CN103749307B - The tissue culture propagation of short lobe China pink - Google Patents
The tissue culture propagation of short lobe China pink Download PDFInfo
- Publication number
- CN103749307B CN103749307B CN201410036700.6A CN201410036700A CN103749307B CN 103749307 B CN103749307 B CN 103749307B CN 201410036700 A CN201410036700 A CN 201410036700A CN 103749307 B CN103749307 B CN 103749307B
- Authority
- CN
- China
- Prior art keywords
- medium
- culture
- agar
- sucrose
- adds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses the tissue culture propagation of a kind of short lobe China pink, and have developed matching used substratum.This method, using stem with bud as explant, has that raw material quantity is many, advantage easily of drawing materials, and by tissue culture propagating, can shorten culture cycle and Fast-propagation goes out short lobe China pink high quality seedling.This method cultivates 30d bud breeding coefficient can reach 6.1 times; through expanding numerous, strong sprout and root culture; tissue cultured seedling rooting rate reaches more than 92.6%; after transplanting medium, surviving rate is more than 89.5%; efficiently solve the scale breeding problem of short lobe China pink; be conducive to producing seedling in batches, thus meet the need of market, there is higher using value.
Description
Technical field
The invention belongs to plant tissue culture breeding technology field, particularly relate to the tissue culture propagation of a kind of short lobe China pink.
Background technology
Short lobe China pink, deriving from the short lobe of Caryophyllaceae short lobe carnation (flower Brachystemma calycinum D.Don), is Caryophyllaceae Mono-species genus; Its root or all herbal medicine claim Root of Willowleaf Achyranthes (strengthening), knot grass (precious jade) etc., and taste is sweet, light, and property is put down; Have clearing heat and detoxicating, stimulate the circulation of the blood and cause the muscles and joints to relax, dispel rheumatism, effect of inducing diuresis to remove edema, synthetism myogenic, for diseases such as rheumatic arthralgia, wound, synthetism, nephritic edema, osteomyelitis, sore furuncle, tuberculous lymphadenitiss.Short lobe China pink is distributed in the provinces and regions such as China Guangxi, Sichuan, Yunnan, the Long Lin of main product in west, osmanthus, Tianlin County, reach the clouds, Napo County, Jingxi, Tiane, Nandan, the ground such as Donglan.
Short lobe China pink commonly uses the strong precious jade medicinal material of tradition simply.In the strong precious jade area in west, osmanthus, most resident commonly uses this medicine and pig cylinder bone is stewed together, is healthy and strong to the strong muscle of children, takes good care of health to the people of body void after being ill; Doctor among the people is with this medicine for main ingredient, and the disease such as treatment lumbar muscle strain, rheumatic arthritis, carpopedal spasm, after being ill weakness has special effect.In view of its definite effect, Guangxi Wei Jian pharmaceutcal corporation, Ltd is developed to exclusive herbal species " except stasis of blood restoring and treating injured soft tissues falls apart ", situation of selling well Hongkong and Macro and south east asia; Meanwhile, big strong medicine company also likes the custom of Baoshang according to the usage of Baoshang among the people and Guangdong,Hongkong and Macao resident, plans it and develops into and resemble the such soup stock of Herba Abri, Rhizome of Glabrous Greenbrier, Root of Beautiful Millettia.Along with continually developing of its series product, production-scale continuous expansion, raw materials requirement increases to about 5 tons from the hundreds of kilogram of 2007, make its resource day by day in short supply, the Long Lin of medicinal material purchase from Tianlin County, native country to periphery, the county such as to reach the clouds are again to neighbouring province Yunnan, Guizhou and other places, and existing wild resource has been difficult to the production-scale needs meeting expanding day.For strengthening the protection and developmental utilization to short lobe China pink, need widely popularize the cultivating and growing of short lobe China pink, realize its artificial culture, the primary problem solved is exactly breeding of seedling.The main mode of reproduction of current short lobe China pink is that cuttage and plant division are bred, but this modes of reproduction is difficult to realize amount reproduction seedling in a short time, and through too much for after plant division, plant vivotoxin increases, and the stalwartness affecting plant is grown up; And adopt biotechnology tissue culture technique, its reproduction speed can be accelerated and obtain a large amount of test-tube plantlet, the needs in seedling market can be met.
Summary of the invention
The technical problem to be solved in the present invention be to provide a kind of easy to operation, culture cycle is short, breed the tissue culture propagation of the high short lobe China pink of quick surviving rate.
For solving the problems of the technologies described above, the present invention by the following technical solutions: the tissue culture propagation of short lobe China pink, comprises the following steps:
(1) the drawing materials and sterilization of explant: get the stem with bud that short lobe China pink cuts into 2-3cm and carry out disinfection as explant;
(2) acquisition of in vitro cuttings: the explant disinfected is placed in inducing culture carry out adventitious bud inducing germinate obtain in vitro cuttings;
(3) test-tube plantlet breeding is cultivated: in vitro cuttings is placed in proliferated culture medium and carries out a large amount of indefinite bud of cultivation acquisition;
(4) the growth of plants is cultivated: the indefinite bud after breeding is placed in strong seedling culture base and carries out strong seedling culture and obtain healthy and strong plant;
(5) rooting of vitro seedling is cultivated: healthy and strong plant is placed in root media cultivation and obtains complete band offspring;
(6) test-tube plantlet acclimatization and transplants: screen to transplant after complete band offspring carries out hardening and cultivate in matrix, then transplant to land for growing field crops;
Inducing culture take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, Medium's PH Value is 5.8, and adds 1.0mgL
-16-benzyladenine (6-BA), 0.1mgL
-1naphthylacetic acid (NAA);
Proliferated culture medium take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.1-2.0mgL
-16-benzyladenine (6-BA), 0.01-1.0mgL
-1naphthylacetic acid (NAA), 0.1-0.5mgL
-1indolylacetic acid (IAA);
Strong seedling culture base take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.1-1.5mgL
-16-benzyladenine (6-BA), 0.1-2mgL
-1naphthylacetic acid (NAA);
Root media take 1/2MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.05-1.0mgL
-1indolebutyric acid (IBA), 0.5-2.0mgL
-1naphthylacetic acid (NAA).
Sterilization in step (1) is undertaken by following operation: first dripped by 2-3 in the beaker of liquid detergent instillation dress 50ml tap water, explant is put into beaker and stirs 2min gently, then clean explant surface smut with cotton, the slight running water 8-10min of tap water; Dislocation Bechtop, with 75% alcohol immersion 30S, sterilized water (distilled water after autoclaving) rinses one time, and then be 0.1% mercuric chloride sterilization 6-8min, aseptic water washing 3-5 time by the 50ml concentration that with the addition of 1-2 and drip tween 20, finally place the stainless steel plate disinfected, cut into the stem with bud that 1.0-1.5cm is long, obtain aseptic explant.
The culture condition of step (2) to (5) is: temperature is 23-25 DEG C, intensity of illumination 1500-2000lux, and light application time is 10-12h/d.
The incubation time of step (2) to (5) is respectively: 15-20d, 30d, 30d, 25d.
Acclimatization and transplants in step (6) is undertaken by following operation: the whole plant obtaining band root through step (5), treat that seedling grows to 5-6cm, when root grows to 2-4cm, the bottle seedling that growth selection is good, neat, healthy and strong, hardening is carried out in the indoor being 23-25 DEG C in room temperature, and in bottle, add a small amount of tap water flood substratum, hardening 5-7d, takes out test-tube plantlet with tweezers, cleans root substratum, be transplanted to growth 40-50d in matrix (being well advisable with loose ventilative, draining), then transplant to land for growing field crops.
Matrix is that perlite, sandstone and silt mix in the ratio of 1:2:1.
The substratum of short lobe China pink tissue culture, comprises inducing culture, proliferated culture medium, strong seedling culture base, root media,
Inducing culture take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, Medium's PH Value is 5.8, and adds 1.0mgL
-16-benzyladenine (6-BA), 0.1mgL
-1naphthylacetic acid (NAA);
Proliferated culture medium take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.1-2.0mgL
-16-benzyladenine (6-BA), 0.01-1.0mgL
-1naphthylacetic acid (NAA), 0.1-0.5mgL
-1indolylacetic acid (IAA);
Strong seedling culture base take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.1-1.5mgL
-16-benzyladenine (6-BA), 0.1-2mgL
-1naphthylacetic acid (NAA);
Root media take 1/2MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.05-1.0mgL
-1indolebutyric acid (IBA), 0.5-2.0mgL
-1naphthylacetic acid (NAA).
Breed Problems existing for current short lobe China pink, we have established the tissue culture propagation of a kind of short lobe China pink, and have developed matching used substratum.This method, using stem with bud as explant, has that raw material quantity is many, advantage easily of drawing materials, and passes through tissue culture propagating; culture cycle can be shortened and Fast-propagation goes out short lobe China pink high quality seedling; be conducive to large-scale production seedling, thus meet the need of market, there is higher using value.
Outstanding advantages of the present invention is:
<1> adopts the short lobe China pink of biotechnological means to carry out tissue culture propagating, by induction and the breeding of short lobe China pink indefinite bud, the proterties of original variety can be kept, can breed in a short time and obtain the consistent plant of a large amount of proterties, meet need of production, and for providing the short lobe China pink industrial seedling rearing of good character to provide reference.
<2> MS and 1/2MS solid medium used comprises macro-and microelements, the chemical agent lower costs such as the 6-benzyladenine added, naphthylacetic acid, indolylacetic acid and indolebutyric acid, concentration are suitable for, concrete effect is as follows: 6-BA and NAA in inducing culture, can sprout by evoking adventive bud; In proliferated culture medium, 6-BA, NAA and IAA can promote the propagation of bud; In strong seedling culture base, 6-BA and NAA can promote that seedling strengthens the propagation again with bud; In 1/2MS root media, IBA and NAA can promote seedling rooting, transplants the nutrient cup to dress matrix after hardening.
This method of <3> cultivates 30d bud breeding coefficient can reach 6.1 times; through expanding numerous, strong sprout and root culture; tissue cultured seedling rooting rate reaches more than 92.6%, and after transplanting medium, surviving rate is more than 89.5%, efficiently solves the scale breeding problem of short lobe China pink.
Embodiment
Embodiment 1
(1) the drawing materials and sterilization of explant: get the stem with bud that short lobe China pink cuts into 2-3cm and carry out disinfection as explant; First 2-3 is dripped in the beaker of liquid detergent instillation dress 50ml tap water, explant is put into beaker and stirs 2min gently, then clean explant surface smut with cotton, the slight running water 8-10min of tap water; Dislocation Bechtop, with 75% alcohol immersion 30S, sterilized water (distilled water after autoclaving) rinses one time, and then be 0.1% mercuric chloride sterilization 6-8min, aseptic water washing 3-5 time by the 50ml concentration that with the addition of 1-2 and drip tween 20, finally place the stainless steel plate disinfected, cut into the stem with bud that 1.0-1.5cm is long, obtain aseptic explant.
(2) acquisition of in vitro cuttings: the explant disinfected is placed in inducing culture and carries out adventitious bud inducing germination (temperature is 23-25 DEG C, intensity of illumination 1500-2000lux, and light application time is 10-12h/d) 15-20d, obtain in vitro cuttings;
(3) test-tube plantlet multiplication culture: in vitro cuttings is placed in proliferated culture medium and carries out cultivating (temperature is 23-25 DEG C, intensity of illumination 1500-2000lux, and light application time is 10-12h/d) 30d, obtain a large amount of indefinite bud, adventitious bud proliferation coefficient is 3.8;
(4) the growth of plants is cultivated: the indefinite bud after breeding is placed in strong seedling culture base and carries out strong seedling culture (temperature is 23-25 DEG C, intensity of illumination 1500-2000lux, light application time is 10-12h/d) 30d, obtain healthy and strong plant, indefinite bud secondary growth coefficient is 2.4;
(5) rooting of vitro seedling is cultivated: healthy and strong plant being placed in MS root media, to cultivate 25d(temperature be 23-25 DEG C, intensity of illumination 1500-2000lux, and light application time is 10-12h/d), obtain complete band offspring, rooting rate is 92.6%;
(6) test-tube plantlet acclimatization and transplants: the whole plant obtaining band root through step (5), treat that seedling grows to 5-6cm, when root grows to 2-4cm, the bottle seedling that growth selection is good, neat, healthy and strong, hardening is carried out in the indoor being 23-25 DEG C in room temperature, and in bottle, add a small amount of tap water flood substratum, hardening 5-7d, test-tube plantlet is taken out with tweezers, clean root substratum, be transplanted to that matrix (perlite, sandstone and silt mixs in the ratio of 1:2:1, are well advisable with loose ventilative, draining) is middle grows 40-50d, transplant to land for growing field crops, surviving rate is 92% again.
Wherein, the culture medium prescription of each stage use is as follows:
Inducing culture take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, Medium's PH Value is 5.8, and adds 1.0mgL
-16-benzyladenine (6-BA), 0.1mgL
-1naphthylacetic acid (NAA);
Proliferated culture medium take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.1mgL
-16-benzyladenine (6-BA), 0.05mgL
-1naphthylacetic acid (NAA), 0.1mgL
-1indolylacetic acid (IAA);
Strong seedling culture base take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.1mgL
-16-benzyladenine (6-BA), 0.1mgL
-1naphthylacetic acid (NAA);
Root media take 1/2MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.05mgL
-1indolebutyric acid (IBA), 0.5mgL
-1naphthylacetic acid (NAA).
Embodiment 2
Other are substantially with embodiment 1, and only following content is different:
In step (3), proliferated culture medium take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 1.0mgL
-16-benzyladenine (6-BA), 0.5mgL
-1naphthylacetic acid (NAA), 0.3mgL
-1indolylacetic acid (IAA); Adventitious bud proliferation coefficient is 6.1.
In step (4), strong seedling culture base take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.8mgL
-16-benzyladenine (6-BA), 1.0mgL
-1naphthylacetic acid (NAA), indefinite bud secondary growth coefficient is 2.9;
In step (5), root media take 1/2MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.1mgL
-1indolebutyric acid (IBA), 1.0mgL
-1naphthylacetic acid (NAA), rooting rate is 99.2%.
In step (6), surviving rate is 90%.
Embodiment 3
Other are substantially with embodiment 1, and only following content is different:
In step (3), proliferated culture medium take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 2.0mgL
-16-benzyladenine (6-BA), 1.0mgL
-1naphthylacetic acid (NAA), 0.5mgL
-1indolylacetic acid (IAA); Adventitious bud proliferation coefficient is 4.3.
In step (4), strong seedling culture base take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 1.5mgL
-16-benzyladenine (6-BA), 2.0mgL
-1naphthylacetic acid (NAA), indefinite bud secondary growth coefficient is 3.2;
In step (5), root media take 1/2MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 1.0mgL
-1indolebutyric acid (IBA), 2.0mgL
-1naphthylacetic acid (NAA), rooting rate is 94.7%.
In step (6), surviving rate is 88%.
Claims (4)
1. a tissue culture propagation for short lobe China pink, is characterized in that comprising the following steps:
(1) the drawing materials and sterilization of explant: get the stem with bud that short lobe China pink cuts into 2-3cm and carry out disinfection as explant;
(2) acquisition of in vitro cuttings: the explant disinfected is placed in inducing culture carry out adventitious bud inducing germinate obtain in vitro cuttings;
(3) test-tube plantlet multiplication culture: in vitro cuttings is placed in proliferated culture medium and carries out a large amount of indefinite bud of cultivation acquisition;
(4) the growth of plants is cultivated: the indefinite bud after breeding is placed in strong seedling culture base and carries out strong seedling culture and obtain healthy and strong plant;
(5) rooting of vitro seedling is cultivated: healthy and strong plant is placed in root media cultivation and obtains complete band offspring;
(6) test-tube plantlet acclimatization and transplants: screen to transplant after complete band offspring carries out hardening and cultivate in matrix, then transplant to land for growing field crops;
Described inducing culture take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, Medium's PH Value is 5.8, and adds 1.0mgL
-16-benzyladenine, 0.1mgL
-1naphthylacetic acid;
Described proliferated culture medium take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.1-2.0mgL
-16-benzyladenine, 0.01-1.0mgL
-1naphthylacetic acid, 0.1-0.5mgL
-1indolylacetic acid;
Described strong seedling culture base take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.1-1.5mgL
-16-benzyladenine, 0.1-2mgL
-1naphthylacetic acid;
Described root media take 1/2MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.05-1.0mgL
-1indolebutyric acid, 0.5-2.0mgL
-1naphthylacetic acid;
Sterilization in step (1) is undertaken by following operation: first dripped by 2-3 in the beaker of liquid detergent instillation dress 50ml tap water, explant is put into beaker and stirs 2min gently, again with cotton cleaning explant surface smut, the slight running water 8-10min of tap water; Dislocation Bechtop, with 75% alcohol immersion 30s, aseptic water washing one time, and then be 0.1% mercuric chloride sterilization 6-8min, aseptic water washing 3-5 time by the 50ml concentration that with the addition of 1-2 and drip tween 20, finally place the stainless steel plate disinfected, cut into the stem with bud that 1.0-1.5cm is long, obtain aseptic explant;
The culture condition of step (2) to (5) is: temperature is 23-25 DEG C, intensity of illumination 1500-2000lux, and light application time is 10-12h/d;
Acclimatization and transplants in step (6) is undertaken by following operation: the whole plant obtaining band root through step (5), treat that seedling grows to 5-6cm, when root grows to 2-4cm, the bottle seedling that growth selection is good, neat, healthy and strong, hardening is carried out in the indoor being 23-25 DEG C in room temperature, and in bottle, add a small amount of tap water flood substratum, hardening 5-7d, takes out test-tube plantlet with tweezers, cleans root substratum, be transplanted in matrix and grow 40-50d, then transplant to land for growing field crops.
2. the tissue culture propagation of short lobe China pink according to claim 1, is characterized in that the incubation time of step (2) to (5) is respectively: 15-20d, 30d, 30d, 25d.
3. the tissue culture propagation of short lobe China pink according to claim 2, is characterized in that: described matrix is that perlite, sandstone and silt mix in the ratio of 1:2:1.
4. the substratum of short lobe China pink tissue culture, comprises inducing culture, proliferated culture medium, strong seedling culture base, root media, it is characterized in that:
Described inducing culture take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, Medium's PH Value is 5.8, and adds 1.0mgL
-16-benzyladenine, 0.1mgL
-1naphthylacetic acid;
Described proliferated culture medium take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.1-2.0mgL
-16-benzyladenine, 0.01-1.0mgL
-1naphthylacetic acid, 0.1-0.5mgL
-1indolylacetic acid;
Described strong seedling culture base take MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.1-1.5mgL
-16-benzyladenine, 0.1-2mgL
-1naphthylacetic acid;
Described root media take 1/2MS as minimum medium, additional 4.5gL
-1agar, 30gL
-1sucrose, 1gL
-1gac, Medium's PH Value is 5.8, and adds 0.05-1.0mgL
-1indolebutyric acid, 0.5-2.0mgL
-1naphthylacetic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410036700.6A CN103749307B (en) | 2014-01-26 | 2014-01-26 | The tissue culture propagation of short lobe China pink |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410036700.6A CN103749307B (en) | 2014-01-26 | 2014-01-26 | The tissue culture propagation of short lobe China pink |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103749307A CN103749307A (en) | 2014-04-30 |
| CN103749307B true CN103749307B (en) | 2015-09-02 |
Family
ID=50516766
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410036700.6A Expired - Fee Related CN103749307B (en) | 2014-01-26 | 2014-01-26 | The tissue culture propagation of short lobe China pink |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103749307B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258294A (en) * | 2015-06-24 | 2017-01-04 | 黄保成 | A kind of implantation methods of Brachystemma calycina D. Don. |
| CN105265320B (en) * | 2015-11-18 | 2017-11-07 | 广西壮族自治区药用植物园 | A kind of tissue culture propagation of aristolochia mollissima |
| CN107155604A (en) * | 2017-06-16 | 2017-09-15 | 内蒙古蒙草生态环境（集团）股份有限公司 | The cultivation management method of " girl in red carnation " |
| CN116711639B (en) * | 2023-06-26 | 2024-06-11 | 安徽农业大学 | Culture medium combination for preventing tissue culture browning of stevia rebaudiana and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103262796A (en) * | 2013-05-27 | 2013-08-28 | 巴中市光雾山植物研究所 | Method for rapidly breeding potted carnation through tissue culture |
-
2014
- 2014-01-26 CN CN201410036700.6A patent/CN103749307B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103262796A (en) * | 2013-05-27 | 2013-08-28 | 巴中市光雾山植物研究所 | Method for rapidly breeding potted carnation through tissue culture |
Non-Patent Citations (2)
| Title |
|---|
| 兴安石竹茎段离体繁殖的研究;李桂君等;《黑龙江生态工程职业学院学报》;20100331;第23卷(第2期);第18-19页 * |
| 高山石竹组织培养及植株再生;吴捷等;《中国林副特产》;20091231(第6期);第39页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103749307A (en) | 2014-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105145352B (en) | A kind of bletilla seed height of seedling imitates group culturation rapid propagating technology | |
| CN104106468B (en) | The quick breeding method for tissue culture of a kind of radix fici simplicissimae | |
| CN101564008A (en) | Hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings | |
| CN103749307B (en) | The tissue culture propagation of short lobe China pink | |
| CN101897297B (en) | Two-step tissue culture quick propagation method for hemerocallis | |
| CN105265320B (en) | A kind of tissue culture propagation of aristolochia mollissima | |
| CN105230484A (en) | Rapid breeding method of rhizoma polygonati | |
| CN109220790A (en) | A kind of in vitro outer breeding method of red fruit ginseng | |
| CN106613960B (en) | A kind of Helen's pocket orchid callus regeneration system rapid propagation method | |
| CN104137779A (en) | Method for regenerating sapium japonicum plant by inducing sapium japonicum stem rapidly | |
| CN105409771A (en) | Rapid propagation method for Gymnadenia conopsea (L.) R. Br. | |
| CN104719168B (en) | The method for cultivating bletilla striata seedling using interval submergence bioreactor | |
| CN106818468A (en) | A kind of shellflower seed asepsis sprouting and rapid propagation method | |
| CN109479638A (en) | A kind of the muskmelon seedling fostering method and muskmelon seedling of high disease resistance ability | |
| CN105830918A (en) | Method for improving survival rate of transplanting of Huperzia serrata spores | |
| CN103477976B (en) | A kind of Herba Dendrobii stem section tissue culture method | |
| CN104813931A (en) | Tissue culture and rapid propagation method for Dendrobium officinale | |
| CN102232359B (en) | In-vitro rapid propagation method of double-petal Jasminum sambac | |
| CN104855289B (en) | Method for culturing and producing micro-bulbodium of crocus sativus L. through superficial layer | |
| CN104604680B (en) | Can promote that bletilla striata seeds sprouts the culture medium with growth of seedling and formula thereof and preparation method | |
| CN105918132A (en) | Rapid breeding method of clerodendrum trichotomum thunb | |
| CN105532472A (en) | Direct induction seedling development method for hybrid cymbidium tortisepalum rhizomes | |
| CN107873524A (en) | A kind of serpentgrass stem segment tissue culture fast breeding method | |
| CN105815215A (en) | Tissue culture propagation method for lysimachia foenum-graecum | |
| CN107535354A (en) | A kind of scientific and effective river monkshood propagation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150902 Termination date: 20160126 |
|
| EXPY | Termination of patent right or utility model |