CN109479638A - A kind of the muskmelon seedling fostering method and muskmelon seedling of high disease resistance ability - Google Patents
A kind of the muskmelon seedling fostering method and muskmelon seedling of high disease resistance ability Download PDFInfo
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- CN109479638A CN109479638A CN201811560303.3A CN201811560303A CN109479638A CN 109479638 A CN109479638 A CN 109479638A CN 201811560303 A CN201811560303 A CN 201811560303A CN 109479638 A CN109479638 A CN 109479638A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses the muskmelon seedling fostering method and muskmelon seedling of a kind of high disease resistance ability, include the following steps: that (1) handles muskmelon seeds using alternating magnetic field；(2) seedling medium is sterilized, is then mixed with containing beneficial microorganism mixed solution, for use；(3) it sows；(4) cultivation and the observation of emergence rate before emergence；(5) cultivate after emerging: quantity to be emerged reaches certain amount, then cultivated in heliogreenhouse, and daytime and night carry out selecting different breeding conditions respectively, when a certain amount of muskmelon seedling grows at least 2 true leaves, solution containing liacinin is sprayed on blade face, and carries out pouring root；When growing at least 4 true leaves to muskmelon seedling wait cultivate, so that it may be transplanted, that is, cultivate the muskmelon seedling of high disease resistance ability；And the muskmelon seedling cultivated by the above method；The present invention can effectively improve the germination percentage of muskmelon seeds, and significantly improve the ability of muskmelon seedling disease resistance.
Description
Technical field
The invention belongs to muskmelon Cultivating techniques fields, and in particular to a kind of muskmelon seedling fostering method of high disease resistance ability
And muskmelon seedling.
Background technique
Muskmelon (Cucumis melo L.) is also known as muskmelon, fruit melon, sweet melon, ripe melon, is that the annual tendril draft of Curcurbitaceae is planted
Object；Muskmelon is that China is utilized as the melon of fruit earliest, throughout our country plantation extensively, common muskmelon, "Hami" melon, honey dew melon etc.
It is belonging respectively to the different lines of muskmelon.Muskmelon contain a large amount of carbohydrate and citric acid, carrotene, B family vitamin and
Vitamin C etc., is important one of fruit.However in the planting process of muskmelon, vulnerable to infecting for multiple diseases, to lead
Cause Muskmelon Planting the underproduction, it is serious when result even in total crop failure.
And in the planting process of muskmelon, seedling stage grow up the case where be the key that whether there is in the later period stronger disease resistance, because
For the cultivation of muskmelon nursery, there are many evolutionary approach, such as Chinese invention patent in this currently available technology
CN106665055A, it discloses a kind of anti obstacle to continuous cropping netted melon seeding cultivating method, the technology specifically used is in muskmelon
Seedling Stage carries out resistance seedling fostering using the method for vaccinization (pathogenic bacteria), and the technical method is for technical operation personnel's
It is more demanding, and spend the time longer, it is unfavorable for promoting the use of；
For another example Chinese invention patent CN105230310A, it discloses a kind of production methods of high-quality muskmelon seedling, specifically
The technology used is graft technology, and using pumpkin seedlings as the engrafting and cultivating of stock progress muskmelon seedling, this is equally to operator
Member's is more demanding, and a possibility that change there are muskmelon mouthfeel, is unfavorable for large-scale application；
For another example Chinese invention patent CN106665047A, it discloses a kind of cultural methods of Melon, wherein using
Nutrient solution in containing honeysuckle, achyranthes aspera, kuh-seng, phoenix tree leaf, tripterygium wilfordii, cordate houttuynia, Lindley Butterflybush Herb extract, not only sweet tea
The growth of melon provides nutriment, while also there is sterilization and disinsection to reduce disease；It falls ill simultaneously added with having to kill to have
Chlorobromide isocyanuric acid, Bravo, the mandipropamid of the effect of worm；Method and technology for breeding phase is using conventional work
Object Cultivating techniques, while cultivating that cost is too high, and without improving the specific aim of muskmelon breeding.
Summary of the invention
The technical problem to be solved by the present invention is to overcome deficiency in the prior art, a kind of improved high disease resistance is provided
The muskmelon seedling fostering method of ability, can effectively improve the germination percentage of muskmelon seeds, and it is disease-resistant to significantly improve muskmelon seedling
Harmful ability.
The present invention goes back while providing a kind of muskmelon seedling of high disease resistance that above-mentioned breeding method is cultivated.
In order to solve the above technical problems, a kind of technical solution that the present invention takes is as follows:
A kind of muskmelon seedling fostering method of high disease resistance ability, the breeding method include the following steps:
(1) seed treatment: muskmelon seeds are immersed in sodium chloride solution, then will be immersed with the sodium chloride of muskmelon seeds
Solution is placed in alternating magnetic field, is taken out, and is cleaned, sterilization, then will treated muskmelon seeds are stored in temperature is 20-25 DEG C,
Humidity is in the environment of 20-30%, in case sowing；
Wherein, the alternative frequency of the alternating magnetic field is 8-12Hz, and the intensity of central magnetic field is 1000-2000Gs；
(2) seedling medium is handled: seedling medium is sterilized, is then mixed with containing beneficial microorganism mixed solution, to
With；
Wherein, the beneficial microbe is to conciliate starch gemma selected from bacillus subtilis, saccharomycete, bacillus licheniformis
At least two combination in bacillus；
(3) it sows: the muskmelon seeds that step (1) is handled well being planted in the seedling medium that step (2) is handled well；
(4) seedling medium that in step (3) kinds are implanted with muskmelon seeds cultivation and the observation of emergence rate before emergence: is placed in temperature
Degree stands and cultivates to cover sunshade net above in 25-35 DEG C of environment；The humidity of observation seedling medium daily, is replenished in time water
Point, observe emergence rate；
(5) it is cultivated after emerging: when the quantity wait emerge reaches 90% or more, removing covering sunshade net, kind is implanted with muskmelon
The seedling medium of seed is cultivated in heliogreenhouse, is periodically watered；
Wherein, the breeding condition on daytime is as follows: temperature is 25-30 DEG C, humidity 60-70%；
The condition of culture at night is as follows: temperature is 20-25 DEG C, humidity 50-60%；In night culture, LED light is utilized
Carry out light filling；The arrangement of night lights irradiation are as follows: red-light LED light-illuminating 0.5-1.5h, blue-ray LED light-illuminating 0.5-
1.5h, without light-illuminating 0.5-1.5h, so alternately, night complete altogether 4 times circulation；
When 90% muskmelon seedling grows at least 2 true leaves, the solution containing liacinin is sprayed on blade face,
And carry out pouring root；When growing at least 4 true leaves to muskmelon seedling wait cultivate, so that it may be transplanted, that is, cultivate the highly resistance
The muskmelon seedling of disease ability.
In the present invention, step (1) and step (2) are in no particular order.
In terms of more according to the present invention specific and preferred, in step (1), by the leaching at being 24-26 DEG C in temperature
Bubble has the sodium chloride solution of muskmelon seeds to be placed in alternating magnetic field, time of repose 20-30min.
It is described clean clear using distilled water progress in step (1) in terms of more according to the present invention specific and preferred
It washes.
In terms of more according to the present invention specific and preferred, in step (1), before carrying out the sterilization operation, first dry in the air
It is dry, and the sterilization is sterilized using ultraviolet light irradiation.
Some preferred aspects according to the present invention, in step (2), the sterilizing carries out at being 200-300 DEG C in temperature, goes out
The bacterium time is 20-30s.
Some preferred aspects according to the present invention, in step (2), the beneficial microbe is by bacillus subtilis, yeast
Bacterium, bacillus licheniformis and bacillus amyloliquefaciens are constituted.
Some preferred aspects according to the present invention, in step (2), in the mixed solution, the bacillus subtilis
Content is 5 × 107CFU/mL-8×107CFU/mL。
Some preferred aspects according to the present invention, in step (2), in the mixed solution, the content of the saccharomycete is 2
×106CFU/mL-8×106CFU/mL。
Some preferred aspects according to the present invention, in step (2), in the mixed solution, the bacillus licheniformis
Content is 2 × 106CFU/mL-1×107CFU/mL。
Some preferred aspects according to the present invention, in step (2), in the mixed solution, the bacillus amyloliquefaciens
Content be 5 × 106CFU/mL-2×107CFU/mL。
Some specific aspects according to the present invention, in step (3), when being sowed, according to 1,1 cave muskmelon seeds
Mode is sowed, and is after planting poured with water.
Some preferred aspects according to the present invention, in step (5), the arrangement of night lights irradiation are as follows: red LED lamp illumination
Penetrate 0.9-1.1h, blue-ray LED light-illuminating 0.9-1.1h, without light-illuminating 0.9-1.1h, so alternately, night completes altogether
4 circulations.A specific aspect according to the present invention, in step (5), the arrangement of night lights irradiation are as follows: red LED lamp illumination
Penetrate 1h, blue-ray LED light-illuminating 1h, without light-illuminating 1h, so alternately, night completes 4 circulations altogether.
Some preferred aspects according to the present invention, will when 90% muskmelon seedling grows the 2nd true leaf in step (5)
Solution containing liacinin sprays on blade face, and carries out pouring root；When growing the 4th true leaf to muskmelon seedling wait cultivate,
It can be carried out transplanting.
Some preferred aspects according to the present invention, in step (5), the content of the liacinin is 3 × 106CFU/
mL-9×106CFU/mL。
A kind of another technical solution provided by the invention: muskmelon seedling fostering method of high disease resistance ability described above
The muskmelon seedling of cultivation.
Due to the use of above technical scheme, the invention has the following advantages over the prior art:
The present invention selects specific alternating magnetic field processing for muskmelon seeds, before seedling medium processing, emergence
Cultivated after cultivation and the observation of emergence rate, emergence and etc., it carries out selecting different trainings respectively in particular for daytime and night
Condition is educated, the gene expression of muskmelon seedling is changed, and then imparts the excellent disease resistance of muskmelon seedling, while muskmelon seeds
Germination percentage be also significantly improved, and breeding method of the invention is easy to operate, and the requirement to technical staff is lower, into
And it is suitable for large-scale promotion.
Specific embodiment
Above scheme is described further below in conjunction with specific embodiment；It should be understood that these embodiments are for illustrating
The basic principles, principal features and advantages of the present invention, and the present invention is not by the scope limitation of following embodiment；It is used in embodiment
Implementation condition further adjustment can be done according to specific requirement, the implementation condition being not specified is usually the item in routine experiment
Part.In following, unless otherwise specified, all raw materials are both from commercially available.
Embodiment 1:
(1) full grains, unabroken muskmelon seeds 400 are chosen, wherein 200 carry out according to breeding method of the present invention
Nursery, in addition 200 seed conventionally nursery, as control；
(2) the specific seedling raising process by breeding method of the present invention processing is as follows:
A, at 25 DEG C, by muskmelon seeds be immersed in saturation NaCl solution in, then by immersed with muskmelon seeds
Saturation NaCl solution be placed in that alternative frequency is 10Hz, central magnetic field is in the magnetic field of 1000Gs, stand 20min；
B, it after taking-up, is cleaned, is dried, with ultraviolet light irradiation 30s with distilled water；
C, then will treated muskmelon seeds are stored in temperature be 20 DEG C, humidity is in 20% environment, in case sowing is used；
D, the business seedling medium of purchase is heated into 30s at 200 DEG C using baking oven；
E, it in the seedling medium after high-temperature process, is added and contains beneficial microorganism mixed solution, wherein including withered grass
Bacillus (5 × 107CFU/mL), saccharomycete (2 × 106CFU/mL), bacillus licheniformis (2 × 106CFU/mL), starch is solved
Bacillus (5 × 106CFU/mL), uniformly mixing, is then laid down into seedlings nursing plate；
F, by the muskmelon seeds handled well according in 1, the 1 cave seed seedling medium that is seeded in that treated, sowing
Afterwards, it is poured with water；
G, seedlings nursing plate is placed in the environment that temperature is 25 DEG C, covers sunshade net above, stood and cultivate；
H, moisture is replenished in time in the humidity for observing seedling medium daily, observes emergence rate；
I, when the quantity wait emerge reaches 90% or more, covering sunshade net is removed, seedling disk is cultivated in heliogreenhouse,
Periodically watering；
J, the breeding condition on daytime is as follows: temperature is 25 DEG C, humidity 60%；The condition of culture at night is as follows: temperature is
20 DEG C, humidity 50%；In night culture, light filling is carried out using LED light；The arrangement of night lights irradiation is red LED lamp
Light irradiates 1h, blue-ray LED light-illuminating 1h, without light-illuminating (i.e. dark) 1h, and so alternately, night completes 4 times altogether and follows
Ring；
K, when 90% muskmelon seedling grows the 2nd true leaf, liacinin solution (3 × 10 will be contained6CFU/mL)
It sprays on blade face, and carries out pouring root；
L, it when growing the 4th true leaf to muskmelon seedling wait cultivate, is transplanted, that is, cultivates the high disease resistance ability
Muskmelon seedling.
(3) as the conventional seedbed system of control, detailed process is as follows:
A, a certain amount of water is added in the business culture medium for seedling of the same batch of purchase, be then laid down into seedlings nursing plate；
B, the muskmelon seeds handled well are sowed according to 1,1 cave seed, after planting, is poured with water；
C, seedlings nursing plate is placed in the environment that temperature is 25 DEG C, covers sunshade net above, stood and cultivate；
D, moisture is replenished in time in the humidity for observing seedling medium daily, observes emergence rate；
E, when the quantity wait emerge reaches 90% or more, covering sunshade net is removed, seedling disk is cultivated in heliogreenhouse,
Periodically watering, illumination are that natural daylight irradiates, the diurnal temperature and humidity without night light filling, not in controllable greenhouse；
F, it when growing the 5th true leaf to seedling wait cultivate, is transplanted.
(4) by the processing group and control group progress powdery mildew pathogenic bacteria induction experiment after transplanting, disease plant number is observed.
As a result as follows:
The result shows that effect of the invention is better than control group, not only emergence rate is improved, and significantly improves muskmelon
The mildew-resistance ability of plant.
Embodiment 2:
(1) full grains, unabroken muskmelon seeds 400 are chosen, wherein 200 carry out according to breeding method of the present invention
Nursery, in addition 200 seed conventionally nursery, as control；
(2) the specific seedling raising process by breeding method of the present invention processing is as follows:
A, at 25 DEG C, by muskmelon seeds be immersed in saturation NaCl solution in, then by immersed with muskmelon seeds
Saturation NaCl solution be placed in that alternative frequency is 10Hz, central magnetic field is in the magnetic field of 1500Gs, stand 25min；
B, it after taking-up, is cleaned, is dried, with ultraviolet light irradiation 45s with distilled water；
C, then will treated muskmelon seeds are stored in temperature be 20 DEG C, humidity is in 30% environment, in case sowing is used；
D, the business seedling medium of purchase is heated into 20s at 300 DEG C using baking oven；
E, it in the seedling medium after high-temperature process, is added and contains beneficial microorganism mixed solution, wherein including withered grass
Bacillus (6 × 107CFU/mL), saccharomycete (4 × 106CFU/mL), bacillus licheniformis (6 × 106CFU/mL), starch is solved
Bacillus (1 × 106CFU/mL), uniformly mixing, is then laid down into seedlings nursing plate；
F, by the muskmelon seeds handled well according in 1, the 1 cave seed seedling medium that is seeded in that treated, sowing
Afterwards, it is poured with water；
G, seedlings nursing plate is placed in the environment that temperature is 27 DEG C, covers sunshade net above, stood and cultivate；
H, moisture is replenished in time in the humidity for observing seedling medium daily, observes emergence rate；
I, when the quantity wait emerge reaches 90% or more, covering sunshade net is removed, seedling disk is cultivated in heliogreenhouse,
Periodically watering；
J, the breeding condition on daytime is as follows: temperature is 25 DEG C, humidity 70%；The condition of culture at night is as follows: temperature is
20 DEG C, humidity 60%；In night culture, light filling is carried out using LED light；The arrangement of night lights irradiation is red LED lamp
Light irradiates 1h, blue-ray LED light-illuminating 1h, without light-illuminating (i.e. dark) 1h, and so alternately, night completes 4 times altogether and follows
Ring；
K, when 90% muskmelon seedling grows the 2nd true leaf, liacinin solution (6 × 10 will be contained6CFU/mL)
It sprays on blade face, and carries out pouring root；
L, it when growing the 4th true leaf to muskmelon seedling wait cultivate, is transplanted, that is, cultivates the high disease resistance ability
Muskmelon seedling.
(3) as the conventional seedbed system of control, detailed process is as follows:
A, a certain amount of water is added in the business culture medium for seedling of the same batch of purchase, be then laid down into seedlings nursing plate；
B, the muskmelon seeds handled well are sowed according to 1,1 cave seed, after planting, is poured with water；
C, seedlings nursing plate is placed in the environment that temperature is 25 DEG C, covers sunshade net above, stood and cultivate；
D, moisture is replenished in time in the humidity for observing seedling medium daily, observes emergence rate；
E, when the quantity wait emerge reaches 90% or more, covering sunshade net is removed, seedling disk is cultivated in heliogreenhouse,
Periodically watering, illumination are that natural daylight irradiates, the diurnal temperature and humidity without night light filling, not in controllable greenhouse；
F, it when growing the 5th true leaf to seedling wait cultivate, is transplanted.
(4) by the processing group and control group progress downy mildew pathogenic bacteria induction experiment after transplanting, disease plant number is observed.
As a result as follows:
The result shows that effect of the invention is better than control group, not only emergence rate is improved, and significantly improves muskmelon
The downy mildew resistance ability of plant.
Embodiment 3:
(1) full grains, unabroken muskmelon seeds 400 are chosen, wherein 200 carry out according to breeding method of the present invention
Nursery, in addition 200 seed conventionally nursery, as control；
(2) the specific seedling raising process by breeding method of the present invention processing is as follows:
A, at 25 DEG C, by muskmelon seeds be immersed in saturation NaCl solution in, then by immersed with muskmelon seeds
Saturation NaCl solution be placed in that alternative frequency is 10Hz, central magnetic field is in the magnetic field of 2000Gs, stand 30min；
B, it after taking-up, is cleaned, is dried, with ultraviolet light irradiation 60s with distilled water；
C, then will treated muskmelon seeds are stored in temperature be 25 DEG C, humidity is in 30% environment, in case sowing is used；
D, the business seedling medium of purchase is heated into 30s at 300 DEG C using baking oven；
E, it in the seedling medium after high-temperature process, is added and contains beneficial microorganism mixed solution, wherein including withered grass
Bacillus (8 × 107CFU/mL), saccharomycete (8 × 106CFU/mL), bacillus licheniformis (1 × 107CFU/mL), starch is solved
Bacillus (2 × 107CFU/mL), uniformly mixing, is then laid down into seedlings nursing plate；
F, by the muskmelon seeds handled well according in 1, the 1 cave seed seedling medium that is seeded in that treated, sowing
Afterwards, it is poured with water；
G, seedlings nursing plate is placed in the environment that temperature is 30 DEG C, covers sunshade net above, stood and cultivate；
H, moisture is replenished in time in the humidity for observing seedling medium daily, observes emergence rate；
I, when the quantity wait emerge reaches 90% or more, covering sunshade net is removed, seedling disk is cultivated in heliogreenhouse,
Periodically watering；
J, the breeding condition on daytime is as follows: temperature is 30 DEG C, humidity 70%；The condition of culture at night is as follows: temperature is
25 DEG C, humidity 60%；In night culture, light filling is carried out using LED light；The arrangement of night lights irradiation is red LED lamp
Light irradiates 1h, blue-ray LED light-illuminating 1h, without light-illuminating (i.e. dark) 1h, and so alternately, night completes 4 times altogether and follows
Ring；
K, when 90% muskmelon seedling grows the 2nd true leaf, liacinin solution (9 × 10 will be contained6CFU/mL)
It sprays on blade face, and carries out pouring root；
L, it when growing the 4th true leaf to muskmelon seedling wait cultivate, is transplanted, that is, cultivates the high disease resistance ability
Muskmelon seedling.
(3) as the conventional seedbed system of control, detailed process is as follows:
A, a certain amount of water is added in the business culture medium for seedling of the same batch of purchase, be then laid down into seedlings nursing plate；
B, the muskmelon seeds handled well are sowed according to 1,1 cave seed, after planting, is poured with water；
C, seedlings nursing plate is placed in the environment that temperature is 25 DEG C, covers sunshade net above, stood and cultivate；
D, moisture is replenished in time in the humidity for observing seedling medium daily, observes emergence rate；
E, when the quantity wait emerge reaches 90% or more, covering sunshade net is removed, seedling disk is cultivated in heliogreenhouse,
Periodically watering, illumination are that natural daylight irradiates, the diurnal temperature and humidity without night light filling, not in controllable greenhouse；
F, it when growing the 5th true leaf to seedling wait cultivate, is transplanted.
(4) by the processing group and control group progress anthracnose pathogenic bacteria induction experiment after transplanting, disease plant number is observed.
As a result as follows:
The result shows that effect of the invention is better than control group, not only emergence rate is improved, and significantly improves muskmelon
The anti-anthracnose ability of plant.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art
Scholar cans understand the content of the present invention and implement it accordingly, and it is not intended to limit the scope of the present invention, it is all according to the present invention
Equivalent change or modification made by Spirit Essence, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of muskmelon seedling fostering method of high disease resistance ability, which is characterized in that the breeding method includes the following steps:
(1) seed treatment: muskmelon seeds are immersed in sodium chloride solution, then will be immersed with the sodium chloride solution of muskmelon seeds
It is placed in alternating magnetic field, takes out, clean, sterilization, then muskmelon seeds are stored in temperature as 20-25 DEG C, humidity by treated
For in the environment of 20-30%, in case sowing；
Wherein, the alternative frequency of the alternating magnetic field is 8-12Hz, and the intensity of central magnetic field is 1000-2000Gs；
(2) seedling medium is handled: seedling medium being sterilized, is then mixed with containing beneficial microorganism mixed solution, for use；
Wherein, the beneficial microbe is selected from bacillus subtilis, saccharomycete, bacillus licheniformis and bacillus amyloliquefaciens
In at least two combination；
(3) it sows: the muskmelon seeds that step (1) is handled well being planted in the seedling medium that step (2) is handled well；
(4) cultivation and the observation of emergence rate before emergence: the seedling medium that in step (3) kinds are implanted with muskmelon seeds, which is placed in temperature, is
In 25-35 DEG C of environment, sunshade net is covered above, is stood and is cultivated；The humidity of observation seedling medium daily, is replenished in time moisture,
Observe emergence rate；
(5) it is cultivated after emerging: when the quantity wait emerge reaches 90% or more, removing covering sunshade net, kind is implanted with muskmelon seeds
Seedling medium is cultivated in heliogreenhouse, is periodically watered；
Wherein, the breeding condition on daytime is as follows: temperature is 25-30 DEG C, humidity 60-70%；
The condition of culture at night is as follows: temperature is 20-25 DEG C, humidity 50-60%；In night culture, carried out using LED light
Light filling；The arrangement of night lights irradiation are as follows: red-light LED light-illuminating 0.5-1.5h, blue-ray LED light-illuminating 0.5-1.5h, nothing
Light-illuminating 0.5-1.5h, so alternately, night complete 4 circulations altogether；
When 90% muskmelon seedling grows at least 2 true leaves, the solution containing liacinin is sprayed on blade face, is gone forward side by side
Row pouring root；When growing at least 4 true leaves to muskmelon seedling wait cultivate, so that it may be transplanted, that is, cultivate the high disease resistance
The muskmelon seedling of ability.
2. the muskmelon seedling fostering method of high disease resistance ability according to claim 1, which is characterized in that in step (1),
The sodium chloride solution immersed with muskmelon seeds is placed in alternating magnetic field at being 24-26 DEG C in temperature, time of repose 20-
30min。
3. the muskmelon seedling fostering method of high disease resistance ability according to claim 1, which is characterized in that in step (1),
The clean use distilled water is cleaned；And/or in step (1), before carrying out the sterilization operation, first dry, and described
Sterilization is sterilized using ultraviolet light irradiation.
4. the muskmelon seedling fostering method of high disease resistance ability according to claim 1, which is characterized in that in step (2),
The sterilizing carries out at being 200-300 DEG C in temperature, sterilization time 20-30s.
5. the muskmelon seedling fostering method of high disease resistance ability according to claim 1, which is characterized in that in step (2),
The beneficial microbe is made of bacillus subtilis, saccharomycete, bacillus licheniformis and bacillus amyloliquefaciens.
6. the muskmelon seedling fostering method of high disease resistance ability according to claim 5, which is characterized in that in step (2),
In the mixed solution, the content of the bacillus subtilis is 5 × 107CFU/mL - 8×107CFU/mL, the saccharomycete
Content be 2 × 106CFU/mL - 8×106CFU/mL, the content of the bacillus licheniformis are 2 × 106CFU/mL - 1×
107CFU/mL, the content of the bacillus amyloliquefaciens are 5 × 106CFU/mL - 2×107CFU/mL。
7. the muskmelon seedling fostering method of high disease resistance ability according to claim 1, which is characterized in that in step (3),
When being sowed, is sowed in the way of the muskmelon seeds of 1,1 cave, after planting poured with water.
8. the muskmelon seedling fostering method of high disease resistance ability according to claim 1, which is characterized in that in step (5),
The arrangement of night lights irradiation are as follows: red-light LED light-illuminating 0.9-1.1h, blue-ray LED light-illuminating 0.9-1.1h, shone without light
0.9-1.1h is penetrated, so alternately, night completes 4 circulations altogether.
9. the muskmelon seedling fostering method of high disease resistance ability according to claim 1, which is characterized in that in step (5),
When 90% muskmelon seedling grows the 2nd true leaf, the solution containing liacinin is sprayed on blade face, and carry out pouring root；
When growing the 4th true leaf to muskmelon seedling wait cultivate, so that it may be transplanted；And/or
In step (5), the content of the liacinin is 3 × 106CFU/mL - 9×106CFU/mL。
10. the muskmelon seedling fostering method of high disease resistance ability described in a kind of any one of claim 1-9 claim is trained
The muskmelon seedling educated.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713206A (en) * | 2020-06-19 | 2020-09-29 | 四川省农业科学院植物保护研究所 | Method for increasing yield of bitter gourds by using biocontrol strain bacillus amyloliquefaciens Bam22 |
| CN114223472A (en) * | 2021-11-16 | 2022-03-25 | 广东省农业科学院动物科学研究所 | Culture method and application of high-yield cold-resistant stylosanthes guianensis |
| CN117581721A (en) * | 2024-01-19 | 2024-02-23 | 三亚市国家耐盐碱水稻技术创新中心 | Method for improving salt tolerance of rice in seedling stage by using triptfordine |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101693641A (en) * | 2009-10-22 | 2010-04-14 | 南京信息工程大学 | Compound bio-fertilizer containing ardealite and application thereof |
| CN101891511A (en) * | 2010-06-15 | 2010-11-24 | 李学麒 | Organic substrate suitable for planting vegetables, melons and fruits and preparation method thereof |
| CN103503683A (en) * | 2013-10-09 | 2014-01-15 | 薛军 | Method for cultivating seedlings of purebred lycium ruthenicum with high survival rate |
| CN104396511A (en) * | 2014-11-17 | 2015-03-11 | 成都红柿子科技有限公司 | Tomato seedling breeding technology |
| CN105409381A (en) * | 2015-11-26 | 2016-03-23 | 全椒县桃花湾果蔬种植专业合作社 | Caraway seed processing method |
| CN105409524A (en) * | 2015-11-26 | 2016-03-23 | 全椒县桃花湾果蔬种植专业合作社 | Method for planting high-quality Coriandrum sativum |
| CN105594448A (en) * | 2016-03-04 | 2016-05-25 | 夏志刚 | Artificial seedling rearing method of cucumber seeds |
| CN106879442A (en) * | 2017-03-03 | 2017-06-23 | 明光现代农业科技合作推广服务中心 | A kind of utilization LED light source promotes mung bean shoot vegetable quickly to turn green method |
| CN206895295U (en) * | 2017-03-31 | 2018-01-19 | 山东农业大学 | A kind of muskmelon seedling raising supplementary lighting device for suppressing powdery mildew and promoting strong sprout |
| CN107637429A (en) * | 2017-10-09 | 2018-01-30 | 广州市汇吉科技企业孵化器有限公司 | A kind of implantation methods of Silicon-rich muskmelon |
| CN107995843A (en) * | 2017-06-01 | 2018-05-04 | 苏州佳亿达电器有限公司 | LED light compensating apparatus for green plant |
| CN108738820A (en) * | 2018-06-20 | 2018-11-06 | 内蒙古蒙草生态环境（集团）股份有限公司 | A kind of interior lawn light compensation method and indoor lawn stage lawn formation method |
-
2018
- 2018-12-20 CN CN201811560303.3A patent/CN109479638A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101693641A (en) * | 2009-10-22 | 2010-04-14 | 南京信息工程大学 | Compound bio-fertilizer containing ardealite and application thereof |
| CN101891511A (en) * | 2010-06-15 | 2010-11-24 | 李学麒 | Organic substrate suitable for planting vegetables, melons and fruits and preparation method thereof |
| CN103503683A (en) * | 2013-10-09 | 2014-01-15 | 薛军 | Method for cultivating seedlings of purebred lycium ruthenicum with high survival rate |
| CN104396511A (en) * | 2014-11-17 | 2015-03-11 | 成都红柿子科技有限公司 | Tomato seedling breeding technology |
| CN105409381A (en) * | 2015-11-26 | 2016-03-23 | 全椒县桃花湾果蔬种植专业合作社 | Caraway seed processing method |
| CN105409524A (en) * | 2015-11-26 | 2016-03-23 | 全椒县桃花湾果蔬种植专业合作社 | Method for planting high-quality Coriandrum sativum |
| CN105594448A (en) * | 2016-03-04 | 2016-05-25 | 夏志刚 | Artificial seedling rearing method of cucumber seeds |
| CN106879442A (en) * | 2017-03-03 | 2017-06-23 | 明光现代农业科技合作推广服务中心 | A kind of utilization LED light source promotes mung bean shoot vegetable quickly to turn green method |
| CN206895295U (en) * | 2017-03-31 | 2018-01-19 | 山东农业大学 | A kind of muskmelon seedling raising supplementary lighting device for suppressing powdery mildew and promoting strong sprout |
| CN107995843A (en) * | 2017-06-01 | 2018-05-04 | 苏州佳亿达电器有限公司 | LED light compensating apparatus for green plant |
| CN107637429A (en) * | 2017-10-09 | 2018-01-30 | 广州市汇吉科技企业孵化器有限公司 | A kind of implantation methods of Silicon-rich muskmelon |
| CN108738820A (en) * | 2018-06-20 | 2018-11-06 | 内蒙古蒙草生态环境（集团）股份有限公司 | A kind of interior lawn light compensation method and indoor lawn stage lawn formation method |
Non-Patent Citations (2)
| Title |
|---|
| 徐安起等: "磁场处理种子对作物生长的影响 ", 《山东大学学报(自然科学版)》 * |
| 汪忠，高勍，李伟: "《生命密码》", 31 March 2011 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713206A (en) * | 2020-06-19 | 2020-09-29 | 四川省农业科学院植物保护研究所 | Method for increasing yield of bitter gourds by using biocontrol strain bacillus amyloliquefaciens Bam22 |
| CN114223472A (en) * | 2021-11-16 | 2022-03-25 | 广东省农业科学院动物科学研究所 | Culture method and application of high-yield cold-resistant stylosanthes guianensis |
| CN117581721A (en) * | 2024-01-19 | 2024-02-23 | 三亚市国家耐盐碱水稻技术创新中心 | Method for improving salt tolerance of rice in seedling stage by using triptfordine |
| CN117581721B (en) * | 2024-01-19 | 2024-05-07 | 三亚市国家耐盐碱水稻技术创新中心 | Method for improving salt tolerance of rice in seedling stage by using triptfordine |
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