CN102197778A - Micropropagation method for castanopsis hystrix twigs - Google Patents
Micropropagation method for castanopsis hystrix twigs Download PDFInfo
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- CN102197778A CN102197778A CN2011101356310A CN201110135631A CN102197778A CN 102197778 A CN102197778 A CN 102197778A CN 2011101356310 A CN2011101356310 A CN 2011101356310A CN 201110135631 A CN201110135631 A CN 201110135631A CN 102197778 A CN102197778 A CN 102197778A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/40—Afforestation or reforestation
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Abstract
The invention relates to a micropropagation method for castanopsis hystrix twigs. The micropropagation method comprises the following steps: conventionally accelerating germination of high-quality seeds of castanopsis hystrix of more than 20 years old, and transplanting into a container cup for seedling cultivation; transplanting seedlings capable of receiving complete illumination onto an elevated seedling culturing bed containing base materials in a plastic greenhouse for seedling cultivation; cutting tops to promote sprouting, collecting lateral shoots, and transplanting into a container cup containing base materials in a cuttage way; and placing the container cup on the elevated seedling culturing bed in the plastic greenhouse for seedling cultivation to obtain container cuttage seedlings of castanopsis hystrix. By adopting the elevated seedling culturing bed and the seedling culturing base materials taking yellow soil as the main part to plant parent seedlings, water and nutrition conditions can be favorably controlled, and high-quality cut shoots can be cultured; meanwhile, when an air temperature and humidity regulator of the plastic greenhouse is used to strictly control the relative humidity and temperature of the air in the seedling culturing bed, the cuttage survival rate of castanopsis hystrix reaches more than 90-97%, and each parent seedling can produce 20-40 shoots. The method for culturing cuttage seedlings of castanopsis hystrix is convenient to operate, has low production cost, and can be used for rapidly culturing a large amount of high-quality asexual seedlings for forest planting, thereby having favorable market application prospects.
Description
Technical field
The invention belongs to field of plant growing technology, be specifically related to a kind of red awl spray micro-propagation method.
Background technology
Red awl (Castanopsis hystrix) is a Fagaceae Castanopsis aiphyllium, the south that economize in Guangxi, Guangdong, Hainan, Yunnan, Guizhou and Hunan, Jiangxi, Fujian etc., and all there is distribution the Motuo County in Tibet, and also there are distribution in Vietnam, Burma, India.The massif area of central distribution district below south Guangxi height above sea level 700m.Its growth is very fast, and rudiment power is strong, is thick with leaves, more anti-concealment, mixing natural disposition can be good, for knob, south China and pine, China fir or other broad-leaved trees mix hand over afforestation on select seeds, be that the important broad-leaved of one's native land in South China is precious with material and efficient multi-purpose seeds.Red awl material is good, the hard corrosion resistant of timber, and heartwood is big, maroon, sapwood pale red, color and luster and beautiful texture, few distortion, dry back cracking is little, the material discharge coefficient to reach 2370, belongs to the high-quality assortment, tangent plane is smooth, color and luster is ruddy attractive in appearance, and gumminess and paint are functional, are high-quality materials such as noble furniture, shipbuilding, vehicle, technology engraving, construction and decoration; Branch, flaw-piece, broken material, wood shavings etc. are the good materials of staple fibre, paper pulp, fiber board, particle board etc., also are the quality raw materials of cultivating edible mushroom; Bark and acorn-cup contain tannin 10～15%, can make the tannin extract raw material.
In recent years, province such as Guangxi, Fujian (autonomous region) forestry science and technology worker has carried out a large amount of research to aspects such as the investigation of red awl natural forest, population structure, fine-variety breeding, high-yield cultivation techniques.Guangxi Forest Scientific Research Academy has selected a collection of good geographical provenances such as Pubei, Guangxi, Bobai, draws seed collecting seed production stand 500hm in counties and districts such as Pubei, Guangxi, Bobai and Donglans
2But, because everybody is not enough to the protection of indigenous tree species consciousness, adding the driving of economic interests, the area of red awl natural forest falls sharply day by day, and red awl is solid that the biennial bearing phenomenon arranged, and almost No kernels or seeds are gathered, as in a year of scarcity in off year, is unfavorable for the preservation and the development of red awl.
At present, be confined to for the cottage propagation investigative technique of red awl that conventional elite stand is cut down a coppice shoot cuttage and the field planting maternal plant is short sprouts cuttage, be subject to the meteorological condition restriction, the plugged ear quality is low, and the cutting survival instability can not satisfy and use seedling in a large number in the production.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, a kind of red awl spray micro-propagation method is provided.
Purpose of the present invention is achieved by the following technical programs:
Red awl spray micro-propagation method, comprise female Miao Peiyu, the fringe bar is gathered, cuttage of fringe bar and slotting seedling are cultivated operation, it is characterized in that: after adopting the conventional vernalization of the above good red awl seed of life in 20 years, transplant and in container cup, cultivate seedling, the sprigging that to accept complete illumination is equipped with in the plastic tunnel on the overhead bed of growing seedlings of matrix and is grown seedlings, cutting the top urgees to sprout, gather the cuttage of side fringe in the container cup that matrix is housed, place on the overhead bed of growing seedlings in the plastic tunnel and grow seedlings, and, obtain red awl container cutting propagation seedling with the air themperature and the relative moisture of booth air temperature and humidity regulator control cutting bed.
Red awl container spray micro-propagation method, adopt following steps:
(1) seed collection: select more than the life in 20 years, perfectly straight, crown well-balanced, the excellent strain of red awl that robust growth does not have damage by disease and insect of trunk annual 11～December, collecting fruit, it is dry and cracked to kind of shell to dry in the air in indoor the moon, comes off until seed.
(2) female Miao Peiyu:
1. sowing: place clear water to soak in seed, change water every day, it is clean with flushing with clean water to pull seed out after 4～7 days, be sowed on the wet husky bed clean in the shading shed, press gently with plank that seed is little to sink in the sand, again with the husky seed that covers, be advisable last sprinkle clear water to lose seed.
2. sprigging: take grow root and bud but bud seedling that true leaf does not launch as yet from husky bed, and cut off main root with scraper from distance seed 1.5～3cm, be transplanted to then matrix is housed in the shading shed, specification is in the container cup of 8～12 * 10～14cm, transplant the back that finishes and drench normal root water.
3. seedling is managed and protected: cover the sealing seedbed with film, after 4 o'clock of afternoon, raised film ventilation, trickle, 800～1000 times of carbendazim of spray or thiophanate methyl sterilization every 7 days, again cover the sealing seedbed with film behind the 1h, take film away after 50～70 days, execute one time 0.3～0.5% compound rich water liquid every pouring in 14 days, in shading shed, continue to cultivate after 70～90 days, can draw shading screen in, accept complete illumination.
4. the hardening of being completely cured: the mobile containers cup, cut off and prick underground root, and after 20 days control water hardening.
(3) female seed is planted: select trunk straight from the seedling that refines, height of seedling 20～40cm in the strong sprout of ground stem 0.15～0.35cm, is transplanted to the overhead bed plantation of growing seedlings that seedling medium is housed in the plastic tunnel after peeling container cup off.
(4) female seedling is managed and protected: draw film when it is fine in, allow maternal plant accept complete illumination, then draw back the film rain cover during continuous rainfall, use booth air temperature and humidity regulator to keep the interior temperature of plastic tunnel at 15～35 ℃, relative air humidity is 50～70%, drenched every 14 days and to execute one time 0.5～1.0% compound rich water liquid, begin to gather then to change into behind the fringe bar adopting to drench behind the fringe at every turn and execute one time 0.3% compound rich water liquid.
(5) cutting the top urgees to sprout: otch only keeps 1～2 side shoot near the top apart from eye 2～4cm place, the top, and all the other side shoots are pressed close to trunk and wiped out.
(6) the fringe bar is gathered: as the long 10～25cm of branch, can gather the fringe bar when branch stem base portion is semi-lignified and be used for cuttage.
(7) fringe bar cuttage: wipe out the lateral leaflets on the fringe bar, again cut a fresh wedge-formed incision in fringe bar base portion, 30 one tying up, vertical pendulum is placed in the basin that fills clear water, the clear water degree of depth 4～6cm, be made into 400～600ppm solution with special-purpose root-inducing powder, add talcum powder and stir into pulpous state, plugged ear is taken out from clear water, after drying water, following section speed is dipped in and is inserted in behind the root-inducing powder slurry in the container cup that 8～12 * 10～14cm is equipped with matrix, and container cup is placed on the overhead bed of growing seedlings in the plastic tunnel, pouring normal root water cuttage is finished after.
(8) inserting seedling cultivates: in cuttage in 5～October, must shade with shading screen, the cuttage in April then must be put shading screen away in November to next year, allow the sunshine shining directly cutting bed, and use booth air temperature and humidity regulator to make the interior temperature of cutting bed remain on 20～35 ℃, relative air humidity remained on 85～98%, opened the film ventilation every 7 days, checked the cuttage rooting situation, to fall leaves, deadwood, diseased plants etc. are picked up totally, spray 800～1000 times of carbendazim or thiophanate methyl sterilization then, reseal film behind the 0.5h, treat that plugged ear takes root stable, when reaching 20～35cm, must shift out container seedling from plastic tunnel height of seedling, shade with shading screen, and before 11 of mornings, open shading screen later at 4 in afternoon, allow seedling adapt to illumination gradually, can put shading screen away after 20 days, garden afforestation is prepared out in illumination fully.
The described overhead bed of growing seedlings is highly to be 0.8～1.2m, and width is 0.8～1.2m, and discharge outlet is established to 0.5～2 ° of lopsidedness in the bottom, seedbed, and the matrix degree of depth in the seedbed is 30～40cm.
The described overhead bed of growing seedlings is provided with several layers, can regulate and reduce to ground or lift away from ground.
Described seedling medium is yellow soil 80～90%, baked wheaten cake soil 19.8～9.8%, calcium magnesium phosphorus 0.2% or yellow soil 70～90%, peat soil 8～28%, paulownia bran 2%.
Described cutting medium is a yellow soil 50～80% and through the coconut palm chaff 20～50% of 1 year stack retting.
The described overhead bed plantation seeding row spacing of growing seedlings is 10～30cm * 20～40cm.
Described to cut the short time of sprouting of top be after female seed is planted 6～12 months, and the position is apart from stromal surface 10～25cm place.
It is the thick 0.15～0.30cm of fringe bar base portion that described fringe bar collection requires, and gathers the fringe bar when a pair of blade in top has changeed green.
The present invention has the following advantages with respect to prior art:
1. red awl container spray micro-propagation method of the present invention is 10～30cm * 20～40cm with the plant line-spacing plants the female seed of cuttage on the overhead bed of growing seedlings in plastic tunnel, and can control the degree of lignification of female Miao Shuifen and plugged ear preferably.
2. red awl container spray micro-propagation method of the present invention, with the main matrix of yellow soil as female Miao Peiyu, soil nutrient content is low, the artificial growth potential of very easily controlling female seedling, gained fringe bar is sturdy, side shoot is few, and weeds are few simultaneously, grass roots is shallow, removes easily, has reduced the influence of weeding to female Miao Mengzhi.
3. red awl container spray micro-propagation method of the present invention uses booth air temperature and humidity regulator, has shortened the cuttage root-taking time, cuttage survival rate brings up to 90～97%, simultaneously also shortened the plugged ear acquisition interval time, improved breeding rate, the female seedling of every strain can be adopted 20～40 of fringe bars every year.
4. red awl container spray micro-propagation method of the present invention, but annual all cuttages have prolonged cutting time, are applicable to large tracts of land afforestation.
Specific embodiment
By specific embodiment given below, can further be well understood to the present invention, but they not limitation of the invention.
Embodiment 1
Annual 11～December, select more than lifes in 20 years, perfectly straight, crown well-balanced, the excellent strain of red awl that robust growth does not have damage by disease and insect of trunk, collecting fruit, extremely indoor the moon dries in the air dry and crackedly to kind of shell, comes off until seed.Place clear water to soak in seed, change water every day, it is clean with flushing with clean water to pull seed out after 7 days, is sowed on the wet husky bed clean in the shading shed, presses gently with plank that seed is little to sink in the sand, with the husky seed that covers, is advisable last sprinkle clear water again to lose seed.March in next year, take grow root and bud but bud seedling that true leaf does not launch as yet from husky bed, and cut off main root with scraper from distance seed 1.5cm, be transplanted to then and be equipped with in the shading shed in the container cup that the matrix specification is 8 * 10cm, transplant the back pouring normal root water that finishes.Cover the sealing seedbed with film, after 4 o'clock of afternoon, raised film ventilation, trickle, 800 times of carbendazim of spray or thiophanate methyl sterilization every 7 days, again cover the sealing seedbed with film behind the 1h, take film away after 50 days, execute one time 0.3% compound rich water liquid every pouring in 14 days, in shading shed, continue to cultivate after 70 days, can draw shading screen in, accept complete illumination.In 9～October, the mobile containers cup cuts off and pricks underground root, and controls the water hardening after 20 days.From the seedling that refines, select October trunk straight, height of seedling 20cm, strong sprout more than the ground stem 0.15cm, be transplanted on the overhead bed of growing seedlings that seedling medium is housed in the plastic tunnel after peeling container cup off, seedling medium is yellow soil 80%, burns soil 19.8% and calcium magnesium phosphorus 0.2%, and the overhead bed height of growing seedlings is at 0.8m, width is 1.0m, there is discharge outlet the bottom, seedbed to 2 ° of lopsidedness, and female seed plant line-spacing is 10 * 20cm.Draw film when it is fine in, allow maternal plant accept complete illumination, then draw back the film rain cover during continuous rainfall, use booth air temperature and humidity regulator to keep in the plastic tunnel temperature in annual April in November to next year at 15～25 ℃, relative air humidity is 50～70%, drenched every 14 days and to execute one time 0.5% compound rich water liquid, begin to gather then to change into behind the fringe bar adopting to drench behind the fringe at every turn and execute one time 0.3% compound rich water liquid.Female seed is planted to cut after 6 months and pushes up short sprouting, and improves breeding rate, cuts off with scraper apart from stromal surface 10m place in female seedling trunk, and otch only keeps 1～2 side shoot at close top apart from eye 2～4cm place, the top, and all the other side shoots are pressed close to trunk and wiped out.As the long 10cm of branch, the thick 0.15cm of base portion, a pair of blade in top has changeed green, can gather the fringe bar when branch stem base portion is semi-lignified and be used for cuttage.Wipe out the lateral leaflets on the fringe bar, again cut a fresh wedge-formed incision in fringe bar base portion, tie up for 30 one, vertical pendulum is placed in the basin that fills clear water, the clear water degree of depth 4～6cm, be made into 400ppm solution with special-purpose root-inducing powder, add talcum powder and stir into pulpous state, plugged ear is taken out from clear water, behind the drying water, following section speed is dipped in and is inserted in behind the root-inducing powder slurry in the container cup that 8 * 10cm is equipped with cutting medium, and cutting medium is a yellow soil 50% and through the coconut palm chaff 50% of 1 year stack retting.Container cup is placed on the interior overhead bed of growing seedlings of plastic tunnel, drenches normal root water after cuttage is finished.In the cuttage in May, must shade with shading screen, and use booth air temperature and humidity regulator to make the interior temperature of cutting bed remain on 25 ℃, relative air humidity remained on 85%, opened the film ventilation every 7 days, checked the cuttage rooting situation, to fall leaves, deadwood, diseased plants etc. are picked up totally, spray 800～1000 times of carbendazim or thiophanate methyl sterilization then, reseal film behind the 0.5h, treat that plugged ear takes root stable, when reaching 25cm, must shift out container seedling from plastic tunnel height of seedling, shade with shading screen, and before 11 of mornings, open shading screen later at 4 in afternoon, allow seedling adapt to illumination gradually, can put shading screen away after 20 days, garden afforestation is prepared out in illumination fully.Cuttage survival rate reaches 90.8%.
Embodiment 2
Annual 11～December, select more than lifes in 20 years, perfectly straight, crown well-balanced, the excellent strain of red awl that robust growth does not have damage by disease and insect of trunk, collecting fruit, extremely indoor the moon dries in the air dry and crackedly to kind of shell, comes off until seed.Place clear water to soak in seed, change water every day, it is clean with flushing with clean water to pull seed out after 7 days, is sowed on the wet husky bed clean in the shading shed, presses gently with plank that seed is little to sink in the sand, with the husky seed that covers, is advisable last sprinkle clear water again to lose seed.April in next year, take grow root and bud but bud seedling that true leaf does not launch as yet from husky bed, and cut off main root with scraper from distance seed 2cm, be transplanted to then and be equipped with in the shading shed in the container cup that the matrix specification is 10 * 12cm, transplant the back pouring normal root water that finishes.Cover the sealing seedbed with film, after 4 o'clock of afternoon, raised film ventilation, trickle, 900 times of carbendazim of spray or thiophanate methyl sterilization every 7 days, again cover the sealing seedbed with film behind the 1h, take film away after 60 days, execute one time 0.4% compound rich water liquid every pouring in 14 days, in shading shed, continue to cultivate after 80 days, can draw shading screen in, accept complete illumination.In 9～October, the mobile containers cup cuts off and pricks underground root, and controls the water hardening after 20 days.From the seedling that refines, select November trunk straight, height of seedling 20cm, strong sprout more than the ground stem 0.25cm, be transplanted on the overhead bed of growing seedlings that seedling medium is housed in the plastic tunnel after peeling container cup off, seedling medium is yellow soil 70%, peat soil 28% and paulownia bran 2%, and the overhead bed height of growing seedlings is at 1.0m, width is 1.2m, there is discharge outlet the bottom, seedbed to 0.6 ° of lopsidedness, and female seed plant line-spacing is 20 * 30cm.Draw film when it is fine in, allow maternal plant accept complete illumination, then draw back the film rain cover during continuous rainfall, use booth air temperature and humidity regulator to keep in the plastic tunnel temperature in annual April in November to next year at 15～25 ℃, relative air humidity is 50～70%, drenched every 14 days and to execute one time 0.8% compound rich water liquid, begin to gather then to change into behind the fringe bar adopting to drench behind the fringe at every turn and execute one time 0.3% compound rich water liquid.Female seed is planted to cut after 8 months and pushes up short sprouting, and improves breeding rate, cuts off with scraper apart from stromal surface 18cm place in female seedling trunk, and otch only keeps 1～2 side shoot at close top apart from eye 2～4cm place, the top, and all the other side shoots are pressed close to trunk and wiped out.As the long 20cm of branch, the thick 0.22cm of base portion, a pair of blade in top has changeed green, can gather the fringe bar when branch stem base portion is semi-lignified and be used for cuttage.Wipe out the lateral leaflets on the fringe bar, again cut a fresh wedge-formed incision in fringe bar base portion, 30 one tying up, vertical pendulum is placed in the basin that fills clear water, the clear water degree of depth 4～6cm, be made into 500ppm solution with special-purpose root-inducing powder, add talcum powder and stir into pulpous state, plugged ear is taken out from clear water, after drying water, following section speed is dipped in and is inserted in behind the root-inducing powder slurry in the container cup that 10 * 12cm is equipped with cutting medium, and cutting medium is a yellow soil 70% and through the coconut palm chaff 30% of 1 year stack retting, container cup is placed on the interior overhead bed of growing seedlings of plastic tunnel, drenches normal root water after cuttage is finished.In the cuttage in August, must shade with shading screen, and use booth air temperature and humidity regulator to make the interior temperature of cutting bed remain on 30 ℃, relative air humidity remained on 90%, opened the film ventilation every 7 days, checked the cuttage rooting situation, to fall leaves, deadwood, diseased plants etc. are picked up totally, spray 800～1000 times of carbendazim or thiophanate methyl sterilization then, reseal film behind the 0.5h, treat that plugged ear takes root stable, when reaching 30cm, must shift out container seedling from plastic tunnel height of seedling, shade with shading screen, and before 11 of mornings, open shading screen later at 4 in afternoon, allow seedling adapt to illumination gradually, can put shading screen away after 20 days, garden afforestation is prepared out in illumination fully.Cuttage survival rate reaches 95.3%.
Embodiment 3
Annual 11～December, select more than lifes in 20 years, perfectly straight, crown well-balanced, the excellent strain of red awl that robust growth does not have damage by disease and insect of trunk, collecting fruit, extremely indoor the moon dries in the air dry and crackedly to kind of shell, comes off until seed.Place clear water to soak in seed, change water every day, it is clean with flushing with clean water to pull seed out after 7 days, is sowed on the wet husky bed clean in the shading shed, presses gently with plank that seed is little to sink in the sand, with the husky seed that covers, is advisable last sprinkle clear water again to lose seed.May in next year, take grow root and bud but bud seedling that true leaf does not launch as yet from husky bed, and cut off main root with scraper from distance seed 3cm, be transplanted to then and be equipped with in the shading shed in the container cup that the matrix specification is 12 * 14cm, transplant the back pouring normal root water that finishes.Cover the sealing seedbed with film, after 4 o'clock of afternoon, raised film ventilation, trickle, 1000 times of carbendazim of spray or thiophanate methyl sterilization every 7 days, again cover the sealing seedbed with film behind the 1h, take film away after 70 days, execute one time 0.5% compound rich water liquid every pouring in 14 days, in shading shed, continue to cultivate after 90 days, can draw shading screen in, accept complete illumination.In 9～October, the mobile containers cup cuts off and pricks underground root, and controls the water hardening after 20 days.From the seedling that refines, select December trunk straight, height of seedling 40cm, strong sprout more than the ground stem 0.35cm, be transplanted on the overhead bed of growing seedlings that seedling medium is housed in the plastic tunnel after peeling container cup off, seedling medium is yellow soil 80%, peat soil 18% and paulownia bran 2%, the overhead bed height 1.2m that grows seedlings, width 1.2m, the bottom, seedbed is provided with discharge outlet to 1.5 ° of lopsidedness, and female seed plant line-spacing is 30 * 40cm.Draw film when it is fine in, allow maternal plant accept complete illumination, then draw back the film rain cover during continuous rainfall, use booth air temperature and humidity regulator to keep in the plastic tunnel temperature in annual April in November to next year at 15～25 ℃, relative air humidity is 50～70%, drenched every 14 days and to execute one time 1.0% compound rich water liquid, begin to gather then to change into behind the fringe bar adopting to drench behind the fringe at every turn and execute one time 0.3% compound rich water liquid.Female seed is planted to cut after 12 months and pushes up short sprouting, and improves breeding rate, cuts off with scraper apart from stromal surface 25cm place in female seedling trunk, and otch only keeps 1～2 side shoot at close top apart from eye 2～4cm place, the top, and all the other side shoots are pressed close to trunk and wiped out.As the long 25cm of branch, the thick 0.30cm of base portion, a pair of blade in top has changeed green, can gather the fringe bar when branch stem base portion is semi-lignified and be used for cuttage.Wipe out the lateral leaflets on the fringe bar, again cut a fresh wedge-formed incision in fringe bar base portion, 30 one tying up, vertical pendulum is placed in the basin that fills clear water, the clear water degree of depth 4～6cm, be made into 600ppm solution with special-purpose root-inducing powder, add talcum powder and stir into pulpous state, plugged ear is taken out from clear water, after drying water, following section speed is dipped in and is inserted in behind the root-inducing powder slurry in the container cup that 12 * 14cm is equipped with cutting medium, and cutting medium is a yellow soil 80% and through the coconut palm chaff 20% of 1 year stack retting, container cup is placed on the interior overhead bed of growing seedlings of plastic tunnel, drenches normal root water after cuttage is finished.In the cuttage in December, put shading screen away, allow the sunshine shining directly cutting bed, and use booth air temperature and humidity regulator to make the interior temperature of cutting bed remain on 35 ℃, relative air humidity remains on 95%, opened the film ventilation every 7 days, check the cuttage rooting situation, will fall leaves, deadwood, diseased plants etc. are picked up totally, spray 800～1000 times of carbendazim or thiophanate methyl sterilization then, 0.5h after reseal film, treat that plugged ear takes root stablely, must shift out container seedling from plastic tunnel when height of seedling reaches 35cm, shade with shading screen, and before 11 of mornings, open shading screen later at 4 in afternoon, allow seedling adapt to illumination gradually, can put shading screen away after 20 days, garden afforestation is prepared out in illumination fully.Cuttage survival rate reaches 97.0%.
Claims (9)
1. red awl spray micro-propagation method, comprise female Miao Peiyu, the fringe bar is gathered, cuttage of fringe bar and slotting seedling are cultivated operation, it is characterized in that: after adopting the conventional vernalization of the above good red awl seed of life in 20 years, transplant and in container cup, cultivate seedling, the sprigging that to accept complete illumination is equipped with on the overhead bed of growing seedlings of matrix in plastic tunnel, cutting the top urgees to sprout, gather the cuttage of side fringe in the container cup that matrix is housed, place on the overhead bed of growing seedlings in the plastic tunnel and grow seedlings, and, obtain red awl container cutting propagation seedling with the air themperature and the relative moisture of booth air temperature and humidity regulator control cutting bed.
2. the described red awl container spray micro-propagation method of root a tree name claim 1 is characterized in that: adopt following steps:
(1) seed collection: select more than the life in 20 years, perfectly straight, crown well-balanced, the excellent strain of red awl that robust growth does not have damage by disease and insect of trunk annual 11～December, collecting fruit, it is dry and cracked to kind of shell to dry in the air in indoor the moon, comes off until seed.
(2) female Miao Peiyu:
1. sowing: place clear water to soak in seed, change water every day, it is clean with flushing with clean water to pull seed out after 4～7 days, be sowed on the wet husky bed clean in the shading shed, press gently with plank that seed is little to sink in the sand, again with the husky seed that covers, be advisable last sprinkle clear water to lose seed.
2. sprigging: take grow root and bud but bud seedling that true leaf does not launch as yet from husky bed, and cut off main root with scraper from distance seed 1.5～3cm, be transplanted to then matrix is housed in the shading shed, specification is in the container cup of 8～12 * 10～14cm, transplant the back that finishes and drench normal root water.
3. seedling is managed and protected: cover the sealing seedbed with film, after 4 o'clock of afternoon, raised film ventilation, trickle, 800～1000 times of carbendazim of spray or thiophanate methyl sterilization every 7 days, again cover the sealing seedbed with film behind the 1h, take film away after 50～70 days, execute one time 0.3～0.5% compound rich water liquid every pouring in 14 days, in shading shed, continue to cultivate after 70～90 days, can draw shading screen in, accept complete illumination.
4. the hardening of being completely cured: the mobile containers cup, cut off and prick underground root, and after 20 days control water hardening.
(3) female seed is planted: select trunk straight from the seedling that refines, height of seedling 20～40cm in the strong sprout of ground stem 0.15～0.35cm, is transplanted to the overhead bed plantation of growing seedlings that seedling medium is housed in the plastic tunnel after peeling container cup off.
(4) female seedling is managed and protected: draw film when it is fine in, allow maternal plant accept complete illumination, the then overhead film rain cover of drawing back during continuous rainfall, use booth air temperature and humidity regulator to keep the interior temperature of plastic tunnel at 15～35 ℃, relative air humidity is 50～70%, drenched every 14 days and to execute one time 0.5～1.0% compound rich water liquid, begin to gather then to change into behind the fringe bar adopting to drench behind the fringe at every turn and execute one time 0.3% compound rich water liquid.
(5) cutting the top urgees to sprout: otch only keeps 1～2 side shoot near the top apart from eye 2～4cm place, the top, and all the other side shoots are pressed close to trunk and wiped out.
(6) the fringe bar is gathered: as the long 10～25cm of branch, can gather the fringe bar when branch stem base portion is semi-lignified and be used for cuttage.
(7) fringe bar cuttage: wipe out the lateral leaflets on the fringe bar, again cut a fresh wedge-formed incision in fringe bar base portion, 30 one tying up, vertical pendulum is placed in the basin that fills clear water, the clear water degree of depth 4～6cm, be made into 400～600ppm solution with special-purpose root-inducing powder, add talcum powder and stir into pulpous state, plugged ear is taken out from clear water, after drying water, following section speed is dipped in and is inserted in behind the root-inducing powder slurry in the container cup that 8～12 * 10～14cm is equipped with matrix, and container cup is placed on the overhead bed of growing seedlings in the plastic tunnel, pouring normal root water cuttage is finished after.
(8) inserting seedling cultivates: in cuttage in 5～October, must shade with shading screen, the cuttage in April then must be put shading screen away in November to next year, allow the sunshine shining directly cutting bed, and use booth air temperature and humidity regulator to make the interior temperature of cutting bed remain on 20～35 ℃, relative air humidity remained on 85～98%, opened the film ventilation every 7 days, checked the cuttage rooting situation, to fall leaves, deadwood, diseased plants etc. are picked up totally, spray 800～1000 times of carbendazim or thiophanate methyl sterilization then, reseal film behind the 0.5h, treat that plugged ear takes root stable, when reaching 20～35cm, must shift out container seedling from plastic tunnel height of seedling, shade with shading screen, and before 11 of mornings, open shading screen later at 4 in afternoon, allow seedling adapt to illumination gradually, can put shading screen away after 20 days, garden afforestation is prepared out in illumination fully.
3. root a tree name claim 1 or 2 described red awl spray micro-propagation methods, it is characterized in that: the described overhead bed of growing seedlings is highly to be 0.8～1.2m, and width is 0.8～1.2m, and the bottom, seedbed is to 0.5～2 ° of lopsidedness, discharge outlet is arranged, and the matrix degree of depth in the seedbed is 30～40cm.
4. the red awl spray micro-propagation method stated of root a tree name claim 1 or 2 or 3, it is characterized in that: the described overhead bed of growing seedlings is provided with several layers, can regulate and reduce to ground or lift away from ground.
5. the described red awl spray micro-propagation method of root a tree name claim 2 is characterized in that: described seedling medium is yellow soil 80～90%, burn soil 19.8～9.8%, calcium magnesium phosphorus 0.2% or yellow soil 70～90%, peat soil 8～28%, paulownia bran 2%.
6. the described red awl spray micro-propagation method of root a tree name claim 2 is characterized in that: described cutting medium is a yellow soil 50～80% and through the coconut palm chaff 20%～50% of 1 year stack retting.
7. the described red awl spray micro-propagation method of root a tree name claim 2 is characterized in that: the described overhead bed plantation plant line-spacing of growing seedlings is 10～30 * 20～40cm.
8. the described red awl spray micro-propagation method of root a tree name claim 2 is characterized in that: it is after female seed is planted 6～12 months that described female seedling is cut the short time of sprouting of top, and the position is apart from stromal surface 10～25cm place.
9. the described red awl spray micro-propagation method of root a tree name claim 2 is characterized in that: it is the thick 0.15～0.30cm of fringe bar base portion that described fringe bar collection requires, and gathers the fringe bar when a pair of blade in top has changeed green.
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