CN105412613A - Composition for Alzheimer's disease and processing method of composition - Google Patents
Composition for Alzheimer's disease and processing method of composition Download PDFInfo
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Abstract
The invention discloses a composition for the Alzheimer's disease, and further discloses a processing method of the composition. The processing method comprises the following steps: preparing huperzia serrata coarse powder from huperzia serrata plant; preparing dendrobium candidum coarse powder from dendrobium candidum; preparing lycium barbarum coarse powder from lycium barbarum; preparing anthocyanin coarse powder from purple sweet potato; preparing the obtained huperzia serrata coarse powder, dendrobium candidum coarse powder, lycium barbarum coarse powder and anthocyanin coarse powder into tablets or capsules according to proportion. Polysaccharide and dendrobine of dendrobium candidum, huperzia serrata of huperzia serrata, polysaccharide and isoflavone of lycium barbarum and anthocyanin are scientifically selected for the first time, so as to develop the health product for obviously preventing and treating the Alzheimer's disease. The production process is simple, the cost is low, and the product with obvious curative effects can be obtained without purification of the chemical components. The composition is obvious and stable in curative effects on the Alzheimer's disease, and the relapse is avoided within 1-2 years after drug withdrawal.
Description
Technical field
The invention belongs to senile brain-tonifying health-care production technical field, be specifically related to a kind of formula for senile dementia, also relate to a kind of processing method of the formula for senile dementia.
Background technology
Herba Lycopodii serrati is Herba Lycopodii serrati again, the huperzine A contained has the activity of extremely significant acetylcholine esterase inhibition, reach the effect strengthening Neurotransmission, there is significant memory reinforcing clinically, alleviate senile dementia, be used to senile dementia special for treating medicine.
Herba Dendrobii Dendrobiumcandidum is the orchid family Orchidaceae Dendrobium DendrobiumSw. integration of edible and medicinal herbs perennial meat draft Epiphyte, first of China's nine large Herba mesonae chinensis, containing dendrobium polysaccharide, dendrobine, 6-hydroxydendrobine, Herba Dendrobii time alkali, several amino acids and trace element, wherein polyoses content is up to 22.7%.There is effect of strengthening by means of tonics, be particularly useful for senile having a delicate constitution, in reduction blood glucose, blood fat reducing, suppression atherosclerosis, antitumor and slow down aging etc., there is extremely important medical value.
Lycium barbarum polysaccharide is best suited for the vegetable polysaccharides supplemented in human body polysaccharide, is the first-class medical material Chinese medicine composition of Chinese Pharmacopoeia regulation, to promoting that the stable of structure and fuction of human-body biological film has extremely important effect.Fructus Lycii isoflavone is multiple micromolecule isoflavone like substance, depressed is absorbed and intercellular transport by the intestines and stomach, and bioavailability is high, to removing thrombosis and atherosis effect remarkable.
Anthocyanidin is generally acknowledged oxidation-resistant active ingredient, can hypnotic, slow down aging, especially has remarkable efficacy for alleviation cerebral oxidative stress.
Senile dementia clinical manifestation is that Acetylcholinesterasein strengthens, and Neurotransmission weakens, thus causes senile dementia.But, senile dementia is a kind of result of comprehensive aging, comprise vivo oxidation pressure to strengthen, cause peroxidation of cellular membranes to such an extent as to be combined with acetylcholine declining with signal transduction ability, neuronal cell secretion signal molecule---the function reduction of acetylcholine, etc., comprehensively cause senile dementia.By alleviating cerebral oxidative stress, promote that biomembranous structure and fuction is stable and suppress cholinesterase activity simultaneously, be the effective ways of Comprehensive Treatment senile dementia.Therefore, use promote the lycium barbarum polysaccharide of biofilm structure and function-stable and dendrobium polysaccharide, removing blood stasis Fructus Lycii isoflavone, reduce oxidative pressure anthocyanidin and suppress the first-class composition of the huperzine of acetylcholine esterase comprehensively to use, effectively and stably can alleviate senile dementia.
Summary of the invention
The object of the invention is to overcome the above-mentioned defect existed in prior art, a kind of formula for senile dementia is provided, a kind of processing method of the formula for senile dementia is also provided.
In order to realize above-mentioned object, the present invention adopts following technical measures:
For a formula for senile dementia, comprise the raw material of following mass parts:
A kind of step of processing method of the formula for senile dementia:
The first step: Herba Lycopodii serrati plant is dried and pulverizes, be that 80-95% ethanol boils and boils filtration by the concentration of Herba Lycopodii serrati plant 5-10 times of quality, evaporate to dryness filtrate obtains Herba Lycopodii serrati ethanol extract, and Herba Lycopodii serrati ethanol extract oven dry pulverizing is obtained the Herba Lycopodii serrati coarse powder that huperzine A content is about 1-3%;
Second step: Herba Dendrobii is dried and pulverized, and filter with the decocting in water of Herba Dendrobii 5-10 times quality, evaporate to dryness filtrate obtains Herba Dendrobii aqueous extract; The concentration of the residual residue residual residue 5-10 times quality obtained after filtration is that 80-95% ethanol boils and boils, then filter and by filtrate evaporate to dryness, obtain Herba Dendrobii ethanol extract, in Herba Dendrobii ethanol extract, the content of dendrobine is 5-10%, Herba Dendrobii aqueous extract and Herba Dendrobii ethanol extract are combined dry and pulverize, obtain Herba Dendrobii coarse powder;
3rd step: Lycium barbarum L. is dried and pulverized, filter with after the decocting in water of Lycium barbarum L. 5-10 times quality, filtrate after decocting in water filters is concentrated to pulpous state, add extraction into ethyl acetate and obtain acetic acid ethyl acetate extract, acetic acid ethyl acetate extract obtains Lycium barbarum L. extractum and dries pulverizing after reclaiming ethyl acetate, obtain Fructus Lycii isoflavone powder, in Fructus Lycii isoflavone powder, rutin meter isoflavone content is at 30-50%; The ethanol that filtering residue after Lycium barbarum L. decocting in water filters adds 95-100% concentration 80%, staticly settles 5-24 hour to ethanol final concentration, and collecting precipitation is dried and obtained Fructus Lycii crude polysaccharides, Fructus Lycii isoflavone powder and lycium barbarum polysaccharide is combined and obtains Fructus Lycii coarse powder;
4th step: Rhizoma Steudnerae Henryanae adds water making beating, Rhizoma Steudnerae Henryanae: the mass ratio of water is 1:5-10, obtains serosity, serosity leaves standstill, and collects the supernatant, and by the supernatant, concentrated and evaporate to dryness is pulverized, and obtains anthocyanidin coarse powder;
5th step: the Herba Lycopodii serrati coarse powder, Herba Dendrobii coarse powder, Fructus Lycii coarse powder and the anthocyanidin coarse powder that obtain are made tablet or capsule according to following raw material mass mixture ratio:
Or a kind of formula for senile dementia, comprises following raw material composition:
A kind of step of processing method of the formula for senile dementia is as follows:
Step 1, Herba Lycopodii serrati, Herba Dendrobii, Lycium barbarum L. and Rhizoma Steudnerae Henryanae are shredded oven dry respectively, the Herba Lycopodii serrati, Herba Dendrobii, Lycium barbarum L. and the Rhizoma Steudnerae Henryanae that take according to the mass fraction ratio of following raw material dry weight:
Step 2, by taking Herba Lycopodii serrati, Herba Dendrobii, Lycium barbarum L. and Rhizoma Steudnerae Henryanae mixing, pulverize obtain raw mixture,
Step 3, then at the temperature of 30 ~ 60 degrees Celsius, invade bubble more than 10 hours with the ethanol that the concentration of raw mixture 5 ~ 10 times of quality is 70 ~ 95%, or be put into 30 ~ 60 degrees Celsius after the ethanol mixing of 70 ~ 95% and 1 ~ 5 atmospheric pressurized tank lixiviate 5 ~ 24 hours in the concentration of raw mixture and raw mixture 5 ~ 10 times of quality
Step 4, then to filter, obtain extracting solution and filtering residue,
Step 5, extracting solution is concentrated evaporate to dryness, dries and pulverize, obtain total ethanol extract extractum,
Step 6, filtering residue is boiled with 5 ~ 10 times of quality pure water boil 3 ~ 5 hours again, at the moisture supplementing in the process of boiling and evaporate that boils, then filter and obtain filtrate, filtrate is obtained aqueous extract 60-80 degree Celsius of lower evaporated under reduced pressure, aqueous extract is dried and pulverizes, obtain water total extract.Total ethanol extract extractum and water total extract are merged, is made into tablet or capsule.
The present invention has the following advantages compared with traditional product for invigorating function of brain:
(1) science selects polysaccharide and dendrobine, the Herba Lycopodii serrati of Herba Lycopodii serrati, the polysaccharide of Fructus Lycii and isoflavone, the anthocyanidin of Herba Dendrobii first, develops the health promoting product with significant prevention and therapy senile dementia.
(2) production technology is simple, with low cost, without the need to chemical composition purification, can obtain the product of significant curative effect
(3) this formula is evident in efficacy and stable to senile dementia, can not rebound after drug withdrawal in 1-2.
Detailed description of the invention
Embodiment 1:
For a processing method for the formula of senile dementia, comprise the following steps:
The first step: Herba Lycopodii serrati plant is dried and pulverizes, be that 80% ethanol boils and boils filtration by the concentration of Herba Lycopodii serrati plant 5 times of quality, evaporate to dryness filtrate obtains Herba Lycopodii serrati ethanol extract, and Herba Lycopodii serrati ethanol extract is dried the Herba Lycopodii serrati coarse powder pulverized and obtain huperzine A content about 13%;
Second step: Herba Dendrobii is dried and pulverized, and filter with the decocting in water of Herba Dendrobii 5 times of quality, evaporate to dryness filtrate obtains Herba Dendrobii aqueous extract, and in Herba Dendrobii aqueous extract, dendrobium polysaccharide content is 30%; The concentration of the residual residue residual residue 5 times of quality obtained after filtration is that 80% ethanol boils and boils, then filter and by filtrate evaporate to dryness, obtain Herba Dendrobii ethanol extract, in Herba Dendrobii ethanol extract, the content of dendrobine is 5%, Herba Dendrobii aqueous extract and Herba Dendrobii ethanol extract are combined dry and pulverize, obtain Herba Dendrobii coarse powder, Herba Dendrobii coarse powder main component is dendrobium polysaccharide and dendrobine;
3rd step: Lycium barbarum L. is dried and pulverized, filter with after the decocting in water of Lycium barbarum L. 5 times of quality, filtrate after decocting in water filters is concentrated to pulpous state, add extraction into ethyl acetate and obtain acetic acid ethyl acetate extract, acetic acid ethyl acetate extract obtains Lycium barbarum L. extractum and dries pulverizing after reclaiming ethyl acetate, obtain Fructus Lycii isoflavone powder, in Fructus Lycii isoflavone powder, rutin meter isoflavone content is 30%; The ethanol that filtering residue after Lycium barbarum L. decocting in water filters adds 95% concentration to ethanol final concentration 80%, staticly settle 5 hours, collecting precipitation is dried and is obtained Fructus Lycii crude polysaccharides, in Fructus Lycii crude polysaccharides, glucose meter polyoses content is 20%, Fructus Lycii isoflavone powder and lycium barbarum polysaccharide is combined and obtains Fructus Lycii coarse powder;
4th step: Rhizoma Steudnerae Henryanae adds water making beating, Rhizoma Steudnerae Henryanae: the mass ratio of water is 1:5, obtains serosity, serosity leaves standstill, and collects the supernatant, and by the supernatant, concentrated and evaporate to dryness is pulverized, and obtains anthocyanidin coarse powder;
5th step: the Herba Lycopodii serrati coarse powder, Herba Dendrobii coarse powder, Fructus Lycii coarse powder and the anthocyanidin coarse powder that obtain are made tablet according to following raw material mass mixture ratio:
Senile dementia symptom be can significantly alleviate after patients of senile dementia takes the present embodiment prescription drug, memory and muscle power improved.
Adopt 24 monthly age aged mouses, perfusion scopolamine induction senile dementia chemical model mice, and then gavage the present embodiment prescription drug, adopt maze method to compare mice search of food before and after the medication time used, test main result is as following table:
Table 1 mice walks maze test result (test number (TN) N=5, results averaged)
From upper table result, walk labyrinth Time transfer receiver used after aged dementia mice medication and significantly reduce according to group, illustrate that the formula of the present embodiment has significant curative effect to aged dementia mice.
In order to determine the comprehensive medicinal efficacy of this formula further, adopt 24 monthly age these prescription drugs of aged mouse gavage, then analyzed the medicinal efficacy of this formula by test mice serum reduction-state level (DPPH method) and anti-fatigue ability (swimming time), the results are shown in Table 2:
The serum reduction-state of the male Mus of table 2 and anti-fatigue test (test number (TN) N=5)
| Serum is to free radical (DPPH) clearance rate (%) | Swimming time (min) | |
| Matched group | 29.5±8.3 | 25±8 |
| Administration group | 46.5±11.5 | 44±11 |
As can be seen from Table 2, after taking the present embodiment prescription drug, the general health of mice is significantly improved, and shows that serum reduction-state and anti-fatigue ability strengthen greatly.Therefore, the present embodiment formula not only improves the ability of learning and memory of mice, greatly can also improve the health level of aged mouse.
Embodiment 2:
The invention discloses a kind of processing method of the formula for senile dementia.Its formula comprises: Herba Lycopodii serrati ethanol extract, Herba Dendrobii aqueous extract and ethanol extract, lycium barbarum polysaccharide isoflavone and anthocyanidin powder.A kind of for the formula of senile dementia and the step of processing method thereof:
The first step: Herba Lycopodii serrati plant is dried and pulverizes, be that 90% ethanol boils and boils filtration by the concentration of Herba Lycopodii serrati plant 7 times of quality, evaporate to dryness filtrate obtains Herba Lycopodii serrati ethanol extract, and Herba Lycopodii serrati ethanol extract is dried the Herba Lycopodii serrati coarse powder pulverized and obtain huperzine A content about 2%;
Second step: Herba Dendrobii is dried and pulverized, and filter with the decocting in water of Herba Dendrobii 7 times of quality, evaporate to dryness filtrate obtains Herba Dendrobii aqueous extract, and in Herba Dendrobii aqueous extract, dendrobium polysaccharide content is 40%; The concentration of the residual residue residual residue 7 times of quality obtained after filtration is that 90% ethanol boils and boils, then filter and by filtrate evaporate to dryness, obtain Herba Dendrobii ethanol extract, in Herba Dendrobii ethanol extract, the content of dendrobine is 7%, Herba Dendrobii aqueous extract and Herba Dendrobii ethanol extract are combined dry and pulverize, obtain Herba Dendrobii coarse powder, Herba Dendrobii coarse powder main component is dendrobium polysaccharide and dendrobine;
3rd step: Lycium barbarum L. is dried and pulverized, filter with after the decocting in water of Lycium barbarum L. 7 times of quality, filtrate after decocting in water filters is concentrated to pulpous state, add extraction into ethyl acetate and obtain acetic acid ethyl acetate extract, acetic acid ethyl acetate extract obtains Lycium barbarum L. extractum and dries pulverizing after reclaiming ethyl acetate, obtain Fructus Lycii isoflavone powder, in Fructus Lycii isoflavone powder, rutin meter isoflavone content is 40%; The ethanol that filtering residue after Lycium barbarum L. decocting in water filters adds 97% concentration to ethanol final concentration 80%, staticly settle 18 hours, collecting precipitation is dried and is obtained Fructus Lycii crude polysaccharides, in Fructus Lycii crude polysaccharides, glucose meter polyoses content is 26%, Fructus Lycii isoflavone powder and lycium barbarum polysaccharide is combined and obtains Fructus Lycii coarse powder;
4th step: Rhizoma Steudnerae Henryanae adds water making beating, Rhizoma Steudnerae Henryanae: the mass ratio of water is 1:7, obtains serosity, serosity leaves standstill, and collects the supernatant, and by the supernatant, concentrated and evaporate to dryness is pulverized, and obtains anthocyanidin coarse powder;
5th step: the Herba Lycopodii serrati coarse powder, Herba Dendrobii coarse powder, Fructus Lycii coarse powder and the anthocyanidin coarse powder that obtain are made tablet or capsule according to following raw material mass mixture ratio:
Senile dementia symptom be can significantly alleviate after patients of senile dementia takes the present embodiment prescription drug, memory and muscle power improved.
Adopt 24 monthly age aged mouses, perfusion scopolamine induction senile dementia chemical model mice, and then gavage the present embodiment prescription drug, adopt maze method to compare mice search of food before and after the medication time used, test main result is as following table:
Table 3 mice walks maze test result (test number (TN) N=5, results averaged)
From upper table result, walk labyrinth Time transfer receiver used after aged dementia mice medication and significantly reduce according to group, illustrate that the formula of the present embodiment has significant curative effect to aged dementia mice.
In order to determine the comprehensive medicinal efficacy of this formula further, adopt 24 monthly age these prescription drugs of aged mouse gavage, then analyzed the medicinal efficacy of this formula by test mice serum reduction-state level (DPPH method) and anti-fatigue ability (swimming time), the results are shown in Table 4:
The serum reduction-state of the male Mus of table 4 and anti-fatigue test (test number (TN) N=5)
| Serum is to free radical (DPPH) clearance rate (%) | Swimming time (min) | |
| Matched group | 32.5±9.7 | 28±5 |
| Administration group | 47.9±10.2 | 42±8 |
As can be seen from Table 4, after taking prescription drug of the present invention, the general health of mice is significantly improved, and shows that serum reduction-state and anti-fatigue ability strengthen greatly.Therefore, formula of the present invention not only improves the ability of learning and memory of mice, greatly can also improve the health level of aged mouse.
Embodiment 3:
For a processing method for the formula of senile dementia, comprise the following steps:
The first step: Herba Lycopodii serrati plant is dried and pulverizes, be that 95% ethanol boils and boils filtration by the concentration of Herba Lycopodii serrati plant 10 times of quality, evaporate to dryness filtrate obtains Herba Lycopodii serrati ethanol extract, and Herba Lycopodii serrati ethanol extract is dried the Herba Lycopodii serrati coarse powder pulverized and obtain huperzine A content about 3%;
Second step: Herba Dendrobii is dried and pulverized, and filter with the decocting in water of Herba Dendrobii 10 times of quality, evaporate to dryness filtrate obtains Herba Dendrobii aqueous extract, and in Herba Dendrobii aqueous extract, dendrobium polysaccharide content is 50%; The concentration of the residual residue residual residue 10 times of quality obtained after filtration is that 95% ethanol boils and boils, then filter and by filtrate evaporate to dryness, obtain Herba Dendrobii ethanol extract, in Herba Dendrobii ethanol extract, the content of dendrobine is 10%, Herba Dendrobii aqueous extract and Herba Dendrobii ethanol extract are combined dry and pulverize, obtain Herba Dendrobii coarse powder, Herba Dendrobii coarse powder main component is dendrobium polysaccharide and dendrobine;
3rd step: Lycium barbarum L. is dried and pulverized, filter with after the decocting in water of Lycium barbarum L. 10 times of quality, filtrate after decocting in water filters is concentrated to pulpous state, add extraction into ethyl acetate and obtain acetic acid ethyl acetate extract, acetic acid ethyl acetate extract obtains Lycium barbarum L. extractum and dries pulverizing after reclaiming ethyl acetate, obtain Fructus Lycii isoflavone powder, in Fructus Lycii isoflavone powder, rutin meter isoflavone content is 50%; The ethanol that filtering residue after Lycium barbarum L. decocting in water filters adds 100% concentration to ethanol final concentration 80%, staticly settle 24 hours, collecting precipitation is dried and is obtained Fructus Lycii crude polysaccharides, in Fructus Lycii crude polysaccharides, glucose meter polyoses content is 30%, Fructus Lycii isoflavone powder and lycium barbarum polysaccharide is combined and obtains Fructus Lycii coarse powder;
4th step: Rhizoma Steudnerae Henryanae adds water making beating, Rhizoma Steudnerae Henryanae: the mass ratio of water is 1:10, obtains serosity, serosity leaves standstill, and collects the supernatant, and by the supernatant, concentrated and evaporate to dryness is pulverized, and obtains anthocyanidin coarse powder;
5th step: the Herba Lycopodii serrati coarse powder, Herba Dendrobii coarse powder, Fructus Lycii coarse powder and the anthocyanidin coarse powder that obtain are made tablet or capsule according to following raw material mass mixture ratio:
Senile dementia symptom be can significantly alleviate after patients of senile dementia takes the present embodiment prescription drug, memory and muscle power improved.
Adopt 24 monthly age aged mouses, perfusion scopolamine induction senile dementia chemical model mice, and then gavage the present embodiment prescription drug, adopt maze method to compare mice search of food before and after the medication time used, test main result is as following table:
Table 5 mice walks maze test result (test number (TN) N=5, results averaged)
From upper table result, walk labyrinth Time transfer receiver used after aged dementia mice medication and significantly reduce according to group, illustrate that the formula of the present embodiment has significant curative effect to aged dementia mice.
In order to determine the comprehensive medicinal efficacy of this formula further, adopt 24 monthly age these prescription drugs of aged mouse gavage, then analyzed the medicinal efficacy of this formula by test mice serum reduction-state level (DPPH method) and anti-fatigue ability (swimming time), the results are shown in Table 6:
The serum reduction-state of the male Mus of table 6 and anti-fatigue test (test number (TN) N=5)
| Serum is to free radical (DPPH) clearance rate (%) | Swimming time (min) | |
| Matched group | 32.6±9.2 | 28±5 |
| Administration group | 51.5±9.5 | 50±7 |
As can be seen from Table 6, after taking prescription drug of the present invention, the general health of mice is significantly improved, and shows that serum reduction-state and anti-fatigue ability strengthen greatly.Therefore, formula of the present invention not only improves the ability of learning and memory of mice, greatly can also improve the health level of aged mouse.
Embodiment 4:
For a processing method for the formula of senile dementia, comprise the following steps:
Step 1, Herba Lycopodii serrati, Herba Dendrobii, Lycium barbarum L. and Rhizoma Steudnerae Henryanae are shredded oven dry respectively, the Herba Lycopodii serrati, Herba Dendrobii, Lycium barbarum L. and the Rhizoma Steudnerae Henryanae that take according to the mass fraction ratio of following raw material dry weight:
Step 2, by taking Herba Lycopodii serrati, Herba Dendrobii, Lycium barbarum L. and Rhizoma Steudnerae Henryanae mixing, pulverize obtain raw mixture,
Step 3, then at the temperature of 30 degrees Celsius, invade bubble more than 10 hours with the ethanol that the concentration of raw mixture 5 times of quality is 70%, or be put into 30 degrees Celsius after the ethanol mixing of 70% and 1 atmospheric pressurized tank lixiviate 5 hours in the concentration of raw mixture and raw mixture 5 times of quality
Step 4, then to filter, obtain extracting solution and filtering residue,
Step 5, extracting solution is concentrated evaporate to dryness, dries and pulverize, obtain total ethanol extract extractum,
Step 6, filtering residue boiled with 5 times of quality pure water boil 3 hours again, at the moisture supplementing in the process of boiling and evaporate that boils, then filter and obtain filtrate, by filtrate at 60 c evaporated under reduced pressure obtain aqueous extract, aqueous extract is dried and pulverizes, obtain water total extract.Total ethanol extract extractum and water total extract are merged, is made into tablet or capsule.
Senile dementia symptom be can significantly alleviate after patients of senile dementia takes the present embodiment tablet or capsule, memory and muscle power improved.
Mouse test checking is carried out to the prescription drug that the present embodiment makes: adopt 24 monthly age aged mouses, perfusion scopolamine induction senile dementia chemical model mice, and then gavage prescription drug of the present invention, adopt maze method to compare mice search of food before and after the medication time used, test main result is as following table:
Table 7 mice walks maze test result (test number (TN) N=5, results averaged)
From upper table result, walk labyrinth Time transfer receiver used after aged dementia mice medication and significantly reduce according to group, illustrate that formula of the present invention has significant curative effect to aged dementia mice.
In order to determine the comprehensive medicinal efficacy of the present embodiment formula further, adopt 24 monthly age these prescription drugs of aged mouse gavage, then analyzed the medicinal efficacy of this formula by test mice serum reduction-state level (DPPH method) and anti-fatigue ability (swimming time), the results are shown in Table 8:
The serum reduction-state of the male Mus of table 8 and anti-fatigue test (test number (TN) N=5)
| Serum is to free radical (DPPH) clearance rate (%) | Swimming time | |
| Matched group | 31.6±10.5 | 32±10 |
| Administration group | 50.5±12.5 | 68±15 |
As can be seen from Table 8, after taking the present embodiment prescription drug, the general health of mice is significantly improved, and shows that serum reduction-state and anti-fatigue ability strengthen greatly.Therefore, this formula not only improves the ability of learning and memory of mice, greatly can also improve the health level of aged mouse.
Embodiment 5:
For a processing method for the formula of senile dementia, comprise the following steps:
Step 1, Herba Lycopodii serrati, Herba Dendrobii, Lycium barbarum L. and Rhizoma Steudnerae Henryanae are shredded oven dry respectively, the Herba Lycopodii serrati, Herba Dendrobii, Lycium barbarum L. and the Rhizoma Steudnerae Henryanae that take according to the mass fraction ratio of following raw material dry weight:
Step 2, by taking Herba Lycopodii serrati, Herba Dendrobii, Lycium barbarum L. and Rhizoma Steudnerae Henryanae mixing, pulverize obtain raw mixture,
Step 3, then at the temperature of 33 degrees Celsius, invade bubble more than 10 hours with the ethanol that the concentration of raw mixture 7 times of quality is 86%, or be put into 46 degrees Celsius after the ethanol mixing of 81% and 3 atmospheric pressurized tank lixiviates 15 hours in the concentration of raw mixture and raw mixture 7 times of quality
Step 4, then to filter, obtain extracting solution and filtering residue,
Step 5, extracting solution is concentrated evaporate to dryness, dries and pulverize, obtain total ethanol extract extractum,
Step 6, filtering residue is boiled with 7 times of quality pure water boil 4 hours again, at the moisture supplementing in the process of boiling and evaporate that boils, then filter and obtain filtrate, filtrate is obtained aqueous extract 70 degrees Celsius of lower evaporated under reduced pressure, aqueous extract is dried and pulverizes, obtain water total extract.Total ethanol extract extractum and water total extract are merged, is made into tablet or capsule.
Senile dementia symptom be can significantly alleviate after patients of senile dementia takes the present embodiment tablet or capsule, memory and muscle power improved.
Mouse test checking is carried out to the prescription drug that the present embodiment makes: adopt 24 monthly age aged mouses, perfusion scopolamine induction senile dementia chemical model mice, and then gavage prescription drug of the present invention, adopt maze method to compare mice search of food before and after the medication time used, test main result is as following table:
Table 9 mice walks maze test result (test number (TN) N=5, results averaged)
From upper table result, walk labyrinth Time transfer receiver used after aged dementia mice medication and significantly reduce according to group, illustrate that formula of the present invention has significant curative effect to aged dementia mice.
In order to determine the comprehensive medicinal efficacy of the present embodiment formula further, adopt 24 monthly age these prescription drugs of aged mouse gavage, then analyzed the medicinal efficacy of this formula by test mice serum reduction-state level (DPPH method) and anti-fatigue ability (swimming time), the results are shown in Table 10:
The serum reduction-state of the male Mus of table 10 and anti-fatigue test (test number (TN) N=5)
| Serum is to free radical (DPPH) clearance rate (%) | Swimming time | |
| Matched group | 36.6±8.5 | 30±8 |
| Administration group | 48.5±9.3 | 51±15 |
As can be seen from Table 10, after taking the present embodiment prescription drug, the general health of mice is significantly improved, and shows that serum reduction-state and anti-fatigue ability strengthen greatly.Therefore, this formula not only improves the ability of learning and memory of mice, greatly can also improve the health level of aged mouse.
Embodiment 6: a kind of processing method of the formula for senile dementia, comprises the following steps:
Step 1, Herba Lycopodii serrati, Herba Dendrobii, Lycium barbarum L. and Rhizoma Steudnerae Henryanae are shredded oven dry respectively, the Herba Lycopodii serrati, Herba Dendrobii, Lycium barbarum L. and the Rhizoma Steudnerae Henryanae that take according to the mass fraction ratio of following raw material dry weight:
Step 2, by taking Herba Lycopodii serrati, Herba Dendrobii, Lycium barbarum L. and Rhizoma Steudnerae Henryanae mixing, pulverize obtain raw mixture,
Step 3, then invade bubble at a temperature of 60 degrees c more than 10 hours with the ethanol that the concentration of raw mixture 10 times of quality is 95%, or be put into 60 degrees Celsius after the ethanol mixing of 95% and 5 atmospheric pressurized tank lixiviates 24 hours in the concentration of raw mixture and raw mixture 10 times of quality
Step 4, then to filter, obtain extracting solution and filtering residue,
Step 5, extracting solution is concentrated evaporate to dryness, dries and pulverize, obtain total ethanol extract extractum,
Step 6, filtering residue boiled with 10 times of quality pure water boil 5 hours again, at the moisture supplementing in the process of boiling and evaporate that boils, then filter and obtain filtrate, by filtrate at 80 degrees celsius evaporated under reduced pressure obtain aqueous extract, aqueous extract is dried and pulverizes, obtain water total extract.Total ethanol extract extractum and water total extract are merged, is made into tablet or capsule.
Senile dementia symptom be can significantly alleviate after patients of senile dementia takes the present embodiment tablet or capsule, memory and muscle power improved.
Mouse test checking is carried out to the prescription drug that the present embodiment makes: adopt 24 monthly age aged mouses, perfusion scopolamine induction senile dementia chemical model mice, and then gavage prescription drug of the present invention, adopt maze method to compare mice search of food before and after the medication time used, test main result is as following table:
Table 11 mice walks maze test result (test number (TN) N=5, results averaged)
From upper table result, walk labyrinth Time transfer receiver used after aged dementia mice medication and significantly reduce according to group, illustrate that formula of the present invention has significant curative effect to aged dementia mice.
In order to determine the comprehensive medicinal efficacy of the present embodiment formula further, adopt 24 monthly age these prescription drugs of aged mouse gavage, then analyzed the medicinal efficacy of this formula by test mice serum reduction-state level (DPPH method) and anti-fatigue ability (swimming time), the results are shown in Table 12:
The serum reduction-state of the male Mus of table 12 and anti-fatigue test (test number (TN) N=5)
| Serum is to free radical (DPPH) clearance rate (%) | Swimming time | |
| Matched group | 38.8±11.3 | 30±5 |
| Administration group | 55.5±15.5 | 57±11 |
As can be seen from Table 12, after taking the present embodiment prescription drug, the general health of mice is significantly improved, and shows that serum reduction-state and anti-fatigue ability strengthen greatly.Therefore, this formula not only improves the ability of learning and memory of mice, greatly can also improve the health level of aged mouse.
Specific embodiment described herein is only to the explanation for example of the present invention's spirit.Those skilled in the art can make various amendment or supplement or adopt similar mode to substitute to described specific embodiment, but can't depart from spirit of the present invention or surmount the scope that appended claims defines.
Claims (2)
1. for a formula for senile dementia, it is characterized in that, comprise the raw material of following mass parts:
Raw materials quality part
Herba Lycopodii serrati coarse powder 40 ~ 70
Herba Dendrobii coarse powder 10 ~ 20
Fructus Lycii coarse powder 10 ~ 30
Anthocyanidin coarse powder 5 ~ 10.
2. for a processing method for the formula of senile dementia, it is characterized in that, comprise the following steps:
The first step: Herba Lycopodii serrati plant is dried and pulverizes, be that 80-95% ethanol boils and boils filtration by the concentration of Herba Lycopodii serrati plant 5-10 times of quality, evaporate to dryness filtrate obtains Herba Lycopodii serrati ethanol extract, and Herba Lycopodii serrati ethanol extract oven dry pulverizing is obtained the Herba Lycopodii serrati coarse powder that huperzine A content is about 1-3%;
Second step: Herba Dendrobii is dried and pulverized, and filter with the decocting in water of Herba Dendrobii 5-10 times quality, evaporate to dryness filtrate obtains Herba Dendrobii aqueous extract; The concentration of the residual residue residual residue 5-10 times quality obtained after filtration is that 80-95% ethanol boils and boils, then filter and by filtrate evaporate to dryness, obtain Herba Dendrobii ethanol extract, in Herba Dendrobii ethanol extract, the content of dendrobine is 5-10%, Herba Dendrobii aqueous extract and Herba Dendrobii ethanol extract are combined dry and pulverize, obtain Herba Dendrobii coarse powder;
3rd step: Lycium barbarum L. is dried and pulverized, filter with after the decocting in water of Lycium barbarum L. 5-10 times quality, filtrate after decocting in water filters is concentrated to pulpous state, add extraction into ethyl acetate and obtain acetic acid ethyl acetate extract, acetic acid ethyl acetate extract obtains Lycium barbarum L. extractum and dries pulverizing after reclaiming ethyl acetate, obtain Fructus Lycii isoflavone powder, in Fructus Lycii isoflavone powder, rutin meter isoflavone content is at 30-50%; The ethanol that filtering residue after Lycium barbarum L. decocting in water filters adds 95-100% concentration 80%, staticly settles 5-24 hour to ethanol final concentration, and collecting precipitation is dried and obtained Fructus Lycii crude polysaccharides, Fructus Lycii isoflavone powder and lycium barbarum polysaccharide is combined and obtains Fructus Lycii coarse powder;
4th step: Rhizoma Steudnerae Henryanae adds water making beating, Rhizoma Steudnerae Henryanae: the mass ratio of water is 1:5-10, obtains serosity, serosity leaves standstill, and collects the supernatant, and by the supernatant, concentrated and evaporate to dryness is pulverized, and obtains anthocyanidin coarse powder;
5th step: the Herba Lycopodii serrati coarse powder, Herba Dendrobii coarse powder, Fructus Lycii coarse powder and the anthocyanidin coarse powder that obtain are made tablet or capsule according to following raw material mass mixture ratio:
Raw materials quality part
Herba Lycopodii serrati coarse powder 40 ~ 70
Herba Dendrobii coarse powder 10 ~ 20
Fructus Lycii coarse powder 10 ~ 30
Anthocyanidin coarse powder 5 ~ 10.
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