CN109320576A - A kind of production method of high-content momordica grosvenori glycoside V - Google Patents
A kind of production method of high-content momordica grosvenori glycoside V Download PDFInfo
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- CN109320576A CN109320576A CN201811250821.5A CN201811250821A CN109320576A CN 109320576 A CN109320576 A CN 109320576A CN 201811250821 A CN201811250821 A CN 201811250821A CN 109320576 A CN109320576 A CN 109320576A
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- momordica grosvenori
- grosvenori glycoside
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- 241001409321 Siraitia grosvenorii Species 0.000 title claims abstract description 55
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 title claims abstract description 55
- 229930182470 glycoside Natural products 0.000 title claims abstract description 39
- 150000002338 glycosides Chemical class 0.000 title claims abstract description 39
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 12
- 238000010438 heat treatment Methods 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 238000007710 freezing Methods 0.000 claims abstract description 9
- 230000008014 freezing Effects 0.000 claims abstract description 9
- 230000008569 process Effects 0.000 claims abstract description 9
- 230000001052 transient effect Effects 0.000 claims abstract description 7
- 238000000605 extraction Methods 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 39
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 31
- 239000007788 liquid Substances 0.000 claims description 23
- 238000000926 separation method Methods 0.000 claims description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 235000019441 ethanol Nutrition 0.000 claims description 11
- 239000012528 membrane Substances 0.000 claims description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 8
- 239000011347 resin Substances 0.000 claims description 7
- 229920005989 resin Polymers 0.000 claims description 7
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 6
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- 238000010521 absorption reaction Methods 0.000 claims description 6
- 239000000919 ceramic Substances 0.000 claims description 6
- 238000004587 chromatography analysis Methods 0.000 claims description 6
- 238000002203 pretreatment Methods 0.000 claims description 6
- 239000003957 anion exchange resin Substances 0.000 claims description 5
- 239000003729 cation exchange resin Substances 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 239000000284 extract Substances 0.000 claims description 5
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 235000013399 edible fruits Nutrition 0.000 claims description 2
- 239000003480 eluent Substances 0.000 claims description 2
- 239000000945 filler Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000003960 organic solvent Substances 0.000 claims description 2
- 239000000741 silica gel Substances 0.000 claims description 2
- 229910002027 silica gel Inorganic materials 0.000 claims description 2
- 239000003456 ion exchange resin Substances 0.000 claims 1
- 229920003303 ion-exchange polymer Polymers 0.000 claims 1
- 235000013372 meat Nutrition 0.000 claims 1
- 238000007781 pre-processing Methods 0.000 claims 1
- 238000009776 industrial production Methods 0.000 abstract description 2
- 239000000419 plant extract Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 description 9
- GHBNZZJYBXQAHG-KUVSNLSMSA-N (2r,3r,4s,5s,6r)-2-[[(2r,3s,4s,5r,6r)-6-[[(3s,8s,9r,10r,11r,13r,14s,17r)-17-[(2r,5r)-5-[(2s,3r,4s,5s,6r)-4,5-dihydroxy-3-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-[[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@H](CC[C@@H](C)[C@@H]1[C@]2(C[C@@H](O)[C@@]3(C)[C@H]4C(C([C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]6[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O6)O)O5)O)CC4)(C)C)=CC[C@H]3[C@]2(C)CC1)C)C(C)(C)O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GHBNZZJYBXQAHG-KUVSNLSMSA-N 0.000 description 4
- TVJXHJAWHUMLLG-UHFFFAOYSA-N mogroside V Natural products CC(CCC(OC1OC(COC2OC(CO)C(O)C(O)C2OC3OC(CO)C(O)C(O)C3O)C(O)C(O)C1O)C(C)(C)O)C4CCC5(C)C6CC=C7C(CCC(OC8OC(COC9OC(CO)C(O)C(O)C9O)C(O)C(O)C8O)C7(C)C)C6(C)C(O)CC45C TVJXHJAWHUMLLG-UHFFFAOYSA-N 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229930189775 mogroside Natural products 0.000 description 3
- 206010062717 Increased upper airway secretion Diseases 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 235000003599 food sweetener Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 235000015110 jellies Nutrition 0.000 description 2
- 239000008274 jelly Substances 0.000 description 2
- 208000026435 phlegm Diseases 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000003765 sweetening agent Substances 0.000 description 2
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 description 1
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 208000007882 Gastritis Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 201000008197 Laryngitis Diseases 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 235000009815 Momordica Nutrition 0.000 description 1
- 241000218984 Momordica Species 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 238000010936 aqueous wash Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- -1 cucurbitane triterpene glycosides compound Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 210000003800 pharynx Anatomy 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 206010044008 tonsillitis Diseases 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J17/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, having an oxygen-containing hetero ring not condensed with the cyclopenta(a)hydrophenanthrene skeleton
- C07J17/005—Glycosides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Saccharide Compounds (AREA)
- Steroid Compounds (AREA)
Abstract
The invention discloses a kind of production methods of high-content momordica grosvenori glycoside V, belong to natural plant extracts production field, to carry out the Siraitia grosvenorii of TRANSIENT HIGH TEMPERATURE heat treatment immediately as raw material after freezing.The present invention has many advantages, such as that the at low cost, high income of extraction, product purity is high, water consumption is small, simple process, large-scale continuous industrial production can be achieved.
Description
Technical field
The invention belongs to extracted form natural plant fields, and in particular to a kind of industrialized preparing process of food ingredient.
Background technique
Siraitia grosvenorii (Siraitia grosvenorii) is a kind of perennial liana of Curcurbitaceae of dual-purpose of drug and food.Chinese medicine
Lung heat phlegm-fire cough, pharynx are cured mainly containing medicinal ingredients such as mogroside, a variety of amino acid and vitamins with its fruit medicine
Laryngitis, tonsillitis, acute gastritis, constipation etc..Mogroside in Siraitia grosvenorii belongs to cucurbitane triterpene glycosides compound, main
To include Momordia grosvenori aglycone IV, momordica grosvenori glycoside V and Simon glycosides I etc., wherein with Mogroside V content highest, account for about fresh fruit weigh 0.4~
0.5%, sugariness is equivalent to 300 times of sucrose, but it has the characteristics that not induce saprodontia, does not cause blood glucose rise, is ordinary people
Group and obesity, hypertension, diabetic excellent sweetener.Clinical test proves, mogroside also have eliminating the phlegm,
Antibechic, removes the plurality of health care functions such as free radical and anti-cancer at strengthen immunity.With the improvement of living standards and people are to strong
The raising of health degree of concern, momordica glycoside V are also constantly increasing as a kind of domestic demand amount of excellent sweetener.
Currently, the extracting method of momordica grosvenori glycoside V is more in the prior art, but have that complex process, yield are low, product is pure
Spend many defects (such as Chinese patent application CN201410190403.7) such as low, water consumption is big.
Therefore it provides a kind of industrial metaplasia for the momordica grosvenori glycoside V that simple process, high income, product purity are high, water consumption is small
Production method becomes the technical issues of there is an urgent need to those skilled in the art's solutions.
Summary of the invention
Through long term test, applicant develops a kind of production method of high-content momordica grosvenori glycoside V, at low cost, receipts
Rate is high, product purity is high, water consumption is small, simple process, the advantages that large-scale continuous industrial production can be achieved.
Specifically, the present invention provides a kind of production methods of high-content momordica grosvenori glycoside V, including by raw material Siraitia grosvenorii through cold
The pre-treatment step of TRANSIENT HIGH TEMPERATURE heating is carried out after jelly immediately.
More specifically, including the following steps: the present invention provides a kind of production method of high-content momordica grosvenori glycoside V
1) pre-process: by raw material Siraitia grosvenorii it is chilled after carry out TRANSIENT HIGH TEMPERATURE heating immediately；
2) it extracts: will be removed the peel by pretreated Siraitia grosvenorii, pulp crushing adds water to carry out circulated in countercurrent extraction, must extract
Liquid；
3) filter: extracting solution is centrifuged, and the supernatant being centrifuged obtains filtered fluid through ceramic membrane filter；
4) absorption and parsing: filtered fluid is parsed after macroporous resin column is adsorbed with ethanol solution, obtains desorbed solution；
5) decolourize: desorbed solution passes sequentially through cation exchange resin, anion exchange resin, collects column liquid；
6) it concentration and drying: crosses column liquid and is concentrated, is dry, obtain momordica grosvenori glycoside V.
Wherein, content >=50% of the momordica grosvenori glycoside V.
A further improvement of the present invention is that the column liquid of crossing is handled through continuous chromatography separation system, refined liquid is obtained；Essence
Liquid processed is concentrated, is dry, obtains momordica grosvenori glycoside V；Wherein, content >=98% of the momordica grosvenori glycoside V.The wherein continuous chromatography
The filler of separation system is selected from one of C30, C18, C8, C4 or silica gel, eluent be water, selected from one of ethyl alcohol, acetonitrile, methanol or
The organic solvent of its mixture or its aqueous solution.
The pre-treatment step are as follows: after freezing raw material Siraitia grosvenorii 1~2 hour under the conditions of -25 DEG C~-10 DEG C, stand
Heated 5~50 seconds, preferably 10~40 seconds, more preferable 20~30 seconds under the conditions of 105 DEG C~120 DEG C.
Wherein, the preferably new fresh fructus momordicae of the raw material Siraitia grosvenorii of the step 1).
Wherein, when the pulp crushing of the step 2), seed is not destroyed, and is preferably maintained in good working condition；Circulated in countercurrent is extracted
When, the mass ratio of pulp and water is 1:2.5~6, and Extracting temperature is 50~90 DEG C.
Wherein, the step 3) carries out centrifugally operated using decanter centrifuge or decanter centrifuge series connection disk plate centrifuge,
The separation factor of the decanter centrifuge is 2500~6000, and the separation factor of the disk plate centrifuge is 8000~30000；Institute
The membrane tube aperture for stating ceramic membrane is 50~200nm.
Wherein, the macroreticular resin model of the step 4) is selected from one of T-28, HDP-100 or DA201-H；Described in wherein
The mass concentration of ethanol solution is 55~70%, and volume is 1.5~2.5BV；Prewashing is carried out before the ethanol solution parsing, first
The aqueous slkali prewashing for being 2~6 ‰ with the mass concentration of 1~3BV, then it is pre- with the acid solution that the mass concentration of 1~3BV is 2~6 ‰
It washes, it is neutral for being finally washed till column liquid with the pure water of 2~4BV.Wherein the aqueous slkali is sodium hydroxide solution or hydroxide
Potassium solution, acid solution are hydrochloric acid solution or sulfuric acid solution.
Wherein, the model 001 × 16 or 001 × 7 of the cation exchange resin of the step 5), the anion exchange
The model of resin is selected from one of 700B, D941, T-5 or D900.
The present invention has by the way that raw material Siraitia grosvenorii to be carried out to the pre-treatment step of TRANSIENT HIGH TEMPERATURE heat treatment immediately after chilled
Improve to effect the content and yield of momordica grosvenori glycoside V in target product.
According to the needs of target product purity, the present invention can produce Mogroside V content >=50% and >=98% two kind not
The momordica grosvenori glycoside V product of same specification.That is, column liquid is directly concentrated dry if Mogroside V content >=50% of target product
Dry, yield is up to 95% or more；If Mogroside V content >=98% of target product, by column liquid through continuous chromatography
It after separation, then is concentrated and dried, yield is up to 90% or more.
Specific embodiment
A specific embodiment of the invention is only to be explained further and illustrate the present invention, supplementary material used in the examples,
Equipment etc. is commercially available.
Embodiment 1
1) it pre-processes: new fresh fructus momordicae being placed in -25 DEG C of cryogenic freezing case, after freezing 1 hour, immediately with 105 DEG C
Steam heat 20 seconds；
2) it extracts: will be removed the peel by pretreated Siraitia grosvenorii, pulp quality 2.5 is added in pulp crushing (seed keeps complete)
Water again carries out circulated in countercurrent extraction, and Extracting temperature is 50 DEG C, obtains extracting solution；
3) filter: after the decanter centrifuge centrifugation that separation factor is 3000, supernatant is extracting solution through membrane tube aperture
The ceramic membrane filter of 50nm, obtains filtered fluid；
4) absorption and parsing: filtered fluid is first 2 ‰ with the mass concentration of 1BV after the absorption of DA201-H macroporous resin column
Sodium hydroxide solution prewashing, then the hydrochloric acid solution prewashing for being 2 ‰ with the mass concentration of 1BV, were then washed till column with the pure water of 2BV
Liquid is neutrality, is finally parsed with the ethanol solution that the mass concentration of 1.5BV is 55%, obtains desorbed solution；
5) decolourize: desorbed solution first passes through 001 × 16 cation exchange resin, then is carried out by T-5 anion exchange resin
Processing, collected column liquid；
6) it concentration and drying: crosses column liquid and is concentrated into solid content 35%, then be spray-dried, obtain momordica grosvenori glycoside V.
Embodiment 2
1) it pre-processes: new fresh fructus momordicae being placed in -10 DEG C of cryogenic freezing case, after freezing 1.5 hours, immediately with 120
DEG C steam heat 30 seconds；
2) it extracts: will be removed the peel by pretreated Siraitia grosvenorii, pulp crushing (seed keeps complete) is added 6 times of pulp quality
Water carry out circulated in countercurrent extraction, Extracting temperature be 90 DEG C, obtain extracting solution；
3) filter: extracting solution is 8500 after the decanter centrifuge centrifugation that separation factor is 3500, then through separation factor
Disk plate centrifuge centrifugation, supernatant through membrane tube aperture be 200nm ceramic membrane filter, obtain filtered fluid；
4) absorption and parsing: filtered fluid is after the absorption of T-28 macroporous resin column, the hydrogen for being first 6 ‰ with the mass concentration of 3BV
Sodium hydroxide solution prewashing, then the hydrochloric acid solution prewashing for being 6 ‰ with the mass concentration of 3BV, were then washed till column liquid with the pure water of 4BV
For neutrality, is finally parsed with the ethanol solution that the mass concentration of 1.5BV is 60%, obtain desorbed solution；
5) decolourize: desorbed solution first passes through 001 × 7 cation exchange resin, then is carried out by D900 anion exchange resin
Processing, collected column liquid；
6) it refines: the continuous chromatography separation system that column liquid is C18 through separating column packing is crossed, with 70% ethanol aqueous wash
It is de-, obtain refined liquid；
7) concentration and drying: refined liquid is concentrated into solid content 20%, and spray drying obtains momordica grosvenori glycoside V.
Embodiment 3~4
The preparation method of embodiment 3~4 is similar to Example 2, and difference is only that specific technological parameter is different and (is detailed in down
Table, wherein " amount of water " is the multiple of raw material Siraitia grosvenorii pulp quality).
Reference examples 1~4
The preparation method of reference examples 1~4 is similar with Examples 1 to 4 respectively, and difference is only that: reference examples 1,2 are not using cold
The pre-treatment step of TRANSIENT HIGH TEMPERATURE heating is carried out after jelly immediately；The cryogenic temperature of reference examples 3 is -30 DEG C, and cooling time is 1 small
When, high-temperature heating temperature is 130 DEG C, and heating time is 30 seconds；The cryogenic temperature of reference examples 4 is -5 DEG C, and cooling time is 3 small
When, high-temperature heating temperature is 110 DEG C, and heating time is 60 seconds.
Test example
Momordica grosvenori glycoside V prepared by Example 1~4, reference examples 1~4, (GB1886.77-2016) is examined according to national standards
Survey the content of momordica grosvenori glycoside V, and calculated yield according to the following formula: (momordica grosvenori glycoside V in quality/Siraitia grosvenorii of momordica grosvenori glycoside V in product
Quality) × 100%, it is as a result as follows:
| Content | Yield | |
| Embodiment 1 | 55.0% | 96% |
| Embodiment 2 | 98.5% | 91% |
| Embodiment 3 | 98.0% | 90% |
| Embodiment 4 | 98.2% | 91% |
| Reference examples 1 | 47.2% | 76% |
| Reference examples 2 | 92.3% | 85% |
| Reference examples 3 | 94.3% | 87% |
| Reference examples 4 | 93.5% | 84% |
As it can be seen that the content and yield of the momordica grosvenori glycoside V (such as Examples 1 to 2 2) of two specifications prepared by the present invention are obvious
Better than the reference examples 1~2 for not using pre-treatment step accordingly；In particular, momordica grosvenori glycoside V (such as embodiment 3 prepared by the present invention
~4) content and yield is also significantly better than that corresponding freezing, heating technique parameter value are claimed outside range in the present invention
Reference examples 3~4.
Claims (10)
1. a kind of production method of high-content momordica grosvenori glycoside V, including add TRANSIENT HIGH TEMPERATURE is carried out immediately after the freezing of raw material Siraitia grosvenorii
The pre-treatment step of heat.
2. the production method of high-content momordica grosvenori glycoside V according to claim 1, includes the following steps:
1) pre-process: by raw material Siraitia grosvenorii it is chilled after carry out TRANSIENT HIGH TEMPERATURE heating immediately；
2) it extracts: will be removed the peel by pretreated Siraitia grosvenorii, pulp crushing adds water to carry out circulated in countercurrent extraction, obtains extracting solution；
3) filter: extracting solution is centrifuged, and the supernatant being centrifuged obtains filtered fluid through ceramic membrane filter；
4) absorption and parsing: filtered fluid is parsed after macroporous resin column is adsorbed with ethanol solution, obtains desorbed solution；
5) decolourize: desorbed solution passes sequentially through cation exchange resin, anion exchange resin, collects column liquid；
6) it concentration and drying: crosses column liquid and is concentrated, is dry, obtain momordica grosvenori glycoside V；
Wherein, content >=50% of the momordica grosvenori glycoside V.
3. the production method of high-content momordica grosvenori glycoside V according to claim 2, wherein described crosses column liquid through continuous chromatography
Separation system processing, obtains refined liquid；Refined liquid concentration, drying, obtain momordica grosvenori glycoside V；Wherein, the content of the momordica grosvenori glycoside V
≥98%。
4. the production method of high-content momordica grosvenori glycoside V according to claim 3, wherein the continuous chromatography separation system
Filler is selected from one of C30, C18, C8, C4 or silica gel, and eluent is water, selected from or mixtures thereof one of ethyl alcohol, acetonitrile, methanol
Organic solvent or its aqueous solution.
5. the production method of high-content momordica grosvenori glycoside V according to any one of claims 1 to 4, wherein the step 1) is pre-
Processing step are as follows: after freezing raw material Siraitia grosvenorii 1 ~ 2 hour under the conditions of -25 DEG C~-10 DEG C, immediately in 105 DEG C~120 DEG C items
It is heated 5 ~ 50 seconds under part.
6. according to the production method of any high-content momordica grosvenori glycoside V of claim 2~4, wherein the fruit of the step 2
When meat is crushed, seed keeps complete；When circulated in countercurrent is extracted, the mass ratio of pulp and water is 1:2.5~6, Extracting temperature is 50~
90℃。
7. according to the production method of any high-content momordica grosvenori glycoside V of claim 2~4, wherein the step 3) uses
Decanter centrifuge or decanter centrifuge series connection disk plate centrifuge carry out centrifugally operated, and the separation factor of the decanter centrifuge is
2500~6000, the separation factor of the disk plate centrifuge is 8000~30000；The membrane tube aperture of the ceramic membrane be 50~
200nm。
8. according to the production method of any high-content momordica grosvenori glycoside V of claim 2~4, wherein the step 4) is big
Hole resin model is selected from one of T-28, HDP-100 or DA201-H；Wherein the mass concentration of the ethanol solution be 55~
70%, volume is 1.5~2.5BV；Carry out prewashing before the described ethanol solution parsing, first with the mass concentration of 1~3BV for 2~
6 ‰ aqueous slkali prewashing, then the acid solution prewashing for being 2~6 ‰ with the mass concentration of 1~3BV, are finally washed with the pure water of 2~4BV
It is neutrality to column liquid is crossed.
9. the production method of high-content momordica grosvenori glycoside V according to claim 8, wherein the aqueous slkali is sodium hydroxide
Solution or potassium hydroxide solution, the acid solution are hydrochloric acid solution or sulfuric acid solution.
10. according to the production method of any high-content momordica grosvenori glycoside V of claim 2~4, wherein the sun of the step 5)
The model of the model 001 × 16 or 001 × 7 of ion exchange resin, the anion exchange resin is selected from 700B, D941, T-5
Or one of D900.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110669095A (en) * | 2019-10-14 | 2020-01-10 | 广西壮族自治区科学技术情报研究所 | Extraction method of high-purity mogroside V |
| CN111978365A (en) * | 2020-09-10 | 2020-11-24 | 上海诺德生物实业有限公司 | Preparation method of mogroside V-containing mogroside |
| CN116941765A (en) * | 2023-07-28 | 2023-10-27 | 湖南华诚生物资源股份有限公司 | An improved co-production method of pesticide-free mogroside composition |
| WO2025000615A1 (en) * | 2023-06-28 | 2025-01-02 | 桂林吉福思罗汉果生物技术股份有限公司 | Sweetness composition and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103923152A (en) * | 2014-05-08 | 2014-07-16 | 济南汉定生物工程有限公司 | Method for extracting mogroside V |
| CN104312921A (en) * | 2014-09-28 | 2015-01-28 | 广州英佩尔电子科技有限公司 | Comprehensive micro-molecule wall breaking method used for fresh food and drug materials |
| CN108276465A (en) * | 2017-03-14 | 2018-07-13 | 桂林莱茵生物科技股份有限公司 | A method of isolating and purifying mogroside V with subcritical water desorption techniques |
-
2018
- 2018-10-25 CN CN201811250821.5A patent/CN109320576B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103923152A (en) * | 2014-05-08 | 2014-07-16 | 济南汉定生物工程有限公司 | Method for extracting mogroside V |
| CN104312921A (en) * | 2014-09-28 | 2015-01-28 | 广州英佩尔电子科技有限公司 | Comprehensive micro-molecule wall breaking method used for fresh food and drug materials |
| CN108276465A (en) * | 2017-03-14 | 2018-07-13 | 桂林莱茵生物科技股份有限公司 | A method of isolating and purifying mogroside V with subcritical water desorption techniques |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110669095A (en) * | 2019-10-14 | 2020-01-10 | 广西壮族自治区科学技术情报研究所 | Extraction method of high-purity mogroside V |
| CN110669095B (en) * | 2019-10-14 | 2022-05-27 | 广西壮族自治区科学技术情报研究所 | Extraction method of high-purity mogroside V |
| CN111978365A (en) * | 2020-09-10 | 2020-11-24 | 上海诺德生物实业有限公司 | Preparation method of mogroside V-containing mogroside |
| WO2025000615A1 (en) * | 2023-06-28 | 2025-01-02 | 桂林吉福思罗汉果生物技术股份有限公司 | Sweetness composition and preparation method thereof |
| CN116941765A (en) * | 2023-07-28 | 2023-10-27 | 湖南华诚生物资源股份有限公司 | An improved co-production method of pesticide-free mogroside composition |
| CN116941765B (en) * | 2023-07-28 | 2025-02-14 | 湖南华诚生物资源股份有限公司 | An improved method for co-producing a pesticide-free mogroside composition |
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