CN104030847A - Lucid ganoderma substitute cultivation medium with lupulus - Google Patents
Lucid ganoderma substitute cultivation medium with lupulus Download PDFInfo
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Abstract
The invention discloses a lucid ganoderma substitute cultivation medium with lupulus. The lucid ganoderma substitute cultivation medium comprises, by weight, 35-70 parts of sawdust, 25-60 parts of auxiliary material, 0.5-1.5 parts of gypsum, 0.1-1 part of sugar and 1-5 parts of lupulus, and the proportion of the materials to water is 1: 1.3-1.5. By means of the lucid ganoderma substitute cultivation medium, the growth of infectious microbe can be restrained very well in the cultivating process of lucid ganoderma, and the lucid ganoderma substitute cultivation medium has an obvious effect.
Description
Technical field
The present invention relates to field of edible fungus culture, relate in particular to a kind of glossy ganoderma substituting stuff cultivation substratum that has added hops.
Background technology
Glossy ganoderma (formal name used at school: Ganoderma Lucidum Karst), claim again Ganoderma lucidum seu Japonicum, celestial grass, have another name called red sesame, red sesame, Ganoderma Lucidum, Ganoderma, 10000 years gill fungus, Ganoderma lucidum seu Japonicum, be the complete stool of the red sesame of polyporaceae plant or purple sesame, is the sporophore of On Polyporaceae glossy ganoderma.Profile is umbrella, cap kidney shape, semicircle or subcircular.Glossy ganoderma, as the China's tradition valuable ingredient of traditional Chinese medicine that has thousands of years medicinal histories, has invigorating QI and tranquilization, relieving cough and asthma effect, and for dizzy egersis, shortness of breath and palpitation, consumptive disease is coughed and is breathed heavily.Glossy ganoderma possesses very high pharmaceutical use, studies confirm that through the modern pharmacology of scientific research institution's many decades, and glossy ganoderma, for strengthening body immunity, regulates blood sugar, controls blood pressure, auxiliary tumor chemoradiotherapy, and liver protecting, promotes the aspects such as sleep all to have significant curative effect.Ganoderan is one of main effective constituent of glossy ganoderma medicinal function; mainly be present in glossy ganoderma cell wall inwall; major part is beta-glucan; its monose forms except containing a large amount of glucose; also contain a small amount of pectinose, wood sugar, Fucose; rhamnosyl; semi-lactosi and seminose etc.; they are connected mainly with (1-3), (1-4) and glycosidic links such as (1-6) greatly; there is biological activity widely, as strengthening immunity, remove human free radical, protect cardiovascular, radioprotective, protection liver, the effect such as antitumor.Triterpenes is also one of effective constituent of glossy ganoderma, and not lower over one hundred kind of the contained triterpenes of glossy ganoderma, wherein taking tetracyclic triterpenes as main, the bitter taste of glossy ganoderma is relevant with contained triterpenes, human hepatoma cell is had to cytotoxicity, also can suppress the release of histamine, have and protect the liver and the effects such as antianaphylaxis.
Because the glossy ganoderma of self-sow is few, and contain a large amount of robust fibre and other impurity, and ganoderma polyoses content not high (being generally 2-5%) is not easy to directly as protective foods or pharmaceutical raw material, therefore, many scientific workers carry out artificial culture and the cultivation technique research of glossy ganoderma.At present, the technology such as material cultivation of artificial generation (bottle), fermentative production of glossy ganoderma obtains successfully in succession, and wherein, fermentative production is high to equipment requirements, and investment is large, is unfavorable for wide popularization and application; Ganoderma Lucidum is long-standing in China, and the development from cultivation basswood to substituting stuff cultivation technology has promoted glossy ganoderma artificial culture on a large scale.As Chinese patent CN103724115A, disclose a kind of glossy ganoderma bag and planted bacterium bag, the following raw materials by weight of main employing is formulated: mulberry wood chips 75-80%, cotton seed hulls 10-16%, wheat bran 3-5%, terra alba 0.8-1.2%, water content 55-65%, pH value 6.5-7.5, pack.Glossy ganoderma grow needed nutritive substance and other edible mushrooms unanimous on the whole on the whole, as carbon nutrition, nitrogen nutrition, VITAMIN and mineral substance etc., in the proportioning of glossy ganoderma substituting stuff cultivation culture medium, tradition major ingredient has cotton seed hulls, weed tree sawdust, agricultural crop straw etc., auxiliary material has wheat bran, wooden chaff, Semen Maydis powder etc., separately has the addition materials such as gypsum, calcium superphosphate, white sugar.Artificial substituting stuff cultivation is mainly made culturing raw material with the agricultural tankage such as wood chip, cotton seed hulls, bagasse, crop material and waste residue waste material, easy and simple to handle, less investment, be easy to generally apply, but waste residue waste material complicated component, what have may contain objectionable constituent, unfavorable to ganoderma lucidum prod quality control.Therefore finding novel substituting stuff cultivation substratum is the key of cultivation of glossy ganoderma industrialization.
Hops, another name: hops, lupulus, yeast flower, hops, Latin literary fame: Humulus lupulus is a kind of perennial herb trailing plant of Moraceae, Humulus, and ancients are taken as medicinal material.Hops begin for Germany, and formal name used at school is golden hop, dioecy, and zythepsary is with being female flower.The existing half a century of history of China's artificial culture hops, starts from northeast, covers the ground such as ﹑ Liaoning, the black imperial river of ﹑ and all set up larger hops raw material site in the sweet respectful ﹑ of the new boundary ﹑ of middle state.Ripe fresh hops drying squeezing, is used with whole hops, or packs after pulverizing compressed granulate, also can be made into hop extract, then in low temperature warehouse, preserves.Hops is widely used in brewing industry, and usage quantity is huge, and hops is also by the extraction for Flos lupuli (Flos Humuli Lupuli) extract in a large number, extract rear residue obtained as beer or hops, be all generally discarded, if the cultivation of energy and glossy ganoderma combines, there is huge economic worth.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of with low cost, successful interpolation the glossy ganoderma substituting stuff cultivation substratum of hops.
The present invention is achieved by the following technical solution:
Add a glossy ganoderma substituting stuff cultivation substratum for hops, made by the raw material of following weight parts: wood chip 35-70 part, auxiliary material 25-60 part, gypsum 0.5-1.5 part, sugared 0.1-1 part, hops 1-5 part, material-water ratio has been 1:1.3-1.5.
Preferably, made by the raw material of following weight parts: 42 parts of wood chips, 53 parts of auxiliary materials, 1 part, gypsum, 1 part of sugar, 3 parts of hops, material-water ratio is 1:1.3-1.5.
Described glossy ganoderma comprises preferred Antler Mythic Fungus, Ganoderma applanatum (pers) pat.
Described wood chip preferably originate maple and vertebra wood.
Described auxiliary material is one or more compositions in cotton seed hulls, wheat bran, Semen Maydis powder, in the time mixing use, can be arbitrary proportion.
Described hops is the residue obtaining after hops after drying, Hops Extract, production beer or the hops residue obtaining after extracting hops activeconstituents.
Described extraction hops activeconstituents technique comprises the techniques such as extraction, separation, purifying.
A preparation method who has added the glossy ganoderma substituting stuff cultivation substratum of hops, comprises the steps:
(1) wood chip processing in early stage: be 0.5-1.0cm by being crushed to particle diameter after the trunk of trees after felling and branch peeling, dry for subsequent use;
(2) batching: the formula according to substratum is prepared burden;
(3) spice: first wood chip, auxiliary material, gypsum, sugar, hops are mixed to formation siccative, then add water and fully mix formation medium matrix thoroughly, amount of water is held webs water breakthrough with hand grip but do not formed the globule, material-water ratio 1:1.3-1.5;
(4) pack: after spice finishes, windrow 1-2 hour, makes moisture fully be absorbed by medium matrix, can pack.
Preferably 17X35X0.05 specification acrylic plastering fruiting bag, feeds approximately 400 grams, and charging should not be expired very much, material bag two tying, and sack is noted cleaning, and prevents later stage living contaminants;
(5) sterilizing: material bag is put into sterilizing device, high pressure steam (121 DEG C) sterilizing 1.5-2 hour, or normal pressure (100 DEG C) sterilizing 10-12 hour, will expect after sterilizing finishes that bag moves into cooling room, treat that temperature is down to below 30 DEG C, can inoculate.
Compared with prior art, tool of the present invention has the following advantages:
1, hops, contains abundant fructose, sucrose, glucose etc., and the present invention adds hops at glossy ganoderma substituting stuff cultivation substratum, can utilize the carbohydrate, the protein etc. that in hops, contain, provides substratum abundant nutrient, reduces sugared interpolation.
2, hops, contain abundant resin, comprise humulone, isohumulone, lupulone etc., also contain a large amount of volatile oil, wherein there are myrcene, Humuleno, phantol, golden hop alcohol etc., particularly contained lupulone has restraining effect to tubercule bacillus, streptococcus aureus, Bacillus subtilus etc., can suppress the growth of gram positive bacterium, as Bacillus anthracis, bacillus cereus, diphtheria corynebacterium, pneumococcus, streptococcus aureus etc.The present invention, by adding hops at glossy ganoderma substituting stuff cultivation substratum, can give full play to the bacteria resistance function of hops, reduces substratum by the harm of living contaminants.
3, current, the beer production of China is in prostatitis, the world, the usage quantity of hops is huge, industrial as produce the residue obtaining after beer or the hop residue obtaining abandoned as waste after extracting hops activeconstituents, a general production technique is just for one or several composition in hops, and other composition useless making full use of, the present invention can make full use of the waste after hops is used, turn waste into wealth, increased the added value of hops.
Embodiment
With embodiment, the invention will be further described below, but the present invention is not limited to these embodiment.
Embodiment 1:
Add a Ganoderma applanatum (pers) pat substituting stuff cultivation substratum for hops, made by following raw material: the hops residue 5kg obtaining after maple wood chip 35kg, cotton seed hulls 60kg, gypsum 0.5kg, sugared 0.1kg, production beer, material-water ratio has been 1:1.3.
The preparation method of glossy ganoderma substituting stuff cultivation substratum, comprises the steps:
(1) wood chip processing in early stage: be 0.5-1.0cm by being crushed to particle diameter after the trunk of trees after felling and branch peeling, dry for subsequent use;
(2) batching: the formula according to substratum is prepared burden;
(3) spice: first wood chip, auxiliary material, gypsum, sugar, hops residue are mixed to formation siccative, then add water and fully mix formation medium matrix thoroughly, amount of water is held webs water breakthrough with hand grip but do not formed the globule;
(4) pack: after spice finishes, windrow 1-2 hour, makes moisture fully be absorbed by medium matrix, can pack.
Select 17X35X0.05 specification acrylic plastering fruiting bag, feed approximately 400 grams, charging should not be expired very much, material bag two tying, and sack is noted cleaning, and prevents later stage living contaminants;
(5) sterilizing: material bag is put into sterilizing device, and high pressure steam (121 DEG C) sterilizing 1.5-2 hour, will expect to wrap immigration cooling room after sterilizing finishes, treats that temperature is down to below 30 DEG C, can inoculate.
Embodiment 2:
A kind of Ganoderma applanatum (pers) pat substituting stuff cultivation substratum that has added hops, made by following raw material: vertebra wood wood chip 70kg, cotton seed hulls 10kg, Semen Maydis powder 15kg, gypsum 1.5kg, sugared 1kg, the hops residue 1kg obtaining after extracting hops activeconstituents, material-water ratio is 1:1.5.
The preparation method of glossy ganoderma substituting stuff cultivation substratum, comprises the steps:
(1) wood chip processing in early stage: be 0.5-1.0cm by being crushed to particle diameter after the trunk of trees after felling and branch peeling, dry for subsequent use;
(2) batching: the formula according to substratum is prepared burden;
(3) spice: first wood chip, auxiliary material, gypsum, sugar, hops residue are mixed to formation siccative, then add water and fully mix formation medium matrix thoroughly, amount of water is held webs water breakthrough with hand grip but do not formed the globule;
(4) pack: after spice finishes, windrow 1-2 hour, makes moisture fully be absorbed by medium matrix, can pack.
Select 17X35X0.05 specification acrylic plastering fruiting bag, feed approximately 400 grams, charging should not be expired very much, material bag two tying, and sack is noted cleaning, and prevents later stage living contaminants;
(5) sterilizing: material bag is put into sterilizing device, and normal pressure (100 DEG C) sterilizing 10-12 hour, will expect to wrap immigration cooling room after sterilizing finishes, treats that temperature is down to below 30 DEG C, can inoculate.
Embodiment 3:
Add an Antler Mythic Fungus substituting stuff cultivation substratum for hops, made by following raw material: maple wood chip 42kg, wheat bran 53kg, gypsum 1kg, sugared 1kg, Hops Extract 3kg, material-water ratio has been 1:1.4.
The preparation method of glossy ganoderma substituting stuff cultivation substratum, comprises the steps:
(1) wood chip processing in early stage: be 0.5-1.0cm by being crushed to particle diameter after the trunk of trees after felling and branch peeling, dry for subsequent use;
(2) batching: the formula according to substratum is prepared burden;
(3) spice: first wood chip, auxiliary material, gypsum, sugar, Hops Extract are mixed to formation siccative, then add water and fully mix formation medium matrix thoroughly, amount of water is held webs water breakthrough with hand grip but do not formed the globule;
(4) pack: after spice finishes, windrow 1-2 hour, makes moisture fully be absorbed by medium matrix, can pack.
Select 17X35X0.05 specification acrylic plastering fruiting bag, feed approximately 400 grams, charging should not be expired very much, material bag two tying, and sack is noted cleaning, and prevents later stage living contaminants;
(5) sterilizing: material bag is put into sterilizing device, and high pressure steam (121 DEG C) sterilizing 1.5-2 hour, will expect to wrap immigration cooling room after sterilizing finishes, treats that temperature is down to below 30 DEG C, can inoculate.
Embodiment 4:
Add an Antler Mythic Fungus substituting stuff cultivation substratum for hops, made by following raw material: vertebra wood wood chip 40kg, wheat bran 55kg, gypsum 1kg, sugared 0.8kg, hops 3.2kg after drying, material-water ratio has been 1:1.3.
The preparation method of glossy ganoderma substituting stuff cultivation substratum, comprises the steps:
(1) wood chip processing in early stage: be 0.5-1.0cm by being crushed to particle diameter after the trunk of trees after felling and branch peeling, dry for subsequent use;
(2) batching: the formula according to substratum is prepared burden;
(3) spice: first the hops by wood chip, auxiliary material, gypsum, sugar, after smashing mixes formation siccative, then adds water and fully mixes formation medium matrix thoroughly, and amount of water is held webs water breakthrough with hand grip but do not formed the globule;
(4) pack: after spice finishes, windrow 1-2 hour, makes moisture fully be absorbed by medium matrix, can pack.
Select 17X35X0.05 specification acrylic plastering fruiting bag, feed approximately 400 grams, charging should not be expired very much, material bag two tying, and sack is noted cleaning, and prevents later stage living contaminants;
(5) sterilizing: material bag is put into sterilizing device, and normal pressure (100 DEG C) sterilizing 10-12 hour, will expect to wrap immigration cooling room after sterilizing finishes, treats that temperature is down to below 30 DEG C, can inoculate.
Comparative example
A kind of Antler Mythic Fungus substituting stuff cultivation substratum, is made up of following raw material: willow wood chip 42kg, wheat bran 56kg, gypsum 1kg, sugared 1kg, material-water ratio is 1:1.4.
The preparation method of glossy ganoderma substituting stuff cultivation substratum, comprises the steps:
(1) wood chip processing in early stage: be 0.5-1.0cm by being crushed to particle diameter after the trunk of trees after felling and branch peeling, dry for subsequent use;
(2) batching: the formula according to substratum is prepared burden;
(3) spice: first wood chip, auxiliary material, gypsum, sugar are mixed to formation siccative, then add water and fully mix formation medium matrix thoroughly, amount of water is held webs water breakthrough with hand grip but do not formed the globule;
(4) pack: after spice finishes, windrow 1-2 hour, makes moisture fully be absorbed by medium matrix, can pack.
Select 17X35X0.05 specification acrylic plastering fruiting bag, feed approximately 400 grams, charging should not be expired very much, material bag two tying, and sack is noted cleaning, and prevents later stage living contaminants;
(5) sterilizing: material bag is put into sterilizing device, and high pressure steam (121 DEG C) sterilizing 1.5-2 hour, will expect to wrap immigration cooling room after sterilizing finishes, treats that temperature is down to below 30 DEG C, can inoculate.
By the glossy ganoderma substituting stuff cultivation substratum that embodiment and comparative example are obtained, under identical condition, carry out cultivation of glossy ganoderma experiment, and statistics is by the situation of living contaminants:
Table 1: living contaminants situation unit: %
By contrast, a kind of glossy ganoderma substituting stuff cultivation substratum that has added hops of the present invention can well suppress the growth of miscellaneous bacteria in the cultivation process of glossy ganoderma, has significant effect.
Table 2: mycelial growth situation
Substratum of the present invention is after hyphal development test, its mycelia rate of stretch is quick, mycelia stalwartness, and the later stage goes out in sesame test also to show that substratum of the present invention is for cultivating ganoderma, the cultivation cycle is moderate, and glossy ganoderma yield and quality is significantly improved with respect to prior art.The contained nutritive substance of substratum of the present invention can fully meet the basic demand of ganoderma lucidum growth and reproductive growth, and it is attractive in appearance that the Ganoderma sporophore cultivating through above-mentioned formula is very large, quality is superior, find with prior art cultivation contrast, the glossy ganoderma per unit area yield of substituting stuff cultivation substratum cultivation of the present invention exceeds more than 18%, and ganoderma polyoses content exceeds more than 16%.
Claims (6)
1. a glossy ganoderma substituting stuff cultivation substratum that has added hops, is characterized in that: the raw material by following weight parts is made: wood chip 35-70 part, auxiliary material 25-60 part, gypsum 0.5-1.5 part, sugared 0.1-1 part, hops 1-5 part, material-water ratio is 1:1.3-1.5.
2. a kind of glossy ganoderma substituting stuff cultivation substratum that has added hops according to claim 1, it is characterized in that: the raw material by following weight parts is made: 42 parts of wood chips, 53 parts of auxiliary materials, 1 part, gypsum, 1 part of sugar, 3 parts of hops, material-water ratio is 1:1.3-1.5.
3. a kind of glossy ganoderma substituting stuff cultivation substratum that has added hops according to claim 1 and 2, is characterized in that: described glossy ganoderma is Antler Mythic Fungus, Ganoderma applanatum (pers) pat.
4. a kind of glossy ganoderma substituting stuff cultivation substratum that has added hops according to claim 1 and 2, is characterized in that: described wood chip source maple and vertebra wood.
5. a kind of glossy ganoderma substituting stuff cultivation substratum that has added hops according to claim 1 and 2, is characterized in that: described auxiliary material is one or more compositions in cotton seed hulls, wheat bran, Semen Maydis powder, in the time mixing use, can be arbitrary proportion.
6. a kind of glossy ganoderma substituting stuff cultivation substratum that has added hops according to claim 1 and 2, is characterized in that: described hops is the residue obtaining after hops after drying, Hops Extract, production beer or the hops residue obtaining after extracting hops activeconstituents.
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| CN105418264A (en) * | 2015-12-24 | 2016-03-23 | 桂林大野领御生物科技有限公司 | Culture medium for substitute cultivation of ganoderma applanatum |
| CN105622256A (en) * | 2015-12-24 | 2016-06-01 | 桂林大野领御生物科技有限公司 | Cultivation method of ganoderma lucidum antler substitute material |
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| CN105622256A (en) * | 2015-12-24 | 2016-06-01 | 桂林大野领御生物科技有限公司 | Cultivation method of ganoderma lucidum antler substitute material |
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