CN109220552A - Cultivation of glossy ganoderma matrix and preparation method, ganoderma lucidum cultivation method based on walnut shell - Google Patents
Cultivation of glossy ganoderma matrix and preparation method, ganoderma lucidum cultivation method based on walnut shell Download PDFInfo
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- CN109220552A CN109220552A CN201811367155.3A CN201811367155A CN109220552A CN 109220552 A CN109220552 A CN 109220552A CN 201811367155 A CN201811367155 A CN 201811367155A CN 109220552 A CN109220552 A CN 109220552A
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- walnut shell
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Coloring Foods And Improving Nutritive Qualities (AREA)
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Abstract
The invention discloses a kind of cultivation of glossy ganoderma matrix and preparation method, ganoderma lucidum cultivation method based on walnut shell are crushed by raw material, remove Hydrolysable Tannins in walnut shell, the condensed tannin in removal walnut shell, finally obtained through screening and optimum organization using walnut shell as the matrix of raw material cultivating ganoderma.Matrix formulations are as follows: walnut shell particle accounting 40~50%, cotton seed hulls particle accounting 25~30%, corn flour accounting 20~25%, wheat bran accounting 5~10%, land plaster 1~1.5%.Ganoderma lucidum cultivation method based on above-mentioned cultivation of glossy ganoderma matrix, by the cultivation of glossy ganoderma matrix injected clear water and stir, moisture control is in 60~65% packs, 0.1~0.15MPa after pack, 121 DEG C of 30~45min of sterilizing, let cool to room temperature, breeding are carried out in 20-30 DEG C of incubator, routine observation mycelia upgrowth situation, until growing ganoderma lucidum fruitbody.The present invention reaches the bacteriostasis for thoroughly destroying tannin, while the bean cake powder added can also be used as the source of nutrition of Medium for Ganoderma lucidum matter, be conducive to the growth of ganoderma lucidum.
Description
Technical field
The present invention relates to cultivation of glossy ganoderma field, especially a kind of cultivation of glossy ganoderma matrix and preparation method based on walnut shell,
Ganoderma lucidum cultivation method.
Background technique
The ganoderma lucidum artificial cultivation in China years development, have cultivating in bag also as culture substrate and have it is segment wood cultivated,
In especially with segment wood cultivated ganoderma lucidum closest to natural growthing condition, be considered best in quality, in recent years due to the deflation of forest resourceies,
Wood cultured Ganoderma lucidum is badly in need of alternative.
The ground such as Sichuan possess the ecotopia of cultivation of glossy ganoderma, while also abounding with walnut, strip the walnut discarded after walnut kernel
Shell is because constituting the cultivation close to timber, with the high-quality ganoderma lucidum of tradition containing a large amount of celluloses, hemicellulose, these main components of lignin
Matrix-log is similar, can be decomposed and absorb by ganoderma lucidum mycelium, is suitble to the ganoderma lucidum growth of the raw mushroom of wood, is ideal cultivation base
Matter, but due to containing tannin in walnut shell, tannin has stronger bacteriostasis, has centainly to the growth of normal ganoderma lucidum mycelium
Inhibiting effect, therefore walnut shell cannot efficiently use for a long time.
Tannin is the polyhydric phenols that a class formation is more complicated in walnut shell, can be divided into hydrolysis by chemical structure
Tannin and condensed tannin, they have similar biological characteristics, and it includes edible to ganoderma lucidum etc. for having inhibiting effect to microorganism
The inhibiting effect of bacterium mycelial growth.Hydrolysable Tannins is water-soluble, and condensed tannin is then not soluble in water, and walnut shell is directly soaked
Bubble, can remove partial hydrolysis tannin, but not can be removed condensed tannin, and condensed tannin then continues to inhibit ganoderma lucidum to a certain extent
The growth of equal edible mushroom myceliums.If long-time is impregnated, the nutriment (such as polysaccharide, protein) in walnut shell is gradually molten
Xie Yushui causes the loss of nutriment, is unfavorable for the growth of the edible mushroom myceliums such as ganoderma lucidum.
Summary of the invention
The cultivation of glossy ganoderma matrix and preparation method that technical problem to be solved by the invention is to provide a kind of based on walnut shell,
Ganoderma lucidum cultivation method generates the compound organic matter for being insoluble in water, using tannin in conjunction with protein to remove the list in walnut shell
Rather, walnut shell particle after processing is configured to according to a certain percentage with cotton seed hulls particle, corn particle, wheat bran, land plaster
Cultivation of glossy ganoderma matrix, pack, sterilizing, inoculation lucidum strain turn out sesame.
In order to solve the above technical problems, the technical solution adopted by the present invention is that:
A kind of preparation method of the cultivation of glossy ganoderma matrix based on walnut shell, comprising the following steps:
Step 1: raw material crushes
Dry walnut shell is crushed into the particle for 3-5mm, cotton seed hulls is ground into the particle of 1-2mm, by crush maize at 1-
Dregs of beans is ground into the particle of 1-2mm by the particle of 2mm；
Step 2: Hydrolysable Tannins in removal walnut shell
Smashed walnut shell is put into fermentation vat, 5~10 times of walnut shell particle dry weight of water, first time room temperature is added
After impregnating 2~4h, soak is discarded；
It is added 5~8 times of walnut shell particle dry weight of water, after second of 2~4h of soak at room temperature, discards soak；
Step 3: the condensed tannin in removal walnut shell
5~8 times of walnut shell particle dry weight of water is added, adds the bean cake powder of walnut shell particle dry weight 10%~20%,
Combination is sufficiently stirred, is uniformly mixed it, after 48~72h of room temperature, pulls the walnut shell particle on upper layer out, be filtered dry moisture；
Step 4: culture medium is prepared
The cultivation of glossy ganoderma matrix of preparation includes cotton seed hulls particle through step 1 preparation and corn particle, handles through step 3
Walnut shell particle and wheat bran, land plaster afterwards.
Further, in step 2, after 8 times of walnut shell particle dry weight of water immersion for the first time is first added, core is added
Second of immersion of the water that 6 times of dry weight of peach shell particle.
Further, in step 3, the bean cake powder placement processing of walnut shell particle dry weight 15% is added.
According to cultivation of glossy ganoderma matrix prepared by above-mentioned preparation method, composition are as follows: walnut shell particle accounting 40~50%, cottonseed
Shell particle accounting 25~30%, corn particle accounting 20~25%, wheat bran accounting 5~10%, land plaster 1~1.5%.
Ganoderma lucidum cultivation method based on above-mentioned cultivation of glossy ganoderma matrix, comprising the following steps: infuse the cultivation of glossy ganoderma matrix
Entering clear water and stirs, moisture control is packed 60~65%, 0.1~0.15MPa after pack, 121 DEG C of sterilizings 30~
45min is let cool to room temperature, after being inoculated with lucidum strain, breeding is carried out in 20-30 DEG C of incubator, routine observation mycelia grows shape
Condition, until growing ganoderma lucidum fruitbody.
Compared with prior art, the beneficial effects of the present invention are: first going to remove water 1) by 2 water logging juglans sigillata shell particles
After solving tannin, the compound organic matter characteristic for being insoluble in water is generated in conjunction with protein using condensed tannin, condensed tannin is removed, reaches
To the thorough bacteriostasis for destroying tannin, while the bean cake powder added can also be used as the source of nutrition of Medium for Ganoderma lucidum matter, more
Be conducive to the growth of ganoderma lucidum.
Specific embodiment
Below by specific embodiment, the present invention is described in further detail.
Embodiment 1
It the preparation method of cultivation of glossy ganoderma matrix, the cultivation of glossy ganoderma matrix being prepared and is carried out with this matrix in the present invention
Cultivation of glossy ganoderma, details are as follows:
One, raw material crushes
Dry walnut shell is crushed as the particle of diameter 3mm, cotton seed hulls is ground into the particle of 2mm, by crush maize at 1mm
Particle, dregs of beans is ground into the particle of 2mm.
Two, Hydrolysable Tannins in walnut shell is removed
Smashed walnut shell is put into fermentation vat, 10 times of walnut shell particle dry weight of water, the leaching of first time room temperature is added
After steeping 4h, soak is discarded；
It is added 8 times of walnut shell particle dry weight of water, after second of soak at room temperature 4h, discards soak；
Three, the condensed tannin in walnut shell is removed
8 times of walnut shell particle dry weight of water is added, adds the bean cake powder of walnut shell particle dry weight 10%, knot is sufficiently stirred
It closes, is uniformly mixed it, after room temperature 72h, pulls the walnut shell particle on upper layer out, be filtered dry moisture；
Four, culture medium is prepared
It finally establishes through screening and optimum organization using walnut shell as the matrix formulations of raw material cultivating ganoderma: walnut shell particle
45%, cotton seed hulls particle 25%, corn flour 20%, wheat bran 9%, land plaster 1%.
Five, breeding cultivation
It by the cultivation of glossy ganoderma matrix injected clear water and stirs, moisture control is in 60% pack, 0.1 after pack
~0.15MPa, 121 DEG C of sterilizing 30min let cool to room temperature, after being inoculated with lucidum strain, carry out breeding in 30 DEG C of incubators, fixed
Phase observes mycelia upgrowth situation, until growing ganoderma lucidum fruitbody.
Embodiment 2
It the preparation method of cultivation of glossy ganoderma matrix, the cultivation of glossy ganoderma matrix being prepared and is carried out with this matrix in the present invention
Cultivation of glossy ganoderma, details are as follows:
One, raw material crushes
Dry walnut shell is crushed as the particle of diameter 3mm, cotton seed hulls is ground into the particle of 1mm, by crush maize at 2mm
Particle, dregs of beans is ground into the particle of 1mm.
Two, Hydrolysable Tannins in walnut shell is removed
Smashed walnut shell is put into fermentation vat, 8 times of walnut shell particle dry weight of water, first time soak at room temperature is added
After 3, soak is discarded；
It is added 6 times of walnut shell particle dry weight of water, after second of soak at room temperature 3h, discards soak；
Three, the condensed tannin in walnut shell is removed
6 times of walnut shell particle dry weight of water is added, adds the bean cake powder of walnut shell particle dry weight 15%, knot is sufficiently stirred
It closes, is uniformly mixed it, after room temperature 60h, pulls the walnut shell particle on upper layer out, be filtered dry moisture；
Four, culture medium is prepared
It finally establishes through screening and optimum organization using walnut shell as the matrix formulations of raw material cultivating ganoderma: walnut shell particle
49%, cotton seed hulls particle 25%, corn flour 20%, wheat bran 5%, land plaster 1%.
Five, breeding cultivation
It by the cultivation of glossy ganoderma matrix injected clear water and stirs, moisture control is in 62% pack, 0.1 after pack
~0.15MPa, 121 DEG C of sterilizing 40min let cool to room temperature, after being inoculated with lucidum strain, carry out breeding in 20 DEG C of incubators, fixed
Phase observes mycelia upgrowth situation, until growing ganoderma lucidum fruitbody.
Embodiment 3
It the preparation method of cultivation of glossy ganoderma matrix, the cultivation of glossy ganoderma matrix being prepared and is carried out with this matrix in the present invention
Cultivation of glossy ganoderma, details are as follows:
One, raw material crushes
Dry walnut shell is crushed as the particle of diameter 4mm, cotton seed hulls is ground into the particle of 1.5mm, by crush maize at
Dregs of beans is ground into the particle of 1.5mm by the particle of 2mm.
Two, Hydrolysable Tannins in walnut shell is removed
Smashed walnut shell is put into fermentation vat, 8 times of walnut shell particle dry weight of water, first time soak at room temperature is added
After 2, soak is discarded；
It is added 8 times of walnut shell particle dry weight of water, after second of soak at room temperature 2h, discards soak；
Three, the condensed tannin in walnut shell is removed
5 times of walnut shell particle dry weight of water is added, adds the bean cake powder of walnut shell particle dry weight 20%, knot is sufficiently stirred
It closes, is uniformly mixed it, after room temperature 48h, pulls the walnut shell particle on upper layer out, be filtered dry moisture；
Four, culture medium is prepared
It finally establishes through screening and optimum organization using walnut shell as the matrix formulations of raw material cultivating ganoderma: walnut shell particle
40%, cotton seed hulls particle 30%, corn flour 24%, wheat bran 5%, land plaster 1%.
Five, breeding cultivation
It by the cultivation of glossy ganoderma matrix injected clear water and stirs, moisture control is in 65% pack, 0.1 after pack
~0.15MPa, 121 DEG C of sterilizing 45min let cool to room temperature, after being inoculated with lucidum strain, carry out breeding in 25 DEG C of incubators, fixed
Phase observes mycelia upgrowth situation, until growing ganoderma lucidum fruitbody.
Embodiment 4
It the preparation method of cultivation of glossy ganoderma matrix, the cultivation of glossy ganoderma matrix being prepared and is carried out with this matrix in the present invention
Cultivation of glossy ganoderma, details are as follows:
One, raw material crushes
Dry walnut shell is crushed as the particle of diameter 5mm, cotton seed hulls is ground into the particle of 2mm, by crush maize at
Dregs of beans is ground into the particle of 1.5mm by the particle of 1.5mm.
Two, Hydrolysable Tannins in walnut shell is removed
Smashed walnut shell is put into fermentation vat, 5 times of walnut shell particle dry weight of water, first time soak at room temperature is added
After 2.5h, soak is discarded；
It is added 5 times of walnut shell particle dry weight of water, after second of soak at room temperature 2.5h, discards soak；
Three, the condensed tannin in walnut shell is removed
7 times of walnut shell particle dry weight of water is added, adds the bean cake powder of walnut shell particle dry weight 10%, knot is sufficiently stirred
It closes, is uniformly mixed it, after room temperature 60h, pulls the walnut shell particle on upper layer out, be filtered dry moisture；
Four, culture medium is prepared
It finally establishes through screening and optimum organization using walnut shell as the matrix formulations of raw material cultivating ganoderma: walnut shell particle
40%, cotton seed hulls particle 30%, corn flour 24%, wheat bran 5%, land plaster 1%.
Five, breeding cultivation
It by the cultivation of glossy ganoderma matrix injected clear water and stirs, moisture control is in 60% pack, 0.1 after pack
~0.15MPa, 121 DEG C of sterilizing 40min let cool to room temperature, after being inoculated with lucidum strain, carry out breeding in 30 DEG C of incubators, fixed
Phase observes mycelia upgrowth situation, until growing ganoderma lucidum fruitbody.
Certainly, it tests according to the present invention, the cultivation of glossy ganoderma matrix specifically matches are as follows: walnut shell 40~50%, cotton seed hulls
25~30%, corn flour 20~25%, wheat bran 5~10%, land plaster 1~1.5%, are all possible within this range.
Claims (5)
1. a kind of preparation method of the cultivation of glossy ganoderma matrix based on walnut shell, which comprises the following steps:
Step 1: raw material crushes
Dry walnut shell is crushed into the particle for 3-5mm, cotton seed hulls is ground into the particle of 1-2mm, by crush maize at 1-2mm's
Dregs of beans is ground into the particle of 1-2mm by particle；
Step 2: Hydrolysable Tannins in removal walnut shell
Smashed walnut shell is put into fermentation vat, 5~10 times of walnut shell particle dry weight of water, first time soak at room temperature is added
After 2~4h, soak is discarded；
It is added 5~8 times of walnut shell particle dry weight of water, after second of 2~4h of soak at room temperature, discards soak；
Step 3: the condensed tannin in removal walnut shell
5~8 times of walnut shell particle dry weight of water is added, adds the bean cake powder of walnut shell particle dry weight 10%~20%, sufficiently
Stirring combines, and is uniformly mixed it, after 48~72h of room temperature, pulls the walnut shell particle on upper layer out, be filtered dry moisture；
Step 4: culture medium is prepared
The cultivation of glossy ganoderma matrix of preparation includes the cotton seed hulls particle prepared through step 1 and corn particle, through step 3, treated
Walnut shell particle and wheat bran, land plaster.
2. preparation method as described in claim 1, which is characterized in that in step 2, be first added 8 times of walnut shell particle dry weight
Water impregnate for the first time after, add 6 times of walnut shell particle dry weight of water second and impregnate.
3. preparation method as described in claim 1, which is characterized in that in step 3, walnut shell particle dry weight 15% is added
Bean cake powder placement processing.
4. the cultivation of glossy ganoderma matrix of preparation method preparation as described in claim 1, it is characterised in that: walnut shell particle accounting 40~
50%, cotton seed hulls particle accounting 25~30%, corn particle accounting 20~25%, wheat bran accounting 5~10%, land plaster 1~
1.5%.
5. the ganoderma lucidum cultivation method based on cultivation of glossy ganoderma matrix as claimed in claim 4, which comprises the following steps:
By the cultivation of glossy ganoderma matrix injected clear water and stirring, moisture control is packed 60~65%, 0.1 after pack~
0.15MPa, 121 DEG C of 30~45min of sterilizing let cool to room temperature, after being inoculated with lucidum strain, are educated in 20-30 DEG C of incubator
Kind, routine observation mycelia upgrowth situation, until growing ganoderma lucidum fruitbody.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100999418A (en) * | 2007-01-15 | 2007-07-18 | 西南林学院 | Method of cultivating edible fungus using walnut shell as base material |
| CN103875516A (en) * | 2014-03-07 | 2014-06-25 | 福州东星生物技术有限公司 | Method for cultivating lignicolous lucid ganoderma with walnut shells as culture medium |
| CN104446710A (en) * | 2014-12-11 | 2015-03-25 | 四川省农业科学院生物技术核技术研究所 | Lucid ganoderma cultivation medium, preparation method of lucid ganoderma cultivation medium and lucid ganoderma cultivation method |
| CN105272569A (en) * | 2015-10-15 | 2016-01-27 | 田野 | Ganoderma-lucidum culture medium and ganoderma lucidum plantation method |
| CN106242823A (en) * | 2016-08-25 | 2016-12-21 | 河北省科学院生物研究所 | A kind of planting material utilizing Pleurotus eryngii mushroom bran to produce yellow Flammulina velutiper (Fr.) Sing and preparation method thereof |
| CN108012760A (en) * | 2017-12-15 | 2018-05-11 | 河南省新乡市农业科学院 | A kind of method that edible mushroom cultivated species are made using corncob as major ingredient |
-
2018
- 2018-11-16 CN CN201811367155.3A patent/CN109220552A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100999418A (en) * | 2007-01-15 | 2007-07-18 | 西南林学院 | Method of cultivating edible fungus using walnut shell as base material |
| CN103875516A (en) * | 2014-03-07 | 2014-06-25 | 福州东星生物技术有限公司 | Method for cultivating lignicolous lucid ganoderma with walnut shells as culture medium |
| CN104446710A (en) * | 2014-12-11 | 2015-03-25 | 四川省农业科学院生物技术核技术研究所 | Lucid ganoderma cultivation medium, preparation method of lucid ganoderma cultivation medium and lucid ganoderma cultivation method |
| CN105272569A (en) * | 2015-10-15 | 2016-01-27 | 田野 | Ganoderma-lucidum culture medium and ganoderma lucidum plantation method |
| CN106242823A (en) * | 2016-08-25 | 2016-12-21 | 河北省科学院生物研究所 | A kind of planting material utilizing Pleurotus eryngii mushroom bran to produce yellow Flammulina velutiper (Fr.) Sing and preparation method thereof |
| CN108012760A (en) * | 2017-12-15 | 2018-05-11 | 河南省新乡市农业科学院 | A kind of method that edible mushroom cultivated species are made using corncob as major ingredient |
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Application publication date: 20190118 |