CN105622256A - Cultivation method of ganoderma lucidum antler substitute material - Google Patents
Cultivation method of ganoderma lucidum antler substitute material Download PDFInfo
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- CN105622256A CN105622256A CN201510982160.5A CN201510982160A CN105622256A CN 105622256 A CN105622256 A CN 105622256A CN 201510982160 A CN201510982160 A CN 201510982160A CN 105622256 A CN105622256 A CN 105622256A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a cultivation method of a ganoderma lucidum antler substitute material. The method comprises the following steps: earlier stage processing of wood chips; batching, mixing, and stacking and fermentation: batching according to the formula of a culture medium, to be specific, firstly uniformly mixing wood chips, auxiliary materials, gypsum and sugar to form dry materials, adding a nutrient solution and sufficiently and uniformly stirring to form a culture medium substrate, then performing stacking and fermentation after stirring to ensure that the nutrient solution is sufficiently absorbed by the culture medium substrate, and then bagging; sterilizing; inoculating; performing lucid ganoderma growth management; bud thinning stage management; harvesting: timely harvesting when ganoderma lucidum antler is mature. According to the method, the material-water ratio is controlled through the specially-made nutrient solution, the nutritive value of obtained ganoderma lucidum antler is high, and the polysaccharide content of the ganoderma lucidum antler is greatly improved compared with that of the ganoderma lucidum antler cultured by the prior art; besides, the nutrient solution adopted by the method, particularly plant raw material components used are subjected to steam explosion, distillation, and high shear homogenizer processing, so that nano-emulsion type plant oily components which are good in compatibility with other components in the culture medium are obtained, and the proliferation of various cells is further stimulated.
Description
Technical field
The present invention relates to field of edible fungus culture, particularly relate to a kind of Antler Mythic Fungus substituting stuff cultivation method.
Background technology
Glossy ganoderma (formal name used at school: GanodermaLucidumKarst), also known as Ganoderma lucidum seu Japonicum, celestial grass, have another name called red sesame, red sesame, Ganoderma Lucidum, Ganoderma, 10000 years gill fungus, Ganoderma lucidum seu Japonicumes, is the red sesame of polyporaceae plant or the complete stool of purple sesame, is the sporophore of polyporaceae fungi glossy ganoderma. Profile is umbrella shape, cap kidney shape, semicircle or subcircular. Glossy ganoderma, as the Chinese tradition valuable ingredient of traditional Chinese medicine having thousands of years medicinal histories, has invigorating QI and tranquilization, relieving cough and asthma effect, and for dizzy egersis, shortness of breath and palpitation, empty labor is coughed and breathed heavily. Glossy ganoderma possesses very high pharmaceutical use, and the modern pharmacology research through scientific research institution's many decades confirms, glossy ganoderma, for enhancing body immunity, regulates blood sugar, control blood pressure, auxiliary tumor chemoradiotherapy, and liver protecting promotes that the aspects such as sleep all have significant curative effect. Ganoderan is one of principle active component of glossy ganoderma medicinal function; mainly it is present in glossy ganoderma cell wall inwall; major part is beta-glucan; its monose forms except containing except a large amount of glucose; also containing a small amount of pectinose, wood sugar, Fucose; rhamnosyl; semi-lactosi and seminose etc.; they connect mainly with the glycosidic link such as (1-3), (1-4) and (1-6) greatly; there is biological activity widely, such as strengthening immunity, remove human free radical, protect cardiovascular, radioprotective, protection liver, the effect such as antitumor. Triterpenes is also one of effective constituent of glossy ganoderma, and triterpenes contained by glossy ganoderma not lower over one hundred kind, wherein based on tetracyclic triterpenes, the bitter taste of glossy ganoderma is relevant with contained triterpenes, human hepatoma cell is had cytotoxicity, also can suppress the release of histamine, have and protect the effect such as liver and antianaphylaxis.
Antler Mythic Fungus is that likeness in form has the glossy ganoderma of a pair as deer horn body, it is undifferentiated cap in process of growth, it is different from the pileus ganoderma lucidum that other are general, do not distribute spore powder, therefore not consuming parent nutrition, nutrition is retained in sporophore completely, thus Antler Mythic Fungus sporophore contain polysaccharide, ergosterol, N.F,USP MANNITOL, adenosine, triamino acid etc. effective ingredient pileus ganoderma lucidum more common than other content high, being the superfine product in glossy ganoderma, every index content is all considerably beyond sheet sesame. Such as, it is possible to the content of organic germanium improving human body cell resistance of oxidation, up to 1680 to 2000ppm, is 3 to 4 times of sheet sesame, and the contained macromolecule polysaccharide body that can strengthen immune function of human body is 43%, is more than 5 times of sheet sesame. Not only pharmaceutical use height, and Antler Mythic Fungus and sheet sesame are carried out live body grafting, cultivate glossy ganoderma dish garden product innovation, more allow Antler Mythic Fungus have a sudden rise in social status.
Antler Mythic Fungus is adapted at growing containing in the matrix such as grape glucose, sucrose, starch, Mierocrystalline cellulose, xylogen. The agricultural byproducts such as general wood chip, cotton seed hulls and corn cob carry out producing cultivation as substratum. Owing to region is different, by the impact of starting material factor, Antler Mythic Fungus is produced has certain limitation, it is difficult to extensively carry out.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that the Antler Mythic Fungus substituting stuff cultivation substratum of a kind of with low cost, successful.
The present invention is achieved by the following technical solution:
A kind of Antler Mythic Fungus substituting stuff cultivation method, comprises the steps:
1) wood chip processing in early stage: pulverize after the trunk of trees after felling and branch are removed the peel, dry for subsequent use;
2) batching, spice, windrow ferment: prepare burden according to the formula of substratum, first by wood chip, auxiliary material, gypsum, the mixed even formation siccative of sugar, then Ensure Liquid liquid is fully mixed thoroughly and is formed substratum matrix, after spice terminates, windrow ferments, nutritive medium is fully absorbed by substratum matrix, can pack;
3) sterilizing: put into sterilizing device, high pressure steam sterilization or normal-pressure sterilization by having filled Bag Material bag, material bag is moved into cooling room after terminating by sterilizing, treats that temperature is down to less than 30 DEG C, can inoculate;
4) inoculate: by Antler Mythic Fungus strain inoculation in substratum, then the bacterium bag after inoculation is put into culturing room and cultivate, treat that mycelia covers with bacterium bag, proceed to fertility room after the mycelia kink variable color of surface and carry out sesame period management;
5) sesame period management is gone out: control room temp, humidity, CO2Concentration, intensity of illumination, pH value;
6) flower bud period management is dredged: in children's flower bud phase, manually remove small and weak sesame body, leave and take sturdy sesame body;
7) gather: gather in time when Antler Mythic Fungus is ripe;
Step 1) described in substratum, be made up of the raw material of following weight parts: wood chip 35-70 part, auxiliary material 25-60 part, gypsum 0.5-1.5 part, sugar 0.1-1 part, with nutritive medium control material-water ratio be 1:1.3-1.5;
Described nutritive medium is made up of following part by weight composition:
PH is adjusted to be 6.0 with phosphate buffered saline buffer;
Described promotor adopts following method to prepare:
1. Feedstock treating: Herba Centellae is clean, drying, pulverizing;
2. Steam explosion treatment: steam explosion tank put into by material step 1. obtained, burstpressures 3.5Mpa, the dwell time maintains 200s;
3. distillation process: distiller put into by material step 2. obtained, bottom distiller, heating flame or lead to into steam, when hot steam is filled in distiller, water vapour, by prolong, is introduced in condenser, then by water-and-oil separator, collects essence oil;
4. high-shear homogenizing machine process: essence oil step 3. obtained is processed by high-shear homogenizing machine, and linear velocity 30m/s, time 5min, makes nano-emulsion, and obtains with filtering with microporous membrane is degerming.
Step 1 of the present invention) described in pulverizing, it is preferable that being crushed to particle diameter is 0.5-1.0cm; Described substratum is made up of the raw material of following weight parts: wood chip 42 parts, auxiliary material 53 parts, 1 part, gypsum, sugar 1 part, with nutritive medium control material-water ratio be 1:1.3-1.5; Described wood chip preferably derives from maple wood and vertebra wood; Described auxiliary material is the compositions of one or more in cotton seed hulls, wheat bran, Semen Maydis powder, when used as a mixture, it is possible to be arbitrary proportion.
Step 2) described in windrow fermentation, preferably control fermentation heap internal temperature is 65-70 DEG C, fermentation time is 1-2h, described pack, it is preferable that 17X35X0.05 specification acrylic plastering goes out mushroom bag, feeds about 400 grams, charging should not be expired very much, material bag two Zha Kou, bag mouth notes cleaning, and prevents later stage living contaminants;
Step 3) described in high pressure steam sterilization, it is preferable that 121 DEG C of sterilizings 1.5-2 hour, normal-pressure sterilization, it is preferable that 100 DEG C of sterilizings 10-12 hour.
Step 4) described in inoculation, it is preferable that according to the inoculum size of 1-3% (w/w), described culturing room require cleaning, clean, ventilate, between bacterium bag, temperature remains between 22-25 DEG C, relative air humidity remains on 55-60, cultivates, generally cultivate 30-40d under non-illuminated conditions.
Step 5) described in go out sesame period management, it is preferable that control room temp 24-28 DEG C, humidity 75-85%, CO2Concentration 0.5-0.9, adopts fixing light source, and intensity of illumination 300-4001x, pH value is 5.3-6.7.
Step 6) described in thin flower bud period management, it is preferable that leave and take sturdy sesame body 1-2.
Compared with prior art, tool of the present invention has the following advantages:
1, in prior art, the substituting stuff cultivation substratum of preparation glossy ganoderma, control material-water ratio, it is generally adopt tap water or pure water, the present invention adopts special nutritive medium to control material-water ratio, the nutritive value height of the Antler Mythic Fungus obtained, polysaccharide content significantly increases than prior art, and output increases by more than 15%.
2, the nutritive medium that the present invention is used, by adding trace metal ion and plant promoting agent, it is possible to stimulate the propagation of various kinds of cell. The plant material composition particularly used have passed through steam explosion, distillation, high-shear homogenizing machine process, obtains the plant oil components of nanometer emulsus, and in substratum, the consistency of other formulas is good.
Embodiment
With embodiment, the invention will be further described below, but the present invention is not limited to these embodiments.
Embodiment 1-3:
A kind of Antler Mythic Fungus substituting stuff cultivation method, comprises the steps:
1) wood chip processing in early stage: being crushed to particle diameter after the trunk of trees after felling and branch being removed the peel is 0.5-1.0cm, dries for subsequent use;
2) batching, spice, windrow ferment: prepare burden according to the formula of substratum, first by wood chip, auxiliary material, gypsum, the mixed even formation siccative of sugar, then Ensure Liquid liquid is fully mixed thoroughly and is formed substratum matrix, after spice terminates, windrow ferments, and control fermentation heap internal temperature is 65-70 DEG C, and fermentation time is 1-2h, nutritive medium is fully absorbed by substratum matrix, can pack;
3) sterilizing: put into sterilizing device, high pressure steam sterilization or normal-pressure sterilization by having filled Bag Material bag, material bag is moved into cooling room after terminating by sterilizing, treats that temperature is down to less than 30 DEG C, can inoculate;
4) inoculate: Antler Mythic Fungus bacterial classification is inoculated in substratum according to the inoculum size of 1-3% (w/w), then the bacterium bag after inoculation is put into culturing room to cultivate, culturing room requires cleaning, clean, ventilation, between bacterium bag, temperature remains between 22-25 DEG C, relative air humidity remains on 55-60, cultivates, generally cultivate 30-40d under non-illuminated conditions, treat that mycelia covers with bacterium bag, proceed to fertility room after the mycelia kink variable color of surface and carry out sesame period management;
5) sesame period management is gone out: control room temp 24-28 DEG C, humidity 75-85%, CO2Concentration 0.5-0.9, adopts fixing light source, intensity of illumination 300-4001x, and pH value is 5.3-6.7;
6) dredge flower bud period management: in children's flower bud phase, manually remove small and weak sesame body, leave and take sturdy sesame body 1-2;
7) gather: gather in time when Antler Mythic Fungus is ripe;
Step 1) described in substratum, be made up of the raw material of following weight parts: wood chip 35-70 part, auxiliary material 25-60 part, gypsum 0.5-1.5 part, sugar 0.1-1 part, with nutritive medium control material-water ratio be 1:1.3-1.5;
Described nutritive medium is made up of following part by weight composition:
PH is adjusted to be 6.0 with phosphate buffered saline buffer;
Described promotor adopts following method to prepare:
1. Feedstock treating: Herba Centellae is clean, drying, pulverizing;
2. Steam explosion treatment: steam explosion tank put into by material step 1. obtained, burstpressures 3.5Mpa, the dwell time maintains 200s;
3. distillation process: distiller put into by material step 2. obtained, bottom distiller, heating flame or lead to into steam, when hot steam is filled in distiller, water vapour, by prolong, is introduced in condenser, then by water-and-oil separator, collects essence oil;
4. high-shear homogenizing machine process: essence oil step 3. obtained is processed by high-shear homogenizing machine, and linear velocity 30m/s, time 5min, makes nano-emulsion, and obtains with filtering with microporous membrane is degerming.
The cultivating method reference example of comparative example.
The composition analysis of the Antler Mythic Fungus that employing embodiment and comparative example obtain:
Result shows, the Antler Mythic Fungus nutritive ingredient that the embodiment of the present invention obtains is significantly higher than comparative example.
Claims (4)
1. an Antler Mythic Fungus substituting stuff cultivation method, it is characterised in that, comprise the steps:
1) wood chip processing in early stage: pulverize after the trunk of trees after felling and branch are removed the peel, dry for subsequent use;
2) batching, spice, windrow ferment: prepare burden according to the formula of substratum, first by wood chip, auxiliary material, gypsum, the mixed even formation siccative of sugar, then Ensure Liquid liquid is fully mixed thoroughly and is formed substratum matrix, after spice terminates, windrow ferments, nutritive medium is fully absorbed by substratum matrix, can pack;
3) sterilizing: put into sterilizing device, high pressure steam sterilization or normal-pressure sterilization by having filled Bag Material bag, material bag is moved into cooling room after terminating by sterilizing, treats that temperature is down to less than 30 DEG C, can inoculate;
4) inoculate: by Antler Mythic Fungus strain inoculation in substratum, then the bacterium bag after inoculation is put into culturing room and cultivate, treat that mycelia covers with bacterium bag, proceed to fertility room after the mycelia kink variable color of surface and carry out sesame period management;
5) sesame period management is gone out: control room temp, humidity, CO2Concentration, intensity of illumination, pH value;
6) flower bud period management is dredged: in children's flower bud phase, manually remove small and weak sesame body, leave and take sturdy sesame body;
7) gather: gather in time when Antler Mythic Fungus is ripe;
Step 1) described in substratum, be made up of the raw material of following weight parts: wood chip 35-70 part, auxiliary material 25-60 part, gypsum 0.5-1.5 part, sugar 0.1-1 part, with nutritive medium control material-water ratio be 1:1.3-1.5;
Described nutritive medium is made up of following part by weight composition:
PH is adjusted to be 6.0 with phosphate buffered saline buffer;
Described promotor adopts following method to prepare:
1. Feedstock treating: Herba Centellae is clean, drying, pulverizing;
2. Steam explosion treatment: steam explosion tank put into by material step 1. obtained, burstpressures 3.5Mpa, the dwell time maintains 200s;
3. distillation process: distiller put into by material step 2. obtained, bottom distiller, heating flame or lead to into steam, when hot steam is filled in distiller, water vapour, by prolong, is introduced in condenser, then by water-and-oil separator, collects essence oil;
4. high-shear homogenizing machine process: essence oil step 3. obtained is processed by high-shear homogenizing machine, and linear velocity 30m/s, time 5min, makes nano-emulsion, and obtains with filtering with microporous membrane is degerming.
2. a kind of Antler Mythic Fungus substituting stuff cultivation method according to claim 1, it is characterised in that: step 1) described in substratum be made up of the raw material of following weight parts: wood chip 42 parts, auxiliary material 53 parts, 1 part, gypsum, sugar 1 part, to control material-water ratio with nutritive medium be 1:1.3-1.5.
3. a kind of Antler Mythic Fungus substituting stuff cultivation method according to claim 1, it is characterised in that: step 1) described in wood chip derive from maple wood and vertebra wood.
4. a kind of Antler Mythic Fungus substituting stuff cultivation method according to claim 1, it is characterised in that: step 5) described in go out sesame period management, control room temp 24-28 DEG C, humidity 75-85%, CO2Concentration 0.5-0.9, adopts fixing light source, and intensity of illumination 300-4001x, pH value is 5.3-6.7.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107197692A (en) * | 2017-06-08 | 2017-09-26 | 绿宝生态农业（漳州）有限公司 | A kind of cultural method of Ganoderma lucidum |
| CN111512883A (en) * | 2019-02-02 | 2020-08-11 | 李志广 | Method for planting ornamental ganoderma lucidum antler and paint |
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| CN111512883A (en) * | 2019-02-02 | 2020-08-11 | 李志广 | Method for planting ornamental ganoderma lucidum antler and paint |
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Application publication date: 20160601 |