CN110574665A - Outdoor transplanting method for sealwort tissue culture seedlings - Google Patents
Outdoor transplanting method for sealwort tissue culture seedlings Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention relates to the field of cultivation of polygonatum sibiricum, in particular to a polygonatum sibiricum tissue culture seedling transplanting method. The method takes sealwort tissue culture as a material, and promotes survival efficiency of tissue culture seedlings through a series of measures such as transplanting matrix selection and disinfection, transplanting container and medicament disinfection, post-transplanting seedling stage environment and water management and the like. The sealwort transplanting technology provided by the invention is adopted for production, the method is simple and convenient, the medicinal value is improved, the transplanting survival rate is high, the production cost can be reduced, and the method has important significance for protecting medicinal species.
Description
Technical Field
The invention relates to the technical field of planting, in particular to an outdoor transplanting method of polygonatum sibiricum tissue culture seedlings.
Background
Polygonatum sibiricum (Polygonatum sibiricum Delar. ex Redoute) is also called Polygonatum sibiricum, yellow-cut corium gallicum, and the like, belongs to perennial herbaceous plants of Liliaceae (Liliaceae) Polygonatum (Polygonatum Mill.), and is a specific plant in southern China. The rhizome is cylindrical and the stem climbs. The impeller is raw, 4-6 pieces are taken per wheel, the strip shape is in a needle shape, and the tip of the strip shape is fist-shaped or bent into a hook. Berry is black. The flowering period is 5-6 months, and the fruit period is 8-9 months. Growing in the area with elevation of 500-1200 m, has the characteristics of liking yin, cold resistance and afraid of drought, and grows in mountain shrubs and forest edge grass in the presence of yin and dampness. Rhizoma Polygonati has the effects of moistening lung, nourishing yin, invigorating spleen, replenishing qi, invigorating kidney, and replenishing essence. Rhizoma Polygonati is also an antibacterial medicine, and has certain inhibitory effect on typhoid bacillus, tubercle bacillus, staphylococcus aureus and dermatophyte. In recent years, rhizoma polygonati products have been widely applied to coronary heart disease, hypertension, diabetes, pulmonary tuberculosis and aplastic anemia.
The sealwort seeds are difficult to collect, so that the seed germination rate is low, the seedling culture time is long, and the manual large-area planting of the sealwort is not facilitated. At present, the propagation of sealwort mainly depends on the vegetative propagation of rhizomes, but the propagation coefficient of the method is low, the use amount of the rhizomes is large, the sealwort is easy to deteriorate and rot, and the seed source is greatly limited.
Disclosure of Invention
in order to solve the technical problems, the invention provides the sealwort tissue culture seedling outdoor transplanting method, and provides a method which is simple and convenient to operate, high in growth speed and high in emergence rate by simplifying the transplanting mode and adjusting the management mode of the transplanting period.
the technical scheme of the invention is as follows:
An outdoor transplanting method of sealwort tissue culture seedlings, which comprises the following steps:
s1: transplanting the substrate, transplanting the test-tube plantlet and pre-treating the plug tray:
S2: and (3) management in a transplanting period:
S3, transplanting the plug seedlings to the outdoor after 25-30 days;
And finishing the outdoor transplantation of the sealwort tissue culture seedlings.
Preferably, the transplanting matrix in the step S1 comprises the following components in parts by weight: 1.3 to 1.9 parts of peat, 4.5 to 5.5 parts of river sand, 2.5 to 3.5 parts of bran coat and 1.5 to 2.5 parts of cottonseed hull. The peat soil is used because it contains a large amount of organic matters, is loose, has good air and water permeability, strong water and fertilizer retention capacity and light weight. Can quickly absorb water, keep moist and provide sufficient water for the root system. The bran coat contains abundant vitamins and microelements, and has good tonifying effect on plants. The main component of the bran coat is oryzanol, and the bran coat can be used for adjusting the rapid growth of the rhizoma polygonati tubers. The cottonseed hull is rich in protein, and some cotton seed fragments are mixed with the cottonseed hull. The cottonseed hulls can be used after being added with water and fermented at high temperature for 1 month, and are rich in various nutritional ingredients, namely CF 37-48%, lignin 29-32% and pentosan 22-25%; the cotton seed pieces contain CP 39%, EE 33.2%, saccharides 14.8%, and CF 2.2%. The natural culture medium prepared from the cottonseed hulls and the cottonseed fragments has a pH value close to neutral, is rich in nutrients, can retain water and ventilate, and is suitable for culturing various aerobic plants; in addition, the cottonseed hull contains 29% -32% of lignin, and can be carbonized and activated to prepare activated carbon. In a word, the cottonseed hulls have the advantages of rich nutrition, convenient material acquisition, low cost and wide application effect. The river sand, the bran coat and the cottonseed hull are mixed to increase the ventilation, so that redundant water is removed in time, the rooting is facilitated, and the outdoor transplantation of the sealwort tissue culture seedlings is facilitated. The bran coat and the cottonseed hull can also improve the slow release performance of the fertilizer and accelerate the growth of plants. The river sand particles are smooth and have quick water draining, thereby being beneficial to rooting, being suitable for outdoor growth and improving the survival rate.
preferably, the transplanting matrix processing method in step S1 is: the substrate was wetted with 0.1% aqueous potassium permanganate solution and covered with a plastic film for 24 hours. The purpose of this is to disinfect the substrate more thoroughly, and to eliminate the plant diseases and insect pests in the local environment, so as to avoid affecting other environments. Before the substrate is matched, cotton seed hulls or rice bran with dust removed are put into a white cloth bag for high-temperature sterilization, and then the white cloth bag is put into a plastic basin soaked by 0.1% potassium permanganate aqueous solution for natural cooling for standby. Removing impurities from river sand collected by a river edge by using a 12-hole iron sieve, then carrying out outdoor exposure for one week, turning over twice every day during exposure, and then subpackaging the river sand in a clean bag to be stored indoors for later use. And weighing and proportioning according to the number of the transplanted seedlings.
Preferably, the specific method for sterilizing the transplanted test-tube plantlet in the step S1 is as follows: rinsing a culture medium on a seedling in clear water, soaking for 5-10 minutes by using 2% sodium hypochlorite, soaking for 10-15 minutes by using 50% ABT (mixture of indolehexanoic acid and indolebutyric acid) aqueous solution, fishing out the seedling, sucking water by using sterile paper, transplanting, wherein the water for cleaning the seedling is tap water which is not disinfected and sterilized, and the seedling is prevented from being infected by plant diseases and insect pests by soaking in 2% sodium hypochlorite aqueous solution to influence the survival rate; the seedling is easy to be wetted with the substrate and is not easy to operate.
Preferably, the step S1 method for managing the transplanting period includes: soaking the hole tray for 24 hours by using 1000 times of 0.1% potassium permanganate aqueous solution before transplanting, then improving the nano-film technology to apply to transplanting, automatically controlling the humidity to be more than 65% -85%, controlling the temperature to be within the range of 5-25 ℃, controlling the illumination intensity to be 1800-2500 Lx, carrying out outdoor transplanting in 25-30 days, selecting rainy days during transplanting, putting under tree shade or under a shading net for seedling revival after transplanting, and gradually adapting to the outdoor environment. Immediately spraying 1000 times carbendazim solution on the hole tray after transplanting, arching by using a plastic film, controlling the humidity to be more than 65-85% and the temperature to be within 15-25 ℃, controlling the illumination intensity to be 1800-2500 Lx in the daytime, and removing the plastic film in 25-30 days. The small arched shed is arranged to provide the most suitable growth environment for the seedlings and improve the survival rate and the growth speed of the seedlings. In winter, the humidity and the temperature of the greenhouse generally cannot reach required values, the plastic film is used for covering, watering can be kept inside to keep the humidity, and the electric heating pieces are added, so that the local heating is faster; in summer, the temperature can be reduced by covering the sunshade net, and the illumination intensity is reduced. The rooting period of the tissue culture seedling is about 30 days, and the tissue culture seedling is transplanted outdoors at variable time to ensure the survival rate of the seedling.
Preferably, in the step S2, the disease control in the transplanting stage management: observing the growth condition and the surrounding environment of the plant every day after transplanting, brushing the plant with phosphorus-containing washing powder water or brushing the plant with water soaked by cigarette ends for several times when aphids are found until no aphids appear, mainly preventing the aphids, controlling humidity, cleaning the plant with the diseases when the diseases occur, deeply turning over soil for insolation, and applying potash fertilizer to improve the resistance; when snails are found, spraying Wolk star on the peripheral water source area can prevent the snails.
Fertilizing in the transplanting period management of the step S2: when transplanting, 10-15 cooked soybeans are placed at the bottom of the pot, the thoroughly decomposed cake fertilizer water is poured after 25 days, and meanwhile, water-soluble fertilizers and foliar fertilizers are sprayed in a crossed mode, wherein the water-soluble fertilizers can be 20-8-28TE water-soluble fertilizers (high-nitrogen potassium type major element water-soluble fertilizers 20-8-28+ TE-Gadobang).
Preferably, the foliar fertilizer comprises the following components in parts by weight:
P2O58-12 parts, K2O18-22 parts, Mg0.12-0.17 part, S0.15-0.22 part, Cu0.03-0.06 part, Fe0.05-0.15 part, Mn0.03-0.06 part, Mo0.012-0.017 part, Zn0.04-0.07 part, and chelate 1.1-1.5 parts. Ensuring the nutrient elements needed by the normal growth of the plants.
the total nitrogen content of the water-soluble fertilizer is 12-15%, the phosphorus pentoxide content is 11-14%, and the potassium oxide content is 12-14%; the total nitrogen contains 5.3-5.8% of nitrate nitrogen and 8.1-8.5% of ammonium nitrogen. The mother liquor is prepared according to the formula before spraying, and then the working nutrient solution is prepared.
The invention has the following beneficial effects:
1. The female parent of the sealwort for tissue culture is introduced, the quantity is small, the growth is slow, only one tuber is grown in the growth period every year, and the growth period can be prolonged and the tuber is enlarged by the existing transplanting method. Compared with the conventional sealwort, the method has the advantages of fast rooting, large tuber (formerly 0.5CM, now more than or equal to 1.0 CM), high survival rate (formerly 78 percent, now more than or equal to 92 percent) through selective transplanting of the transplanting substrate, short period (formerly 12 months, now less than or equal to 9 months), fast growth and short rooting time (formerly 35 days, now less than or equal to 25 days), and reaches the standard of factory seedling culture.
2. The seeds are sleeved with plastic bags one month before the seeds of the sealwort are mature so as to prevent the seeds from falling off in advance or being eaten by insects. The mature seeds are collected for tissue culture, so that the germination rate of the seeds is improved, and the seedling culture time is shortened. By increasing the survival rate of outdoor transplantation, more tubers are grown to provide vegetative tubers. After the tubers of the sealwort are picked back, the roots are removed, the tubers are cleaned, aired, irradiated by an ultraviolet lamp for 8 hours, sealed by a transparent plastic bag and refrigerated for 10-15 days.
Drawings
FIG. 1 is a diagram of outdoor transplantation status of rhizoma Polygonati tissue culture seedlings;
Wherein each seedling A takes root 8 pieces, and the length is about 3 cm;
b, transplanting the seedlings into 50-hole plug trays;
C, growing plug seedlings for 25-30 days;
The length of the D root system reaches more than 10cm, and the number of the leaves is about 4;
e, transplanting the seedlings into outdoor flowerpots for the second time;
F, after transplanting for 45-60 days, the tuber reaches 1-3 cm; transplanting the plug seedlings to the outdoor after 25-30 days, wherein the survival rate is more than 92%.
Detailed Description
The invention is further illustrated by the following examples, but the scope of the invention as claimed is not limited to the scope of the examples.
example 1
an outdoor transplanting method of sealwort tissue culture seedlings, which comprises the following steps:
s1: transplanting the substrate, transplanting the test-tube plantlet and pre-treating the plug tray:
S2: and (3) management in a transplanting period:
S3, transplanting the plug seedlings to the outdoor after 25-30 days;
And finishing the outdoor transplantation of the sealwort tissue culture seedlings.
Preferably, the transplanting matrix in the step S1 comprises the following components in parts by weight: 1.5 parts of peat, 5 parts of river sand, 3 parts of bran coat and 2 parts of cottonseed hull.
Preferably, the transplanting matrix processing method in step S1 is: the substrate was wetted with 0.1% aqueous potassium permanganate solution and covered with a plastic film for 24 hours. Before the substrate is proportioned, the cottonseed hulls can be used after being added with water and fermented at high temperature for 1 month; the bran coat with impurities removed is put into a white cloth bag for high-temperature sterilization, and then put into a plastic basin soaked by 0.1% potassium permanganate aqueous solution for natural cooling for standby. Removing impurities from river sand collected by a river edge by using a 12-hole iron sieve, then carrying out outdoor exposure for one week, turning over twice every day during exposure, and then subpackaging the river sand in a clean bag to be stored indoors for later use. And weighing and proportioning according to the number of the transplanted seedlings.
preferably, the specific method for sterilizing the transplanted test-tube plantlet in the step S1 is as follows: rinsing the culture medium on the seedlings in water, soaking the seedlings in 2% sodium hypochlorite for 8 minutes, then soaking the seedlings in 50% ABT aqueous solution for 13 minutes, fishing out the seedlings, and sucking water by using sterile paper for transplanting.
Preferably, the step S1 method for managing the transplanting period includes: soaking the hole trays with 1000 times of 0.1% potassium permanganate aqueous solution for 24 hours before transplanting, then improving the nano-film technology to be applied to transplanting, automatically controlling the humidity to be more than 82%, controlling the temperature to be within the range of 5-25 ℃, controlling the illumination intensity to be 1800-2500 Lx, carrying out outdoor transplanting on the 28 th day, selecting a rainy day during transplanting, placing under a tree shade or a shady net for seedling revival after transplanting, and gradually adapting to the outdoor environment. Immediately spraying 1000 times carbendazim solution on the hole tray after transplanting, arching by using a plastic film, controlling the humidity to be more than 80% and the temperature to be within 20 ℃, controlling the illumination intensity to be 2200Lx in the daytime, and removing the plastic film on the 28 th day.
Preferably, in the step S2, the disease control in the transplanting stage management: observing the growth condition and the surrounding environment of the plant every day after transplanting, brushing the plant with phosphorus-containing washing powder water or brushing the plant with water soaked by cigarette ends for several times when aphids are found until no aphids appear, mainly preventing the aphids, controlling humidity, cleaning the plant with the diseases when the diseases occur, deeply turning over soil for insolation, and applying potash fertilizer to improve the resistance; when snails are found, spraying Wolk star on the peripheral water source area can prevent the snails. Fertilizing in the transplanting period management of the step S2: when the soybeans are transplanted, 10-15 cooked soybeans are placed at the bottom of the pot, the cooked soybeans are watered after 25 days, meanwhile, 20-8-28TE water-soluble fertilizers (high-nitrogen potassium type major element water-soluble fertilizers 20-8-28+ TE-Gadola) are sprayed in a cross mode, and nutrient elements needed by normal growth of plants are guaranteed.
Preferably, the foliar fertilizer comprises the following components in parts by weight: p2O510 parts of + K2o20 parts
+ Mg0.15 parts, 0.19 parts, Cu0.05 parts, Fe0.10 parts, Mn0.05 parts, Mo0.015 parts, Zn0.05 parts and chelate 1.24 parts.
The total nitrogen content of the nitrogen phosphorus potassium fertilizer is 14 percent, the phosphorus pentoxide content is 13 percent, and the potassium oxide content is 13 percent; the content of nitrate nitrogen in the total nitrogen is 5.6 percent, and the content of ammonium nitrogen is 8.4 percent. The mother liquor is prepared according to the formula before spraying, and then the working nutrient solution is prepared.
The method for obtaining the sealwort seedlings comprises the following steps:
1. Rooting culture of the regeneration plant: cutting adventitious buds with tender green leaves, vigorous growth and 3-4 leaves, inserting into MS minimal medium with ABT 0.3-0.5mg/L and AC 0.2-0.5g/L for rooting, adding sucrose 10-20g/L, agar 6.0g/L, pH 5.8-6.0, and sterilizing at 121 deg.C for 30 min;
2. Hardening and transplanting seedlings: when the height of the rooted tissue culture seedling is more than 5cm and the root length is more than 2cm, moving the tissue culture seedling to a seedling hardening room to adapt to the environment, then transplanting after continuously 15 days by using electromagnetic wave radiation frequency of 50-80MHz for 8h every day; and meanwhile, carrying out temperature change treatment, controlling the minimum temperature to be 8 ℃ and the maximum temperature to be 25 ℃ for 8h every day for 15-30 days continuously, and then transplanting, so that the survival rate and the germination rate can be improved.
The plants are transplanted in a divided manner in spring every year, and daily management is carried out: watering, fertilizing, weeding, pest control and the like.
example 2
The growth conditions were as shown in Table 1, except that the substrate was changed as in example 1.
TABLE 1 rhizoma Polygonati transplantation growth conditions of different substrates
from table 1, peat: river sand: bran coat: 1.5 parts of cottonseed hulls: 5: 3: 2, the best seeding substrate has the tuber growth time of 45d, the first tuber length of 1cm and the plant survival rate of 92 percent, and the growth time and the survival rate are greatly increased.
Table 2 example 1 comparison with conventional treatment
as can be seen from Table 2, the invention and the conventional treatment optimization show that the rooting time is 10 days earlier than the conventional time, the tuber of the invention is 0.5cm larger than the conventional treatment in the same period, the survival rate of the invention is 14 percent higher than the conventional treatment, and the purpose of industrialization is achieved.
example 3
On the basis of example 1, the transplanting matrix in the step S1 comprises the following components in parts by weight: 1.5 parts of peat, 5 parts of river sand, 3 parts of bran coat, 2 parts of cottonseed hull and 1.5 parts of Maillard reaction product. The preparation method of the Maillard reaction product comprises the following steps: weighing the same amount of glucosamine and xylan, dissolving in 0.95-1.2% glacial acetic acid solution, adjusting pH to 5.8-6 with 1mol/L sodium hydroxide solution, and reacting at 90-130 deg.C for 25-35min to obtain Maillard reaction product. The growth conditions of the sealwort transplanted by the method of the embodiment in different substrates are shown in table 3.
TABLE 3 transplanting growth of Polygonatum sibiricum
as can be seen from Table 3, on the basis of the inorganic substrate of the present invention, a certain amount of chemical substrate, i.e., Maillard reaction product, is added, so that the transplanting survival rate can be improved, and simultaneously, the number of rooted roots and the length of root systems can be improved, in this embodiment, the goal of fully dissolving glucosamine and effectively controlling the advanced glycosylation end product is achieved by adopting pH5.8-6, and the obtained Maillard reaction product can be wrapped on the surface of microbial cells to hinder the metabolism thereof, so that the growth of microbial infectious microbes in the transplanting process can be effectively inhibited, and the deterioration and decay of tubers are prevented. In addition, because the amino sugar Maillard series reaction product has certain viscosity, presents a weak gel state, has better adhesiveness and film forming property to tubers, and can physically protect tubers from being damaged to a certain extent, and simultaneously because the Maillard series reaction product has strong reducibility, the degradation of protein and amino acid at damaged parts in the process of transplanting tubers can be effectively inhibited, the survival rate and the growth speed of the tubers are further improved, and the growth cycle is further shortened.
Claims (8)
1. An outdoor transplanting method of sealwort tissue culture seedlings is characterized by comprising the following steps:
S1: transplanting the substrate, transplanting the test-tube plantlet and pre-treating the plug tray:
S2: and (3) management in a transplanting period:
S3, transplanting the plug seedlings to the outdoor after 25-30 days;
And finishing the outdoor transplantation of the sealwort tissue culture seedlings.
2. The method as claimed in claim 1, wherein the transplanting matrix in the step S1 comprises the following components in parts by weight:
1.3 to 1.9 parts of peat, 4.5 to 5.5 parts of river sand, 2.5 to 3.5 parts of bran coat and 1.5 to 2.5 parts of cottonseed hull.
3. the method as claimed in claim 1, wherein the step S1 pre-treatment method for the transplanted substrate is as follows: the matrix is wetted by 0.1 percent potassium permanganate aqueous solution and covered with the plastic film for 22 to 26 hours; the matrix is arranged in a plastic square plate, placed under an ultraviolet lamp, irradiated for 7-9 h every day, and treated for 10-15 days for later use.
4. The method according to claim 1, wherein the step S1 of sterilizing the transplanted test-tube plantlet comprises: rinsing the culture medium on the seedlings in water, soaking the seedlings in 1.5-2.5% sodium hypochlorite for 5-10 minutes, then soaking the seedlings in 45-55% ABT aqueous solution for 10-15 minutes, taking out the seedlings, and sucking water with sterile paper for transplanting.
5. The method of claim 1, wherein the step S2 is a method for managing the transplanting period: soaking the hole tray with 1000 times of 0.1% potassium permanganate aqueous solution for 22-26 h before transplanting, controlling the humidity to be 65% -85%, the temperature to be 15-25 ℃, the illumination intensity to be 1800-2500 Lx, carrying out outdoor transplanting in 25-30 days, selecting rainy days during transplanting, putting under tree shade or shade net after transplanting for seedling revival, and gradually adapting to outdoor environment.
6. The method according to claim 1, wherein the step S2 is a method for controlling diseases in the management of transplanting stage: observing the growth condition and the surrounding environment of the plant every day after transplanting, brushing the plant with phosphorus-containing washing powder water or brushing the plant with water soaked by cigarette ends for several times when aphids are found until no aphids appear, mainly preventing the aphids, controlling humidity, cleaning the plant with the diseases when the diseases occur, deeply turning over soil for insolation, and applying potash fertilizer to improve the resistance; when snails are found, spraying Wolk star on the peripheral water source area can prevent the snails.
7. the method of claim 1, wherein the step S2 is applied in management of transplanting stage: and (3) placing 10-15 cooked soybeans at the bottom of the pot during transplanting, irrigating decomposed cake fertilizer water after 25 days, and simultaneously spraying water-soluble fertilizer and leaf fertilizer in a crossed manner.
8. The method as claimed in claim 7, wherein the foliar fertilizer comprises the following components in parts by weight:
P2O58-12 parts, K2o18-22 parts, Mg0.12-0.17 part, S0.15-0.22 part, Cu0.03-0.06 part, Fe0.05-0.15 part, Mn0.03-0.06 part, Mo0.012-0.017 part, Zn0.04-0.07 part, and chelate 1.1-1.5 parts.
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| CN111937703A (en) * | 2020-08-24 | 2020-11-17 | 三明市农业科学研究院 | Method for breeding high-quality polygonatum cyrtonema seedlings |
| CN115250873A (en) * | 2022-07-27 | 2022-11-01 | 中国长江三峡集团有限公司 | Transplanting matrix for anoectochilus roxburghii tissue culture seedlings, preparation method and transplanting method |
| CN115362897A (en) * | 2022-09-26 | 2022-11-22 | 西南林业大学 | Polygonatum sibiricum cultivation method capable of improving quality of polygonatum sibiricum |
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