CN101514234A - Blackfungus polyhexose extraction method - Google Patents
Blackfungus polyhexose extraction method Download PDFInfo
- Publication number
- CN101514234A CN101514234A CNA2009100715056A CN200910071505A CN101514234A CN 101514234 A CN101514234 A CN 101514234A CN A2009100715056 A CNA2009100715056 A CN A2009100715056A CN 200910071505 A CN200910071505 A CN 200910071505A CN 101514234 A CN101514234 A CN 101514234A
- Authority
- CN
- China
- Prior art keywords
- extraction
- blackfungus polyhexose
- vacuum
- auricularia polycose
- blackfungus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
A blackfungus polyhexose extraction method relates to a Blackfungus polyhexose preparation method. The invention solves the problems of low extraction rate, low activity and fussy production technique existing in extracting the blackfungus polyhexose with the existing traditional method. The technical steps of the blackfungus polyhexose extraction method are as follows: blackfungus is selected, sliced and cracked, and is extracted by a strong electric field; then a supercentrifuge is used for separating the extraction liquid and removing the protein and lipid materials, and then a vacuum scraper type film is used for concentrating, and a vacuum freezedryer is used for freeze-drying, thus obtaining the freeze-dried powder of the blackfungus polyhexose. The blackfungus polyhexose prepared by the invention is a natural extract, does not have side effects on the human bodies, and has the functions like increasing the immunologic function, resisting knubs, reducing blood-fat and blood pressure, resisting cruor and thrombus, losing weight, caring beauty and skin, and the like. The blackfungus polyhexose can be broadly applied in the fields like food, health products, cosmetics, medicines, biochemical industry, and the like.
Description
Technical field
The present invention relates to a kind of blackfungus polyhexose preparation method.
Background technology
Auricularia polycose is the sp act factor of black fungus, has effects such as raise immunity, antitumor, reducing blood-fat, hypotensive, anticoagulation, antithrombotic, fat-reducing, beauty and skin care.Can be applicable to fields such as food, healthcare products, makeup, medicine, biochemical industry.
Auricularia polycose can be widely used in food, medicine, healthcare products, cosmetics production at present, is the worldwide multifunctional product of fashionable America and Europe, Japan and Korea S., and its market outlook are boundless.Though it is at present a lot of to the research of Auricularia polycose, but the extracting method of Auricularia polycose is mainly based on supersonic method and reflux extraction method, with producing a large amount of free radicals in the supersonic method extraction Auricularia polycose process, destroy the Auricularia polycose physiologically active, and the reflux extraction method is a kind of high temperature abstraction technique, can make Auricularia polycose lose physiological function equally, and the extraction yield of two kinds of methods is relatively low.This invention proposes highfield extraction Auricularia polycose, has well kept activity, has very high nutritive value, can be used as the raw material of food, medicine, healthcare products, makeup.
Summary of the invention
Low in order to solve the Auricularia polycose extraction yield, active not high problem the invention provides the method that a kind of highfield legal system is equipped with Auricularia polycose, can effectively improve the extraction yield and the product activity of Auricularia polycose.Among the present invention, a kind of Blackfungus polyhexose extraction method, the step that it is characterized in that the method that Auricularia polycose extracts is as follows: one, the extraction of Auricularia polycose: carry out the screening of black fungus earlier, choose the black fungus of plucking fresh autumn, dry, pulverize, the ratio that with the solid-liquid ratio is 1g: 25mL～1g: 40mL adds the black fungus powder and the aqueous solution respectively, with highfield extraction 40～60 minutes, obtain containing the Auricularia polycose extraction liquid, carry out solid-liquid separation with the horizontal helical type whizzer with the speed of 1500～2000r/min at last, isolate extracting solution and residue, with residue is the ratio adding aqueous solution of 1g: 25mL～1g: 40mL again with the solid-liquid ratio, proceeding highfield extracts and centrifugation, extract 3 times continuously, remove residue, united extraction liquid.Two, concentrate: the Auricularia polycose extracting solution is adopted vacuum scraper-type thin film concentration, and concentrated condition is 45～55 ℃ of temperature, and vacuum tightness is 0.1～0.15mbar, obtains black fungus Crude polysaccharides concentrated solution; Three, purifying: it is 20cm that black fungus Crude polysaccharides concentrated solution is added diameter by fluid distributor, it highly is the high efficiency chromatography chromatography column of 250cm, sodium chloride solution wash-out with concentration, flow velocity is 100mL～250mL/h, get the Auricularia polycose elutriant, concentrate with vacuum scraper-type thin film concentration equipment again.Four, drying: being ground into 60 order pressed powders after with Auricularia polycose concentrated solution vacuum-freeze-dry under 0.4～0.7mbar, temperature-40～-50 ℃ condition, promptly get the Auricularia polycose lyophilized powder with the vacuum-freeze-dry machine.
Among the present invention, in Blackfungus polyhexose extraction method, adopt the highfield auxiliary extraction to cooperate the method for the centrifugal cycling extraction of horizontal helical type, make Auricularia polycose be able to abundant release, make the extraction yield of polysaccharide can reach 85～92%, than the extraction yield of ultrasonic extraction or solvent refluxing extraction method high respectively 10～20% and 15～20%.Its specific activity extraction method ultrasonic extraction or solvent refluxing extraction method height 20～30%, improved the added economic value of Auricularia polycose greatly.Technology of the present invention is simple, extraction yield height, suitable promotion and application.The Auricularia polycose of the present invention's preparation is active high, can be used as the raw material that can be used as food, medicine, healthcare products, makeup.
Embodiment
Embodiment one: in this embodiment, a kind of Blackfungus polyhexose extraction method, the step that it is characterized in that the method that Auricularia polycose extracts is as follows: one, the extraction of Auricularia polycose: carry out the screening of black fungus earlier, choose the black fungus of plucking fresh autumn, dry, pulverize, the ratio that with the solid-liquid ratio is 1g: 25mL～1g: 40mL adds the black fungus powder and the aqueous solution respectively, with highfield extraction 40～60 minutes, obtain containing the Auricularia polycose extraction liquid, carry out solid-liquid separation with the horizontal helical type whizzer with the speed of 1500～2000r/min at last, isolate extracting solution and residue, with residue is the ratio adding aqueous solution of 1g: 25mL～1g: 40mL again with the solid-liquid ratio, proceeding highfield extracts and centrifugation, extract 3 times continuously, remove residue, united extraction liquid.Two, concentrate: the Auricularia polycose extracting solution is adopted vacuum scraper-type thin film concentration, and concentrated condition is 45～55 ℃ of temperature, and vacuum tightness is 0.1～0.15mbar, obtains black fungus Crude polysaccharides concentrated solution; Three, purifying: it is 20cm that black fungus Crude polysaccharides concentrated solution is added diameter by fluid distributor, it highly is the high efficiency chromatography chromatography column of 250cm, sodium chloride solution wash-out with concentration, flow velocity is 100mL～250mL/h, get the Auricularia polycose elutriant, concentrate with vacuum scraper-type thin film concentration equipment again.Four, drying: being ground into 60 order pressed powders after with Auricularia polycose concentrated solution vacuum-freeze-dry under 0.4～0.7mbar, temperature-40～-50 ℃ condition, promptly get the Auricularia polycose lyophilized powder with the vacuum-freeze-dry machine.
Embodiment two: what this embodiment and embodiment one were different is: extraction strength of electric field is 60～90 kilovolts every square centimeter in the step 1, high-voltage electric field strokes per minute 600～800 times, pulse-repetition 300～500Hz.Other is identical with embodiment one.
Embodiment three: what this embodiment and embodiment one were different is: it is characterized in that in the step 2 that solid content is 50%～70% in the concentrated solution.Other is identical with embodiment one.
Embodiment four: what this embodiment and embodiment one were different is: it is characterized in that high efficiency chromatography chromatography column purifying in the step 3, used solid-phase media is the X-5 macroporous resin, and the moving phase medium is for being 0.5%～1.5% sodium-chlor wash-out with mass concentration.Other is identical with embodiment one.
Embodiment five: what this embodiment and embodiment one were different is: the vacuum-freeze-dry time is 24～48 hours in the step 3.
The extraction yield of Auricularia polycose is 85～92% in this embodiment, and purity is 70～80%, and the gained Auricularia polycose is active high, quality better.
Claims (4)
- A kind of Blackfungus polyhexose extraction method, the step that it is characterized in that the method that Auricularia polycose extracts is as follows: one, the extraction of Auricularia polycose: carry out the screening of black fungus earlier, choose the black fungus of plucking fresh autumn, dry, pulverize, the ratio that with the solid-liquid ratio is 1g: 25mL～1g: 40mL adds the black fungus powder and the aqueous solution respectively, with highfield extraction 40～60 minutes, obtain containing the Auricularia polycose extraction liquid, carry out solid-liquid separation with the horizontal helical type whizzer with the speed of 1500～2000r/min at last, isolate extracting solution and residue, with residue is the ratio adding aqueous solution of 1g: 25mL～1g: 40mL again with the solid-liquid ratio, proceeding highfield extracts and centrifugation, extract 3 times continuously, remove residue, united extraction liquid.Two, concentrate: the Auricularia polycose extracting solution is adopted vacuum scraper-type thin film concentration, and concentrated condition is 45～55 ℃ of temperature, and vacuum tightness is 0.1～0.15mbar, obtains black fungus Crude polysaccharides concentrated solution; Three, purifying: it is 20cm that black fungus Crude polysaccharides concentrated solution is added diameter by fluid distributor, highly be the high efficiency chromatography chromatography column of 250cm, use the sodium chloride solution wash-out, flow velocity is 100mL～250mL/h, get the Auricularia polycose elutriant, concentrate with vacuum scraper-type thin film concentration equipment again.Four, drying: being ground into 60 order pressed powders after with Auricularia polycose concentrated solution vacuum-freeze-dry under 0.4～0.7mbar, temperature-40～-50 ℃ condition, promptly get the Auricularia polycose lyophilized powder with the vacuum-freeze-dry machine.1, a kind of Blackfungus polyhexose extraction method according to claim 1 is characterized in that extraction strength of electric field is 60～90 kilovolts every square centimeter in the step 1, high-voltage electric field strokes per minute 600～800 times, pulse-repetition 300～500Hz.
- 2, a kind of Blackfungus polyhexose extraction method according to claim 1 is characterized in that in the step 2 that solid content is 50%～70% in the concentrated solution.
- 3, a kind of Blackfungus polyhexose extraction method according to claim 1 is characterized in that high efficiency chromatography chromatography column purifying in the step 3, and used solid-phase media is the X-5 macroporous resin, and the moving phase medium is for being 0.5%～1.5% sodium-chlor wash-out with mass concentration.
- 4, a kind of Blackfungus polyhexose extraction method according to claim 1 is characterized in that the vacuum-freeze-dry time is 24～48 hours in the step 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100715056A CN101514234A (en) | 2009-03-09 | 2009-03-09 | Blackfungus polyhexose extraction method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100715056A CN101514234A (en) | 2009-03-09 | 2009-03-09 | Blackfungus polyhexose extraction method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101514234A true CN101514234A (en) | 2009-08-26 |
Family
ID=41038864
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2009100715056A Pending CN101514234A (en) | 2009-03-09 | 2009-03-09 | Blackfungus polyhexose extraction method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101514234A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101798358A (en) * | 2010-03-02 | 2010-08-11 | 浙江工商大学 | Method for separating and preparing beta-glucan from black funguses |
| CN101812137A (en) * | 2009-12-28 | 2010-08-25 | 大兴安岭科丽尔生物工程有限责任公司 | Method for enriching agaric polysaccharide in purified black agaric by using macroporous absorption resin |
| CN103520451A (en) * | 2013-10-19 | 2014-01-22 | 杨晓虹 | Allium cepa L.var.agrogatum don. composition with blood lipid reduction effect |
| CN104055010A (en) * | 2013-03-19 | 2014-09-24 | 王振宇 | Black fungus polysaccharide composite weight-reducing congee powder and preparation method thereof |
| CN105998092A (en) * | 2016-07-05 | 2016-10-12 | 华宝食用香精香料（上海）有限公司 | Method for extracting polyphenol from Yunnan auricularia auricula |
| CN110467684A (en) * | 2018-05-11 | 2019-11-19 | 东北林业大学 | A kind of method of cation macroreticular resin purification Blackfungus polyhexose |
-
2009
- 2009-03-09 CN CNA2009100715056A patent/CN101514234A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812137A (en) * | 2009-12-28 | 2010-08-25 | 大兴安岭科丽尔生物工程有限责任公司 | Method for enriching agaric polysaccharide in purified black agaric by using macroporous absorption resin |
| CN101812137B (en) * | 2009-12-28 | 2014-02-19 | 大兴安岭科丽尔生物工程有限责任公司 | Method for enriching agaric polysaccharide in purified black agaric by using macroporous absorption resin |
| CN101798358A (en) * | 2010-03-02 | 2010-08-11 | 浙江工商大学 | Method for separating and preparing beta-glucan from black funguses |
| CN101798358B (en) * | 2010-03-02 | 2011-11-16 | 浙江工商大学 | Method for separating and preparing beta-glucan from black funguses |
| CN104055010A (en) * | 2013-03-19 | 2014-09-24 | 王振宇 | Black fungus polysaccharide composite weight-reducing congee powder and preparation method thereof |
| CN103520451A (en) * | 2013-10-19 | 2014-01-22 | 杨晓虹 | Allium cepa L.var.agrogatum don. composition with blood lipid reduction effect |
| CN105998092A (en) * | 2016-07-05 | 2016-10-12 | 华宝食用香精香料（上海）有限公司 | Method for extracting polyphenol from Yunnan auricularia auricula |
| CN105998092B (en) * | 2016-07-05 | 2019-08-06 | 华宝香精股份有限公司 | A method of polyphenol is extracted from Yunnan black fungus |
| CN110467684A (en) * | 2018-05-11 | 2019-11-19 | 东北林业大学 | A kind of method of cation macroreticular resin purification Blackfungus polyhexose |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101692906B (en) | Apple polyphenol extracting method | |
| CN105949163B (en) | The method for extraction and purification of anthocyanidin in a kind of Black Box Tracing pomace | |
| CN101514234A (en) | Blackfungus polyhexose extraction method | |
| CN100590109C (en) | Technique for extracting cynamorlum herba cistanches medicinal materials effective component | |
| CN103665059B (en) | A kind of natural crocin extraction separation method and the preparation of blood lipid-lowering medicine thereof | |
| CN109134676A (en) | Oligosaccharide and preparation method thereof, application | |
| CN110272932A (en) | A kind of preparation method of lucid ganoderma spore powder polysaccharide peptide | |
| CN102060830A (en) | Method for extracting anthocyanin from fruit dregs of Nitraria sibirica Pall | |
| CN104017098A (en) | Method for directly extracting lentinus-edodes active polysaccharide from waste lentinus-edodes sticks | |
| CN104072627B (en) | A kind of preparation method of Herba Typhonii gigantei polyoses extract | |
| CN101829166A (en) | Big fruit sea-buckthorn pomace flavonoid and preparation method thereof | |
| CN101837059A (en) | Method for preparing apple polyphenol | |
| CN107573438A (en) | A kind of method that polysaccharide is extracted in the pericarp from passion fruit | |
| CN103044441B (en) | A kind of method extracting bilobalide from Ginkgo Leaf | |
| CN107286264A (en) | The deep working method of Chinese date nutrient material separation | |
| CN101445564A (en) | Method for extracting cervus elaphus Linnaeus polysaccharide | |
| CN101550196A (en) | Method for extracting pine seed polysaccharides of Korean pine | |
| CN101550284A (en) | Method for extracting pigment of hazelnut shell | |
| CN101642466B (en) | Preparation method of composition containing pilose antler polysaccharides and having function of reducing blood lipids | |
| CN102477105A (en) | Method for combined preparation of polysaccharide and flavone by using pumpkin stems and leaves as raw material | |
| CN111808905B (en) | Method for extracting walnut oligopeptide from low-temperature squeezed hickory cake and application thereof | |
| CN101554473A (en) | Method for preparing compound with hypoglycemic function containing cornu cervi pantotrichum insulin-like active factors | |
| CN106046079A (en) | Method for rapidly separating and preparing peonidin-3-caffeoyl sophoroside-5-glucoside from purple sweet potatoes | |
| CN104448017A (en) | Method for extracting Chinese yam oligosaccharide | |
| CN102627702A (en) | Extraction method of squid hyaluronic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20090826 |