CN103749138A - Cutting propagation method of Michelia macclurei - Google Patents
Cutting propagation method of Michelia macclurei Download PDFInfo
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- CN103749138A CN103749138A CN201410024539.0A CN201410024539A CN103749138A CN 103749138 A CN103749138 A CN 103749138A CN 201410024539 A CN201410024539 A CN 201410024539A CN 103749138 A CN103749138 A CN 103749138A
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Abstract
A cutting propagation method of Michelia macclurei includes the steps: stock plant planting and management, cutting medium sterilization, cutting collection, hormone treatment, and cuttage and post-cuttage management. The method is characterized in that high-quality seedlings are transplanted into medium-containing containers to cultivate cutting stock plants, trunks are cut to promote sprouting, cutting medium is sterilized, high-quality cuttings are treated and placed and cultivated in a sun shed, and high-quality cutting seedlings of Michelia macclurei are obtained. The method has the advantages that a cutting propagation method of Michelia macclurei is provided, cuttings are high in quality and availability, few pest and disease damages occur, survival rate is high, the seedlings can be directly taken out of a nursery for foresting, the effective and reliable method is provided for germplasm resource genetic diversity and germplasm resource conservation for Michelia macclurei, the requirements of China for precious tree species resources are met, and great economic benefit and social benefit are achieved.
Description
Technical field
The invention belongs to plant asexual multiplication technology field, relate to a kind of Michelia macclurei cuttage breeding method.
Background technology
Michelia macclurei (
michelia macclurei Dandy) be Magnoliaceae Michelia aiphyllium, trunk is perfectly straight, and up to 30 meters, tree-like neat appearance, is thick with leaves, emerald green, and flower has fragrance.Michelia macclurei natural distribution in Guangdong, Guangxi, Hainan, weather and the deep acid ground of soil layer of happiness warm and moist, vertical distribution many at height above sea level the hills below 600 meters.Michelia macclurei is drought-enduring resistance to lean, and rudiment power is strong, and growth is rapid, the life-span long (more than a century); Michelia macclurei trunk out-put is high, material is excellent, not yielding, the feature such as do not damaged by worms, and is widely used in the industries such as top-grade furniture, handicraft, plywood and building.Pure or Michelia macclurei mixed forest all have obvious improving effect to timbered soil physicochemical property, and can improve the unit yield of forest; Mixed returning can obviously be improved standing forest microclimate conditions, increases soil fertility, and strengthens Characteristics and Water Conservation Function, is the mixed friendship seeds that a kind of ecology, economical, societal benefits get both, and is the fine tree species that material and greening are used in precious fast-growing native soil.Therefore, set up precious deciduous species forest plantation of one's native land increases Forests Biodiversity and ecosystem service function when high value timber is provided, is the direction of forest development from now on.
Asexual reproduction method is cultivated and two kinds of cuttages in a organized way.Michelia macclurei tissue culture technique research is still few, and tissue to cultivate production cost high, technical requirement is high, fails large-scale development; And Michelia macclurei cottage propagation is at present still in space state.
Summary of the invention
The object of the invention is, for Michelia macclurei cottage propagation blank, to aim to provide a kind of Michelia macclurei cuttage breeding method.
The object of the invention is to be achieved through the following technical solutions:
A kind of Michelia macclurei cuttage breeding method, comprise maternal plant plantation and management, cutting medium and sterilization, collection cuttings, HORMONE TREATMENT, cuttage and insert rear management operation, it is characterized in that: high quality seedling is transplanted to plantation in the container cup that installs matrix and adopt fringe maternal plant, cutting strunk is short to be sprouted, after cutting medium sterilization, select high-quality fringe bar after treatment, be placed in cool canopy and cultivate, obtain Michelia macclurei high-quality cuttage seeding; Main operational steps is:
(1) maternal plant plantation and management: February～March, high quality seedling is transplanted to plantation in the container cup that installs matrix and adopted fringe maternal plant, by 1 year of high 30 cm～40 cm, leading thread 0.4 cm～0.6 cm raw seedling growth, in the percentage by weight of 30 cm × 30 cm, be in the yellow soil of 100 % or the container of red heart soil matrix matter, drench sufficient normal root water, look during this time the water regime trickle of the matrix in weather condition and container, spray routinely weekly bacterium, insecticide control damage by disease and insect;
(2) cutting strunk is short sprouts: maternal plant plantation was used secateurs cutting strunk after 30 days, stayed dry high 15 cm～20 cm, remained with 1 pair～2 pairs blades.
(3) cuttings collection and fringe processed: with secateurs collection high-quality fringe bar and cut fringe bar is pruned by profit.Described collection cuttings gathers semi-lignified band axillalry bud, does not take out branch slightly in fine day annual April～June, on the opposite of bud, cuts angle, retains long 5 cm～10 cm of fringe bar.
(4) HORMONE TREATMENT: cuttings is placed in to the root-inducing promoter preparing and soaks.Described HORMONE TREATMENT is immersed in cuttings base portion 1 h～2 h in the root sun solution of 200 mg/L～400 mg/L.
(5) cuttage and insert after management: cuttings is inserted in the nutritious bag that yellow soil that percentage by weight is 100 % or red heart soil matrix matter are housed, the cuttage degree of depth is 2 cm～3 cm, after cuttage, drench normal root water, with film, covering surrounding seals, add the shady net of lid layer 6 pin black, above Qian Cha Kui face, build the cloudy canopy of 6 pin black that 2 m～3 m are high, in cutting bed, air humidity is controlled at 85 %～90 %; Seedling management is ventilative 1 time of trickle weekly, drenches 500 times～800 times of carbendazol wettable powders that to execute with percentage by weight be 50% spray and replace sterilization with 500 times～800 times liquid of fenaminosulf wetting powder that percentage by weight is 70 %; Insert latter 1 month, the potassium dihydrogen phosphate solution that to spray weekly 1 percentage by weight be 0.4 %～0.6 %, sprays 4 times continuously; After Michelia macclurei cuttage rooting is stable, every 10 days, drench and execute Russia or the Shi Ma board composite fertilizer liquid manure that percentage by weight is 0.5 %～0.6 %, plastic film, shady net are raised, retaining cool canopy continues to shade, before 11 of mornings, pack up shading screen at 4 in afternoon later and adapt to gradually illumination by seedling, after 20 days, remove cool canopy, illumination completely, prepares out garden afforestation.
Above-described yellow soil or red heart soil matrix matter is first by the liquor potassic permanganate that percentage by weight the is 0.5 %～0.8 % matrix of drenching, then sprays with 500 times～800 times liquid of carbendazol wettable powder that percentage by weight is 50 %.
The present invention has the following advantages with respect to existing technology:
1, a kind of Michelia macclurei cuttage breeding method of the present invention, solve Michelia macclurei clone seedling and cultivated technical problem, be easy to control maternal plant growth potential, improve cuttings quality and availability, for a kind of effective, reliable method that provides is provided for Michelia macclurei Genetic Diversity of Germplasm, germ plasm resource.
2, a kind of Michelia macclurei cuttage breeding method of the present invention, cuttings quality and availability are high, can effectively control growth of cuttage seedlings phase damage by disease and insect few, and cuttage survival rate is high.
3, a kind of Michelia macclurei cuttage breeding method of the present invention, can shorten rootage duration, improves cuttage survival rate, and nursery stock can directly go out garden afforestation, has met the demand of China to rare tree resource.
Embodiment
By specific embodiment given below, can further be well understood to the present invention, but they not limitation of the invention.
embodiment 1
Select 1 year raw seedling of height of seedling 30 cm, leading thread 0.4 cm, February, being planted in percentage by weight is that the specification that the red heart soil of 100 % is matrix is in the container of 30 cm × 30 cm, drench sufficient normal root water, look during this time the water regime trickle of the matrix in weather condition and container, spray routinely weekly bacterium, insecticide control damage by disease and insect.Plant after 30 days, select fine day secateurs to carry out cutting strunk and urge to sprout, it is level and smooth that clip is wanted, and retains dry high 15 cm, retains 1 pair of blade, sprays bactericidal agent and trickle moisturizing.April cuttage the previous day, by percentage by weight is housed, be 100% red heart soil matrix matter, specification is that the black nutritive bag percentage by weight of 10 cm × 14 cm is the liquor potassic permanganate of the 0.5 % matrix of drenching, and after 4 hours, sprays stand-by again with 500 times of liquid of carbendazol wettable powder that percentage by weight is 50 %.Select semi-lignified band axillalry bud, do not take out branch slightly as cuttings, by profit, cut on axillalry bud opposite and cut angle, retain long 5 cm of fringe bar, clear water moisturizing, treat that whole cuttings have pruned, with 20/bundle, cuttings base portion is invaded to bubble and after 2 h, insert in the middle of nutritious bag in root sun root-growing agent 200 mg/L solution, the cuttage degree of depth 2 cm, drench normal root water.Cuttage furrow face dowel arch, with film covering, surrounding dirt seal, adds a cover the shady nets of 6 pin black of 1 layer, on cuttage furrow face, is building the 6 pin black cool canopies that 1 floor height is 2 m～3 m, and in cutting bed, air humidity is controlled at 85 %～90 %.After inserting, open weekly trickle ventilative 1 time, with 500 times of liquid of fenaminosulf wetting powder that percentage by weight is 70 %, drench 800 times of carbendazol wettable powders that to execute with percentage by weight be 50% and spray and replace sterilization.Insert latter 1 month, the potassium dihydrogen phosphate solution that to spray weekly 1 percentage by weight be 0.4 %, sprays 4 times continuously.After Michelia macclurei cuttage rooting is stable, every 10 days, drench and execute Russia or the Shi Ma board composite fertilizer liquid manure that percentage by weight is 0.5 %～0.6 %, plastic film, shady net are raised, retaining cool canopy continues to shade, before 11 of mornings, pack up shading screen at 4 in afternoon later and adapt to gradually illumination by seedling, after 20 days, remove cool canopy, illumination completely, prepares out garden afforestation.
embodiment 2
Select 1 year raw seedling of height of seedling 35 cm, leading thread 0.5 cm, February, being planted in percentage by weight is that the specification that the yellow soil of 100 % is matrix is in the container of 30 cm × 30 cm, drench sufficient normal root water, look during this time the water regime trickle of the matrix in weather condition and container, spray weekly bacterium, insecticide control damage by disease and insect.Plant after 30 days, select fine day secateurs to carry out cutting strunk and urge to sprout, it is level and smooth that clip is wanted, and retains dry high 20 cm, retains 1 pair～2 pairs blades, sprays bactericidal agent and trickle moisturizing.May, cuttage will be equipped with the yellow soil matrix that percentage by weight is 100 % the previous day, specification is that the black nutritive bag percentage by weight of 10 cm × 14 cm is the liquor potassic permanganate of the 0.6 % matrix of drenching, and after 4 hours, sprays stand-by again with 800 times of liquid of carbendazol wettable powder that percentage by weight is 50 %.Select semi-lignified band axillalry bud, do not take out branch slightly as cuttings, by profit, cut on axillalry bud opposite and cut angle, retain long 8 cm of fringe bar, clear water moisturizing, treat that whole cuttings have pruned, with 20/bundle, cuttings base portion is invaded to bubble and after 2 h, insert in the middle of nutritious bag in root sun root-growing agent 300 mg/L solution, the cuttage degree of depth 2 cm, drench normal root water.Cuttage furrow face dowel arch, with film covering, surrounding dirt seal, adds a cover the shady nets of 6 pin black of 1 layer, on cuttage furrow face, is building the 6 pin black cool canopies that 1 floor height is 2 m～3 m, and in cutting bed, air humidity is controlled at 85～90 %.After inserting, open weekly trickle ventilative 1 time, with 800 times of liquid of fenaminosulf wetting powder that percentage by weight is 70 %, drench 500 times of carbendazol wettable powders that to execute with percentage by weight be 50 % and spray and replace sterilization.Insert latter 1 month, the potassium dihydrogen phosphate solution that to spray weekly 1 percentage by weight be 0.5 %, sprays 4 times continuously.After Michelia macclurei cuttage rooting is stable, every 12 days, drench and execute Russia or the Shi Ma board composite fertilizer liquid manure that percentage by weight is 0.7 %～0.8 %, plastic film, shady net are raised, retaining cool canopy continues to shade, before 11 of mornings, pack up shading screen at 4 in afternoon later and adapt to gradually illumination by seedling, after 20 days, remove cool canopy, illumination completely, prepares out garden afforestation.
embodiment 3
Select 1 year raw seedling of height of seedling 40 cm, leading thread 0.6 cm, March, being planted in percentage by weight is that the specification that the yellow soil of 100 % is matrix is in the container of 30 cm × 30 cm, drench sufficient normal root water, look during this time the water regime trickle of the matrix in weather condition and container, spray weekly bacterium, insecticide control damage by disease and insect.Plant after 30 days, select fine day secateurs to carry out cutting strunk and urge to sprout, it is level and smooth that clip is wanted, and retains dry high 20 cm, retains 2 pairs of blades, sprays bactericidal agent and trickle moisturizing.June, cuttage will be equipped with the yellow soil matrix that percentage by weight is 100 % the previous day, specification is liquor potassic permanganate that the black nutritive bag percentage by weight of 10 cm × 14 cm is 0.8% matrix of drenching, and after 4 hours, sprays stand-by again with 800 times of liquid of carbendazol wettable powder that percentage by weight is 50 %.Select semi-lignified band axillalry bud, do not take out branch slightly as cuttings, by profit, cut on axillalry bud opposite and cut angle, retain long 10 cm of fringe bar, clear water moisturizing, treat that whole cuttings have pruned, with 20/bundle, cuttings base portion is invaded to bubble and after 1h, insert in the middle of nutritious bag in root sun root-growing agent 400 mg/L solution, the cuttage degree of depth 3 cm, drench normal root water.Cuttage furrow face dowel arch, with film covering, surrounding dirt seal, adds a cover the shady nets of 6 pin black of 1 layer, on cuttage furrow face, is building the 6 pin black cool canopies that 1 floor height is 2 m～3 m, and in cutting bed, air humidity is controlled at 85 %～90 %.After inserting, open weekly trickle ventilative 1 time, with 800 times of liquid of fenaminosulf wetting powder that percentage by weight is 70 %, drench 800 times of carbendazol wettable powders that to execute with percentage by weight be 50 % and spray and replace sterilization.Insert latter 1 month, the potassium dihydrogen phosphate solution that to spray weekly 1 percentage by weight be 0.6 %, sprays 4 times continuously.After Michelia macclurei cuttage rooting is stable, every 15 days, drench and execute Russia or the Shi Ma board composite fertilizer liquid manure that percentage by weight is 0.8 %～1.0 %, plastic film, shady net are raised, retaining cool canopy continues to shade, before 11 of mornings, pack up shading screen at 4 in afternoon later and adapt to gradually illumination by seedling, after 20 days, remove cool canopy, illumination completely, prepares out garden afforestation.
Claims (6)
1. a Michelia macclurei cuttage breeding method, comprise maternal plant plantation and management, cutting medium and sterilization, collection cuttings, HORMONE TREATMENT, cuttage and insert rear management operation, it is characterized in that: high quality seedling is transplanted in the container cup that installs matrix and cultivated and adopt fringe maternal plant, cutting strunk is short to be sprouted, after cutting medium sterilization, select high-quality fringe bar after treatment, be placed in cool canopy and cultivate, obtain Michelia macclurei high-quality cuttage seeding.
2. a Ju a kind of Michelia macclurei cuttage breeding method claimed in claim 1, it is characterized in that: described maternal plant is planted and manages February～March, high quality seedling to be transplanted in the container cup that installs matrix and plants and adopt fringe maternal plant, by 1 year of high 30 cm～40 cm, leading thread 0.4 cm～0.6 cm raw seedling growth, in the percentage by weight of 30 cm × 30 cm, be in the yellow soil of 100 % or the container of red heart soil matrix matter, drench sufficient normal root water, look during this time the water regime trickle of the matrix in weather condition and container, spray routinely weekly bacterium, insecticide control damage by disease and insect; After 30 days, cutting strunk is short sprouts, and stays dry high 15 cm～20 cm, remains with 1 pair～2 pairs blades.
3. a Ju a kind of Michelia macclurei cuttage breeding method claimed in claim 1, it is characterized in that: described collection cuttings gathers semi-lignified band axillalry bud, do not take out branch slightly in fine day annual April～June, angle is cut on opposite at bud, retains long 5 cm～10 cm of fringe bar.
4. a Ju a kind of Michelia macclurei cuttage breeding method claimed in claim 1, is characterized in that: described HORMONE TREATMENT is immersed in cuttings base portion 1 h～2 h in the root sun solution of 200 mg/L～400 mg/L.
5. a Ju a kind of Michelia macclurei cuttage breeding method claimed in claim 1, it is characterized in that: described cuttage and insert after management cuttings is inserted in the nutritious bag that yellow soil that percentage by weight is 100 % or red heart soil matrix matter are housed, the cuttage degree of depth is 2 cm～3 cm, after cuttage, drench normal root water, with film, covering surrounding seals, add the shady net of lid layer 6 pin black, build the cloudy canopy of 6 pin black that 2 m～3 m are high above skewer is inserted Kui face, in cutting bed, air humidity is controlled at 85 %～90 %; Seedling management is ventilative 1 time of trickle weekly, drenches 500 times～800 times of carbendazol wettable powders that to execute with percentage by weight be 50 % spray and replace sterilization with 500 times～800 times liquid of fenaminosulf wetting powder that percentage by weight is 70 %; Insert latter 1 month, the potassium dihydrogen phosphate solution that to spray weekly 1 percentage by weight be 0.4 %～0.6 %, sprays 4 times continuously; After Michelia macclurei cuttage rooting is stable, every 10 days, drench and execute Russia or the Shi Ma board composite fertilizer liquid manure that percentage by weight is 0.5 %～0.6 %, plastic film, shady net are raised, retaining cool canopy continues to shade, before 11 of mornings, pack up shading screen at 4 in afternoon later and adapt to gradually illumination by seedling, after 20 days, remove cool canopy, illumination completely, prepares out garden afforestation.
6. a kind of Michelia macclurei cuttage breeding method described in a Ju claim 2 or 5, it is characterized in that: described yellow soil or red heart soil matrix matter is first by the liquor potassic permanganate that percentage by weight the is 0.5 %～0.8 % matrix of drenching, then spray with 500 times～800 times liquid of carbendazol wettable powder that percentage by weight is 50 %.
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Cited By (4)
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| CN104041392A (en) * | 2014-06-13 | 2014-09-17 | 福建师范大学 | Method for cultivating Machilus pauhoi with high cutting seedling planting percent by light-matrix nutrition bags |
| CN104054501A (en) * | 2014-07-15 | 2014-09-24 | 广西壮族自治区林业科学研究院 | Phoebe bournei cutting propagation method |
| CN106386353A (en) * | 2016-09-14 | 2017-02-15 | 广西壮族自治区林业科学研究院 | Seedling growing method of tender branch cutting for keteleeria cyclolepis flous |
| CN112005803A (en) * | 2020-08-17 | 2020-12-01 | 桂林市林业和园林综合服务中心 | Construction method of Machilus ecological landscape forest |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104041392A (en) * | 2014-06-13 | 2014-09-17 | 福建师范大学 | Method for cultivating Machilus pauhoi with high cutting seedling planting percent by light-matrix nutrition bags |
| CN104041392B (en) * | 2014-06-13 | 2016-03-09 | 福建师范大学 | Chinese bandoline wood Light media nutritious bag cuttage and seedling culture height planting percent breeding method |
| CN104054501A (en) * | 2014-07-15 | 2014-09-24 | 广西壮族自治区林业科学研究院 | Phoebe bournei cutting propagation method |
| CN104054501B (en) * | 2014-07-15 | 2016-08-24 | 广西壮族自治区林业科学研究院 | A kind of Phoebe bournei cuttage breeding method |
| CN106386353A (en) * | 2016-09-14 | 2017-02-15 | 广西壮族自治区林业科学研究院 | Seedling growing method of tender branch cutting for keteleeria cyclolepis flous |
| CN112005803A (en) * | 2020-08-17 | 2020-12-01 | 桂林市林业和园林综合服务中心 | Construction method of Machilus ecological landscape forest |
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