Background technology
" blue maiden " Chinese ilex (Ilex × meserveae S.Y.Hu. ' Blue Girl ') belongs to Aquifoliaceae (Aquifoliaceae) Ilex (Ilex) plant.Gardening Hybrid is (for outstanding kind name, at " blue maiden " the upper quotation marks that add, below roughly the same), produced by I.rugosa ' Long Island ' × I.aquifolium ' Lawrence White ' hybridization, breeding people is K.K.Meserve., this kind individual plant is all female plant, and another kind " blue young boy " Chinese ilex is all staminiferous plant.Evergreen shrubs, plant height 1-3 rice, the short and thin fine hair that branch tool is sparse, blade olive-green, avette, slightly bending, long 2.5-3.5 centimetre, wide is 1.3-1.8 centimetre, base portion circle, most advanced and sophisticated sharp point, 4-6 piece little evagination spine of leaf margin tool, long 5 millimeters of petiole, stem lavender, inflorescence armpit is raw, floral white, fruit ruby look, spherical, footpath 6-8 millimeter, 4 pieces of pyrenes, pedicel pale red, long 6 millimeters, the florescence 4-6 month, the fruit phase 10-12 month.Like warmly, moistening, the environment that light is good, requires fertile soil, and draining is good, be advisable to be rich in organic sand loam, growth season abundant watering, fertilizing, in order to its well-grown.These seeds are suitable for flower garden greening, can make hedgerow, landscape tree.In addition, this kind is undershrub, and resistance to pruning can be used as potted landscape and uses.
At present this kind seedling quantity is considerably less at home, and correlative study shows that the seed germination of " blue maiden " Chinese ilex is more difficult, and therefore " blue maiden " Chinese ilex resource lacks very much, and application is also " have city out of stock ".
Cottage propagation is the first-selected approach of current domestic solution nursery stock production.Have been reported the research of Holly being carried out to cottage propagation, but carry out the cuttage of Holly there are no report research and utilization horse liver soil.As Huanghai Sea swallow utilizes perlite and the peat soil matrix of 3: 1, Central China Chinese ilex is carried out to cuttage (seeing northern gardening o. 11th in 2010), Qian Yongqiu utilizes perlite and the peat soil matrix of 1: 3 to carry out cuttage (seeing Agriculture of Anhui circular, the 21st phase in 2008) to purplefruit holly leaf.Cuttage matrix used requires harsher, mostly is peat soil, perlite and so on, and these matrix exist watering property too large, is loosely difficult for the shortcomings such as solid root, extensive cuttage cost be relatively large.Suit measures to local conditions, the method for cost-saving ground large area industrialization cuttage " blue maiden " Chinese ilex yet there are no report.Horse liver soil is the distinctive a kind of soil in THE LOWER YANGTZE VALLEY area, and Sha Xingqiang dryly can consolidate root, wet can water conservation, but water permeable ability is strong, is most appropriate to the moisture control of cuttings in front of raw.
In sum, utilizing horse liver soil cuttage Fast-propagation " blue maiden " Chinese ilex is one of important channel solving this Tree Species Resources shortage, and exploitation and the utilization and extention of " blue maiden " Chinese ilex are all had to very important meaning.
Summary of the invention
The technical problem that the present invention mainly solves is openly to utilize the method for quickly breeding of horse liver soil simple cuttage " blue maiden " Chinese ilex.
Adopt following technical scheme for solving this technology:
A) matrix treatments: after horse liver soil is pulverized, sneak into loess.According to the ratios of 4: 1, horse liver soil and loess are mixed, pour in the cuttage pond having puzzled, cuttage pond specification is width 2m, height 0.3m, and length is determined according to cuttage quantity.Then, carbendazim is admixed in soil, usage ratio is 4g/m
2.
B) cuttings processing: early July, choose healthy and strong without damage by disease and insect branch as cuttings.The truncation of cuttings lower cut, upper cut are cut sth. askew, otch level and smooth, crack-free; Length is 10-15cm; Cuttings lower cut place is with the adventitious root primordia of hiding (being internode side shoot base portion, indefinite bud base portion or petiole base); Cuttings retains 1 pair of blade, and cuts off a half vane, and need have complete full lateral bud.Cuttings is with adopting with cuttage simultaneously.
C) HORMONE TREATMENT: use the IBA of 1000mg/kg to soak cuttings hypomere 2min, insert immediately slotting machine, use sprinkling irrigation that matrix is irrigated.
D) full exposure is sprayed and is managed: use Timer Controlling water pump, water spraying time control is: fine day, particularly hot weather, and spray time is 3min/ time, and to reach cooling effect, the cloudy day sprays less or does not spray, and is generally 12min/ time, and the rainy day does not spray.Temperature is 20-28 ℃, relative moisture 80%.
E) fertilizing management: step C) complete after 45d, use nutrient solution spray plant blade face, nutrient composition is: N200mg/L, P33mg/L, K66mg/L, phosphate fertilizer is with NH
4h
2pO
4form adds, and potash fertilizer is with KNO
3form adds, and nitrogenous fertilizer is divided by NH
4h
2pO
4and KNO
3form add outside, (NH for insufficient section
4)
2sO
4supplement.It is 25L that nutrient solution sprays volume.Cuttage 60d and 75d, repeat spraying 1 time.
The beneficial effect of the relative prior art of the present invention is: utilize horse liver soil simple cuttage " blue maiden " Chinese ilex, can obtain fast and effectively a large amount of " blue maiden " Chinese ilex seedlings, solve the problem of " blue maiden " Chinese ilex shortage of resources; By to matrix, cuttings, hormone, Sprinkler Irrigation and survive choosing of rear nutrient formulation, can make " blue maiden " Chinese ilex cuttage survival rate reach more than 90%, survive the rear seedling specification (more than plant height 20cm) that reaches fast, can obtain efficiently " blue maiden " Chinese ilex high quality seedling.
Embodiment
Embodiment mono-
1, prepare in matrix, cuttage pond.Horse liver soil is shone after dry grinding, sneak into loess.According to the ratios of 4: 1 (i.e. 4 go-cart horse liver soil and 1 go-cart loess), horse liver soil and loess are mixed, pour in the cuttage pond having puzzled, cuttage pond specification is the high 2m*5m*0.3m of length and width, and pond mesostroma height is 15cm.Then, 40g carbendazim is admixed in soil.Spray 2h, carbendazim is dissolved in matrix.
2, cuttings processing.On July 1st, 2009, choose healthy and strong lifes in 6 years " blue maiden " the Chinese ilex seedling of 50 strains, clip stalwartness without the current-year branch of damage by disease and insect as cuttings, 1000 of clip cuttings altogether.The truncation of cuttings lower cut, upper cut are cut sth. askew, otch level and smooth, crack-free; Length is 10cm; Cuttings lower cut place should be with the adventitious root primordia of hiding (being internode side shoot base portion, indefinite bud base portion or petiole base); Cuttings retains 1 pair of blade, and cuts off a half vane, and has complete full lateral bud.After cuttings is ready to, use the IBA of 1000mg/kg to soak cuttings hypomere 2min, insert immediately slotting machine, insertion density is 10cm*10cm.Use and spray 1h, matrix is irrigated to normal root.
3, full exposure spraying management: use Timer Controlling water pump, water spraying time control is: fine day, high temperature (more than 28 ℃) weather, spray time is 3min/ time, sprays 10h every day; At the cloudy day, spray time is 12min/ time, sprays 8h every day; Rainy day does not spray.Cuttings surface temperature control is being 20-28 ℃, relative moisture 80%.
4,15d after cuttage, cuttings base portion starts the callus of growing.25d, Calli Differentiation, starts to take root, 35d, rooting rate reaches 94.59%, the long 6.14cm that on average reaches of root.
5, fertilizing management: 45d after cuttage, use nutrient solution to spray plant blade face, nutrient composition is: N200mg/L, P33mg/L, K66mg/L, phosphate fertilizer is with NH
4h
2pO
4form adds, and potash fertilizer is with KNO
3form adds, and nitrogenous fertilizer is divided by NH
4h
2pO
4and KNO
3form add outside, (NH for insufficient section
4)
2sO
4supplement.Use sprayer to carry out Foliar Spraying, volume is 25L.Cuttage 60d and 75d, repeat spraying 1 time.
6,70d after cuttage, cuttage seeding is extracted the tender tip out, 90d after cuttage, tender tip exhibition leaf is complete, and plant height on average reaches 20.21cm, the long average out to 13.34cm of root, the radical more than long 10cm of root on average reaches 11.
Embodiment bis-
1, prepare in matrix, cuttage pond.Horse liver soil is shone after dry grinding, sneak into loess.According to the ratios of 4: 1 (i.e. 4 go-cart horse liver soil and 1 go-cart loess), horse liver soil and loess are mixed, pour in the cuttage pond having puzzled, cuttage pond specification is the high 2m*10m*0.3m of length and width, and pond mesostroma height is 15cm.Then, 80g carbendazim is admixed in soil.Spray 2h, carbendazim is dissolved in matrix.
2, cuttings processing.On July 10th, 2010, choose healthy and strong lifes in 6 years " blue maiden " the Chinese ilex seedling of 100 strains, clip stalwartness without the current-year branch of damage by disease and insect as cuttings, 2000 of clip cuttings altogether.The truncation of cuttings lower cut, upper cut are cut sth. askew, otch level and smooth, crack-free; Length is 15cm; Cuttings lower cut place should be with the adventitious root primordia of hiding (being internode side shoot base portion, indefinite bud base portion or petiole base); Cuttings retains 1 pair of blade, and cuts off a half vane, and has complete full lateral bud.After cuttings is ready to, use the IBA of 1000mg/kg to soak cuttings hypomere 2min, insert immediately slotting machine, insertion density is 10cm*10cm.Use and spray 1h, matrix is irrigated to normal root.
3, full exposure spraying management: use Timer Controlling water pump, water spraying time control is: fine day, high temperature (more than 28 ℃) weather, spray time is 3min/ time, sprays 10h every day; At the cloudy day, spray time is 12min/ time, sprays 8h every day; Rainy day does not spray.Cuttings surface temperature control is being 20-28 ℃, relative moisture 80%.
4,17d after cuttage, cuttings base portion starts the callus of growing.23d, Calli Differentiation, starts to take root, 35d, rooting rate reaches 90.15%, the long 4.75cm that on average reaches of root.
5, fertilizing management: 45d after cuttage, use nutrient solution to spray plant blade face, nutrient composition is: N200mg/L, P33mg/L, K66mg/L, phosphate fertilizer is with NH
4h
2pO
4form adds, and potash fertilizer is with KNO
3form adds, and nitrogenous fertilizer is divided by NH
4h
2pO
4and KNO
3form add outside, (NH for insufficient section
4)
2sO
4supplement.Use sprayer to carry out Foliar Spraying, volume is 25L.Cuttage 60d and 75d, repeat spraying 1 time.
6,68d after cuttage, cuttage seeding is extracted the tender tip out, 90d after cuttage, tender tip exhibition leaf is complete, and plant height on average reaches 20.94cm, the long average out to 12.73cm of root, the radical more than long 10cm of root on average reaches 9.