CN106632528A - Preparation method of passion fruit oligosaccharide - Google Patents
Preparation method of passion fruit oligosaccharide Download PDFInfo
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- CN106632528A CN106632528A CN201611226999.7A CN201611226999A CN106632528A CN 106632528 A CN106632528 A CN 106632528A CN 201611226999 A CN201611226999 A CN 201611226999A CN 106632528 A CN106632528 A CN 106632528A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/04—Disaccharides
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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Abstract
The invention relates to a preparation method of passion fruit oligosaccharide, belonging to the technical field of extraction of oligosaccharide. The preparation method comprises six steps: selection of fruits, peeling, pulping, microwave extraction of oligosaccharide in super ionic liquid, suction filtration separation and freeze concentration, concretely, selecting passion fruits with brown peel, peeling the passion fruits by hands, reserving yellow pulp in the middle, mixing the pulp with water in weight ratio of 1:10-1:20, pulping for 20-60s in a pulping machine, adding 1-hexyl-2-methylimidazole bromide as an extraction solution to the passion fruit pulp in weight ratio of (1:1)-(1:5), extracting at room temperature for 1-5h; adding 2-5 times of absolute ethyl alcohol to the passion fruit pulp after extraction, carrying out suction filtration separation at room temperature, and carrying out vacuum rotary evaporation at 30-40 DEG C; freeze drying the residue after evaporation at -30 to -10 DEG C for 12-24h, pulverizing and preserving. The preparation method has high extraction rate and can effectively avoid damage of the oligosaccharide component due to temperature, and the prepared oligosaccharide has high preservation activity.
Description
Technical field
The present invention relates to a kind of preparation method of compound sugar, and in particular to a kind of preparation method of passion fruit compound sugar, category
In compound sugar extractive technique field.
Background technology
Passion fruit, also known as passion fruit, are the herbaceous species plants of Passifloraceae Passiflora, are about 6 meters；Stem has slice
Line, without hair；5 pieces of petal, it is isometric with sepal；Base portion light green, middle part purple, top white, berry ovoid, 3～4 lis of diameter
Rice, without hair, purple when ripe；Seed is most, avette.June at florescence, fruiting period November.Natural pure, strong sweet and refreshing, sweet and sour taste, it is raw
Quench the thirst in Tianjin；Refresh the mind, relax bowel, prevent colorectal cancer；Reinforcing kidney and enhancing fitness, toxin-expelling and face nourishing；Relieving alcoholism and protecting liver, dispelling fatigue；To height
It is powerful that blood pressure, hyperglycaemia reduce cholesterol；Canker sore, periodontitis, sphagitis are powerful；It is active to colitis, enterogastritis
Effect；Adjust blood sugar, help prevention diabetes；Form strong bone, osteoporosis of releiving；Improve body immunity, prevention disease.
Ionic liquid（Or title ionic liquid）The liquid being composed entirely of ions, the such as KCI under high temperature are referred to, KOH is in
Liquid condition, now they are exactly ionic liquid.The material being made up of ion being in a liquid state at a temperature of room temperature or near room temperature,
Referred to as ionic liquid at room temperature, room temperature molten salt（Ionic liquid at room temperature is often accompanied by the presence of hydrogen bond, is defined as room temperature molten salt a little
Reluctantly）, organic ion liquid etc., unified title is there is no at present, but tend to abbreviation ionic liquid.In ionic compound,
Active force between zwitterion is Coulomb force, and its size is relevant with the amount of charge of zwitterion and radius, and ionic radius is got over
Greatly, the active force between them is less, and the fusing point of this ionic compound is lower.The zwitterion body of some ionic compounds
Product is very big, loosely organized, causes the active force between them relatively low, so that the close room temperature of fusing point.
Compound sugar integrates nutrition, health care, dietotherapy, is widely used in food, health products, beverage, medicine, feed addition
The fields such as agent.It is the new type functional sugar source for substituting sucrose, is Twenty-First Century " future type " a new generation functional food.
It is a kind of new product with generally applicable scope and application prospect, it is rather popular in the world in recent years.The U.S., Japan, Europe
Etc. the ground development and application that has large-scale production, China's compound sugar arise from the mid-90, develop in recent years swift and violent.With work
Change in enteron aisle Bifidobacterium and promote the function of its growth and breeding.Bifidobacterium is the beneficial bacterium in human body intestinal canal, its bacterium number meeting
Gradually decrease with the increase at age.In enteron aisle Bifidobacterium number into one of health whether index weighed.With
The antibiotic for medical science applied fast development, wide spectrum and strength is widely used in treating various diseases, makes in human body intestinal canal just
Normal colony balance is subject to different degrees of destruction.Thus, the beneficial bacterium quantity purposefully increased in enteron aisle just seems very
It is necessary.Intake Bifidobacterium product is no doubt easy to be reliable, but this kind of product is all limited from producing to selling by many conditions,
And it is then more practical to promote Bifidobacterium in enteron aisle to accrue by taking in functional oligose.
Chinese patent, application number：201310423541.0, a kind of preparation method of lycium barbarum oligosaccharide, using alcohol reflux
Extraction process prepares lycium barbarum oligosaccharide, and the compound sugar Jing macroporous absorbent resins for obtaining decolourize, and can effectively remove depigmentaton, improves oligomeric
Sugared purity.But the method adopts macroporous resin purification compound sugar, its activity is affected, and reduce its recovery rate, therefore developed
A kind of efficient compound sugar extracting method tool is of great significance.
The content of the invention
It is an object of the invention to provide a kind of preparation method of passion fruit compound sugar, preparation method recovery rate height, can have
Effect avoids destruction of the temperature to compound sugar composition, and the compound sugar for preparing preserves activity height.
To realize the purpose of foregoing invention, the technical scheme that the present invention takes is as follows：A kind of preparation of passion fruit compound sugar
Method, comprises the following steps：
（1）Choose fruit：The tan passion fruit of passion fruit skin is chosen, corrupt, rotten fruit is removed；
（2）Peeling, stripping seed：Staff peels off passion fruit appearance, retains middle yellow pulp；
（3）Beating：By passion fruit pulp and water by weight 1:10-1:20 mixing, are placed in beater and are beaten 20-60s；
（4）Super-ionic liquid Microwave Extraction compound sugar：Using bromination 1- hexyl -2-methylimidazole as extraction solution, passion fruit meat
Slurry is 1 with bromination 1- hexyl -2-methylimidazole by weight:1-1:5, extract 1-5h under room temperature；
（5）Suction filtration is separated：2-5 times of absolute ethyl alcohol is added in passion fruit slurry after extraction, at room temperature suction filtration is separated, and in
Rotary evaporation in vacuo at 30-40 DEG C；
（6）Freeze concentration：Slag after evaporation freezes 12-24h at being placed in -30 DEG C to -10 DEG C, preserves after crushing.
The present invention is relative to the beneficial effect of prior art：
The present invention adopts ionic liquid extract compound sugar, and high temperature can be avoided to extract the destruction to passion fruit Active components, extracts effect
Rate is high, and the compound sugar of preparation preserves activity height, and ionic liquid is reusable, greatlys save extraction cost.
Specific embodiment
The present invention is described in further details below by example, these examples are only used for illustrating the present invention, do not limit
The scope of the present invention processed.
Embodiment 1
A kind of preparation method of passion fruit compound sugar, comprises the following steps：
（1）Choose fruit：The tan passion fruit of passion fruit skin is chosen, corrupt, rotten fruit is removed；
（2）Peeling, stripping seed：Staff peels off passion fruit appearance, retains middle yellow pulp；
（3）Beating：By passion fruit pulp and water by weight 1:10 mixing, are placed in beater and are beaten 20s；
（4）Super-ionic liquid Microwave Extraction compound sugar：Using bromination 1- hexyl -2-methylimidazole as extraction solution, passion fruit meat
Slurry is 1 with bromination 1- hexyl -2-methylimidazole by weight:1, extract 1h under room temperature；
（5）Suction filtration is separated：2 times of absolute ethyl alcohol is added in passion fruit slurry after extraction, at room temperature suction filtration is separated, and in 30 DEG C
Lower rotary evaporation in vacuo；
（6）Freeze concentration：Slag after evaporation freezes 12h at being placed in -30 DEG C, preserves after crushing.
Embodiment 2
A kind of preparation method of passion fruit compound sugar, comprises the following steps：
（1）Choose fruit：The tan passion fruit of passion fruit skin is chosen, corrupt, rotten fruit is removed；
（2）Peeling, stripping seed：Staff peels off passion fruit appearance, retains middle yellow pulp；
（3）Beating：By passion fruit pulp and water by weight 1:12 mixing, are placed in beater and are beaten 30s；
（4）Super-ionic liquid Microwave Extraction compound sugar：Using bromination 1- hexyl -2-methylimidazole as extraction solution, passion fruit meat
Slurry is 1 with bromination 1- hexyl -2-methylimidazole by weight:2, extract 2h under room temperature；
（5）Suction filtration is separated：3 times of absolute ethyl alcohol is added in passion fruit slurry after extraction, at room temperature suction filtration is separated, and in 32 DEG C
Lower rotary evaporation in vacuo；
（6）Freeze concentration：Slag after evaporation freezes 14h at being placed in -25 DEG C, preserves after crushing.
Embodiment 3
A kind of preparation method of passion fruit compound sugar, comprises the following steps：
（1）Choose fruit：The tan passion fruit of passion fruit skin is chosen, corrupt, rotten fruit is removed；
（2）Peeling, stripping seed：Staff peels off passion fruit appearance, retains middle yellow pulp；
（3）Beating：By passion fruit pulp and water by weight 1:14 mixing, are placed in beater and are beaten 40s；
（4）Super-ionic liquid Microwave Extraction compound sugar：Using bromination 1- hexyl -2-methylimidazole as extraction solution, passion fruit meat
Slurry is 1 with bromination 1- hexyl -2-methylimidazole by weight:3, extract 3h under room temperature；
（5）Suction filtration is separated：4 times of absolute ethyl alcohol is added in passion fruit slurry after extraction, at room temperature suction filtration is separated, and in 34 DEG C
Lower rotary evaporation in vacuo；
（6）Freeze concentration：Slag after evaporation freezes 16h at being placed in -20 DEG C, preserves after crushing.
Embodiment 4
A kind of preparation method of passion fruit compound sugar, comprises the following steps：
（1）Choose fruit：The tan passion fruit of passion fruit skin is chosen, corrupt, rotten fruit is removed；
（2）Peeling, stripping seed：Staff peels off passion fruit appearance, retains middle yellow pulp；
（3）Beating：By passion fruit pulp and water by weight 1:18 mixing, are placed in beater and are beaten 50s；
（4）Super-ionic liquid Microwave Extraction compound sugar：Using bromination 1- hexyl -2-methylimidazole as extraction solution, passion fruit meat
Slurry is 1 with bromination 1- hexyl -2-methylimidazole by weight:4, extract 4h under room temperature；
（5）Suction filtration is separated：4 times of absolute ethyl alcohol is added in passion fruit slurry after extraction, at room temperature suction filtration is separated, and in 38 DEG C
Lower rotary evaporation in vacuo；
（6）Freeze concentration：Slag after evaporation freezes 22h at being placed in -15 DEG C, preserves after crushing.
Embodiment 5
A kind of preparation method of passion fruit compound sugar, comprises the following steps：
（1）Choose fruit：The tan passion fruit of passion fruit skin is chosen, corrupt, rotten fruit is removed；
（2）Peeling, stripping seed：Staff peels off passion fruit appearance, retains middle yellow pulp；
（3）Beating：By passion fruit pulp and water by weight 1:20 mixing, are placed in beater and are beaten 60s；
（4）Super-ionic liquid Microwave Extraction compound sugar：Using bromination 1- hexyl -2-methylimidazole as extraction solution, passion fruit meat
Slurry is 1 with bromination 1- hexyl -2-methylimidazole by weight:5, extract 5h under room temperature；
（5）Suction filtration is separated：5 times of absolute ethyl alcohol is added in passion fruit slurry after extraction, at room temperature suction filtration is separated, and in 40 DEG C
Lower rotary evaporation in vacuo；
（6）Freeze concentration：Slag after evaporation freezes 24h at being placed in -10 DEG C, preserves after crushing.
Claims (3)
1. a kind of preparation method of passion fruit compound sugar, it is characterised in that：Comprise the steps：
（1）Choose fruit：The tan passion fruit of passion fruit skin is chosen, corrupt, rotten fruit is removed；
（2）Peeling, stripping seed：Staff peels off passion fruit appearance, retains middle yellow pulp；
（3）Beating：By passion fruit pulp and water by weight 1:10-1:20 mixing, are placed in beater and are beaten 20-60s；
（4）Super-ionic liquid Microwave Extraction compound sugar：Using bromination 1- hexyl -2-methylimidazole as extraction solution, passion fruit meat
Slurry is 1 with bromination 1- hexyl -2-methylimidazole by weight:1-1:5, extract 1-5h under room temperature；
（5）Suction filtration is separated：2-5 times of absolute ethyl alcohol is added in passion fruit slurry after extraction, at room temperature suction filtration is separated, and in
Rotary evaporation in vacuo at 30-40 DEG C；
（6）Freeze concentration：Slag after evaporation freezes 12-24h at being placed in -30 DEG C to -10 DEG C, preserves after crushing.
2. the preparation method of a kind of passion fruit compound sugar according to claim 1, it is characterised in that：Described super-ionic liquid
Body bromination 1- hexyl -2-methylimidazole also can be substituted with 1- butyl -3- methyl imidazolium tetrafluoroborates.
3. the preparation method of a kind of passion fruit compound sugar according to claim 1, it is characterised in that：Described preparation method
It is applicable not only to extract passion fruit compound sugar, is also applicable to extract apple, the compound sugar of strawberry.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611226999.7A CN106632528A (en) | 2016-12-27 | 2016-12-27 | Preparation method of passion fruit oligosaccharide |
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| CN201611226999.7A CN106632528A (en) | 2016-12-27 | 2016-12-27 | Preparation method of passion fruit oligosaccharide |
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| CN201611226999.7A Pending CN106632528A (en) | 2016-12-27 | 2016-12-27 | Preparation method of passion fruit oligosaccharide |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108610432A (en) * | 2018-04-03 | 2018-10-02 | 徐州工程学院 | A kind of method of high efficiency extraction edible burdock inulin |
Citations (7)
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|---|---|---|---|---|
| CN1070195A (en) * | 1992-09-04 | 1993-03-24 | 中国人民武装警察部队河北省总队医院 | A kind of method of from soyflour, extracting oligose |
| CN1099393A (en) * | 1993-08-26 | 1995-03-01 | 石家庄市美亚实业总公司 | Method for preparation of oligosaccharilde liquid from dregs of bean |
| CN1259519A (en) * | 1998-12-12 | 2000-07-12 | 中国科学院新疆化学研究所 | Purification method of oligosaccharide |
| CN101395184A (en) * | 2006-03-08 | 2009-03-25 | 巴斯夫欧洲公司 | Method for breaking down cellulose in solution |
| CN103467615A (en) * | 2013-09-17 | 2013-12-25 | 南京通泽农业科技有限公司 | Preparation method of lycium barbarum oligosaccharide |
| CN103467614A (en) * | 2013-09-17 | 2013-12-25 | 南京通泽农业科技有限公司 | Process for extracting yam oligosaccharide |
| CN104448017A (en) * | 2014-11-27 | 2015-03-25 | 秦富英 | Method for extracting Chinese yam oligosaccharide |
-
2016
- 2016-12-27 CN CN201611226999.7A patent/CN106632528A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1070195A (en) * | 1992-09-04 | 1993-03-24 | 中国人民武装警察部队河北省总队医院 | A kind of method of from soyflour, extracting oligose |
| CN1099393A (en) * | 1993-08-26 | 1995-03-01 | 石家庄市美亚实业总公司 | Method for preparation of oligosaccharilde liquid from dregs of bean |
| CN1259519A (en) * | 1998-12-12 | 2000-07-12 | 中国科学院新疆化学研究所 | Purification method of oligosaccharide |
| CN101395184A (en) * | 2006-03-08 | 2009-03-25 | 巴斯夫欧洲公司 | Method for breaking down cellulose in solution |
| CN103467615A (en) * | 2013-09-17 | 2013-12-25 | 南京通泽农业科技有限公司 | Preparation method of lycium barbarum oligosaccharide |
| CN103467614A (en) * | 2013-09-17 | 2013-12-25 | 南京通泽农业科技有限公司 | Process for extracting yam oligosaccharide |
| CN104448017A (en) * | 2014-11-27 | 2015-03-25 | 秦富英 | Method for extracting Chinese yam oligosaccharide |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108610432A (en) * | 2018-04-03 | 2018-10-02 | 徐州工程学院 | A kind of method of high efficiency extraction edible burdock inulin |
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Application publication date: 20170510 |