CN105016981A - Extraction method for resveratrol in peanut sprouts - Google Patents
Extraction method for resveratrol in peanut sprouts Download PDFInfo
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- CN105016981A CN105016981A CN201510393064.7A CN201510393064A CN105016981A CN 105016981 A CN105016981 A CN 105016981A CN 201510393064 A CN201510393064 A CN 201510393064A CN 105016981 A CN105016981 A CN 105016981A
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- resveratrol
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
- C07C37/004—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring by obtaining phenols from plant material or from animal material
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
Abstract
The invention relates to the field of plant component extraction, particularly an extraction method for resveratrol in peanut sprouts. Aiming at the existing resveratrol extraction situation, the invention aims to provide a resveratrol extraction method which is simple to extract, convenient and feasible and convenient for large-scaled production, wherein the raw materials are abundant in soure and the resveratrol content is high. According to the technical scheme provided by the invention, the extraction method for resveratrol in peanut sprouts comprises the following steps: S1, peanut seed treatment: S2, pre-treatment of resveratrol extraction from the peanut sprouts; S3, squeezing of quick-frozen weak peanut sprouts; S4, water extraction of peanut sprout fiber; S5, acid-catalyzed hydrolysi; S6, purification; and S7, crystallization. According to the extraction method for resveratrol provided by the invention, the raw materials are abundant in source, the extraction method for resveratrol is low in production cost, strong in operability and suitable for large-scaled production, and moreover, the purity of the obtained resveratrol is high.
Description
Technical field
The present invention relates to plant component extraction field, particularly the extracting method of trans-resveratrol in a kind of peanut sprout.
Background technology
Trans-resveratrol is polyphenolic compound, trans-resveratrol (Res) chemical name 3,4,5-trihydroxyies-toluylene, molecular formula C14H12O3, fusing point 256 ~ 257 DEG C.Mainly exist with polydatin (PDT) form in plant materials, be mainly derived from the plants such as grape (red wine), giant knotweed, peanut, mulberry fruit.
Trans-resveratrol is a kind of biological very strong natural polyphenol class material, and being also called resvertrol, is the chemopreventive agent of tumour, is also to reduction platelet aggregation, the chemopreventive agent of prevention and therapy atherosclerosis, cardiovascular and cerebrovascular diseases.The verified beneficial effect had cardiovascular disorder and cancer of the experimental study of trans-resveratrol.The sickness rate of blood fat and coronary heart disease can be reduced simultaneously, special protection function is risen to cardiovascular systems; Regulate the ratio of low-density lipoprotein (LDL); Reduce the effects such as platelet aggregation; Delay senility;
Because trans-resveratrol has multiple biology and pharmacologically active, it is made to be widely used in the fields such as food, medicine, healthcare products, makeup.It is very large and increases severely with day that trans-resveratrol has excellent pharmacologically active and its market requirement of nourishing function, has most countries and area all develops trans-resveratrol and goods thereof.The U.S. using trans-resveratrol as dietary supplements, Japan using the trans-resveratrol from plant extract as foodstuff additive, the natural health care of lipopenicillinase beauty treatment will have been made by China containing the plant milk extract of trans-resveratrol.
How a large amount of extractions and large-scale production trans-resveratrol are the targets that researcher is constantly pursued always; mostly traditional method is to extract trans-resveratrol from giant knotweed; but giant knotweed growth cycle is long; the content that generally will grow 3 years trans-resveratrols just can reach the amount of gathering; and the high giant knotweed of content mostly is wild giant knotweed; along with excavating in a large number of wild giant knotweed, giant knotweed resource reduces in a large number, and a large amount of extractions becoming giant knotweed trans-resveratrol propose a difficult problem.
Everybody has generally acknowledged that the compatriots making traditional habit drink white wine are in the acceptance to health theory, also start to drink grape wine containing trans-resveratrol in grape, Semen Vitis viniferae, grape wine (red grape); Research proves: the grape wine drinking HUMAN HEALTH receiving amount does not have effect; Extract with Pericarpium Vitis viniferae, Semen Vitis viniferae that trans-resveratrol is good does not produce on a large scale.
Research shows Testa arachidis hypogaeae also containing a large amount of trans-resveratrols, but Testa arachidis hypogaeae collects difficulty, but studying the feasible scarcity often because of raw material in actual scale operation in theory causes actual production to implement.
The research in Pennsylvania State Univ-Univ Park USA professor Pei Ni and college professor Qiu Yi source, Taiwan Chiayi etc., containing trans-resveratrol in peanut, its content is the thirtyfold of grape.Resveratrol content in grape only has 1,000,000 usually/, and the Resveratrol content in peanut is up to 30/1000000ths.Qiu Yiyuan points out, general red wine is about containing the trans-resveratrol of one to two ppm, but peanut sprout is containing 30 ppm, after particular form is cultivated, more can rise to 3 hundred to five hundred ppm, after peanut germinates, its Resveratrol content increases sharply, assay shows, the Resveratrol content in peanut sprout is 100 times of Semen arachidis hypogaeae, is also 100 times that exceed Resveratrol content in grape; Trans-resveratrol is extracted in peanut vernalization, really can realize the large-scale production of trans-resveratrol.
Summary of the invention
Based on above reason, the object of this invention is to provide a kind of abundant raw material source, and Resveratrol content is high, extracts easy, facilitate feasible, be convenient to the extracting method of the trans-resveratrol of scale operation: the technical scheme realizing above-mentioned purpose is:
An extracting method for trans-resveratrol in peanut sprout, is made up of following steps:
Step one, peanut seed process:
Selection grain is large, full seed, color and luster is fresh, epidermis is smooth, the peanut seed of the consistent high-quality of shape, the fresh peanut chosen is put into container add water, the water yield is peanut weight 40% ~ 50%, in the warm water of 20 ~ 25 DEG C, soak peanut kind 12 hours, 25 DEG C of constant temperature germinating boxs are loaded after seed soaking, within 4 hours, drench the warm water of 25 DEG C, sprout after first time bubble bud 3d, once select having urged the seed of bud, remove the seed do not germinateed, the seed germinateed is carried out secondary vernalization, in 25 DEG C of constant temperature germinating boxs, 8d peanut sprout grows to 7 centimetres, scarlet pushes away by peanut sprout, when leaf bud exposes bud lobe, vernalization completes, now in peanut sprout, Resveratrol content reaches maximum,
Step 2, peanut sprout trans-resveratrol extract pre-treatment:
Drained by the peanut sprout of step one gained, suddenly frozen fast by peanut sprout after draining, freezing temperature is frozen 4 hours for-30 DEG C, takes out to be put in the warm water of 20 ~ 25 DEG C and thaws, and has thawed to dry water with whizzer; Repetitive operation quick-frozen once again; Peanut sprout after pre-freeze is put into vacuum vessel, by by vacuum system, window internal pressure is dropped to below triple point, by heating system heat supply to material, material moisture is evaporated to gradually half-dried in weak shape; Weak peanut sprout extruding after step 3, quick-frozen:
Will in weak shape peanut sprout by hand or mechanical means extruding in threadiness for subsequent use;
The water extraction of step 4, peanut sprout fiber:
Peanut sprout fiber is put into 100 DEG C of hot water, decoct 35 minutes, filter to obtain the aqueous solution, repeat aforesaid operations 3 times; The filtrate of gained is merged vacuum concentration to 30% for subsequent use;
Step 5: acid-catalyzed hydrolysis,
By 30% concentrated solution of step 4 gained by volume proportioning be 8% dilute hydrochloric acid at 60 DEG C, be hydrolyzed 6 hours with the amount of 8 times, reaction after cooling, for subsequent use
Step 6: purifying:
The cooling fluid macroporous adsorptive resins of anticipating after reaction is carried out chromatographic separation: macroporous adsorption resin chromatography process comprises: 1, macroporous adsorbent resin pre-treatment: the process undertaken by product operation instruction method; 2, raise up absorption: after the cooling fluid suction filtration of step 5 gained, on the macroporous adsorptive resins of anticipating carry out loading absorption 3, chromatographic elution: after extracting solution loading, the ethanol elution of alcohol 95, follows the tracks of with ripple layer chromatography and detects, and collects trans-resveratrol section elutriant
Step 7: by the trans-resveratrol elutriant collected, adopts charcoal absorption decolouring, then obtains resveratrol recrystallized by spray-drying process.
In step one, peanut seed treatment temp is too high, though growth is fast, sprout is thin and delicate, easily aging; Temperature is too low, then growth is slow, time long easy rotten bud or cotyledon the first transaction of a day's business polypetalous, poor quality, through comparative experimental research, the peanut sprout obtained by method of the present invention now in peanut sprout Resveratrol content the highest, subsequent operations dialogue Li Lu ethanol-extracted impact is little.
In step 2, adopt cold method that the moisture in peanut sprout is removed substantially, and do not affect the compositions such as the trans-resveratrol in peanut sprout; Can not impact follow-up operation.
Be hydrolyzed with sour water again after the present invention's water extraction, again further across using alcohol wash-out, trans-resveratrol in raw material not only can be made to be extracted fully, the trans-resveratrol glycoside in peanut sprout can also be made to be hydrolyzed to the aglycon of free state simultaneously, the content of further increase trans-resveratrol, can also be removed the impurity in extract by the present invention, be that trans-resveratrol purity is higher, quality is more stable simultaneously.
Extract trans-resveratrol abundant raw material source by present method, be produced into this locality, workable, be applicable to scale operation, extract gained trans-resveratrol purity high simultaneously,
specific implementation method
Once the content of invention is described in detail in conjunction with the embodiments
An extracting method for trans-resveratrol in peanut sprout, is made up of following steps:
Step one, peanut seed process:
Selection grain is large, full seed, color and luster is fresh, epidermis is smooth, the peanut seed of the consistent high-quality of shape, the fresh peanut chosen is put into container add water, the water yield is peanut weight 40% ~ 50%, in the warm water of 20 ~ 25 DEG C, soak peanut kind 12 hours, 25 DEG C of constant temperature germinating boxs are loaded after seed soaking, within 4 hours, drench the warm water of 25 DEG C, sprout after first time bubble bud 3d, once select having urged the seed of bud, remove the seed do not germinateed, the seed germinateed is carried out secondary vernalization, in 25 DEG C of constant temperature germinating boxs, 8d peanut sprout grows to 7 centimetres, scarlet pushes away by peanut sprout, when leaf bud exposes bud lobe, vernalization completes, now in peanut sprout, Resveratrol content reaches maximum,
Step 2, peanut sprout trans-resveratrol extract pre-treatment:
Drained by the peanut sprout of step one gained, suddenly frozen fast by peanut sprout after draining, freezing temperature is frozen 4 hours for-30 DEG C, takes out to be put in the warm water of 20 ~ 25 DEG C and thaws, and has thawed to dry water with whizzer; Repetitive operation quick-frozen once again; Peanut sprout after pre-freeze is put into vacuum vessel, by by vacuum system, window internal pressure is dropped to below triple point, by heating system heat supply to material, material moisture is evaporated to gradually half-dried in weak shape, moisture in peanut sprout is removed substantially, and does not affect the compositions such as the trans-resveratrol in peanut sprout;
Weak peanut sprout extruding after step 3, quick-frozen:
Will in weak shape peanut sprout by hand or mechanical means extruding in threadiness for subsequent use;
The water extraction of step 4, peanut sprout fiber:
Peanut sprout fiber is put into 100 DEG C of hot water, decoct 35 minutes, filter to obtain the aqueous solution, repeat aforesaid operations 3 times; The filtrate of gained is merged vacuum concentration to 30% for subsequent use;
Step 5: acid-catalyzed hydrolysis,
By 30% concentrated solution of step 4 gained by volume proportioning be 8% dilute hydrochloric acid at 60 DEG C, be hydrolyzed 6 hours with the amount of 8 times, reaction after cooling, for subsequent use
Step 6: purifying:
The cooling fluid macroporous adsorptive resins of anticipating after reaction is carried out chromatographic separation: macroporous adsorption resin chromatography process comprises: 1, macroporous adsorbent resin pre-treatment: the process undertaken by product operation instruction method; 2, raise up absorption: after the cooling fluid suction filtration of step 5 gained, on the macroporous adsorptive resins of anticipating carry out loading absorption 3, chromatographic elution: after extracting solution loading, the ethanol elution of alcohol 95, follows the tracks of with ripple layer chromatography and detects, and collects trans-resveratrol section elutriant
Step 7: by the trans-resveratrol elutriant collected, adopts charcoal absorption decolouring, then obtains resveratrol recrystallized by spray-drying process.
Claims (1)
1. the extracting method of trans-resveratrol in peanut sprout, is characterized in that being made up of following steps:
Step one, peanut seed process:
Selection grain is large, full seed, color and luster is fresh, epidermis is smooth, the peanut seed of the consistent high-quality of shape, the fresh peanut chosen is put into container add water, the water yield is peanut weight 40% ~ 50%, in the warm water of 20 ~ 25 DEG C, soak peanut kind 12 hours, 25 DEG C of constant temperature germinating boxs are loaded after seed soaking, within 4 hours, drench the warm water of 25 DEG C, sprout after first time bubble bud 3d, once select having urged the seed of bud, remove the seed do not germinateed, the seed germinateed is carried out secondary vernalization, in 25 DEG C of constant temperature germinating boxs, 8d peanut sprout grows to 7 centimetres, scarlet pushes away by peanut sprout, when leaf bud exposes bud lobe, vernalization completes, now in peanut sprout, Resveratrol content reaches maximum,
Step 2, peanut sprout trans-resveratrol extract pre-treatment:
Drained by the peanut sprout of step one gained, suddenly frozen fast by peanut sprout after draining, freezing temperature is frozen 4 hours for-30 DEG C, takes out to be put in the warm water of 20 ~ 25 DEG C and thaws, and has thawed to dry water with whizzer; Repetitive operation quick-frozen once again; Peanut sprout after pre-freeze is put into vacuum vessel, by by vacuum system, window internal pressure is dropped to below triple point, by heating system heat supply to material, material moisture is evaporated to gradually half-dried in weak shape, moisture in peanut sprout is removed substantially, and does not affect the compositions such as the trans-resveratrol in peanut sprout;
Weak peanut sprout extruding after step 3, quick-frozen:
Will in weak shape peanut sprout by hand or mechanical means extruding in threadiness for subsequent use;
The water extraction of step 4, peanut sprout fiber:
Peanut sprout fiber is put into 100 DEG C of hot water, decoct 35 minutes, filter to obtain the aqueous solution, repeat aforesaid operations 3 times; The filtrate of gained is merged vacuum concentration to 30% for subsequent use;
Step 5, acid-catalyzed hydrolysis:
By 30% concentrated solution of step 4 gained by volume proportioning be 8% dilute hydrochloric acid at 60 DEG C, be hydrolyzed 6 hours with the amount of 8 times, reaction after cooling, for subsequent use;
Step 6, purifying:
The cooling fluid macroporous adsorptive resins of anticipating after reaction is carried out chromatographic separation: macroporous adsorption resin chromatography process comprises: 1, macroporous adsorbent resin pre-treatment: the process undertaken by product operation instruction method; 2, raise up absorption: after the cooling fluid suction filtration of step 5 gained, on the macroporous adsorptive resins of anticipating carry out loading absorption 3, chromatographic elution: after extracting solution loading, the ethanol elution of alcohol 95, follows the tracks of with ripple layer chromatography and detects, and collects trans-resveratrol section elutriant;
Step 7, the trans-resveratrol elutriant that will collect, adopt charcoal absorption decolouring, then obtain resveratrol recrystallized by spray-drying process.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106233873A (en) * | 2016-08-04 | 2016-12-21 | 巢湖市粮食协会 | A kind of method improving peanut seed germination percentage |
| WO2017137957A1 (en) * | 2016-02-11 | 2017-08-17 | Tripathi Vinaykumar | Colloidally stable resveratrol nanoparticles with improved bioavailability and half-life and synthesis thereof |
| WO2018042324A1 (en) * | 2016-08-29 | 2018-03-08 | Tripathi Vinaykumar | Modified resveratrol composition and use thereof |
| CN111296289A (en) * | 2020-03-12 | 2020-06-19 | 蚌埠学院 | Method for improving resveratrol content in peanut sprouts by cell suspension culture technology |
| US11033514B2 (en) | 2016-08-29 | 2021-06-15 | Vinaykumar TRIPATHI | Modified resveratrol composition and use thereof |
Citations (3)
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| CN104003849A (en) * | 2013-09-17 | 2014-08-27 | 黄桂淑 | Extraction method of resveratrol from peanut buds |
| CN103999748A (en) * | 2013-09-17 | 2014-08-27 | 黄桂淑 | Method for obtaining resveratrol-enriched peanut seedlings |
| CN104023565A (en) * | 2012-08-29 | 2014-09-03 | 农业公司法人株式会社长寿菜 | Method for extracting peanut sprouts having increased resveratrol content |
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- 2015-07-07 CN CN201510393064.7A patent/CN105016981B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104023565A (en) * | 2012-08-29 | 2014-09-03 | 农业公司法人株式会社长寿菜 | Method for extracting peanut sprouts having increased resveratrol content |
| CN104003849A (en) * | 2013-09-17 | 2014-08-27 | 黄桂淑 | Extraction method of resveratrol from peanut buds |
| CN103999748A (en) * | 2013-09-17 | 2014-08-27 | 黄桂淑 | Method for obtaining resveratrol-enriched peanut seedlings |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017137957A1 (en) * | 2016-02-11 | 2017-08-17 | Tripathi Vinaykumar | Colloidally stable resveratrol nanoparticles with improved bioavailability and half-life and synthesis thereof |
| CN106233873A (en) * | 2016-08-04 | 2016-12-21 | 巢湖市粮食协会 | A kind of method improving peanut seed germination percentage |
| WO2018042324A1 (en) * | 2016-08-29 | 2018-03-08 | Tripathi Vinaykumar | Modified resveratrol composition and use thereof |
| US11033514B2 (en) | 2016-08-29 | 2021-06-15 | Vinaykumar TRIPATHI | Modified resveratrol composition and use thereof |
| CN111296289A (en) * | 2020-03-12 | 2020-06-19 | 蚌埠学院 | Method for improving resveratrol content in peanut sprouts by cell suspension culture technology |
| CN111296289B (en) * | 2020-03-12 | 2021-08-31 | 蚌埠学院 | Method for improving resveratrol content in peanut sprouts by cell suspension culture technology |
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