CN108243817B - A kind of Mongolian oak green branch cuttage breeding method - Google Patents
A kind of Mongolian oak green branch cuttage breeding method Download PDFInfo
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- A—HUMAN NECESSITIES
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Abstract
A kind of Mongolian oak green branch cuttage breeding method, comprising the following steps: seedling stage pretreatment;Slotting machine processing;Adopt the fringe time;The shearing of cuttings;Cuttage medicament;Cutting density and depth;Slotting machine temperature and humidity;It is managed after cuttage;Transplanting.A kind of Mongolian oak green branch cuttage breeding method provided by the invention, easy to operate, nursery speed is fast, and breeding coefficient is high, and dosage is few, at low cost, more environmentally friendly, has good market application prospect.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, and in particular to a kind of Mongolian oak green branch cuttage breeding method.
Background technique
Mongolian oak (QuercusmongolicaFisch. ex Ledeb.), Fagaceae (Fagaceae), oak category
(QuercusIt Linn.), is deciduous tree.Deep-rootedness, it is drought-resistant, barren-resistant, be tree species most cold-resistant in oak plant (- 50 ~
- 60 DEG C remain to survive).Soil pH value 6.0 ~ 6.5 is born in happiness.Its blade is as large-scale Silk-producing insects tussah
(AntheraeapernyiGu é rin-M é neville) one of high-quality feed, in Liaoning, Jilin, Heilungkiang, Inner Mongol etc.
Provinces and regions are widely applied.The earliest robur of leaf is opened as spring, is particularly suitable for putting in a suitable place to breed for spring young silkworm.
Mongolian oak material is hard, and corrosion resistance is strong, and for the material such as car and boat, building, mine timber, compressed wood can make machinery zero
Part.Seed can make wine or make feed, and bark is used as medicine the benefits of having astringing to arrest diarrhea and controlling dysentery.Duan Mu and sawdust can produce edible fungus
With firewood charcoal.Mongolian oak does not form the constructive species of the Northeast's Characteristlcs of The Forest Flora and the group of North China forest plants still
At part, have check winds and fix drifting sand, conserve water and soil, the important Ecology Action such as water conservation.
Currently, the main Mongolian oak standing forest overwhelming majority in China is the scondary forest that sprout tillers updates after felling, heredity changes
Good degree is poor, standing forest output is low.Mongolian oak artificial propagation mode is almost all the sexual propagation of seed progress.And Mongolian oak
The treelet phase is 15 ~ 20 years, and with the numerous seedling of seed, the period is longer, not can guarantee excellent characteristic and stablizes heredity.In addition Mongolian oak acorn worm
Mouth rate is very heavy, and in the usual time, acorn insect population rate is up to 90% or more, and with its nursery, breeding coefficient is lower, higher cost, can not carry out
A large amount of numerous seedlings.Vegetative propagation includes grafting, tissue cultures, cuttage etc., can keep parent's whole merit.For this purpose, some special
Family scholar has carried out correlative study.Grafting aspect, due to Mongolian oak grafting disaffinity etc., there is loose tumor scar at joint portion,
Healing effect is poor, and survival rate is unstable, and breeding coefficient is low, can not accomplish the fast numerous of excellent nursery stock.The tissue cultures of Mongolian oak are most
Pipe existing research, but fail practical application.Cuttage has reproduction speed fast, and easy to operate, cost is relatively low, can be effectively ensured original
Merit stablizes the advantage of heredity.Mongolian oak is cuttage tree species difficult to take root, although from 1970s, the cuttage of Mongolian oak
Research has just started, but rooting rate is lower always.
The expert of Inst. of Sericulture Science, Liaoning Prov. has carried out raw Mongolian oak current year green wood cutting test in 1 year in 1977,
Rooting rate is up to 38.5%(Wang Chang outstanding person etc., and 1983).It takes root mostly 1, and transplanting has no and survives.Heilungkiang band ridge forestry section
It learns research institute and had once made the test of Mongolian oak green wood cutting, totally 9 processing handle time 2h with fringe bar 90, do not provide specific
Reagent combination is had no way of with reference to (Jin Jihua, 2004).China Forestry Science Research Institute has made Mongolian oak current year green wood cutting examination
It tests, rooting rate is up to 16.67% (Huang Qin army etc., 2013).With current year raw Mongolian oak middle and upper part shoot cutting, rooting rate highest
For 3.3%(Yan Wen great waves etc., 2017).The studies above result: Mongolian oak cutting propagation coefficient is relatively low.How Mongolian oak cuttage is improved
Breeding coefficient is one of the difficult point of current Mongolian oak vegetative propagation.
Summary of the invention
The object of the present invention is to provide a kind of Mongolian oak green branch cuttage breeding methods, and this method is easy to operate, production cost
Low, growing-seedling period is short, and dosage is few, more environmentally friendly, requires cuttings length not tight, rooting rate and the high advantage of transplanting survival rate.
The technical scheme is that
A kind of Mongolian oak green branch cuttage breeding method, comprising the following steps:
1. seedling stage pre-processes: the seedling stage pretreatment is that 2 years raw select trees are chosen in the cutting orchard that fine provenance is built up
Seedling before Yu Chunji sap is sprouted, cuts off top branch from rootstock.3 ~ 5 days before cuttage, cutting orchard select region, in
Ridge platform side hammers into timber, and 1 ~ 2m of pilespacing ties up sunshade net on timber, the nursery stock sunshade that will be selected in cutting orchard with sunshade net
60%~85%。
2. slotting machine is handled: slotting machine processing is slotting machine long 1 ~ 12m, wide 0.6 ~ 1.5m in making slotting machine in greenhouse, deep 7
~ 20cm, high 45 ~ 65cm build arched frame, the high 55 ~ 60cm of frame on slotting machine.Cutting medium is laid in slotting machine, matrix is treasure
Zhu Yan, particle size are 2 ~ 5mm, and laying depth is 7 ~ 20cm, 1 ~ 3 day before cuttage, with 80% carbendazol wettable powder
500 ~ 1000 times of liquid or 75% 500 ~ 1000 times of liquid of Bravo wettable powder in a kind of progress matrix disinfection.
3. adopting the fringe time: described to adopt the fringe time as 5 ~ September, clip is grown from the cutting orchard that fine germplasm resources are built up
It is healthy and strong, blade is complete, no disease and pests harm semi-lignified branch.
4. the shearing of cuttings: the shearing of the cuttings is that top fringe bar is cut into the cuttings of 2 ~ 20cm, only stays top
The 1/2 of 2 ~ 3 leaves, rest blade is all cut, the angle beveling of 45 degree of lower part.50 lower end bundles of every bundle are neat, are immersed in 80%
In 600 ~ 800 times of liquid of carbendazol wettable powder, 20 ~ 30min of disinfection is taken out, and impregnates cuttage medicine again after branch lower end is slightly dry
Agent.
5. cuttage medicament: the cuttage medicament is 25 ~ 500mg of 3- indolebutyric acid L-1(Shenyang City East China chemical reagent work) and/
Or 25 ~ 500mg of methyl α-naphthyl acetate L-1(Shenyang City East China chemical reagent work), No. ABT1 Beijing 50 ~ 500mg L-1(Ai Bidi biotechnology have
Limit company), 6- benzyl 1 ~ 15mg of aminoadenine L-1(Sinopharm Chemical Reagent Co., Ltd.), 100 ~ 1000mg of vitamin L-1, 5 ~ 20mg of diethyl aminoethyl hexanoate L-1Mixed liquor.0.5 ~ 6h of chemicals treatment time.
6. cutting density, depth: the cutting density is 150 ~ 270/m2, cuttage depth is 1 ~ 10cm.It is beaten before cuttage
Plastic film is opened, draws hole cuttage with thickness disinfection waddy equal with cuttings or slightly thick, depth is consistent with cuttings.It is light after having inserted
Pressure is poured all over permeable.The plastic covering film on arched frame, it is sealed around, then at covering sunshade net thereon, sunshade 4 ~ 7 days, shading
Rate 60% ~ 80%.
7. cuttage temperature and humidity: 16 ~ 26 DEG C of the slotting machine substrate temperature, substrate water content are 80% ~ 93%, opposite in arched shed
Humidity is maintained at 85% ~ 100%.
8. managing after cuttage: management is that sunshade is 4 ~ 5 days after cuttage after the cuttage, shading rate 60% ~ 70%.Every the sky,
Afternoon each atomized water spray 1 time;Pour weekly 1 time it is permeable, and alternately spray 80% 500 ~ 1000 times of liquid of carbendazol wettable powder and 75%
500 ~ 1000 times of Bravo wettable powder liquid 1 time.
9. transplanting: stone, the soil block in fertile soil being screened out with sieve in advance, fertile soil, turfy soil, perlite are pressed
Weight ratio 5:1:1 is uniformly mixed.On crop field boring ridge, 55 ~ 75cm of row spacing ditches on the platform of ridge, 7 ~ 12cm of ditch depth.By aforementioned mixing
Object is spread in furrow, is poured all over permeable.Transplanting time is cuttage 35 ~ 40 days, plants seedling by 10 ~ 25cm of spacing in the rows, natural according to cuttings root
The direction of growth is planted into furrow, and gently earthing, whole cover sunshade net 7 ~ 18 days after planting well since root lower part, Yu Yintian or fine
Sunshade net is opened in front and back at dusk for it.
Advantages of the present invention:
The present invention provides a kind of technology and methods that can refer to for the numerous seedling of Mongolian oak green wood cutting, with prior art phase
Than having the advantage that
1. propagation method is simple, transplanting survival rate is high.Method is simple, and general worker can be operated.Raw Mongolian oak is worked as within 2 years
Year green wood cutting, takes root, 35 ~ 40 days rooting rates are up to 86.2%, and longest root 11.5cm, root is up to 9,2 grades of sides for 7 ~ 10 days
Root is more.Field-transplanting survival rate is up to 95.6%.
2. management is simple, save the cost.Cutting medium only uses perlite, makes the bed and sterilisation step is simple, laborsaving, especially
It is that Final pollution is few, management is simple.The cuttings leaching medicine time is short, and rooting rate is fast, and working hour expense is few, saves labor cost.Transplanting
Perlite used is that cuttage is used (waste utilization, miscellaneous bacteria are few), meanwhile, the addition of diethyl aminoethyl hexanoate decreases plant growth tune
The usage amount for saving agent, fungicide, reduces production cost.
3. medication is few, more environmentally friendly.Diethyl aminoethyl hexanoate is added, synergistic effect can be played, reduces plant growth regulator, sterilization
The usage amount of agent, fertilizer etc..Diethyl aminoethyl hexanoate is a kind of efficient plant growth regulator, nontoxic, without side-effects, noresidue.It can make to increase
It is mixed with other plant growth regulator, fungicide, fertilizer etc. to imitate agent, synergy 10% ~ 20%, with the compatibility of ecological environment compared with
It is good.With the incomparable advantage of other plant growth regulator.
4. cutting length require it is not tight, it is more for the fringe bar material that utilizes.
Cuttage medicament of the present invention and method are not only limited in Mongolian oak, to cork oak, Mongolian oak, Quercus liaotungensis, Mongolian oak
The oak plants such as oak, acuteserrata, Serrata oak, daimyo oak, toothed oak oriental white oak, Fengcheng City oak also have rooting effect.
Specific embodiment
Embodiment 1
A kind of Mongolian oak green branch cuttage breeding method, comprising the following steps:
1. seedling stage pre-processes: selecting 2 years raw select tree seedlings in the cutting orchard that fine provenance is built up, Yu Chunji sap is sprouted
Before, top branch is cut off from rootstock.4 days before cuttage, in the region that cutting orchard is selected, timber, stake are hammered into ridge platform side
Away from 1.5m, sunshade net is tied up on timber, sunshade 70%.
2. slotting machine is handled: slotting machine is set in Inst. of Sericulture Science, Liaoning Prov. greenhouse, each long 8m of slotting machine, wide 0.9m, deep
13cm, high 55cm.Cutting medium is laid in slotting machine, matrix is perlite, 2 ~ 5mm of particle size.Laying depth is 12cm, paving
In taking arched frame, the high 60cm of frame on bed after complete.Before cutting fringe 1 day give matrix disinfection, first matrix is sprinkled profoundly water, then with 80% it is more
1000 times of liquid of bacterium spirit wettable powder carry out matrix disinfection, the plastic covering film on arched frame.
3. adopting the fringe time: described to adopt the fringe time as June, clip growth is strong from the cutting orchard that fine germplasm resources are built up
It strengthens, blade is complete, no disease and pests harm semi-lignified branch.
4. the shearing of cuttings: rinsing the dust on branch surface with clear water, fringe bar is cut into inserting for long 9 ~ 10cm from top
Fringe only stays the 1/2 of 2 ~ 3 leaves of top, and rest blade is all cut off, the angle beveling of 45 degree of lower part.50 lower end cotton ropes of every bundle
Bundle is neat, is immersed in 80% 600 ~ 800 times of liquid of carbendazol wettable powder, and disinfection 25min takes out, and omits to branch lower end
Cuttage medicament is impregnated again after dry.
5. cuttage medicament: the cuttage medicament is 3- indolebutyric acid 150mg L-1With methyl α-naphthyl acetate 50mg L-1, No. ABT1
100mg•L-1, 6- benzyl aminoadenine 3mg L-1, diethyl aminoethyl hexanoate 10mg L-1, vitamin C 400mg L-1Mixed liquor.At medicament
Manage time 0.5h.
6. cutting density, depth: the cutting density is 200/m2, cuttage depth is 5cm.Cuttage front opening plastics
Film draws hole cuttage with thickness disinfection waddy equal with cuttings or slightly thick, and depth is consistent with cuttings.Light pressure after having inserted, is poured time
It is permeable.The plastic covering film on arched frame, it is sealed around, then at covering sunshade net thereon, sunshade 4 days, shading rate 70%.
7. cuttage temperature and humidity: 23 ~ 24 DEG C of slotting machine substrate temperature, substrate water content is 86% ~ 87%, relative humidity in arched shed
It is 94% ~ 95%.
8. managing after cuttage: management is that sunshade is 4 days after cuttage after the cuttage, shading rate 60%.Every morning, afternoon
Each atomized water spray 1 time when temperature is higher;It sprinkles profoundly water weekly 1 time, and alternately sprays 800 ~ 1000 times of carbendazol wettable powder of 80%
Liquid and 600 ~ 800 times of 75% Bravo wettable powder liquid 1 time.
9. transplanting: stone, the soil block in fertile soil being screened out with sieve in advance, fertile soil, turfy soil, perlite are pressed
Weight ratio 5:1:1 is uniformly mixed.On crop field boring ridge, row spacing 60cm ditches on the platform of ridge, 10 cm of ditch depth.Aforementioned mixture is spread
In in furrow, pouring all over permeable.Transplanting time is cuttage 35 days, plants seedling by seeding row spacing 20cm × 60cm, naturally raw according to cuttings root
Long direction is planted into furrow, the gently earthing since root lower part, all plant it is good after cover sunshade net 10 days, in fine day at dusk before and after
Open sunshade net.
Embodiment 2
A kind of Mongolian oak green branch cuttage breeding method, concrete operation method is substantially same as Example 1, and difference exists
In:
Medicament B take root by 3- indolebutyric acid 100mg L-1With methyl α-naphthyl acetate 50mg L-1, ABT1 50mg L-1, 6- benzyl amino
Adenine 3mg L-1, diethyl aminoethyl hexanoate 10mg L-1, vitamin C 400mg L-1Solution constitute.
Embodiment 3
A kind of Mongolian oak green branch cuttage breeding method, concrete operation method is substantially same as Example 1, and difference exists
In:
Medicament C take root by 3- indolebutyric acid 200mg L-1, ABT1 100mg L-1, 6- benzyl aminoadenine 3mg L-1, amine
Fresh ester 10mg L-1, vitamin C 400mg L-1Solution constitute.
Embodiment 4
A kind of Mongolian oak green branch cuttage breeding method, concrete operation method is substantially same as Example 1, and difference exists
In:
Medicament D take root by 3- indolebutyric acid 100mg L-1, ABT1 50mg L-1, 6- benzyl aminoadenine 3mg L-1, amine
Fresh ester 10mg L-1, vitamin C 400mg L-1Solution constitute.
The present inventor compares test to Mongolian oak green branch cuttage breeding method, and pair of chemicals treatment is not had to by comparing
According to area, show that different agents formula has different influences to Mongolian oak green wood cutting breeding rooting rate, specifically:
600 Mongolian oak green branch cuttings are handled using cuttage medicament A, strain number of taking root is 517 plants, and rooting rate is
86.2%, transplant survival strain number is 494 plants, transplanting survival rate 95.6%;600 Mongolian oak green branch are inserted using cuttage medicament B
Fringe is handled, and strain number of taking root is 426 plants, and rooting rate 71.0%, transplant survival strain number is 385 plants, and transplanting survival rate is
90.4%;600 Mongolian oak green branch cuttings are handled using cuttage medicament C, strain number of taking root is 481 plants, and rooting rate is
80.2%, transplant survival strain number is 447 plants, transplanting survival rate 93.0%;600 Mongolian oak green branch are inserted using cuttage medicament D
Fringe is handled, and strain number of taking root is 407 plants, and rooting rate 67.8%, transplant survival strain number is 359 plants, and transplanting survival rate is
88.2%。
300 Mongolian oak green branch cuttings are handled using distilled water, strain number of taking root is 0 plant.
Test proves: being handled using cuttage medicament A Mongolian oak green branch, using effect is higher than other root-growing agents, raw
Root rate is 86.2%, transplanting survival rate also highest, is 95.6%.Specific concentration and formula B ~ D above-mentioned using the present invention, effect of taking root
Fruit A of the present invention compared with medicament is low.
Claims (4)
1. a kind of Mongolian oak green branch cuttage breeding method, it is characterised in that: the following steps are included:
(1) seedling stage pre-processes: the seedling stage pretreatment is that 2 years excellent saplings of life are chosen in the cutting orchard that fine provenance is built up,
Before sap is sprouted in spring, top branch is cut off from rootstock;3 ~ 5 days before cuttage, in the region that cutting orchard is selected, in ridge platform
Side hammers into timber, and 1 ~ 2m of pilespacing ties up sunshade net on timber, with sunshade net by the nursery stock sunshade 60% selected in cutting orchard ~
85%;
(2) slotting machine is handled: slotting machine processing is slotting machine long 1 ~ 12m, wide 0.6 ~ 1.5m in making slotting machine in greenhouse, deep 7 ~
20cm, high 45 ~ 65cm build arched frame, the high 55 ~ 60cm of frame on slotting machine;Cutting medium is laid in slotting machine, matrix is pearl
Rock, particle size be 2 ~ 5mm, laying depth be 7 ~ 20cm, 1 ~ 3 day before cuttage, with 80% carbendazol wettable powder 500 ~
1000 times of liquid or 75% 500 ~ 1000 times of liquid of Bravo wettable powder in a kind of progress matrix disinfection;
(3) adopt the fringe time: it is described adopt the fringe time be 5 ~ September, from the cutting orchard that fine germplasm resources are built up clip robust growth,
Blade is complete, no disease and pests harm semi-lignified branch;
(4) shearing of cuttings: the shearing of the cuttings is that top fringe bar is cut into the cuttings of 2 ~ 20cm, only stays top 2 ~ 3
The 1/2 of piece leaf, rest blade is all cut, the angle beveling of 45 degree of lower part;50 lower ends of every bundle bundle is neat, be immersed in 80% it is more
In 600 ~ 800 times of liquid of bacterium spirit wettable powder, 20 ~ 30min of disinfection is taken out, and impregnates cuttage medicament again after branch lower end is slightly dry;
(5) cuttage medicament: the cuttage medicament is 25 ~ 500mg of 3- indolebutyric acid L-1With 25 ~ 500mg of methyl α-naphthyl acetate L-1, No. ABT1
50~250mg•L-1, 6- benzyl 1 ~ 15mg of aminoadenine L-1, 5 ~ 20mg of diethyl aminoethyl hexanoate L-1, 100 ~ 1000mg of vitamin C L-1It is mixed
Close liquid;0.5 ~ 6h of chemicals treatment time;
(6) cutting density, depth: the cutting density is 150 ~ 270/m2, and cuttage depth is 5 ~ 6cm;The modeling of cuttage front opening
Expect film, draws hole cuttage with thickness disinfection waddy equal with cuttings or slightly thick, depth is consistent with cuttings;Light pressure, is poured after having inserted
All over permeable;The plastic covering film on arched frame, it is sealed around, then at covering sunshade net thereon, sunshade 4 ~ 7 days, shading rate 60%
~70%;
(7) cuttage temperature and humidity: 16 ~ 26 DEG C of the slotting machine substrate temperature, substrate water content are 80% ~ 93%, relative humidity in arched shed
It is 85% ~ 100%;
(8) manage after cuttage: management be that sunshade is 4 ~ 5 days after cuttage after the cuttage, shading rate 60% ~ 70%, daily the upper and lower noon
Each atomized water spray 1 time;Pour weekly 1 time it is permeable, and alternately spray 80% 800 ~ 1000 times of liquid of carbendazol wettable powder and 75,%00 bacterium
Clear 600 ~ 800 times of wettable powder liquid 1 time;
(9) it transplants: stone, the soil block in fertile soil being screened out with sieve in advance, by weight by fertile soil, turfy soil, perlite
It is uniformly mixed than 5:1:1;On crop field boring ridge, 55 ~ 75cm of row spacing ditches on the platform of ridge, 7 ~ 12 cm of ditch depth;By aforementioned mixture
It is spread in furrow, pours all over permeable;Transplanting time is cuttage 35 ~ 40 days, plants seedling by 10 ~ 25cm of spacing in the rows, naturally raw according to cuttings root
Long direction is planted into furrow, and gently earthing, whole cover sunshade net 7 ~ 18 days after planting well since root lower part, Yu Yintian or fine day
Sunshade net is opened in front and back at dusk.
2. a kind of Mongolian oak green branch cuttage breeding method according to claim 1, it is characterised in that:
It is described to adopt the fringe time as 6 ~ July;The shearing of the cuttings is that top fringe bar is sheared to the cuttings of 8 ~ 10cm of growth;The skewer
Medicinal stripe insertion agent is 100 ~ 250mg of 3- indolebutyric acid L-1With 50 ~ 150mg of methyl α-naphthyl acetate L-1, No. ABT1 100 ~ 200mg L-1, 6- benzyl ammonia
Base 3 ~ 10mg of adenine L-1, 300 ~ 500mg of vitamin C L-1, 10 ~ 15mg of diethyl aminoethyl hexanoate L-1Solution, the chemicals treatment time is
0.5~1h;The cutting density is 200 ~ 230/m2, cuttage depth is 5 ~ 6cm;Cuttage temperature and humidity: the slotting machine substrate temperature
23 ~ 25 DEG C, substrate water content is 86% ~ 88%, and relative humidity is 93% ~ 95% in arched shed.
3. a kind of Mongolian oak green branch cuttage breeding method, it is characterised in that: the following steps are included:
(1) seedling stage pre-processes: the seedling stage pretreatment is that 2 years excellent saplings of life are chosen in the cutting orchard that fine provenance is built up,
Before sap is sprouted in spring, top branch is cut off from rootstock;3 ~ 5 days before cuttage, in the region that cutting orchard is selected, in ridge platform
Side hammers into timber, and 1 ~ 2m of pilespacing ties up sunshade net on timber, with sunshade net by the nursery stock sunshade 60% selected in cutting orchard ~
70%;
(2) slotting machine is handled: slotting machine processing is slotting machine long 1 ~ 12m, wide 0.6 ~ 1.5m in making slotting machine in greenhouse, deep 5 ~
20cm, high 45 ~ 65cm build arched frame, the high 55 ~ 60cm of frame on slotting machine;Cutting medium is laid in slotting machine, matrix is pearl
Rock, particle size be 2 ~ 5mm, laying depth be 5 ~ 20cm, 1 ~ 3 day before cuttage, with 80% carbendazol wettable powder 500 ~
1000 times of liquid or 75% 500 ~ 1000 times of liquid of Bravo wettable powder in a kind of progress matrix disinfection;
(3) adopt the fringe time: it is described adopt the fringe time be 5 ~ September, from the cutting orchard that fine germplasm resources are built up clip robust growth,
Blade is complete, no disease and pests harm semi-lignified branch;
(4) shearing of cuttings: the shearing of the cuttings is that top fringe bar is cut into the cuttings of 3 ~ 16cm, only stays top 2 ~ 3
The 1/2 of piece leaf, rest blade is all cut, the angle beveling of 45 degree of lower part;50 lower ends of every bundle bundle is neat, be immersed in 80% it is more
In 600 ~ 800 times of liquid of bacterium spirit wettable powder, 20 ~ 30min of disinfection is taken out, and impregnates cuttage medicament again after branch lower end is slightly dry;
(5) cuttage medicament: the cuttage medicament is 100 ~ 300mg of 3- indolebutyric acid L-1, No. ABT1 50 ~ 250mg L-1, 6- benzyl ammonia
Base 1 ~ 15mg of adenine L-1, 5 ~ 20mg of diethyl aminoethyl hexanoate L-1, 200 ~ 600mg of vitamin C L-1Mixed liquor;The chemicals treatment time
0.5~6h;
(6) cutting density, depth: the cutting density is 200 ~ 230/m2, cuttage depth is 5 ~ 6cm;Cuttage front opening plastics
Film draws hole cuttage with thickness disinfection waddy equal with cuttings or slightly thick, and depth is consistent with cuttings;Light pressure after having inserted, is poured time
It is permeable;The plastic covering film on arched frame, it is sealed around, then at covering sunshade net thereon, sunshade 4 ~ 5 days, shading rate 60% ~
80%;
(7) cuttage temperature and humidity: 16 ~ 26 DEG C of the slotting machine substrate temperature, substrate water content are 80% ~ 93%, relative humidity in arched shed
It is 85% ~ 100%;
(8) manage after cuttage: management is that sunshade is 4 ~ 5 days after cuttage after the cuttage, shading rate 60% ~ 80%;The daily upper and lower noon
Each atomized water spray 1 time;Pour weekly 1 time it is permeable, and alternately spray 80% 500 ~ 1000 times of liquid of carbendazol wettable powder and 75,%00 bacterium
Clear 500 ~ 1000 times of wettable powder liquid 1 time;
(9) it transplants: stone, the soil block in fertile soil being screened out with sieve in advance, by weight by fertile soil, turfy soil, perlite
It is uniformly mixed than 5:1:1;On crop field boring ridge, 55 ~ 75cm of row spacing ditches on the platform of ridge, 7 ~ 12 cm of ditch depth;By aforementioned mixture
It is spread in furrow, pours all over permeable;Transplanting time is cuttage 35 ~ 40 days, plants seedling by 10 ~ 25cm of spacing in the rows, naturally raw according to cuttings root
Long direction is planted into furrow, and gently earthing, whole cover sunshade net 7 ~ 18 days after planting well since root lower part, Yu Yintian or fine day
Sunshade net is opened in front and back at dusk.
4. a kind of Mongolian oak green branch cuttage breeding method according to claim 3, it is characterised in that:
It is described to adopt the fringe time as 6 ~ July;The shearing of the cuttings is that top fringe bar is sheared to the cuttings of 8 ~ 10cm of growth;The skewer
Medicinal stripe insertion agent is 100 ~ 250mg of 3- indolebutyric acid L-1, No. ABT1 100 ~ 200mg L-1, 6- benzyl 3 ~ 10mg of aminoadenine L-1, dimension
Raw element 300 ~ 500mg of C L-1, 10 ~ 15mg of diethyl aminoethyl hexanoate L-1Solution, the chemicals treatment time be 0.5 ~ 1h;The cutting density
For 200 ~ 230/m2, cuttage depth is 5 ~ 6cm;Cuttage temperature and humidity: 23 ~ 25 DEG C of the slotting machine substrate temperature, substrate water content
It is 86% ~ 88%, relative humidity is 93% ~ 95% in arched shed.
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CN102577952A (en) * | 2012-02-10 | 2012-07-18 | 中国林业科学研究院亚热带林业研究所 | Tissue culturing method for quercus virginiana |
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