CN104448017A - Method for extracting Chinese yam oligosaccharide - Google Patents
Method for extracting Chinese yam oligosaccharide Download PDFInfo
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- CN104448017A CN104448017A CN201410699698.0A CN201410699698A CN104448017A CN 104448017 A CN104448017 A CN 104448017A CN 201410699698 A CN201410699698 A CN 201410699698A CN 104448017 A CN104448017 A CN 104448017A
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Abstract
The invention provides a method for extracting Chinese yam oligosaccharide. The method comprises the following steps: selecting fresh Chinese yam; washing and slicing; drying for 9 to 10 hours at 60 DEG C; crushing; sieving by a 180-micron sieve; performing ultrasonic auxiliary extracting; leaching and concentrating; deproteinizing by a sevage method; performing alcohol precipitation; taking supernatant; concentrating; freeze-drying to obtain an oligosaccharide extract. The method for auxiliary extraction of the Chinese yam oligosaccharide by utilizing ultrasonic waves is low in cost and short in time consumption; optimal extracting conditions for extracting the oligosaccharide are adopted, so that the extracting efficiency is high; the alcohol precipitation is performed after the deproteinizing is performed by adopting the sevage method, so that the purity of the oligosaccharide is high. The extracting method provided by the invention is simple and is easy to operate, and is applicable to industrial production.
Description
Technical field
The present invention relates to a kind of method extracting oligose, be specifically related to a kind of method extracting yam oligosaccharide.
Background technology
Oligose is also known as making oligosaccharides, be by the polymkeric substance of 2 ~ 10 monose through glycosidic link dehydrating condensation, have and improve micro-ecological environment in human body, improve blood lipid metabolism, strengthen immunologic function, delay senility, promote the physiological functions such as the absorption of the mineral substance such as calcium and iron, anticancer, preventing dental caries.At present, the research for functional oligose attracts wide attention.Oligose is widely used in, in milk-product, lactobacillus drink, bifidobacterium yoghurt, cereal foods and protective foods, being especially applied in the food of infant and the elderly as a kind of food ingredients.In health food series, also having is separately the oral liquid that raw material is made with oligose, be directly used for regulating intestinal canal flora, relax bowel, adjusting blood lipid, immunity moderation etc.Chinese yam is that Dioscoreaceae is overgrow the rhizome of herbaceous plant Chinese yam for many years, and having another name called Rhizoma dioscoreae, hundred medicines, fan potato etc., is a kind of traditional integration of drinking and medicinal herbs food of China.In recent years, comparatively deep research has been carried out to the functional health compositions such as Chinese yam polysaccharide, glycoprotein, choline and application thereof both at home and abroad, and about the extraction and separation technology of yam oligosaccharide significant for instructing it to develop further.
The method of current extraction yam oligosaccharide, mainly contains immersion method and microwave method, but these methods exist following defect: immersion method, and length consuming time, extraction yield are low; Microwave method, cost is high, extraction yield is not high yet.But in the extraction process of Chinese yam activeconstituents, ultrasonic technique has been applied to the materials such as Chinese yam polysaccharide, dioscin be bright, but have no report temporarily about the research of ultrasonic-assisted extraction yam oligosaccharide.
Summary of the invention
For the deficiencies in the prior art, the object of this invention is to provide a kind of consuming time short, cost is low, the method for high extraction, highly purified extraction yam oligosaccharide.
In order to achieve the above object, the technical scheme that the present invention takes is:
Extract a method for yam oligosaccharide, comprise the following steps:
(1) choose fresh Chinese yam, section after cleaning, the drying baker being placed in 60 DEG C dries 9 ~ 10h, then it is for subsequent use to cross 180um sieve after being ground into powder;
(2) accurately taking Chinese yam powder, is that 1:13 ~ 1:18 adds the agent of concentration 55% ~ 65% extraction using alcohol by solid-liquid ratio, is placed in Ultrasonic Cleaners extraction 50 ~ 70min that power is 500W;
(3) after to be extracted, suction filtration concentrates, and adds the ethanol that concentration is 60%, get supernatant liquor and concentrate, can obtain oligose after lyophilize after impurity all precipitates after adopting sevage method deproteinated.
As optimal technical scheme, in order to reach the optimal conditions extracting yam oligosaccharide, be beneficial to improve extraction yield and purity, preferably extraction time is 60min to be placed in ultrasonic cleaner in described step (2).
As optimal technical scheme, in order to reach the optimal conditions extracting yam oligosaccharide, be beneficial to improve extraction yield and purity, the preferred concentration adding extraction agent ethanol in described step (2) is 60%.
As optimal technical scheme, in order to reach the optimal conditions extracting yam oligosaccharide, be beneficial to improve extraction yield and purity, the middle Chinese yam powder of described step (2) and extraction agent ethanol preferably solid-liquid ratio are 1:15.
Compared with prior art, the beneficial effect that the present invention has: the method utilizing ultrasonic-assisted extraction yam oligosaccharide, cost is low, consuming time short; Take to extract the optimum extraction conditions of oligose, extraction yield is high; Adopt the pure rear alcohol precipitation of sevage method deproteinated, oligose purity is high.Extracting method of the present invention is simple, easy handling, is applicable to suitability for industrialized production.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described.
Embodiment 1
Extract a method for yam oligosaccharide, comprise the following steps:
(1) choose fresh Chinese yam, section after cleaning, the drying baker being placed in 60 DEG C dries 9h, then it is for subsequent use to cross 180um sieve after being ground into powder;
(2) accurately taking Chinese yam powder, is that 1:13 adds the agent of concentration 55% extraction using alcohol by solid-liquid ratio, and being placed in power is that the Ultrasonic Cleaners of 500W extracts 50min;
(3) after to be extracted, suction filtration concentrates, and adds the ethanol that concentration is 60%, get supernatant liquor and concentrate, can obtain oligose after lyophilize after impurity all precipitates after adopting sevage method deproteinated.
Embodiment 2
Extract a method for yam oligosaccharide, comprise the following steps:
(1) choose fresh Chinese yam, section after cleaning, the drying baker being placed in 60 DEG C dries 10h, then it is for subsequent use to cross 180um sieve after being ground into powder;
(2) accurately taking Chinese yam powder, is that 1:15 adds the agent of concentration 60% extraction using alcohol by solid-liquid ratio, and being placed in power is that the Ultrasonic Cleaners of 500W extracts 60min;
(3) after to be extracted, suction filtration concentrates, and adds the ethanol that concentration is 60%, get supernatant liquor and concentrate, can obtain oligose after lyophilize after impurity all precipitates after adopting sevage method deproteinated.
Embodiment 3
Extract a method for yam oligosaccharide, comprise the following steps:
(1) choose fresh Chinese yam, section after cleaning, the drying baker being placed in 60 DEG C dries 9h, then it is for subsequent use to cross 180um sieve after being ground into powder;
(2) accurately taking Chinese yam powder, is that 1:18 adds the agent of concentration 65% extraction using alcohol by solid-liquid ratio, and being placed in power is that the Ultrasonic Cleaners of 500W extracts 70min;
(3) after to be extracted, suction filtration concentrates, and adds the ethanol that concentration is 60%, get supernatant liquor and concentrate, can obtain oligose after lyophilize after impurity all precipitates after adopting sevage method deproteinated.
It should be pointed out that above case is only the representational example of the present invention.Obviously, technical scheme of the present invention is not limited to above-described embodiment, can also have many sex change.
Claims (4)
1. extract a method for yam oligosaccharide, it is characterized in that, comprise the following steps:
(1) choose fresh Chinese yam, section after cleaning, the drying baker being placed in 60 DEG C dries 9 ~ 10h, then it is for subsequent use to cross 180um sieve after being ground into powder;
(2) accurately taking Chinese yam powder, is that 1:13 ~ 1:18 adds the agent of concentration 55% ~ 65% extraction using alcohol by solid-liquid ratio, is placed in Ultrasonic Cleaners extraction 50 ~ 70min that power is 500W;
(3) after to be extracted, suction filtration concentrates, and adds the ethanol that concentration is 60%, get supernatant liquor and concentrate, can obtain oligose after lyophilize after impurity all precipitates after adopting sevage method deproteinated.
2. a kind of method extracting yam oligosaccharide according to claim 1, is characterized in that: preferably extraction time is 60min to be placed in ultrasonic cleaner in described step (2).
3. a kind of method extracting yam oligosaccharide according to claim 1 ~ 2 any one, is characterized in that: the preferred concentration adding extraction agent ethanol in described step (2) is 60%.
4. a kind of method extracting yam oligosaccharide according to claims 1 to 3 any one, is characterized in that: the middle Chinese yam powder of described step (2) and extraction agent ethanol preferably solid-liquid ratio are 1:15.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410699698.0A CN104448017A (en) | 2014-11-27 | 2014-11-27 | Method for extracting Chinese yam oligosaccharide |
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| CN201410699698.0A CN104448017A (en) | 2014-11-27 | 2014-11-27 | Method for extracting Chinese yam oligosaccharide |
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| CN104448017A true CN104448017A (en) | 2015-03-25 |
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| CN201410699698.0A Pending CN104448017A (en) | 2014-11-27 | 2014-11-27 | Method for extracting Chinese yam oligosaccharide |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106343306A (en) * | 2016-08-24 | 2017-01-25 | 韦克康 | Method for preparing healthy oral liquid |
| CN106632528A (en) * | 2016-12-27 | 2017-05-10 | 温县兴发生物科技有限公司 | Preparation method of passion fruit oligosaccharide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1861637A (en) * | 2006-06-20 | 2006-11-15 | 江苏省中国科学院植物研究所 | Preparation process of Huai Chinese yam polysaccharose and use thereof |
| CN102206291A (en) * | 2011-04-11 | 2011-10-05 | 北京林业大学 | Process for extracting yam polysaccharide by using supercritical CO2 fluid extraction technique |
| CN103467614A (en) * | 2013-09-17 | 2013-12-25 | 南京通泽农业科技有限公司 | Process for extracting yam oligosaccharide |
-
2014
- 2014-11-27 CN CN201410699698.0A patent/CN104448017A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1861637A (en) * | 2006-06-20 | 2006-11-15 | 江苏省中国科学院植物研究所 | Preparation process of Huai Chinese yam polysaccharose and use thereof |
| CN102206291A (en) * | 2011-04-11 | 2011-10-05 | 北京林业大学 | Process for extracting yam polysaccharide by using supercritical CO2 fluid extraction technique |
| CN103467614A (en) * | 2013-09-17 | 2013-12-25 | 南京通泽农业科技有限公司 | Process for extracting yam oligosaccharide |
Non-Patent Citations (1)
| Title |
|---|
| 刘露等: ""响应面法优化超声波辅助提取山药低聚糖的工艺研究"", 《广东农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106343306A (en) * | 2016-08-24 | 2017-01-25 | 韦克康 | Method for preparing healthy oral liquid |
| CN106632528A (en) * | 2016-12-27 | 2017-05-10 | 温县兴发生物科技有限公司 | Preparation method of passion fruit oligosaccharide |
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Application publication date: 20150325 |