CN109156185A - A kind of masson pine Water culture method for culturing seedlings - Google Patents
A kind of masson pine Water culture method for culturing seedlings Download PDFInfo
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- CN109156185A CN109156185A CN201811018717.3A CN201811018717A CN109156185A CN 109156185 A CN109156185 A CN 109156185A CN 201811018717 A CN201811018717 A CN 201811018717A CN 109156185 A CN109156185 A CN 109156185A
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- cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C5/00—Fertilisers containing other nitrates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Abstract
The present invention provides a kind of masson pine Water culture method for culturing seedlings, including cuttings selects, Pretreatment and Water culture, using masson pine tissue-cultured seedling as mother plant for cutting, acquisition current-year branch is cuttings, Water culture and daily management, the final masson pine cuttage seeding for obtaining raised growth stalwartness are carried out in hydroponic box after carrying out Pretreatment to cuttings.This method can effectively improve the cutting experiment of lignifying branch and the utilization efficiency of branch; masson pine asxualization nursery stock large-scale cultivation efficiency, and strong operability are promoted, it is low in cost; it can be widely applied to production of forestry unit, have broad application prospects and technological achievement Transformation Potential.
Description
Technical field
The invention belongs to asxualization nursery technologies, and in particular to a kind of masson pine Water culture method for culturing seedlings.
Background technique
Masson pine (Pinus massonianaIt Lamb. is) the main reproducting tree species of south China, in Guangxi, Fujian, cloud
There are introducing and planting in 17 provinces and regions in the whole nation such as south, Guizhou, Sichuan, Zhejiang, widely used, are not only excellent construction timber,
Or furthermore excellent papermaking and staple fibre board raw material can also be used to produce the chemical products such as rosin, turpentine oil, in state of China
Particularly important effect is played in people's economic construction.With the growing life requirement of people and timber resource shortage and stone
The non-renewable resources such as oil, which are petered out, waits intensifications of contradictions, and the sustainable developments of ecology, economy and society are increasingly by society
It can pay close attention to.The problems such as based on China's ecological safety, resource security and social safety, masson pine is as the main industrial cut stock in China
It is necessary to greatly develop its artificial forest for tree species and Tree Species as Bio-energy.
Existing masson pine fine germplasm resources are made full use of, tissue-cultured seedling is cultivated as maternal plant, builds cutting orchard, utilize its fringe
Item progress cuttage, propagation in scale asxualization nursery stock are applied to production afforestation, are when preacceleration masson pine afforestation improved variety process
With promotion artificial forest unit area productivity the most effective means.But due to the influence of the natural causes such as weather, in 1 year only
Better effects can be obtained by only carrying out cuttage with spring in the fall, and then effect is poor in temperature higher summer, and cuttage branch holds
Perishable rooting rate is low, and effective cutting time is short, and nursery stock production efficiency is totally lower；In addition, using first lignifying shoot cutting
Effect is good and uses the higher branch cutting effect of degree of lignification poor, in addition in production practice, horse hair pine cuttage fringe bar
It is easy to physiological ageing, most of degree of lignification is higher, thus branch is caused to efficiently use low efficiency, and serious waste of resources is right
Masson pine asxualization nursery stock propagation in scale adversely affects.
Summary of the invention
For lignifying branch in existing Cuttage Seedling-Raising of Masson Pine method cutting experiment is poor, branch efficiently uses low efficiency
The deficiencies of, the present invention provides a kind of masson pine Water culture method for culturing seedlings, this method can effectively improve lignifying branch
The utilization efficiency of cutting experiment and branch promotes masson pine asxualization nursery stock large-scale cultivation efficiency.
The present invention is achieved by the following technical scheme:
A kind of masson pine Water culture method for culturing seedlings, including cuttings selects, Pretreatment and Water culture, with masson pine tissue culture
Seedling is mother plant for cutting, and acquisition current-year branch is cuttings, and water planting skewer is carried out in hydroponic box after carrying out Pretreatment to cuttings
It inserts and daily management, the final masson pine cuttage seeding for obtaining raised growth stalwartness, main operational steps is as follows:
(1) cuttings selects
It is mixed using 1500~1800 times of sterilizing insecticide of mixing 3 days before acquiring cuttings using masson pine tissue-cultured seedling as mother plant for cutting
Heshui solution sprays 1 time mother plant for cutting progress comprehensively；Select 5~10 cm of current growth, 1.5~3.5 mm of diameter, growing way compared with
The branch of good no disease and pests harm is cuttings；
(2) Pretreatment
1. the cuttings that will be collected extracts the needle grown in 3 cm of base portion, being tied 20~30 plants with rubber band is a bundle,
Lower end keeps smooth, and immersing mass concentration rapidly is to carry out at cuttings disinfection in 0.3~0.5% chlorobromide isocyanuric acid aqueous solution
Reason, immersion 3~rinsed well for use with clear water after five minutes, cuttings soaking depth are 3~4 cm；
2. the cuttings after disinfection is immersed promoter to impregnate 30~45 minutes, cuttings soaking depth is 1~2 cm；The promoting root growth
Agent is made of following density component: IAA 200-300 mg/L, ABT1200-300 mg/L, paclobutrazol 10-15 mg/L, VB12
10-20 mg/L, VE 10-20 mg/L, niacin 100-150 mg/L, biotin 50-100 mg/L, KNO3 1200 mg/L、
Mg(NO3)2·6H2O 250 mg/L、H3BO3 20 mg/L、Na2S2O3 273 mg/L；
(3) Water culture
1. cuttage is handled
It is by volume that 4:1 is uniformly mixed into culture solution and falls back and trains in device by promoter and water, the depth of culture solution is
4.5 cm；The fringe bar obtained by step (2) processing is inserted into hydroponic device, it is 1~2 that fringe bar base portion, which is inserted into medium depth,
cm；
It is ventilated, is ventilated 15 minutes within every 2 hours, until cuttings base portion callus using culture solution of the inflator pump timing into hydroponic device
It organizes the formation of or starts to grow the tip of a root；
2. daily management
Culture solution is primary every replacement in 5 days, to remove the impurity in culture solution in time；If should be clear in time it was found that cuttings growing way is bad
It removes；When summer weather sweltering heat strong sunlight, sunshade protection is carried out to hydroponic device with sunshade net in time, while spraying to fringe bar
Mist moisturizing, autumn, winter build plastic canopy radix saposhnikoviae.
Further, mixing sterilizing insecticide described in above-mentioned steps (1) is by 70% Wettable propineb powder, 60% benzene
Ether methyl cyclic-azole wettable powder and 80% metrifonate wettable powder are mixed to get wettable according to the ratio that volume ratio is 1:1:1
Property pulvis.
Further, cuttings described in above-mentioned steps (1) is the masson pine branch of semi-lignified or complete lignifying.
Further, the hydroponic device in above-mentioned steps (3) includes open bubble chamber and foam cover board；The foam cap
Plate is detachably secured to the open-mouth of bubble chamber；The aperture conduct that diameter is 2.0cm is uniformly distributed on the foam cover board
Cutting hole；The bubble chamber bottom is equipped with water inlet, water outlet and inflating port；The inflating port is connect with inflator pump；If
Fringe bar is not fixed easily, and can fill cutting hole with sponge or cotton.
The technical program has the advantages that compared with prior art
1, the present invention is in cuttings selects step, and 3 days before acquiring cuttings, use is with Propineb, difenoconazole and metrifonate
The mixed aqueous solution quantitatively prepared sprays mother plant for cutting, the antibacterial disease prevention ability of the cuttings after improving acquisition, conducive to mentioning
The survival rate of high cuttings.
2, the present invention carries out disinfection to cuttings using chlorobromide isocyanuric acid aqueous solution in Pretreatment step, can be with
Cuttings is effectively avoided to carry germ；Before cuttage with promoter impregnate cuttings can make medical fluid from notch infiltration cuttings internal action in
Nutritional ingredient needed for more plant cells, and supplement cuttings, conducive to the rooting rate for improving cuttings.
3, the culture solution that can be used as Water culture after the promoting root growth dilution agent that the present invention prepares uses, in promoter
IAA、ABT1Cuttings base portion callus can be effectively facilitated with paclobutrazol to be formed, and improve the rooting rate of cuttings；KNO3 、Mg
(NO3)2·6H2O、H3BO3Various nutritional ingredients needed for addition with various vitamins can provide cuttings growth；Promoting simultaneously
The H added in root agent3BO3And Na2S2O3Collective effect can effectively kill the germ in culture solution and inhibit the oxygen of cuttings notch
Change reaction, avoiding water planting environmental nutrient liquid from breeding germ reduces the survival rate of cuttings.
4, the present invention carries out water planting using semi-lignified or complete lignifying branch, can also obtain good effect, water planting
Rooting rate reaches 98.3% or more after 30 days, greatly improves the utilization rate of branch, avoids the waste of resource, improves life
Efficiency is produced, and this method can all carry out asxualization nursery stock large-scale production throughout the year, not limited by season and temperature, extended
Effective cutting time, improves asxualization cultivating seedlings efficiency.
5, the present invention, which combines closely, produces reality, and the practicality and strong operability are low in cost, can be widely applied to woods
Industry production unit has broad application prospects and technological achievement Transformation Potential.
Specific embodiment
The present invention is further illustrated by the following examples, but not as limitation of the present invention.In the following example not
Dated specific experiment condition and method, used technological means are usually conventional hand well-known to those skilled in the art
Section.
Embodiment 1:
(1) cuttings selects
It is water-soluble using mixing 1600 times of mixing of sterilizing insecticide 3 days before acquiring cuttings using masson pine tissue-cultured seedling as mother plant for cutting
Liquid sprays 1 time mother plant for cutting progress comprehensively；Select 8 cm of current growth, 2.5 mm of diameter, the branch of the preferable no disease and pests harm of growing way
Item is cuttings；The mixing sterilizing insecticide is by 70% Wettable propineb powder, 60% difenoconazole wettable powder
The wettable powder being mixed to get with 80% metrifonate wettable powder according to the ratio that volume ratio is 1:1:1；The cuttings is
The masson pine branch of complete lignifying；
(2) Pretreatment
1. the cuttings that will be collected extracts the needle grown in 3 cm of base portion, being tied 25 plants with rubber band is a bundle, lower end
Keep smooth, immersing mass concentration rapidly is to carry out cuttings disinfection treatment in 0.4% chlorobromide isocyanuric acid aqueous solution, impregnates 4 points
Zhong Houyong clear water is rinsed well for use, and cuttings soaking depth is 3.5 cm；
2. the cuttings after disinfection is immersed promoter to impregnate 30 minutes, cuttings soaking depth is 1 cm；The promoter is by following
Density component composition: IAA 250 mg/L, ABT1250 mg/L, paclobutrazol 12 mg/L, VB12 15 mg/L、VE 15 mg/L、
120 mg/L of niacin, biotin 80 mg/L, KNO3 1200 mg/L、Mg(NO3)2·6H2O 250 mg/L、H3BO3 20 mg/
L、Na2S2O3273 mg/L；
(3) Water culture
1. cuttage is handled
It is by volume that 4:1 is uniformly mixed into culture solution and falls back and trains in device by promoter and water, the depth of culture solution is
4.5 cm；The fringe bar obtained by step (2) processing is inserted into hydroponic device, it is 1.5 that fringe bar base portion, which is inserted into medium depth,
cm；
It is ventilated, is ventilated 15 minutes using culture solution of the inflator pump timing into hydroponic device within every 2 hours；Until cuttings base portion callus
It organizes the formation of or starts to grow the tip of a root；
The hydroponic device includes open bubble chamber and foam cover board；The foam cover board is detachably secured to foam
The open-mouth of case；It is the aperture of 2.0cm as cutting hole that diameter is uniformly distributed on the foam cover board；The bubble chamber bottom
Portion is equipped with water inlet, water outlet and inflating port；The inflating port is connect with inflator pump；If fringe bar is not fixed easily, sponge can be used
Or cotton fills cutting hole；
2. daily management
Culture solution is primary every replacement in 5 days, to remove the impurity in culture solution in time；If should be clear in time it was found that cuttings growing way is bad
It removes；When summer weather sweltering heat strong sunlight, sunshade protection is carried out to hydroponic device with sunshade net in time, while spraying to fringe bar
Mist moisturizing, autumn, winter build plastic canopy radix saposhnikoviae.
Embodiment 2:
(1) cuttings selects
It is water-soluble using mixing 1800 times of mixing of sterilizing insecticide 3 days before acquiring cuttings using masson pine tissue-cultured seedling as mother plant for cutting
Liquid sprays 1 time mother plant for cutting progress comprehensively；Select 6 cm of current growth, 2.0 mm of diameter, the branch of the preferable no disease and pests harm of growing way
Item is cuttings；The mixing sterilizing insecticide is by 70% Wettable propineb powder, 60% difenoconazole wettable powder
The wettable powder being mixed to get with 80% metrifonate wettable powder according to the ratio that volume ratio is 1:1:1；The cuttings is
The masson pine branch of complete lignifying；
(2) Pretreatment
1. the cuttings that will be collected extracts the needle grown in 3 cm of base portion, being tied 20 plants with rubber band is a bundle, lower end
Keep smooth, immersing mass concentration rapidly is to carry out cuttings disinfection treatment in 0.5% chlorobromide isocyanuric acid aqueous solution, impregnates 4.5
It is rinsed well for use after minute with clear water, cuttings soaking depth is 3.2 cm；
2. the cuttings after disinfection is immersed promoter to impregnate 35 minutes, cuttings soaking depth is 2 cm；The promoter is by following
Density component composition: IAA 200 mg/L, ABT1300 mg/L, paclobutrazol 13 mg/L, VB12 10 mg/L、VE 10 mg/L、
130 mg/L of niacin, biotin 60 mg/L, KNO3 1200 mg/L、Mg(NO3)2·6H2O 250 mg/L、H3BO3 20 mg/
L、Na2S2O3273 mg/L；
(3) Water culture
1. cuttage is handled
It is by volume that 4:1 is uniformly mixed into culture solution and falls back and trains in device by promoter and water, the depth of culture solution is
4.5 cm；The fringe bar obtained by step (2) processing is inserted into hydroponic device, it is 2 that fringe bar base portion, which is inserted into medium depth,
cm；
It is ventilated, is ventilated 15 minutes using culture solution of the inflator pump timing into hydroponic device within every 2 hours；Until cuttings base portion callus
It organizes the formation of or starts to grow the tip of a root；
The hydroponic device includes open bubble chamber and foam cover board；The foam cover board is detachably secured to foam
The open-mouth of case；It is the aperture of 2.0 cm as cutting hole that diameter is uniformly distributed on the foam cover board；The bubble chamber
Bottom is equipped with water inlet, water outlet and inflating port；The inflating port is connect with inflator pump；If fringe bar is not fixed easily, sea can be used
Continuous or cotton fills cutting hole；
2. daily management
Culture solution is primary every replacement in 5 days, to remove the impurity in culture solution in time；If should be clear in time it was found that cuttings growing way is bad
It removes；When summer weather sweltering heat strong sunlight, sunshade protection is carried out to hydroponic device with sunshade net in time, while spraying to fringe bar
Mist moisturizing, autumn, winter build plastic canopy radix saposhnikoviae.
Embodiment 3:
(1) cuttings selects
It is water-soluble using mixing 1700 times of mixing of sterilizing insecticide 3 days before acquiring cuttings using masson pine tissue-cultured seedling as mother plant for cutting
Liquid sprays 1 time mother plant for cutting progress comprehensively；Select 5 cm of current growth, 1.5 mm of diameter, the branch of the preferable no disease and pests harm of growing way
Item is cuttings；The mixing sterilizing insecticide is by 70% Wettable propineb powder, 60% difenoconazole wettable powder
The wettable powder being mixed to get with 80% metrifonate wettable powder according to the ratio that volume ratio is 1:1:1；The cuttings is
The masson pine branch of semi-lignified；
(2) Pretreatment
1. the cuttings that will be collected extracts the needle grown in 3 cm of base portion, being tied 30 plants with rubber band is a bundle, lower end
Keep smooth, immersing mass concentration rapidly is to carry out cuttings disinfection treatment in 0.4% chlorobromide isocyanuric acid aqueous solution, impregnates 5 points
Zhong Houyong clear water is rinsed well for use, and cuttings soaking depth is 3 cm；
2. the cuttings after disinfection is immersed promoter to impregnate 35 minutes, cuttings soaking depth is 1.5 cm；The promoter by with
Lower density component composition: IAA 300 mg/L, ABT1200 mg/L, paclobutrazol 15 mg/L, VB12 10 mg/L、VE 10 mg/
L, 100 mg/L of niacin, biotin 90 mg/L, KNO3 1200 mg/L、Mg(NO3)2·6H2O 250 mg/L、H3BO3 20
mg/L、Na2S2O3273 mg/L；
(3) Water culture
1. cuttage is handled
It is by volume that 4:1 is uniformly mixed into culture solution and falls back and trains in device by promoter and water, the depth of culture solution is
4.5 cm；The fringe bar obtained by step (2) processing is inserted into hydroponic device, it is 1 that fringe bar base portion, which is inserted into medium depth,
cm；
It is ventilated, is ventilated 15 minutes using culture solution of the inflator pump timing into hydroponic device within every 2 hours；Until cuttings base portion callus
It organizes the formation of or starts to grow the tip of a root；
The hydroponic device includes open bubble chamber and foam cover board；The foam cover board is detachably secured to foam
The open-mouth of case；It is the aperture of 2.0cm as cutting hole that diameter is uniformly distributed on the foam cover board；The bubble chamber bottom
Portion is equipped with water inlet, water outlet and inflating port；The inflating port is connect with inflator pump；If fringe bar is not fixed easily, sponge can be used
Or cotton fills cutting hole；
2. daily management
Culture solution is primary every replacement in 5 days, to remove the impurity in culture solution in time；If should be clear in time it was found that cuttings growing way is bad
It removes；When summer weather sweltering heat strong sunlight, sunshade protection is carried out to hydroponic device with sunshade net in time, while spraying to fringe bar
Mist moisturizing, autumn, winter build plastic canopy radix saposhnikoviae.
Embodiment 4:
(1) cuttings selects
It is water-soluble using mixing 1500 times of mixing of sterilizing insecticide 3 days before acquiring cuttings using masson pine tissue-cultured seedling as mother plant for cutting
Liquid sprays 1 time mother plant for cutting progress comprehensively；Select 10 cm of current growth, 3.5 mm of diameter, the branch of the preferable no disease and pests harm of growing way
Item is cuttings；The mixing sterilizing insecticide is by 70% Wettable propineb powder, 60% difenoconazole wettable powder
The wettable powder being mixed to get with 80% metrifonate wettable powder according to the ratio that volume ratio is 1:1:1；The cuttings is
The masson pine branch of complete lignifying；
(2) Pretreatment
1. the cuttings that will be collected extracts the needle grown in 3 cm of base portion, being tied 20 plants with rubber band is a bundle, lower end
Keep smooth, immersing mass concentration rapidly is to carry out cuttings disinfection treatment in 0.5% chlorobromide isocyanuric acid aqueous solution, impregnates 4 points
Zhong Houyong clear water is rinsed well for use, and cuttings soaking depth is 4cm；
2. the cuttings after disinfection is immersed promoter to impregnate 45 minutes, cuttings soaking depth is 1.2 cm；The promoter by with
Lower density component composition: IAA 300 mg/L, ABT1250 mg/L, paclobutrazol 10 mg/L, VB12 20 mg/L、VE 20 mg/
L, 150 mg/L of niacin, biotin 50 mg/L, KNO3 1200 mg/L、Mg(NO3)2·6H2O 250 mg/L、H3BO3 20
mg/L、Na2S2O3273 mg/L；
(3) Water culture
1. cuttage is handled
It is by volume that 4:1 is uniformly mixed into culture solution and falls back and trains in device by promoter and water, the depth of culture solution is
4.5 cm；The fringe bar obtained by step (2) processing is inserted into hydroponic device, it is 2 that fringe bar base portion, which is inserted into medium depth,
cm；
It is ventilated, is ventilated 15 minutes using culture solution of the inflator pump timing into hydroponic device within every 2 hours；Until cuttings base portion callus
It organizes the formation of or starts to grow the tip of a root；
The hydroponic device includes open bubble chamber and foam cover board；The foam cover board is detachably secured to foam
The open-mouth of case；It is the aperture of 2.0 cm as cutting hole that diameter is uniformly distributed on the foam cover board；The bubble chamber
Bottom is equipped with water inlet, water outlet and inflating port；The inflating port is connect with inflator pump；If fringe bar is not fixed easily, sea can be used
Continuous or cotton fills cutting hole；
2. daily management
Culture solution is primary every replacement in 5 days, to remove the impurity in culture solution in time；If should be clear in time it was found that cuttings growing way is bad
It removes；When summer weather sweltering heat strong sunlight, sunshade protection is carried out to hydroponic device with sunshade net in time, while spraying to fringe bar
Mist moisturizing, autumn, winter build plastic canopy radix saposhnikoviae.
Embodiment 5:
(1) cuttings selects
It is water-soluble using mixing 1600 times of mixing of sterilizing insecticide 3 days before acquiring cuttings using masson pine tissue-cultured seedling as mother plant for cutting
Liquid sprays 1 time mother plant for cutting progress comprehensively；Select 9 cm of current growth, 3.0 mm of diameter, the branch of the preferable no disease and pests harm of growing way
Item is cuttings；The mixing sterilizing insecticide is by 70% Wettable propineb powder, 60% difenoconazole wettable powder
The wettable powder being mixed to get with 80% metrifonate wettable powder according to the ratio that volume ratio is 1:1:1；The cuttings is
The masson pine branch of semi-lignified；
(2) Pretreatment
1. the cuttings that will be collected extracts the needle grown in 3 cm of base portion, being tied 25 plants with rubber band is a bundle, lower end
Keep smooth, immersing mass concentration rapidly is to carry out cuttings disinfection treatment in 0.4% chlorobromide isocyanuric acid aqueous solution, impregnates 3.5
It is rinsed well for use after minute with clear water, cuttings soaking depth is 4 cm；
2. the cuttings after disinfection is immersed promoter to impregnate 40 minutes, cuttings soaking depth is 1.6 cm；The promoter by with
Lower density component composition: IAA 280 mg/L, ABT1200 mg/L, paclobutrazol 14 mg/L, VB12 15 mg/L、VE 15 mg/
L, 140 mg/L of niacin, biotin 100 mg/L, KNO3 1200 mg/L、Mg(NO3)2·6H2O 250 mg/L、H3BO3 20
mg/L、Na2S2O3273 mg/L；
(3) Water culture
1. cuttage is handled
It is by volume that 4:1 is uniformly mixed into culture solution and falls back and trains in device by promoter and water, the depth of culture solution is
4.5 cm；The fringe bar obtained by step (2) processing is inserted into hydroponic device, it is 2 that fringe bar base portion, which is inserted into medium depth,
cm；
It is ventilated, is ventilated 15 minutes using culture solution of the inflator pump timing into hydroponic device within every 2 hours；Until cuttings base portion callus
It organizes the formation of or starts to grow the tip of a root；
The hydroponic device includes open bubble chamber and foam cover board；The foam cover board is detachably secured to foam
The open-mouth of case；It is the aperture of 2.0 cm as cutting hole that diameter is uniformly distributed on the foam cover board；The bubble chamber
Bottom is equipped with water inlet, water outlet and inflating port；The inflating port is connect with inflator pump；If fringe bar is not fixed easily, sea can be used
Continuous or cotton fills cutting hole；
2. daily management
Culture solution is primary every replacement in 5 days, to remove the impurity in culture solution in time；If should be clear in time it was found that cuttings growing way is bad
It removes；When summer weather sweltering heat strong sunlight, sunshade protection is carried out to hydroponic device with sunshade net in time, while spraying to fringe bar
Mist moisturizing, autumn, winter build plastic canopy radix saposhnikoviae.
Embodiment 6:
(1) cuttings selects
It is water-soluble using mixing 1650 times of mixing of sterilizing insecticide 3 days before acquiring cuttings using masson pine tissue-cultured seedling as mother plant for cutting
Liquid sprays 1 time mother plant for cutting progress comprehensively；Select 6 cm of current growth, 1.5 mm of diameter, the branch of the preferable no disease and pests harm of growing way
Item is cuttings；The mixing sterilizing insecticide is by 70% Wettable propineb powder, 60% difenoconazole wettable powder
The wettable powder being mixed to get with 80% metrifonate wettable powder according to the ratio that volume ratio is 1:1:1；The cuttings is
The masson pine branch of complete lignifying；
(2) Pretreatment
1. the cuttings that will be collected extracts the needle grown in 3 cm of base portion, being tied 25 plants with rubber band is a bundle, lower end
Keep smooth, immersing mass concentration rapidly is to carry out cuttings disinfection treatment in 0.3% chlorobromide isocyanuric acid aqueous solution, impregnates 3 points
Zhong Houyong clear water is rinsed well for use, and cuttings soaking depth is 3.2 cm；
2. the cuttings after disinfection is immersed promoter to impregnate 45 minutes, cuttings soaking depth is 1.8cm；The promoter by with
Lower density component composition: IAA 250 mg/L, ABT1200 mg/L, paclobutrazol 10 mg/L, VB12 20 mg/L、VE 20 mg/
L, 150 mg/L of niacin, biotin 90 mg/L, KNO3 1200 mg/L、Mg(NO3)2·6H2O 250 mg/L、H3BO3 20
mg/L、Na2S2O3273 mg/L；
(3) Water culture
1. cuttage is handled
It is by volume that 4:1 is uniformly mixed into culture solution and falls back and trains in device by promoter and water, the depth of culture solution is
4.5 cm；The fringe bar obtained by step (2) processing is inserted into hydroponic device, it is 1.5 that fringe bar base portion, which is inserted into medium depth,
cm；
It is ventilated, is ventilated 15 minutes using culture solution of the inflator pump timing into hydroponic device within every 2 hours；Until cuttings base portion callus
It organizes the formation of or starts to grow the tip of a root；
The hydroponic device includes open bubble chamber and foam cover board；The foam cover board is detachably secured to foam
The open-mouth of case；It is the aperture of 2.0 cm as cutting hole that diameter is uniformly distributed on the foam cover board；The bubble chamber
Bottom is equipped with water inlet, water outlet and inflating port；The inflating port is connect with inflator pump；If fringe bar is not fixed easily, sea can be used
Continuous or cotton fills cutting hole；
2. daily management
Culture solution is primary every replacement in 5 days, to remove the impurity in culture solution in time；If should be clear in time it was found that cuttings growing way is bad
It removes；When summer weather sweltering heat strong sunlight, sunshade protection is carried out to hydroponic device with sunshade net in time, while spraying to fringe bar
Mist moisturizing, autumn, winter build plastic canopy radix saposhnikoviae.
- 6 method in accordance with the above-mentioned embodiment 1, observation statistics rooting rate, rooting rate=(list of taking root after Water culture 30 days
Strain number/this group of cuttings sum) × 100%, concrete outcome such as table 1.
Table 1 carries out the obtained rooting rate of masson pine Water culture nursery according to the method for embodiment 1-6
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment only includes one
A independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should will say
As a whole, the technical solutions in the various embodiments may also be suitably combined for bright book, and forming those skilled in the art can be with
The other embodiments of understanding.
Claims (4)
1. a kind of masson pine Water culture method for culturing seedlings, it is characterised in that: including cuttings selects, Pretreatment and water planting skewer
Insert, using masson pine tissue-cultured seedling as mother plant for cutting, acquisition current-year branch be cuttings, to cuttings progress Pretreatment after in water planting
Water culture and daily management are carried out in case, the final masson pine cuttage seeding for obtaining raised growth stalwartness, main operational steps are such as
Under:
(1) cuttings selects
It is mixed using 1500~1800 times of sterilizing insecticide of mixing 3 days before acquiring cuttings using masson pine tissue-cultured seedling as mother plant for cutting
Heshui solution sprays 1 time mother plant for cutting progress comprehensively；Select 5~10 cm of current growth, 1.5~3.5 mm of diameter, growing way compared with
The branch of good no disease and pests harm is cuttings；
(2) Pretreatment
1. the cuttings that will be collected extracts the needle grown in 3 cm of base portion, being tied 20~30 plants with rubber band is a bundle,
Lower end keeps smooth, and immersing mass concentration rapidly is to carry out at cuttings disinfection in 0.3~0.5% chlorobromide isocyanuric acid aqueous solution
Reason, immersion 3~rinsed well for use with clear water after five minutes, cuttings soaking depth are 3~4 cm；
2. the cuttings after disinfection is immersed promoter to impregnate 30~45 minutes, cuttings soaking depth is 1~2 cm；The promoting root growth
Agent is made of following density component: IAA 200-300 mg/L, ABT1200-300 mg/L, paclobutrazol 10-15 mg/L, VB12
10-20 mg/L, VE 10-20 mg/L, niacin 100-150 mg/L, biotin 50-100 mg/L, KNO3 1200 mg/L、
Mg(NO3)2·6H2O 250 mg/L、H3BO3 20 mg/L、Na2S2O3 273 mg/L；
(3) Water culture
1. cuttage is handled
It is by volume that 4:1 is uniformly mixed into culture solution and falls back and trains in device by promoter and water, the depth of culture solution is
4.5 cm；The fringe bar obtained by step (2) processing is inserted into hydroponic device, it is 1~2 that fringe bar base portion, which is inserted into medium depth,
cm；
It is ventilated, is ventilated 15 minutes within every 2 hours, until cuttings base portion callus using culture solution of the inflator pump timing into hydroponic device
It organizes the formation of or starts to grow the tip of a root；
2. daily management
Culture solution is primary every replacement in 5 days, to remove the impurity in culture solution in time；If should be clear in time it was found that cuttings growing way is bad
It removes；When summer weather sweltering heat strong sunlight, sunshade protection is carried out to hydroponic device with sunshade net in time, while spraying to fringe bar
Mist moisturizing, autumn, winter build plastic canopy radix saposhnikoviae.
2. a kind of masson pine Water culture method for culturing seedlings according to claim 1, it is characterised in that: in the step (1)
Mixing sterilizing insecticide be can by 70% Wettable propineb powder, 60% difenoconazole wettable powder and 80% metrifonate
The wettable powder that wet powder is mixed to get according to the ratio that volume ratio is 1:1:1.
3. a kind of masson pine Water culture method for culturing seedlings according to claim 1, it is characterised in that: in the step (1)
Cuttings be semi-lignified or complete lignifying masson pine branch.
4. a kind of masson pine Water culture method for culturing seedlings according to claim 1, it is characterised in that: in the step (3)
Hydroponic device include open bubble chamber and foam cover board；The foam cover board is detachably secured to the opening of bubble chamber
Place；It is the aperture of 2.0cm as cutting hole that diameter is uniformly distributed on the foam cover board；The bubble chamber bottom be equipped with into
The mouth of a river, water outlet and inflating port；The inflating port is connect with inflator pump；If fringe bar is not fixed easily, can be filled out with sponge or cotton
Fill cutting hole.
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| CN110896854A (en) * | 2019-08-22 | 2020-03-24 | 北京林业大学 | Culture method for promoting development and maturation of somatic embryos of Chinese pine |
| CN114230418A (en) * | 2022-01-07 | 2022-03-25 | 贵州大学 | Water culture nutrient solution and water culture method for pinus massoniana seedlings |
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| CN110896854A (en) * | 2019-08-22 | 2020-03-24 | 北京林业大学 | Culture method for promoting development and maturation of somatic embryos of Chinese pine |
| CN110896854B (en) * | 2019-08-22 | 2021-05-11 | 北京林业大学 | Culture method for promoting development and maturation of somatic embryos of Chinese pine |
| CN114230418A (en) * | 2022-01-07 | 2022-03-25 | 贵州大学 | Water culture nutrient solution and water culture method for pinus massoniana seedlings |
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