CN111937703A - Method for breeding high-quality polygonatum cyrtonema seedlings - Google Patents
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Abstract
A method for breeding high-quality polygonatum cyrtonema seedlings mainly comprises the following steps: 1) preparing high-quality test-tube seedlings, 2) hardening the test-tube seedlings, 3) preparing before transplanting, 4) transplanting the test-tube seedlings, 5) managing water and fertilizer, 6) preventing and treating plant diseases and insect pests, and 7) shading management. The method for breeding the high-quality polygonatum cyrtonema seedlings is ideal in design, researches are carried out on aspects of high-quality test-tube seedling preparation, test-tube seedling hardening, preparation before transplanting, test-tube seedling transplanting, water and fertilizer management, pest control, shading management and the like, so that the method for breeding the high-quality polygonatum cyrtonema seedlings is provided, the survival rate and disease resistance of the test-tube seedlings are improved, labor force is saved, the seedling culture period is shortened, the quality of the polygonatum cyrtonema seedlings is improved, and the large-scale production of the high-quality polygonatum cyrtonem.
Description
Technical Field
The invention relates to a plant tissue culture and cultivation technology, in particular to a method for obtaining high-quality polygonatum cyrtonema seedlings by tissue culture and matched domestication cultivation technology.
Background
Polygonatum cyrtonema Hua (rhizoma Polygonati)Polygonatum cyrtonema The Huang of the Huang' an) is a medicinal plant of the polygonatum of the liliaceae, the rhizome of the medicinal plant is used as the traditional Chinese medicine polygonatum, the medicinal plant mainly contains sugar, steroid saponin, flavone, anthraquinone compounds, alkaloid, cardiac glycoside, lignan, vitamin, a plurality of amino acids and trace elements which are useful for human bodies and the like, has the effects of prolonging the service life, resisting aging, influencing the cardiovascular system, regulating the immunity, resisting inflammation and pathogenic microorganisms, resisting fatigue, improving the learning memory, inhibiting tumor cells and the like, and is safe, non-toxic and free of mutagenicity. With the gradual and deep research of people on pharmacology and pharmacotization of polygonatum, the polygonatum is more and more widely applied in the aspects of clinical application, health products, foods, cosmetics, ornamental value and the like.
Polygonatum cyrtonema has scattered cultivation in some areas in south China, and the Polygonatum cyrtonema is mainly propagated by asexual reproduction of roots and seeds, but large-area planting is limited because the propagation coefficient is low and the propagation period of the seeds is long and the speed is slow, so that a large number of seed sources cannot be provided. In addition, long-term asexual reproduction is easy to cause germplasm character degradation, and genetic stability of the variety is influenced. In recent years, with the increasing demand of polygonatum, wild resources are far from meeting the requirements, artificial cultivation is a main way for solving the problems, and high-quality seedlings become the bottleneck of artificial cultivation. The prior reports about polygonatum cyrtonema test-tube plantlet propagation technology can realize the propagation of polygonatum cyrtonema test-tube plantlet, but the overall propagation method is not ideal enough. Compared with the seed propagation or the conventional rhizome asexual propagation method, the test-tube plantlet rapid propagation method for the high-quality polygonatum cyrtonema is not restricted by natural weather conditions, improves the quality, increases the propagation coefficient, shortens the cultivation period and produces the high-quality test-tube plantlet in a large scale. The seedling of the seed propagation needs to be cultivated for at least 3 years to reach the standard of the seedling for production, the high-quality test-tube seedling needs to be cultivated for only 2 years to reach the standard of the seedling for production, and the cultivation period of one year can be shortened by using the high-quality test-tube seedling. Therefore, establishing and perfecting a high-quality polygonatum cyrtonema seedling breeding system becomes a problem which needs to be solved urgently in the current production, and is also one of the main measures for protecting and continuously utilizing wild germplasm resources.
Disclosure of Invention
The invention provides a method for breeding high-quality polygonatum cyrtonema seedlings, and aims to overcome the defects that the existing polygonatum cyrtonema cultivation method is not ideal enough, and the large-scale production requirement of polygonatum cyrtonema test-tube seedlings is difficult to meet.
A method for breeding high-quality polygonatum cyrtonema seedlings comprises the following steps:
a) preparing high-quality test-tube plantlets: selecting strong underground rhizomes of polygonatum cyrtonema as explants, and performing pretreatment and culture for multiple times to obtain high-quality test-tube plantlets for later use.
b) Hardening the test-tube seedlings: and (3) moving the test tube bottle containing the test tube plantlets to a greenhouse at normal temperature for hardening the plantlets for 60-90 days, then opening the bottle caps of the test tube bottles, hardening the plantlets for 2-4 days, and taking out the test tube plantlets.
c) Test-tube seedling rejuvenation: taking out the test-tube plantlet, cleaning agar on the surface of the test-tube plantlet, transplanting the test-tube plantlet into a seedling-recovering culture medium, recovering the seedling for 8-12 days under the condition that the temperature is controlled to be 12-22 ℃ and the temperature is kept under shade; the medium for seedling revival culture consists of coarse sand, perlite and plant ash.
d) Preparation before transplanting: the method comprises the steps of processing the test-tube plantlets, determining the transplanting time and screening the substrate proportion, wherein the test-tube plantlet processing mode is as follows: soaking the test-tube plantlet with mancozeb for 18-22 minutes for planting; determination of transplanting time: selecting to seed from 11 months later in the current year to 1 month earlier in the next year; screening the mixture ratio of the matrix: the matrix is prepared by mixing peat soil and organic fertilizer, and the matrix is prepared by mixing peat soil: preparing an organic fertilizer =5: 1; the base is finely leveled by using a rake, a buckle plate is used as an edge, a timber pile is fixed, a seedling raising bed is made, and the leveling is carried out after a prepared substrate is filled.
e) Transplanting test-tube seedlings: uniformly spreading the test tube seedlings in the step c) on the ridge with the row spacing of 1.5-2.0cm x 1.5-2.0cm as far as possible, uniformly spreading matrix soil with the thickness of 1.5-2cm above the test tube seedlings, and finally covering the test tube seedlings with pine needles with the thickness of about 0.8-1 cm.
f) And (3) water and fertilizer management: after transplanting, watering the root fixing water for one time until the root fixing water is immersed in the soil for about 10 centimeters, and then watering the root fixing water for one time in a mode of installing sprinkling irrigation for 1 week. After the test-tube plantlet emerged, the test-tube plantlet was cultured in 1000 times of solution N: p: the compound fertilizer with K =15:15:15 is generally watered with liquid fertilizer once a week in spring without watering. Water and fertilizer are poured once a week in summer and autumn until the seedlings are poured.
g) And (3) pest control: after the first seedling emergence, spraying the Gendazole once a day for 3 times continuously to enhance the photosynthesis and disease resistance of leaf surfaces; the pest is mainly aphids, and imidacloprid is beaten twice in the seedling emergence stage and the seedling stage of the test-tube seedlings to prevent and control the aphids; the disease control adopts the principle of fungus control, trichoderma and bacillus are prepared into a mixed biocontrol strain with the ratio of 1:1, the mixed biocontrol strain is sprayed once every 30d-45d, and the application concentration is controlled between 5 multiplied by 10^6cfu/mL to 8 multiplied by 10^6 cfu/mL.
h) Shading management: before and after seedling emergence of the test-tube seedlings, in spring and in cloudy days, shading is not needed, and a layer of shading net with the concentration of 50% is needed to cover from summer to before seedling falling.
Further, the culture medium prepared from the high-quality test-tube plantlet in the step a) comprises a proliferation and strong seedling culture medium, a differentiation culture medium and a rooting culture medium which are used for sequentially culturing explants.
Further, the test-tube plantlet transplanting condition in the step e) is selected in a greenhouse with an intelligent water curtain fan, and the temperature is controlled to be 25 +/-2 ℃.
From the above description of the present invention, it can be seen that the advantages of the present invention over the prior art are: the method for breeding the high-quality polygonatum cyrtonema seedlings is ideal in design, researches are carried out on aspects of high-quality test-tube seedling preparation, test-tube seedling hardening, preparation before transplanting, test-tube seedling transplanting, water and fertilizer management, pest control, shading management and the like, so that the method for breeding the high-quality polygonatum cyrtonema seedlings is provided, the survival rate and disease resistance of the test-tube seedlings are improved, labor force is saved, the seedling culture period is shortened, the quality of the polygonatum cyrtonema seedlings is improved, and the large-scale production of the high-quality polygonatum cyrtonem. Compared with seed seedling and wild seed stem, the tissue fast propagation process can produce test tube seedling in large scale without influence of season and weather. The water and fertilizer management mode of the high-quality polygonatum cyrtonema seedling breeding method has the advantages that moisture and fertilizer are preserved, the survival rate of test-tube seedlings can reach more than 90%, after medicine soaking, the disease resistance of the test-tube seedlings is improved, and the disease resistance of the test-tube seedlings is stronger. The high-quality polygonatum cyrtonema seedling breeding method adopts a broadcast sowing mode, greatly reduces the planting time, saves the labor force and improves the labor efficiency. The seedling breeding method of the high-quality polygonatum cyrtonema shortens the seedling breeding period, the seedling and seeds need to be planted for 3 years to reach the seed stem standard (the diameter of a tuber is more than 2 cm), the test-tube seedling only needs 1.5 to 2 years, and the seedling breeding period is shortened by at least 1 year. As shown in FIG. 1, the tubers of the high quality tissue-cultured rooted seedlings were stronger than the annual seed seedlings. As shown in FIG. 2, the biennial high quality seedlings have reached the seed stem standard. The quality Polygonatum cyrtonema seedling breeding method can improve the quality of Polygonatum cyrtonema seedlings, and compared with seedling and wild seed stems, the test-tube seedlings have the advantages of consistent quality, neat seedlings, stable quality and the like.
Drawings
FIG. 1 is a diagram showing the effect of the test-tube plantlet of the present invention.
FIG. 2 is a diagram showing the effect of comparison between the high-quality test-tube plantlet before hardening off and the high-quality test-tube plantlet after hardening off, wherein the test-tube plantlet on the left side is the high-quality test-tube plantlet after hardening off, and the test-tube plantlet on the right side is the high-quality test-tube plantlet before hardening off.
FIG. 3 is a graph showing the effect of comparison between the 2-year-old tissue culture seedlings of the present invention and the conventional 2-year-old seed seedlings.
Detailed Description
A method for breeding high-quality polygonatum cyrtonema seedlings comprises the following steps:
a) preparing high-quality test-tube plantlets: selecting strong underground rhizomes of polygonatum cyrtonema as explants, and performing pretreatment and culture-based multiple culture to obtain high-quality test-tube plantlets for standby, wherein reference is made to the attached figure 1 in the specification; the culture medium comprises a proliferation and seedling strengthening culture medium, a differentiation culture medium and a rooting culture medium which are used for sequentially culturing explants.
b) Hardening the test-tube seedlings: and (3) moving the test tube bottle containing the test tube seedling to a normal-temperature greenhouse to harden the seedling for 60-90 days (the optimal day is 85 days), then opening the bottle cap of the test tube bottle, hardening the seedling for 2-4 days (the optimal day is 3 days), taking out the test tube seedling, gently taking out the bud body by using tweezers when the seedling is taken, cleaning the culture medium, draining water for later use, and referring to the attached figure 2 of the specification.
c) Test-tube seedling rejuvenation: taking out the test-tube plantlet, cleaning agar on the surface of the test-tube plantlet, transplanting the test-tube plantlet into a seedling-recovering culture medium, controlling the temperature to be 12-22 ℃ under a shady condition, recovering the seedling for 8-12 days for later use; the medium for seedling revival culture consists of coarse sand, perlite and plant ash.
d) Preparation before transplanting: the method comprises the steps of processing the test-tube plantlets, determining the transplanting time and screening the substrate proportion, wherein the test-tube plantlet processing mode is as follows: soaking the test-tube plantlet with mancozeb for 18-22 minutes for planting; determination of transplanting time: seeds are planted from 11 months of the current year to 1 month of the next year, the weather is cool in this period, moisture is easy to keep, the survival rate of test-tube seedlings can reach more than 90%, and the seedlings grow well; screening the mixture ratio of the matrix: the matrix is prepared by mixing peat soil and organic fertilizer, and the matrix is prepared by mixing peat soil: preparing an organic fertilizer =5: 1; finely leveling the base by using a rake, taking a buckle plate (with the height of 30 cm and the length of 3 m) as a side, fixing a wood pile, making a seedling raising bed (with the width of 60 cm), filling a prepared substrate (with the height of 8-10 cm), and leveling.
e) Transplanting test-tube seedlings: and c), uniformly broadcasting the test tube seedlings in the step c) on the furrows as much as possible, wherein the row spacing is 1.5-2.0cm x 1.5-2.0cm (such as 2.0cm x 2.0 cm), uniformly scattering matrix soil above the test tube seedlings, the thickness of the matrix soil is about 1.5-2cm, finally covering the test tube seedlings with pine needles, the thickness of the pine needles is about 0.8-1 cm, in cold seasons in winter, covering the furrows with a layer of straws for heat preservation, and lifting off the test tube seedlings before seedling emergence in the next year. The test-tube seedling transplanting condition is selected from a greenhouse with an intelligent water curtain fan, the temperature is controlled to be 25 +/-2 ℃, the cultivation is performed in a light scattering mode, and the illumination intensity is 4000 lx.
f) And (3) water and fertilizer management: after transplanting, watering the root fixing water for one time until the root fixing water is immersed in the soil for about 10 centimeters, and then watering the root fixing water for one time in a mode of installing sprinkling irrigation for 1 week. After the test-tube plantlet emerged, the test-tube plantlet was cultured in 1000 times of solution N: p: the compound fertilizer with K =15:15:15 is generally watered with liquid fertilizer once a week in spring without watering. Water and fertilizer are poured once a week in summer and autumn until the seedlings are poured.
g) And (3) pest control: spraying 1000 times of radix Dahliang (which can make main root of medicinal material stronger, fibrous root vigorous, high yield and good quality) once a day after primary seedling emergence for 3 times continuously to enhance photosynthesis and disease resistance of leaf surface; the pest is mainly aphids, and imidacloprid is beaten twice in the seedling emergence stage and the seedling stage of the test-tube seedlings to prevent and control the aphids; the disease control adopts the principle of fungus control, trichoderma and bacillus are prepared into a mixed biocontrol strain with the ratio of 1:1, the mixed biocontrol strain is sprayed once every 30d-45d, and the application concentration is controlled between 5 multiplied by 10^6cfu/mL to 8 multiplied by 10^6 cfu/mL.
h) Shading management: before and after seedling emergence of the test-tube seedlings, in spring and in cloudy days, shading is not needed, and a layer of shading net with the concentration of 50% is needed to cover from summer to before seedling falling.
i) And (4) weeding management: the grass is pulled out periodically, the grass is cut to grow up and then pulled out, the root system of the test-tube plantlet is easy to be hurt when the grass is pulled out, and the dead leaves are cleaned in time after the test-tube plantlet is poured out. The effect chart of the comparison between the 2-year tissue culture seedling prepared by the method and the conventional 2-year seed seedling can refer to the attached figure 3 in the specification.
The preparation of the high-quality test-tube plantlet in the step a) specifically comprises the following steps: A) selecting and pretreating explants: selecting strong underground rhizomes of polygonatum cyrtonema planted for 2-3 years as explants in 9-10 months of the current year; the explant pretreatment comprises the steps of sequentially cleaning, cutting excess materials and disinfecting the explant, and specifically comprises the following steps: cleaning underground rhizomes (namely explants) of polygonatum cyrtonema with water, removing leaves and roots, cutting off redundant rhizomes around, removing bracts which can be stripped from buds, and taking middle rootstock blocks with buds, wherein the volume of the rootstock blocks with buds is about 1.0cm multiplied by 0.5 cm; then washing the rhizomes with buds with flowing tap water for 1-2 hours, and airing the rhizomes; soaking in 75% ethanol for 15s on a clean bench, washing with sterile water for 2-4 times, adding into 0.1% mercuric chloride, shaking for 8-10min, taking out, washing with sterile water for 5-8 times, and drying with sterile filter paper.
B) And (3) proliferation and strong seedling culture: cutting off bud tops of the disinfected rootstock blocks with buds by using a scalpel, inoculating the rootstock blocks with buds to a proliferation and strong seedling culture medium for culture, expanding the periphery of rootstocks after 10 days, growing callus, and then successively emitting bud points until tubers expand, the buds are thick and the color of the tubers is deepened to obtain a first rootstock block; culturing on the proliferation strong seedling culture medium for 42-47 days (the optimal days are 45 days); the culture medium for proliferating strong seedlings comprises: MS + 1.0 mg/L6-BA + 0.5mg/L NAA + sugar 90g/L, the pH value of said culture medium is controlled at 5.5-5.7 (optimum pH value is 5.6); the culture medium for proliferating strong seedlings has the characteristics that: can effectively inhibit the leaf extraction of the rootstock blocks with buds, the dormancy of buds, no leaf extraction, the top advantages are removed, and the nutrition is supplied to the rootstock in a centralized way, so the rootstock expansion speed is high.
C) Differentiation culture: cutting the first rootstock block into a first unit rootstock block with uniform volume (the volume is about 1.0cm multiplied by 0.5 cm), inoculating the first unit rootstock block into a differentiation culture medium for culture, expanding the periphery of the rootstock block after 7 days, and emitting a plurality of buds on the periphery until the buds extend and leaves are extracted to obtain a second rootstock block; the differentiation medium is as follows: MS + 1.0 mg/L6-BA + 0.5mg/L NAA + 0.2mg/L IBA + sugar 30g/L, the pH value of the differentiation culture medium is controlled at 5.5-5.7 (optimum pH value is 5.6); and cutting bud points of the second rhizome block by using a scalpel, cutting the second rhizome block into second unit rhizome blocks of 0.5cm multiplied by 0.5cm, inoculating the second unit rhizome blocks into the proliferation strong seedling culture medium, and performing circulating culture for several generations, so that a large number of second rhizome blocks (namely high-quality bottle seedlings) can be obtained in a short time. The differentiation culture medium has the characteristics that: the growth speed of the rootstocks is high, and the buds can be stretched and pulled out of the leaves.
D) Rooting culture: and cutting the second rhizome block into third rhizome blocks with uniform volume (about 1.5cm multiplied by 1.5 cm), ensuring that each third rhizome block has at least a root stem above 1 healthy bud, and transferring the third rhizome blocks to a rooting culture medium for culture to obtain the high-quality test-tube plantlet. The rooting culture medium comprises: MS + 1.0 mg/L6-BA + 0.5mg/L NAA + 0.2mg/L IBA + activated carbon 0.5g/L sugar 30g/L, the pH value of the rooting medium is controlled between 5.5 and 5.7 (optimum pH value is 5.6). The rooting culture medium has the characteristics that: can obtain strong polygonatum cyrtonema high-quality test-tube plantlet, and refer to the attached figure 1 of the specification specifically.
Example two
The present embodiment is substantially the same as the first embodiment, except that: preparing before transplanting in the step d): screening the mixture ratio of the matrix: the matrix is prepared by mixing peat, sand and loess (the peat, sand and loess are mixed according to the proportion of 1:1: 1). Finely leveling the base by using a rake, taking a buckle plate (with the height of 30 cm and the length of 3 m) as a side, fixing a wood pile, making a seedling raising bed (with the width of 60 cm), filling the prepared matrix (with the height of 8-10 cm), and leveling. The method comprises the steps of carrying out a proportioning test by using peat soil, loess and sand which are prepared according to a ratio of 1:1, garden soil, peat and sand which are prepared according to a ratio of 1:1:1 and loess and peat and sand which are prepared according to a ratio of 3:1 as matrixes (transplanting No. 3/month No. 13-10/month No. 13 in 2019, repeating for 3 times, and counting No. 4/month No. 25 in 2020). The survival rate of the peat soil is 76.7 percent, 56.7 percent and 66.7 percent, and the average survival rate is 66.7 percent; the survival rate of loess and sand is =1:1, the survival rate is 76.7%, 60.0% and 73.3%, and the average survival rate is 70.0%; the survival rate of the garden soil is 20.0 percent, 16.7 percent and 20.0 percent, and the average survival rate is 18.9 percent; the survival rate of peat, sand and loess is 1:1:1, the survival rate is 90.0 percent, 83.3 percent and 86.7 percent, and the average survival rate is 86.7 percent; the survival rate of peat and sand =3:1 is 73.3%, 66.7% and 70.0%, and the average survival rate is 70%. In conclusion, the survival rate of peat, sand and loess is as high as 1:1:1, and the average survival rate is 86.7%.
The above description is only an embodiment of the present invention, but the design concept of the present invention is not limited thereto, and any insubstantial modifications made by using the design concept should fall within the scope of infringing the present invention.
Claims (4)
1. A method for breeding high-quality polygonatum cyrtonema seedlings is characterized by comprising the following steps:
a) preparing high-quality test-tube plantlets: selecting strong underground rhizomes of polygonatum cyrtonema as explants, and performing pretreatment and culture-based multiple culture to prepare high-quality test-tube plantlets for later use;
b) hardening the test-tube seedlings: transferring the test tube bottle containing the test tube plantlet to a greenhouse at normal temperature, hardening the plantlet for 60-90 days, opening the bottle cap of the test tube bottle, hardening the plantlet for 2-4 days, and taking out the test tube plantlet;
c) preparation before transplanting: the method comprises the steps of processing the test-tube plantlets, determining the transplanting time and screening the substrate proportion, wherein the test-tube plantlet processing mode is as follows: soaking the test-tube plantlet with mancozeb for 18-22 minutes for planting; determination of transplanting time: selecting to seed from 11 months later in the current year to 1 month earlier in the next year; screening the mixture ratio of the matrix: the matrix is prepared by mixing peat soil and organic fertilizer, and the matrix is prepared by mixing peat soil: preparing an organic fertilizer =5: 1; finely leveling the base by using a rake, using a buckle plate as an edge, fixing a timber pile, making seedling beds, filling a prepared matrix and leveling;
d) transplanting test-tube seedlings: uniformly spreading the test-tube plantlets on the ridges as much as possible, wherein the row spacing is 1.5-2.0cm x 1.5-2.0cm, uniformly spreading matrix soil above the test-tube plantlets, the thickness is 1.5-2cm, and finally covering with pine needles, wherein the thickness is about 0.8-1 cm;
e) and (3) water and fertilizer management: after transplanting, watering the root fixing water once, and watering until the root fixing water is immersed in the soil for about 10 centimeters, and then watering once in 1 week by adopting a spray irrigation mounting mode, and after the test-tube seedlings grow seedlings, using 1000 times of liquid N: p: the compound fertilizer with K =15:15:15 is prepared by pouring liquid fertilizer once a week in spring without watering, and pouring water and fertilizer once a week in summer and autumn until seedlings are poured;
f) and (3) pest control: after the first seedling emergence, spraying the Gendazole once a day for 3 times continuously to enhance the photosynthesis and disease resistance of leaf surfaces; the pest is mainly aphids, and imidacloprid is beaten twice in the seedling emergence stage and the seedling stage of the test-tube seedlings to prevent and control the aphids; the disease control adopts the principle of fungus control, trichoderma and bacillus are prepared into a mixed biocontrol strain with the ratio of 1:1, the mixed biocontrol strain is sprayed once every 30d-45d, and the application concentration is controlled between 5 multiplied by 10^6cfu/mL to 8 multiplied by 10^6 cfu/mL;
g) shading management: before and after seedling emergence of the test-tube seedlings, in spring and in cloudy days, shading is not needed, and a layer of shading net with the concentration of 50% is needed to cover from summer to before seedling falling.
2. The method for breeding high-quality polygonatum cyrtonema seedlings according to claim 1, wherein the method comprises the following steps: after the step b), test-tube plantlet rejuvenation is carried out: taking out the test-tube plantlet, cleaning agar on the surface of the test-tube plantlet, transplanting the test-tube plantlet into a seedling-recovering culture medium, recovering the seedling for 8-12 days under the condition that the temperature is controlled to be 12-22 ℃ and the temperature is kept under shade; the medium for seedling revival culture consists of coarse sand, perlite and plant ash.
3. The method for breeding high-quality polygonatum cyrtonema seedlings according to claim 1, wherein the method comprises the following steps: and d) selecting the conditions for transplanting the test tube seedlings in the step d) in a greenhouse with an intelligent water curtain fan, and controlling the temperature to be 25 +/-2 ℃.
4. The method for breeding high-quality polygonatum cyrtonema seedlings according to claim 1, wherein the method comprises the following steps: the culture medium prepared from the high-quality test-tube plantlet in the step a) comprises a proliferation and strong-seedling culture medium, a differentiation culture medium and a rooting culture medium which are used for sequentially culturing explants.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112703871A (en) * | 2020-12-29 | 2021-04-27 | 镇江常青农林科技有限公司 | Fertilizing method in growth process of polygonatum sibiricum |
| CN114128578A (en) * | 2021-11-25 | 2022-03-04 | 重庆市药物种植研究所 | Facility seedling raising method for polygonatum cyrtonema seedlings |
| CN115005038A (en) * | 2022-07-16 | 2022-09-06 | 江西农业大学 | Method for rapidly inducing multiple buds of rhizoma polygonati |
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