CN102487698A - Method for promoting chrysanthemum to bloom in advance by using 5-azaC - Google Patents
Method for promoting chrysanthemum to bloom in advance by using 5-azaC Download PDFInfo
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- CN102487698A CN102487698A CN2011104139630A CN201110413963A CN102487698A CN 102487698 A CN102487698 A CN 102487698A CN 2011104139630 A CN2011104139630 A CN 2011104139630A CN 201110413963 A CN201110413963 A CN 201110413963A CN 102487698 A CN102487698 A CN 102487698A
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Abstract
The invention relates to a method for promoting chrysanthemum to bloom in advance by using 5-azaC. The method comprises the following steps of: cutting and raising a seedling; after rooting, planting in a cultivation pot, and adopting the conventional cultivation management method to ensure water and fertilizer; and when a chrysanthemum plant has the height of 20-40cm, treating the apical meristem of the chrysanthemum by using the 5-azaC at the concentration of 200-500mu mol/l for 10 to 15 days, and restoring normal management. The method is easy to implement, has an obvious effect (the chrysanthemum can bloom 10 to15 days earlier), cannot generate any adverse effect on the enjoyment effect of the chrysanthemum, and is suitable for scale production in field.
Description
Technical field
The present invention relates to the regulate and control method at a kind of plant florescence, be specifically related to the method that a kind of 5-azaC of utilization promotes that chrysanthemum is bloomed in advance.
Background technology
Chrysanthemum is divided into spring chrysanthemum (late April at florescence is to late May) according to the nature florescence; Xia Ju (late May at florescence is to August); Qiu Ju (late October at florescence is to late November); Winter chrysanthemum (February of early December at florescence to next year).Chrysanthemum is wide in variety because of it, the flower type is good, nice selection becomes cultivation kind the most widely.Chrysanthemum grow, germinate the differentiation and bloom, with environmental factors such as sunshine, temperature and cultivations extremely close relation is arranged.Chrysanthemum is carried out regulation and control such as artificial sunshine, temperature, chrysanthemum is done sth. in advance or the delay florescence according to people's wish.Florescence is called " forcing culture " ahead of time than the nature florescence, and the florescence then is called " suppressing cultivation " than nature flowering delay.
The complete life cycle of plant is divided into nourishes and grows and two stages of reproductive growth, wherein is called " becoming flower to change " by the conversion process of nourishing and growing to reproductive growth, and the key of this process is floral induction.The floral induction process of higher plant is by self genetic material and the decision of external environment factor two aspects.Chrysanthemum is the short-day flowers, under the long-day condition, accomplishes and nourishes and grows, and under the short-day condition, carries out reproductive growth.In order to satisfy people's demand to chrysanthemum when non-natural florescence; Can according to chrysanthemum the physiological property of short-day plant; The artificial chrysanthemum growing environment of transforming is perhaps transformed chrysanthemum hereditary information, impels chrysanthemum to accelerate to grow, and turns to reproductive growth from nourishing and growing; Cause flower bud differentiation, thereby reach the purpose of blooming in advance.
Light application time is an envirment factor important in the chrysanthemum growth and development process; Flower bud differentiation plays an important role to chrysanthemum; Tradition chrysanthemum florescence control technique is exactly to utilize the requirement of chrysanthemum flower bud differentiation to light application time and intensity, handles that through shading or light filling chrysanthemum was bloomed at the improper florescence.This technology is strict to illumination condition, must carry out continuously, interrupts or handle at interval all causing short-day to handle failure.In addition, this operating process is loaded down with trivial details, and the short-day processing time is long, and time-consuming, effort is not easy to the land for growing field crops large-scale production.Chinese patent CN200810049322 discloses a kind of method of regulating florescence of chrysanthemum, and this method is transplanted with the test-tube plantlet of 5-aza-cytidine (5-azaC) processing chrysanthemum tissue culture then, and chrysanthemum is bloomed in advance.This method is because of relating to tissue culture, and is the processing time long (needing 30 days), also more loaded down with trivial details.
Summary of the invention
The object of the invention is to provide a kind of 5-azaC of utilization to promote the method that chrysanthemum is bloomed in advance, and method is simple for this, under the prerequisite that does not influence the chrysanthemum appreciation effect, chrysanthemum bloomed in advance.
For realizing above-mentioned purpose, the present invention adopts following technical scheme:
A kind of 5-azaC of utilization promotes the method that chrysanthemum is bloomed in advance; Comprise cuttage and seedling culture; Be colonizated in after taking root in the cultivation alms bowl, adopt conventional cultivation management method to guarantee that liquid manure is sufficient, when the chrysanthemum plant height is 20～40cm; Use methylation inhibitor 5-aza-cytidine (5-azaC) to handle the apical meristem of chrysanthemum, handle the back and recover normal management.
Said apical meristem with methylation inhibitor 5-azaC processing chrysanthemum is specially: use cotton balls absorption 400～500 μ l concentration are that the 5-azaC (water is mixed with respective concentration) of 200～500 μ mol/l is placed on chrysanthemum plant terminal bud (also can adopt the mode that sprays to carry out medication); Once a day, handled 10-15 days.
Preferably, cultivation in the alms bowl used cultivation matrix can for: volume ratio is refuse soil, the peat composed of rotten mosses and the chicken manure of 3:1:1; Conventional cultivation management comprises timely excision side shoot, lateral bud, superfluous bud and smears flower bud.
Further, the said 5-azaC of utilization promotes the method that chrysanthemum is bloomed in advance, in the early June cuttage and seedling culture; Be colonizated in after taking root in the cultivation alms bowl; Adopt conventional cultivation management method to guarantee that liquid manure is sufficient, when the chrysanthemum plant height is 25～35cm (this moment is generally to the first tenday period of a month in August), using cotton balls to draw 500 μ l concentration is that the 5-azaC of 200～500 μ mol/l is placed on chrysanthemum plant terminal bud; Once a day, keep recovering normal management after 10 days.
The inventive method is to adopt medicine 5-azaC to handle the chrysanthemum apical meristem after a period of time; Through changing the chrysanthemum epigenetic information; Promote that chrysanthemum gets into reproductive growth in advance after completion is nourished and grown; Can remedy the defective of light, temperature control technology like this, regulating florescence of chrysanthemum is bloomed it in advance more easily.Adopt the inventive method can exempt the complicated work that the flower-grower carries out shading or light filling every day, and this method is simple and easy to operate in implementation process, does not need the professional and technical personnel to implement.Handle change chrysanthemum epigenetic information through 5-azaC and realize blooming in advance, treatment effect is more stable, has also reduced short-day and has handled the risk that causes, and is more suitable for the large tracts of land production application.
The inventive method solved the operating process of traditional chrysanthemum florescence control technique loaded down with trivial details, have a big risk, time-consuming, the effort problem; And realized the purpose (chrysanthemum can shift to an earlier date 10-15 days and bloom) that promotion is bloomed in advance equally, can be for giving security with flower during " 11 " National Day.This method is simple to operate, and effect is obvious, can not produce any negative effect to the chrysanthemum appreciation effect, is applicable to the land for growing field crops large-scale production.
Embodiment
Below in conjunction with embodiment technical scheme of the present invention is done to introduce in detail further, but protection scope of the present invention is not limited thereto.
Embodiment 1
Present embodiment is that " hydrangea " chrysanthemum kind is made an experiment, and concrete control method is: early June cuttage and seedling culture, (cultivation matrix in the cultivation alms bowl consists of V to be colonizated in the cultivation alms bowl after taking root
Refuse soil: V
The peat composed of rotten mosses: V
Chicken manure=3:1:1), cultivation alms bowl bore 19cm, high 15cm.Guarantee in vegetative period that liquid manure is sufficient, in time extract side shoot, lateral bud, superfluous bud, in time smear chrysanthemum of flower bud assurance and open a flower, be convenient to add up flowering time (be colonizated in the cultivation alms bowl from the chrysanthemum seedling and counted first day the same day).August 15 is when being 30cm when chrysanthemum seedling plant height; Experimental group is drawn the 5-azaC aqueous solution that 500 μ l concentration are respectively 200,250,300,400 and 500 μ mol/l with cotton balls and is placed on chrysanthemum plant terminal bud; Every day, processing once (was changed treatment fluid every afternoon 1 time); Recover normal management after continuous 10 days, (it is that standard is added up that flowering time is told look with bud to the flowering time in the record process of the test, and it is that bud has just shown colored color that so-called bud is told look; Down together).Control group is smeared distilled water (5-azaC concentration is counted 0 μ mol/l), and result of the test is seen table 1.
Embodiment 2
Present embodiment is that " the spring township is soaring " chrysanthemum kind is made an experiment, and concrete control method is: early June cuttage and seedling culture, (cultivation matrix in the cultivation alms bowl consists of V to be colonizated in the cultivation alms bowl after taking root
Refuse soil: V
The peat composed of rotten mosses: V
Chicken manure=3:1:1), cultivation alms bowl bore 19cm, high 15cm.Guarantee in vegetative period that liquid manure is sufficient, in time extract side shoot, lateral bud, superfluous bud, in time smear chrysanthemum of flower bud assurance and open a flower, be convenient to add up flowering time (be colonizated in the cultivation alms bowl from the chrysanthemum seedling and counted first day the same day).August 15 is when being 30cm when chrysanthemum seedling plant height; Draw the 5-azaC aqueous solution that 500 μ l concentration are respectively 200,250,300,400 and 500 μ mol/l with cotton balls and be placed on chrysanthemum plant terminal bud; Every day, processing once (was changed treatment fluid every afternoon 1 time), recovered normal management after continuous 10 days.Control group is smeared distilled water (5-azaC concentration is counted 0 μ mol/l), and result of the test is seen table 2.
Experimental result proves: smear after the top handles with the 5-azaC of concentration 200～500uM for different chrysanthemum kinds; All can make chrysanthemum bloom in advance (in advance 10～15 days) to a certain extent; And the method is time saving and energy saving, simple to operation, has remedied the deficiency of light, warm regulation florescence.
It should be noted last that: above embodiment is only in order to explanation; And unrestricted technical scheme of the present invention; Although the present invention is specified with reference to the foregoing description; Those of ordinary skill in the art is to be understood that: still can make amendment or be equal to replacement the present invention, and not break away from any modification or the local replacement of the spirit and scope of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (4)
1. one kind is utilized 5-azaC to promote the method that chrysanthemum is bloomed in advance; Comprise cuttage and seedling culture, be colonizated in after taking root in the cultivation alms bowl, adopt conventional cultivation management method to guarantee that liquid manure is sufficient; It is characterized in that; When the chrysanthemum plant height is 20～40cm, use methylation inhibitor 5-azaC to handle the apical meristem of chrysanthemum, handle the back and recover normal management.
2. utilize 5-azaC to promote the method that chrysanthemum is bloomed in advance according to claim 1; It is characterized in that: said apical meristem with methylation inhibitor 5-azaC processing chrysanthemum is specially: use cotton balls absorption 400～500 μ l concentration are that the 5-azaC of 200～500 μ mol/l is placed on chrysanthemum plant terminal bud; Once a day, handled 10-15 days.
3. utilize 5-azaC to promote the method that chrysanthemum is bloomed in advance according to claim 1, it is characterized in that: used cultivation matrix is in the cultivation alms bowl: volume ratio is refuse soil, the peat composed of rotten mosses and the chicken manure of 3:1:1; Conventional cultivation management comprises timely excision side shoot, lateral bud, superfluous bud and smears flower bud.
4. utilize 5-azaC to promote the method that chrysanthemum is bloomed in advance according to claim 1, it is characterized in that, in the early June cuttage and seedling culture; Be colonizated in after taking root in the cultivation alms bowl; Adopt conventional cultivation management method to guarantee that liquid manure is sufficient, when the chrysanthemum plant height was 25～35cm, using cotton balls to draw 500 μ l concentration was that the 5-azaC of 200～500 μ mol/l is placed on chrysanthemum plant terminal bud; Once a day, keep recovering normal management after 10 days.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104705083A (en) * | 2015-04-14 | 2015-06-17 | 河南大学 | Method for promoting early blossoming of chrysanthemum by utilizing curcumin |
| CN105580806A (en) * | 2016-02-15 | 2016-05-18 | 河南师范大学 | Chrysanthemum flowering phase composite regulation agent and method for regulating and controlling chrysanthemum flowering phase |
| CN107950540A (en) * | 2017-11-22 | 2018-04-24 | 青岛农业大学 | U-18496 is being prepared for preventing by the purposes in the fungicide of the microbial plant disease of pathogenic |
| CN108901580A (en) * | 2018-07-25 | 2018-11-30 | 河南科技大学 | A method of promoting eary maturity of grape |
| CN110042120A (en) * | 2019-05-07 | 2019-07-23 | 沈阳农业大学 | The method for improving soybean resistance inducing clumping bud efficiency using 5- aza-cytidine |
| CN111034482A (en) * | 2020-01-15 | 2020-04-21 | 河南大学 | Method for treating chrysanthemum cutting to promote chrysanthemum to bloom in advance |
| CN115735772A (en) * | 2022-12-01 | 2023-03-07 | 青岛农业大学 | Application of 5-AzAC in rooting culture of apple rootstock tissues or sugar-free rooting culture |
| CN117204220A (en) * | 2023-09-13 | 2023-12-12 | 扬州大学 | Treatment method for improving seed production rate of cucumber seed melon |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01235527A (en) * | 1988-03-14 | 1989-09-20 | Akita Pref Gov | Dwarfing of normal plant using 5-azacitidine |
| CN101347075A (en) * | 2008-03-07 | 2009-01-21 | 河南大学 | Method for regulating florescence of chrysanthemum |
| CN101755582A (en) * | 2009-09-29 | 2010-06-30 | 孔赟荣 | Chrysanthemum out-of-season blooming cultivation technique and method |
-
2011
- 2011-12-13 CN CN 201110413963 patent/CN102487698B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01235527A (en) * | 1988-03-14 | 1989-09-20 | Akita Pref Gov | Dwarfing of normal plant using 5-azacitidine |
| CN101347075A (en) * | 2008-03-07 | 2009-01-21 | 河南大学 | Method for regulating florescence of chrysanthemum |
| CN101755582A (en) * | 2009-09-29 | 2010-06-30 | 孔赟荣 | Chrysanthemum out-of-season blooming cultivation technique and method |
Non-Patent Citations (3)
| Title |
|---|
| 李新玲等: "植物DNA甲基化与表观遗传", 《中国农学通报》, no. 1, 31 January 2008 (2008-01-31), pages 123 - 126 * |
| 王子成等: "离体条件下5_氮杂胞嘧啶核苷对菊花DNA甲基化和表型性状的影响", 《园艺学报》, vol. 36, no. 12, 31 December 2009 (2009-12-31), pages 1783 - 1790 * |
| 聂丽娟等: "DNA甲基化抑制剂作用机理及其在植物发育生物学研究中的应用", 《核农学报》, no. 4, 31 August 2007 (2007-08-31), pages 362 - 365 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104705083A (en) * | 2015-04-14 | 2015-06-17 | 河南大学 | Method for promoting early blossoming of chrysanthemum by utilizing curcumin |
| CN105580806A (en) * | 2016-02-15 | 2016-05-18 | 河南师范大学 | Chrysanthemum flowering phase composite regulation agent and method for regulating and controlling chrysanthemum flowering phase |
| CN105580806B (en) * | 2016-02-15 | 2018-09-28 | 河南师范大学 | A kind of method of chrysanthemum florescence composite regulator and regulating florescence of chrysanthemum |
| CN107950540A (en) * | 2017-11-22 | 2018-04-24 | 青岛农业大学 | U-18496 is being prepared for preventing by the purposes in the fungicide of the microbial plant disease of pathogenic |
| CN107950540B (en) * | 2017-11-22 | 2020-05-26 | 青岛农业大学 | Application of 5-azacytidine in preparation of bactericide for preventing and treating plant diseases caused by phytopathogens |
| CN108901580A (en) * | 2018-07-25 | 2018-11-30 | 河南科技大学 | A method of promoting eary maturity of grape |
| CN110042120A (en) * | 2019-05-07 | 2019-07-23 | 沈阳农业大学 | The method for improving soybean resistance inducing clumping bud efficiency using 5- aza-cytidine |
| CN111034482A (en) * | 2020-01-15 | 2020-04-21 | 河南大学 | Method for treating chrysanthemum cutting to promote chrysanthemum to bloom in advance |
| CN115735772A (en) * | 2022-12-01 | 2023-03-07 | 青岛农业大学 | Application of 5-AzAC in rooting culture of apple rootstock tissues or sugar-free rooting culture |
| CN115735772B (en) * | 2022-12-01 | 2023-08-29 | 青岛农业大学 | Application of 5-AzaC in apple stock tissue rooting culture or sugarless rooting culture |
| CN117204220A (en) * | 2023-09-13 | 2023-12-12 | 扬州大学 | Treatment method for improving seed production rate of cucumber seed melon |
| CN117204220B (en) * | 2023-09-13 | 2024-06-11 | 扬州大学 | Treatment method for improving seed production rate of cucumber seed melon |
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