CN103975739B - The propagation method of a kind of Pasania cuspidata young anvil grafting container seedling - Google Patents
The propagation method of a kind of Pasania cuspidata young anvil grafting container seedling Download PDFInfo
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- CN103975739B CN103975739B CN201410207666.4A CN201410207666A CN103975739B CN 103975739 B CN103975739 B CN 103975739B CN 201410207666 A CN201410207666 A CN 201410207666A CN 103975739 B CN103975739 B CN 103975739B
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Abstract
The invention discloses the propagation method of a kind of Pasania cuspidata young anvil grafting container seedling, comprise young anvil to cultivate and propagation by grafiting operating procedure, method selects common Pasania cuspidata seed, Seed soaking 1 day is used before sowing, again with the seed dressing of chlorophos solution, then with wet husky stored seeds, the seed of germination is taken out from wet sand sowing and cultivate stock in container for plant growth, nursery stock strengthens water and fertilizer management and the extermination of disease and insect pest after being unearthed, seedling leading thread reaches more than 0.3cm and can be used for grafting, excellent Pasania cuspidata elite stand gathers the fringe bar of semi-lignified branch as grafting, adopt the grafting of cut-grafting method, plastic film is opened after young sprout is extracted in scion out, the rudiment that timely removing stock grows, but stop fertilising after entering the winter, make the abundant lignification of grafting, garden afforestation can be gone out to spring next year grafting.The present invention can keep elite stand good characteristic, and survival rate is high, and breeding method is easy, easily promotes, and is applicable to downgrading the large-scale production that Pasania cuspidata leaf-use forest is managed in intensive cultivation.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, relate to a kind of propagation method of Pasania cuspidata, the propagation method of especially a kind of Pasania cuspidata young anvil grafting container seedling.
Background technology
Pasania cuspidata (
lithocarpus polystachyus), have another name called many fringes C.-glauca, sweet tea, aiphyllium or dungarunga, high 7 ~ 15m, be the torrid zone, tropical trees, be distributed in each province on the south the Changjiang river, India, Thailand also have.Pasania cuspidata is valuable ingredients of traditional Chinese medicine seeds, the effective component mainly flavonoids of Pasania cuspidata, and its content is greater than 30 %, is wherein the emphasis that people pay close attention to the phloridzin of dihydrochalcone glycoside, trifloroside.Pasania cuspidata flavonoids has very strong anti-oxidant and plurality of health care functions, Recent study finds, Pasania cuspidata and extract thereof have the effect of significantly hypoglycemic and reducing blood lipid, the sugar refined with tender leaf can supply diabetes patients, has important facilitation to the control of the diabetes of common concern in the world and treatment.
Can pigment be extracted from the tender leaf of Pasania cuspidata, Pasania cuspidata pigment productive rate can reach cured leaf heavy about 10%, be brown granular shape highly finished product, nonhygroscopic rotten, can long term storage, free from extraneous odour, good water solubility, strong coloring force, to light, hot color stability, this pigment is tested through uremic toxins, acute toxicity experience, the nontoxic level in true border, edible safety is high, and the sugar-coat that can be used for candy, cola drink, ice cream, assembled alcoholic drinks and medicine is painted.Pasania cuspidata tender leaf and bud are used for being made into Pasania cuspidata " sweet tea " and drink, and color and luster, mouthfeel are good, existing history of more than one thousand years.The exploitation of Pasania cuspidata leaf have become the focus of current exploratory development.
At present, Pasania cuspidata is main mainly with seminal propagation, but seminal propagation needs the storing and germination accelerating process of experience more than 4 ~ 5 months, and seed sprouting is irregular, large with the descendants specialize of seminal propagation, is difficult to keep maternal merit.And Pasania cuspidata adopts normal cutting propagation propagation method to cultivate, breeding cycle is long, and rooting rate is unstable, and survival rate is not high, is unfavorable for the large-scale production that Pasania cuspidata is bred and Application and Development.Current Pasania cuspidata " sweet tea " raw material is mostly from the Pasania cuspidata plant of spontaneous growth, but due to resource dispersion, tree-like tall and big, picking blade is time-consuming takes a lot of work, and seriously constrains the large-scale development of the sweet tea of Pasania cuspidata.The breeding of Pasania cuspidata young anvil grafting container seedling has easy to operation, that reproduction speed is fast, survival rate is high feature; also maintain the excellent economic characters of Pasania cuspidata female parent simultaneously; tree-like dwarfing, is conducive to gathering of intensive cultivation and blade, scale can be applied to actual production.
Therefore, Pasania cuspidata extremely social concerns under the new situation, innovate a kind of vegetative method of Pasania cuspidata, promote Pasania cuspidata high-quality, efficient, scale is fast-developing significant.
Summary of the invention
The object of the present invention is to provide the excellent economic characters that a kind of cultivation period is short, reproduction speed fast, maintenance is maternal, tree-like dwarfing, the easy and simple to handle Pasania cuspidata young anvil grafting that utilizes breeds the method for nursery stock.
The present invention is achieved through the following technical solutions above-mentioned purpose:
A propagation method for Pasania cuspidata young anvil grafting container seedling, is characterized in that: comprise young anvil and cultivate and propagation by grafiting step, and obtain high-quality Pasania cuspidata nursery stock, its operating procedure is as follows:
1. young anvil is cultivated
(1) seedbed and container prepare: select leeward on the sunny side, place that drainage is good builds seedbed, container for plant growth is filled Nutrition Soil, neatly be placed on seedbed, the formula of Nutrition Soil is yellow mud by volume feed: coconut palm chaff=4 ~ 5: 5 ~ 6, mix thoroughly formulated, container for plant growth adopts the degradable nonwoven fabrics Seedling bag of 10 cm × 12 cm, sows first 1 day and carries out disinfection to Nutrition Soil with the potassium permanganate that weight concentration is 0.1 % routinely.
(2) seed treatment: select common Pasania cuspidata seed with Seed soaking 1 day before sowing, then is the chlorophos solution seed dressing of 0.2% by weight concentration, and covers plastic film and carry out desinsection, and then with wet husky stored seeds, volume ratio that is husky and seed is 1:1.
(3) sow: seed germinates to February next year to March after storing and germination accelerating after a while, is taken out by the seed of germination to sow from wet sand and cultivate stock in the container for plant growth filling Nutrition Soil, each container bag sows 1, and cm is thick for blinding 1.
(4) young anvil management: after planting regularly trickle moisturizing, nursery stock is unearthed and starts fertilising after 20 days, based on the aqueous solution of commercially available composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for 1st time is 0. 5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90 % and sprays pest control, carry out grafting when young anvil leading thread reaches 0.3 more than cm.
2. propagation by grafiting
(1) fringe bar prepares: on excellent Pasania cuspidata elite stand, gather semi-lignified branch as fringe bar, the blade of often opening on fringe bar cuts off half, and every 50 a bundles wrapping, carries out moisturizing with wet towel etc.
(2) grafting: reach the Pasania cuspidata seedling of 0.3 more than cm for stock with leading thread, adopt the grafting of cut-grafting method, first stock is being cut off from stock base portion 10 ~ 12 cm place with secateurs, vertically cut with the cross section of grafting knife at stock, the degree of depth reaches 1.5 cm, then fringe bar is cut into the long scion remaining with 1 ~ 2 bud of 3 ~ 4 cm, angle at 45 ° is all cut at scion two ends, angle at 45 ° is only cut in lower end by the top tip, the wound face that a 1.5cm is long is cut in the long face of scion lower end, cut the degree of depth and reach xylem, in the otch of rapid insertion stock, scion is alignd mutually with the formation layer of stock, bind with plastic film tape.
(3) managing after grafting: take shed after grafting and cover transparent plastic film moisturizing, and cover sunshade net to nursery stock shading, keep canopy humidity 85 ~ 90 %; Plastic film is opened, the rudiment in time removing stock grown after young sprout is extracted in scion out; After young sprout is aging, opens sunshade net gradually, under full light, cultivates grafting; Strengthen water and fertilizer management and the extermination of disease and insect pest seedling stage, method manages with stock, but stops fertilising after entering the winter, makes the abundant lignification of grafting, can go out garden afforestation to spring next year grafting.
the beneficial effect that the present invention gives prominence to is:
The present invention is the once breakthrough of Pasania cuspidata vegetative propagation technique, Pasania cuspidata children anvil is utilized to carry out propagation by grafiting, a large amount of Fast-propagation maintains the Pasania cuspidata nursery stock of maternal excellent economic characters, seedling root is flourishing, survival rate is high, and be applicable to downgrading intensive cultivation, promote Pasania cuspidata high-quality, efficient, fast-developing.
Embodiment
Below by way of concrete case study on implementation, technical scheme of the present invention is described further.
embodiment 1:
1. young anvil is cultivated
(1) seedbed and container prepare: select leeward on the sunny side, place that drainage is good builds seedbed, container for plant growth is filled Nutrition Soil to be neatly placed on seedbed, the formula of Nutrition Soil is that 4 parts of yellow muds and 6 parts of coconut palm chaffs are mixed thoroughly formulated by volume feed, container for plant growth adopts the degradable nonwoven fabrics Seedling bag of 10 cm × 12 cm, and sowing first 1 day is that the potassium permanganate of 0.1 % carries out sterilizing to Nutrition Soil by concentration.
(2) seed treatment: select the common Pasania cuspidata seed of gathering then in October, 2012, Seed soaking 1 day is used before sowing, dress seed with the chlorophos solution that concentration is 0.2% and cover plastic film again and carry out desinsection, then with wet husky stored seeds, husky is 1: 1 with the volume ratio of seed.
(3) sow: seed starts to germinate to February next year after storing and germination accelerating after a while, was taken out by the seed of germination February 15 to sow from wet sand and cultivate stock in the container for plant growth filling Nutrition Soil, each container bag sows 1, and cm is thick for blinding 1.
(4) young anvil management: after planting regularly trickle moisturizing, nursery stock is unearthed and starts fertilising after 20 days, based on composite fertilizer, weeding in every 15 days and fertilising 1 time, frequent trickle keeps Nutrition Soil moistening, monthly spray pest control with chlorophos 800 times of liquid of 90%, carry out grafting when young anvil leading thread reaches more than 0.3cm.
2. propagation by grafiting
(1) fringe bar gathers: on September 10th, 2013 gathers semi-lignified branch as fringe bar on excellent Pasania cuspidata elite stand, and the blade of often opening on fringe bar cuts off half, reduces water consumption, and every 50 a bundles wrapping, carries out moisturizing with wet towel etc.
(2) grafting: on September 10th, 2013 reaches the Pasania cuspidata seedling of 0.3 more than cm for stock with leading thread, adopt the grafting of cut-grafting method, first stock is being cut off from stock base portion 10 ~ 12 cm place with secateurs, vertically cut with the cross section of grafting knife at stock, the degree of depth reaches 1.5cm, then fringe bar is repaiied and be cut into the long scion remaining with 1 ~ 2 bud of 3 ~ 4 cm, angle at 45 ° is all cut at scion two ends, angle at 45 ° is only cut in lower end by the top tip, the wound face that a 1.5cm is long is repaiied in the long face of scion lower end, the degree of depth reaches xylem, in the otch of rapid insertion stock, scion is alignd mutually with the phloem of stock, pack tightly from bottom to top with plastic film tape.
(3) managing after grafting: take shed after grafting and cover transparent plastic film moisturizing, and cover sunshade net and shelter from heat or light to nursery stock, keep canopy humidity 85 ~ 90%; After young sprout is extracted in scion out, open plastic film, the rudiment in time removing stock grown, makes nutrition centralizedly supply grow to scion; First time young sprout aging after open sunshade net gradually, under full light, cultivate grafting; In November, 2013 checks that graft survival rate reaches 92.6%; Strengthen water and fertilizer management and the extermination of disease and insect pest seedling stage, method manages with stock, but stops fertilising after entering the winter, makes the abundant lignification of nursery stock, can go out garden afforestation to spring next year grafting.
embodiment 2:
In mid-March, 2014 gathers semi-lignified branch as fringe bar on excellent Pasania cuspidata elite stand, and the blade on fringe bar is cut off half, and every 50 a bundles wrapping, carries out moisturizing with wet towel etc.
On March 11st ~ 13,2014 reaches the Pasania cuspidata seedling of 0.3 more than cm for stock with leading thread, adopt the grafting of cut-grafting method, first stock is being cut off from stock base portion 10 ~ 12 cm place with secateurs, vertically cut with the cross section of grafting knife at stock, the degree of depth reaches 1.5cm, then fringe bar is repaiied and be cut into the long scion remaining with 1 ~ 2 bud of 3 ~ 4 cm, angle at 45 ° is all cut at scion two ends, angle at 45 ° is only cut in lower end by the top tip, the wound face that a 1.5cm is long is repaiied in the long face of scion lower end, the degree of depth reaches xylem, in the otch of rapid insertion stock, scion is alignd mutually with the phloem of stock, pack tightly from bottom to top with plastic film tape.
Take shed after grafting cover transparent plastic film and sunshade net moisturizing and shelter from heat or light, keep canopy humidity 85 ~ 90%; After young sprout is extracted in scion out, open plastic film, the rudiment in time removing stock grown, makes nutrition centralizedly supply grow to scion; First time young sprout aging after open sunshade net gradually, under full light, cultivate grafting; Within 2014, daily inspection in April 29 graft survival rate reaches 93.7%.
embodiment 3:
In December, 2012 selects common Pasania cuspidata seed, with Seed soaking 1 day before sowing, then is the chlorophos solution seed dressing of 0.2% by weight concentration, and covers plastic film and carry out desinsection, and then with wet husky stored seeds, volume ratio that is husky and seed is 1:1.Seed germinates in March, 2013 after storing and germination accelerating after a while, and is taken out by the seed of germination to sow from wet sand and cultivate stock in the container for plant growth filling Nutrition Soil, each container bag sows 1, and cm is thick for blinding 1.Seedbed is built in the place that before sowing, selection is leeward on the sunny side, drainage is good, container for plant growth is filled Nutrition Soil, neatly be placed on seedbed, the formula of Nutrition Soil is that 4 parts of yellow muds and 5 parts of coconut palm chaffs are mixed thoroughly formulated by volume feed, container for plant growth adopts the degradable nonwoven fabrics Seedling bag of 10 cm × 12 cm, sows first 1 day and carries out disinfection to Nutrition Soil with the potassium permanganate that weight concentration is 0.1 % routinely.
After planting regularly trickle moisturizing, nursery stock is unearthed and starts fertilising after 20 days, and with the aqueous solution of commercially available nursery stock with composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for the 1st time is 0.5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90 % and sprays pest control, carry out grafting when young anvil leading thread reaches 0.3 more than cm.
Gather on excellent Pasania cuspidata elite stand and gather semi-lignified branch as fringe bar, the blade of often opening on fringe bar cuts off half, and every 50 a bundles wrapping, carries out moisturizing with wet towel etc.Pasania cuspidata seedling leading thread being reached 0.3 more than cm is stock, adopt the grafting of cut-grafting method, first stock is being cut off from stock base portion 10 ~ 11 cm place with secateurs, vertically cut with the cross section of grafting knife at stock, the degree of depth reaches 1.5 cm, then fringe bar is cut into the long scion remaining with 1 ~ 2 bud of 3 ~ 4 cm, angle at 45 ° is all cut at scion two ends, angle at 45 ° is only cut in lower end by the top tip, the wound face that a 1.5cm is long is cut in the long face of scion lower end, cut the degree of depth and reach xylem, in the otch of rapid insertion stock, scion is alignd mutually with the formation layer of stock, bind with plastic film tape.Take shed after grafting and cover transparent plastic film moisturizing, and cover sunshade net to nursery stock shading, keep canopy humidity 85 ~ 90 %; Plastic film is opened, the rudiment in time removing stock grown after young sprout is extracted in scion out; After young sprout is aging, opens sunshade net gradually, under full light, cultivates grafting; Strengthen water and fertilizer management and the extermination of disease and insect pest seedling stage, method manages with stock, but stops fertilising after entering the winter, and make the abundant lignification of grafting, within 2014, daily inspection in April 26 graft survival rate reaches 94.48 %, and grafting goes out garden afforestation.
Claims (4)
1. a propagation method for Pasania cuspidata young anvil grafting container seedling, is characterized in that: comprise young anvil and cultivate and propagation by grafiting step, and obtain high-quality Pasania cuspidata nursery stock, its operating procedure is as follows:
(1) young anvil is cultivated
1. seedbed and container prepare: select leeward on the sunny side, the place of good water permeability builds seedbed, container for plant growth is filled Nutrition Soil, is neatly placed on seedbed, sow first 1 day and with the potassium permanganate that weight concentration is 0.1%, Nutrition Soil is carried out disinfection routinely;
2. seed treatment: select common Pasania cuspidata seed with Seed soaking 1 day before sowing, then is the chlorophos solution seed dressing of 0.2% by weight concentration, and covers plastic film and carry out desinsection, and then with wet husky stored seeds, volume ratio that is husky and seed is 1: 1;
3. sow: germinate February next year to March after seed storage vernalization, the seed of germination is taken out from wet sand sowing and cultivate stock in the container for plant growth filling Nutrition Soil, each container bag sows 1, and blinding 1cm is thick;
4. young anvil management: after planting regularly trickle moisturizing, nursery stock strengthens water and fertilizer management and the extermination of disease and insect pest after being unearthed, and seedling leading thread reaches more than 0.3cm for grafting;
(2) propagation by grafiting
1. fringe bar prepares: on excellent Pasania cuspidata elite stand, gather the fringe bar of semi-lignified branch as grafting, the blade of often opening on fringe bar cuts off half, and every 50 a bundles wrapping, carries out moisturizing with wet towel;
2. grafting: reach the Pasania cuspidata seedling of more than 0.3cm for stock with leading thread, adopts the grafting of cut-grafting method;
3. managing after grafting: take shed after grafting and cover overlay moisturizing, and cover sunshade net to nursery stock shading, keep canopy humidity 85 ~ 90%, after young sprout is extracted in scion out, open plastic film, the rudiment in time removing stock grown; After young sprout is aging, opens sunshade net gradually, under full light, cultivates grafting; Strengthen water and fertilizer management and the extermination of disease and insect pest seedling stage, method manages with stock, but stops fertilising after entering the winter, makes the abundant lignification of grafting, can go out garden afforestation to spring next year grafting.
2. the propagation method of a kind of Pasania cuspidata young anvil grafting container seedling according to claim 1, is characterized in that: the formula of described Nutrition Soil by volume feed is: yellow mud: coconut palm chaff=4 ~ 5: 5 ~ 6; Described container for plant growth adopts the degradable nonwoven fabrics Seedling bag of 10cm × 12cm.
3. the propagation method of a kind of Pasania cuspidata young anvil grafting container seedling according to claim 1, it is characterized in that: described water and fertilizer management and the extermination of disease and insect pest, fertilising is started after nursery stock is unearthed 20 days, based on the aqueous solution of commercially available composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for 1st time is 0.5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90% and sprays pest control.
4. the propagation method of a kind of Pasania cuspidata young anvil grafting container seedling according to claim 1, it is characterized in that: first described cut-grafting method grafting is cutting off stock from stock base portion 10 ~ 12cm place with secateurs, vertically cut with the cross section of grafting knife at stock, the degree of depth reaches 1.5cm, then fringe bar is cut into the long scion remaining with 1 ~ 2 bud of 3 ~ 4cm, angle at 45 ° is all cut at scion two ends, angle at 45 ° is only cut in lower end by the top tip, the wound face that a 1.5cm is long is cut in the long face of scion lower end, cut the degree of depth and reach xylem, in the otch of rapid insertion stock, scion is alignd mutually with the formation layer of stock, bind with plastic film tape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410207666.4A CN103975739B (en) | 2014-05-16 | 2014-05-16 | The propagation method of a kind of Pasania cuspidata young anvil grafting container seedling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410207666.4A CN103975739B (en) | 2014-05-16 | 2014-05-16 | The propagation method of a kind of Pasania cuspidata young anvil grafting container seedling |
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Application publication date: 20140813 Assignee: Guangxi Shengjin Agricultural Technology Co.,Ltd. Assignor: GUANGXI ZHUANG AUTONOMOUS REGION FORESTRY Research Institute Contract record no.: X2023980045763 Denomination of invention: A Method for Propagation of Container Seedlings Grafted with Young Rootstocks of Multi panicle Cocoa Granted publication date: 20150923 License type: Common License Record date: 20231106 |
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