CN117581721A - Method for improving salt tolerance of rice in seedling stage by using triptfordine - Google Patents
Method for improving salt tolerance of rice in seedling stage by using triptfordine Download PDFInfo
- Publication number
- CN117581721A CN117581721A CN202410078273.1A CN202410078273A CN117581721A CN 117581721 A CN117581721 A CN 117581721A CN 202410078273 A CN202410078273 A CN 202410078273A CN 117581721 A CN117581721 A CN 117581721A
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- China
- Prior art keywords
- rice
- seeds
- tripterygium wilfordii
- salt tolerance
- greenhouse
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 36
- 235000009566 rice Nutrition 0.000 title claims abstract description 36
- 230000015784 hyperosmotic salinity response Effects 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title claims abstract description 10
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 35
- 241000830536 Tripterygium wilfordii Species 0.000 claims abstract description 22
- 235000015398 thunder god vine Nutrition 0.000 claims abstract description 22
- 238000005507 spraying Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 11
- 150000003839 salts Chemical class 0.000 claims description 11
- 230000035784 germination Effects 0.000 claims description 10
- 239000012224 working solution Substances 0.000 claims description 10
- ZRJBHWIHUMBLCN-ZUZCIYMTSA-N LSM-5567 Chemical compound N1C(=O)C=CC2=C1C[C@H]1C(=CC)[C@]2(N)CC(C)=C1 ZRJBHWIHUMBLCN-ZUZCIYMTSA-N 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 7
- 229920000742 Cotton Polymers 0.000 claims description 5
- 244000068988 Glycine max Species 0.000 claims description 5
- 235000010469 Glycine max Nutrition 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 2
- 244000299507 Gossypium hirsutum Species 0.000 claims 1
- 239000003513 alkali Substances 0.000 abstract description 3
- 230000035882 stress Effects 0.000 description 8
- 238000011282 treatment Methods 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 6
- 241000219146 Gossypium Species 0.000 description 4
- 239000013642 negative control Substances 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 240000001606 Adenanthera pavonina Species 0.000 description 2
- 235000011470 Adenanthera pavonina Nutrition 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
Abstract
The invention belongs to the technical field of crop planting, and particularly relates to a method for improving salt tolerance of rice seedling stage by using tripterygium wilfordii, which can effectively improve salt tolerance of rice seedling stage by simple spraying under specific concentration and has important agricultural significance for planting crops in saline-alkali environment.
Description
Technical Field
The invention belongs to the technical field of crop planting, and particularly relates to a method for improving salt tolerance of rice in a seedling stage by using tripterygium wilfordii.
Background
Soil salinization is one of the most dominant abiotic stress which is harmful to agricultural production at present, and has become an important environmental factor for restricting agricultural development in China, and according to statistics, about 0.2 hundred million hm in China 2 About 20% of the cultivated land area, and causes secondary soil with the increase of industrial pollution, enlargement of irrigated and protected land areas, improper fertilizer use, etcSalinization is increasingly serious.
The rice is used as a moderate salt sensitive crop, has stronger salt tolerance in germination, tillering and maturation stages, is highly sensitive in seedling stage and reproductive stage, and the salt tolerance in seedling stage is an important influencing factor for ensuring stable yield of the rice under the condition of saline soil, and directly influences the vegetative growth and reproductive growth stages.
Therefore, how to effectively improve the salt tolerance of the rice seeds in the seedling stage has positive significance for improving the planting efficiency of the rice in the saline-alkali environment.
Disclosure of Invention
In order to achieve the above purpose, the present invention provides the following technical solutions:
the application of the chemical substance tripterygium wilfordii fordine can effectively improve the salt tolerance of rice, corn, cotton and soybean in the seedling stage under salt stress.
A regulation and control method for improving salt tolerance of rice comprises the following steps:
(1) After soaking seeds and accelerating germination of rice seeds, uniformly placing the seeds into a germination device, and culturing the seeds in a greenhouse by using a water culture nutrient solution, wherein the formula of the water culture nutrient solution refers to the recommended standard of international rice;
(2) Culturing seedlings until spraying time, and spraying leaves with the tripterygium wilfordii fordine working solution.
Further, in the step (1), the temperature of the greenhouse is controlled to be 26-30 ℃, and the sunshine duration is controlled to be 12 hours in the daytime and 12 hours in the night.
Further, in the step (2), the concentration of the tripterygium wilfordii fordine working solution is 10 -5 g/L。
Further, in the step (2), the spraying time is from 1 leaf 1 core to tillering stage of the rice.
The invention has the beneficial effects that:
the method for improving the salt tolerance of the rice in the seedling stage by using the tripterygium wilfordii is simple and convenient to operate, can effectively promote the salt tolerance of the rice in the seedling stage, and has important agricultural significance for planting crops in a saline-alkali environment.
Drawings
FIG. 1 shows phenotypes of three varieties of rice under different treatments: each culture box variety is Bo II you 15, shuang Liu you 138 and Lopa nationality red you 1564 in turn from left to right;
FIG. 2 shows the relative dry weights of individual plants of three varieties of rice under different treatments;
fig. 3 is a phenotypic difference between corn treated under salt stress and control: the left part of the figure is a seedling sprayed with the working solution of the tripterygium wilfordii, and the right part of the figure is a seedling sprayed with clear water;
fig. 4 is a phenotypic difference between cotton treated under salt stress and control: the left part of the figure is a seedling sprayed with the working solution of the tripterygium wilfordii, and the right part of the figure is a seedling sprayed with clear water;
fig. 5 is a phenotypic difference between soybean treated under salt stress and control: the left part of the figure is a seedling sprayed with the working solution of the tripterygium wilfordii, and the right part of the figure is a seedling sprayed with clear water.
Detailed Description
For a clearer understanding of the technical features, objects and effects of the present invention, specific embodiments of the present invention will be described.
Test materials: bo II you 15 rice seeds, shuang Liangyou 138 rice seeds, lopa nationality red you 1564 rice seeds, zhengdan 958 corn seeds, lu cotton grinding 28 cotton seeds and Zhonghuang 33 soybean seeds.
Example 1
Influence of spraying Tripterygium wilfordii fordine on rice seedlings during salt-free treatment
(1) After soaking and accelerating germination of three varieties of rice seeds, uniformly placing the white-exposed seeds in a germination device, and culturing in a greenhouse by using a water culture nutrient solution, wherein the formula of the water culture nutrient solution is in accordance with the recommended standard of international rice, the temperature of the greenhouse is controlled to be 26-30 ℃, the sunshine time is 12 hours in daytime, and the night is 12 hours;
(2) After 7 days of cultivation of rice seedlings, 10 -5 The working solution of the tripterygium wilfordii with the concentration of g/L is respectively sprayed to the leaves, and the sprayed clear water is used as negative control;
(3) After seedlings are continuously cultured for 8 days, counting the number of live seedlings of each treatment, weighing and measuring dry weight of all plants of each treatment after fixation and drying, and respectively calculating the relative dry weight of each variety.
The results show that when salt is not added, the tripterygium wilfordii fordine is sprayed on different varieties of seedlings, and the biomass of the tripterygium wilfordii fordine is increased (shown in figures 1-2).
Example 2
Influence of spraying Tripterygium wilfordii fordine on rice seedlings under salt stress
(1) After soaking and accelerating germination of three varieties of rice seeds, uniformly placing the white-exposed seeds in a germination device, and culturing in a greenhouse by using a water culture nutrient solution, wherein the formula of the water culture nutrient solution is in accordance with the recommended standard of international rice, the temperature of the greenhouse is controlled to be 26-30 ℃, the sunshine time is 12 hours in daytime, and the night is 12 hours;
(2) After 7 days of cultivation of rice seedlings, 10 -5 The working solution of the tripterygium wilfordii with the concentration of g/L is respectively sprayed to the leaves, and the sprayed clear water is used as negative control;
(3) The salinity of the water planting nutrient solution is regulated to be 0.6%, the water planting nutrient solution is continuously cultured in a greenhouse for 8 days (the nutrient solution is not replaced in the middle), then the number of living seedlings of each treatment is counted, all plants of each treatment are subjected to fixation and drying, then weighing is carried out to obtain dry weight, and the relative dry weight of each variety is calculated.
The result shows that under the salt stress, compared with the control, the spraying of the tripterygium wilfordii on the rice seedlings of different varieties can obviously increase the biomass of the rice seedlings, and the specific expression is as follows: under 0.6% salt stress, the dry weight of the single plant of the Luhongyou 1564 seedling in the treatment group is increased by 21% compared with the control, the dry weight of the single plant of the Shuangou 138 seedling is increased by 12%, and the dry weight of the single plant of the BoII ou 15 seedling is increased by 34% (figures 1-2).
Example 3
Influence of salt stress on corn seedlings, cotton seedlings and soybean seedlings by spraying Tripterygium wilfordii
(1) After seed soaking, uniformly placing the seeds in a germination tray, culturing in a greenhouse, wherein the temperature of the greenhouse is controlled to be 23-27 ℃, and the sunshine time is 12 hours in the daytime and 12 hours at night;
(2) After 5 days of cultivation in a greenhouse, 10 -5 Tripterygium wilfordii hook at g/L concentrationThe foodine working solution is respectively sprayed to the blades, and the sprayed water is used as negative control;
(3) The planting substrate is uniformly mixed by using an aqueous solution with the salinity of 1.0%, the seedlings are transplanted into the uniformly mixed substrate, and the result is observed after 7 days of greenhouse culture (figures 3-5).
The above examples are given for clarity of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the invention.
Claims (5)
1. The application of the chemical substance tripterygium wilfordii is characterized in that the tripterygium wilfordii can effectively improve the salt tolerance of rice, corn, cotton and soybean in the salt stress stage.
2. A regulation and control method for improving salt tolerance of rice is characterized by comprising the following steps:
(1) After soaking seeds and accelerating germination of rice seeds, uniformly placing the seeds into a germination device, and culturing the seeds in a greenhouse by using a water culture nutrient solution, wherein the formula of the water culture nutrient solution refers to the recommended standard of international rice;
(2) Culturing seedlings until spraying time, and spraying leaves with the tripterygium wilfordii fordine working solution.
3. The method according to claim 2, wherein in the step (1), the temperature of the greenhouse is controlled to be 26-30 ℃, and the sunlight time is controlled to be 12 hours in daytime and 12 hours in night.
4. The method according to claim 2, wherein in the step (2), the working solution of tripterygium wilfordii is at a concentration of 10 -5 g/L。
5. The method of claim 2, wherein in step (2), the spraying time is from 1 leaf 1 core to tillering stage of the rice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410078273.1A CN117581721A (en) | 2024-01-19 | 2024-01-19 | Method for improving salt tolerance of rice in seedling stage by using triptfordine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410078273.1A CN117581721A (en) | 2024-01-19 | 2024-01-19 | Method for improving salt tolerance of rice in seedling stage by using triptfordine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117581721A true CN117581721A (en) | 2024-02-23 |
Family
ID=89912047
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410078273.1A Pending CN117581721A (en) | 2024-01-19 | 2024-01-19 | Method for improving salt tolerance of rice in seedling stage by using triptfordine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117581721A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5468772A (en) * | 1993-03-10 | 1995-11-21 | Pharmagenesis, Inc. | Tripterinin compound and method |
| CN1826904A (en) * | 2006-04-07 | 2006-09-06 | 浙江省农业科学院 | Compound miro-emulsified pesticide of triperygium wilfordii alkaloid |
| CN105820017A (en) * | 2016-05-19 | 2016-08-03 | 四川施利旺农业科技开发有限公司 | Ecological soil conditioner for improving saline and alkaline land soil and preparation method |
| CN106070387A (en) * | 2016-06-30 | 2016-11-09 | 山东胜伟园林科技有限公司 | A kind of anti-salt alkali composition containing Radix Notoginseng and preparation method thereof |
| CN108271339A (en) * | 2014-12-30 | 2018-07-10 | 靛蓝农业公司 | Seed endophyte, compositions related and its application method between cultivar and species |
| CN109479638A (en) * | 2018-12-20 | 2019-03-19 | 顺达康（苏州）农业科技发展有限公司 | A kind of the muskmelon seedling fostering method and muskmelon seedling of high disease resistance ability |
| CN109906898A (en) * | 2019-04-02 | 2019-06-21 | 朱孔志 | Suitable for salination middle-and-low-yielding fields rice cultivating superior high-yield method |
| CN112369314A (en) * | 2021-01-06 | 2021-02-19 | 湖南杂交水稻研究中心 | Method for identifying phenotype of rice sheath blight at seedling stage |
| CN113648337A (en) * | 2021-03-26 | 2021-11-16 | 吉林大学 | A medicine for inhibiting Marek's disease virus replication and preventing or resisting Marek's disease by use of radix Tripterygii Wilfordii extract |
-
2024
- 2024-01-19 CN CN202410078273.1A patent/CN117581721A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5468772A (en) * | 1993-03-10 | 1995-11-21 | Pharmagenesis, Inc. | Tripterinin compound and method |
| CN1826904A (en) * | 2006-04-07 | 2006-09-06 | 浙江省农业科学院 | Compound miro-emulsified pesticide of triperygium wilfordii alkaloid |
| CN108271339A (en) * | 2014-12-30 | 2018-07-10 | 靛蓝农业公司 | Seed endophyte, compositions related and its application method between cultivar and species |
| CN105820017A (en) * | 2016-05-19 | 2016-08-03 | 四川施利旺农业科技开发有限公司 | Ecological soil conditioner for improving saline and alkaline land soil and preparation method |
| CN106070387A (en) * | 2016-06-30 | 2016-11-09 | 山东胜伟园林科技有限公司 | A kind of anti-salt alkali composition containing Radix Notoginseng and preparation method thereof |
| CN109479638A (en) * | 2018-12-20 | 2019-03-19 | 顺达康（苏州）农业科技发展有限公司 | A kind of the muskmelon seedling fostering method and muskmelon seedling of high disease resistance ability |
| CN109906898A (en) * | 2019-04-02 | 2019-06-21 | 朱孔志 | Suitable for salination middle-and-low-yielding fields rice cultivating superior high-yield method |
| CN112369314A (en) * | 2021-01-06 | 2021-02-19 | 湖南杂交水稻研究中心 | Method for identifying phenotype of rice sheath blight at seedling stage |
| CN113648337A (en) * | 2021-03-26 | 2021-11-16 | 吉林大学 | A medicine for inhibiting Marek's disease virus replication and preventing or resisting Marek's disease by use of radix Tripterygii Wilfordii extract |
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