CN106866235A - A kind of Antrodia camphorata ware training culture medium and preparation method thereof - Google Patents
A kind of Antrodia camphorata ware training culture medium and preparation method thereof Download PDFInfo
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- CN106866235A CN106866235A CN201710080733.4A CN201710080733A CN106866235A CN 106866235 A CN106866235 A CN 106866235A CN 201710080733 A CN201710080733 A CN 201710080733A CN 106866235 A CN106866235 A CN 106866235A
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- culture medium
- antrodia camphorata
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention discloses a kind of Antrodia camphorata ware training culture medium and preparation method thereof.It is made up of the raw material of following parts by weight, potato leaches 200 300 parts of powder, 20 40 parts of glucose, 0 20 parts of soluble soybean powder, 1 20 parts of malt extract, 0.5 1.5 parts of magnesium sulfate, 10 60 parts of cinnamon mixture, 0.5 1.5 parts of potassium dihydrogen phosphate, 0.01 0.05 parts of vitamin B1,0 0.1 parts of chloramphenicol, 15 25 parts of agar, 1000 parts of ultra-pure water.Culture medium of the present invention cultivates Antrodia camphorata with culture dish, and condition of culture controllability is strong, and simplified culture program can reduce living contaminants, it also avoid heavy metal pollution, can also improve the increment of Cinnamomum kanahirai hay camphorata mycelium or fructification, reduces production cost.
Description
Technical field
The invention belongs to fungal culture technical field, and in particular to a kind of Antrodia camphorata ware training culture medium and preparation method thereof.
Background technology
Mushroom or red camphor tree mushroom etc. in Antrodia camphorata (Antrodia camphorata) alias antrodia, cinnamomum kanahirai hay mushroom, camphor tree, are under the jurisdiction of load
Daughter bacteria guiding principle, Aphyllophorales, Polyporaceae, antrodia karst is that the food (medicine) for being found in TaiWan, China the nineties in 20th century uses fungi,
There is the good reputation of " forest ruby ".Antrodia camphorata has various physiologically active ingredients, including polysaccharide, steroid, triterpenes, adenosine
Class, unrighted acid and immune protein etc..Research in recent years confirms that antrodia has Antialcoholic liver-protecting, elimination tumour, kidney tonifying strong
The effects such as the heart, the whole intestines of beneficial stomach, reinforced immunological, analgesia antibacterial, especially have remarkable efficacy in terms for the treatment of liver cancer and cervix cancer.
Antrodia camphorata is wood-destroying fungi.Wild antrodia is only grown in Cinnamomum kanahirai hay (Cinnamomum more than a century
The cinnamomum kanehirai timber moist surface of kanehirae) the rotten heartwood inwall of trunk, or withered lodging.Wild Antrodia camphorata growth speed
Slowly, quantity is very rare, its price once promotion to 15000 dollars/kg for degree.Cinnamomum kanehirai is slow-growing, can grow ox
The veteran of antrodia is very rare, and Antrodia camphorata resource far can not meet the market demand, thus the artificial cultivation of Antrodia camphorata just has
Important meaning.
Manually culture has the training formula culture of liquid culture, solid state rheology, cultivation basswood and ware to antrodia.Ware trains formula cultivation with fine jade
Fat is solid content and adds appropriate nutrient source as matrix, coordinates good quality strain to be regulated and controled under appropriate circumstances, allows ox
Antrodia is grown on culture dish.Ware trains formula culture using culture dish as culture vessel, and condition of culture controllability is strong, can not only reduce
Miscellaneous bacteria cross pollution between unit, it also avoid heavy metal pollution, help to increase the yield of unit cultivated area, reduce production
Cost, it is easy to accomplish quantify production, but, current ware trains the antrodia cultivated on the culture medium of formula culture and there is the speed of growth
Slowly, easy infection miscellaneous bacteria, yields poorly, and is not suitable for the defects such as large-scale production.
The content of the invention
It is an object of the invention to provide a kind of Antrodia camphorata ware training culture medium and preparation method thereof.
A kind of Antrodia camphorata ware trains culture medium, is made up of the raw material of following parts by weight, and potato leaches 200-300 parts of powder,
Glucose 20-40 parts, 0-20 parts of soluble soybean powder, malt extract 1-20 parts, magnesium sulfate 0.5-1.5 parts, cinnamon is mixed
Compound 10-60 parts, potassium dihydrogen phosphate 0.5-1.5 parts, vitaminB10 .01-0.05 parts, chloramphenicol 0-0.1 parts, agar 15-25
Part, 1000 parts of ultra-pure water.
The preparation method of the malt extract is：Malt, plus the 4-6 times of deionized water of parts by weight are crushed, 0.5mm is used
Strainer filtering, at room temperature gentle agitation mix 2h, 6000g, 30min, remove supernatant, with hydrochloric acid adjust pH to 7.2,121 DEG C of high pressures
Sterilizing 15min, freeze-drying is into powder.
The preparation method of the cinnamon mixture is：Cinnamon is first ground into powder, and 60 DEG C of drying are added ultrapure
121 DEG C of extraction 20min of water, extraction is repeated once after filtering, is made.
The cinnamon is one or more in camphor tree, Cinnamomum kanahirai hay, cinnamomum camphora, yellow camphor tree, great Ye camphor trees, heavy water camphor tree, Chinese cassia tree.
The Antrodia camphorata ware training medium pH is 4.5-5.5.
The Antrodia camphorata ware training culture medium is additionally added 10-20 parts of crisp Ye Jinmao moss extract.
The preparation method of the crisp Ye Jinmao moss extract is：Crisp Ye Jinmao moss crushed, plus 4-6 times of parts by weight go
Ionized water, with the strainer filtering of 0.5mm, gentle agitation mixing 2h, 6000g, 30min, remove supernatant at room temperature, and pH is adjusted extremely with hydrochloric acid
7.2,121 DEG C of autoclaving 15min, freeze-drying is into powder.
Above-mentioned Antrodia camphorata ware trains the preparation method of culture medium, carries out in accordance with the following steps：Cinnamon mixture is first prepared,
Then each component dissolving is mixed, and adjusts pH to 4.5-5.5,121 DEG C of autoclaving 20min, and the culture medium after sterilizing is in ultra-clean work
Platform is down flat plate, per plate 20-30ml culture mediums, after inoculation Antrodia camphorata strain after culture medium solidifying.
Beneficial effects of the present invention：Culture medium of the present invention cultivates Antrodia camphorata with culture dish, and condition of culture controllability is strong, letter
Change culture program, living contaminants can be reduced, it also avoid heavy metal pollution, can also improve the life of Cinnamomum kanahirai hay camphorata mycelium or fructification
Amount long, reduces production cost.
Specific embodiment
With reference to specific embodiment, the present invention will be further described.
In following embodiments：
The preparation method of the malt extract is：Malt, plus 5 times of deionized waters of parts by weight are crushed, with 0.5mm's
Strainer filtering, gentle agitation mixing 2h, 6000g, 30min, remove supernatant at room temperature, adjust pH to 7.2 with hydrochloric acid, and 121 DEG C of high pressures are gone out
Bacterium 15min, freeze-drying is into powder.
The preparation method of the cinnamon mixture is：Cinnamon is first ground into powder, and 60 DEG C of drying are added ultrapure
121 DEG C of extraction 20min of water, extraction is repeated once after filtering, is made.The cinnamon is camphor tree, Cinnamomum kanahirai hay, cinnamomum camphora, yellow camphor tree, big
One or more in leaf camphor tree, heavy water camphor tree, Chinese cassia tree.
The preparation method of the crisp Ye Jinmao moss extract is：Crisp Ye Jinmao moss is crushed, plus 5 times of parts by weight go from
Sub- water, with the strainer filtering of 0.5mm, gentle agitation mixing 2h, 6000g, 30min, remove supernatant at room temperature, and pH is adjusted extremely with hydrochloric acid
7.2,121 DEG C of autoclaving 15min, freeze-drying is into powder.
The preparation method of navel grass extract is：Navel grass meal is broken, plus 5 times of deionized waters of parts by weight, use 0.5mm
Strainer filtering, at room temperature gentle agitation mix 2h, 6000g, 30min, remove supernatant, with hydrochloric acid adjust pH to 7.2,121 DEG C of high pressures
Sterilizing 15min, freeze-drying is into powder.
Embodiment 1
A kind of Antrodia camphorata ware trains culture medium, is made up of the following raw material, and potato leaches powder 250g, glucose 30g, soluble
Soy meal 10g, malt extract 10g, magnesium sulfate 1g, cinnamon mixture 30g, potassium dihydrogen phosphate 1g, vitamin B1
0.03g, chloramphenicol 0.05g, agar 20g, ultra-pure water 1L.
Cinnamon mixture is first prepared, then each component dissolving is mixed, adjust pH to 5.0,121 DEG C of autoclaving 20min,
Culture medium after sterilizing is down flat plate in superclean bench, per plate 25ml culture mediums, after inoculation Antrodia camphorata strain after culture medium solidifying,
Cultivated under the conditions of 25 DEG C 140 days and harvest mycelium or fructification.
Embodiment 2
A kind of Antrodia camphorata ware trains culture medium, is made up of the following raw material, and potato leaches powder 200g, glucose 20g, soluble
Soy meal 2g, malt extract 3g, magnesium sulfate 0.5g, cinnamon mixture 10g, potassium dihydrogen phosphate 0.5g, vitamin B1
0.01g, chloramphenicol 0.01g, agar 15g, ultra-pure water 1L.
Cinnamon mixture is first prepared, then each component dissolving is mixed, adjust pH to 4.5,121 DEG C of autoclaving 20min,
Culture medium after sterilizing is down flat plate in superclean bench, per plate 20ml culture mediums, after inoculation Antrodia camphorata strain after culture medium solidifying,
Cultivated under the conditions of 20 DEG C 180 days and harvest mycelium or fructification.
Embodiment 3
A kind of Antrodia camphorata ware trains culture medium, is made up of the following raw material, and potato leaches powder 300g, glucose 40g, soluble
Soy meal 20g, malt extract 20g, magnesium sulfate 1.5g, cinnamon mixture 60g, potassium dihydrogen phosphate 1.5g, vitamin B1
0.05g, chloramphenicol 0.1g, agar 25g, ultra-pure water 1L.
Cinnamon mixture is first prepared, then each component dissolving is mixed, adjust pH to 5.5,121 DEG C of autoclaving 20min,
Culture medium after sterilizing is down flat plate in superclean bench, per plate 30ml culture mediums, after inoculation Antrodia camphorata strain after culture medium solidifying,
Cultivated under the conditions of 28 DEG C 90 days and harvest mycelium or fructification.
Embodiment 4
A kind of Antrodia camphorata ware trains culture medium, is made up of the following raw material, and potato leaches powder 220g, glucose 28g, soluble
Soy meal 8g, malt extract 8g, magnesium sulfate 0.8g, cinnamon mixture 35g, crisp Ye Jinmao moss extract 15g, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.9g, vitamin B1 0.03g, chloramphenicol 0.06g, agar 20g, ultra-pure water 1L.
Cinnamon mixture is first prepared, then each component dissolving is mixed, adjust pH to 4.8,121 DEG C of autoclaving 20min,
Culture medium after sterilizing is down flat plate in superclean bench, per plate 22ml culture mediums, after inoculation Antrodia camphorata strain after culture medium solidifying,
Cultivated under the conditions of 25 DEG C 120 days and harvest mycelium or fructification.
Embodiment 5
A kind of Antrodia camphorata ware trains culture medium, is made up of the following raw material, and potato leaches powder 250g, glucose 30g, soluble
Soy meal 10g, malt extract 10g, navel 10 parts of extract of grass, magnesium sulfate 1g, cinnamon mixture 30g, potassium dihydrogen phosphate
1g, vitamin B1 0.03g, chloramphenicol 0.05g, agar 20g, ultra-pure water 1L.
Cinnamon mixture is first prepared, then each component dissolving is mixed, adjust pH to 5.0,121 DEG C of autoclaving 20min,
Culture medium after sterilizing is down flat plate in superclean bench, per plate 25ml culture mediums, after inoculation Antrodia camphorata strain after culture medium solidifying,
Cultivated under the conditions of 25 DEG C 140 days and harvest mycelium or fructification.
Comparative example 1
A kind of Antrodia camphorata ware trains culture medium, is made up of the following raw material, and potato leaches powder 250g, glucose 30g, soluble
Soy meal 10g, magnesium sulfate 1g, cinnamon mixture 30g, potassium dihydrogen phosphate 1g, vitamin B1 0.03g, chloramphenicol 0.05g,
Agar 20g, ultra-pure water 1L.
Cinnamon mixture is first prepared, then each component dissolving is mixed, adjust pH to 5.0,121 DEG C of autoclaving 20min,
Culture medium after sterilizing is down flat plate in superclean bench, per plate 25ml culture mediums, after inoculation Antrodia camphorata strain after culture medium solidifying,
Cultivated under the conditions of 25 DEG C 140 days and harvest mycelium or fructification.
Detection example：
The total content of Antrodia camphorata fructification triterpene compound is determined using vanilla root rot development process；Using benzene
Phenol-sulfuric acid method determines polyoses content in Antrodia camphorata fructification, and testing result is as shown in table 1：
Table 1
| Detection example | The total content of triterpene compound | Polyoses content |
| Embodiment 1 | 35mg/g | 4.6mg/g |
| Embodiment 2 | 33mg/g | 4.5mg/g |
| Embodiment 3 | 34mg/g | 4.4mg/g |
| Embodiment 4 | 55mg/g | 7.4mg/g |
| Embodiment 5 | 56mg/g | 7.2mg/g |
| Comparative example 1 | 18mg/g | 2.5mg/g |
As can be seen from Table 1, the total content and polyoses content of Antrodia camphorata fructification triterpene compound are equal in embodiment 1-5
It is higher, it is much higher than comparative example 1, without malt extract is added in comparative example 1, other conditions are same as Example 1, it was demonstrated that wheat
Bud extract serves key effect herein, and the total content and polyoses content of the triterpene compound of embodiment 4-5 are higher than implementation
Example 1-3, because adding crisp Ye Jinmao moss extract or navel grass extract, it was demonstrated that above-mentioned substance serves key effect herein.
Embodiments of the invention are the foregoing is only, the scope of the claims of the invention is not thereby limited, it is every to utilize this hair
Any modification, equivalent substitution and improvements that bright description is made etc., or directly or indirectly it is used in other related technology necks
Domain, is included within the scope of the present invention.
Claims (8)
1. a kind of Antrodia camphorata ware trains culture medium, it is characterised in that be made up of the raw material of following parts by weight, and potato leaches powder
200-300 parts, glucose 20-40 parts, 0-20 parts of soluble soybean powder, malt extract 1-20 parts, magnesium sulfate 0.5-1.5 parts,
10-60 parts of cinnamon mixture, potassium dihydrogen phosphate 0.5-1.5 parts, vitamin B1 0.01-0.05 parts, chloramphenicol 0-0.1 parts,
Agar 15-25 parts, 1000 parts of ultra-pure water.
2. Antrodia camphorata ware according to claim 1 trains culture medium, it is characterised in that the preparation method of the malt extract
For：Crush malt, plus the 4-6 times of deionized water of parts by weight, with the strainer filtering of 0.5mm, gentle agitation mixes 2h at room temperature,
6000g, 30min, remove supernatant, and pH is adjusted to 7.2 with hydrochloric acid, 121 DEG C of autoclaving 15min, and freeze-drying is into powder.
3. Antrodia camphorata ware according to claim 1 trains culture medium, it is characterised in that the preparation of the cinnamon mixture
Method is：Cinnamon is first ground into powder, and 60 DEG C of drying add 121 DEG C of extraction 20min of ultra-pure water, repeat to extract after filtering
Once, it is made.
4. Antrodia camphorata ware according to claim 3 training culture medium, it is characterised in that the cinnamon be camphor tree, Cinnamomum kanahirai hay,
One or more in cinnamomum camphora, yellow camphor tree, great Ye camphor trees, heavy water camphor tree, Chinese cassia tree.
5. Antrodia camphorata ware according to claim 1 trains culture medium, it is characterised in that the Antrodia camphorata ware trains medium pH and is
4.5-5.5。
6. Antrodia camphorata ware according to claim 1 trains culture medium, it is characterised in that the Antrodia camphorata ware training culture medium also adds
Enter 10-20 parts of crisp Ye Jinmao moss extract.
7. Antrodia camphorata ware according to claim 6 trains culture medium, it is characterised in that the system of the crisp Ye Jinmao moss extract
Preparation Method is：Crisp Ye Jinmao moss is crushed, plus the 4-6 times of deionized water of parts by weight, with the strainer filtering of 0.5mm, at room temperature
Gentle agitation mixes 2h, 6000g, 30min, removes supernatant, adjusts pH to 7.2 with hydrochloric acid, 121 DEG C of autoclaving 15min, freeze-drying
Cheng Fen.
8. Antrodia camphorata ware described in claim 1 trains the preparation method of culture medium, it is characterised in that carry out in accordance with the following steps：First
Cinnamon mixture is prepared, then each component dissolving is mixed, and adjusts pH to 4.5-5.5,121 DEG C of autoclaving 20min, after sterilizing
Culture medium be down flat plate in superclean bench, per plate 20-30ml culture mediums, after being inoculated with Antrodia camphorata strain after culture medium solidifying.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108157065A (en) * | 2018-01-05 | 2018-06-15 | 江苏农林职业技术学院 | A kind of mycelial cultural method of Antrodia camphorata and its application |
| CN114568207A (en) * | 2022-03-11 | 2022-06-03 | 青岛浩然海洋科技有限公司 | Dish-type culture process for Antrodia camphorata |
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| CN104152359A (en) * | 2014-08-18 | 2014-11-19 | 中山安荞生物科技有限公司 | Taiwanofungus camphorates mycelium culturing medium and culturing method thereof |
| CN106212044A (en) * | 2016-07-27 | 2016-12-14 | 深圳市仁泰生物科技有限公司 | A kind of Antrodia Camphorata ware formula cultural method |
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| TWI231825B (en) * | 2000-09-05 | 2005-05-01 | Pei-Rung Li | Method for propagating fungi using solid state fermentation |
| CN102746054A (en) * | 2011-04-18 | 2012-10-24 | 甘泉生物科技有限公司 | Culture medium for culturing fruiting bodies of Antrodia cinnamomea and cultural method thereof |
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| CN114568207A (en) * | 2022-03-11 | 2022-06-03 | 青岛浩然海洋科技有限公司 | Dish-type culture process for Antrodia camphorata |
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