CN104446710A - Lucid ganoderma cultivation medium, preparation method of lucid ganoderma cultivation medium and lucid ganoderma cultivation method - Google Patents
Lucid ganoderma cultivation medium, preparation method of lucid ganoderma cultivation medium and lucid ganoderma cultivation method Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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Abstract
The invention belongs to the field of agriculture and relates to a lucid ganoderma cultivation medium, a preparation method of the lucid ganoderma cultivation medium and a matched lucid ganoderma cultivation method. The invention aims at providing a lucid ganoderma cultivation medium which is wide in source and low in price and a preparation method of the lucid ganoderma cultivation medium. The lucid ganoderma cultivation medium disclosed by the invention comprises the following components in percentage by weight: 20-60 percent of apple branches, 20-50 percent of cottonseed hulls, 10-30 percent of corncobs and 10-15 percent of bran. The lucid ganoderma cultivation method comprises the following steps: (1) preparing materials; (2) mixing the materials; (3) bagging; (4) carrying out spawn running; and (5) discharging the lucid ganoderma to obtain ganoderma lucidum. The apple tree pruning branches are fully utilized, pollution-free treatment and recycling of the apple tree pruning branches are realized, a novel raw material is found for lucid ganoderma cultivation, and the cost of the lucid ganoderma is reduced. The byproducts of apple tree pruning branches are fully utilized, the cultivated lucid ganoderma is low in cost, the yield and the quality of the lucid ganoderma are superior to those of the conventional matrix or the effect is equivalent, and a novel cultivation raw material is provided for lucid ganoderma production.
Description
Technical field
The invention belongs to agriculture field, relate to a kind of cultivation of glossy ganoderma matrix and preparation method thereof and supporting ganoderma lucidum cultivation method.
Background technology
Glossy ganoderma has had nearly bimillennial medicinal history in China, is the legal Chinese medicinal materials of China, is also medicine-food two-purpose health product raw material.
Current cultivation of glossy ganoderma has cultivation basswood and cultivating in bag two kinds of modes.Cultivation basswood is the timber of the natural ecological woods utilizing non-aromatic smell, a kind of mode of cultivating ganoderma after simple process, its glossy ganoderma shape obtained is thicker, stem is longer, profile is received by the market, shortcoming is that biological transformation ratio is lower, has very large waste to timber resources, brings huge pressure to ecotope.Cultivating in bag is a kind of cultivation mode that development in recent years is got up, according to glossy ganoderma growth demand, by various matrix passing ratio mix and match, through a kind of mode of fruit pack-sterilizing-Fa bacterium-the go out techniqueflow production glossy ganoderma of sesame, it is the Main way of following cultivation of glossy ganoderma development, main raw material at present for Bag Material cultivation of glossy ganoderma is weed tree sawdust, cotton seed hulls, corn cob etc., but along with mushroom, Auricularia polytricha (Mout) Sacc., flat mushroom, the high speed development of the mushroom industries such as Pleurotus eryngii, cotton seed hulls, the general culturing raw material demand such as weed tree sawdust sharply increases, price rises year by year, cultivation of glossy ganoderma cost is caused to improve, the serious blow plantation enthusiasm of sesame agriculture.
In order to alleviate eco-environmental pressure, find the novel alternative materials of cultivation of glossy ganoderma, people have carried out glossy ganoderma matrix and planting type and have tried to explore.Patent publication No. CN102187782A describes a kind of method utilizing the Chinese medicinal materials such as the red sage root, chrysanthemum dis-medicinal part cultivating ganoderma; Patent publication No. CN103694016A describes a kind of matrix and the cultivating method thereof that utilize ramulus mori cultivating ganoderma; Patent publication No. CN 101317526A describes a kind of method utilizing tea branch, tealeaves, stalk generation material to produce glossy ganoderma; Patent publication No. CN103641615A describes a kind of method utilizing Sunflower Receptacle by product to make ganoderma lucidum cultivation material.Apple branch cultivating ganoderma matrix and supporting technology are not yet reported.The shortcoming of above-mentioned patent is: one is that the glossy ganoderma obtained by cultivations such as the red sage root, chrysanthemum, tea branches exists larger difference on mode of appearance with the glossy ganoderma of cultivation basswood, and the degree of recognition in market is lower; Two is that raw material sources have certain region limitation, quantitatively also has limitation, is unfavorable for large-scale promotion.
State Statistics Bureau's data show, apple is the first fruit of China, and 2003-2012 China apple production is totally in steady-state growth situation, and within 2012, China's apple production area is 223.13 ten thousand hm
2, account for 18.38% of China's gardens fruit cultivation total area.It is the inevitable operation of Apple Culture that fruit tree is pruned, and can produce about 1,000 ten thousand tons of pruning branches every year, burns or as offal treatment, resource is not utilized effectively and nuisanceless disposal usually used as firewood.
There is not yet the relevant report preparing cultivation of glossy ganoderma matrix with apple branch.
Summary of the invention
First technical problem solved by the invention is to provide a kind of wide material sources, cheap cultivation of glossy ganoderma matrix.
Cultivation of glossy ganoderma matrix of the present invention, calculates by mass percentage and comprises following component: apple branch 20%-60%, cotton seed hulls 20%-50%, corn cob 10%-30%, wheat bran 10%-15%.
Preferably, described cultivation matrix is calculated by mass percentage and is comprised following component: apple branch 40%-60%, cotton seed hulls 20%-30%, corn cob 10%-20%, wheat bran 10%-15%.
Most preferred, described cultivation matrix is calculated by mass percentage and is comprised following component: apple branch 50%, cotton seed hulls 20%, corn cob 15%, wheat bran 15%.
Second technical problem solved by the invention is to provide the preparation method of cultivation of glossy ganoderma matrix of the present invention, it is characterized in that: comprise the following steps:
(1) get the raw materials ready: apple branch is dry and be ground into particle;
(2) spice: after apple branch particle and cotton seed hulls, corn cob, wheat bran being weighed by formula rate, mixing tempers and makes cultivation matrix, and water content is 55-65%;
Wherein, described cultivation matrix is calculated by mass percentage and is comprised following component: apple branch 20%-60%, cotton seed hulls 20%-50%, corn cob 10%-30%, wheat bran 10%-15%;
Preferably, described cultivation matrix is calculated by mass percentage and is comprised following component: apple branch 40%-60%, cotton seed hulls 20%-30%, corn cob 10%-20%, wheat bran 10%-15%;
Most preferred, described cultivation matrix is calculated by mass percentage and is comprised following component: apple branch 50%, cotton seed hulls 20%, corn cob 15%, wheat bran 15%;
(3) pack: cultivation matrix packs, sterilizing, cooling, obtains cultivation of glossy ganoderma matrix.
In technique scheme, the drying described in step (1) refers to that it is 25-35% that apple branch is dried to water content.
In technique scheme, step (1) described drying is air-dry, oven dry.
In technique scheme, step (1) described pulverizing is for being crushed to granularity (0.5-1.2) cm × (0.4-0.8) cm × (0.1-0.3) cm.
3rd technical problem solved by the invention is to provide the ganoderma lucidum cultivation method of Ganoderma lucidum cultivation medium plasmogamy of the present invention cover, it is characterized in that, comprises the following steps:
(1) inoculate: the Ganoderma lucidum cultivation medium inoculation Ganderma lucidum strain described in any one of claim 1-3;
(2) send out bacterium: under appropriate conditions, mycelial growth 30-40 days starts sesame;
(3) sesame is gone out: obtain Ganoderma sporophore through going out to go out for 25-35 days sesame management.
In technique scheme, the inoculation described in step (1) is two inoculation, and with nonwoven cloth material sealing, inoculum size is the 1-2% of matrix weight.
In technique scheme, described in step (2) suitable condition temperature be 22-26 DEG C, relative air humidity is 45-60%.
In technique scheme, described in step (3) go out sesame management temperature be 25-30 DEG C, relative air humidity is 85-95%, and intensity of illumination is 4000-6000Lx.
The present invention utilizes apple branch Substitute For Partial conventional substrate cultivating ganoderma to have the following advantages:
One is that apple branch matrix and cultivation technique can effectively reduce glossy ganoderma production cost.Apple is first fruit, apple pruning branch is wide compared with sources such as the red sage root mentioned in background technology, chrysanthemum, tea branch, Sunflower Receptacle by products, quantity is large, comparatively the traditional raw material such as cotton seed hulls, corn cob price is low, can effectively reduce glossy ganoderma production cost, production cost as traditional glossy ganoderma is 2-2.5 unit/bag, the production cost of apple branch is 1.5-1.7 unit/bag, and the present invention every mu can reduce costs 1500-2400 unit; In addition, apple branch comminution process is easy to operate compared with the red sage root, chrysanthemum, tea branch, Sunflower Receptacle by product etc., has good operability and generalization.
Two is that the glossy ganoderma economical character that apple branch substrate culture obtains is good.Apple branch particle is compared with the red sage root mentioned in background technology, chrysanthemum, tea branch, the Material Physics structure of Sunflower Receptacle by product is more fine and close, carbon-nitrogen ratio is 300-500:1, content of lignin is relatively high, its physical structure and chemical composition are more close to natural linden, cap thickness at least more than the 9mm of the glossy ganoderma that cultivation obtains, more traditional Bag Material glossy ganoderma increases 1-2mm, Ganoderma sporophore more than substance 90g, more traditional Bag Material glossy ganoderma increases 5-6g, a glossy ganoderma shape is thicker, stem is longer, form is more close to linden glossy ganoderma, product is by the welcome in market, market price is 2-3 times of common Bag Material glossy ganoderma.
Three is pollution rates that apple branch matrix effectively can reduce glossy ganoderma production process.The natural ph of apple branch is 7.5-8, culture medium does not need to add the acid-alkali accommodation such as lime, gypsum material in addition, the characteristic of self meta-alkalescence can improve matrix antifungal pollution capacity, test shows that the glossy ganoderma of apple branch substrate culture is under same high pollution environment, pollution rate is below 4.5%, and comparatively conventional substrate reduces 2-4%.And the height of pollution rate is the key factor affecting benefit in glossy ganoderma production, the per mu yield value that the present invention increases because reducing pollution rate is 5000 yuan.
Four is that ganoderma lucidum cultivation method of the present invention adopts non-woven fabrics sealing effectively to reduce the pollution rate of Neurospora.Non-woven fabrics has the advantages such as ventilative, antibacterial, pest-resistant, corrosion-resistant, price is low, overcome Normal practice and adopt plastic film sealing poor air permeability, newspaper is adopted to seal the shortcoming of easy bacteria infection, effectively reduce the most liable to infection Neurospora contamination probability of Bag Material glossy ganoderma in summer, improve glossy ganoderma surviving rate, adopt non-woven fabrics Sealling Technique per mu yield value can improve 1000 yuan.
To sum up, apple branch of the present invention is the by product that the annual pruning of apple tree obtains, source and extensively, cheap, usually used as offal treatment; The present invention adopts apple pruning branch to prepare culture medium, pollution rate is obviously lower, the glossy ganoderma cost cultivated out is low, yield and quality is suitable with conventional substrate effect, even more excellent, the Medium for Ganoderma lucidum matter that the present invention is prepared with apple branch and cultivation method for matching be a kind of brand-new selection mode for nuisanceless disposal provides.
Embodiment
Embodiment 1---compare the difference adopting non-woven fabrics sealing and conventional newspaper/plastic film Sealling Technique glossy ganoderma pollution rate
1, cultivation of glossy ganoderma matrix: calculate by mass percentage and comprise following component: apple branch 30%, cotton seed hulls 40%, corn cob 15%, wheat bran 15%.、
2, the preparation of culture medium:
(1) get the raw materials ready: by air-dry for each fruit tree and be ground into particle, branch is air-dry is 33% of apple branch dry weight to its water content, and the granular size specification of pulverizing is: (0.5-1.2) cm × (0.4-0.8) cm × (0.1-0.3) cm;
(2) spice: mixed with unclassified stores by formula by each fruit tree particle, temper, water content is 60%;
(3) pack: cultivation matrix prepares mixes laggard luggage bag thoroughly, and the specification of sack is the polyethylene plastic bag of 22mm × 43mm × 0.25mm, after charging, the weight of sack is 2.3kg, then carries out sterilising treatment, inoculates Ganderma lucidum strain after cooling; Under more different cultivating method, the difference of glossy ganoderma pollution rate:
3, cultivation condition:
(1) inoculate:
Method one is after cover circle suitable for reading, and with the nonwoven cloth material sealing that 8cm is square, inoculum size is 1% of matrix weight;
Method two is after cover circle suitable for reading, and with the newspaper sealing that 8cm is square, inoculum size is 1% of matrix weight;
Method three is after cover circle suitable for reading, and with the plastic film sealing that 8cm is square, inoculum size is 1% of matrix weight;
(2) send out bacterium: temperature 25 DEG C, relative air humidity is that 55% condition issues bacterium.
(3) go out sesame: be 28 DEG C in temperature, relative air humidity is 90%, and under illumination 3000lx condition, management obtains Ganoderma sporophore.
Table 2 test-results compares
Test-results shows, comparatively other two kinds of Sealling Techniques, non-woven fabrics Sealling Technique of the present invention can reduce the pollution rate of more than 2%, improve the success ratio of cultivation of glossy ganoderma, calculate according to every mu of 3000 bags of glossy ganodermas, two tides of gathering for a year, adopt this programme per mu yield can increase by 11 kilograms, the output value improves 1100 yuan.
Embodiment 2---compare the economical character and glossy ganoderma quality that utilize different cultivation matrix cultivating ganoderma
1, culture medium
Cultivation of glossy ganoderma matrix (1): calculate by mass percentage and comprise following component: apple branch 50%, cotton seed hulls 20%, corn cob 15%, wheat bran 15%.
Cultivation of glossy ganoderma matrix (2): calculate by mass percentage and comprise following component: apple branch 30%, cotton seed hulls 40%, corn cob 15%, wheat bran 15%.
Above-mentioned formula and following formula are contrasted:
Pear tree branch cultivation matrix (contrast 1-1): calculate by mass percentage and comprise following component: pear tree branch 50%, cotton seed hulls 20%, corn cob 15%, wheat bran 13%, gypsum 2%.
Pear tree branch cultivation matrix (contrast 1-2): calculate by mass percentage and comprise following component: pear tree branch 30%, cotton seed hulls 40%, corn cob 15%, wheat bran 13%, gypsum 2%.
Peach branch cultivation matrix (contrast 2-1): calculate by mass percentage and comprise following component: peach branch 50%, cotton seed hulls 20%, corn cob 15%, wheat bran 13%, gypsum 2%.
Peach branch cultivation matrix (contrast 2-2): calculate by mass percentage and comprise following component: peach branch 30%, cotton seed hulls 40%, corn cob 15%, wheat bran 13%, gypsum 2%.
Add the culture medium (3-1) of gypsum: apple branch 50%, cotton seed hulls 20%, corn cob 15%, wheat bran 13%, gypsum 2%.
Add the culture medium (3-2) of gypsum: apple branch 30%, cotton seed hulls 40%, corn cob 15%, wheat bran 13%, gypsum 2%.
Conventional cultivation matrix (contrast 4-1): calculate by mass percentage and comprise following component: cotton seed hulls 70%, corn cob 15%, wheat bran 13%, gypsum 2%.
Conventional cultivation matrix (contrast 4-2): calculate by mass percentage and comprise following component: cotton seed hulls 50%, corn cob 35%, wheat bran 13%, gypsum 2%.
2, the preparation of culture medium:
(1) get the raw materials ready: by air-dry for each fruit tree and be ground into particle, branch is air-dry is 33% of apple branch dry weight to its water content, and the granular size specification of pulverizing is: (0.5-1.2) cm × (0.4-0.8) cm × (0.1-0.3) cm;
(2) spice: mixed with unclassified stores by formula by each fruit tree particle, temper, water content is 60%;
(3) pack: cultivation matrix prepares mixes laggard luggage bag thoroughly, and the specification of sack is the polyethylene plastic bag of 22mm × 43mm × 0.25mm, after charging, the weight of sack is 2.3kg, then carries out sterilising treatment, inoculates Ganderma lucidum strain after cooling; Vaccination ways is two inoculation, and with the nonwoven cloth material sealing that 8cm is square, inoculum size is 1% of matrix weight.
3, cultivation condition:
(1) send out bacterium: temperature 25 DEG C, relative air humidity is that 55% condition issues bacterium.
(2) go out sesame: be 28 DEG C in temperature, relative air humidity is 90%, and under illumination 3000lx condition, management obtains Ganoderma sporophore.
Results contrast
Table 1 difference cultivation formula glossy ganoderma growth indexes situation compares
Test-results shows, and formula of the present invention is the best formula of glossy ganoderma profile in above-mentioned experimental control formula, growing way, output.Compare with cellar culture matrix, adopt the glossy ganoderma purseful of the present invention's cultivation and go out sesame time shorten 2-3 days, pollution rate reduces 2.6-2.8%, and a glossy ganoderma shape hypertrophy is mellow and full, and sporophore thickness increases 1.3-2.1mm, and single sporophore weight increases 6.1-8.1g.With add the recipe ratio of gypsum comparatively, fill a prescription glossy ganoderma profile and economical character difference of the present invention is remarkable, illustrates that formula of the present invention glossy ganoderma proterties when not adding gypsum is excellent.From per mu yield and Economic and Efficiency Analysis, according to every mu of 3000 bags of glossy ganodermas, the mode of two tides of gathering for a year calculates, and the present invention every mu glossy ganoderma output can reach 600 kilograms, and per mu yield comparatively conventional substrate improves 10%, and per mu yield value can increase by 5000 yuan.
In addition, because the natural ph of apple branch is 7.5-8, prepare culture medium with apple branch, adding the acid-alkali accommodation such as gypsum, lime material does not affect.Simultaneously because the characteristic of himself meta-alkalescence can improve matrix antifungal pollution capacity, above-mentioned test shows that the glossy ganoderma of apple branch substrate culture is under same high pollution environment, and pollution rate is below 4.5%, and comparatively conventional substrate reduces 2-4%.
To sum up, the present invention adopts apple pruning branch to prepare culture medium, pollution rate is obviously lower, the glossy ganoderma cost cultivated out is low, yield and quality is suitable with conventional substrate effect, even more excellent, the Medium for Ganoderma lucidum matter that the present invention is prepared with apple branch and cultivation method for matching be a kind of brand-new selection mode for nuisanceless disposal provides.
Claims (10)
1. cultivation of glossy ganoderma matrix, is characterized in that: described cultivation matrix is calculated by mass percentage and comprised following component: apple branch 20%-60%, cotton seed hulls 20%-50%, corn cob 10%-30%, wheat bran 10%-15%.
2. cultivation of glossy ganoderma matrix according to claim 1, is characterized in that: described cultivation matrix is calculated by mass percentage and comprised following component: apple branch 40%-60%, cotton seed hulls 20%-30%, corn cob 10%-20%, wheat bran 10%-15%.
3. cultivation of glossy ganoderma matrix according to claim 1, is characterized in that: described cultivation matrix is calculated by mass percentage and comprised following component: apple branch 50%, cotton seed hulls 20%, corn cob 15%, wheat bran 15%.
4. the preparation method of cultivation of glossy ganoderma matrix, is characterized in that: comprise the following steps:
(1) get the raw materials ready: apple branch is dry and be ground into particle;
(2) spice: after apple branch particle and cotton seed hulls, corn cob, wheat bran being weighed by formula rate, mixing tempers and makes cultivation matrix, and water content is 55-65%;
Wherein, described cultivation matrix is calculated by mass percentage and is comprised following component: apple branch 20%-60%, cotton seed hulls 20%-50%, corn cob 10%-30%, wheat bran 10%-15%;
(3) pack: cultivation matrix packs, sterilizing, cooling, obtains cultivation of glossy ganoderma matrix.
5. the preparation method of cultivation of glossy ganoderma matrix according to claim 4, it is characterized in that: described cultivation matrix is calculated by mass percentage and comprised following component: apple branch 40%-60%, cotton seed hulls 20%-30%, corn cob 10%-20%, wheat bran 10%-15%;
Preferably, described cultivation matrix is calculated by mass percentage and is comprised following component: apple branch 50%, cotton seed hulls 20%, corn cob 15%, wheat bran 15%.
6. the preparation method of cultivation of glossy ganoderma matrix according to claim 4, is characterized in that: the drying described in step (1) refers to that it is 25-35% that apple branch is dried to water content;
Preferably, described drying is air-dry, oven dry;
Preferably, described pulverizing is for being crushed to granularity (0.5-1.2) cm × (0.4-0.8) cm × (0.1-0.3) cm.
7. the cultivating method of glossy ganoderma, is characterized in that: comprise the following steps:
(1) inoculate: the Ganoderma lucidum cultivation medium inoculation Ganderma lucidum strain described in any one of claim 1-3;
(2) send out bacterium: under appropriate conditions, mycelial growth 30-40 days starts sesame;
(3) sesame is gone out: obtain Ganoderma sporophore through going out to go out for 25-35 days sesame management.
8. ganoderma lucidum cultivation method according to claim 7, is characterized in that: the inoculation described in step (1) is two inoculation, and with nonwoven cloth material sealing, inoculum size is the 1-2% of matrix weight.
9. ganoderma lucidum cultivation method according to claim 7, is characterized in that: described in step (2) suitable condition temperature be 22-26 DEG C, relative air humidity is 45-60%.
10. ganoderma lucidum cultivation method according to claim 7, is characterized in that: described in step (3) go out sesame management temperature be 25-30 DEG C, relative air humidity is 85-95%, and intensity of illumination is 4000-6000Lx.
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| CN105272569A (en) * | 2015-10-15 | 2016-01-27 | 田野 | Ganoderma-lucidum culture medium and ganoderma lucidum plantation method |
| CN105265178A (en) * | 2015-11-20 | 2016-01-27 | 四川省农业科学院 | Lucid ganoderma indoor continuous cropping cut log cultivation method |
| CN105646067A (en) * | 2015-12-30 | 2016-06-08 | 桂林大野领御生物科技有限公司 | Lucid ganoderma cultivation method for promoting mycelial growth |
| CN106954459A (en) * | 2017-03-22 | 2017-07-18 | 宁夏田媛菌草有限公司 | A kind of implantation methods of ganoderma lucidum |
| CN108864319A (en) * | 2018-06-29 | 2018-11-23 | 江苏农林职业技术学院 | The method and matrix used of ganoderma lucidum solid state fermentation production active polysaccharide |
| CN109220552A (en) * | 2018-11-16 | 2019-01-18 | 四川岚晟生物科技有限公司 | Cultivation of glossy ganoderma matrix and preparation method, ganoderma lucidum cultivation method based on walnut shell |
| CN109315221A (en) * | 2018-11-16 | 2019-02-12 | 四川岚晟生物科技有限公司 | Cultivation of glossy ganoderma matrix based on ganoderma lucidum slag and preparation method thereof, ganoderma lucidum cultivation method |
| CN110089352A (en) * | 2019-06-10 | 2019-08-06 | 安徽金寨仙芝灵生物科技有限公司 | A kind of lucidum spore powder utilizes the collection method of non-woven fabrics |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105272569A (en) * | 2015-10-15 | 2016-01-27 | 田野 | Ganoderma-lucidum culture medium and ganoderma lucidum plantation method |
| CN105265178A (en) * | 2015-11-20 | 2016-01-27 | 四川省农业科学院 | Lucid ganoderma indoor continuous cropping cut log cultivation method |
| CN105646067A (en) * | 2015-12-30 | 2016-06-08 | 桂林大野领御生物科技有限公司 | Lucid ganoderma cultivation method for promoting mycelial growth |
| CN106954459A (en) * | 2017-03-22 | 2017-07-18 | 宁夏田媛菌草有限公司 | A kind of implantation methods of ganoderma lucidum |
| CN108864319A (en) * | 2018-06-29 | 2018-11-23 | 江苏农林职业技术学院 | The method and matrix used of ganoderma lucidum solid state fermentation production active polysaccharide |
| CN109220552A (en) * | 2018-11-16 | 2019-01-18 | 四川岚晟生物科技有限公司 | Cultivation of glossy ganoderma matrix and preparation method, ganoderma lucidum cultivation method based on walnut shell |
| CN109315221A (en) * | 2018-11-16 | 2019-02-12 | 四川岚晟生物科技有限公司 | Cultivation of glossy ganoderma matrix based on ganoderma lucidum slag and preparation method thereof, ganoderma lucidum cultivation method |
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Application publication date: 20150325 |