CN106962195A

CN106962195A - A kind of wild cabbage seeding technique

Info

Publication number: CN106962195A
Application number: CN201710268034.2A
Authority: CN
Inventors: 苏浴源; 闫凤岐; 栗淑芳; 申领艳; 康少辉; 王文涛; 陈宝刚; 武占岭
Original assignee: ZHANGJIAKOU ACADEMY OF AGRICULTURAL SCIENCES
Current assignee: ZHANGJIAKOU ACADEMY OF AGRICULTURAL SCIENCES
Priority date: 2017-04-22
Filing date: 2017-04-22
Publication date: 2017-07-21
Anticipated expiration: 2037-04-22
Also published as: CN106962195B

Abstract

The invention discloses a kind of wild cabbage seeding technique, using following producing method for seed：Lateral bud is cut from knot, cultivated on culture medium, continuous squamous subculture obtains original maternal plant in laboratory；The breeding of sterile line：The preparation of cold bed；The preparation of seed plate；Maternal plant is heeled in；Maintainer nursery；Field planting；Pollination；Ventilation harvest：The mid-term that bears pods removes maintainer plant, and increase line space is beneficial to ventilation and penetrating light, and 70% pod is cut when turning to be yellow makes its after-ripening, threshing after drying；The breeding of male parent：Strict to select flower bud standard, pollination mark is carried out in the operation of specification cabage-type rape；Cenospecies breeding.The invention provides seeding technique, yield is up to 65 75kg/ mus, and bud rate, bud gesture are up to more than 85%, and seed production is high, to realize that wild cabbage steady production lays the first stone；After plantation, either disease resistance, winter resistance, physiology shape or yield have obvious advantage compared to other kinds, are easy to penetration and promotion to plant.

Description

A kind of wild cabbage seeding technique

Technical field

The invention belongs to vegetables seeding technique field, more particularly to a kind of wild cabbage seeding technique.

Background technology

Wild cabbage also known as cabbage, are commonly called as cabbage, belong to Cruciferae, originate in the northern bank in Mediterranean, 16th century start incoming China, is a kind of important vegetables that China various regions are generally planted, and occupies critical role in vegetable growing and supply.Its adaptability By force, easily cultivation, resistance to transport, application value is high, and edible range is wide, is vegetables cuisines indispensable on mankind's dining table, turns into Important culturing vegetable species in the world.

24,600,000 mu of whole world cultivated area, China's cabbage area accounts for whole world cultivated area up to 12,000,000 mu 48.8%.Present China generally cultivates various regions, is one of the regional spring such as northeast, northwest, North China, summer, the staple vegetable in autumn. 230,000 mu of the long-term cultivated area in Zhangjiakou, accounts for the 2% of national vegetables area.The 17%~20% of Zhan Wo cities vegetables area.Due to Zhangjiakou day and night temperature is big, and illumination is sufficient, and light quality is good, and pest and disease damage occurs less, and ecological environment is good, and wild cabbage product is sold to national each Ground, because demand is improved constantly, also increases year by year for plantation yield and seed production demand.

Research of the China to wild cabbage hybrid vigour starts from the later stage fifties in last century, and main research demonstrates half-blood There is hybrid vigour in terms of yield, resistance.In the later stage sixties to the seventies, wild cabbage selfing is systematically carried out not affine Seed selection and heterosis utilization research, and make a breakthrough, up to the present, the wild cabbage half-blood of countries in the world production All it is to be prepared using sterile line and self incompatible line.

China's research male sterile to wild cabbage starts from the initial stage eighties, to overcome self incompatible line production of hybrid seeds hybrid rate 100% can not be reached, purity is low, regularity is poor, parent degenerates from transaction for a long time, needs artificial flower bud phase to award during parent propagation Powder cost is high, the defect such as intellectual property is difficult to protect, and starts the positive male sterile breeding methods of research and utilization.

Hebei province as the important vegetables production area of China, kind in production with the Chinese Academy of Agricultural Sciences's seed selection " in sweet 21 " be The wild cabbage new varieties of representative, are to utilize in sterile line and self incompatible line Hybrid F1 generation, mainly self incompatible line parent And the numerous production of hybrid seeds of F1 cenospecies.In central plain area, using autumn and winter field sowing, Small plastic shed subculture spring flower, season in the beginning of spring crop field definite value Breeding pattern, emphasis is Xingtai area.Main producing region as China's wild cabbage, is also the leading industry of local farmers.In the presence of Problem：Season maturation etc. of blooming is produced is in same period with rainy season, haze, and continuous wet weather is unfavorable for plant blossom and pollen is passed Pollination is broadcast, disease, insect pest is added and is survived the winter through a winter, early spring largely occurs, it is impossible to ensure the stalwartness of plant, particularly side shoot Development, constitutes a serious difficulty for breeding processed.Production seed production and quality cannot be guaranteed.Yield poorly, maturity is poor, bud gesture, Bud rate is low.General 30-40kg/ mus of yield.

Number of patent application is 201410369272.9 innovation and creation, discloses specifically related to a kind of raising collard The cultural method of cut-flower amount and production of hybrid seeds amount, discloses a kind of cultural method for improving collard cut-flower amount and production of hybrid seeds amount, including Adjust sowing time, nursery, field planting, field management, harvesting cut-flower, stalk processing, leaf-head processing, in batches pollination in time, harvesting etc. Processing, the invention is simple and easy to apply, easy to operate, and effect substantially, can make cut-flower collard both to have gathered in a Fresh Cutting flower, and Fresh Cutting flower yield increase by more than 30%, moreover it is possible to dramatically increase seed production, make seed production increase income more than 20%, and also improve Seed quality, reduces production cost, adds benefit, but collard in the patent be typically used as viewing and admiring there is provided Method can not also solve cabbage lower in terms of yield, disease resistance and the production of hybrid seeds amount the problem of.

The method that the patent of invention of Application No. 94100993.9 discloses wild cabbage hybrid seeding, but per mu yield after plantation 3300-3800 kilograms, yield poorly, it is difficult to meet the market demand.

The content of the invention

In order to overcome above problems of the prior art there is provided a kind of wild cabbage seeding technique, the cabbage hybrid is Using male sterile line TM105-7 as maternal (maintainer 8513-1-2), the head cabbage varieties being formulated using F07013-5 as male parent, Using following producing method for seed：

(1) breeding of original maternal plant

Original maternal plant gene is MSMS, turns into heterozygote by foreign aid's pollination, lateral bud is cut from knot, on culture medium Culture, in laboratory, continuous squamous subculture obtains original maternal plant；

(2) breeding of sterile line

(2.1) preparation of cold bed：South-north direction cold bed, wide 2.0m, ground grading, surface laying 10cm perlites, upper berth If ground cloth, to improve ground temperature；

(2.2) preparation of seed plate：Prepare 50 cave seed plates, it is interior to be provided with seed plate matrix；

(2.3) maternal plant is heeled in：Early Febuary, the healthy and strong test tube seedling of maternal plant root leaf development is placed on greenhouse floor 5-7 days, will Bottle cap is opened to be placed 5-7 days again, is made after environment is merged completely with external environment condition in bottle, seedling is taken out, is removed the culture medium of root, The maternal plant survived is turned out in field planting in hole tray；

(2.4) maintainer nursery：When female parent heels in 10-15 days, by maintainer seed sowing in 50 cave seed plates, matrix, Cold bed, water and fertilizer management is heeled in identical with maternal plant；

(2.5) it is colonized：Late May, maternal plant：Maintainer=1:2 field planting are in open country；Line-spacing 50cm, spacing in the rows 30cm, it is maternal 3000 plants or so；Keep there is no Cruciferae application in 20km simultaneously；

(2.6) pollinate：5 casees honeybee pollination of every mu of holding at the beginning of 7 months, pollination period is kept for 1-1.5 months；

(2.7) ventilation harvest：The mid-term that bears pods removes maintainer plant, and increase line space is beneficial to ventilation and penetrating light, 70% pod hair Being cut when yellow makes its after-ripening, is put into hurricane drier, threshing after drying；

(3) breeding of male parent：Strict to select flower bud standard, pollination mark is carried out in the operation of specification cabage-type rape.

(4) cenospecies breeding

(4.1) sow

Sowed at the beginning of late Febuary to 3 months under dam in greenhouse hole tray, male parent, the maternal same period are broadcast；

(4.2) seedling management

When seedling 3-5 piece leaves, vernalization treatment will be carried out, 1-4 DEG C of bed temperature is easy when nocturnal temperature takes off straw mat with morning, is protected Card growth of seedling is of moderate size, and blade is abundant, strong stress resistance after field planting, and hybrid seed yield is high；

(4.3) field management

(4.3.1) selection of land

Selecting on dam water and fertilizer condition, guaranteedly, 2-3 did not planted the plot production of hybrid seeds of brassicaceous vegetable；；In isolation The Cabbage production field cultivated in area, if any bolting sign, should be harvested in time；

(4.3.2) site preparation

Production of hybrid seeds plot is avoided and brassicaceous vegetable continuous cropping.General mu applies that 3 tons of organic fertilizers make base fertilizer and 60 kilograms of composite fertilizers do Base manure, ploughs deeply and rakes；

(4.3.3) is colonized

Parent is pressed 1 by mid or late May-early June：2 are colonized in open country, are cultivated using high ridge, under-film drip irrigation, every mu of cultivation Plant 4500-5000 plants；

(4.3.4) watering, fertilizing：When kind of a strain bolting Post flowering, topdressed with water；

(4.3.5) adjusts the florescence：The plant of florescence deviation is caused to be adjusted the florescence to climate reasons；The mu that comes into bloom is put 5-10 case honeybees are put, supple-mentary pollination improves yield；

(4.3.6) pest control：Wild cabbage florescence is prevented and treated mainly for pest and disease damage；

(4.3.7) is harvested：When the several pods of kind of strain about half turn yellow, you can cut sowing strain in the morning；In kind of a strain after-ripening, take off Grain, clean, dry, in storage and transport process, never kind entanglement prevents mechanical admixture.

Any of the above-described scheme is preferably, and the specific propagation method of original maternal plant is in the step (1)：By lateral bud from knot Portion is cut, and is sterilized, is seeded on culture medium by arsenic mercury, produces callus；Induce young shoot differentiation；Rooting induction, in experiment The continuous squamous subculture in room obtains original maternal plant.

Any of the above-described scheme is preferably, and the culture medium is MS culture mediums, in MS+NAA 0.2mg/L+6-BA 2mg/ Young shoot differentiation is induced in L culture medium；Rooting induction is carried out in MS+NAA 0.2mg/L culture medium, laboratory it is continuous after It is commissioned to train to support and obtains original maternal plant.

Any of the above-described scheme is preferably, and the seed plate matrix in step (2.2) seed plate is import turf.

Any of the above-described scheme is preferably, and needs to keep 60% shading rate after the field planting of step (2.5) maternal plant, in strong light Shelter from heat or light 3-5 days, temperature control in 15-52 degree, plug media keep seeing it is dry see wet, per imposing within 7-10 days 0.3% special fertilizer for seedling culture 1 time.

Any of the above-described scheme is preferably, and step (2.5) field planting needs to carry out vernalization treatment before carrying out.

Any of the above-described scheme is preferably, and the concrete operation method of the vernalization treatment is：Greenhouse is maintained at temperature 0-10 DEG C, kept for 30-40 days, at the beginning of continueing to -6 months late Mays.

Any of the above-described scheme is preferably, and after step (2.5) field planting, mu applies 3 tons of high-quality farm manure, NPK 50kg。

Any of the above-described scheme is preferably, and after step (2.5) field planting, pest and disease damage is prevented and treated before flowering；When bolting Post flowering, Topdressed with water, every mu chases after 10 kilograms of urea.

Any of the above-described scheme is preferably, and the prevention and controls of pest and disease damage include：Physical control and chemical prevention.

Any of the above-described scheme is preferably, and the physical control method includes：Field hangs Yellow sticky trap or yellow plate Bar 25cm × 40cm, applies last layer machine oil, 30-40 blocks/667m thereon², trap and kill aphid.

Any of the above-described scheme is preferably, and the chemical prevention requirement was sprayed for above-mentioned insect every 5-6 days before flowering Medicine, continuous 3-4 use of spray, specific prevention and controls include：Mancozeb and/or general make every effort to overcome prevent and treat samping off, fenaminosulf And/or vertical withered spirit prevents and treats damping-off；Using new phytomycin and/or can kill and/or agricultural streptomycin sulphate prevent and treat black rot；Adopt With Affirm (Merck Co.) missible oil and/or ethiprole suspending agent and/or chlorfenapyr suspending agent preventing and treating cabbage caterpillar, diamondback moth；Using Deltamethrin milk oil and/or imidacloprid and/or Acker Thailand prevent eliminating aphis.

Any of the above-described scheme is preferably, and step (2.7) wild cabbage entered after fruiting period, to be accomplished to see to do and just be poured, and Each dissolved in water urea 100g/10m², into after the peak period that bears pods, plant is adjusted with reference to ventilation, by bottom old leaf, residual Leaf is extractd in time, increases draught capacity, reduces the incidence of disease of downy mildew, while being also noted that the aphid of spray prevention in time, harvesting When it is noted that mark.

Any of the above-described scheme is preferably, and the breeding of the male parent will be according to operation claimed below：

(14.1) strictly selects flower bud standard：The flowers are in blossom selects the bud of suitable age to carry out stripping flower bud for first 1 day；

(14.2) specifications cabage-type rape is operated：When pollinating for the first time, removed flower is opened, remove stamen and give over to Take powder to use, then carry out stripping flower bud to bud with instrument, can be pollinated during stripping flower bud with to expose column cap is advisable, and bud is caused during stripping flower bud Mechanical wounding is as far as possible small, then takes one, stamen gently to be smeared on the column cap exposed with instrument, and pollination is completed；

(14.3) carries out pollination mark：Strain, seed collecting field number, pollination order, pollination people, pollination are indicated with notice plate Time；

(12.4) covers inflorescence to copy paper bag：Bagging is carried out to the inflorescence of cabage-type rape, there are other in air-prevention Wild cabbage pollen descends slowly and lightly washes away column cap pollen after column cap up or down rain；

(14.5) removes utensil and residual pollen on hand in time：Awarding when powder is changed plant with 75% medicinal alcohol pair The position of apparatus and contamination pollen carries out chemical emasculation, prevents from artificially causing mixing for self-mating system；

(14.6) strengthens final-period management and harvesting, prevents mechanical admixture.

Any of the above-described scheme is preferably, and the step (12.1) selects the flower bud standard to be specially：Select to have bloomed from outermost A nearest circle flower bud is suitable stripping flower bud, and suitable stripping flower bud is usually no more than 10.

Any of the above-described scheme is preferably, and needs to prepare bed soil before step (4.1) sowing：According to 1:A field of 1 ratio Garden mould is mixed with a organic fertilizer become thoroughly decomposed and sieved, and every 100 kilograms of Nutrition Soils are mixed thoroughly with 0.2 kilogram of nitrogen, phosphorus, K composite fertilizer .

Any of the above-described scheme is preferably, after seedling all sprouts in step (4.2) daytime seedling all sprout after day temperature 18-22 DEG C it is advisable；The method of vernalization treatment is：Keep greenhouse temperature 0-10 DEG C, 30-40 days.

Any of the above-described scheme is preferably, in step (4.3.1), when determining production of hybrid seeds plot, need to isolate more than 20km.

Any of the above-described scheme is preferably, and organic fertilizer is thick fertilizer and barnyard manure in step (4.3.2), and 60 kilograms of composite fertilizers include 10 kilograms of 50 kilograms of NPK and potassium sulfate.

Any of the above-described scheme is preferably, and to be poured first time water after step (4.3.3) field planting, after being rewatered after bolting, be poured Sufficient water, with sharp slow seedling.

Any of the above-described scheme is preferably, and in step (4.3.4), is topdressed with water, and every mu chases after 10 kilograms or so of urea.

Any of the above-described scheme is preferably, in step (4.3.5), and the specific practice at adjustment florescence is：It is too early to bolting Parent carries out heavy pinching, promotes bottom side shoot sprouting.

Any of the above-described scheme is preferably, and the prevention and controls of wild cabbage florescence main diseases and insect pests include in step (4.3.6)： Physical control and chemical prevention.

Any of the above-described scheme is preferably, in step (3.7), and the seed not dried can not be packed or raft storage, fine day Bask seeds to make thinner on concrete floor and be exposed to the sun.

The present invention also provides a kind of wild cabbage high yield cultivating method, is cultivated using above-mentioned wild cabbage hybrid seeding, including with Lower step：

(15.1) hole plate seedling growth：In 3-5 month nursery, every mu of sowing quantity 35-50g, cave is pressed in sabot, presses acupoint depth 8-10mm, Matrix is enclosed, after planting the lid mulch film on furrow；

(15.2) managing after sowing：Mulch film is thrown off in time when young shoot breaks ground, seedling age can be colonized for 30 days or so；

(15.3) it is colonized：When seedling length to 6-7 true leaves, you can move in carrying out site preparation and fertilization before Field planting, field planting, Mu applies well-rotted farmyard manure 3000-4000 ㎏, the ㎏ of two ammonium 25, and field planting uses plum blossom-shaped high-density planting method：110cm films ridging is planted Training, earthing caudacoria wide 70cm, row spacing 40cm away from ridge side 10cm, are respectively colonized a line, spacing in the rows 30cm is fixed at four plants of adjacent centers One plant is planted, by that analogy, realizes that every mu is planted 6000 plants or so；

(15.4) harvest：It can be harvested when leaf-head wraps reality substantially, and outer layer ball leaf is shinny.

Preferably, also need to carry out hardening before step (15.3) field planting：Want low temperature hardening within 10 days or so before field planting, i.e., The ventilation of canopy room is increased, then gradually removing canopy film makes the temperature in seedbed close to outside air temperature, 2-3 days can be complete before field planting Covering is thrown off, it is adapted with being colonized the environment of field.

Preferably, the preparation method of step (15.3) well-rotted farmyard manure is：Annual June by freshly-slaughtered poultry excrement, pig manure and Sheep manure, ratio is 3:2:1, it is stored in manure pit, top covers yellow mud, film successively, fermentation 2-3 months, using high temperature by agriculture Family's fertilizer becomes thoroughly decomposed, and after becoming thoroughly decomposed, opens film, stirs, and is sprinkled after liquid bio bacterium is diluted into manure pit, is then stirred for the coming year after mixing It can be used.

Beneficial effect

The present invention provides a kind of wild cabbage seeding technique, with advantages below：

(1) provides seeding technique, and seed production is high, can make " to open sweet 40 " hybrid seed yield and reach 65-75kg/ mus, be real Existing wild cabbage steady production lays the first stone；

(2) after kinds are planted, either disease resistance, winter resistance, physiology shape or yield have significantly compared to other kinds Advantage；

(3) ensure that seed quality and quantity, meet the demand of production demonstration；

(4) is applied to multiple area plantations, is easy to penetration and promotion to plant；

(5) yield reaches 65-75kg/ mus, and bud rate, bud gesture are up to more than 85%.

Embodiment

In order to be best understood from technical scheme and advantage, below by way of embodiment, the present invention is done into One step explanation.

Embodiment 1.1

Term and definition

Sterile line:The tool that the male sterility individual plant chosen is cultivated through continuous backcross again with fertile individual hybridization There is the quality of male sterility feature and neat and consistent.

Maintainer：When hybridizing as male parent and sterile line, F1 can be made to keep the plant lines of male sterility.

Self incompatible line：Refer to complete flower and normal female, andro gamete can be formed, but lack the solid energy of self-pollination A kind of self-infertility of power.

Sweet 40 seed selection, selection is as follows:

1. sterile line breeding method and technology path

The discovery of 1.1 sterile sources and conservation

Autumn in 2003 sows " alar-85 " kind in open country, and definite value is in greenhouse then, through vernalization treatment, to colony when blooming Individual plant carries out Fertility identification, and the standard of identification is that column cap is bright-coloured, exposed, and nectary is flourishing.Flower pesticide is kept mum, and is dyed and is reflected through tabletting Fixed, flower pesticide intracavitary is without pollen.30 individual plants of primary election in more than 12000 colonies, destroy apical dominance, promote lateral bud development, offside Raw young shoot its carry out that tissue culture is asexual to reserve seed for planting.

The seed selection of 1.2 maintainers

Maintainer 8513-1-2 is the fertile plant in sterile line breeding offspring, through monoploid pollen cultures, obtains more than 30 DH systems, then the self-mating system through flower training two generations strain seed selection.

The seed selection of 1.3 sterile lines

1.3.1 homozygote sterile line breeding technology path

Wild cabbage dominant genic male sterile type is also influenceed in addition to by sterile gene control by modifier, partial male infertility There is environmental sensitivity in strain, under certain genetic background and environmental condition, and a small amount of great-hearted pollen occur in some sterile plants.With The male sterile plant of dominant sterile gene homozygosis is isolated in trace-pollen sterile plant self progeny, tissue culture propagation offspring is recycled Hybridize to be maternal with maintainer.

1.3.2 the transformation of merit

Sterile plant is first hybridized with Elite inbred, character transformation mainly is carried out to sterile line, for the bad of sterile line Character, selects the self-mating system of merit to be oriented transformation, offspring's sterility and advantage character are integrated in one.

1.3.3 cenospecies Application way and technology path

In Zhangjiakou City academy of agricultural sciences dune ridge subbase preparing hybrid combination.Summer in the same year carries out field in Zhangbei County Xi Shungou bases Between paired observation, combination TM105-7 × F07013-5 have outstanding performance.Carry out after variety test, be officially named and " open sweet 40”.Afterwards pilot production is carried out in the counties of Zhangjiakou City Ba Shang five.So far add up to promote 1219hm²。

The present invention also provides a kind of wild cabbage high yield cultivating method, and wild cabbage seeding technique and high yield cultivating method specific method are such as Under:

2nd, wild cabbage seeding technique

Opening sweet " 40 " seeding technique includes sterile line breeding, maintainer, male parent breeding, four parts of cross breeding seed, profit With the climatic superiority on local dam, under dam, work out the numerous seeding technique shown unique characteristics, i.e., nursery under dam, heel in, vernalization of surviving the winter Produce pollinated on the strain of trophosome kind, the dam production of hybrid seeds, production parent and cenospecies seed.

(1) breeding of original maternal plant

Original maternal plant gene be MSMS, itself does not have pollen, as long as have foreign aid pollination just turn into heterozygote, it is preserved with Breeding relies primarily on asexual tissue culture, lateral bud is cut from knot, on MS culture mediums, by producing callus-induction young shoot Differentiation-induction root, in laboratory, continuous squamous subculture obtains original maternal plant.

Specific method is：Lateral bud is cut from knot, sterilized by arsenic mercury, is seeded on MS culture mediums, callus group is produced Knit；In MS+NAA 0.2mg/L+6-BA 2mg/L culture medium, induction young shoot differentiation；In MS+NAA 0.2mg/L culture medium Rooting induction is carried out, continuous squamous subculture obtains original maternal plant in laboratory.

(2) breeding of sterile line

The preparation of 2.1 cold beds：The wide 2.0m of south-north direction cold bed, ground grading, surface laying 10cm perlites, upper laying Cloth, to improve low temperature.

The preparation of 2.2 seed plates：50 cave seed plates, seed plate matrix is import turf

2.3 maternal plants are heeled in：

Hardening：In Zhangjiakou region, annual early Febuary, the healthy and strong test tube seedling of maternal plant root leaf development is placed on greenhouse floor 5-7 days, bottle cap is opened and placed again 5-7 days, environment in bottle is merged completely with external environment condition.

Heel in：The cold bed for placing seed plate takes out the seedling in test tube seedling, removes the culture medium of root, is colonized in hole tray. 60% shading rate shelters from heat or light 3-5 days in strong light, temperature control in 15-52 degree, plug media keep seeing it is dry see wet, per chasing after within 7-10 days Apply 0.3% special fertilizer for seedling culture 1 time, turn out the maternal plant survived.

2.4 maintainer nursery：

When female parent heels in 10-15 days, by maintainer seed sowing in 50 cave seed plates.Matrix, cold bed, water and fertilizer management and maternal plant Heel in identical；

2.5 vernalization treatment：

At the beginning of early March to 4 months, when maternal plant, maintainer rice shoot 3-5 piece leaves, vernalization treatment will be carried out, greenhouse temperature 0- is kept 10 DEG C, 30-40 days, at the beginning of continueing to -6 months late Mays；

2.6 field planting：

Late May, maternal plant：Maintainer=1:2 field planting (height above sea level 1400m) open countrys on dam.Line-spacing 50cm, spacing in the rows 30cm, It is maternal 3000 plants or so.There is no Cruciferae application in 20km.Mu applies 3 tons of high-quality farm manure, NPK 50kg, film Lower drip irrigation cropping pattern.Water, fertilizer, pest management are identical with the production of hybrid seeds；

2.7 pollination：

5 casees honeybee pollination of every mu of holding at the beginning of 7 months, pollination period is kept for 1-1.5 months；

2.8 ventilation harvests：

The mid-term that bears pods removes maintainer plant, and increase line space is beneficial to ventilation and penetrating light.The jaundice of 70% pod is to cut to make thereafter It is ripe, hurricane drier is put into, threshing after drying；

3. the breeding of male parent：

Male parent is self incompatible line, and breeding is different from sterile line propagation for single male parent except field planting, and other are just the same, No longer repeat herein so to accomplish 6 strictly：

One is strictly to select flower bud standard：Select the bud of suitable age to carry out stripping flower bud, can prevent from selecting flower bud too small during stripping flower bud Or from bud in full bloom.The standard of qualified bud should rest in that the flowers are in blossom first 1 day, and general daily per sequence, the flowers are in blossom puts 3-4, from outermost It is suitable stripping flower bud to enclose the nearest circle flower bud that bloomed, and is usually no more than 10.

Two be the operation of specification cabage-type rape：When pollinating for the first time, removed flower is opened, remove stamen and give over to and take powder With then carrying out stripping flower bud to bud with sharp mouth tweezers.It can be pollinated with to expose column cap and be advisable during stripping flower bud, bud is caused during stripping flower bud Mechanical wounding is the smaller the better, to shorten the callus time of bud.Then one, stamen is taken in the post exposed with sharp mouth tweezers Gently smeared on head, pollination is completed.

Three be to carry out pollination mark：Strain seed collecting field number, pollination order, pollination people, pollination time are indicated with notice plate. Then notice plate is tethered on the major branch of whole strain, not contacted to earth with notice plate highly, flooded when watering less than, easily find and will not bedding bag Sub-set lives to be advisable.

Four be to cover inflorescence to copy paper bag：Suitable sack pair is selected according to plant size with different model copy paper bag Cabage-type rape inflorescence carry out set sack, with have in air-prevention other wild cabbage pollen descend slowly and lightly column cap or rain after wash away column cap flower Powder.

Five be timely to remove utensil and residual pollen on hand：When in full bloom because wild cabbage is spent, plant entirety flower density compared with Greatly, it is easy to be stained with pollen on hand with it, takes and polliniferous can also be remained on the tweezers of powder.So being used having awarded when powder is changed plant 75% medicinal alcohol carries out chemical emasculation to the position of apparatus and contamination pollen, prevents from artificial causing mixing for self-mating system.

Six be to strengthen final-period management and harvesting, prevents that mechanical admixture wild cabbage entered after fruiting period, and the increase of liquid manure demand will Accomplish to see to do and just pour, each dissolved in water urea 100g/10m².Into after the peak period that bears pods, plant is adjusted with reference to ventilation, Bottom old leaf, residual leaf are extractd in time, increase draught capacity, the incidence of disease of downy mildew is reduced, while being also noted that timely spray Prevent aphid.It is noted that mark, never mixes the seed of different labels together during harvesting.Label is lost on plant, to look for Marked again to field number.Field number and mark board are all lost, determined by field field planting figure.

4th, cenospecies breeds

4.1 sowing

Sowed at the beginning of late Febuary to 3 months under dam in greenhouse hole tray, male parent, the maternal same period are broadcast.50 hole plate seedling growths.

4.2 seedling management

18-22 DEG C of day temperature after seedling all sprouts, it is impossible to more than 26 DEG C；When seedling 3-5 piece leaves, it will carry out at vernalization Reason, 0-10 DEG C of greenhouse temperature of holding, 30-40 days, at the beginning of continueing to -6 months late Mays.Bed temperature when nocturnal temperature takes off straw mat with morning 1-4 DEG C is easy.Ensure that growth of seedling is of moderate size, blade is abundant, strong stress resistance after field planting, hybrid seed yield is high.

4.3 field management

4.3.1 selection of land selects on dam (height above sea level 1400m) water and fertilizer condition guaranteedly, when determining production of hybrid seeds plot, ordinary circumstance Under need to isolate more than 20km.In addition, wild cabbage cultivated in isolated area etc produces field, if any bolting sign, it should harvest in time. 2-3 did not planted the plot production of hybrid seeds of brassicaceous vegetable.

4.3.2 site preparation production of hybrid seeds plot avoid with brassicaceous vegetable continuous cropping, be preferably selected.General mu apply fertilizer to the subsoil 3 tons (thick fertilizer, Barnyard manure), 10 kilograms of 50 kilograms of NPK and potassium sulfate make base manure, plough deeply and rake.

4.3.3 field planting：Parent is pressed 1 by mid or late May-early June：2 are colonized in open country, typically using under high ridge, film Drip irrigation is cultivated, and first time water is poured after field planting, and after being rewatered after bolting (after the adjustment florescence), every mu is transplanted 4500-5000 plants.Pour Sufficient water, with sharp slow seedling.

4.3.4 watering, fertilizing：When kind of a strain bolting Post flowering, typically topdressed with water, every mu chases after 10 kilograms or so of urea.

4.3.5 the florescence is adjusted：The florescence deviation such as caused by climate reasons, parent that can be too early to bolting carries out heavy pinching, Promote bottom side shoot sprouting, play the effect of adjustment florescence.The mu that comes into bloom places 5-10 case honeybees, and supple-mentary pollination improves yield.

4.3.6 pest control：Wild cabbage florescence is mainly prevented and treated pest and disease damage, including physical control and chemical prevention.

Physical control method includes：Field hangs Yellow sticky trap or yellow strip 25cm × 40cm, and last layer is applied thereon Machine oil, 30-40 blocks/667m², trap and kill aphid.

Chemical prevention requirement sprayed a medicine, continuous 3-4 use of spray, tool every 5-6 days for above-mentioned insect before flowering Body prevention and controls and dispenser species are as shown in table 1.

The main diseases and insect pests of table 1 prevent and treat list

Controlling object	Pesticide name
		Samping off	Mancozeb, general make every effort to overcome
Damping-off	Fenaminosulf, vertical withered spirit
		Black rot	New phytomycin, can kill, agricultural streptomycin sulphate
Cabbage caterpillar, diamondback moth	Affirm (Merck Co.) missible oil, ethiprole suspending agent, chlorfenapyr suspending agent
		Aphid	Deltamethrin milk oil, imidacloprid, Acker are safe

4.3.7 harvest：When the several pods of kind of strain about half turn yellow, you can cut sowing strain in the morning.Kind of a strain after-ripening, threshing, Clean, dry, in storage and transport process, never kind entanglement and mechanical admixture.The seed not dried can not be packed or raft storage, fine It, which basks seeds to make thinner on concrete floor, is exposed to the sun.

The main cultivation crop that sweet " 40 " are Ji Bei is opened, development and the agriculture, rural areas and farmers industry development of variety and production of hybrid seeds industry cease Correlation, is local pillar industry, and the present invention formulates the seeding technique of wild cabbage preferably to instruct farmer development seed industry, is city Field provides elite hybrid, significant.Can solve the problem that simultaneously continuous wet weather that the southern production of hybrid seeds occurs be unfavorable for plant blossom and Pollen transmission is pollinated；Insect pest early spring largely occurs；It cannot ensure the development of the stalwartness of plant, particularly side shoot；Produce seed production Amount and quality cannot be guaranteed, and maturity is poor, the low problem of bud gesture, bud rate.

The present invention is by the above method, and seed production is high, can make " to open sweet 40 " hybrid seed yield and reach 65-75kg/ mus, yield Height, the seed of production is full, purity is high by 100%, cleanliness is high by more than 95%, germination percentage more than 85%, germinating energy more than 85%；For reality Existing wild cabbage steady production lays the first stone；After plantation, yield is high, and either disease resistance, winter resistance, physiology shape or yield are compared Other kinds have obvious advantage；This invention ensures that seed quality and quantity, meet the demand of production demonstration；It is applied to Multiple area plantations, are easy to penetration and promotion to plant.

5. the present invention also provides a kind of method that wild cabbage carries out high-yield culturing

(1) nursery

According to when ground temperature and cultivar decision nursery period.To improve planting survival rates and reducing transplanting seedling time, spring Shed or greenhouse etc. are can select during wild cabbage nursery and carries out facility hole plate seedling growth method.

(1) hole plate seedling growth：Regional March is in protecting field nursery under Bashing Area early April-the first tenday period of a month in May, dam.Every mu with kind Measure 35-50g.Using 105-128 caves/disk specification seed plate, carry after the previous day pre-processes matrix, cave is pressed in sabot in second day, presses cave Depth 8-10mm, seed is placed in cave per 1, cave, matrix is enclosed, is struck off with flat board, will sow the hole tray that completes code in a balanced way On cold bed, for improve temperature and keep humidity, after planting can on furrow lid mulch film.

(2) managing after sowing：Mulch film is thrown off in time when young shoot breaks ground, in order to avoid burn seedlings or grow high seedling.Matrix arid can spray Pour or furrow are poured.Seedling, which grows to 3 true leaves, can impose 0.3% urea.Seedling age can be colonized for 30 days or so.It should control to water before field planting And start hardening.

(2) cultivate

(1) hardening：Seedling can be colonized when growing to 6-7 piece leaves, want low temperature hardening within 10 days or so before this.Start cooling When, to make seedling by freeze injury, 5 DEG C or so not rested in typically.The ventilation of canopy room is increased first, and then gradually removing canopy film makes The temperature in seedbed can throw off covering completely in 2-3 days close to outside air temperature before field planting, it is mutually fitted with being colonized the environment of field Should.

(2) it is colonized：When seedling length to 6-7 true leaves, you can move in Field planting.Site preparation and fertilization, mu are carried out before field planting Apply well-rotted farmyard manure 3000-4000 ㎏, the ㎏ of two ammonium 25.Furrow or ridging are opened, is many typically to open bedding cultivation, the width of furrow is because of soil Physicochemical property, pour water condition and plantation density depending on, such as sand soil pours the poor plot of water condition, furrow can be made narrow and short A bit.As the viscous weight of soil property can Root growth, every ridge back two rows of cultivation.Cave is dug by distance between rows and hills dislocation, band soil sticks together the field planting of bright water during field planting. Depth is defined so that rhizome portion is buried, and by the soil compression of surrounding, so that root is contacted closely with soil, is survived with profit.It is early Spring sowing plant, can on ridge covering film.It so not only can ahead of time harvest, can also improve yield and improve product quality.Using mulch film Culture underglass, best incorporated applies weeds in herbicide preventing and treating film.Before general overlay film, every mu is led to or trefanocide 100- with dish grass 150ml is watered 50 ㎏ even sprayings in furrow face, and such as using will mix after trefanocide, spray with soil, and dish grass is logical to be mixed, directly The overlay film on furrow.(5) plantation density：Early maturing cabbage, to meet the market requirement, leaf-head weight is typically advisable with 0.8-1.0 ㎏, Therefore density dilute planting habit partially, rational close planted are changed.General line-spacing rests in 30 ㎝ or so, spacing in the rows 30-35 ㎝, every mu Strain number ensures more than 5500-6000, then 6000 ㎏ of yield/mu or so can be achieved.Field planting is highly dense using plum blossom-shaped in the present embodiment Spend the method for cultivation：110cm film Root growths, earthing caudacoria wide 70cm, row spacing 40cm, away from ridge side 10cm, are respectively colonized a line, Spacing in the rows 30cm, one plant is colonized at four plants of adjacent centers, by that analogy, realizes that every mu is planted 6000 plants or so.

(3) field management

The plot of general medium fertility, on the basis of farm manure is used sufficient, the every mu of ㎏ of urea 15-20 that spreads manuer in holes during field planting. Suitably restrain the growth of seedlings after field planting slow seedling, urea 15-20 ㎏ are imposed with reference to watering every mu after restraining the growth of seedlings.It is the prosperous of wild cabbage into the lotus throne later stage Growth period is contained, the supply of rich water is now increased, every mu chases after nitrogen, phosphorus, the ㎏ of K composite fertilizer 20.The number of dose should be according to soil Earth fertility height and plant growth condition are flexibly grasped.Keep soil moisture regime, favourable high yield.But Seedling drought resistance is strong, can Appropriate control water restraining the growth of seedlings, plays upper-controlling-lower-promoting, well developed root system effect, should accomplish that meeting drought waters after the rosette state.

(4) harvest

Spring wild cabbage should strive for early receipts, can be harvested when leaf-head wraps reality substantially, and outer layer ball leaf is shinny.If harvest is too late When, often there is blastomere, influence quality and yield.Compare neat kind, can harvest for 2-4 times.

6th, process of the test

6.1 " the seed selections of a sweet 40 " maternal male sterile line " TM105-7 "

It is dominant genic male sterile male sterile line by genetic analysis and Fertility identification TM105-7." alar-85 " kind is being introduced, Sterile plant offspring carries out continuous backcross with fertile plant and selects sterile line " BX9-3-1-5 ", because its wax is more, and development degree is big, uses " in it is sweet 11 " separation offspring self-mating systems carry out merit transformation to it.Go out fertility sterile degree through 6 generation above directive breedings 100%th, the sterile strain " TM105-7 " of sterile rate 100%.The averagely single ball weight 1.3kg of sterile line character, siphonal lobe 9-11 pieces are opened Latitude of emulsion 65.0cm, wax is light, and ball, newel 6.2cm, master pulse is green, and the micro- sweet tea of mouthfeel, pungent is low.

6.2. sweet 40 " maternal maintainer 8513-1-2 seed selection is opened

Maintainer 8513-1-2 is the fertile plant of sterile line breeding offspring, through monoploid pollen cultures, obtains more than 30 DH System, then the self-mating system through flower training two generations strain seed selection, average list ball weight 1.36kg, siphonal lobe 7-9 pieces, development degree 48.1cm, Wax is few, and ball, master pulse is dark green, and mouthfeel is micro- peppery, newel 9.5cm, with sterile line M0105-7 filial generations, sterile rate 100%, sterile degree 100%.Variable rate technology black rot resistance.

A 6.3 " sweet 40 " male parent F07013-5 seed selection

Four type origins are：1. by hybridizing new varieties cultivated etc.；2. innovation resources and seed materials, such as g13, G27, g119 etc.；3. farmers''s improved seeds, such as black leaf butch haircut, gold morning life, heart wild cabbage, sprouting broccoli, two black leaves, nanmu It is leaf, clumsy and stupid first-class；4. foreign countries introduce resource, and such as golden mu, Beijing precocity, Huang Miao, red capital precocity, early autumn, Fuji is early raw etc..

Male parent F07013-5 is " rare " self progeny's fertile plant, and the self-mating system in the continuous generation of selfing 6, single ball weighs 2.2kg, outside Leaf 9-11 pieces, development degree 58cm, wax is few, and ball, master pulse is dark green, and mouthfeel is micro- peppery, stem length 7.7cm.

7th, yielding ability is determined

The head cabbage varieties comparative test table of table 2

Note：G-1, g-2, g-3, g-4 represent sweet 20 respectively, sweet 40, sweet 50, sweet 60

As shown in Table 2, in sweet 21 be control, is planted using the implantation methods of the present invention, sweet 40 per mu yield reaches 6407.761 kgs/acre, super control 9.19% respectively, volume increase, which reaches, compares the not up to level of signifiance between the level of signifiance, kind.g- 2 rank first place, and g-4 occupies second.Do not surpass control has 2, is g-1, g-3 respectively, per mu yield up to 5518.427 kgs/acre, 5472.821 kgs/acre, than the control underproduction -6.02%, -6.80%, the underproduction is not up to the level of signifiance, and both are more also not up to The level of signifiance.The the 5th and the 6th is occupied respectively.Sweet 21 occupy the 3rd in control, and green good (ck3) is than the control underproduction -0.19%, position The 4th is occupied, first No. 4 of iron occupies the 7th than the control underproduction -8.35%.

8.1 variety comparative test

Table 3 uses PLSD methods of analysis of variance,

" the sweet annual bearing of 40 " variety test yield three of table 3

As shown in Table 3, it is control to open sweet 40 with " first No. four of iron ", carries out comparative variety in Zhangbei County Xi Shungou bases respectively Experiment, successively carries out three experiments, experiment is using the arrangement of random district's groups, 3 repetitions.By 3 continuous three years variety comparative test results of table Equally show that " sweet 40 " ratio control " iron head " volume increase reaches the level of signifiance up to 7.8%；

8.2 pilot production

" sweet 40 " the multiple spot regional testing yield result of table 4

Table 4, is carried out continuously 2 years totally 5 testing sites, respectively in Zhangjiakou City Zhangbei County, Kangbao County, Shangyi, Guyuan, Chongli five County carries out pilot production, testing site area 333m²。

It is within 2015 in sweet 21 control, test result indicates that, no matter in which test block, the yield for opening sweet 40 is above In sweet 21.High yield cultivating method using the present invention in 2016 is planted to opening sweet 40, test result indicates that, identical test area Sweet 40 yield be above opening sweet 40 yield using what commonsense method was planted in 2015.

Meanwhile, continuous two annual of multiple spot regional testing (table 4) all than control volume increase 15.7%, 18.1%, is absolutely proved " production-increasing function of the sweet 40 " kind under matched breeding technology and cultivation technique.

9. resistance screening

" sweet 40 " on dam the plantation of proving ground normal year be showed no bolting phenomenon.The serious field of continuous cropping in Chongli County A situation arises and the extent of injury is investigated, normal field management natural occurrence, without chemical control after morbidity, disease species Have：Black rot, black spot, downy mildew.Investigation method uses " Z " font method, every time 75 plants of investigation.As shown in Table 5, " opening sweet 40 " disease indexs are respectively 7.46,16.22,23.06.Show high black rot resistance, anti-black spot and resistance to downy mildew.

" sweet 40 " the disease resistance comparison sheet of table 5

10. the measure of physiological character

Dry matter content is determined using direct oven drying method；Contained using 2,6- dichloropheno-lindophenol titration measuring vitamin Cs Amount；The content of soluble protein is determined using Coomassie brilliant G-250 method

" sweet 40 " kind dry matter content 5.57mg/g, the not up to level of signifiance lower by 7.48% than control 6.02mg/g, Water content is high, and cellulose is low, and quality is fresh, tender and crisp palatability is good；Vitamin C content 0.334mg/g is higher than control 0.300mg/g 11.33%, the level of signifiance is reached, nutrition is high；The mg/g of soluble protein 0.637, it is higher by 22.50% than control 0.520mg/g, reach To the pole level of signifiance, protein nutrient is higher.(being shown in Table 6).

The physiological character comparision contents table (mg/g) of table 6

11. the measure of Agronomic trait

Maturity period is control with " first No. four of iron ", and investigation method uses " Z " font method, 15 plants of investigation every time.Measure wild cabbage Main 7 characters：Plant height, development degree, the outer number of sheets (piece), Dan Qiuchong, ball are high：Ball is wide, center pillar height, rhizome high.

As shown in table 7:" sweet 40 " kind plant height 30.19cm, than compare high, development degree 56.08cm, siphonal lobe 9-11 piece, Single ball weight 1.27kg, ball are high：Ball is wide=and 14.33:15.01st, the high 3.0cm of center pillar height 7.1cm, rhizome.

The Agronomic trait application form (cm) of table 7

Sweet 40 are can be seen that by above-mentioned contrast test, either in terms of disease resistance, winter resistance, yield, character Other kinds that compare have obvious advantage, are easy to penetration and promotion.

It should be noted that various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；Although The present invention is described in detail with reference to foregoing embodiments, it will be understood by those within the art that：It is still Technical scheme described in foregoing embodiments can be modified, or which part or all technical characteristic are carried out Equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technical side The scope of case.

Claims

1. a kind of wild cabbage seeding technique, using male sterile line TM105-7 as female parent（Maintainer 8513-1-2）, using F07013-5 as The head cabbage varieties that male parent is formulated, it is characterised in that：Using following producing method for seed：

（1）The breeding of original maternal plant

Original maternal plant gene is MSMS, turns into heterozygote by foreign aid's pollination, lateral bud is cut from knot, trained on culture medium Support, in laboratory, continuous squamous subculture obtains original maternal plant；

（2）The breeding of sterile line

（2.1）The preparation of cold bed：South-north direction cold bed, wide 2.0m, ground grading, surface laying 10cm perlites, upper laying Cloth, to improve ground temperature；

（2.2）The preparation of seed plate：Prepare 50 cave seed plates, it is interior to be provided with seed plate matrix；

（2.3）Maternal plant is heeled in：Early Febuary, the healthy and strong test tube seedling of maternal plant root leaf development is placed on greenhouse floor 5-7 days, by bottle cap Open and place again 5-7 days, make after environment is merged completely with external environment condition in bottle, seedling is taken out, remove the culture medium of root, field planting The maternal plant survived is turned out in hole tray；

（2.4）Maintainer nursery：When female parent heels in 10-15 days, by maintainer seed sowing in 50 cave seed plates, matrix, cold bed, Water and fertilizer management is heeled in identical with maternal plant；

（2.5）Field planting：Late May, maternal plant：Maintainer=1:2 field planting are in open country；Line-spacing 50cm, spacing in the rows 30cm, it is maternal 3000 plants Left and right；Keep there is no Cruciferae application in 20km simultaneously；

（2.6）Pollination：5 casees honeybee pollination of every mu of holding at the beginning of 7 months, pollination period is kept for 1-1.5 months；

（2.7）Ventilation harvest：The mid-term that bears pods removes maintainer plant, and increase line space is beneficial to ventilation and penetrating light, and the jaundice of 70% pod is Cutting makes its after-ripening, is put into hurricane drier, threshing after drying；

（3）The breeding of male parent：Strict to select flower bud standard, pollination mark is carried out in the operation of specification cabage-type rape；

（4）Cenospecies breeding

（4.1）Sowing

（4.2）Seedling management

When seedling 3-5 piece leaves, vernalization treatment will be carried out, 1-4 DEG C of bed temperature is easy when nocturnal temperature takes off straw mat with morning, it is ensured that children Seedling growth is of moderate size, and blade is abundant, strong stress resistance after field planting, and hybrid seed yield is high；

（4.3）Field management

（4.3.1）Selection of land

Selecting on dam water and fertilizer condition, guaranteedly, 2-3 did not planted the plot production of hybrid seeds of brassicaceous vegetable；；In isolated area The Cabbage production field of cultivation, if any bolting sign, should be harvested in time；

（4.3.2）Site preparation

Production of hybrid seeds plot avoid with brassicaceous vegetable continuous cropping,

General mu applies that 3 tons of organic fertilizers make base fertilizer and base manure makees in 60 kilograms of composite fertilizers, ploughs deeply and rakes；

（4.3.3）Field planting

Parent is pressed 1 by mid or late May-early June：2 are colonized in open country, are cultivated using high ridge, under-film drip irrigation, every mu of plant 4500-5000 plants；

（4.3.4）Watering, fertilizing：When kind of a strain bolting Post flowering, topdressed with water；

（4.3.5）Adjust the florescence：The plant of florescence deviation is caused to be adjusted the florescence to climate reasons；The mu that comes into bloom places 5- 10 casees honeybees, supple-mentary pollination improves yield；

（4.3.6）Pest control：Wild cabbage florescence is prevented and treated mainly for pest and disease damage；

（4.3.7）Harvest：When the several pods of kind of strain about half turn yellow, you can cut sowing strain in the morning；In kind of a strain after-ripening, threshing, clear In choosing, dry, storage and transport process, never kind entanglement prevents mechanical admixture.

2. a kind of wild cabbage cross-breeding technology as claimed in claim 1, it is characterised in that：The step（1）In original maternal plant Specific propagation method be：Lateral bud is cut from knot, is sterilized, is seeded on culture medium by arsenic mercury, callus is produced；Lure Lead young shoot differentiation；Rooting induction, in laboratory, continuous squamous subculture obtains original maternal plant.

3. a kind of wild cabbage seeding technique as claimed in claim 2, it is characterised in that：The culture medium is MS culture mediums, in MS Young shoot differentiation is induced in the mg/L of 0.2 mg/L+6-BA of+NAA 2 culture medium；In the mg/L of MS+NAA 0.2 culture medium Middle carry out rooting induction, in laboratory, continuous squamous subculture obtains original maternal plant.

4. a kind of wild cabbage seeding technique as claimed in claim 1, it is characterised in that：The step（2.2）Seed plate in seed plate Matrix is import turf.

5. a kind of wild cabbage seeding technique as claimed in claim 1, it is characterised in that：Step（2.5）Need to protect after maternal plant field planting Hold 60% shading rate, shelter from heat or light in strong light 3-5 days, temperature control at 15-52 DEG C, plug media keep seeing it is dry see wet, per 7-10 It imposes 0.3% special fertilizer for seedling culture 1 time.

6. a kind of wild cabbage seeding technique as claimed in claim 1, it is characterised in that：Step（2.5）Field planting carry out before need into Row vernalization treatment.

7. a kind of wild cabbage seeding technique as claimed in claim 6, it is characterised in that：The concrete operation method of the vernalization treatment For：Greenhouse is maintained at 0-10 DEG C of temperature, is kept for 30-40 days, at the beginning of continueing to -6 months late Mays.

8. a kind of wild cabbage seeding technique as claimed in claim 1, it is characterised in that：Step（2.5）After field planting, mu applies high-quality agriculture 3 tons of family's fertilizer, NPK 50kg.

9. a kind of wild cabbage seeding technique as claimed in claim 1, it is characterised in that：Step（2.5）After field planting, disease pest is prevented and treated Evil；When bolting Post flowering, topdressed with water, every mu chases after 10 kilograms of urea.

10. a kind of wild cabbage seeding technique as claimed in claim 9, it is characterised in that：The prevention and controls of the pest and disease damage include： Physical control and chemical prevention.