CN101445564A - Method for extracting cervus elaphus Linnaeus polysaccharide - Google Patents
Method for extracting cervus elaphus Linnaeus polysaccharide Download PDFInfo
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- CN101445564A CN101445564A CNA200810209816XA CN200810209816A CN101445564A CN 101445564 A CN101445564 A CN 101445564A CN A200810209816X A CNA200810209816X A CN A200810209816XA CN 200810209816 A CN200810209816 A CN 200810209816A CN 101445564 A CN101445564 A CN 101445564A
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- elaphus linnaeus
- cervus elaphus
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Abstract
A method for extracting cervus elaphus Linnaeus polysaccharide relates to a method for preparing cervus elaphus Linnaeus polysaccharide and solves the problems of low cervus elaphus Linnaeus polysaccharide extracting rate, low activity and complex production process, which exist in a conventional method. The method for extracting cervus elaphus Linnaeus comprises the following steps: selecting, slicing and crushing ervus elaphus Linnaeus, extracting the ervus elaphus Linnaeus by using a strong electric field, separating extract with a supercentrifuge, removing albumen and lipid substances, carrying out concentration by using a vacuum scraper type film, and carrying out freeze-drying by using a vacuum freeze-dryer so as to obtain cervus elaphus Linnaeus polysaccharide freeze-dried powder. The cervus elaphus Linnaeus polysaccharide prepared by the invention is a natural extract, brings no side effect to human body, has the functions of strengthening immunologic function, resisting tumor and inflammation, adjusting lipid, resisting cruor and thrombus, promoting healing of cornea trauma, improving eye dryness, and the like, and can be applicable to the fields such as food, health products, cosmetics, medicine, biochemical industry, etc.
Description
Technical field
The present invention relates to a kind of cervus elaphus linnaeus polyoses producing method.
Background technology
Pilose antler polysaccharides (PAPS) is the sp act factor of pilose antler, be the acidic protein glycan of a class and protein bound, have the healing of raise immunity, antitumor, anti-inflammatory, accent fat, anticoagulation, antithrombotic, promotion corneal wound and improve effects such as eye dryness.
The cervus elaphus linnaeus polysaccharide can be widely used in food, medicine, healthcare products, cosmetics production at present, is the worldwide multifunctional product of fashionable America and Europe, Japan and Korea S., and its market outlook are boundless.Though it is at present a lot of to the research of pilose antler polysaccharides, but the extracting method of pilose antler polysaccharides is mainly based on supersonic method and reflux extraction method, with producing a large amount of free radicals in the supersonic method extraction pilose antler polysaccharides process, destroy the pilose antler polysaccharides physiologically active, and the reflux extraction method is a kind of high temperature abstraction technique, can make pilose antler polysaccharides lose physiological function equally, and the extraction yield of two kinds of methods have only the bright sample pilose antler of 3.0～5.0 μ g/g.This invention proposes highfield extraction pilose antler polysaccharides, has well kept activity, has very high nutritive value, can be used as the raw material of food, medicine, healthcare products, makeup.
Summary of the invention
Low in order to solve the cervus elaphus linnaeus polysaccharide extract rate, active not high problem the invention provides the method that a kind of highfield legal system is equipped with pilose antler polysaccharides, can effectively improve the extraction yield and the product activity of cervus elaphus linnaeus polysaccharide.Among the present invention, a kind of step of cervus elaphus linnaeus extraction method of polysaccharides is as follows: one, the extraction of cervus elaphus linnaeus polysaccharide: carry out the screening of cervus elaphus linnaeus earlier, choose the fresh young pilose antler of red deer, get tip 2/3rds places, section, pulverize, the ratio that with the solid-liquid ratio is 1g:15mL～1g:20mL adds the Pilose Antler and the aqueous solution respectively, with highfield extraction 25～35 minutes, obtain containing cervus elaphus linnaeus polysaccharide extraction liquid, carry out solid-liquid separation with the horizontal helical type whizzer with the speed of 1500～2000r/min at last, isolate extracting solution and residue, with residue is the ratio adding aqueous solution of 1g:15mL～1g:20mL again with the solid-liquid ratio, proceeds highfield and extracts and centrifugation, extracts continuously 3 times, remove residue, united extraction liquid.Two, concentrate: the cervus elaphus linnaeus polysaccharide extraction liquid is adopted vacuum scraper-type thin film concentration, and concentrated condition is 60～75 ℃ of temperature, and vacuum tightness is 0.1～0.15mbar, obtains pilose antler Crude polysaccharides concentrated solution; Three, purifying: pilose antler Crude polysaccharides concentrated solution is added the high efficiency chromatography chromatography column by fluid distributor, and with the sodium chloride solution wash-out of concentration, flow velocity is 20mL～25mL/h, gets the pilose antler polysaccharides elutriant, uses vacuum scraper-type thin film concentration equipment dense again.Four, drying: being ground into 60 order pressed powders after with cervus elaphus linnaeus polysaccharide concentrated solution vacuum-freeze-dry under 0.4～0.7mbar, temperature-40～-50 ℃ condition, promptly get cervus elaphus linnaeus polysaccharide frozen dried powder end with the vacuum-freeze-dry machine.
Among the present invention, in the cervus elaphus linnaeus extraction method of polysaccharides, adopt the highfield auxiliary extraction to cooperate the method for the centrifugal cycling extraction of horizontal helical type, make the cervus elaphus linnaeus polysaccharide be able to abundant release, make the extraction yield of polysaccharide can reach 80～90%, than the extraction yield of ultrasonic extraction or solvent refluxing extraction method high respectively 20～25% and 25～30%.Its specific activity extraction method ultrasonic extraction or solvent refluxing extraction method height 23～30%, improved the added economic value of cervus elaphus linnaeus polysaccharide greatly.Technology of the present invention is simple, extraction yield height, suitable promotion and application.The cervus elaphus linnaeus active polysaccharide height of the present invention's preparation can be used as the raw material that can be used as food, medicine, healthcare products, makeup.
Embodiment
Embodiment one: in this embodiment, a kind of step of cervus elaphus linnaeus extraction method of polysaccharides is as follows: one, the extraction of cervus elaphus linnaeus polysaccharide: carry out the screening of cervus elaphus linnaeus earlier, choose the fresh young pilose antler of red deer, get tip 2/3rds places, section, pulverize, the ratio that with the solid-liquid ratio is 1g:15mL～1g:20mL adds the Pilose Antler and the aqueous solution respectively, with highfield extraction 25～35 minutes, obtain containing cervus elaphus linnaeus polysaccharide extraction liquid, carry out solid-liquid separation with the horizontal helical type whizzer with the speed of 1500～2000r/min at last, isolate extracting solution and residue, with residue is the ratio adding aqueous solution of 1g:15mL～1g:20mL again with the solid-liquid ratio, proceeds highfield and extracts and centrifugation, extracts continuously 3 times, remove residue, united extraction liquid.Two, concentrate: the cervus elaphus linnaeus polysaccharide extraction liquid is adopted vacuum scraper-type thin film concentration, and concentrated condition is 60～75 ℃ of temperature, and vacuum tightness is 0.1～0.15mbar, obtains pilose antler Crude polysaccharides concentrated solution; Three, purifying: pilose antler Crude polysaccharides concentrated solution is added the high efficiency chromatography chromatography column by fluid distributor, and with the sodium chloride solution wash-out of concentration, flow velocity is 20mL～25mL/h, gets the pilose antler polysaccharides elutriant, uses vacuum scraper-type thin film concentration equipment dense again.Four, drying: being ground into 60 order pressed powders after with cervus elaphus linnaeus polysaccharide concentrated solution vacuum-freeze-dry under 0.4～0.7mbar, temperature-40～-50 ℃ condition, promptly get cervus elaphus linnaeus polysaccharide frozen dried powder end with the vacuum-freeze-dry machine.
Embodiment two: what this embodiment and embodiment one were different is: extraction strength of electric field is 60～90 kilovolts every square centimeter in the step 1, high-voltage electric field strokes per minute 600～800 times, pulse-repetition 200～300Hz.Other is identical with embodiment one.
Embodiment three: what this embodiment and embodiment one were different is: it is characterized in that in the step 2 that solid content is 70%～80% in the concentrated solution.Other is identical with embodiment one.
Embodiment four: what this embodiment and embodiment one were different is: it is characterized in that high efficiency chromatography chromatography column purifying in the step 3, used solid-phase media is X-5 macroporous resin, and the moving phase medium is for being 0.5%～0.7% sodium-chlor wash-out with mass concentration.Other is identical with embodiment one.
Embodiment five: what this embodiment and embodiment one were different is: dense vacuum-freeze-dry and time are 24～48 hours in the step 3.
The extraction yield of cervus elaphus linnaeus polysaccharide is 80～90% in this embodiment, and purity is 70～80%, gained cervus elaphus linnaeus active polysaccharide height, quality better.
Claims (5)
1, a kind of cervus elaphus linnaeus extraction method of polysaccharides, the step that it is characterized in that the method that pilose antler polysaccharides extracts is as follows: one, the extraction of cervus elaphus linnaeus polysaccharide: carry out the screening of cervus elaphus linnaeus earlier, choose the fresh young pilose antler of red deer, get tip 2/3rds places, section, pulverize, the ratio that with the solid-liquid ratio is 1g:15mL～1g:20mL adds the Pilose Antler and the aqueous solution respectively, with highfield extraction 25～35 minutes, obtain containing cervus elaphus linnaeus polysaccharide extraction liquid, carry out solid-liquid separation with the horizontal helical type whizzer with the speed of 1500～2000r/min at last, isolate extracting solution and residue, with residue is the ratio adding aqueous solution of 1g:15mL～1g:20mL again with the solid-liquid ratio, proceeds highfield and extracts and centrifugation, extracts continuously 3 times, remove residue, united extraction liquid.Two, concentrate: the cervus elaphus linnaeus polysaccharide extraction liquid is adopted vacuum scraper-type thin film concentration, and concentrated condition is 60～75 ℃ of temperature, and vacuum tightness is 0.1～0.15mbar, obtains pilose antler Crude polysaccharides concentrated solution; Three, purifying: pilose antler Crude polysaccharides concentrated solution is added the high efficiency chromatography chromatography column by fluid distributor, and with the sodium chloride solution wash-out of concentration, flow velocity is 20mL～25mL/h, gets the pilose antler polysaccharides elutriant, uses vacuum scraper-type thin film concentration equipment dense again.Four, drying: being ground into 60 order pressed powders after with cervus elaphus linnaeus polysaccharide concentrated solution vacuum-freeze-dry under 0.4～0.7mbar, temperature-40～-50 ℃ condition, promptly get cervus elaphus linnaeus polysaccharide frozen dried powder end with the vacuum-freeze-dry machine.
2, a kind of cervus elaphus linnaeus extraction method of polysaccharides according to claim 1 is characterized in that extraction strength of electric field is 60～90 kilovolts every square centimeter in the step 1, high-voltage electric field strokes per minute 600～800 times, pulse-repetition 200～300Hz.
3, a kind of cervus elaphus linnaeus extraction method of polysaccharides according to claim 1 is characterized in that in the step 2 that solid content is 70%～80% in the concentrated solution.
4, a kind of cervus elaphus linnaeus extraction method of polysaccharides according to claim 1 is characterized in that high efficiency chromatography chromatography column purifying in the step 3, and used solid-phase media is X-5 macroporous resin, and the moving phase medium is for being 0.5%～0.7% sodium-chlor wash-out with mass concentration.
5, a kind of cervus elaphus linnaeus extraction method of polysaccharides according to claim 1 is characterized in that dense vacuum-freeze-dry and time are 24～48 hours in the step 4.
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| CNA200810209816XA CN101445564A (en) | 2008-12-29 | 2008-12-29 | Method for extracting cervus elaphus Linnaeus polysaccharide |
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| CNA200810209816XA CN101445564A (en) | 2008-12-29 | 2008-12-29 | Method for extracting cervus elaphus Linnaeus polysaccharide |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948439A (en) * | 2010-08-17 | 2011-01-19 | 刘平 | Extraction method and application to medicine of active alkaloid compounds in cervus nippon temminck |
| CN102302515A (en) * | 2010-03-19 | 2012-01-04 | 董玲 | Hairy antler freeze-dried excipient and production method thereof |
| CN105884921A (en) * | 2014-11-26 | 2016-08-24 | 卢卫红 | Membrane separation method of sea cucumber polysaccharide fragments acting on simulation space heavy ion radiation oxidation stress |
| CN109122816A (en) * | 2018-08-21 | 2019-01-04 | 江苏大学 | A kind of preparation method and application of the black angle orchid extract of corona treatment |
-
2008
- 2008-12-29 CN CNA200810209816XA patent/CN101445564A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302515A (en) * | 2010-03-19 | 2012-01-04 | 董玲 | Hairy antler freeze-dried excipient and production method thereof |
| CN101948439A (en) * | 2010-08-17 | 2011-01-19 | 刘平 | Extraction method and application to medicine of active alkaloid compounds in cervus nippon temminck |
| CN101948439B (en) * | 2010-08-17 | 2012-08-22 | 刘平 | Extraction method and application to medicine of active alkaloid compounds in cervus nippon temminck |
| CN105884921A (en) * | 2014-11-26 | 2016-08-24 | 卢卫红 | Membrane separation method of sea cucumber polysaccharide fragments acting on simulation space heavy ion radiation oxidation stress |
| CN109122816A (en) * | 2018-08-21 | 2019-01-04 | 江苏大学 | A kind of preparation method and application of the black angle orchid extract of corona treatment |
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Application publication date: 20090603 |