CN102060830A - Method for extracting anthocyanin from fruit dregs of Nitraria sibirica Pall - Google Patents
Method for extracting anthocyanin from fruit dregs of Nitraria sibirica Pall Download PDFInfo
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- CN102060830A CN102060830A CN 201010596684 CN201010596684A CN102060830A CN 102060830 A CN102060830 A CN 102060830A CN 201010596684 CN201010596684 CN 201010596684 CN 201010596684 A CN201010596684 A CN 201010596684A CN 102060830 A CN102060830 A CN 102060830A
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Abstract
The invention relates to a method for extracting anthocyanin from the fruit dregs of Nitraria sibirica Pall. The method comprises the following working procedures of enzymolysis, extraction, separation, purification, elution, concentration and drying. The method in the invention is simple and the extraction rate is up to 80%, thus the resource can be utilized comprehensively; the method is beneficial for the industrialized production; and the method can provide a novel natural plant raw material of medicines and health food used for preventing tumor, reducing blood sugar, protecting the liver and soothing the gallbladder.
Description
One, technical field
The present invention relates to a kind of extracting method of anthocyanidin, especially a kind of method of from the Fructus Nitraria schoberi slag, extracting anthocyanidin.
Two, background technology
White thorn is the machaka of zygophyllaceae (Zygophyllaceae) Nitraria (Nitraria L.), and Mongolian is called " craze for Japanese pop culture is lattice not ", be commonly called as the ground jujube, mulberry, western sand cherry etc.For the common wild saline and alkaline plant in NORTHWEST CHINA area, very high economy, ecology and pharmaceutical use are arranged.Contain abundant nutritional activities composition, especially amino acid such as abundant VITAMIN, amino acid, polyose in the fruit, very easily be absorbed by the body and utilize, on average contain 0.37% anthocyanidin in the pomace.
Anthocyanidin (Anthocyanins) is a kind of water colo(u)r, belongs to flavonoid compound, and many forms with glucosides exist, and also claim anthocyanogen.Anthocyanidin is as a kind of natural food colour, has safe, nontoxic, aboundresources, pharmacological action outstanding feature, at aspects such as food, cosmetic, medicine huge application potential arranged.Because anthocyanidin belongs to flavonoids, has very strong antioxygenation, can remove intravital free radical, reduce oxidasic activity, improve the katabolism of the high glyceryl ester lipoprotein of human body, and can suppress cholesterol absorption, reduce low density lipoprotein cholesterol content; And having multiple functions such as resistance is different, antitumor, antianaphylaxis, protection gastric mucosa, is the activeconstituents that a class has nourishing function.
At present; white thorn resource is not only being brought into play very important effect in western China in ecological protection; and in medicine, protective foods performance history, become important natural high-quality resource gradually; as utilize nitraria sibirica pall juice exploitation nitraria fruit wine, sting beverage in vain, utilize the products such as soft capsule of oil of Nitraria seeds exploitation., in the process of utilizing nitraria sibirica pall juice, seed, pomace is wherein discarded, do not obtain utilizing.
Three, summary of the invention
Technical problem to be solved by this invention provides a kind of method of extracting anthocyanidin from the Fructus Nitraria schoberi slag.The present invention intends adopting following technical proposal: a kind of method of extracting anthocyanidin from the Fructus Nitraria schoberi slag, it is characterized in that described extracting method comprises enzymolysis, extraction, separation, purifying, wash-out, concentrated, drying process, the processing condition of enzymolysis operation are for getting the Fructus Nitraria schoberi slag, carrying out enzymic hydrolysis handles, employed zymin is a cellulase, and the addition of cellulase is 0.15～0.2% of a Fructus Nitraria schoberi slag weight, enzymic hydrolysis time 8～14min, temperature is 20～40 ℃, and the pH value is 3～4.6 in the use sodium-acetate regulatory enzyme hydrolytic process; The processing condition of abstraction process add 30～40% ethanol ultrasonic extraction in the material system after enzymic hydrolysis, Fructus Nitraria schoberi slag and alcoholic acid mass volume ratio (g: be 1: 10～20 mL), extraction time 15～25min, temperature is 45～55 ℃, ultrasonic power 150～250w, and the pH value of utilizing the sodium-acetate maintenance system in this ultrasonic extraction process is at 3～4.6 sour environment; The processing condition of separation circuit are to extracting solution whizzer centrifugation; The processing condition of purification procedures are carried out purifying for the anthocyanidin in the liquid after selecting non-polar resin to centrifugation, and resin absorption amount 50～70mg/g, eluting rate are 70～90%; The processing condition of wash-out operation are with 40～60% ethanol elutions; The processing condition of enrichment process are collected extracting solution and elutriant for merging, and 40～60 ℃ of temperature, concentrating under reduced pressure reclaims ethanol and gets enriched material under pressure 60～88kPa condition; The processing condition of drying process promptly get product for enriched material is heated to 60～80 ℃ of drying 3～6h in thermostatic drying chamber.
The present invention is the continuity that utilizes white thorn comprehensive utilization; be with protection and to recover desert, desert, saline and alkaline ecotope be prerequisite; the white thorn that has region feature and resources advantage with comprehensive utilization is a purpose; research and development utilize blue or green plain technology of Root of Vetchleaf Sophora and product innovation pattern, for the white thorn of mass-producing exploitation resource provides technical scheme.
The advantage that the technique scheme that the present invention adopts is extracted anthocyanidin is: extracting method is simple, extraction yield is up to 80%, the waste gas resource can be fully utilized, help suitability for industrialized production, also can be provided as prophylaxis of tumours, lowering blood glucose, hepatic cholagogic medicine and protective foods a kind of novel natural plant raw material is provided.
Four, embodiment
Embodiment 1: get 1000g Fructus Nitraria schoberi slag, carry out enzymic hydrolysis and handle, comprise cellulase in the employed zymin, and the addition of cellulase is 0.15% of a Fructus Nitraria schoberi slag weight, enzymic hydrolysis time 8min, 20 ℃ of temperature, and the pH value in the use sodium-acetate regulatory enzyme hydrolytic process is 3; Add 30% ethanol ultrasonic extraction in the material system after enzymic hydrolysis, Fructus Nitraria schoberi slag and alcoholic acid mass volume ratio (g: mL) be 1: 10, extraction time 15min, 45 ℃ of temperature, ultrasonic power 150w, and be that to utilize pH value in the sodium-acetate maintenance system be 3 sour environment in this ultrasonic extraction process; To extracting solution whizzer centrifugation, and the anthocyanidin in the liquid after selecting non-polar resin to centrifugation carries out purifying, resin absorption amount 50mg/g, eluting rate is 80%, uses 40% ethanol elution again, merges to collect extracting solution and elutriant, 40 ℃ of temperature, under the pressure 60kPa condition, concentrating under reduced pressure reclaims ethanol and gets enriched material, enriched material is inserted the blue or green element of the Root of Vetchleaf Sophora that is heated to 60 ℃ of dry 3.1g95% of getting of dry 6h in the thermostatic drying chamber again.
Embodiment 2: get 1000g Fructus Nitraria schoberi slag, carry out enzymic hydrolysis and handle, employed zymin is a cellulase, and the addition of cellulase is 0.18% of a Fructus Nitraria schoberi slag weight, enzymic hydrolysis time 10min, 30 ℃ of temperature, and the pH value in the use sodium-acetate regulatory enzyme hydrolytic process is 4; Add 35% ethanol ultrasonic extraction in the material system after enzymic hydrolysis, Fructus Nitraria schoberi slag and alcoholic acid mass volume ratio (g: mL) be 1: 15, extraction time 20min, 50 ℃ of temperature, ultrasonic power 200w, and be that to utilize pH value in the sodium-acetate maintenance system be 4 sour environment in this ultrasonic extraction process; To extracting solution whizzer centrifugation, and the anthocyanidin in the liquid after selecting non-polar resin to centrifugation carries out purifying, resin absorption amount 60mg/g, eluting rate is 70%, uses 50% ethanol elution again, merges to collect extracting solution and elutriant, 50 ℃ of temperature, under the pressure 70kPa condition, concentrating under reduced pressure reclaims ethanol and gets enriched material, enriched material is inserted the blue or green element of the Root of Vetchleaf Sophora that is heated to 70 ℃ of dry 3.05g95% of getting of dry 5h in the thermostatic drying chamber again.
Embodiment 3: get 1000g Fructus Nitraria schoberi slag, carry out enzymic hydrolysis and handle, comprise cellulase in the employed zymin, and the addition of cellulase is 0.2% of a raw material weight, enzymic hydrolysis time 14min, 40 ℃ of temperature, and the pH value 4.6 in the use sodium-acetate regulatory enzyme hydrolytic process; Add 40% ethanol ultrasonic extraction in the material system after enzymic hydrolysis, Fructus Nitraria schoberi slag and ethanol mass volume ratio (g: mL) be 1: 20, extraction time 25min, 55 ℃ of temperature, ultrasonic power 250w, and be that to utilize pH value in the sodium-acetate maintenance system be 4.6 sour environment in this ultrasonic extraction process; To extracting solution whizzer centrifugation, and the anthocyanidin in the liquid after selecting non-polar resin to centrifugation carries out purifying, resin absorption amount 70mg/g, eluting rate is 90%, uses 60% ethanol elution again, merges to collect extracting solution and elutriant, 60 ℃ of temperature, under the pressure 88kPa condition, concentrating under reduced pressure reclaims ethanol and gets enriched material, enriched material is inserted the blue or green element of the Root of Vetchleaf Sophora that is heated to 80 ℃ of dry 3.2g95% of getting of dry 3h in the thermostatic drying chamber again.
Claims (1)
1. method of from the Fructus Nitraria schoberi slag, extracting anthocyanidin, it is characterized in that described extracting method comprises enzymolysis, extraction, separation, purifying, wash-out, concentrated, drying process, the processing condition of enzymolysis operation are for getting the Fructus Nitraria schoberi slag, carrying out enzymic hydrolysis handles, employed zymin is a cellulase, and the addition of cellulase is 0.15～0.2% of a Fructus Nitraria schoberi slag weight, enzymic hydrolysis time 8～14min, temperature is 20～40 ℃, and the pH value is 3～4.6 in the use sodium-acetate regulatory enzyme hydrolytic process; The processing condition of abstraction process add 30～40% ethanol ultrasonic extraction in the material system after enzymic hydrolysis, Fructus Nitraria schoberi slag and alcoholic acid mass volume ratio (g: be 1: 10～20 mL), extraction time 15～25min, temperature is 45～55 ℃, ultrasonic power 150～250w, and the pH value of utilizing the sodium-acetate maintenance system in this ultrasonic extraction process is at 3～4.6 sour environment; The processing condition of separation circuit are to extracting solution whizzer centrifugation; The processing condition of purification procedures are carried out purifying for the anthocyanidin in the liquid after selecting non-polar resin to centrifugation, and resin absorption amount 50～70mg/g, eluting rate are 70～90%; The processing condition of wash-out operation are with 40～60% ethanol elutions; The processing condition of enrichment process are collected extracting solution and elutriant for merging, and 40～60 ℃ of temperature, under pressure 60～88kPa condition, concentrating under reduced pressure reclaims ethanol and gets enriched material; The processing condition of drying process promptly get product for enriched material is heated to 60～80 ℃ of drying 3～6h in thermostatic drying chamber.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991641A (en) * | 2010-11-09 | 2011-03-30 | 中国科学院西北高原生物研究所 | Preparation method of antioxidation nitraria tangutorum bobr extractive |
| CN102924423A (en) * | 2012-10-29 | 2013-02-13 | 杨公明 | Method for extracting cyanidin from banana peel |
| CN104983915A (en) * | 2015-07-23 | 2015-10-21 | 中国科学院西北高原生物研究所 | Preparation method of natural lycium ruthenicum composite antioxidant |
| CN106008442A (en) * | 2016-06-17 | 2016-10-12 | 青海清华博众生物技术有限公司 | Method for extracting anthocyanin from nitraria tangutorum peel residues |
| CN106174188A (en) * | 2016-07-12 | 2016-12-07 | 中国科学院西北高原生物研究所 | A kind of nitraria sibirica pall juice lyophilized powder and preparation method thereof |
| CN107212381A (en) * | 2017-04-20 | 2017-09-29 | 中国科学院西北高原生物研究所 | A kind of preparation method of white bur ferment |
| CN108743655A (en) * | 2018-07-03 | 2018-11-06 | 中南民族大学 | A kind of Sophora viciifolia chromocor extract and its preparation method and application |
| CN111407793A (en) * | 2020-03-25 | 2020-07-14 | 青海民族大学 | Method for preparing PD-1/PD-L1 inhibitor from nitraria tangutorum bobr |
| CN113336813A (en) * | 2021-06-18 | 2021-09-03 | 东北农业大学 | Natural anthocyanin preparation method based on nitraria fruit extract |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101760497A (en) * | 2009-12-07 | 2010-06-30 | 耿兆翔 | Microorganisms or/and biological enzyme extracting method for plant anthocyanin |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101760497A (en) * | 2009-12-07 | 2010-06-30 | 耿兆翔 | Microorganisms or/and biological enzyme extracting method for plant anthocyanin |
Non-Patent Citations (3)
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| 《中国食品添加剂》 20061231 吴澎等 超声波辅助法提取白刺果籽中原花青素的结构研究 41-46 1 , 第2期 2 * |
| 《山东农业大学学报(自然科学版)》 20051231 李永强等 白刺果实色素的提取及理化性质研究 75-81 1 第36卷, 第1期 2 * |
| 《食品与发酵工业》 20051231 吴澎等 超声波辅助法提取白刺果籽中原花青素的工艺 158-160 1 第31卷, 第5期 2 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991641A (en) * | 2010-11-09 | 2011-03-30 | 中国科学院西北高原生物研究所 | Preparation method of antioxidation nitraria tangutorum bobr extractive |
| CN102924423A (en) * | 2012-10-29 | 2013-02-13 | 杨公明 | Method for extracting cyanidin from banana peel |
| CN102924423B (en) * | 2012-10-29 | 2014-12-17 | 杨公明 | Method for extracting cyanidin from banana peel |
| CN104983915A (en) * | 2015-07-23 | 2015-10-21 | 中国科学院西北高原生物研究所 | Preparation method of natural lycium ruthenicum composite antioxidant |
| CN104983915B (en) * | 2015-07-23 | 2019-06-25 | 中国科学院西北高原生物研究所 | A kind of preparation method of black fruit fructus lycii natural composite antioxidant |
| CN106008442A (en) * | 2016-06-17 | 2016-10-12 | 青海清华博众生物技术有限公司 | Method for extracting anthocyanin from nitraria tangutorum peel residues |
| CN106174188A (en) * | 2016-07-12 | 2016-12-07 | 中国科学院西北高原生物研究所 | A kind of nitraria sibirica pall juice lyophilized powder and preparation method thereof |
| CN107212381A (en) * | 2017-04-20 | 2017-09-29 | 中国科学院西北高原生物研究所 | A kind of preparation method of white bur ferment |
| CN108743655A (en) * | 2018-07-03 | 2018-11-06 | 中南民族大学 | A kind of Sophora viciifolia chromocor extract and its preparation method and application |
| CN108743655B (en) * | 2018-07-03 | 2021-08-13 | 中南民族大学 | Rosa davurica flavone extract and preparation method and application thereof |
| CN111407793A (en) * | 2020-03-25 | 2020-07-14 | 青海民族大学 | Method for preparing PD-1/PD-L1 inhibitor from nitraria tangutorum bobr |
| CN113336813A (en) * | 2021-06-18 | 2021-09-03 | 东北农业大学 | Natural anthocyanin preparation method based on nitraria fruit extract |
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| CN102060830B (en) | 2012-06-13 |
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