CN104770300B - A kind of method of fast breeding wool savatier monochasma herb seedling - Google Patents
A kind of method of fast breeding wool savatier monochasma herb seedling Download PDFInfo
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- CN104770300B CN104770300B CN201510183045.1A CN201510183045A CN104770300B CN 104770300 B CN104770300 B CN 104770300B CN 201510183045 A CN201510183045 A CN 201510183045A CN 104770300 B CN104770300 B CN 104770300B
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- Prior art keywords
- seedling
- savatier monochasma
- monochasma herb
- culture medium
- wool
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- 210000002268 wool Anatomy 0.000 title claims abstract description 29
- 241000155182 Monochasma Species 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 15
- 238000009395 breeding Methods 0.000 title claims abstract description 9
- 230000001488 breeding effect Effects 0.000 title claims abstract description 9
- 239000001963 growth medium Substances 0.000 claims abstract description 36
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000005286 illumination Methods 0.000 claims description 10
- 239000002775 capsule Substances 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 4
- 239000005720 sucrose Substances 0.000 claims description 4
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 239000005556 hormone Substances 0.000 abstract description 2
- 229940088597 hormone Drugs 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 230000008117 seed development Effects 0.000 abstract 1
- 239000002689 soil Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 244000025254 Cannabis sativa Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 210000003056 antler Anatomy 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 239000005648 plant growth regulator Substances 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000013557 Plantaginaceae Species 0.000 description 1
- 238000009412 basement excavation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Abstract
The invention discloses a kind of method of fast breeding wool savatier monochasma herb seedling.Ratio and dosage that the method passes through two kinds of hormones in rational allocation culture medium, while adding the auxiliary materials such as activated carbon so that wool savatier monochasma herb seed development seedling, after seedling replanting to soil is planted, survival rate more than 95%.The method can large scale cultivating wool savatier monochasma herb seedling within a short period of time, meet Production requirement, it is economic and practical, greatly improve culture efficiency, work well, can be used as the important method of wool savatier monochasma herb seedling factorial praluction.
Description
Technical field
The present invention relates to wool savatier monochasma herb factorial praluction technical field, more particularly to a kind of fast breeding wool savatier monochasma herb
The method of seedling.
Background technology
Wool savatier monochasma herb is that Scrophulariaceae savatier monochasma herb belongs to herbaceos perennial, is born in the sandy mountain region in low mountain and thick grass, tool
The effects such as clearing heat and detoxicating, cooling blood and hemostasis.Because artificial cultivation wool savatier monochasma herb not yet succeeds, wild resource excavation is serious, increasingly
Reduce, it would be highly desirable to carry out artificial breeding, protect wild resource.
The content of the invention
The purpose of the present invention is directed to ever-increasing wool savatier monochasma herb demand and the not enough contradiction of wild resource, there is provided one
The method for planting fast breeding wool savatier monochasma herb seedling.
The purpose of the present invention is achieved through the following technical solutions：A kind of side of wool savatier monochasma herb seedling fast breeding
The method of method, the method is：After the seed of wool savatier monochasma herb capsule is done to break in the seed coat, it is placed in culture medium and cultivates, condition of culture
For：25 DEG C of temperature, first cultivates 10-20 days under dark condition, is then cultivated 50-60 days under illumination condition, and illumination condition is：
Daily illumination 12 hours, intensity of illumination 1500-2000lx obtains height of seedling 3-4 cm, has more than the 5 wool savatier monochasma herb childrens of root system
Seedling；The culture medium is：The culture medium based on B5, the 6-benzyl aminopurine containing 0.1mg, 0.1mg in every liter of B5 medium
NAA, the activated carbon of 2.0g, the sucrose of the agar of 3.3g and 30g, pH is 6.0.
The beneficial effects of the invention are as follows：The present invention carries out tissue cultures by staving wool pilose antler grass seed kind skin, and
The addition of rationally control two kinds of hormone dosages and mutual proportioning, and other auxiliary materials, promotes wool pilose antler grass seed to pass through
After callus phase, seedling is continued to grow into, in the case of wool savatier monochasma herb seed source is very rare, greatly improved numerous
Grow efficiency.Result shows that the method meets needed for production, and the important sources of seedling can be produced as wool savatier monochasma herb.
Specific embodiment
A kind of method of fast breeding wool savatier monochasma herb seedling of the present invention, comprises the following steps：
（1）Prepare 12 culture mediums：The culture medium based on B5, in every liter of B5 medium add 6-benzyl aminopurine, α-
Methyl α-naphthyl acetate, activated carbon, agar and sucrose（The amount of each composition is as shown in table 1）, boiling is heated to, stirred in heating process, treat
After agar is completely dissolved, regulation pH value obtains culture medium to 6.0；Equably seedling culture medium is sub-packed in culture vessel, side point
Rim is stirred, it is to avoid activated carbon sinks to the bottom, and packing is added a cover after finishing；After culture vessel is added a cover, sterilizing in high-pressure sterilizing pot is put into, gone out
Bacterium condition is：Sterilized 20 minutes at 121 DEG C, culture vessel is taken out after sterilizing, it is standby after cooling；
| 6-benzyl aminopurine (mg/L) | NAA (mg/L) | Activated carbon (g/L) | Agar (g/L) | Sucrose (g/L) | B5 medium | |
| Culture medium 1 | 0 | 0 | 2 | 3.3 | 30 | 1L |
| Culture medium 2 | 0 | 0.1 | 2 | 3.3 | 30 | 1L |
| Culture medium 3 | 0 | 0.2 | 2 | 3.3 | 30 | 1L |
| Culture medium 4 | 0.1 | 0 | 2 | 3.3 | 30 | 1L |
| Culture medium 5 | 0.1 | 0.1 | 2 | 3.3 | 30 | 1L |
| Culture medium 6 | 0.1 | 0.2 | 2 | 3.3 | 30 | 1L |
| Culture medium 7 | 0.2 | 0 | 2 | 3.3 | 30 | 1L |
| Culture medium 8 | 0.2 | 0.1 | 2 | 3.3 | 30 | 1L |
| Culture medium 9 | 0.2 | 0.2 | 2 | 3.3 | 30 | 1L |
| Culture medium 10 | 0.1 | 0 | 1 | 3.3 | 30 | 1L |
| Culture medium 11 | 0.1 | 0.1 | 1 | 3.3 | 30 | 1L |
| Culture medium 12 | 0.1 | 0.2 | 1 | 3.3 | 30 | 1L |
（2）Wool savatier monochasma herb capsule sterilizes：Wool savatier monochasma herb capsule is cleaned up in clear water；Then in aseptic condition
Under, capsule is soaked 1 minute in the ethanol solution that concentration of volume percent is 75%, it is some with sterile water wash after taking-up
Time, place into liquor natrii hypochloritis that concentration of volume percent is 2% and sterilize 10 minutes, with some times of sterile water wash, blot water
It is put into after point standby in sterile petri dish；
（3）Inoculation：Wool savatier monochasma herb capsule epidermis is opened, the seed in capsule is accessed media surface with tweezers, and
Lane is broken in the seed coat, and covers tightly lid；
（4）Culture：The culture vessel that inoculation will be completed moves into culturing room, and cultivation temperature is 25 DEG C.Dark culturing 10-20
My god, after producing callus by Seed inducement, illumination condition is set to daily illumination 12 hours, intensity of illumination 1500-
2000lx, is further cultured for 50-60 days.
The wool savatier monochasma herb seedling for obtaining is cultivated in above-mentioned 12 culture mediums and is respectively labeled as A ~ L, 12 growths of seedling
Situation（Average value）It is as shown in the table.
| Height of seedling（cm） | Root quantity | Root length（cm） | Survival rate (%) | |
| A (culture medium 1) | 0.2 | 1.5 | 0.2 | 10.5 |
| B (culture medium 2) | 2.2 | 3.3 | 0.6 | 60.0 |
| C (culture medium 3) | 1.8 | 3.0 | 0.4 | 50.7 |
| D (culture medium 4) | 2.1 | 3.5 | 0.6 | 58.0 |
| E (culture medium 5) | 3.8 | 5.8 | 1.1 | 96.0 |
| F (culture medium 6) | 3.2 | 5.0 | 1.0 | 93.2 |
| G (culture medium 7) | 1.9 | 3.1 | 0.5 | 59.4 |
| H (culture medium 8) | 3.4 | 4.6 | 0.9 | 86.0 |
| I (culture medium 9) | 3.0 | 4.2 | 0.7 | 80.2 |
| J (culture medium 10) | 2.0 | 3.2 | 0.5 | 51.3 |
| K (culture medium 11) | 3.6 | 5.6 | 1.0 | 95.5 |
| L (culture medium 12) | 3.1 | 4.7 | 0.9 | 93.0 |
It can be seen from above result of the test, the wool savatier monochasma herb seedling E survival rates for obtaining are cultivated on No. 5 culture mediums and is up to
96.0%, average height of seedling 3.8cm, average 5.8 of root quantity, the average 1.1cm of root length, during its effect is 12 kinds of culture mediums
Best.And wool savatier monochasma herb can hardly grow on No. 1 culture medium without plant growth regulator, plant growth is adjusted
Section agent concentration No. 6, No. 9, No. 12 culture effects of culture medium higher are also not so good as No. 5 culture mediums, and this can also confirm " a small amount of plant
Growth regulator can promote plant growth, and excessive concentration on the contrary can Developing restraint." this extensive common recognition.
Claims (1)
1. a kind of method of fast breeding wool savatier monochasma herb seedling, it is characterised in that the method is：By wool savatier monochasma herb capsule
After seed is done to break in the seed coat, it is placed in culture medium and cultivates, condition of culture is：25 DEG C of temperature, first cultivates 10-20 under dark condition
My god, after producing callus by Seed inducement, illumination condition is set to daily illumination 12 hours, intensity of illumination 1500-
2000lx, is further cultured for 50-60 days, obtains height of seedling 3-4 cm, has more than the 5 wool savatier monochasma herb seedling of root system；The culture medium
For：The culture medium based on B5, the 6-benzyl aminopurine containing 0.1mg in every liter of B5 medium, the NAA of 0.1mg,
The sucrose of the activated carbon of 2.0g, the agar of 3.3g and 30g, pH is 6.0.
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| CN201510183045.1A CN104770300B (en) | 2015-04-17 | 2015-04-17 | A kind of method of fast breeding wool savatier monochasma herb seedling |
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| CN104770300A CN104770300A (en) | 2015-07-15 |
| CN104770300B true CN104770300B (en) | 2017-06-23 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109757313B (en) * | 2019-03-12 | 2023-09-26 | 浙江省中药研究所有限公司 | Transplanting method of hairy antler grass |
| CN110495391A (en) * | 2019-07-26 | 2019-11-26 | 中国林业科学研究院亚热带林业实验中心 | A kind of sterile high efficiency seedling cultivating method of savatier monochasma herb |
| CN111247972A (en) * | 2020-03-12 | 2020-06-09 | 惠州市九惠制药股份有限公司 | Harvesting and storing method of savatier salmoides seeds |
| CN111386981B (en) * | 2020-04-10 | 2022-03-18 | 惠州市九惠制药股份有限公司 | Method for planting savatier saururus in pot |
| CN113133389A (en) * | 2021-04-07 | 2021-07-20 | 宜春市炳晨农业科技发展有限公司 | Seedling growing method for savatier monochasma herb |
| CN113261438A (en) * | 2021-06-08 | 2021-08-17 | 中国医学科学院药用植物研究所 | Method for promoting transplanting survival of pilose antler grass stem cutting seedlings |
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| CN101507415B (en) * | 2009-03-26 | 2011-01-05 | 浙江大学 | In-vitro culture method of antlerpilose grass |
| CN103004601B (en) * | 2012-12-23 | 2013-12-04 | 昆明学院 | Quick reproduction method of melasma arvense (Benth.) Hand.-Mazz. through tissue culture |
| CN104221878A (en) * | 2014-10-14 | 2014-12-24 | 南京帝道农业科技有限公司 | Rapid propagation method for suspension cell culture for savatier monochasma herb |
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