Disclosure of Invention
An object of the present invention is to solve the above-mentioned problems and to provide at least the advantages which will be described later.
The invention also aims to provide a standardized earthworm breeding method, which can promote the rapid growth of earthworms and can obtain high-quality earthworms.
To achieve these objects and other advantages in accordance with the purpose of the invention, there is provided a method for standardized breeding of earthworms, comprising the steps of:
s1, building an earthworm bed with the length of 3m, the width of 2m and the height of 0.5m in the breeding room, and paving breeding soil with the height of 10cm on the earthworm bed;
s2, paving a breeding base material on the breeding soil, and then putting earthworm seedlings on the earthworm bed;
the preparation method of the culture soil comprises the following steps:
a1, mixing and crushing 5-10 parts by weight of macadamia shell, 5-8 parts by weight of coconut shell, 3-6 parts by weight of almond shell and 1-5 parts by weight of walnut shell, soaking in 25 parts by weight of 10% acetic acid solution for 5min, filtering and drying to obtain a first mixed material; then heating the first mixed material from room temperature to 200 ℃ at a speed of 4 ℃/min, and keeping the temperature at 200 ℃ for 3-5 h; then raising the temperature from 200 ℃ to 500 ℃ at the speed of 6 ℃/min, then lowering the temperature from 500 ℃ to 200 ℃ at the speed of 8 ℃/min, and repeating the cycle for 5 times; finally, cooling the mixture from 200 ℃ to room temperature at the speed of 6 ℃/min to obtain a second material;
a2, uniformly mixing the soil, the second material, the biochar and the bamboo fiber in a mass ratio of 10:1:0.5:0.8, and then spraying a sterilizing solution to obtain the culture soil; the mass of the sterilizing liquid is 0.1% of that of soil;
the preparation method of the sterilizing liquid comprises the following steps:
b1, crushing 5-10 parts by weight of dandelion, 3-5 parts by weight of sculellaria barbata, 4-8 parts by weight of subprostrate sophora, 3-6 parts by weight of Chinese sage herb, 3-6 parts by weight of caulis spatholobi, 1-5 parts by weight of common camptotheca root and 3-6 parts by weight of seaweed, dissolving in 100 parts by weight of water, adding 0.2 part by weight of cellulase and 0.1 part by weight of pectinase, soaking for 1-2 min, and inactivating enzymes to obtain a first mixed material;
b2, placing the first mixed material in a closed container, keeping the temperature in the container at 90-95 ℃, introducing saturated water vapor into the container to enable the pressure in the container to be 1.5Mpa, keeping the pressure for 10-15 hours, reducing the pressure in the container to be 0.5Kpa, keeping the pressure for 1-2 min, taking out the first mixed material, and filtering to obtain sterilizing liquid and filter residues;
the preparation method of the culture base material comprises the following steps:
c1, mixing and crushing 5 parts of shrimp shell, 3 parts of crab shell, 5 parts of pig bone and 3 parts of chicken bone, adding 0.5 part of pepsin and 2 parts of trypsin for enzymolysis for 6 hours, and inactivating enzyme to obtain an zymolyte;
c2, uniformly mixing 35 parts by weight of pig manure, 10 parts by weight of plant straws, 5 parts by weight of wheat bran, 3 parts by weight of leavening agent, 40 parts by weight of water, filter residue in B2 and zymolyte in C1, and then carrying out aerobic fermentation for 30-40 days to obtain the culture base material.
Preferably, in the standardized earthworm breeding method, the plant straw is one of corn straw, wheat straw and rice straw, and before fermentation, the plant straw is crushed and then is soaked in a 10% sodium hydroxide solution for 5-10 min.
Preferably, in the standardized earthworm breeding method, the leavening agent comprises a mixture of lactic acid bacteria, bacillus subtilis, yeast, aspergillus niger and trichoderma viride, and the mass ratio of the lactic acid bacteria, the bacillus subtilis, the yeast, the aspergillus niger and the trichoderma viride in the leavening agent is 1:0.5:2:0.2: 0.5.
Preferably, in the standardized earthworm breeding method, the enzymolysis temperature in C1 is 35-37 ℃.
Preferably, in the standardized earthworm breeding method, the aerobic fermentation temperature is 28-30 ℃, and 10% oxygen by volume fraction is introduced into the fermentation product every 2 days.
Preferably, in the standardized earthworm breeding method, the breeding base material is laid in S2, and the phagostimulant is further sprayed, and the preparation method of the phagostimulant comprises the following steps: uniformly mixing the crushed lemon, the crushed apple and water according to the mass ratio of 1:1:8, heating at 50-60 ℃ for 5-10 min, and filtering to obtain the phagostimulant.
The invention at least comprises the following beneficial effects:
1. according to the standardized earthworm breeding method, breeding soil is laid on an earthworm bed, a porous second material is obtained by calcining macadamia shell, coconut shell and the like in a segmented and multi-step manner, then the second material, biochar and bamboo fiber are uniformly mixed, and then sterilization liquid is sprayed to obtain the breeding soil; the biochar and the bamboo fiber have porosity and a sterilizing effect, and the second material, the biochar and the bamboo fiber can loosen and make the culture soil porous, so that the growth of earthworms is facilitated; the breeding soil is also sprayed with a sterilizing liquid, so that fungi in the environment can be killed, the morbidity of earthworms is reduced, the mortality is reduced, and the yield and the quality of the earthworms are improved.
2. The invention relates to a method for standardized breeding of earthworms, which comprises the steps of carrying out enzymolysis on Chinese herbal medicines such as dandelion, sculellaria barbata and the like by using cellulase and pectinase when preparing a sterilizing solution, so that cell walls of the Chinese herbal medicines are damaged, then placing the Chinese herbal medicines subjected to enzymolysis in a closed container, firstly introducing high-pressure steam, then instantly relieving pressure, penetrating the high-pressure steam into the Chinese herbal medicines after the damaged cell walls in the process of introducing the high-pressure steam, and then instantly relieving pressure, wherein in the process, the high-pressure steam penetrating into the Chinese herbal medicines can be sprayed out from the interior of the Chinese herbal medicines, so that the blasting effect is achieved, medicinal components in the Chinese herbal medicines can be released as far as possible by the method, and the utilization rate of the Chinese herbal.
3. According to the standardized earthworm breeding method, breeding base materials are further applied, animal feeds such as shrimp shells, crab shells, pig bones and the like are subjected to enzymolysis through pepsin and trypsin to obtain zymolyte, then pig manure, plant straws, wheat bran, the zymolyte and filter residues are fermented to obtain the breeding base materials, the obtained breeding base materials are balanced in nutrition and can promote the growth of earthworms, the breeding base materials also take the filter residues as raw materials, the breeding base materials have a certain health-care effect, and the death rate of the earthworms is reduced.
4. According to the standardized earthworm breeding method, the food calling liquid is further added, the lemon and the apple are boiled in water to obtain the food calling liquid, and the food calling liquid has sour and sweet taste and can increase the appetite of the earthworms.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
Example 1
A standardized earthworm breeding method comprises the following steps:
s1, building an earthworm bed with the length of 3m, the width of 2m and the height of 0.5m in the breeding room, and paving breeding soil with the height of 10cm on the earthworm bed;
s2, paving a breeding base material on the breeding soil, and then putting earthworm seedlings on the earthworm bed;
the preparation method of the culture soil comprises the following steps:
a1, mixing and crushing 5 parts by weight of macadamia shell, 5 parts by weight of coconut shell, 3 parts by weight of almond shell and 1 part by weight of walnut shell, soaking in 25 parts by weight of 10% acetic acid solution for 5min, filtering and drying to obtain a first mixed material; then heating the first mixed material from room temperature to 200 ℃ at a speed of 4 ℃/min, and keeping the temperature at 200 ℃ for 3-5 h; then raising the temperature from 200 ℃ to 500 ℃ at the speed of 6 ℃/min, then lowering the temperature from 500 ℃ to 200 ℃ at the speed of 8 ℃/min, and repeating the cycle for 5 times; finally, cooling the mixture from 200 ℃ to room temperature at the speed of 6 ℃/min to obtain a second material;
a2, uniformly mixing the soil, the second material, the biochar and the bamboo fiber in a mass ratio of 10:1:0.5:0.8, and then spraying a sterilizing solution to obtain the culture soil; the mass of the sterilizing liquid is 0.1% of that of soil;
the preparation method of the sterilizing liquid comprises the following steps:
b1, crushing 5 parts by weight of dandelion, 3 parts by weight of sculellaria barbata, 4 parts by weight of subprostrate sophora, 3 parts by weight of Chinese sage, 3 parts by weight of caulis spatholobi, 1 part by weight of common camptotheca root and 3 parts by weight of seaweed, dissolving in 100 parts by weight of water, adding 0.2 part by weight of cellulase and 0.1 part by weight of pectinase, soaking for 1min, and inactivating enzymes to obtain a first mixed material;
b2, placing the first mixed material in a closed container, maintaining the temperature in the container at 90 ℃, introducing saturated water vapor into the container to enable the pressure in the container to be 1.5Mpa, keeping the pressure for 10 hours, reducing the pressure in the container to be 0.5Kpa, keeping the pressure for 1min, taking out the first mixed material, and filtering to obtain a sterilizing solution and filter residues;
the preparation method of the culture base material comprises the following steps:
c1, mixing and crushing 5 parts of shrimp shell, 3 parts of crab shell, 5 parts of pig bone and 3 parts of chicken bone, adding 0.5 part of pepsin and 2 parts of trypsin for enzymolysis for 6 hours, and inactivating enzyme to obtain an zymolyte;
c2, uniformly mixing 35 parts of pig manure, 10 parts of plant straws, 5 parts of wheat bran, 3 parts of leavening agent, 40 parts of water, filter residue in B2 and zymolyte in C1, and then performing aerobic fermentation for 30 days to obtain the culture base material.
According to the standardized earthworm breeding method, the plant straw is one of corn straw, wheat straw and rice straw, and before fermentation, the plant straw is crushed and then is soaked in a 10% sodium hydroxide solution for 5 min.
According to the standardized earthworm breeding method, the leavening agent comprises a mixture of lactic acid bacteria, bacillus subtilis, saccharomycetes, aspergillus niger and trichoderma viride, and the mass ratio of the lactic acid bacteria to the bacillus subtilis to the saccharomycetes to the aspergillus niger to the trichoderma viride is 1:0.5:2:0.2: 0.5.
In the standardized earthworm breeding method, the enzymolysis temperature in C1 is 35 ℃.
According to the standardized earthworm breeding method, the aerobic fermentation temperature is 28 ℃, and oxygen with the volume fraction of 10% is introduced into the fermentation product every 2 days.
In the standardized earthworm breeding method, the breeding base material is laid in S2, and the attractant is also sprayed, and the preparation method of the attractant comprises the following steps: uniformly mixing the crushed lemon, the crushed apple and water according to the mass ratio of 1:1:8, heating at 50 ℃ for 5min, and filtering to obtain the phagostimulant.
Example 2
A standardized earthworm breeding method comprises the following steps:
s1, building an earthworm bed with the length of 3m, the width of 2m and the height of 0.5m in the breeding room, and paving breeding soil with the height of 10cm on the earthworm bed;
s2, paving a breeding base material on the breeding soil, and then putting earthworm seedlings on the earthworm bed;
the preparation method of the culture soil comprises the following steps:
a1, mixing and crushing 10 parts by weight of macadamia shell, 8 parts by weight of coconut shell, 6 parts by weight of almond shell and 5 parts by weight of walnut shell, soaking the mixture in 25 parts by weight of 10% acetic acid solution for 5min, and filtering and drying to obtain a first mixed material; then heating the first mixed material from room temperature to 200 ℃ at a speed of 4 ℃/min, and keeping the temperature at 200 ℃ for 3-5 h; then raising the temperature from 200 ℃ to 500 ℃ at the speed of 6 ℃/min, then lowering the temperature from 500 ℃ to 200 ℃ at the speed of 8 ℃/min, and repeating the cycle for 5 times; finally, cooling the mixture from 200 ℃ to room temperature at the speed of 6 ℃/min to obtain a second material;
a2, uniformly mixing the soil, the second material, the biochar and the bamboo fiber in a mass ratio of 10:1:0.5:0.8, and then spraying a sterilizing solution to obtain the culture soil; the mass of the sterilizing liquid is 0.1% of that of soil;
the preparation method of the sterilizing liquid comprises the following steps:
b1, crushing 10 parts by weight of dandelion, 5 parts by weight of sculellaria barbata, 8 parts by weight of subprostrate sophora, 6 parts by weight of Chinese sage herb, 6 parts by weight of suberect spatholobus stem, 5 parts by weight of common camptotheca root and 6 parts by weight of seaweed, dissolving in 100 parts by weight of water, adding 0.2 part by weight of cellulase and 0.1 part by weight of pectinase, soaking for 2min, and inactivating enzymes to obtain a first mixed material;
b2, placing the first mixed material in a closed container, keeping the temperature in the container at 95 ℃, introducing saturated water vapor into the container to enable the pressure in the container to be 1.5Mpa, keeping the pressure for 15 hours, reducing the pressure in the container to be 0.5Kpa, keeping the pressure for 2min, taking out the first mixed material, and filtering to obtain a sterilizing liquid and filter residues;
the preparation method of the culture base material comprises the following steps:
c1, mixing and crushing 5 parts of shrimp shell, 3 parts of crab shell, 5 parts of pig bone and 3 parts of chicken bone, adding 0.5 part of pepsin and 2 parts of trypsin for enzymolysis for 6 hours, and inactivating enzyme to obtain an zymolyte;
c2, uniformly mixing 35 parts of pig manure, 10 parts of plant straws, 5 parts of wheat bran, 3 parts of leavening agent, 40 parts of water, filter residue in B2 and zymolyte in C1, and then performing aerobic fermentation for 40 days to obtain the culture base material.
According to the standardized earthworm breeding method, the plant straw is one of corn straw, wheat straw and rice straw, and before fermentation, the plant straw is crushed and then is soaked in a 10% sodium hydroxide solution for 10 min.
According to the standardized earthworm breeding method, the leavening agent comprises a mixture of lactic acid bacteria, bacillus subtilis, saccharomycetes, aspergillus niger and trichoderma viride, and the mass ratio of the lactic acid bacteria to the bacillus subtilis to the saccharomycetes to the aspergillus niger to the trichoderma viride is 1:0.5:2:0.2: 0.5.
According to the standardized earthworm breeding method, the enzymolysis temperature in C1 is 37 ℃.
According to the standardized earthworm breeding method, the aerobic fermentation temperature is 30 ℃, and oxygen with the volume fraction of 10% is introduced into the fermentation product every 2 days.
In the standardized earthworm breeding method, the breeding base material is laid in S2, and the attractant is also sprayed, and the preparation method of the attractant comprises the following steps: uniformly mixing the crushed lemon, the crushed apple and water according to the mass ratio of 1:1:8, heating at 60 ℃ for 10min, and filtering to obtain the phagostimulant.
Comparative example 1
The difference from example 1 is that the culture soil contained only soil.
Comparative example 2
The difference from the example 1 is that the culture base material is conventional animal feed and plant feed which are mixed according to the ratio of 1:2, wherein the animal feed is pig manure, and the plant feed is corn stalks.
The results of experiments on the average length, weight and protein content of 10 earthworms randomly tested while the reproduction times and mortality of the earthworms were respectively tested by breeding the earthworms according to the methods of example 1 and comparative examples 1-2 are shown in table 1.
TABLE 1 Effect of different examples on earthworm growth
|
Multiple of reproduction
|
Mortality (%)
|
Length (cm)
|
Average body weight (g)
|
Protein content (%)
|
Example 1
|
42
|
0.86
|
13.5
|
47.6
|
53.2
|
Comparative example 1
|
32
|
1.23
|
13.2
|
45.3
|
48.3
|
Comparative example 2
|
34
|
1.05
|
12.9
|
40.2
|
45.4 |
As can be seen from Table 1, the breeding soil and the breeding base material of the invention can promote the rapid breeding of the earthworms and promote the rapid growth of the earthworms, and the earthworms bred by the method have higher average weight and higher protein content, thereby improving the quality of the earthworms.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.