CN104770300A - Method for quickly breeding monochasma Savatieri Franch.ex Maxim seedlings - Google Patents
Method for quickly breeding monochasma Savatieri Franch.ex Maxim seedlings Download PDFInfo
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- CN104770300A CN104770300A CN201510183045.1A CN201510183045A CN104770300A CN 104770300 A CN104770300 A CN 104770300A CN 201510183045 A CN201510183045 A CN 201510183045A CN 104770300 A CN104770300 A CN 104770300A
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- 238000000034 method Methods 0.000 title claims abstract description 15
- 238000009395 breeding Methods 0.000 title claims abstract description 11
- 230000001488 breeding effect Effects 0.000 title claims abstract description 11
- 241000029411 Monochasma savatieri Species 0.000 title abstract 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 6
- 241000155182 Monochasma Species 0.000 claims description 25
- 210000002268 wool Anatomy 0.000 claims description 24
- 238000005286 illumination Methods 0.000 claims description 11
- 239000002775 capsule Substances 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 4
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- 239000005720 sucrose Substances 0.000 claims description 4
- 230000003203 everyday effect Effects 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 239000005556 hormone Substances 0.000 abstract description 2
- 229940088597 hormone Drugs 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 abstract 1
- 239000002689 soil Substances 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 description 7
- 239000005648 plant growth regulator Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000013557 Plantaginaceae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for quickly breeding monochasma Savatieri Franch.ex Maxim seedlings. According to the method, the proportion and consumption of two hormones in a culture medium are reasonably controlled, and meanwhile, accessories such as active carbon are added, so that monochasma Savatieri Franch.ex Maxim seeds are developed into seedlings; and after the seedlings are transplanted to soil, the survival rate reaches more than 95%. The method can be used for breeding massive monochasma Savatieri Franch.ex Maxim seedlings within short time to meet the production requirement, is economical and practical, greatly improves the breeding efficiency, has a good effect, and can be used as an important method for industrialized production of monochasma Savatieri Franch.ex Maxim seedlings.
Description
Technical field
The present invention relates to wool savatier monochasma herb factorial praluction technical field, particularly relate to a kind of method of fast breeding wool savatier monochasma herb seedling.
Background technology
Wool savatier monochasma herb is that Scrophulariaceae savatier monochasma herb belongs to herbaceos perennial, is born in rocky mountain region, low mountain and thick grass, tool is clearing heat and detoxicating, the effect such as cooling blood and hemostasis.Because artificial cultivation wool savatier monochasma herb is not yet successful, wild resource is excavated seriously, increasingly reduces, and urgently carries out artificial breeding, protection wild resource.
Summary of the invention
The object of the invention is the contradiction for ever-increasing wool savatier monochasma herb demand and wild resource deficiency, a kind of method of fast breeding wool savatier monochasma herb seedling is provided.
The object of the invention is to be achieved through the following technical solutions: a kind of method of method of wool savatier monochasma herb seedling fast breeding, the method is: after the seed of wool savatier monochasma herb capsule is staved seed coat, be placed in medium to cultivate, condition of culture is: temperature 25 DEG C, first under dark condition, cultivate 10-20 days, then under illumination condition, 50-60 days is cultivated, illumination condition is: illumination every day 12 hours, intensity of illumination 1500-2000lx, obtains height of seedling 3-4 cm, has the wool savatier monochasma herb seedling of more than 5 root systems; Described medium is: medium based on B5, and the sucrose of the 6-benzyl aminopurine containing 0.1mg in often liter of B5 medium, the α-naphthaleneacetic acid of 0.1mg, the active carbon of 2.0g, the agar of 3.3g and 30g, pH is 6.0.
The invention has the beneficial effects as follows: the present invention carries out tissue cultures by staving wool savatier monochasma herb seed seed coat, and rationally control two kinds of hormone dosages and mutual proportioning, and the interpolation of other auxiliary materials, promote that wool savatier monochasma herb seed is after callus phase, continue to grow into seedling, in the very rare situation of wool savatier monochasma herb seed source, greatly improve reproductive efficiency.Result shows, the method meets needed for production, can be used as the important sources that wool savatier monochasma herb produces seedling.
Embodiment
The method of a kind of fast breeding wool of the present invention savatier monochasma herb seedling, comprises the following steps:
(1) 12 medium are prepared: medium based on B5,6-benzyl aminopurine, α-naphthaleneacetic acid, active carbon, agar and sucrose (amount of each composition is as shown in table 1) is added in often liter of B5 medium, be heated to boiling, stir in heating process, after agar dissolves completely, adjust ph to 6.0, obtains medium; Equably seedling medium is sub-packed in culture vessel, while divide rim to stir, avoids active carbon to sink to the bottom, add a cover after packing; After culture vessel is added a cover, put into high-pressure sterilizing pot sterilizing, sterilising conditions is: sterilizing 20 minutes at 121 DEG C, takes out culture vessel after sterilizing, for subsequent use after cooling;
| 6-benzyl aminopurine (mg/L) | α-naphthaleneacetic acid (mg/L) | Active carbon (g/L) | Agar (g/L) | Sucrose (g/L) | B5 medium | |
| Medium 1 | 0 | 0 | 2 | 3.3 | 30 | 1L |
| Medium 2 | 0 | 0.1 | 2 | 3.3 | 30 | 1L |
| Medium 3 | 0 | 0.2 | 2 | 3.3 | 30 | 1L |
| Medium 4 | 0.1 | 0 | 2 | 3.3 | 30 | 1L |
| Medium 5 | 0.1 | 0.1 | 2 | 3.3 | 30 | 1L |
| Medium 6 | 0.1 | 0.2 | 2 | 3.3 | 30 | 1L |
| Medium 7 | 0.2 | 0 | 2 | 3.3 | 30 | 1L |
| Medium 8 | 0.2 | 0.1 | 2 | 3.3 | 30 | 1L |
| Medium 9 | 0.2 | 0.2 | 2 | 3.3 | 30 | 1L |
| Medium 10 | 0.1 | 0 | 1 | 3.3 | 30 | 1L |
| Medium 11 | 0.1 | 0.1 | 1 | 3.3 | 30 | 1L |
| Medium 12 | 0.1 | 0.2 | 1 | 3.3 | 30 | 1L |
(2) wool savatier monochasma herb capsule sterilizing: wool savatier monochasma herb capsule is cleaned up in clear water; Then aseptically, be soak 1 minute in the ethanolic solution of 75% at concentration of volume percent by capsule, take out rear sterile water wash some times, put into liquor natrii hypochloritis's sterilizing 10 minutes that concentration of volume percent is 2% again, by sterile water wash some times, put into sterile petri dish after suck dry moisture for subsequent use;
(3) inoculate: open wool savatier monochasma herb capsule epidermis, with tweezers the seed access media surface in capsule, and stave seed coat, cover tightly lid;
(4) cultivate: the culture vessel completing inoculation is moved into culturing room, and cultivation temperature is 25 DEG C.Dark culturing 10-20 days, after producing callus, is set to illumination every day 12 hours, intensity of illumination 1500-2000lx, then cultivates 50-60 days by Seed inducement by illumination condition.
Cultivate the wool savatier monochasma herb seedling obtained in above-mentioned 12 medium and be labeled as A ~ L respectively, the upgrowth situation (mean value) of 12 seedling is as shown in the table.
| Height of seedling (cm) | Root quantity | Root length (cm) | Survival rate (%) | |
| A (medium 1) | 0.2 | 1.5 | 0.2 | 10.5 |
| B (medium 2) | 2.2 | 3.3 | 0.6 | 60.0 |
| C (medium 3) | 1.8 | 3.0 | 0.4 | 50.7 |
| D (medium 4) | 2.1 | 3.5 | 0.6 | 58.0 |
| E (medium 5) | 3.8 | 5.8 | 1.1 | 96.0 |
| F (medium 6) | 3.2 | 5.0 | 1.0 | 93.2 |
| G (medium 7) | 1.9 | 3.1 | 0.5 | 59.4 |
| H (medium 8) | 3.4 | 4.6 | 0.9 | 86.0 |
| I (medium 9) | 3.0 | 4.2 | 0.7 | 80.2 |
| J (medium 10) | 2.0 | 3.2 | 0.5 | 51.3 |
| K (medium 11) | 3.6 | 5.6 | 1.0 | 95.5 |
| L (medium 12) | 3.1 | 4.7 | 0.9 | 93.0 |
According to above result of the test, No. 5 medium are cultivated the wool savatier monochasma herb seedling E survival rate that obtains up to 96.0%, average height of seedling 3.8cm, average 5.8 of root quantity, the average 1.1cm of root length, its effect is best in 12 kinds of medium.And wool savatier monochasma herb almost can not grow on No. 1 medium not adding plant growth regulator, the culture effect of No. 6, No. 9, No. 12 medium that plant growth regulator concentration is higher is also not as No. 5 medium, this also can confirm, and " a small amount of plant growth regulator can Promoting plant growth, and excessive concentration on the contrary can Developing restraint." this is known together widely.
Claims (1)
1. the method for a fast breeding wool savatier monochasma herb seedling, it is characterized in that, the method is: after the seed of wool savatier monochasma herb capsule is staved seed coat, be placed in medium to cultivate, condition of culture is: temperature 25 DEG C, first under dark condition, cultivates 10-20 days, then under illumination condition, 50-60 days is cultivated, illumination condition is: illumination every day 12 hours, intensity of illumination 1500-2000lx, obtains height of seedling 3-4 cm, has the wool savatier monochasma herb seedling of more than 5 root systems; Described medium is: medium based on B5, and the sucrose of the 6-benzyl aminopurine containing 0.1mg in often liter of B5 medium, the α-naphthaleneacetic acid of 0.1mg, the active carbon of 2.0g, the agar of 3.3g and 30g, pH is 6.0.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510183045.1A CN104770300B (en) | 2015-04-17 | 2015-04-17 | A kind of method of fast breeding wool savatier monochasma herb seedling |
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| CN201510183045.1A CN104770300B (en) | 2015-04-17 | 2015-04-17 | A kind of method of fast breeding wool savatier monochasma herb seedling |
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| CN104770300A true CN104770300A (en) | 2015-07-15 |
| CN104770300B CN104770300B (en) | 2017-06-23 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109757313A (en) * | 2019-03-12 | 2019-05-17 | 浙江省中药研究所有限公司 | A kind of method for transplanting of wool savatier monochasma herb |
| CN110495391A (en) * | 2019-07-26 | 2019-11-26 | 中国林业科学研究院亚热带林业实验中心 | A kind of sterile high efficiency seedling cultivating method of savatier monochasma herb |
| CN111247972A (en) * | 2020-03-12 | 2020-06-09 | 惠州市九惠制药股份有限公司 | Harvesting and storing method of savatier salmoides seeds |
| CN111386981A (en) * | 2020-04-10 | 2020-07-10 | 惠州市九惠制药股份有限公司 | Method for planting savatier saururus in pot |
| CN113261438A (en) * | 2021-06-08 | 2021-08-17 | 中国医学科学院药用植物研究所 | Method for promoting transplanting survival of pilose antler grass stem cutting seedlings |
| CN113678696A (en) * | 2021-04-07 | 2021-11-23 | 宜春市炳晨农业科技发展有限公司 | Seedling growing method for savatier monochasma herb |
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| CN101507415A (en) * | 2009-03-26 | 2009-08-19 | 浙江大学 | In-vitro culture method of antlerpilose grass |
| CN103004601A (en) * | 2012-12-23 | 2013-04-03 | 昆明学院 | Quick reproduction method of melasma arvense (Benth.) Hand.-Mazz. through tissue culture |
| CN104221878A (en) * | 2014-10-14 | 2014-12-24 | 南京帝道农业科技有限公司 | Rapid propagation method for suspension cell culture for savatier monochasma herb |
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- 2015-04-17 CN CN201510183045.1A patent/CN104770300B/en not_active Expired - Fee Related
Patent Citations (3)
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| CN101507415A (en) * | 2009-03-26 | 2009-08-19 | 浙江大学 | In-vitro culture method of antlerpilose grass |
| CN103004601A (en) * | 2012-12-23 | 2013-04-03 | 昆明学院 | Quick reproduction method of melasma arvense (Benth.) Hand.-Mazz. through tissue culture |
| CN104221878A (en) * | 2014-10-14 | 2014-12-24 | 南京帝道农业科技有限公司 | Rapid propagation method for suspension cell culture for savatier monochasma herb |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109757313A (en) * | 2019-03-12 | 2019-05-17 | 浙江省中药研究所有限公司 | A kind of method for transplanting of wool savatier monochasma herb |
| CN109757313B (en) * | 2019-03-12 | 2023-09-26 | 浙江省中药研究所有限公司 | Transplanting method of hairy antler grass |
| CN110495391A (en) * | 2019-07-26 | 2019-11-26 | 中国林业科学研究院亚热带林业实验中心 | A kind of sterile high efficiency seedling cultivating method of savatier monochasma herb |
| CN111247972A (en) * | 2020-03-12 | 2020-06-09 | 惠州市九惠制药股份有限公司 | Harvesting and storing method of savatier salmoides seeds |
| CN111386981A (en) * | 2020-04-10 | 2020-07-10 | 惠州市九惠制药股份有限公司 | Method for planting savatier saururus in pot |
| CN113678696A (en) * | 2021-04-07 | 2021-11-23 | 宜春市炳晨农业科技发展有限公司 | Seedling growing method for savatier monochasma herb |
| CN113678696B (en) * | 2021-04-07 | 2023-03-07 | 宜春市炳晨农业科技发展有限公司 | Seedling growing method for savatier monochasma herb |
| CN113261438A (en) * | 2021-06-08 | 2021-08-17 | 中国医学科学院药用植物研究所 | Method for promoting transplanting survival of pilose antler grass stem cutting seedlings |
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Granted publication date: 20170623 |