CN109757313A - A kind of method for transplanting of wool savatier monochasma herb - Google Patents
A kind of method for transplanting of wool savatier monochasma herb Download PDFInfo
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Abstract
The present invention relates to a kind of method for transplanting of wool savatier monochasma herb, belong to wool savatier monochasma herb artificial cultivation production technical field.Method for transplanting of the present invention obtains seedling after hardening the following steps are included: by 2~4 months after germination wool savatier monochasma herb test tube seedling hardenings；Enter seedling replanting after obtained hardening to carry out nursery in seedling medium；Humidity, intensity of illumination are controlled after transplanting；Finally carry out field-transplanting.Method for transplanting of the present invention can enhance test tube seedling to the adaptability of open environment, improve the transplanting survival rate for cultivating wool savatier monochasma herb test tube seedling.
Description
Technical field
The present invention relates to wool savatier monochasma herb artificial cultivation production technical fields, and in particular to a kind of transplanting of wool savatier monochasma herb
Method.
Background technique
Wool savatier monochasma herb is Scrophulariaceae savatier monochasma herb category herbaceos perennial, is born in sandy mountainous region on the sunny side and thick grass, is had
Clearing heat and detoxicating, cooling blood and hemostasis and other effects.The tissue cultures test tube seedling of wool savatier monochasma herb and the raising technology of seed asepsis test tube seedling
It is mature, but the outdoor cultivation of test tube seedling is broken through not yet, cannot fully achieve artificial cultivation, wool savatier monochasma herb related industry
Development be restricted.
Summary of the invention
The purpose of the present invention is to provide a kind of method for transplanting of wool savatier monochasma herb.Method for transplanting of the present invention can increase
Strong test tube seedling improves the transplanting survival rate for cultivating wool savatier monochasma herb test tube seedling to the adaptability of open environment.
The present invention provides a kind of method for transplanting of wool savatier monochasma herb, comprising the following steps:
1) by the wool savatier monochasma herb 2~4 months after germination, with 2~6 true leaves with white pubescence, 3 or more root systems
Test tube seedling hardening obtains seedling after hardening；The condition of the hardening includes: that the aqueous solution containing fungicide is poured into culture bottle,
The liquid level thickness of the aqueous solution containing fungicide is 0.3cm or less；
2) enter seedling replanting after hardening that step 1) obtains to carry out nursery in seedling medium, pour nursery liquid；It is described to educate
Seedling matrix includes leaf mould, peat soil, broken bog moss and perlite；The nursery liquid includes 1/ without organic constituents
4MS aqueous solution；
3) 1~10 day after transplanting, control humidity is 90~100%, intensity of illumination is 2000~3000lux；
4) 11~30 days after transplanting, control humidity is 80~100%, intensity of illumination is 3000~5000lux；
5) after transplanting 30 days, 60% or more humidity of control, intensity of illumination are 5000~7000lux；
6) field-transplanting is carried out after transplanting 60 days.
Preferably, the time of the step 1) hardening is 7~12 days.
Preferably, the step 1) fungicide includes fludioxonil, and the mass fraction of the fludioxonil in water is 0.01
~0.05%.
Preferably, leaf mould, peat soil, the volume ratio for being crushed bog moss and perlite in the step 2) seedling medium
For 2.5~3.5:2:1:0.5.
Preferably, the step 2) seedling medium is sterilized using advance horizontal high voltage.
Preferably, after transplanting, nursery liquid was sprayed every 2~3 days.
Preferably, after transplanting, temperature is controlled at 20~28 DEG C.
The present invention provides a kind of method for transplanting of wool savatier monochasma herb.Method for transplanting of the present invention by reasonable hardening,
Environmental condition after suitable transplanting medium and control transplanting is selected, so that test tube seedling is transplanted under open-air atmosphere survival rate up to 90%
More than.The method of the present invention is able to solve the bottleneck problem of wool savatier monochasma herb artificial cultivation, and enhancing test tube seedling fits open environment
Should be able to power, improve cultivate wool savatier monochasma herb test tube seedling transplanting survival rate.
Specific embodiment
The present invention provides a kind of method for transplanting of wool savatier monochasma herb, comprising the following steps:
1) by the wool savatier monochasma herb 2~4 months after germination, with 2~6 true leaves with white pubescence, 3 or more root systems
Test tube seedling hardening obtains seedling after hardening；The condition of the hardening includes: that the aqueous solution containing fungicide is poured into culture bottle,
The liquid level thickness of the aqueous solution containing fungicide is 0.3cm or less；
2) enter seedling replanting after hardening that step 1) obtains to carry out nursery in seedling medium, pour nursery liquid；It is described to educate
Seedling matrix includes leaf mould, peat soil, broken bog moss and perlite；The nursery liquid includes 1/ without organic constituents
4MS aqueous solution；
3) 1~10 day control humidity is 90~100% after transplanting, intensity of illumination is 2000~3000lux；
4) 11~30 days after transplanting, control humidity is 80~100%, intensity of illumination is 3000~5000lux；
5) after transplanting 30 days, 60% or more humidity of control, intensity of illumination are 5000~7000lux；
6) field-transplanting is carried out after transplanting 60 days.
The present invention is by the wool deer 2~4 months after germination, with 2~6 true leaves with white pubescence, 3 or more root systems
Fine and soft grass test tube seedling hardening, obtains seedling after hardening；The condition of the hardening includes: to pour into culture bottle containing the water-soluble of fungicide
Liquid, the liquid level thickness of the aqueous solution containing fungicide are 0.3cm or less.In the present invention, the root long of the test tube seedling is preferred
More than 2cm.In the present invention, the test tube seedling preferably includes two panels cotyledon.In the present invention, 2 months pervious kind after germination
Sub- tissue-cultured seedling, excessively young tender, survival rate is lower than 10%, and test tube seedling postmaturity in 4 months or more adapts to external environment after germination
Ability dies down, survival rate decline.The present invention does not have special restriction to the cultural method of the test tube seedling, using art technology
Routine wool savatier monochasma herb test tube seeding cultivating method known to personnel.In the present invention, for cultivating the training of the test tube seedling
Feeding base is preferably the 1/2MS culture medium of pH=7, agar 5g/L, active carbon 1g/L.In the present invention, the time of the hardening is excellent
It is selected as 7~12 days, more preferably 10 days.In the present invention, the fungicide includes fludioxonil, the matter of the fludioxonil in water
Measuring score is 0.01~0.05%, more preferably 0.03%.In the present invention, the aqueous solution containing fungicide is using preceding excellent
Choosing carries out high pressure sterilization, and the present invention is not particularly limited the autoclaved condition, is using conventional high-pressure sterilising conditions
It can.After the aqueous solution containing fungicide is added in test tube seedling of the present invention, preferably open culture.Water of the present invention containing fungicide
Solution can be such that original aseptic culture medium and outside air completely cuts off, and inhibit the quick breeding of fungi, bacterium, excessively high fungicide
Concentration can inhibit the growth of wool savatier monochasma herb seedling, excessively high liquid level to be equally unfavorable for surviving for wool savatier monochasma herb seedling, this hair
The bright aqueous solution containing fungicide can make seedling percent high, and growth conditions are good.
It obtains after hardening after seedling, the present invention, which enters seedling replanting after hardening, carries out nursery in seedling medium, pour nursery
Liquid；The seedling medium includes leaf mould, peat soil, broken bog moss and perlite；The nursery liquid includes being free of organic matter
The 1/4MS aqueous solution of component.In the present invention, the seedling raising process preferably carries out in the greenhouse.Seedling medium of the present invention
Specific composition humus content is high, good water permeability and Water-saving effect are good, can guarantee higher survival rate.In the present invention,
Leaf mould in the seedling medium, peat soil, broken bog moss and perlite volume ratio be 2.5~3.5:2:1:0.5, preferably
For 3:2:1:0.5.In the present invention, the seedling medium is sterilized using advance horizontal high voltage.The present invention is to the high pressure sterilization
Condition be not particularly limited, using conventional high-pressure sterilising conditions.Seedling medium after sterilizing need not dry after letting cool, and protect
It holds wet；Seedling medium of the present invention contains the humus of higher proportion, contains the more micro- life of nocuousness in humus
Object and worm's ovum can reduce seedling cultivation bed pest and disease damage after high pressure sterilization and occur.Present invention seedling replanting after hardening enters nursery base
The pouring that nursery liquid is carried out in matter, is poured to water saturation.After transplanting, the present invention preferably sprayed nursery liquid every 2~3 days, kept
Seedling medium is wet.In the present invention, the pH value of the nursery liquid is preferably 7.0.In the present invention, the nursery liquid does not contain
Machine object component not only contributes to test tube seedling from tissue culture environment to open environment transition, moreover it is possible to reduce varied bacteria growing in seedling medium.
In the present invention, after transplanting, temperature is controlled at 20~28 DEG C.The present invention is in the incubation after transplanting, preferably
Keep ventilation.
1~10 day after transplanting, present invention control humidity is 90~100%, intensity of illumination is 2000~3000lux；
11~30 days after transplanting, present invention control humidity is 80~100%, intensity of illumination is 3000~5000lux；
After transplanting 30 days, the present invention controls 60% or more humidity, intensity of illumination is 5000~7000lux；
After transplanting 60 days, field-transplanting is carried out.
A kind of method for transplanting of wool savatier monochasma herb of the present invention is done further in detail combined with specific embodiments below
Introduction, technical solution of the present invention includes but is not limited to following embodiment.
Embodiment 1
It takes after germinateing 2 months, carries out transplanting culture using the test tube seedling that wool pilose antler grass seed carries out tissue cultures, cultivate
Base is 1/2MS, pH=7, agar 5g/L, active carbon 1g/L, and tissue-cultured seedling has 2 cotyledons, 2~4 true leaves containing white fluff, 3
~4 roots more than 2cm long.
The solution of 0.01% fludioxonil is configured with sterile water, 121 DEG C of autoclave sterilization 15min obtain germicidal solution, will
Germicidal solution pours into the culture bottle of above-mentioned test tube seedling, open wide upper cover tissue culture room cultivate 7 days, keep liquid level 0.3cm with
Under.
Seedling after hardening is subjected to greenhouse transplanting and carries out nursery, prepares leaf mould, peat soil, perlite, broken bog moss
The culture medium of=2.5:2:1:0.5 (volume ratio), 121 degrees Celsius of high pressure sterilization 15min obtain seedling medium, stand-by after cooling.
Seedling after hardening is carefully taken out, is transplanted in above-mentioned seedling medium, damage seedling rhizome is careful not to.
1/4MS, pH=7.0 aqueous solution for being free of organic constituents, i.e. nursery liquid are poured after transplanting, keep seedling cultivation bed
It is wet.Nursery liquid was sprayed every 2 days, the humidity for keeping seedling medium to moisten external environment controls from high to low: 1~10 after transplanting
It, controlling warm indoor humidity is 90~100%；11~30 days after transplanting, controlling warm indoor humidity is 80~100%；Transplanting 30
After it, warm 60% or more indoor humidity is cultivated in control.The illuminance degree of external environment controls from low to high: 1~10 day after transplanting,
The indoor intensity of illumination of control temperature is 2000~3000lux；11~30 days after transplanting, the indoor intensity of illumination of control temperature is 3000
~5000lux；After transplanting 30 days, the indoor intensity of illumination of control temperature is 5000~7000lux.Ambient temperature maintains 20
~28 DEG C, wool savatier monochasma herb seedling adapts to open environment after being kept for aerlbic culture culture 60 days, and final transplanting survival rate reaches
90%, 2~4 band villus tender leafs are newly grown after seedling replanting, can carry out field-transplanting.
Embodiment 2
It takes after germinateing 3 months, carries out transplanting culture using the test tube seedling that wool pilose antler grass seed carries out tissue cultures, cultivate
Base is 1/2MS, pH=7, agar 5g/L, active carbon 1g/L, and tissue-cultured seedling has 2 cotyledons, 2~6 true leaves containing white fluff, 3
~4 roots more than 2cm long.
The solution of 0.01% fludioxonil is configured with sterile water, 121 DEG C of autoclave sterilization 15min obtain germicidal solution, will
Germicidal solution pours into the culture bottle of above-mentioned test tube seedling, open wide upper cover tissue culture room cultivate 7 days, keep liquid level 0.3cm with
Under.
Seedling after hardening is subjected to greenhouse transplanting and carries out nursery, prepares leaf mould, peat soil, perlite, broken bog moss
The culture medium of=3.5:2:1:0.5 (volume ratio), 121 degrees Celsius of high pressure sterilization 15min obtain seedling medium, stand-by after cooling.
Seedling after hardening is carefully taken out, is transplanted in above-mentioned seedling medium, damage seedling rhizome is careful not to.
1/4MS, pH=7.0 aqueous solution for being free of organic constituents, i.e. nursery liquid are poured after transplanting, keep seedling cultivation bed
It is wet.Nursery liquid was sprayed every 3 days, kept seedling medium wet
The humidity of external environment controls from high to low: 1~10 day after transplanting, controlling warm indoor humidity is 90~100%；It moves
11~30 days after cultivation, controlling warm indoor humidity is 80~100%；After transplanting 30 days, warm 60% or more indoor humidity is cultivated in control.
The illuminance degree of external environment controls from low to high: 1~10 day after transplanting, the indoor intensity of illumination of control temperature is 2000~
3000lux；11~30 days after transplanting, the indoor intensity of illumination of control temperature is 3000~5000lux；After transplanting 30 days, control temperature
Indoor intensity of illumination is 5000~7000lux.Ambient temperature maintains 20~28 DEG C, keeps aerlbic culture culture
Wool savatier monochasma herb seedling adapts to open environment after 60 days, and final transplanting survival rate newly grows 2~4 bands after seedling replanting up to 92%
Villus tender leaf can carry out field-transplanting.
Embodiment 3
It takes after germinateing 3 months, carries out transplanting culture using the test tube seedling that wool pilose antler grass seed carries out tissue cultures, cultivate
Base is 1/2MS, pH=7, agar 5g/L, active carbon 1g/L, and tissue-cultured seedling has 2 cotyledons, 2~6 true leaves containing white fluff, 3
~4 roots more than 2cm long.
The solution of 0.01% fludioxonil is configured with sterile water, 121 DEG C of autoclave sterilization 15min obtain germicidal solution, will
Germicidal solution pours into the culture bottle of above-mentioned test tube seedling, open wide upper cover tissue culture room cultivate 7 days, keep liquid level 0.3cm with
Under.
Seedling after hardening is subjected to greenhouse transplanting and carries out nursery, prepares leaf mould, peat soil, perlite, broken bog moss
The culture medium of=3:2:1:0.5 (volume ratio), 121 degrees Celsius of high pressure sterilization 15min obtain seedling medium, stand-by after cooling.
Seedling after hardening is carefully taken out, is transplanted in above-mentioned seedling medium, damage seedling rhizome is careful not to.
1/4MS, pH=7.0 aqueous solution for being free of organic constituents, i.e. nursery liquid are poured after transplanting, keep seedling cultivation bed
It is wet.Nursery liquid was sprayed every 2 days, kept seedling medium wet
The humidity of external environment controls from high to low: 1~10 day after transplanting, controlling warm indoor humidity is 90~100%；It moves
11~30 days after cultivation, controlling warm indoor humidity is 80~100%；After transplanting 30 days, warm 60% or more indoor humidity is cultivated in control.
The illuminance degree of external environment controls from low to high: 1~10 day after transplanting, the indoor intensity of illumination of control temperature is 2000~
3000lux；11~30 days after transplanting, the indoor intensity of illumination of control temperature is 3000~5000lux；After transplanting 30 days, control temperature
Indoor intensity of illumination is 5000~7000lux.Ambient temperature maintains 20~28 DEG C, keeps aerlbic culture culture
Wool savatier monochasma herb seedling adapts to open environment after 60 days, and final transplanting survival rate newly grows 3~4 bands after seedling replanting up to 90%
Villus tender leaf can carry out field-transplanting.
Compare data
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of method for transplanting of wool savatier monochasma herb, comprising the following steps:
1) by the wool savatier monochasma herb test tube 2~4 months after germination, with 2~6 true leaves with white pubescence, 3 or more root systems
Seedling hardening obtains seedling after hardening；The condition of the hardening includes: that the aqueous solution containing fungicide is poured into culture bottle, described
The liquid level thickness of aqueous solution containing fungicide is 0.3cm or less；
2) enter seedling replanting after hardening that step 1) obtains to carry out nursery in seedling medium, pour nursery liquid；The nursery base
Matter includes leaf mould, peat soil, broken bog moss and perlite；The nursery liquid includes the 1/4MS water without organic constituents
Solution；
3) 1~10 day after transplanting, control humidity is 90~100%, intensity of illumination is 2000~3000lux；
4) 11~30 days after transplanting, control humidity is 80~100%, intensity of illumination is 3000~5000lux；
5) after transplanting 30 days, 60% or more humidity of control, intensity of illumination are 5000~7000lux；
6) field-transplanting is carried out after transplanting 60 days.
2. the method according to claim 1, wherein the time of the step 1) hardening is 7~12 days.
3. described to cough up bacterium the method according to claim 1, wherein the step 1) fungicide includes fludioxonil
The mass fraction of nitrile in water is 0.01~0.05%.
4. the method according to claim 1, wherein leaf mould in the step 2) seedling medium, peat soil, broken
The volume ratio of broken bog moss and perlite is 2.5~3.5:2:1:0.5.
5. method according to claim 1 or 4, which is characterized in that the step 2) seedling medium is high using preceding progress
Pressure sterilizing.
6. the method according to claim 1, wherein spraying nursery liquid every 2~3 days after transplanting.
7. the method according to claim 1, wherein temperature is controlled at 20~28 DEG C after transplanting.
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| CN201910185070.1A CN109757313B (en) | 2019-03-12 | 2019-03-12 | Transplanting method of hairy antler grass |
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| CN201910185070.1A CN109757313B (en) | 2019-03-12 | 2019-03-12 | Transplanting method of hairy antler grass |
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Cited By (6)
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| CN110352804A (en) * | 2019-08-15 | 2019-10-22 | 江西环境工程职业学院 | A kind of acclimatization and transplants method of savatier monochasma herb |
| CN111226704A (en) * | 2020-03-12 | 2020-06-05 | 惠州市九惠制药股份有限公司 | Cutting seedling raising method for savatier shawense |
| CN111386981A (en) * | 2020-04-10 | 2020-07-10 | 惠州市九惠制药股份有限公司 | Method for planting savatier saururus in pot |
| CN113229046A (en) * | 2021-06-08 | 2021-08-10 | 哈尔滨市康隆药业有限责任公司 | Method for promoting cultivation survival of cornua cervi pantotrichum in field |
| CN113678696A (en) * | 2021-04-07 | 2021-11-23 | 宜春市炳晨农业科技发展有限公司 | Seedling growing method for savatier monochasma herb |
| CN113812337A (en) * | 2021-04-02 | 2021-12-21 | 宜春市炳晨农业科技发展有限公司 | Hydroponic rooting method for savatier monochasma |
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| CN110352804A (en) * | 2019-08-15 | 2019-10-22 | 江西环境工程职业学院 | A kind of acclimatization and transplants method of savatier monochasma herb |
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| CN111386981A (en) * | 2020-04-10 | 2020-07-10 | 惠州市九惠制药股份有限公司 | Method for planting savatier saururus in pot |
| CN111386981B (en) * | 2020-04-10 | 2022-03-18 | 惠州市九惠制药股份有限公司 | Method for planting savatier saururus in pot |
| CN113812337A (en) * | 2021-04-02 | 2021-12-21 | 宜春市炳晨农业科技发展有限公司 | Hydroponic rooting method for savatier monochasma |
| CN113678696A (en) * | 2021-04-07 | 2021-11-23 | 宜春市炳晨农业科技发展有限公司 | Seedling growing method for savatier monochasma herb |
| CN113678696B (en) * | 2021-04-07 | 2023-03-07 | 宜春市炳晨农业科技发展有限公司 | Seedling growing method for savatier monochasma herb |
| CN113229046A (en) * | 2021-06-08 | 2021-08-10 | 哈尔滨市康隆药业有限责任公司 | Method for promoting cultivation survival of cornua cervi pantotrichum in field |
| CN113229046B (en) * | 2021-06-08 | 2023-03-31 | 哈尔滨市康隆药业有限责任公司 | Method for promoting cultivation survival of cornua cervi pantotrichum in field |
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