CN103875516B - A kind of method of cultivating red sesame using walnut shell as culture matrix - Google Patents

Abstract

Carry the method training red sesame using walnut shell as culture matrix, pulverized by walnut shell, walnut shell is placed in warm water and soaks, and discards soak after immersion, tentatively removes hydrolysable tannin; Soya bean and water are soaked jointly, at 20-28 DEG C of environment continuous dipping 10-12h, pulverizes and make soya bean slurry; Soya bean slurry and the walnut shell after water soaking are through fully stirring combination, make it mix, the airtight about 10-15 DEG C environment that is placed in places 90-98h, walnut shell after process is placed in baking oven, and dry, the walnut shell after process, cotton seed hulls, rice bran, glucose, calcium phosphate rehydration stir pack, sterilizing after pack, let cool to room temperature and inoculate red sesame bacterial classification and in 23-28 DEG C of incubator, carry out breeding, routine observation mycelial growth situation, until grow red sesame young shoot.The present invention uses semi-solid low temperature fermentation technology to destroy the bacteriostasis of tannin, and advantageously in the growth of wooden creature sesame, hydrolysable tannin is able to effective reduction.

Description

A kind of method of cultivating red sesame using walnut shell as culture matrix

[technical field]

The present invention relates to a kind of method of cultivating red sesame using walnut shell as culture matrix.

[background technology]

The glossy ganoderma artificial cultivation years development of China, have cultivating in bag also to have as culture matrix segment wood cultivated, wherein especially with segment wood cultivated wooden creature sesame closest to natural growthing condition, be considered to best in quality, in recent years due to the deflation of forest resourceies, segment wood cultivated wooden creature sesame is badly in need of alternative.

The ground such as Yunnan have the ecotopia of the raw cultivation of glossy ganoderma of wood, also abound with walnut simultaneously, walnut shell discarded after stripping walnut kernel is because forming close to timber containing a large amount of cellulose, hemicellulose, these main components of lignin, similar to the cultivation matrix-log of traditional high-quality wood creature sesame.It can be decomposed absorption by ganoderma lucidum mycelium, be applicable to the glossy ganoderma growth of the raw mushroom of wood, it is desirable cultivation matrix, but owing to containing tannin in walnut shell, tannin has stronger bacteriostasis, have certain inhibitory action to the growth of normal ganoderma lucidum mycelium, therefore walnut shell can not get effective utilization for a long time.

It take walnut shell as the method for substrate culture edible mushroom that Chinese invention patent 200710065615.2 discloses a kind of, employing be soaked in water walnut shell 24-72 hour at normal temperatures to reduce the method for tannin content merely.Method Problems existing described by this patent is: 1, because tannin is the polyhydric phenols of a class formation more complicated in plant, hydrolysable tannin and condensed tannin can be divided into by chemical constitution, they have similar biological property, all have inhibitory action to comprise inhibitory action to hypha of edible fungus bulk-growths such as glossy ganodermas to microorganism.Its hydrolysable tannin water soluble, condensed tannin is then water insoluble, therefore adopt the method to soak merely walnut shell and only can degrade, remove the partial hydrolysis tannin in tannin compound, and condensed tannin cannot be removed, condensed tannin then continues the growth suppressing the edible mushroom myceliums such as glossy ganoderma to a certain extent.2, normal temperature long-time (24-72 hour) is adopted to soak the method for walnut shell, nutriment (as polysaccharose, protein etc.) in its walnut shell is dissolved in water gradually, cause the loss of nutriment, be unfavorable for the growth of the edible mushroom myceliums such as glossy ganoderma.

In view of this, the present inventor furthers investigate for the defect of prior art, then has this case to produce.

[summary of the invention]

Technical problem to be solved by this invention is to provide a kind of method of cultivating red sesame using walnut shell as culture matrix.

The present invention is achieved in that

Cultivate a method for red sesame using walnut shell as culture matrix, comprise the steps:

Step one: raw material pre-treatment:

First walnut shell is pulverized the particle for diameter 1-5mm;

Step 2: remove hydrolysable tannin in walnut shell:

Walnut shell is placed in 35-45 DEG C of warm water and soaks 8-10h, the mass ratio of walnut shell and water is (1:3)-(1:7), discards soak, tentatively remove hydrolysable tannin after immersion;

Step 3: prepare soya bean protein liquid:

Soya bean and water are jointly soaked, the ratio of soya bean and water is (1:3)-(1:5), at 20-28 DEG C of environment continuous dipping 10-12h, pulverizes and makes soya bean slurry;

Step 4: condensed tannin in semi-solid low temperature fermentation degraded walnut shell:

Soya bean slurry through fully stirring combination, makes it mix with walnut shell after water soaking, is airtightly placed in 10-15 DEG C of environment and places 90-98h, and described soya bean slurry is (1:3)-(1:5) with the ratio of walnut shell;

Step 5: dry:

Walnut shell after process is placed in baking oven, is dried to moisture 10-13%, store;

Step 6: breeding cultivation:

Kind and the ratio of additional culture matrix is finally established through screening and optimum organization, walnut shell after process, cotton seed hulls, rice bran, glucose, calcium phosphate mass ratio are (58-61%), (21-24%), (12-15%), (1.5-2.5%), (0.8-1.2%), above-mentioned raw materials injected clear water is stirred, pack, moisture controls 60%; At 100-120 DEG C/0.1MPa sterilized under pressure 30min after pack, let cool to room temperature and inoculate red sesame bacterial classification and in 23-28 DEG C of incubator, carry out breeding, routine observation mycelial growth situation, until grow red sesame young shoot.

The invention has the advantages that: 1, utilize tannin and combined with protein to generate the compound organic matter characteristic being insoluble in water, semi-solid low temperature fermentation technology is used to destroy the bacteriostasis of tannin, soya bean also can be used as the source of nutrition of Medium for Ganoderma lucidum matter simultaneously, advantageously in the growth of red sesame.2, adopt 35-45 DEG C of emerge in worm water walnut shell 8-10h to dispel the method for hydrolysable tannin, this temperature condition is with under time conditions after measured, and walnut shell nutriment loses minimum, and hydrolysable tannin is able to effective reduction.

[embodiment]

First embodiment:

Cultivate a method for wooden creature sesame using walnut shell as culture matrix, comprise the steps:

Step one: raw material pre-treatment:

First walnut shell is pulverized the particle for diameter 2mm;

Step 2: initial hydrolysis tannin:

By walnut shell 35 DEG C of emerge in worm water 8h, the mass ratio of walnut shell and water is 1:3, removes soak after immersion;

Step 3: prepare soya bean protein liquid:

Soya bean and water are jointly soaked, the ratio of soya bean and water is 1:4, at 20 DEG C of environment continuous dipping 11h, pulverizes and makes soya bean slurry;

Step 4: semi-solid low temperature fermentation:

Soya bean slurry through fully stirring combination, makes it mix with walnut shell after water soaking, is airtightly placed in 10 DEG C of environment and places 93h, and wherein soya bean slurry is 1:4 with the mass ratio of walnut shell;

Step 5: dry:

Walnut shell after process is placed in baking oven, is dried to moisture 10%, store;

Step 6: breeding cultivation:

Kind and the ratio of additional culture matrix are: the walnut shell after process, cotton seed hulls, rice bran, glucose, calcium phosphate mass ratio are 59%, 23%, 15%, 2%, 1%, above-mentioned raw materials injected clear water is stirred, pack, moisture controls about 60%.At 100-120 DEG C/0.1MPa sterilized under pressure 30min after pack, let cool to room temperature and inoculate red sesame (No. 8, Fujian) bacterial classification in 23 DEG C of incubators, carry out breeding observation mycelial growth situation, grew red sesame young shoot to 52 days.

Second embodiment:

Cultivate a method for wooden creature sesame using walnut shell as culture matrix, comprise the steps:

Step one: raw material pre-treatment:

First walnut shell is pulverized the particle for diameter 3mm;

Step 2: initial hydrolysis tannin:

By walnut shell 40 DEG C of emerge in worm water 9h, the mass ratio of walnut shell and water is 1:5, removes soak after immersion;

Step 3: prepare soya bean protein liquid:

Soya bean and water are jointly soaked, the ratio of soya bean and water is 1:3, at 25 DEG C of environment continuous dipping 10h, pulverizes and makes soya bean slurry;

Step 4: semi-solid low temperature fermentation:

Soya bean slurry through fully stirring combination, makes it mix with walnut shell after water soaking, is airtightly placed in 15 DEG C of environment and places 96h, and wherein soya bean slurry is 1:3 with the mass ratio of walnut shell;

Step 5: dry:

Walnut shell after process is placed in baking oven, is dried to moisture 12%, store;

Step 6: breeding cultivation:

Kind and the ratio of additional culture matrix are: the walnut shell after process, cotton seed hulls, rice bran, glucose, calcium phosphate mass ratio are 60%, 23%, 14%, 2%, 1%, above-mentioned raw materials injected clear water is stirred, pack, moisture controls 60%.At 100-120 DEG C/0.1MPa sterilized under pressure 30min after pack, let cool to room temperature and inoculate red sesame (No. 8, Fujian) bacterial classification in 26 DEG C of incubators, carry out breeding observation mycelial growth situation, grew red sesame young shoot to 49 days.

3rd embodiment:

Cultivate a method for wooden creature sesame using walnut shell as culture matrix, comprise the steps:

Step one: raw material pre-treatment:

First walnut shell is pulverized the particle for diameter 5mm;

Step 2: initial hydrolysis tannin:

By walnut shell 45 DEG C of emerge in worm water 10h, the mass ratio of walnut shell and water is 1:7, removes soak after immersion;

Step 3: prepare soya bean protein liquid:

Soya bean and water are jointly soaked, the ratio of soya bean and water is 1:3, at 25 DEG C of environment continuous dipping 10h, pulverizes and makes soya bean slurry;

Step 4: semi-solid low temperature fermentation:

Soya bean slurry through fully stirring combination, makes it mix with walnut shell after water soaking, is airtightly placed in 13 DEG C of environment and places 96h, and wherein soya bean slurry is 1:3 with the mass ratio of walnut shell;

Step 5: dry:

Walnut shell after process is placed in baking oven, is dried to moisture 12%, store;

Step 6: breeding cultivation:

Kind and the ratio of additional culture matrix are: the walnut shell after process, cotton seed hulls, rice bran, glucose, calcium phosphate mass ratio are 61%, 24%, 13%, 1.5%, 0.5%, above-mentioned raw materials injected clear water is stirred, pack, moisture controls 60%.At 100-120 DEG C/0.1MPa sterilized under pressure 30min after pack, let cool to room temperature and inoculate red sesame (No. 8, Fujian) bacterial classification in 28 DEG C of incubators, carry out breeding observation mycelial growth situation, grew red sesame young shoot to 50 days.

Compare as tannin content in the culture matrix of cultivating red sesame using the walnut shell after process of the present invention as culture matrix and the undressed walnut shell of the red sesame of cultivation, consult table 1.

Tannin content compares (table 1)

Test proves: decline about 51% before comparatively processing according to the culture matrix tannin content after process of the present invention, prove the feasibility of inventing.

Culture matrix using the walnut shell after process of the present invention as the red sesame of cultivation compares as its mycelial growth situation of culture matrix of cultivating red sesame with undressed walnut shell, consults table 2.

The red sesame mycelial growth situation table of comparisons (table 2) before and after process

The above table of comparisons is visible: 1, undressed walnut shell culture matrix mycelial growth is suppressed, and cannot cultivate red sesame; Utilize the walnut shell culture matrix tannin content after process of the present invention to reduce, mycelial growth is normal.2, utilize walnut shell to cultivate the technology of red sesame as culture matrix, the method for the second embodiment, red camphorata mycelium grows time shorten, and mycelial growth is vigorous, and the pure white bacterial strain of color and luster is sturdy, grows sesame bud time shorten, therefore the most applicable red sesame cultivation.

The foregoing is only better enforcement use-case of the present invention, be not intended to limit protection scope of the present invention.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.

Claims (1)

1. cultivate a method for red sesame using walnut shell as culture matrix, it is characterized in that: comprise the steps:

Step one: raw material pre-treatment:

First walnut shell is pulverized the particle for diameter 1-5mm;

Step 2: remove hydrolysable tannin in walnut shell:

Walnut shell is placed in 35-45 DEG C of warm water and soaks 8-10h, the mass ratio of walnut shell and water is (1:3)-(1:7), discards soak, tentatively remove hydrolysable tannin after immersion;

Step 3: prepare soya bean protein liquid:

Soya bean and water are jointly soaked, the ratio of soya bean and water is (1:3)-(1:5), at 20-28 DEG C of environment continuous dipping 10-12h, pulverizes and makes soya bean slurry;

Step 4: condensed tannin in semi-solid low temperature fermentation degraded walnut shell:

Soya bean slurry through fully stirring combination, makes it mix with walnut shell after water soaking, is airtightly placed in 10-15 DEG C of environment and places 90-98h, and described soya bean slurry is (1:3)-(1:5) with the ratio of walnut shell;

Step 5: dry:

Walnut shell after process is placed in baking oven, is dried to moisture 10-13%, store;

Step 6: breeding cultivation:

Kind and the ratio of additional culture matrix is finally established through screening and optimum organization, walnut shell after process, cotton seed hulls, rice bran, glucose, calcium phosphate mass ratio are (58-61%), (21-24%), (12-15%), (1.5-2.5%), (0.8-1.2%), above-mentioned raw materials injected clear water is stirred, pack, moisture controls 60%; At 100-120 DEG C/0.1MPa sterilized under pressure 30min after pack, let cool to room temperature and inoculate red sesame bacterial classification and in 23-28 DEG C of incubator, carry out breeding, routine observation mycelial growth situation, until grow red sesame young shoot.