CN103467614A - Process for extracting yam oligosaccharide - Google Patents
Process for extracting yam oligosaccharide Download PDFInfo
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- CN103467614A CN103467614A CN 201310423407 CN201310423407A CN103467614A CN 103467614 A CN103467614 A CN 103467614A CN 201310423407 CN201310423407 CN 201310423407 CN 201310423407 A CN201310423407 A CN 201310423407A CN 103467614 A CN103467614 A CN 103467614A
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- chinese yam
- oligose
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Abstract
The invention relates to a process for extracting a yam oligosaccharide, and in particular relates to a method for preparing a yam oligosaccharide by adopting a membrane separation technology. The process comprises the specific steps: drying and crushing of raw materials, degreasing, ethanol extraction, reduced pressure concentration, membrane separation, concentration, drying and the like. By adopting the membrane separation technology, pollution, solvent residue and phase change are avoided in the separation process; and the separation method is simple and easy to control, and can be applied to industrialized production. The oligosaccharide obtained in the process does not contain an organic solvent, so the oligosaccharide can be applied in the fields of food processing and medicines. The process plays a positive role in increasing the industrial value of yam, promoting the deep processing additional value of the yam and researching functional components of the yam.
Description
Technical field
The present invention relates to the deep processing technology of a kind of agricultural-food Chinese yam, be specifically related to a kind of Chinese yam oligose extraction process, belong to the processing of agriculture product technical field.
Background technology
Chinese yam has another name called Chinese yam, RHIIZOMA DIOSCOREAE from Henan of China, prolongs chapter, jade prolongs etc., and the piece root of the herbaceous plant Chinese yam Dioscorea opposita Thunb. of overgrowing for Dioscoreaceae is perennial, be a kind of nutritious traditional medicinal and edible plant.Compendium of Material Medica record Chinese yam distinguishes the flavor of that sweet property is flat, kidney-nourishing gas, strengthening the spleen and stomach, antidiarrheal dysentery, the saliva that reduces phlegm, profit fur.Modern pharmacological research shows, that the Chinese yam water decoction has is anti-ageing, hypoglycemic, strengthen immunomodulatory, anti-mutation and the function effect such as antitumor.There is substantial connection in activeconstituents in these effects and Chinese yam, and in Chinese yam, carbohydrate content is abundant, except containing much starch, also contains a certain amount of active polysaccharide and oligose.
Oligose is called again oligosaccharides, is the polymkeric substance formed through the glycosidic link dehydrating condensation by 2-10 monose, has micro-ecological environment in the human body of improvement, promotes the absorption of the mineral substance such as calcium, iron, improves the physiological functions such as immunity of organisms, anticancer, preventing dental caries.At present, the research for functional oligose attracts wide attention.Oligose is widely used in, in milk-product, lactobacillus drink, bifidobacterium yoghurt, cereal foods and protective foods, especially being applied in infant and the elderly's food as a kind of food ingredients.In health food series, also have and take separately the oral liquid that oligose makes as raw material, directly be used for the regulating intestinal canal flora, relax bowel, regulate blood fat, regulate immunity etc.The exploitation that this shows oligose has good market outlook.Extract oligose from Chinese yam, can effectively increase the industrial value of Chinese yam, promote Chinese yam deep processing added value.To the Chinese yam functional ingredient, research also plays active effect.
Summary of the invention
The present invention be directed to the demand of market to oligose, a kind of method of extracting oligose from Chinese yam is provided.The method adopts membrane separation process, and simple to operate, efficiency is high, can further be transformed into suitability for industrialized production.
The present invention is achieved by the following technical programs:
A kind of Chinese yam oligose extraction process, described technique comprises the following steps: dry Chinese yam → pulverizing → degreasing → alcohol steep → concentrating under reduced pressure → membrane sepn → collection filtrate → concentrating under reduced pressure → lyophilize → Chinese yam oligose.
Described technique specifically comprises the following steps:
(1) raw material is processed: after Chinese yam is pulverized, cross 60 mesh sieves.
(2) degreasing: selecting the sherwood oil of 60-90 ℃ of boiling range is degreasing reagent, and under 90 ℃ of conditions, refluxing extraction 3 hours, remove lipid component in Rhizoma Dioscoreae powder.
(3) alcohol steep: alcohol concn 40-80%, solid-liquid ratio is that 1:5-15 extracts temperature 30-80 ℃, extraction time 1-4h, extract 1-4 time repeatedly.
(4) concentrating under reduced pressure: alcohol extract is under 50 ℃ of conditions, and concentrating under reduced pressure is removed ethanol in vat liquor.
(5) membrane sepn: first cross the ultra-filtration membrane of 5000D after the concentrated solution dilution, see through the nanofiltration membrane of liquid after 500D, effective constituent is in trapped fluid.
(6) concentrating under reduced pressure: 500D film trapped fluid in step 5 is concentrated to the 60Brix left and right.
(7) lyophilize: by concentrated solution lyophilize in step 6, obtain the Chinese yam oligose.
Advantage of the present invention is: the oligose obtained in this invention does not contain organic solvent, can be for food processing field and field of medicaments; Adopt membrane separation technique, pollution-free in sepn process, no solvent residue and phase transformation produce; This separation method is simple, easily controls, and can be applicable to suitability for industrialized production.
Further illustrate the present invention below in conjunction with embodiment, but the scope of protection of present invention is not limited to following embodiment.
embodiment:
Embodiment 1:
(1) raw material is processed: after Chinese yam is pulverized, cross 60 mesh sieves.
(2) degreasing: selecting the sherwood oil of 60-90 ℃ of boiling range is degreasing reagent, and under 90 ℃ of conditions, refluxing extraction 3 hours, remove lipid component in Rhizoma Dioscoreae powder.
(3) alcohol steep: alcohol concn 40%, solid-liquid ratio is 1:5, extracts temperature 60 C, extraction time 2h, extract 3 times repeatedly.
(4) concentrating under reduced pressure: alcohol extract is under 50 ℃ of conditions, and concentrating under reduced pressure is removed ethanol in vat liquor.
(5) membrane sepn: first cross the ultra-filtration membrane of 5000D after the concentrated solution dilution, see through the nanofiltration membrane of liquid after 500D, effective constituent is in trapped fluid.
(6) concentrating under reduced pressure: 500D film trapped fluid in step 5 is concentrated to the 60Brix left and right.
(7) lyophilize: by concentrated solution lyophilize in step 6, obtain the Chinese yam oligose.
(8) detect: the Chinese yam oligose extraction yield that adopts the method to prepare is 3.59%, and through this molecular weight of oligosaccharide of high effective liquid chromatography for measuring, at 390-3102, the phenolsulfuric acid method is measured its purity 91.6%.
Embodiment 2:
(1) raw material is processed: after Chinese yam is pulverized, cross 60 mesh sieves.
(2) degreasing: selecting the sherwood oil of 60-90 ℃ of boiling range is degreasing reagent, and under 90 ℃ of conditions, refluxing extraction 3 hours, remove lipid component in Rhizoma Dioscoreae powder.
(3) alcohol steep: 60 ℃ of alcohol concn, extract temperature 60 C, extraction time 2h, extract 3 times repeatedly.
(4) concentrating under reduced pressure: alcohol extract is under 50 ℃ of conditions, and concentrating under reduced pressure is removed ethanol in vat liquor.
(5) membrane sepn: first cross the ultra-filtration membrane of 5000D after the concentrated solution dilution, see through the nanofiltration membrane of liquid after 500D, effective constituent is in trapped fluid.
(6) concentrating under reduced pressure: 500D film trapped fluid in step 5 is concentrated to the 60Brix left and right.
(7) lyophilize: by concentrated solution lyophilize in step 6, obtain the Chinese yam oligose.
(8) detect: the Chinese yam oligose extraction yield that adopts the method to prepare is 3.50%, and through this molecular weight of oligosaccharide of high effective liquid chromatography for measuring, at 371-3012, the phenolsulfuric acid method is measured its purity 92.9%.
Embodiment 3:
(1) raw material is processed: after Chinese yam is pulverized, cross 60 mesh sieves.
(2) degreasing: selecting the sherwood oil of 60-90 ℃ of boiling range is degreasing reagent, and under 90 ℃ of conditions, refluxing extraction 3 hours, remove lipid component in Rhizoma Dioscoreae powder.
(3) alcohol steep: alcohol concn 80%, extract temperature 60 C, extraction time 2h, extract 3 times repeatedly.
(4) concentrating under reduced pressure: alcohol extract is under 50 ℃ of conditions, and concentrating under reduced pressure is removed ethanol in vat liquor.
(5) membrane sepn: first cross the ultra-filtration membrane of 5000D after the concentrated solution dilution, see through the nanofiltration membrane of liquid after 500D, effective constituent is in trapped fluid.
(6) concentrating under reduced pressure: 500D film trapped fluid in step 5 is concentrated to the 60Brix left and right.
(7) lyophilize: by concentrated solution lyophilize in step 6, obtain the Chinese yam oligose.
(8) detect: the Chinese yam oligose extraction yield that adopts the method to prepare is 2.89%, and through this molecular weight of oligosaccharide of high effective liquid chromatography for measuring, at 349-3052, the phenolsulfuric acid method is measured its purity 93.7%.
Claims (5)
1. the extraction process of a Chinese yam oligose, is characterized in that concrete steps are as follows: dry Chinese yam → pulverizing → degreasing → alcohol steep → concentrating under reduced pressure → membrane sepn → collection filtrate → concentrating under reduced pressure → lyophilize → Chinese yam oligose.
2. the extraction process of a kind of Chinese yam oligose as claimed in claim 1, is characterized in that described defatting step: adopt sherwood oil (60-90 ℃) under 90 ℃ of conditions, refluxing extraction 1-5 hour, lipid component in the removal Rhizoma Dioscoreae powder.
3. the extraction process of a kind of Chinese yam oligose as claimed in claim 1, it is characterized in that described alcohol steep step optimum extraction condition is: alcohol concn 40-80%, solid-liquid ratio is 1:5-15, extracts temperature 30-80 ℃, extraction time 1-4h, extract 1-4 time repeatedly.
4. the extraction process of a kind of Chinese yam oligose as claimed in claim 1, is characterized in that in described membrane sepn step adopting ultra-filtration membrane (5000D) and two kinds of films of nanofiltration membrane (500D) to complete the oligose sepn process.
5. the extraction process of a kind of Chinese yam oligose as claimed in claim 1, is characterized in that collected filtrate is the component of molecular weight between 500D-5000D.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201310423407 CN103467614A (en) | 2013-09-17 | 2013-09-17 | Process for extracting yam oligosaccharide |
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| CN 201310423407 CN103467614A (en) | 2013-09-17 | 2013-09-17 | Process for extracting yam oligosaccharide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104448017A (en) * | 2014-11-27 | 2015-03-25 | 秦富英 | Method for extracting Chinese yam oligosaccharide |
| CN106632528A (en) * | 2016-12-27 | 2017-05-10 | 温县兴发生物科技有限公司 | Preparation method of passion fruit oligosaccharide |
| CN111620957A (en) * | 2020-04-10 | 2020-09-04 | 中国科学院上海药物研究所 | Chinese yam polysaccharide, preparation method and application thereof |
-
2013
- 2013-09-17 CN CN 201310423407 patent/CN103467614A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104448017A (en) * | 2014-11-27 | 2015-03-25 | 秦富英 | Method for extracting Chinese yam oligosaccharide |
| CN106632528A (en) * | 2016-12-27 | 2017-05-10 | 温县兴发生物科技有限公司 | Preparation method of passion fruit oligosaccharide |
| CN111620957A (en) * | 2020-04-10 | 2020-09-04 | 中国科学院上海药物研究所 | Chinese yam polysaccharide, preparation method and application thereof |
| CN111620957B (en) * | 2020-04-10 | 2021-12-10 | 中国科学院上海药物研究所 | Chinese yam polysaccharide, preparation method and application thereof |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20131225 |
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| WD01 | Invention patent application deemed withdrawn after publication |