CN105347987A - Culture medium for substitute cultivation of ganoderma lucidum antler - Google Patents

Culture medium for substitute cultivation of ganoderma lucidum antler Download PDF

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Publication number
CN105347987A
CN105347987A CN201510982387.XA CN201510982387A CN105347987A CN 105347987 A CN105347987 A CN 105347987A CN 201510982387 A CN201510982387 A CN 201510982387A CN 105347987 A CN105347987 A CN 105347987A
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parts
obtains
mythic fungus
distiller
water
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CN201510982387.XA
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Chinese (zh)
Inventor
蒋晓艳
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Guilin Daye Lingyu Biological Technology Co Ltd
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Guilin Daye Lingyu Biological Technology Co Ltd
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Priority to CN201510982387.XA priority Critical patent/CN105347987A/en
Publication of CN105347987A publication Critical patent/CN105347987A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B17/00Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal

Abstract

The invention discloses a culture medium for substitute cultivation of ganoderma lucidum antler. The culture medium is prepared from raw materials in parts by weight as follows: 35-70 parts of wood flour, 25-60 parts of auxiliary materials, 0.5-1.5 parts of gypsum and 0.1-1 part of sugar, and the ratio of materials to water is controlled to be 1: (1.3-1.5) through a nutrient solution. The ratio of materials to water is controlled through the specially prepared nutrient solution, the prepared nutrient solution has high nutritional value, and the polysaccharide content is greatly increased when compared with that in the prior art. The nutrient solution, particularly used plant raw material ingredients, is subjected to steam explosion, distillation and treatment by a high shear homogenizer, nano milky and oily plant ingredients are obtained and have good compatibility with other formulas in the culture medium, and multiplication of multiple cells can be stimulated.

Description

A kind of Antler Mythic Fungus substituting stuff cultivation substratum
Technical field
The present invention relates to field of edible fungus culture, particularly relate to a kind of Antler Mythic Fungus substituting stuff cultivation substratum.
Background technology
Glossy ganoderma (formal name used at school: GanodermaLucidumKarst), also known as Ganoderma lucidum seu Japonicum, celestial grass, have another name called red sesame, red sesame, Ganoderma Lucidum, Ganoderma, 10000 years gill fungus, Ganoderma lucidum seu Japonicum, and being the complete stool of the red sesame of polyporaceae plant or purple sesame, is the sporophore of On Polyporaceae glossy ganoderma.Profile is umbrella, cap kidney shape, semicircle or subcircular.Glossy ganoderma, as the Chinese tradition valuable ingredient of traditional Chinese medicine having thousands of years medicinal histories, has invigorating QI and tranquilization, relieving cough and asthma effect, and for dizzy egersis, shortness of breath and palpitation, consumptive disease is coughed and breathed heavily.Glossy ganoderma possesses very high pharmaceutical use, and the modern pharmacology research through scientific research institution's many decades confirms, glossy ganoderma, for enhancing body immunity, regulates blood sugar, controls blood pressure, adjuvant therapy chemicotherapy, liver protecting, promotes that the aspects such as sleep all have significant curative effect.Ganoderan is one of principle active component of glossy ganoderma medicinal function; mainly be present in glossy ganoderma cell wall inwall; major part is beta-glucan; its monose composition is except containing except a large amount of glucose; also containing a small amount of pectinose, wood sugar, Fucose; rhamnosyl; semi-lactosi and seminose etc.; they are connected with glycosidic links such as (1-6) mainly with (1-3), (1-4) greatly; there is biological activity widely, as strengthening immunity, remove human free radical, protect cardiovascular, radioprotective, protection liver, the effect such as antitumor.Triterpenes is also one of effective constituent of glossy ganoderma, and triterpenes contained by glossy ganoderma not lower over one hundred kind, wherein based on tetracyclic triterpenes, the bitter taste of glossy ganoderma is relevant with contained triterpenes, to human hepatoma cell, there is cytotoxicity, also can suppress the release of histamine, have and protect the liver and the effects such as antianaphylaxis.
Antler Mythic Fungus is that likeness in form has the glossy ganoderma of a pair as deer horn body, it is undifferentiated cap in process of growth, be different from the pileus ganoderma lucidum that other are general, do not distribute spore powder, therefore do not consume maternal nutrition, nutrition is retained in sporophore completely, so the effective ingredient such as polysaccharide, ergosterol, N.F,USP MANNITOL, adenosine, triamino acid that Antler Mythic Fungus sporophore contains is higher than other common pileus ganoderma lucidum content, be the superfine product in glossy ganoderma, indices content is all considerably beyond sheet sesame.Such as, can improve the content of the organic germanium of human body cell resistance of oxidation up to 1680 to 2000ppm, be 3 to 4 times of sheet sesame, the contained macromolecule polysaccharide body that can strengthen immune function of human body is 43%, is more than 5 times of sheet sesame.Not only pharmaceutical use is high, and Antler Mythic Fungus and sheet sesame are carried out live body grafting, cultivates glossy ganoderma dish garden product innovation, more allows Antler Mythic Fungus have a sudden rise in social status.
Antler Mythic Fungus is adapted at growing containing in the matrix such as grape glucose, sucrose, starch, Mierocrystalline cellulose, xylogen.The agricultural byproducts such as general wood chip, cotton seed hulls and corn cob carry out production cultivation as substratum.Because region is different, by the impact of starting material factor, Antler Mythic Fungus is produced has certain limitation, is difficult to extensively carry out.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of Antler Mythic Fungus substituting stuff cultivation substratum of with low cost, successful is provided.
The present invention is achieved by the following technical solution:
A kind of Antler Mythic Fungus substituting stuff cultivation substratum, is made up of the raw material of following weight parts: wood chip 35-70 part, auxiliary material 25-60 part, gypsum 0.5-1.5 part, sugared 0.1-1 part, to control material-water ratio with nutritive medium be 1:1.3-1.5;
Described nutritive medium is made up of following part by weight composition:
PH is adjusted to be 6.0 with phosphate buffered saline buffer;
Described promotor adopts following methods preparation:
1) Feedstock treating: Herba Centellae is clean, drying, pulverizing;
2) Steam explosion treatment: by step 1) material that obtains puts into steam explosion tank, burstpressures 3.0-3.5Mpa, and the dwell time maintains 150-200s;
3) distillation process: by step 2) material that obtains puts into distiller, bottom distiller, heating flame or pass into steam, when hot steam is filled in distiller, water vapour, by prolong, is introduced in condenser, again by water-and-oil separator, collect essential oil;
4) high-shear homogenizing machine process: by step 3) essential oil that obtains is by high-shear homogenizing machine process, and linear velocity 30-40m/s, time 3-5min, makes nano-emulsion, and obtains with filtering with microporous membrane is degerming.
Preferably, be made up of the raw material of following weight parts: wood chip 42 parts, auxiliary material 53 parts, 1 part, gypsum, sugar 1 part, with nutritive medium control material-water ratio be 1:1.3-1.5.
Wood chip of the present invention preferably derives from maple and vertebra wood.
Described auxiliary material is one or more compositions in cotton seed hulls, wheat bran, Semen Maydis powder, when used as a mixture, can be arbitrary proportion.
A preparation method for Antler Mythic Fungus substituting stuff cultivation substratum, comprises the steps:
1) wood chip processing in early stage: pulverize after the trunk of trees after felling and branch peeling, dry for subsequent use;
2) batching, spice, windrow fermentation: prepare burden according to the formula of substratum, first wood chip, auxiliary material, gypsum, sugar mixing are formed siccative, then Ensure Liquid liquid fully mixes formation medium matrix thoroughly, after spice terminates, windrow ferments, nutritive medium is fully absorbed by medium matrix, can pack;
3) sterilizing: will install Bag Material bag and put into sterilizing device, high pressure steam sterilization or normal-pressure sterilization, moves into cooling room by material bag after sterilizing terminates, treats that temperature is down to less than 30 DEG C, can inoculate;
4) inoculate: by Antler Mythic Fungus strain inoculation in substratum, then postvaccinal bacterium bag is put into culturing room and cultivate, treat that mycelia covers with bacterium bag, proceed to fertility room after surperficial mycelia kink variable color and carry out sesame period management;
5) sesame period management is gone out: control room temp, humidity, CO 2concentration, intensity of illumination, pH value;
6) flower bud period management is dredged: in the young flower bud phase, manually remove small and weak sesame body, leave and take sturdy sesame body;
7) gather: gather in time when Antler Mythic Fungus is ripe.
Step 1 of the present invention) described in pulverizing, it is 0.5-1.0cm that preferred powder is broken to particle diameter.
Step 2) described in windrow fermentation, preferred control fermentation heap internal temperature is 65-70 DEG C, fermentation time is 1-2h, described pack, preferred 17X35X0.05 grade polypropylene plastics fruiting bag, feeds about 400 grams, charging should not be expired very much, material bag two tying, sack is noted cleaning, and prevents later stage living contaminants;
Step 3) described in high pressure steam sterilization, preferably 121 DEG C of sterilizing 1.5-2 hour, normal-pressure sterilization, preferably 100 DEG C of sterilizing 10-12 hour.
Step 4) described in inoculation, preferably according to the inoculum size of 1-3% (w/w), described culturing room require clean, clean, ventilate, between bacterium bag, temperature remains between 22-25 DEG C, relative air humidity remains on 55-60, cultivates, generally cultivate 30-40d under non-illuminated conditions.
Step 5) described in go out sesame period management, preferably control room temp 24-28 DEG C, humidity 75-85%, CO 2concentration 0.5-0.9, adopt fixed light source, intensity of illumination 300-4001x, pH value is 5.3-6.7.
Step 6) described in thin flower bud period management, preferably leave and take sturdy sesame body 1-2.
Compared with prior art, tool of the present invention has the following advantages:
1, in prior art, the substituting stuff cultivation substratum of preparation glossy ganoderma, control material-water ratio, generally adopt tap water or pure water, the present invention adopts special nutritive medium to control material-water ratio, being of high nutritive value of the Antler Mythic Fungus obtained, polysaccharide content significantly increases than prior art, and output increases by more than 15%.
2, the nutritive medium that the present invention is used, by adding trace metal ion and plant promoting agent, can stimulate the propagation of various kinds of cell.The plant material composition particularly used have passed through steam explosion, distillation, high-shear homogenizing machine process, obtains the plant oil components of nanometer emulsus, and in substratum, the consistency of other formulas is good.
Embodiment
With embodiment, the invention will be further described below, but the present invention is not limited to these embodiments.
Embodiment 1:
A kind of Antler Mythic Fungus substituting stuff cultivation substratum, is made up of following raw material: maple wood chip 35kg, cotton seed hulls 60kg, gypsum 0.5kg, sugared 0.1kg, to control material-water ratio with nutritive medium be 1:1.3;
Described nutritive medium is made up of following part by weight composition:
PH is adjusted to be 6.0 with phosphate buffered saline buffer;
Described promotor adopts following methods preparation:
1) Feedstock treating: Herba Centellae is clean, drying, pulverizing;
2) Steam explosion treatment: by step 1) material that obtains puts into steam explosion tank, burstpressures 3.0Mpa, and the dwell time maintains 200s;
3) distillation process: by step 2) material that obtains puts into distiller, bottom distiller, heating flame or pass into steam, when hot steam is filled in distiller, water vapour, by prolong, is introduced in condenser, again by water-and-oil separator, collect essential oil;
4) high-shear homogenizing machine process: by step 3) essential oil that obtains is by high-shear homogenizing machine process, and linear velocity 30m/s, time 5min, makes nano-emulsion, and obtains with filtering with microporous membrane is degerming.
Embodiment 2:
A kind of Antler Mythic Fungus substituting stuff cultivation substratum, is made up of following raw material: vertebra wood wood chip 70kg, cotton seed hulls 10kg, Semen Maydis powder 15kg, gypsum 1.5kg, sugared 1kg, to control material-water ratio with nutritive medium be 1:1.5;
Described nutritive medium is made up of following part by weight composition:
PH is adjusted to be 6.0 with phosphate buffered saline buffer;
Described promotor adopts following methods preparation:
1) Feedstock treating: Herba Centellae is clean, drying, pulverizing;
2) Steam explosion treatment: by step 1) material that obtains puts into steam explosion tank, burstpressures 3.5Mpa, and the dwell time maintains 180s;
3) distillation process: by step 2) material that obtains puts into distiller, bottom distiller, heating flame or pass into steam, when hot steam is filled in distiller, water vapour, by prolong, is introduced in condenser, again by water-and-oil separator, collect essential oil;
4) high-shear homogenizing machine process: by step 3) essential oil that obtains is by high-shear homogenizing machine process, and linear velocity 35m/s, time 3min, makes nano-emulsion, and obtains with filtering with microporous membrane is degerming.
Embodiment 3:
A kind of Antler Mythic Fungus substituting stuff cultivation substratum, is made up of following raw material: maple wood chip 42kg, wheat bran 53kg, gypsum 1kg, sugared 1kg, to control material-water ratio with nutritive medium be 1:1.4;
Described nutritive medium is made up of following part by weight composition:
PH is adjusted to be 6.0 with phosphate buffered saline buffer;
Described promotor adopts following methods preparation:
1) Feedstock treating: Herba Centellae is clean, drying, pulverizing;
2) Steam explosion treatment: by step 1) material that obtains puts into steam explosion tank, burstpressures 3.5Mpa, and the dwell time maintains 150s;
3) distillation process: by step 2) material that obtains puts into distiller, bottom distiller, heating flame or pass into steam, when hot steam is filled in distiller, water vapour, by prolong, is introduced in condenser, again by water-and-oil separator, collect essential oil;
4) high-shear homogenizing machine process: by step 3) essential oil that obtains is by high-shear homogenizing machine process, and linear velocity 40m/s, time 4min, makes nano-emulsion, and obtains with filtering with microporous membrane is degerming.
Comparative example 1:
A kind of Antler Mythic Fungus substituting stuff cultivation substratum, be made up of following raw material: willow wood chip 42kg, wheat bran 56kg, gypsum 1kg, sugared 1kg, material-water ratio is 1:1.4.
Comparative example 2:
A kind of Antler Mythic Fungus substituting stuff cultivation substratum, be made up of following raw material: maple wood chip 40kg, wheat bran 58kg, gypsum 0.5kg, sugared 1.5kg, fresh Herba Centellae 1kg, material-water ratio are 1:1.3.
Comparative example 3:
A kind of Antler Mythic Fungus substituting stuff cultivation substratum, is made up of following raw material: maple wood chip 40kg, wheat bran 58kg, gypsum 0.5kg, sugared 1.5kg, and the Herba Centellae residue 1kg after extraction activeconstituents, material-water ratio are 1:1.3.
The composition analysis of the Antler Mythic Fungus adopting embodiment and comparative example to obtain:
Result shows, the Antler Mythic Fungus nutritive ingredient that the embodiment of the present invention obtains is significantly higher than comparative example.

Claims (4)

1. an Antler Mythic Fungus substituting stuff cultivation substratum, is characterized in that, is made up of the raw material of following weight parts: wood chip 35-70 part, auxiliary material 25-60 part, gypsum 0.5-1.5 part, sugared 0.1-1 part, with nutritive medium control material-water ratio be 1:1.3-1.5;
Described nutritive medium is made up of following part by weight composition:
PH is adjusted to be 6.0 with phosphate buffered saline buffer;
Described promotor adopts following methods preparation:
1) Feedstock treating: Herba Centellae is clean, drying, pulverizing;
2) Steam explosion treatment: by step 1) material that obtains puts into steam explosion tank, burstpressures 3.0-3.5Mpa, and the dwell time maintains 150-200s;
3) distillation process: by step 2) material that obtains puts into distiller, bottom distiller, heating flame or pass into steam, when hot steam is filled in distiller, water vapour, by prolong, is introduced in condenser, again by water-and-oil separator, collect essential oil;
4) high-shear homogenizing machine process: by step 3) essential oil that obtains is by high-shear homogenizing machine process, and linear velocity 30-40m/s, time 3-5min, makes nano-emulsion, and obtains with filtering with microporous membrane is degerming.
2. a kind of Antler Mythic Fungus substituting stuff cultivation substratum according to claim 1, is characterized in that: be made up of the raw material of following weight parts: wood chip 42 parts, auxiliary material 53 parts, 1 part, gypsum, sugar 1 part, with nutritive medium control material-water ratio be 1:1.3-1.5.
3. a kind of Antler Mythic Fungus substituting stuff cultivation substratum according to claim 1 and 2, is characterized in that: described wood chip derives from maple and vertebra wood.
4. a kind of Antler Mythic Fungus substituting stuff cultivation substratum according to claim 1 and 2, is characterized in that: described auxiliary material is one or more compositions in cotton seed hulls, wheat bran, Semen Maydis powder, when used as a mixture, can be arbitrary proportion.
CN201510982387.XA 2015-12-24 2015-12-24 Culture medium for substitute cultivation of ganoderma lucidum antler Pending CN105347987A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107567959A (en) * 2017-09-29 2018-01-12 福建绿宝食品集团有限公司 A kind of production method of Ganoderma lucidum

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101066029A (en) * 2007-06-05 2007-11-07 浙江大学 Process of preparing colloidal edible fungus seed
CN103918475A (en) * 2014-03-26 2014-07-16 上海惠芬果蔬专业合作社 Pleurotus geesteranus potted culturing method and culture medium for culturing pleurotus geesteranus
CN104030845A (en) * 2014-06-30 2014-09-10 桂林丰茂源农业技术开发有限公司 Lucid ganoderma substitute cultivation medium with momordica grosvenori
CN104224716A (en) * 2014-10-14 2014-12-24 哈尔滨工业大学 Method for producing nanometer particles through nanometer emulsification technology
KR20140146789A (en) * 2013-06-18 2014-12-29 (주)한국농축산기술 System for total producing high speed and high quality amino acids fertilizers and feeds, using the slaughtered livestock's blood protein , and the producing method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101066029A (en) * 2007-06-05 2007-11-07 浙江大学 Process of preparing colloidal edible fungus seed
KR20140146789A (en) * 2013-06-18 2014-12-29 (주)한국농축산기술 System for total producing high speed and high quality amino acids fertilizers and feeds, using the slaughtered livestock's blood protein , and the producing method thereof
CN103918475A (en) * 2014-03-26 2014-07-16 上海惠芬果蔬专业合作社 Pleurotus geesteranus potted culturing method and culture medium for culturing pleurotus geesteranus
CN104030845A (en) * 2014-06-30 2014-09-10 桂林丰茂源农业技术开发有限公司 Lucid ganoderma substitute cultivation medium with momordica grosvenori
CN104224716A (en) * 2014-10-14 2014-12-24 哈尔滨工业大学 Method for producing nanometer particles through nanometer emulsification technology

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107567959A (en) * 2017-09-29 2018-01-12 福建绿宝食品集团有限公司 A kind of production method of Ganoderma lucidum

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Application publication date: 20160224