CN114128578B - Facility seedling raising method for polygonatum cyrtonema seedling - Google Patents

Facility seedling raising method for polygonatum cyrtonema seedling Download PDF

Info

Publication number
CN114128578B
CN114128578B CN202111414072.7A CN202111414072A CN114128578B CN 114128578 B CN114128578 B CN 114128578B CN 202111414072 A CN202111414072 A CN 202111414072A CN 114128578 B CN114128578 B CN 114128578B
Authority
CN
China
Prior art keywords
seedling
days
seeds
polygonatum cyrtonema
management
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202111414072.7A
Other languages
Chinese (zh)
Other versions
CN114128578A (en
Inventor
韩量
杨天建
肖波
蒲盛才
余中莲
全健
雷美艳
李娜
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CHONGQING INSTITUTE OF MEDICINAL PLANT CULTIVATION
Original Assignee
CHONGQING INSTITUTE OF MEDICINAL PLANT CULTIVATION
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CHONGQING INSTITUTE OF MEDICINAL PLANT CULTIVATION filed Critical CHONGQING INSTITUTE OF MEDICINAL PLANT CULTIVATION
Priority to CN202111414072.7A priority Critical patent/CN114128578B/en
Publication of CN114128578A publication Critical patent/CN114128578A/en
Application granted granted Critical
Publication of CN114128578B publication Critical patent/CN114128578B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08—Immunising seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00—Soilless cultivation, e.g. hydroponics

Abstract

The invention provides a facility seedling raising method of polygonatum cyrtonema seedling, which mainly comprises seven steps: seed collection and cleaning, first seed disinfection, seed soaking, second seed disinfection, first-year seed germination management, second-year first-leaf seedling management and third-year strong seedling management. By adopting the seedling raising method provided by the invention, on one hand, the germination rate and the emergence rate of seeds are effectively improved, the sizes of primary rootstocks and seedling tubers are increased, and the root systems of the cultivated seedlings are more developed; on the other hand, the field management cost is reduced, the large-scale production of polygonatum cyrtonema seeds and seedlings is facilitated, and sufficient high-quality seed sources are provided for polygonatum cyrtonema planting.

Description

Facility seedling raising method for polygonatum cyrtonema seedling
Technical Field
The invention relates to the technical field of planting of traditional Chinese medicinal materials polygonatum cyrtonema, in particular to a facility seedling raising method of polygonatum cyrtonema seedlings.
Background
Polygonatum cyrtonema (Polygonatum cyrtonema Hua) is one of basic source plants of a traditional Chinese medicine, is a traditional bulk medicine and food dual-purpose medicine in China, and has the effects of tonifying qi and yin, strengthening spleen, moistening lung, tonifying kidney and the like. Is used for treating qi deficiency of spleen and stomach, tiredness, debilitation, stomach yin deficiency, dry mouth, anorexia, cough due to lung deficiency, cough due to fatigue, hemoptysis, deficiency of essence and blood, soreness of waist and knees, premature graying of hair, internal heat, and diabetes.
Polygonatum cyrtonema is a plant of the genus Polygonatum of the family Liliaceae, and is mainly distributed in the province of the province along the Yangtze river. The propagation mode of Polygonatum cyrtonema Fabricius mainly comprises three modes of rhizome propagation, seed propagation and tissue culture. The rootstock can be used as a propagation material and commodity at the same time, which not only leads to serious resource exhaustion, but also causes serious problems of seed degeneration, disease diffusion and the like in the asexual propagation of multiple generations, and directly leads to the reduction of commodity yield and quality. Meanwhile, the rhizome propagation method has low propagation coefficient, large seed consumption and high production cost, and seedling supply becomes a restriction bottleneck for large-scale cultivation. The tissue culture mode for producing the seedlings has the problems of high facility equipment requirement, complicated operation and high comprehensive cost, and is difficult to apply to large-scale production. Seed reproduction has the advantages of large seed quantity and relatively low cost. But has lower germination rate, long seedling time and low seedling rate under natural conditions. The following problems are mainly present: firstly, the polygonatum cyrtonema seeds have morphological after-ripening after harvesting, the seeds absorb water slowly and contain germination inhibiting substances, and the like, have long dormancy period, have low germination rate in the first year and are easy to rot in the process; secondly, the seedling raising time is long, and the management difficulty is high. Under natural conditions, the Polygonatum cyrtonema Fabricius can grow 1 true leaf after being dormant in 2 winter, one leaf period lasts for one year, overground parts can grow to more than ten centimeters after three years, and the Polygonatum cyrtonema Fabricius can become high-quality seedlings in the fourth year. In the process of field seed seedling raising, too many leaf diseases such as seedling gray mold, leaf spot, anthracnose and the like and root rot are generated in spring, summer and rainy seasons, so that the growth of seedlings is seriously affected; the field weeds are luxuriant, and the management difficulty of weeding, water and fertilizer, diseases and the like is extremely high; thirdly, root hairs are easy to be damaged during seedling transplanting, and the subsequent growth quantity is affected.
Therefore, the seedling raising mode of the polygonatum cyrtonema seeds is adjusted by combining modern agricultural facilities, the greenhouse facilities are adopted for raising seedlings, water, fertilizer and weeds in the seedling raising environment are controlled, the disease incidence rate of the polygonatum cyrtonema can be effectively reduced, the seedling growth quality is improved, the high-quality strong seedlings of the polygonatum cyrtonema are produced, the technical problem of excellent polygonatum cyrtonema seedling production is practically solved, the large-scale and standardized seedling raising of the polygonatum cyrtonema is realized, and the development of the polygonatum cyrtonema industry is promoted.
Disclosure of Invention
In order to solve the technical problems, the invention provides a facility seedling raising method for polygonatum cyrtonema seedling, which adopts repeated disinfection and seedling differentiation management in different periods, effectively improves seed germination rate and emergence rate, increases seedling tuber weight and improves seedling quality.
The technical scheme of the invention is as follows:
the invention provides a facility seedling raising method of polygonatum cyrtonema seedlings, which comprises the following steps:
s1, seed collection and cleaning: after the color of the polygonatum cyrtonema fruits is changed from green to black, collecting and filling the polygonatum cyrtonema fruits into a breathable woven bag; when the fruits are softened, the fruits are kneaded and rinsed to remove pulp and peel, and clean seeds are obtained;
s2, first disinfection of seeds: soaking the seeds treated in the step S1 in a disinfectant I for 1-2 hours, and draining excessive water;
s3, soaking seeds: soaking the seeds treated in the step S2 in clear water for 2-3 hours every day at 15-25 ℃, draining excessive water, and continuously operating for 10-20 days to ensure that the seeds are saturated with water until 3-6% of the seeds germinate;
s4, secondary disinfection of seeds: coating the seeds treated in the step S3 with a sterilizing seed coating agent for later use;
s5, managing germination period of the seeds in the first year: sowing the seeds treated in the step S4 in a foam box paved with 3-5CM storage matrixes, covering 1-2CM storage matrixes, covering the foam box, controlling the ambient temperature to 15-20 ℃ in the middle 3 months of the first year, keeping the matrix moisture to be 60-70% so as to promote the germination of primary roots and stems, managing under natural temperature conditions after the formation of the primary roots and stems, keeping the humidity unchanged, spraying trichoderma harzianum and potassium fulvate every 1 month after sowing, and promoting the growth of root systems and the expansion of the primary roots and stems;
s6, seedling management in the first leaf stage of the second year: after the primary rhizome enters a dormancy period, carrying out disinfection treatment for standby, then transferring the primary rhizome into a cave dish I or a flat dish which is fully paved with seedling substrates, covering 1-2CM of the surface of the cave dish I or the flat dish with the seedling substrates, placing the cave dish I or the flat dish in a greenhouse, keeping the humidity of the substrates at 40-70%, performing nutrient management and pest and disease management, and controlling the shading degree at 65-75%, wherein the cave dish I is 200 holes;
s7, strong seedling management in the third year: sterilizing rhizoma Polygonati in one leaf stage after dormancy stage, transferring into tray II or flat plate full of seedling substrate, slightly pressing, covering 1-2cm seedling substrate, placing tray II or flat plate in greenhouse, maintaining moisture content of substrate at 40-70%, performing nutrient management and pest and disease control, and controlling shading degree at 45-55%，The plug II is 50 holes.
In the method, the disinfectant I in the step S2 is a carbendazim solution with the concentration of 0.2-0.3%.
In the method, the bactericidal seed coating agent in S4 is 62.5g/L of fine methyl fludioxonil or 4.8% of phenyl ether fludioxonil or 11% of fine methyl fludioxonil, the 62.5g/L of fine methyl fludioxonil and seeds are mixed according to the ratio of 1:250-333, the 4.8% of phenyl ether fludioxonil and the seeds are mixed according to the ratio of 1:350-500, and the 11% of fine methyl fludioxonil and 100 kg of seeds are mixed according to the ratio of 200-400 ml/100 kg of seeds, wherein the percentage by mass is.
In the method, the storage substrate in the step S5 is peat, perlite is uniformly mixed according to the proportion of 2-2.5:1, and soil disinfectant is used for disinfection for standby.
In the above method, the process of S5 is completed indoors or in a greenhouse.
In the method, the seedling substrate S6 is peat, coconut coir and perlite which are uniformly mixed according to the proportion of 3-4:2:1, and the mixture is sterilized by using a soil disinfectant for standby.
In the method, the nutrient management in S6 is that trichoderma harzianum and potassium fulvate are sprayed once every 20-30 days before emergence of seedlings for 60-70 days, so that root system growth is promoted, and KH is sprayed on leaf surfaces every 15-20 days at the same time 2 PO 4 The nutrient is supplemented once, the seedling emergence is carried out for 70 days to the seedling pouring, and the N: P: K balanced water-soluble fertilizer is applied once every 20 days.
In the method, S6, the pest and disease control is started when the emergence rate reaches 50%, and carbendazim and mancozeb are used alternately every 15 days to prevent diseases; when the foliar diseases occur, amitraz (azoxystrobin) is used for the treatment by compounding pyrimethanil or procymidone or penthiopyrad or picoxystrobin; when the rhizome disease occurs, hymexazol, thifluzamide or fluxapyroxad is used for treatment; the pest in the greenhouse can be prevented and controlled in a physical mode when the pest is less.
In the method, S7, when the temperature of the greenhouse is above 15 ℃, spraying Trichoderma harzianum and potassium fulvate once every 20 days to promote root growth for 2-3 times; spraying KH on the leaf surface 30 days before emergence 2 PO 4 Supplement nutrients, each timeSpraying once every 15 days; the seedling emergence lasts for 30 to 90 days, and the high-nitrogen type macroelement water-soluble fertilizer is applied once every 20 days, so that the growth of the leaves is promoted; and the seedlings are planted for 90 days to the seedling pouring, and the high-potassium type macroelement water-soluble fertilizer is applied once every 20 days to promote the rhizome expansion.
In the method, the pest and disease control of S7 is started when the emergence rate reaches 50%, and carbendazim and mancozeb are alternately applied every 15 days to prevent diseases; when the foliar diseases occur, amitraz (azoxystrobin) is used for the treatment by compounding pyrimethanil or procymidone or penthiopyrad or picoxystrobin; when the rhizome disease occurs, hymexazol, thifluzamide or fluxapyroxad is used for treatment; the pest in the greenhouse can be prevented and controlled in a physical mode when the pest is less.
Compared with the prior art, the invention has the following beneficial effects:
1. the polygonatum cyrtonema seeds adopt the treatment modes of repeated disinfection, continuous seed soaking, indoor matrix temperature control, humidity control, germination, spraying microbial inoculum, fertilizer and the like, and the rotting rate of the seeds is reduced to below 5 percent; the germination rate of seeds reaches more than 92%; the weight of the nascent rhizome can reach 0.12-0.15g, and the number of root systems is 5-10.
2. Dynamic management is adopted in different growth periods, so that the occurrence of diseases can be effectively controlled, the shading degree, the matrix water content and the nutrient supply in the growth environment are accurately controlled, and the seedling rate in the biennial one-leaf period is up to more than 98%; the fresh weight of tubers is 0.5-1.2g, and the fresh weight of tubers can be more than 2g in a small amount; the root system is developed, and the number is 10-25. The seedling rate of three-year seedlings reaches more than 97 percent; the fresh weight of the tuber with root is 6-10g, the weight of a few parts can reach more than 15g, the number of buds is 2-3, and the tuber length is 3-6cm; the root system is developed, and the number is more than 25.
3. The matrix is adopted for carrying out concentrated seedling culture on the polygonatum cyrtonema seedling, so that the damage of external weed seeds and insect pests are reduced, the field management cost is reduced, the large-scale production of the polygonatum cyrtonema seedling is facilitated, and sufficient high-quality seed sources are provided for the polygonatum cyrtonema planting.
4. Under the condition of ensuring good seedling growth, the porous plug seedling is adopted as much as possible by adjusting the nutrient supply mode, so that the space can be effectively utilized, and the management cost is reduced. 50 ten thousand high-quality biennial polygonatum cyrtonema seedlings and 15 ten thousand high-quality three-year-old polygonatum cyrtonema seedlings can be provided for each mu of greenhouse. And the plug seedling effectively avoids the mutual winding of root systems, can achieve rapidness, carry soil and not hurt the root when the seedling is transplanted, can quickly recover the growth after the seedling is transplanted, and greatly improves the survival rate of the seedling and the growth quantity in the current year.
Drawings
FIG. 1 shows growth of a biennial Polygonatum cyrtonema of example 1;
FIG. 2 shows growth of three-year-old Polygonatum cyrtonema Fabricius of example 1;
FIG. 3 shows growth of the biennial Polygonatum cyrtonema of example 2;
FIG. 4 shows growth of three-year-old Polygonatum cyrtonema Fabricius of example 2;
FIG. 5 shows growth of the biennial Polygonatum cyrtonema of example 3;
FIG. 6 shows growth of three-year-old Polygonatum cyrtonema Fabricius of example 3.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Example 1
The embodiment provides a facility seedling raising method of polygonatum cyrtonema seedlings, which comprises the following steps:
s1, seed collection and cleaning: after the color of the polygonatum cyrtonema fruits is changed from green to black, collecting and filling the polygonatum cyrtonema fruits into a breathable woven bag; and (5) after the fruits become soft, rubbing and rinsing to remove pulp and peel, and obtaining clean seeds.
S2, first disinfection of seeds: soaking the seeds treated in the step S1 with carbendazim with the concentration of 0.2% for 2 hours, and draining excessive water.
S3, soaking seeds: soaking the seeds treated in the step S2 in clean water for 2 hours every day at the temperature of 25 ℃, draining excessive water, and continuously operating for 20 days to ensure that the seeds are saturated with water until 6% of the seeds germinate.
S4, secondary disinfection of seeds: and (3) dressing the seeds treated by the S3 with 62.5g/L of fludioxonil according to the medicine-seed ratio of 1:250-333 for later use.
S5, managing germination period of the seeds in the first year: and at the beginning of 12 months, sowing the seeds treated by the step S4 into a foam box paved with a 5CM storage matrix, covering the foam box with the 1CM storage matrix, and controlling the ambient temperature at 20 ℃ and keeping the matrix moisture within the range of 60-70% so as to promote seed germination. And at the beginning of 3 months of the next year, after the primary rootstock is formed, the management is carried out under the natural temperature condition, and the humidity is kept unchanged. And 4, spraying trichoderma harzianum and potassium fulvate once every 1 month from the beginning of sowing to the beginning of the period of dormancy of the birth rootstock, and promoting the growth of root systems and the expansion of the primary rootstock.
S6, seedling management in the first leaf stage of the second year: and (3) sterilizing the nascent rhizome in a dormancy stage by using a carbendazim solution with the concentration of 0.1% for 30 minutes, taking out, and slightly airing for later use. Spreading seedling substrate in 200 holes of plug tray, slightly pressing, putting 1 primary rhizome into each hole, covering 1CM seedling substrate on surface, and placing plug tray in greenhouse. The water management is as follows: when Polygonatum cyrtonema Fabricius does not emerge in winter, water is sprayed for 3-5 minutes every three days, and the water content of the seedling substrate is kept at 40-60% humidity; the temperature in the greenhouse is above 20 ℃ in spring, a piece of true leaves grow out of Polygonatum cyrtonema Fabricius seeds successively, water is sprayed for 3-5 minutes every two days, and the moisture content of the seedling substrate is kept at 50-70% humidity; the temperature in the greenhouse in summer is above 30 ℃, water is sprayed for 3-5 minutes each day, and the moisture content of the seedling substrate is kept at 50-70% humidity; after the polygonatum cyrtonema is fallen in autumn, water is sprayed for 3-5 minutes every three days, and the water content of the seedling substrate is kept at 40-60% of humidity. The nutrient management is as follows: 70 days before emergence, the root system of the polygonatum cyrtonema seedling is undeveloped, trichoderma harzianum and potassium fulvate are sprayed once every 20 days, and the root system growth is promoted; simultaneously spraying KH on leaf surfaces 2 PO 4 Supplementing nutrients, and spraying once every 15 days; and (3) after 70 days of seedling emergence to seedling pouring, the macroelement water-soluble fertilizer is applied once every 20 days in a proportion of N to P to K of 21:21:21. The pest management is as follows: the emergence rate is 50%, and the carbendazim and mancozeb are alternately applied every 15 days to prevent diseases; when foliar diseases occur, amikacin (azoxystrobin) is used for compounding pyrimethanil and procymidoneTreatment is carried out on the penthiopyrad, picoxystrobin and picoxystrobin; when the rhizome diseases occur, hymexazol, thifluzamide and fluxapyroxad are used for treatment; and when insect pests in the greenhouse occur less, the insect pests are prevented and controlled by a physical method. The shading management is as follows: shading is performed by using 8-needle sunshade net, and the shading degree is controlled to be 70%.
S7, seedling management in the third year: soaking rhizoma Polygonati in 0.1% carbendazim solution for 30 min after entering dormancy stage, taking out, and air drying. Spreading seedling substrate in 50-hole tray, slightly pressing, placing 1 seedling in one leaf stage in each hole, covering 1cm of the substrate, and placing the tray in greenhouse. The water management is as follows: when Polygonatum cyrtonema Fabricius does not emerge in winter, water is sprayed for 3-5 minutes every three days, and the water content of the seedling substrate is kept at 40-60% humidity; the temperature in the greenhouse is above 20 ℃ in spring, the Polygonatum cyrtonema seeds start to emerge successively, water is sprayed for 4-6 minutes every two days, and the water content of the seedling substrate is kept at 50-70% humidity; the temperature in the greenhouse in summer is above 30 ℃, water is sprayed for 4-6 minutes each day, and the moisture content of the seedling substrate is kept at 50-70% humidity; after the polygonatum cyrtonema is fallen in autumn, water is sprayed for 4-6 minutes every three days, and the water content of the seedling substrate is kept at 40-60% of humidity. The nutrient management is as follows: spraying Trichoderma harzianum and potassium fulvate once every 20 days at the temperature of the greenhouse above 15 ℃ to promote root growth for 2-3 times, spraying KH on leaf surfaces 30 days before emergence 2 PO 4 Supplementing nutrients, and spraying once every 15 days; the seedling emergence lasts for 30 to 90 days, and the high-nitrogen macroelement water-soluble fertilizer with the ratio of N to P to K of 30:10:10 is applied every 20 days, so that the growth of the leaves is promoted; and the seedlings are planted for 90 days to the seedling pouring, and the high-potassium type macroelement water-soluble fertilizer with the ratio of N to P to K of 14 to 30 is applied every 20 days, so that the rhizome expansion is promoted. The pest management is as follows: the emergence rate is 50%, and the carbendazim and mancozeb are alternately applied every 15 days to prevent diseases; when the foliar diseases occur, amitraz (azoxystrobin) is used for the treatment of the foliar diseases by compounding with pyrimethanil, procymidone, penthiopyrad and picoxystrobin; when the rhizome diseases occur, hymexazol, thifluzamide and fluxapyroxad are used for treatment; and when insect pests in the greenhouse occur less, the insect pests are prevented and controlled by a physical method. The shading management is as follows: sunshade using 6 needlesThe mesh was shaded with the shading degree controlled at 50%.
Wherein the storage matrix is peat and perlite are uniformly mixed according to the proportion of 2:1, and the peat and perlite are sterilized by using a soil disinfectant for later use; the seedling substrate is peat, coconut coir and perlite which are uniformly mixed according to the proportion of 3:2:1, and the mixture is sterilized by using a soil disinfectant for standby.
In this embodiment, the growth condition of the Polygonatum cyrtonema in the biennial one-leaf period is shown in fig. 1; the growth condition of the three-year-old polygonatum cyrtonema seedling is shown in figure 2.
Example 2
The embodiment provides a facility seedling raising method of polygonatum cyrtonema seedlings, which comprises the following steps:
s1, seed collection and cleaning: after the color of the polygonatum cyrtonema fruits is changed from green to black, collecting and filling the polygonatum cyrtonema fruits into a breathable woven bag; and (5) after the fruits become soft, rubbing and rinsing to remove pulp and peel, and obtaining clean seeds.
S2, first disinfection of seeds: soaking the seeds treated in the step S1 with carbendazim with the concentration of 0.3% for 1 hour, and draining excessive water.
S3, soaking seeds: soaking the seeds treated in the step S2 in clean water for 3 hours every day at 15 ℃, draining excessive water, and continuously operating for 10 days to ensure that the seeds are saturated with water until 3% of the seeds germinate.
S4, secondary disinfection of seeds: and (3) mixing the seeds treated by the S3 with 4.8% of phenylate fludioxonil by mass percent according to a medicine-seed ratio of 1:350-500 for later use.
S5, managing germination period of the seeds in the first year: and (3) sowing the seeds treated by the step (S4) in a foam box paved with a 3CM storage matrix, covering the foam box with a 2CM storage matrix, and controlling the ambient temperature at 15 ℃ and keeping the matrix moisture within the range of 60-70% so as to promote seed germination. And at the beginning of 3 months of the next year, after the primary roots are formed, the management is carried out under the condition of natural temperature, the humidity is kept unchanged, and trichoderma harzianum and potassium fulvate are sprayed once every 1 month after sowing, so that the root growth and the primary roots expansion are promoted.
S6, seedling management in the first leaf stage of the second year: the primary rhizome enters dormancyAfter the period, sterilizing with 0.1% carbendazim solution for 30 min, taking out, and air drying slightly for use. The seedling substrate is fully paved in a perforated flat tray, the seedling substrate is slightly pressed, the nascent rhizome is put in, the surface of the seedling substrate is covered with 2CM, and the tape Kong Pingpan is placed in a greenhouse. The water management is as follows: when Polygonatum cyrtonema Fabricius does not emerge in winter, water is sprayed for 3-5 minutes every three days, and the water content of the seedling substrate is kept at 40-60% humidity; the temperature in the greenhouse is above 20 ℃ in spring, a piece of true leaves grow out of Polygonatum cyrtonema Fabricius seeds successively, water is sprayed for 3-5 minutes every two days, and the moisture content of the seedling substrate is kept at 50-70% humidity; the temperature in the greenhouse in summer is above 30 ℃, water is sprayed for 3-5 minutes each day, and the moisture content of the seedling substrate is kept at 50-70% humidity; after the polygonatum cyrtonema is fallen in autumn, water is sprayed for 3-5 minutes every three days, and the water content of the seedling substrate is kept at 40-60% of humidity. The nutrient management is as follows: 70 days before emergence, the root system of the polygonatum cyrtonema seedling is undeveloped, trichoderma harzianum and potassium fulvate are sprayed once every 20 days, and the root system growth is promoted; simultaneously spraying KH on leaf surfaces 2 PO 4 Supplementing nutrients, and spraying once every 15 days; and (3) after 70 days of seedling emergence to seedling pouring, the macroelement water-soluble fertilizer is applied once every 20 days in a proportion of N to P to K of 21:21:21. The pest management is as follows: the emergence rate is 50%, and the carbendazim and mancozeb are alternately applied every 15 days to prevent diseases; when the foliar diseases occur, amitraz (azoxystrobin) is used for the treatment of the foliar diseases by compounding with pyrimethanil, procymidone, penthiopyrad and picoxystrobin; when the rhizome diseases occur, hymexazol, thifluzamide and fluxapyroxad are used for treatment; and when insect pests in the greenhouse occur less, the insect pests are prevented and controlled by a physical method. The shading management is as follows: shading is performed by using 8-needle sunshade net, and the shading degree is controlled to be 70%.
S7, seedling management in the third year: soaking rhizoma Polygonati in 0.1% carbendazim solution for 30 min after entering dormancy stage, taking out, and air drying. Spreading the seedling substrate in a perforated flat tray, slightly pressing, placing seedling in a leaf stage, covering the surface with the substrate for 2cm, and placing the tape Kong Pingpan on a stand in a greenhouse. The water management is as follows: when Polygonatum cyrtonema Fabricius does not emerge in winter, water is sprayed for 3-5 minutes every three days, and the water content of the seedling substrate is kept at 40-60% humidity; the temperature in the greenhouse is 20 in springAt above DEG C, the Polygonatum cyrtonema seeds start to emerge successively, water is sprayed for 4-6 minutes every two days, and the water content of the seedling substrate is kept at 50-70% humidity; the temperature in the greenhouse in summer is above 30 ℃, water is sprayed for 4-6 minutes each day, and the moisture content of the seedling substrate is kept at 50-70% humidity; after the polygonatum cyrtonema is fallen in autumn, water is sprayed for 4-6 minutes every three days, and the water content of the seedling substrate is kept at 40-60% of humidity. The nutrient management is as follows: spraying Trichoderma harzianum and potassium fulvate once every 20 days at the temperature of the greenhouse above 15 ℃ to promote root growth for 2-3 times, spraying KH on leaf surfaces 30 days before emergence 2 PO 4 Supplementing nutrients, and spraying once every 15 days; the seedling emergence lasts for 30 to 90 days, and the high-nitrogen macroelement water-soluble fertilizer with the ratio of N to P to K of 30:10:10 is applied every 20 days, so that the growth of the leaves is promoted; and the seedlings are planted for 90 days to the seedling pouring, and the high-potassium type macroelement water-soluble fertilizer with the ratio of N to P to K of 14 to 30 is applied every 20 days, so that the rhizome expansion is promoted. The pest management is as follows: the emergence rate is 50%, and the carbendazim and mancozeb are alternately applied every 15 days to prevent diseases; when the foliar diseases occur, amitraz (azoxystrobin) is used for the treatment of the foliar diseases by compounding with pyrimethanil, procymidone, penthiopyrad and picoxystrobin; when the rhizome diseases occur, hymexazol, thifluzamide and fluxapyroxad are used for treatment; and when insect pests in the greenhouse occur less, the insect pests are prevented and controlled by a physical method. The shading management is as follows: shading is performed by using a 6-needle sunshade net, and the shading degree is controlled to be 50%.
The storage matrix is peat, perlite is uniformly mixed according to the proportion of 2.5:1, and soil disinfectant is used for disinfection for later use; the seedling substrate is peat, coconut coir and perlite which are uniformly mixed according to the proportion of 4:2:1, and the mixture is sterilized by using a soil disinfectant for standby.
In this embodiment, the growth condition of the Polygonatum cyrtonema in the biennial one-leaf period is shown in FIG. 3; the growth condition of the three-year-old polygonatum cyrtonema seedling is shown in figure 4.
Example 3
The embodiment provides a facility seedling raising method of polygonatum cyrtonema seedlings, which comprises the following steps:
s1, seed collection and cleaning: after the color of the polygonatum cyrtonema fruits is changed from green to black, collecting and filling the polygonatum cyrtonema fruits into a breathable woven bag; and (5) after the fruits become soft, rubbing and rinsing to remove pulp and peel, and obtaining clean seeds.
S2, first disinfection of seeds: soaking the seeds treated in the step S1 with carbendazim with the concentration of 0.25% for 2 hours, and draining excessive water.
S3, soaking seeds: soaking the seeds treated in the step S2 in clear water for 2.5 hours every day at 20 ℃, draining excessive water, and continuously operating for 15 days to ensure that the seeds are saturated with water until 5% of the seeds germinate.
S4, secondary disinfection of seeds: mixing seeds treated by S3 with 11% by mass of America fludioxonil according to 200-400 ml/100 kg for later use.
S5, managing germination period of the seeds in the first year: and at the beginning of 12 months, sowing the seeds treated by the step S4 into a foam box paved with a 4CM storage matrix, covering the foam box with the 1CM storage matrix, and controlling the ambient temperature at 20 ℃ and keeping the matrix moisture within the range of 60-70% so as to promote seed germination. And at the beginning of 3 months of the next year, after the primary roots are formed, the management is carried out under the condition of natural temperature, the humidity is kept unchanged, and trichoderma harzianum and potassium fulvate are sprayed once every 1 month after sowing, so that the root growth and the primary roots expansion are promoted.
S6, seedling management in the first leaf stage of the second year: and (3) sterilizing the nascent rhizome in a dormancy stage by using a carbendazim solution with the concentration of 0.1% for 30 minutes, taking out, and slightly airing for later use. Spreading seedling substrate in flat trays, slightly pressing, placing 200 primary rootstocks in each tray, covering 1CM seedling substrate on the surface, and placing the flat trays in a greenhouse. The water management is as follows: when Polygonatum cyrtonema Fabricius does not emerge in winter, water is sprayed for 3-5 minutes every three days, and the water content of the seedling substrate is kept at 40-60% humidity; the temperature in the greenhouse is above 20 ℃ in spring, a piece of true leaves grow out of Polygonatum cyrtonema Fabricius seeds successively, water is sprayed for 3-5 minutes every two days, and the moisture content of the seedling substrate is kept at 50-70% humidity; the temperature in the greenhouse in summer is above 30 ℃, water is sprayed for 3-5 minutes each day, and the moisture content of the seedling substrate is kept at 50-70% humidity; after the polygonatum cyrtonema is fallen in autumn, water is sprayed for 3-5 minutes every three days, and the water content of the seedling substrate is kept at 40-60% of humidity. The nutrient management is as follows: 70 days before emergence of seedlings, the root system of Polygonatum cyrtonema Falcatum seedlings is undevelopedSpraying Trichoderma harzianum and potassium fulvate once every 20 days to promote root growth; simultaneously spraying KH on leaf surfaces 2 PO 4 Supplementing nutrients, and spraying once every 15 days; and (3) after 70 days of seedling emergence to seedling pouring, the macroelement water-soluble fertilizer is applied once every 20 days in a proportion of N to P to K of 21:21:21. The pest management is as follows: the emergence rate is 50%, and the carbendazim and mancozeb are alternately applied every 15 days to prevent diseases; when the foliar diseases occur, amitraz (azoxystrobin) is used for the treatment of the foliar diseases by compounding with pyrimethanil, procymidone, penthiopyrad and picoxystrobin; when the rhizome diseases occur, hymexazol, thifluzamide and fluxapyroxad are used for treatment; and when insect pests in the greenhouse occur less, the insect pests are prevented and controlled by a physical method. The shading management is as follows: shading is performed by using 8-needle sunshade net, and the shading degree is controlled to be 70%.
S7, seedling management in the third year: soaking rhizoma Polygonati in 0.1% carbendazim solution for 30 min after entering dormancy stage, taking out, and air drying. Spreading the seedling substrate in flat trays, slightly pressing, putting 50 seedlings in one leaf stage into each tray, covering the surface with the culture substrate for 1cm, and placing the flat trays in a greenhouse. The water management is as follows: when Polygonatum cyrtonema Fabricius does not emerge in winter, water is sprayed for 3-5 minutes every three days, and the water content of the seedling substrate is kept at 40-60% humidity; the temperature in the greenhouse is above 20 ℃ in spring, the Polygonatum cyrtonema seeds start to emerge successively, water is sprayed for 4-6 minutes every two days, and the water content of the seedling substrate is kept at 50-70% humidity; the temperature in the greenhouse in summer is above 30 ℃, water is sprayed for 4-6 minutes each day, and the moisture content of the seedling substrate is kept at 50-70% humidity; after the polygonatum cyrtonema is fallen in autumn, water is sprayed for 4-6 minutes every three days, and the water content of the seedling substrate is kept at 40-60% of humidity. The nutrient management is as follows: spraying Trichoderma harzianum and potassium fulvate once every 20 days when the temperature of the greenhouse is above 15 ℃, promoting root growth for 2-3 times in total, spraying KH2PO4 on leaf surfaces 30 days before emergence to supplement nutrients, and spraying once every 15 days; the seedling emergence lasts for 30 to 90 days, and the high-nitrogen macroelement water-soluble fertilizer with the ratio of N to P to K of 30:10:10 is applied every 20 days, so that the growth of the leaves is promoted; and the seedlings are planted for 90 days to the seedling pouring, and the high-potassium type macroelement water-soluble fertilizer with the ratio of N to P to K of 14 to 30 is applied every 20 days, so that the rhizome expansion is promoted. The pest management is as follows: the emergence rate is 50%, and the carbendazim and mancozeb are alternately applied every 15 days to prevent diseases; when the foliar diseases occur, amitraz (azoxystrobin) is used for the treatment of the foliar diseases by compounding with pyrimethanil, procymidone, penthiopyrad and picoxystrobin; when the rhizome diseases occur, hymexazol, thifluzamide and fluxapyroxad are used for treatment; and when insect pests in the greenhouse occur less, the insect pests are prevented and controlled by a physical method. The shading management is as follows: shading is performed by using a 6-needle sunshade net, and the shading degree is controlled to be 50%.
Wherein the storage matrix is peat and perlite are uniformly mixed according to the proportion of 2:1, and the peat and perlite are sterilized by using a soil disinfectant for later use; the seedling substrate is peat, coconut coir and perlite which are uniformly mixed according to the proportion of 3:2:1, and the mixture is sterilized by using a soil disinfectant for standby.
In this embodiment, the growth condition of the Polygonatum cyrtonema in the biennial one-leaf period is shown in FIG. 5; the growth condition of the three-year-old polygonatum cyrtonema seedling is shown in figure 6.
Comparative example 1
This comparative example provides a facility seedling method of Polygonatum cyrtonema Fabricius seedlings, which is different from example 1 in that:
and (3) the seeds treated in the step (S3) are not disinfected for the second time and are directly reserved.
Other steps were consistent with example 1.
The seed rot rate and germination rate comparison data are as follows, using the seedling raising methods of comparative example 1 and example 1, respectively:
table 1 comparative data on seed rot rate and germination rate in comparative example 1 and example 1
Figure SMS_1
As shown in Table 1, the use of the bactericidal seed coating agent for Polygonatum cyrtonema seed can greatly reduce the rotting rate of the seed and improve the germination rate of the seed.
Comparative example 2
This comparative example provides a facility seedling method of Polygonatum cyrtonema Fabricius seedlings, which is different from example 1 in that:
s5, managing germination period of the seeds in the first year: the germination was performed under natural temperature conditions without temperature control during the first 12 months to the second 3 months of the seed germination period, and the other conditions were the same as in example 1.
Comparative data of germination rates of Polygonatum cyrtonema seeds using the seedling methods of comparative example 2 and example 1, respectively, are as follows:
table 2 comparative data on seed germination rates in comparative example 2 and example 1
Figure SMS_2
As shown in Table 2, the Polygonatum cyrtonema seed can effectively promote seed germination by controlling the temperature at 15-20 ℃ in the germination stage.
Comparative example 3
This comparative example provides a facility seedling method of Polygonatum cyrtonema Fabricius seedlings, which is different from example 1 in that:
in S6 and S7, KH is not sprayed on the leaf surface independently in nutrient management 2 PO 4 Supplementing nutrients.
Other steps were consistent with example 1.
The comparison data of tuber weights at the stage of the one-leaf stage and the stage of the strong seedling stage of Polygonatum cyrtonema are as follows, using the seedling methods of comparative example 3 and example 1, respectively:
TABLE 3 comparison data of tuber weight at the stage of the one-leaf stage and the stage of the strong seedling stage in comparative example 3 and example 1
Figure SMS_3
As can be seen from Table 3, KH was sprayed on the leaf surfaces 2 PO 4 The foliar fertilizer can effectively improve the tuber weight in the first leaf stage and the seedling strengthening stage.
Comparative example 4
This comparative example provides a facility seedling method of Polygonatum cyrtonema Fabricius seedlings, which is different from example 1 in that:
the seedling substrate is prepared by mixing fungus residues, peat and perlite according to a ratio of 2:2:1, and other steps are consistent with those of the embodiment 1.
Comparative example 5
This comparative example provides a facility seedling method of Polygonatum cyrtonema Fabricius seedlings, which is different from example 1 in that:
the seedling substrate is prepared by mixing fungus residues, peat and perlite according to a ratio of 3:1:1, and other steps are consistent with those of the embodiment 1.
Comparative example 6
This comparative example provides a facility seedling method of Polygonatum cyrtonema Fabricius seedlings, which is different from example 1 in that:
the seedling substrate is prepared by mixing the residues, peat and perlite according to a ratio of 2:2:1, and other steps are consistent with those of the example 1.
Comparative example 7
This comparative example provides a facility seedling method of Polygonatum cyrtonema Fabricius seedlings, which is different from example 1 in that:
the seedling substrate is prepared by mixing the residues, peat and perlite according to a ratio of 3:1:1, and other steps are consistent with those of the embodiment 1.
Comparative example 8
This comparative example provides a facility seedling method of Polygonatum cyrtonema Fabricius seedlings, which is different from example 1 in that:
the seedling substrate is peat and perlite which are mixed according to a ratio of 3:1, and other steps are consistent with those of the embodiment 1.
The seedling methods of example 1 and comparative examples 4-8 were used, respectively, and the comparative data for the growth period of Polygonatum cyrtonema was as follows:
TABLE 4 comparative data during the growth of Polygonatum cyrtonema seedlings in example 1, comparative examples 4-8
Figure SMS_4
As can be seen from Table 4, the seedling stage adopts the seedling substrate of the invention to promote the growth of Polygonatum cyrtonema tuber, and provide sufficient nutrients for the growth of Polygonatum cyrtonema seedling.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention.

Claims (5)

1. The facility seedling raising method for polygonatum cyrtonema seedlings is characterized by comprising the following steps of:
s1, seed collection and cleaning: after the color of the polygonatum cyrtonema fruits is changed from green to black, collecting and filling the polygonatum cyrtonema fruits into a breathable woven bag; when the fruits are softened, the fruits are kneaded and rinsed to remove pulp and peel, and clean seeds are obtained;
s2, first disinfection of seeds: soaking the seeds treated in the step S1 in a disinfectant I for 1-2 hours, and draining excessive water;
s3, soaking seeds: soaking the seeds treated in the step S2 in clear water for 2-3 hours every day at 15-25 ℃, draining excessive water, and continuously operating for 10-20 days to ensure that the seeds are saturated with water until 3-6% of the seeds germinate;
s4, secondary disinfection of seeds: coating the seeds treated in the step S3 with a sterilizing seed coating agent for later use; the bactericidal seed coating agent is 62.5g/L of fine fenpropargyl or 4.8% of phenylate fludioxonil or 11% of fine fenpropargyl fludioxonil, the 62.5g/L of fine fenpropargyl fludioxonil and seeds are subjected to seed dressing according to the ratio of 1:250-333, the 4.8% of phenylate fludioxonil and the seeds are subjected to seed dressing according to the ratio of 1:350-500, and the 11% of fine fenpropargyl fludioxonil and the seeds are subjected to seed dressing according to the ratio of 200-400 ml/100 kg of seeds, wherein the percentage by mass is;
s5, managing germination period of the seeds in the first year: sowing the seeds treated in the step S4 in a foam box paved with 3-5CM storage matrixes, covering 1-2CM storage matrixes, covering the foam box, controlling the ambient temperature to 15-20 ℃ in the middle 3 months of the first year, keeping the matrix moisture to be 60-70% so as to promote the germination of primary roots and stems, managing under natural temperature conditions after the formation of the primary roots and stems, keeping the humidity unchanged, spraying trichoderma harzianum and potassium fulvate every 1 month after sowing, and promoting the growth of root systems and the expansion of the primary roots and stems;
s6, seedling management in the first leaf stage of the second year: after the primary rhizome enters the dormancy stage, disinfecting for standby, and then transferring to a plug tray I fully paved with seedling substrateOr in a flat plate, covering 1-2CM seedling substrate on the surface, placing the plug I or the flat plate in a greenhouse, keeping the humidity of the substrate at 40-70%, performing nutrient management and pest management, and controlling the shading degree at 65-75%, wherein the plug I is 200 holes; the nutrient management is that trichoderma harzianum and potassium fulvate are sprayed once every 20-30 days before emergence of seedlings for 60-70 days, root growth is promoted, and KH is sprayed on leaf surfaces every 15-20 days 2 PO 4 Supplementing nutrients once, and taking out seedlings for 70 days until the seedlings fall, and flushing and applying N: P: K balanced water-soluble fertilizer once every 20 days; the pest and disease control is that the emergence rate reaches 50%, carbendazim and mancozeb are alternately applied every 15 days, and the occurrence of diseases is prevented; when the foliar diseases occur, amikacin or procymidone or penthiopyrad or picoxystrobin is used for treatment; when the rhizome disease occurs, hymexazol, thifluzamide or fluxapyroxad is used for treatment; when insect damage occurs less in the greenhouse, the insect damage can be prevented and controlled in a physical mode;
s7, strong seedling management in the third year: sterilizing rhizoma Polygonati in one leaf stage after dormancy stage, transferring into tray II or flat plate full of seedling substrate, slightly pressing, covering 1-2cm seedling substrate, placing tray II or flat plate in greenhouse, maintaining moisture content of substrate at 40-70%, performing nutrient management and pest and disease control, and controlling shading degree at 45-55%，The plug II is 50 holes;
the nutrient management is that trichoderma harzianum and potassium fulvate are sprayed once every 20 days when the temperature of the greenhouse is above 15 ℃ to promote root growth for 2-3 times in total; spraying KH on the leaf surface 30 days before emergence 2 PO 4 Supplementing nutrients, and spraying once every 15 days; the seedling emergence lasts for 30 to 90 days, and the high-nitrogen type macroelement water-soluble fertilizer is applied once every 20 days, so that the growth of the leaves is promoted; the seedling emergence is carried out for 90 days to the seedling pouring, and the high-potassium type macroelement water-soluble fertilizer is applied once every 20 days, so that the rhizome expansion is promoted;
the pest and disease control is that the emergence rate reaches 50 percent, and carbendazim and mancozeb are alternately applied every 15 days to prevent diseases; when the foliar diseases occur, amikacin or procymidone or penthiopyrad or picoxystrobin is used for treatment; when the rhizome disease occurs, hymexazol, thifluzamide or fluxapyroxad is used for treatment; the pest in the greenhouse can be prevented and controlled in a physical mode when the pest is less.
2. The facility seedling raising method of polygonatum cyrtonema seedlings according to claim 1, wherein the disinfectant I in the step S2 is a carbendazim solution with the concentration of 0.2-0.3%.
3. The facility seedling raising method of Polygonatum cyrtonema Fabricius seedlings according to claim 1, wherein the storage substrate in the step S5 is peat and perlite which are mixed uniformly according to the proportion of 2-2.5:1, and soil disinfectant is used for disinfection for standby.
4. The facility seedling raising method of polygonatum cyrtonema seedlings according to claim 1, wherein the step S5 is completed indoors or in a greenhouse.
5. The facility seedling raising method of Polygonatum cyrtonema Fabricius seedlings according to claim 1, wherein the seedling raising substrate S6 is peat, coconut coir and perlite which are uniformly mixed according to the ratio of 3-4:2:1, and soil disinfectant is used for disinfection for standby.
CN202111414072.7A 2021-11-25 2021-11-25 Facility seedling raising method for polygonatum cyrtonema seedling Active CN114128578B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111414072.7A CN114128578B (en) 2021-11-25 2021-11-25 Facility seedling raising method for polygonatum cyrtonema seedling

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111414072.7A CN114128578B (en) 2021-11-25 2021-11-25 Facility seedling raising method for polygonatum cyrtonema seedling

Publications (2)

Publication Number Publication Date
CN114128578A CN114128578A (en) 2022-03-04
CN114128578B true CN114128578B (en) 2023-06-16

Family

ID=80391835

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111414072.7A Active CN114128578B (en) 2021-11-25 2021-11-25 Facility seedling raising method for polygonatum cyrtonema seedling

Country Status (1)

Country Link
CN (1) CN114128578B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115191305B (en) * 2022-08-02 2023-09-15 江西农业大学 Method for breaking dormancy rapid leaf growth of rhizoma polygonati seeds

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105248140A (en) * 2015-11-23 2016-01-20 陕西师范大学 Seedling culturing method for polygonatum cyrtonema seeds
CN110537468A (en) * 2019-09-25 2019-12-06 贵州百灵企业集团制药股份有限公司 simple polygonatum cyrtonema seed seedling culture method
CN111316786A (en) * 2020-04-26 2020-06-23 浙江省林业科学研究院 Polygonatum cyrtonema seed germination accelerating and seedling cultivation method
CN111557219A (en) * 2020-06-10 2020-08-21 湖南省农业环境生态研究所 Polygonatum cyrtonema seedling growing method
CN111937703B (en) * 2020-08-24 2022-08-26 三明市农业科学研究院 Method for breeding high-quality polygonatum cyrtonema seedlings

Also Published As

Publication number Publication date
CN114128578A (en) 2022-03-04

Similar Documents

Publication Publication Date Title
CN103918426B (en) A kind of high yield of loofah implantation methods
CN106376343A (en) Efficient ecological anniversary planting method of middle-season rice ratooning rice and astragalus smicus
CN105309166A (en) Towel gourd planting method
CN110574665B (en) Outdoor transplanting method for sealwort tissue culture seedlings
CN109997644B (en) One-year four-generation breeding technology of northern spring soybeans in northeast alpine regions
CN101627729B (en) High-quality corm of colorful calla and method for cultivating same
CN110178690B (en) Application of carbon nano sol in strawberry cultivation, strawberry seedling cultivation medium, matrix and strawberry seedling cultivation method
CN114128578B (en) Facility seedling raising method for polygonatum cyrtonema seedling
CN108934679B (en) Root-cutting seedling raising and seedling tube raising method for aralia elata seem
CN109220642A (en) A kind of paddy rice planting method
CN105993629A (en) Grafting method for increasing eggplant yield
CN110036851B (en) Pinellia ternata water-drought segmented seed breeding method
CN106900205A (en) A kind of ilex pubescens nursery propagation method
CN107409896B (en) Sowing and seedling raising method for golden panda trees
CN105612854A (en) Green planting method for cucumbers in solar greenhouse
CN111602486A (en) Seedling transplanting method for canna edulis ker seedling seeds
CN109315423A (en) A kind of HERBA DENDROBII seed germinant and preparation method thereof
CN110915577B (en) Angelica keiskei seedling transplanting method
CN100499997C (en) Ainsliaea fragrans champ breeding method
CN113875527A (en) Planting method for reducing occurrence of diseases of preserved szechuan pickle
CN112021089A (en) Seedling growing method of caulis Spatholobi Yunnanensis
CN111418431A (en) Simple method for breeding hispid fig seeds
CN104920083B (en) A kind of tea tree root reverse graft method
CN111434210A (en) Simple interplanting method for common head cabbages
CN110278790B (en) Rapid seedling method for sophora japonica

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant