CN104620989B - Method for culturing bitter gourd regenerated seedlings - Google Patents
Method for culturing bitter gourd regenerated seedlings Download PDFInfo
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- CN104620989B CN104620989B CN201510080859.2A CN201510080859A CN104620989B CN 104620989 B CN104620989 B CN 104620989B CN 201510080859 A CN201510080859 A CN 201510080859A CN 104620989 B CN104620989 B CN 104620989B
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- balsam pear
- bitter gourd
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Abstract
The invention discloses a method for culturing bitter gourd regenerated seedlings. The method comprises the following steps: by virtue of a tissue culturing technology, under a sterile condition, performing sterile culturing on a bitter gourd material in a culture medium suitable for inducing callus, then regulating the concentration of hormone of a bitter gourd differentiated bud, and growing a new usable regenerated seedling. According to the method, the problem that the bitter gourd variety with overhigh cost of seeds is not favorably popularized is solved. By virtue of the tissue culturing technology, cell tissues of the bitter gourd differentiate new plantlets, so that the problem of overhigh cost of the seeds can be effectively avoided, the purpose of performing large-scale artificial culturing and factorized production under artificial sterile operation and a suitable environment condition is achieved, and the collection and the anti-season production and cultivation of genetic resources of the bitter gourd variety are facilitated.
Description
Technical field
The present invention relates to balsam pear regrowth cultivates field, more particularly to a kind of method cultivating balsam pear regrowth.
Background technology
Balsam pear is a kind of health-care vegetable, cure mainly that heatstroke, hot summer weather polydipsia, heat furuncle, prickly heat be excessive, dysentery, sore swells, conjunctivitis,
The illnesss such as red eye, swell pain, carbuncle swells erysipelas, burn and scald, oliguresis.In addition, the Determination of Vitamin C of balsam pear is very high, there is prevention bad blood
One is contained inside the effect balsam pears such as disease, protection cell membrane, atherosclerosis, raising physical stress ability, cardioprotection
Plant composition, can effectively suppress the canceration of a normal bag and the recovery promoting special change cell, there is certain antitumaous effect.With
Raising to quality requirements, has introduced some Varieties of Balsam Pears costly, but because expensive ordinary citizen can not consume.And
Effectively reduce the seed costs of this kind of Varieties of Balsam Pear under the premise of how preserving germ plasm resource, reach factorial praluction, anti-season is planted
Training, can meet the requirement of masses, be the problem needing to solve.
By tissue culture technique, seed costs can be effectively reduced, reach factorial praluction, off-season cultivation, protect
Deposit germ plasm resource and can improve the breed composition with popular requirement.At present, tissue culture technique quickly grows, and is also should
With one of widest field, highly developed at home.
Content of the invention
Based on the problems of above-mentioned prior art, the present invention provides a kind of method cultivating balsam pear regrowth, can be real
Now reduce seed costs, factory culture and off-season cultivation, increase economic efficiency.
For solving above-mentioned technical problem, the present invention provides a kind of method cultivating balsam pear regrowth, comprises the following steps:
The seed selecting Varieties of Balsam Pear is pre-processed, disinfection after pretreatment, use the kind after disinfecting
Son carries out cultivating aseptic seedling, is cultivated in vitro using cultivating the aseptic seedling obtaining, the cell tissue of balsam pear is placed in and is suitable for luring
Lead in callus and the culture medium of Bud Differentiation and cultivate, obtain regrowth.
The invention has the benefit that carrying out by after carrying out pre-processing to the seed selecting Varieties of Balsam Pear, disinfecting
Cultivating aseptic seedling, recycles the aseptic seedling that cultivation obtains to be cultivated in vitro, the cell tissue of balsam pear is placed in suitable induction and heals
Cultivate in the culture medium of wound and Bud Differentiation and obtain regrowth, the method can reduce seed costs, realizes factorial praluction, and then
Reduce the price of high-quality balsam pear, meet the market demand.
Specific embodiment
Below the embodiment it is clear that described is clearly and completely described to the technical scheme in the embodiment of the present invention
It is only a part of embodiment of the present invention, rather than whole embodiments.Based on embodiments of the invention, ordinary skill
The every other embodiment that personnel are obtained under the premise of not making creative work, broadly falls into protection scope of the present invention.
The embodiment of the present invention provides a kind of method cultivating balsam pear regrowth, can cultivate high-quality Varieties of Balsam Pear again
Raw seedling, comprises the following steps:
The seed selecting Varieties of Balsam Pear is pre-processed, disinfection after pretreatment, use the kind after disinfecting
Son carries out cultivating aseptic seedling, is cultivated in vitro using cultivating the aseptic seedling obtaining, the cell tissue of balsam pear is placed in and is suitable for luring
Lead in callus and the culture medium of Bud Differentiation and cultivate, obtain regrowth.
In said method, to the Seeds preprocess selecting Varieties of Balsam Pear it is: seed knife is all carved and removes, be immersed in no
In bacterium water, place dark treatment 24 hours in the refrigerator of 4 DEG C of temperature.
In said method, carry out after pretreatment disinfect for:
With the alcohol of 75% mass concentration, pretreated the surface of the seed is sterilized 30 seconds, repeatedly rinses 3 with sterilized water
Secondary, then the hypochlorite disinfectant 15 minutes with 0.2% mass concentration, repeatedly rinse 5 times with sterilized water.
In said method, carrying out cultivating aseptic seedling with the seed after disinfecting is:
Seed after pre-processing and sterilizing is inoculated on ms culture medium, is 25 DEG C in temperature, intensity of illumination is 2000lx,
The duration of day is to cultivate aseptic seedling under conditions of 16 hours.
In said method, cultivated in vitro using cultivating the aseptic seedling obtaining, the cell tissue of balsam pear is placed in suitable
Cultivate in the culture medium of callus induction and Bud Differentiation, obtain regrowth and include:
When the aseptic seedling cultivated grows to 8～12cm, carry out callus culture, the balsam pear material cutting is placed on culture medium
For in ms culture medium, concentration is the 6-ba of 1.5mg/l and concentration is 0.1mg/l naa hormone combinations, evoked callus and point
Change bud, obtain regrowth.
In said method, select the moonlight kind that Varieties of Balsam Pear is Beijing Agricultural College.
The culture medium prescription of the suitable balsam pear induction differentiation seedling of the embodiment of the present invention, before determining formula, first has to determine
The hormone being best suitable for breaking up is 6-ba and naa.Secondly allocate the concentration of ms+6-ba+naa, to meet balsam pear evoked callus
Condition with Bud Differentiation.Determine that final culture medium prescription is ms+1.5mg/l 6-ba+0.1mg/l naa through repeatedly debugging.
Below in conjunction with specific embodiment, the method for the present invention is described further.
Embodiment: the kind inducing differentiation and regeneration seedling with balsam pear is " moonlight " kind；
Operating process is: cultivates aseptic seedling → callus induction differentiation → Bud Differentiation；
(1) pretreatment of seed:
In in June, 2014, carry out the cultivation of balsam pear " moonlight " aseptic seedling in Beijing Agricultural College's Tissue Culture Center, first to kind
Son is pre-processed, and seed knife is all carved and removes, be immersed in sterilized water, places dark treatment 24h in 4 DEG C of refrigerators, due to
Balsam pear kind skin is really up to the mark, it will hinder embryonic development, and by kind of skin completely remove placement culture medium on be conducive to it to fully absorb training
Required nutrient in foster base, so carry out pretreatment to seed be necessary；
(2) seed disinfection method:
With the alcohol of 75% mass concentration, surface sterilization 30s, sterilized water rinses 3 times repeatedly, then uses 0.2% mass concentration
Hypochlorite disinfectant 15min, sterilized water rinses 5 times repeatedly；
(3) cultivate aseptic seedling:
By the peeling seed after sterilization, aseptically place in ms culture medium, be finally putting into temperature and be 25 DEG C, illumination
Intensity is 2000lx, and intensity of sunshine is trained aseptic seedling in the culturing room for 16h；
(4) callus induction and Bud Differentiation:
When the aseptic seedling of culture grows to 10cm, carry out callus culture, the balsam pear material cutting is placed on culture medium
In the naa hormone combinations of the 6-ba+0.1mg/l for ms+1.5mg/l, evoked callus and Bud Differentiation, obtain regrowth.Profit
Regrowth can effectively be induced with the culture medium of this special formulation.The method efficiently solves the kind of high-quality Varieties of Balsam Pear
Sub- cost, is conducive to high-quality balsam pear to reduce and sells price, suitable ordinary consumer is bought.
The method of the present invention passes through tissue culture technique, solves the too high Varieties of Balsam Pear of seed costs, because of seed costs
The problem that height is unfavorable for promoting.The method makes the cell tissue of balsam pear differentiate new plant again, can effectively avoid seed
Cost, and reach and under artificial sterile working and suitable environmental condition, carry out artificial culture and factorial praluction on a large scale,
Be conducive to preservation and the counter-seas on production cultivation of the germ plasm resource to Varieties of Balsam Pear.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope of present disclosure, the change or replacement that can readily occur in,
All should be included within the scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of claims
Enclose and be defined.
Claims (2)
1. a kind of method cultivating balsam pear regrowth is it is characterised in that comprise the following steps:
The seed of the Varieties of Balsam Pear selecting is pre-processed, pretreatment removes particularly as follows: all carving seed knife, is immersed in
In sterilized water, place dark treatment 24 hours in the refrigerator of 4 DEG C of temperature, disinfection after pretreatment, disinfect particularly as follows:
With the alcohol of 75% mass concentration, pretreated the surface of the seed is sterilized 30 seconds, is repeatedly rinsed 3 times with sterilized water, then use
The hypochlorite disinfectant of 0.2% mass concentration 15 minutes, rinses 5 times repeatedly with sterilized water；
Seed after pre-processing and sterilizing is inoculated on ms culture medium, is 25 DEG C in temperature, intensity of illumination is 2000lx, sunshine
Length is to cultivate aseptic seedling under conditions of 16 hours；
When the aseptic seedling cultivated grows to 8～12cm, it is that ms culture medium, concentration are that the balsam pear material cutting is placed on culture medium
The 6-ba of 1.5mg/l and concentration are in the naa hormone combinations of 0.1mg/l, evoked callus and Bud Differentiation, obtain regrowth.
2. the method cultivating balsam pear regrowth according to claim 1 is it is characterised in that the Varieties of Balsam Pear selecting is Beijing
The moonlight kind of agricultural college.
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| CN201510080859.2A CN104620989B (en) | 2015-02-13 | 2015-02-13 | Method for culturing bitter gourd regenerated seedlings |
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| CN201510080859.2A CN104620989B (en) | 2015-02-13 | 2015-02-13 | Method for culturing bitter gourd regenerated seedlings |
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| CN104620989B true CN104620989B (en) | 2017-01-18 |
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| CN107232059B (en) * | 2017-06-28 | 2019-09-13 | 山东博华高效生态农业科技有限公司 | A kind of tissue culture and rapid propagation method of dioecism momordica root |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104322370A (en) * | 2014-10-23 | 2015-02-04 | 广州市农业科学研究院 | Bitter gourd in vitro rapid propagation method |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104322370A (en) * | 2014-10-23 | 2015-02-04 | 广州市农业科学研究院 | Bitter gourd in vitro rapid propagation method |
Non-Patent Citations (4)
| Title |
|---|
| 不同激素组合对苦瓜离体快速繁殖的调控;王小荣等;《湖南师范大学自然科学学报》;20031231;第26卷(第4期);第76-78页 * |
| 苦瓜（Momordica charantia L.）组织培养基四倍体诱导研究;黄勇;《中国优秀硕士学位论文全文数据库 农业科技辑》;20071015(第4期);D048-15 * |
| 苦瓜子叶离体再生体系的建立;郑阳霞等;《北方园艺》;20111231(第15期);第163-165页 * |
| 苦瓜离体再生体系建立的研究;王国莉等;《安徽农业科学》;20081231;第36卷(第11期);第4408-4410页 * |
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