production method comprising the step of culturing the inoculated basidiomycete at less than 21 days at 27-29 ℃. delete delete The method of claim 1, The mushroom mycelium is a mushroom mycelium manufacturing method containing germanium, characterized in that the pine mushroom. delete Formulating a medium containing 4 g / L potato, 20 g / L glucose and 1000 ppm germanium; Sterilizing the medium at 121 ° C. for at least 15 minutes; Inoculating 10-15 ml of basidiomycetes in 300-310 ml of the sterilized medium; Germanium-containing mushroom mycelium production method comprising the step of culturing the inoculated basidiomycetes at less than 21 days at 24-26 ℃. The method of claim 6, The mushroom mycelium is a method for producing mushroom mycelium containing germanium, characterized in that the cordyceps. Forming a medium containing 10 g / L of malt extract, 4 g / L of yeast extract, 4 g / L of glucose and 1000 ppm of germanium; Sterilizing the medium at 121 ° C. for at least 15 minutes; Inoculating 10-15 ml of basidiomycetes in 300-310 ml of the sterilized medium; Germanium-containing mushroom mycelium production method comprising the step of culturing the inoculated basidiomycete at less than 21 days at 27-29 ℃. The method of claim 8, The mushroom mycelium is a mushroom mycelium manufacturing method containing germanium, characterized in that the ganoderma lucidum or situation mushroom. Mushroom mycelium containing germanium prepared by any one of claims 1 to 9.