CN105016981B - The extracting method of resveratrol in a kind of peanut sprout - Google Patents
The extracting method of resveratrol in a kind of peanut sprout Download PDFInfo
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- CN105016981B CN105016981B CN201510393064.7A CN201510393064A CN105016981B CN 105016981 B CN105016981 B CN 105016981B CN 201510393064 A CN201510393064 A CN 201510393064A CN 105016981 B CN105016981 B CN 105016981B
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- resveratrol
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- peanut sprout
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
- C07C37/004—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring by obtaining phenols from plant material or from animal material
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
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- Life Sciences & Earth Sciences (AREA)
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- Coloring Foods And Improving Nutritive Qualities (AREA)
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Abstract
The present invention relates to plant component extraction field, the extracting method of resveratrol in particularly a kind of peanut sprout, present situation is extracted for existing resveratrol, it is abundant it is an object of the invention to provide a kind of raw material sources, and Resveratrol content is high, extract easy, facilitate feasible, be easy to the extracting method of the resveratrol of large-scale production：Realizing the technical scheme of above-mentioned purpose is：The extracting method of resveratrol, is comprised the steps of in a kind of peanut sprout：Step 1: peanut seed is handled：Step 2: peanut sprout resveratrol extracts pre-treatment：Step 3: the weak peanut sprout extruding after quick-frozen：Step 4: the water extraction of peanut sprout fiber：Step 5：Acid-catalyzed hydrolysis；Step 6：Purifying：Step 7：Crystallization：Resveratrol raw material sources are extracted by this method to enrich, and local, workable, suitable large-scale production are produced into, while it is high to extract gained resveratrol purity.
Description
Technical field
The present invention relates to plant component extraction field, the extracting method of resveratrol in particularly a kind of peanut sprout.
Background technology
Resveratrol is polyphenol compound, resveratrol (Res) chemical name 3,4,5- trihydroxies-talan,
Molecular formula C14H12O3,256~257 DEG C of fusing point.It is main in plant to exist in polydatin (PDT) form, mainly
From grape（Red wine）, giant knotweed, peanut, the plant such as mulberry fruit.
Resveratrol is a kind of biological very strong natural polyphenol class material, also known as resvertrol, is that the chemistry of tumour is pre-
Anti- dose, be also to reduction platelet aggregation, prevention and treatment atherosclerosis, the chemopreventive agent of cardiovascular and cerebrovascular disease.In vain
The experimental study of veratryl alcohol is had confirmed with the beneficial effect to angiocardiopathy and cancer.Blood fat can be reduced simultaneously to come of age
The incidence of disease of worry, special protection function is played to cardiovascular system；Adjust the ratio of low-density lipoprotein (LDL)；Reduce blood small
Plate aggegation etc. is acted on；Anti-aging；
Because resveratrol has a variety of biological and pharmacological activity, it is set to be widely used in food, medicine, health products, change
The fields such as cosmetic.Resveratrol has excellent pharmacological activity and healthcare function its market demand very big and increased severely with day, existing big
Countries and area all develop resveratrol and its product.The U.S. is using resveratrol as dietary supplements, and Japan is
Using from the resveratrol of plant extract as food additives, it is beautiful that lipid-loweringing has been made in the plant extracts containing resveratrol by China
The natural health care of appearance.
How substantial amounts of extraction and large-scale production resveratrol are always target that researcher is constantly pursued, tradition
Method be mostly that resveratrol is extracted from giant knotweed, but giant knotweed growth cycle is long, typically to grow containing for 3 years resveratrols
Amount can just reach harvesting amount, and the high giant knotweed of content is generally wild giant knotweed, and with a large amount of excavations of wild giant knotweed, giant knotweed resource is big
Amount is reduced, and a large amount of extractions as giant knotweed resveratrol propose problem.
Everybody has been accepted as grape, grape pip, grape wine（Red grape）In contain resveratrol, traditional habit is drunk the state of white wine
People is in the receiving to health theory, also begins to drink grape wine；Research has shown that：The grape wine for drinking health receiving amount rises not
To effect；Extract that resveratrol is good does not produce on a large scale with Grape Skin, grape pip.
Research shows that peanut red coat also contains substantial amounts of resveratrol, but peanut red coat collects difficult, studies in theory
Feasible but often because of raw material in actually large-scale production scarcity causes actual production not implement.
In Pennsylvania State Univ-Univ Park USA professor Pei Ni and Taiwan Chiayi college professor Qiu Yi sources etc. research, peanut
Containing resveratrol, its content is the thirtyfold of grape.Resveratrol content generally only hundred a ten thousandths in grape, and spend
Resveratrol content in life is but up to 30/1000000ths.Qiu Yiyuan points out, white black false hellebore of the general red wine containing about one to two ppm
Alcohol, but peanut sprout contains 30 ppm, after particular form is cultivated, can more lift to 300 to 500 ppm, peanut hair
After bud, its Resveratrol content increases sharply, and assay shows, the Resveratrol content in peanut sprout is the 100 of shelled peanut
Times, it is also be higher by Resveratrol content in grape 100 times；Resveratrol is extracted in peanut vernalization, can really realize resveratrol
Large-scale production.
The content of the invention
It is abundant it is an object of the invention to provide a kind of raw material sources based on above reason, and Resveratrol content is high, extracts
Simplicity, facilitates feasible, is easy to the extracting method of the resveratrol of large-scale production：Realizing the technical scheme of above-mentioned purpose is：
The extracting method of resveratrol, is comprised the steps of in a kind of peanut sprout：
Step 1: peanut seed is handled：
Select that grain big, full seed, fresh color, epidermis be smooth, the consistent high-quality of shape peanut seed, it is new by what is chosen
Fresh peanut is put into container and added water, and water is peanut weight 40%~50%, in 20~25 DEG C of warm water, immersion peanut kind 12
Hour, seed soaking loads 25 DEG C of constant temperature germinating boxs after finishing, drench the warm water of 25 DEG C within 4 hours, is sprouted after first time bubble bud 3d,
The seed for having urged bud is once selected, the seed not germinateed is removed, the seed germinateed secondary vernalization is subjected to, at 25 DEG C
In constant temperature germinating box, 8d peanut sprouts grow to 7 centimetres, and peanut sprout pushes away scarlet, and vernalization is completed when leaf bud exposes bud valve；Now spend
Middle Resveratrol content of sprouting reaches peak；
Step 2: peanut sprout resveratrol extracts pre-treatment：
Peanut sprout obtained by step one is drained, quickly suddenly frozen peanut sprout after draining, solidification point is that -30 DEG C of jellies 4 are small
When, taking-up, which is put into 20~25 DEG C of warm water, thaws, and has thawed and has dried water with centrifuge；Repeat operation quick-frozen once；Pre-freeze
Peanut sprout afterwards is put into vacuum tank by dropping to pressure in window below three phase point by vacuum system, by heating system heat supply
To material, material moisture is set gradually to be evaporated to half-dried in weak shape；Step 3: the weak peanut sprout extruding after quick-frozen；
It will be in weak shape peanut sprout by hand or mechanical means extruding is standby in threadiness；
Step 4: the water extraction of peanut sprout fiber：
Peanut sprout fiber is put into 100 DEG C of hot water, decocted 35 minutes, the aqueous solution is filtered to obtain, aforesaid operations are repeated 3 times；
The filtrate merging of gained is concentrated in vacuo to 30% standby；
Step 5：Acid-catalyzed hydrolysis：
By 30% concentrate obtained by step 4, by volume proportion, for 8% watery hydrochloric acid, that 6 are hydrolyzed at 60 DEG C with 8 times of amount is small
When, cooled down after reaction, it is standby；
Step 6：Purifying：
Coolant after reaction is subjected to chromatography with the large pore resin absorption column anticipated：Macroporous absorbent resin layer
Analysis process includes：1st, macroporous absorbent resin is pre-processed：The processing carried out by product operation instruction method；2nd, raise up absorption：Will step
After the coolant suction filtration of rapid five gained, the large pore resin absorption column above anticipated carries out loading absorption 3, chromatographic elution：Carry
Take after liquid loading, alcohol 95 elution is tracked and detected with ripple layer chromatography, collect resveratrol section eluent
Step 7：By the resveratrol eluent being collected into, decolourized using charcoal absorption, then with spray drying legal system
Obtain resveratrol recrystallized.
In step one, peanut seed treatment temperature it is too high, though growth is fast, sprout is thin and delicate, easy aging；Temperature is too low, then
Growth is slow, time length easily rotten bud or cotyledon the first transaction of a day's business polypetalous, poor quality, and experimental study by contrast is obtained with the method for the present invention
Peanut sprout now Resveratrol content highest in peanut sprout, subsequent operation dialogue Li Lu alcohol extractings influence is small.
The moisture in peanut sprout is removed substantially in step 2, using freezing, and do not influence the white black false hellebore in peanut sprout
Alcohol composition；Influence is not resulted on follow-up operation.
The present invention is hydrolyzed with sour water again after being extracted with water, further by being eluted with alcohol, can not only be made white in raw material
Veratryl alcohol is sufficiently extracted, while the resveratrol glycoside in peanut sprout can also be made to be hydrolyzed to the aglycon of free state, is entered
One step increases the content of resveratrol, is resveratrol purity while the impurity in extract can also be removed by the present invention
Higher, quality is more stable.
Resveratrol raw material sources are extracted by this method to enrich, and are produced into local, workable, suitable extensive life
Production, while gained resveratrol purity height is extracted,
Specific implementation method
The content to invention is described in detail with reference to embodiments
The extracting method of resveratrol, is comprised the steps of in a kind of peanut sprout：
Step 1: peanut seed is handled：
Select that grain big, full seed, fresh color, epidermis be smooth, the consistent high-quality of shape peanut seed, it is new by what is chosen
Fresh peanut is put into container and added water, and water is peanut weight 40%~50%, in 20~25 DEG C of warm water, immersion peanut kind 12
Hour, seed soaking loads 25 DEG C of constant temperature germinating boxs after finishing, drench the warm water of 25 DEG C within 4 hours, is sprouted after first time bubble bud 3d,
The seed for having urged bud is once selected, the seed not germinateed is removed, the seed germinateed secondary vernalization is subjected to, at 25 DEG C
In constant temperature germinating box, 8d peanut sprouts grow to 7 centimetres, and peanut sprout pushes away scarlet, and vernalization is completed when leaf bud exposes bud valve；Now spend
Middle Resveratrol content of sprouting reaches peak；
Step 2: peanut sprout resveratrol extracts pre-treatment：
Peanut sprout obtained by step one is drained, quickly suddenly frozen peanut sprout after draining, solidification point is that -30 DEG C of jellies 4 are small
When, taking-up, which is put into 20~25 DEG C of warm water, thaws, and has thawed and has dried water with centrifuge；Repeat operation quick-frozen once；Pre-freeze
Peanut sprout afterwards is put into vacuum tank by dropping to pressure in window below three phase point by vacuum system, by heating system heat supply
To material, material moisture is gradually evaporated to half-dried in weak shape, the moisture in peanut sprout is removed substantially, and do not influence peanut
Resveratrol ingredient in bud；
Step 3: the weak peanut sprout extruding after quick-frozen：
It will be in weak shape peanut sprout by hand or mechanical means extruding is standby in threadiness；
Step 4: the water extraction of peanut sprout fiber：
Peanut sprout fiber is put into 100 DEG C of hot water, decocted 35 minutes, the aqueous solution is filtered to obtain, aforesaid operations are repeated 3 times；
The filtrate merging of gained is concentrated in vacuo to 30% standby；
Step 5：Acid-catalyzed hydrolysis：
By 30% concentrate obtained by step 4, by volume proportion, for 8% watery hydrochloric acid, that 6 are hydrolyzed at 60 DEG C with 8 times of amount is small
When, cooled down after reaction, it is standby；
Step 6：Purifying：
Coolant after reaction is subjected to chromatography with the large pore resin absorption column anticipated：Macroporous absorbent resin layer
Analysis process includes：1st, macroporous absorbent resin is pre-processed：The processing carried out by product operation instruction method；2nd, raise up absorption：Will step
After the coolant suction filtration of rapid five gained, the large pore resin absorption column above anticipated carries out loading absorption 3, chromatographic elution：Carry
Take after liquid loading, alcohol 95 elution is tracked and detected with ripple layer chromatography, collect resveratrol section eluent；
Step 7：By the resveratrol eluent being collected into, decolourized using charcoal absorption, then with spray drying legal system
Obtain resveratrol recrystallized.
Claims (1)
1. the extracting method of resveratrol in a kind of peanut sprout, it is characterised in that comprise the steps of：
Step 1: peanut seed is handled：
Select that grain big, full seed, fresh color, epidermis be smooth, the consistent high-quality of shape peanut seed, by the fresh flowers chosen
Raw be put into container adds water, and water is peanut weight 40%~50%, in 20~25 DEG C of warm water, and immersion peanut kind 12 is small
When, seed soaking loads 25 DEG C of constant temperature germinating boxs after finishing, drench the warm water of 25 DEG C within 4 hours, is sprouted after first time bubble bud 3d, will
Urge the seed of bud once to be selected, removed the seed not germinateed, the seed germinateed is subjected to secondary vernalization, in 25 DEG C of perseverances
In warm germinating box, 8d peanut sprouts grow to 7 centimetres, and peanut sprout pushes away scarlet, and vernalization is completed when leaf bud exposes bud valve；Now peanut
Resveratrol content reaches peak in bud；
Step 2: peanut sprout resveratrol extracts pre-treatment：
Peanut sprout obtained by step one is drained, quickly suddenly frozen peanut sprout after draining, solidification point is -30 DEG C and frozen 4 hours, takes
Go out to be put into 20~25 DEG C of warm water and thaw, thawed and dried water with centrifuge；Repeat operation quick-frozen once；Flower after pre-freeze
Sprout and be put into vacuum tank by dropping to pressure in window below three phase point by vacuum system, by heating system heat supply to thing
Material, makes material moisture gradually be evaporated to half-dried in weak shape, the moisture in peanut sprout is removed substantially, and do not influence in peanut sprout
Resveratrol ingredient；
Step 3: the weak peanut sprout extruding after quick-frozen：
It will be in weak shape peanut sprout by hand or mechanical means extruding is standby in threadiness；
Step 4: the water extraction of peanut sprout fiber：
Peanut sprout fiber is put into 100 DEG C of hot water, decocted 35 minutes, the aqueous solution is filtered to obtain, aforesaid operations are repeated 3 times；By institute
It is standby that the filtrate merging obtained is concentrated in vacuo to 30%；
Step 5: acid-catalyzed hydrolysis：
30% concentrate obtained by step 4 is hydrolyzed 6 hours for 8% watery hydrochloric acid by volume proportion with 8 times of amount at 60 DEG C,
Cooled down after reaction, it is standby；
Step 6: purifying：
Coolant after reaction is subjected to chromatography with the large pore resin absorption column anticipated：Macroporous adsorption resin chromatography mistake
Journey includes：1st, macroporous absorbent resin is pre-processed：The processing carried out by product operation instruction method；2nd, raise up absorption：By step 5
After the coolant suction filtration of gained, the large pore resin absorption column above anticipated carries out loading absorption 3, chromatographic elution：Extract solution
After loading, alcohol 95 elution is tracked and detected with ripple layer chromatography, collects resveratrol section eluent；
Step 7: by the resveratrol eluent being collected into, being decolourized using charcoal absorption, then it is made white with spray drying process
Veratryl alcohol is crystallized.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510393064.7A CN105016981B (en) | 2015-07-07 | 2015-07-07 | The extracting method of resveratrol in a kind of peanut sprout |
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| CN201510393064.7A CN105016981B (en) | 2015-07-07 | 2015-07-07 | The extracting method of resveratrol in a kind of peanut sprout |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2017137957A1 (en) * | 2016-02-11 | 2017-08-17 | Tripathi Vinaykumar | Colloidally stable resveratrol nanoparticles with improved bioavailability and half-life and synthesis thereof |
| CN106233873A (en) * | 2016-08-04 | 2016-12-21 | 巢湖市粮食协会 | A kind of method improving peanut seed germination percentage |
| WO2018042324A1 (en) * | 2016-08-29 | 2018-03-08 | Tripathi Vinaykumar | Modified resveratrol composition and use thereof |
| US11033514B2 (en) | 2016-08-29 | 2021-06-15 | Vinaykumar TRIPATHI | Modified resveratrol composition and use thereof |
| CN111296289B (en) * | 2020-03-12 | 2021-08-31 | 蚌埠学院 | Method for improving resveratrol content in peanut sprouts by cell suspension culture technology |
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| KR101403964B1 (en) * | 2012-08-29 | 2014-06-13 | 농업회사법인(주)장수채 | Extract method of peanut sprout increased resveratrol content |
| CN104003849B (en) * | 2013-09-17 | 2016-05-18 | 黄桂淑 | The extracting method of resveratrol in peanut sprout |
| CN103999748B (en) * | 2013-09-17 | 2016-02-10 | 黄桂淑 | The method of the peanut bud seedling of resveratrol is rich in a kind of acquisition |
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